BBa_S05300
type: ComponentDefinition
persistent identity: BBa S05300
display id: BBa_S05300
version: 1
was generated by: iGEM to SBOL conversion
description: I742111:E0240
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T02:50:32Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 16902
owner id: 16902
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Şeniz Y??ksel
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0240
component: BBa_I742111
sequence annotation: BBa_I742111
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_S05300_sequence/1

BBa_E0240
type: Component
persistent identity: component2457910
display id: component2457910
version: 1
top level: BBa_S05300
definition: GFP report
access: public

BBa_I742111
type: Component
persistent identity: component2457899
display id: component2457899
version: 1
top level: BBa_S05300
definition: rbs+LIMS1
access: public

BBa_I742111
type: SequenceAnnotation
persistent identity: annotation2457899
display id: annotation2457899
version: 1
top level: BBa_S05300
location: https://synbiohub.org/public/igem/BBa_S05300/annotation2457899/range2457899/1
component: BBa_I742111

https://synbiohub.org/public/igem/BBa_S05300/annotation2457899/range2457899/1
type: Range
persistent identity: range2457899
display id: range2457899
version: 1
top level: BBa_S05300
direction: direction/reverse
start: 1
end: 1687
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2457910
display id: annotation2457910
version: 1
top level: BBa_S05300
location: https://synbiohub.org/public/igem/BBa_S05300/annotation2457910/range2457910/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_S05300/annotation2457910/range2457910/1
type: Range
persistent identity: range2457910
display id: range2457910
version: 1
top level: BBa_S05300
direction: direction/reverse
start: 1696
end: 2571
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

rbs+LIMS1
type: ComponentDefinition
persistent identity: BBa I742111
display id: BBa_I742111
version: 1
was generated by: iGEM to SBOL conversion
description: Limonene synthase gene with ribosome binding site
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Ribosome binding site attached to Limonene synthase gene. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway). (+) Limonene is a component of lemon scent.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG. LIMS cDNA was used as genomic DNA contains introns.
star: true
creator: sarah hollingshead
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742110
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_I742110
sequence: https://synbiohub.org/public/igem/BBa_I742111_sequence/1

BBa_I742110
type: Component
persistent identity: component1955374
display id: component1955374
version: 1
top level: rbs+LIMS1
definition: LIMS1
access: public

BBa_J15001
type: Component
persistent identity: component1955370
display id: component1955370
version: 1
top level: rbs+LIMS1
definition: BBa_J15001
access: public

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1955370
display id: annotation1955370
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
type: Range
persistent identity: range1955370
display id: range1955370
version: 1
top level: rbs+LIMS1
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1955374
display id: annotation1955374
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
type: Range
persistent identity: range1955374
display id: range1955374
version: 1
top level: rbs+LIMS1
direction: direction/reverse
start: 17
end: 1687
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

LIMS1
type: ComponentDefinition
persistent identity: BBa I742110
display id: BBa_I742110
version: 1
was generated by: iGEM to SBOL conversion
description: LIMS1
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
mutable description: Limonene synthase 1 from Citrus limon (accession AF514287) Synthesised by Geneart. Limonene synthase converts farnesyl-diphosphate to + limonene, which is a component of lemon scent.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: (+) limonene synthase coding region
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I742110_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1949773
display id: annotation1949773
version: 1
top level: LIMS1
location: https://synbiohub.org/public/igem/BBa_I742110/annotation1949773/range1949773/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I742110/annotation1949773/range1949773/1
type: Range
persistent identity: range1949773
display id: range1949773
version: 1
top level: LIMS1
direction: direction/forward
start: 1
end: 3
orientation: inline

(+) limonene synthase coding region
type: SequenceAnnotation
persistent identity: annotation1949772
display id: annotation1949772
version: 1
top level: LIMS1
location: https://synbiohub.org/public/igem/BBa_I742110/annotation1949772/range1949772/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742110/annotation1949772/range1949772/1
type: Range
persistent identity: range1949772
display id: range1949772
version: 1
top level: LIMS1
direction: direction/forward
start: 1
end: 1671
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1949774
display id: annotation1949774
version: 1
top level: LIMS1
location: https://synbiohub.org/public/igem/BBa_I742110/annotation1949774/range1949774/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I742110/annotation1949774/range1949774/1
type: Range
persistent identity: range1949774
display id: range1949774
version: 1
top level: LIMS1
direction: direction/forward
start: 1666
end: 1671
orientation: inline

https://synbiohub.org/public/igem/BBa_S05300_sequence/1
type: Sequence
persistent identity: BBa S05300 sequence
display id: BBa_S05300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggaggtactagatgcgtcgttcagcaaactaccaaccttcaatttgggaccacgattttttgcagtcattgaatagcaactatacggatgaagcatacaaaagacgagcagaagagctgaggggaaaagtgaagatagcgattaaggatgtaatcgagcctctggatcagttggagctgattgataacttgcaaagacttggattggctcatcgttttgagactgagattaggaacatattgaataatatctacaacaataataaagattataattggagaaaagaaaatctgtatgcaacctcccttgagtttagactacttagacaacatggctatcctgtttctcaagaggttttcaatggttttaaagacgaccagggaggcttcatttgtgatgatttcaagggaatactgagcttgcatgaagcttcgtattacagcttagaaggagaaagcatcatggaggaggcctggcaatttaccagcaaacatcttaaagaagtgatgatcagcaagaacatggaagaggatgtatttgtagcagaacaagcgaagcgtgcactggaactgcctctgcattggaaagtgccaatgttagaggcaaggtggttcatacacatttatgagagaagagaggacaagaaccaccttttacttgaactggctaagatggagtttaacactttgcaggcaatttaccaggaagaactaaaagaaatttcagggtggtggaaggatacaggtcttggagagaaattgagctttgcgaggaacaggttggtagcgtccttcttatggagcatggggatcgcgtttgagcctcaattcgcctattgtcggagagtgctcacaatctcgatagccctaattacagtgattgatgacatttatgatgtctatggaacattggatgaacttgagatattcactgatgctgttgagaggtgggacatcaattatgctttgaagcaccttccgggctatatgaaaatgtgttttcttgcgctttacaactttgttaatgaatttgcttattacgttctcaaacaacaggattttgatttgcttctgagcataaaaaatgcatggcttggcttaatacaagcctacttggtggaggcgaaatggtaccatagcaagtacacaccgaaactggaagaatacttggaaaatggattggtatcaataacgggccctttaattataacgatttcatatctttctggtacaaatccaatcattaagaaggaactggaatttctggaaagtaatccagatatagttcactggtcatccaagattttccgtctgcaagatgatttgggaacttcatcggacgagatacagagaggggatgttccgaaatcaatccagtgttacatgcatgaaactggtgcctcagaggaagttgctcgtcaacacatcaaggatatgatgagacagatgtggaagaaggtgaatgcatacacagccgataaagactctcccttgactggaacaactactgagttcctcttgaatcttgtgagaatgtcccattttatgtatctacatggagatgggcatggtgttcaaaaccaagagactatcgatgtcggttttacattgctttttcagcccattcccttggaggacaaacacatggctttcacagcatctcctggcaccaaaggctaataatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742111_sequence/1
type: Sequence
persistent identity: BBa I742111 sequence
display id: BBa_I742111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggaggtactagatgcgtcgttcagcaaactaccaaccttcaatttgggaccacgattttttgcagtcattgaatagcaactatacggatgaagcatacaaaagacgagcagaagagctgaggggaaaagtgaagatagcgattaaggatgtaatcgagcctctggatcagttggagctgattgataacttgcaaagacttggattggctcatcgttttgagactgagattaggaacatattgaataatatctacaacaataataaagattataattggagaaaagaaaatctgtatgcaacctcccttgagtttagactacttagacaacatggctatcctgtttctcaagaggttttcaatggttttaaagacgaccagggaggcttcatttgtgatgatttcaagggaatactgagcttgcatgaagcttcgtattacagcttagaaggagaaagcatcatggaggaggcctggcaatttaccagcaaacatcttaaagaagtgatgatcagcaagaacatggaagaggatgtatttgtagcagaacaagcgaagcgtgcactggaactgcctctgcattggaaagtgccaatgttagaggcaaggtggttcatacacatttatgagagaagagaggacaagaaccaccttttacttgaactggctaagatggagtttaacactttgcaggcaatttaccaggaagaactaaaagaaatttcagggtggtggaaggatacaggtcttggagagaaattgagctttgcgaggaacaggttggtagcgtccttcttatggagcatggggatcgcgtttgagcctcaattcgcctattgtcggagagtgctcacaatctcgatagccctaattacagtgattgatgacatttatgatgtctatggaacattggatgaacttgagatattcactgatgctgttgagaggtgggacatcaattatgctttgaagcaccttccgggctatatgaaaatgtgttttcttgcgctttacaactttgttaatgaatttgcttattacgttctcaaacaacaggattttgatttgcttctgagcataaaaaatgcatggcttggcttaatacaagcctacttggtggaggcgaaatggtaccatagcaagtacacaccgaaactggaagaatacttggaaaatggattggtatcaataacgggccctttaattataacgatttcatatctttctggtacaaatccaatcattaagaaggaactggaatttctggaaagtaatccagatatagttcactggtcatccaagattttccgtctgcaagatgatttgggaacttcatcggacgagatacagagaggggatgttccgaaatcaatccagtgttacatgcatgaaactggtgcctcagaggaagttgctcgtcaacacatcaaggatatgatgagacagatgtggaagaaggtgaatgcatacacagccgataaagactctcccttgactggaacaactactgagttcctcttgaatcttgtgagaatgtcccattttatgtatctacatggagatgggcatggtgttcaaaaccaagagactatcgatgtcggttttacattgctttttcagcccattcccttggaggacaaacacatggctttcacagcatctcctggcaccaaaggctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742110_sequence/1
type: Sequence
persistent identity: BBa I742110 sequence
display id: BBa_I742110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtcgttcagcaaactaccaaccttcaatttgggaccacgattttttgcagtcattgaatagcaactatacggatgaagcatacaaaagacgagcagaagagctgaggggaaaagtgaagatagcgattaaggatgtaatcgagcctctggatcagttggagctgattgataacttgcaaagacttggattggctcatcgttttgagactgagattaggaacatattgaataatatctacaacaataataaagattataattggagaaaagaaaatctgtatgcaacctcccttgagtttagactacttagacaacatggctatcctgtttctcaagaggttttcaatggttttaaagacgaccagggaggcttcatttgtgatgatttcaagggaatactgagcttgcatgaagcttcgtattacagcttagaaggagaaagcatcatggaggaggcctggcaatttaccagcaaacatcttaaagaagtgatgatcagcaagaacatggaagaggatgtatttgtagcagaacaagcgaagcgtgcactggaactgcctctgcattggaaagtgccaatgttagaggcaaggtggttcatacacatttatgagagaagagaggacaagaaccaccttttacttgaactggctaagatggagtttaacactttgcaggcaatttaccaggaagaactaaaagaaatttcagggtggtggaaggatacaggtcttggagagaaattgagctttgcgaggaacaggttggtagcgtccttcttatggagcatggggatcgcgtttgagcctcaattcgcctattgtcggagagtgctcacaatctcgatagccctaattacagtgattgatgacatttatgatgtctatggaacattggatgaacttgagatattcactgatgctgttgagaggtgggacatcaattatgctttgaagcaccttccgggctatatgaaaatgtgttttcttgcgctttacaactttgttaatgaatttgcttattacgttctcaaacaacaggattttgatttgcttctgagcataaaaaatgcatggcttggcttaatacaagcctacttggtggaggcgaaatggtaccatagcaagtacacaccgaaactggaagaatacttggaaaatggattggtatcaataacgggccctttaattataacgatttcatatctttctggtacaaatccaatcattaagaaggaactggaatttctggaaagtaatccagatatagttcactggtcatccaagattttccgtctgcaagatgatttgggaacttcatcggacgagatacagagaggggatgttccgaaatcaatccagtgttacatgcatgaaactggtgcctcagaggaagttgctcgtcaacacatcaaggatatgatgagacagatgtggaagaaggtgaatgcatacacagccgataaagactctcccttgactggaacaactactgagttcctcttgaatcttgtgagaatgtcccattttatgtatctacatggagatgggcatggtgttcaaaaccaagagactatcgatgtcggttttacattgctttttcagcccattcccttggaggacaaacacatggctttcacagcatctcctggcaccaaaggctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z