BBa_K1620004
1
Zur
Zinc uptake regulation protein - Zur
2015-09-02T11:00:00Z
2015-09-13T03:57:27Z
Designed from gene "zur" from E. coli K12 MG-1655 genome. Synthesized as G-block by IDT. PCR amplified using following primers:
Fwd:
5' - GAA TTC GCG GCC GCT TCT AGA GAT GGA AAA GAC CAC AAC GCA - 3'
Rev:
5' - TAC TAG TAG CGG CCG CTG CAG TTA ACG CGG TTT CTT TTT CA - 3'
The assembly was made through 3A assembly method.
Acts like a negative controlling element of promoter Zasp (BBa_K1620001) by use of Zn(II) as a cofactor to bind the operator of the repressed genes (znuACB). In our project it was used to regulate a kill switch based on zinc concentration to promote cellular death.
false
false
_2037_
23597
23597
9
false
The designed gene was as provided by E. coli genome, with the same codon frequencies.
false
Celio Dias Santos Jr
annotation2443233
1
Rev
range2443233
1
499
519
annotation2443232
1
Fwd
range2443232
1
1
20
annotation2443231
1
Zur
range2443231
1
1
519
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_S05282
1
BBa_S05282
B0030:K1620004
2015-09-02T11:00:00Z
2015-09-03T03:01:35Z
false
false
_9_
23597
23597
9
false
false
Celio Dias Santos Jr
component2443270
1
BBa_B0030
component2443275
1
BBa_K1620004
annotation2443275
1
BBa_K1620004
range2443275
1
22
540
annotation2443270
1
BBa_B0030
range2443270
1
1
15
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1620004_sequence
1
atggaaaagaccacaacgcaggagttattagcgcaggctgaaaaaatctgcgcgcagcgtaatgtgcgcctgaccccacagcgcctggaagtgttgcgcctgatgagtctgcaagatggcgctatcagcgcttatgatctgcttgatttactgcgcgaagctgaaccgcaagccaagccgccaacggtttatcgcgcgctggattttctgcttgagcaaggttttgtgcataaggtggaatccaccaacagttatgtgctctgtcatctgttcgatcagcccacccatacgtcagccatgtttatttgcgatcgctgcggcgcagtgaaagaagagtgtgcagaaggcgtggaagacattatgcatacgctggcggcaaaaatggggtttgccctgcggcataatgtgattgaagcacatgggctctgtgcggcatgtgtagaagtggaagcgtgtcgtcatcctgaacagtgccagcatgatcactctgtgcaggtgaaaaagaaaccgcgttaataa
BBa_S05282_sequence
1
attaaagaggagaaatactagatggaaaagaccacaacgcaggagttattagcgcaggctgaaaaaatctgcgcgcagcgtaatgtgcgcctgaccccacagcgcctggaagtgttgcgcctgatgagtctgcaagatggcgctatcagcgcttatgatctgcttgatttactgcgcgaagctgaaccgcaagccaagccgccaacggtttatcgcgcgctggattttctgcttgagcaaggttttgtgcataaggtggaatccaccaacagttatgtgctctgtcatctgttcgatcagcccacccatacgtcagccatgtttatttgcgatcgctgcggcgcagtgaaagaagagtgtgcagaaggcgtggaagacattatgcatacgctggcggcaaaaatggggtttgccctgcggcataatgtgattgaagcacatgggctctgtgcggcatgtgtagaagtggaagcgtgtcgtcatcctgaacagtgccagcatgatcactctgtgcaggtgaaaaagaaaccgcgttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z