BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K1685005
1
BBa_K1685005
aeBlue
2015-09-15T11:00:00Z
2016-01-25T01:09:54Z
The aeBlue sequence from Uppsala team 2012 (BBa_K864401) was chemically synthesised.
This part encodes a blue chromoprotein named aeBlue. This part is useful for reporter.
false
false
_2103_
4206
25100
9
false
The part has been codon-optimised for expression in E. coli using http://sg.idtdna.com/CodonOpt
false
ITB_Indonesia 2015
BBa_S05301
1
BBa_S05301
K1685005:B0015
2015-09-15T11:00:00Z
2015-09-16T04:37:11Z
false
false
_9_
25100
25100
9
false
false
Rahmat Azhari Kemal
component2459140
1
BBa_K1685005
component2459147
1
BBa_B0015
annotation2459147
1
BBa_B0015
range2459147
1
708
836
annotation2459140
1
BBa_K1685005
range2459140
1
1
699
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1685005_sequence
1
atggcctccctggtgaagaaagatatgtgcattaagatgaccatggaagggacggtcaacggccatcactttaagtgcgtgggagaaggggaaggtaaaccgtttgaagggacccaagttgagaaaattcgtatcacggagggaggccctctgccatttgcctatgatatcctcgcaccatgctgtatgtatggtagcaagacgttcattaaacatgtgagcggtatcccggattatttcaaagagtctttcccagaaggctttacctgggaacgtacgcagatctttgaagacggggggtatttgactattcatcaggacaccagtttgcaagggaataacttcattttcaaggtaaacgtcatcggtgcgaactttccggccaatggaccggttatgcagaagaaaacagcgggttgggaaccgtgtgttgagatgctttatccgcgcgatggtgtgctgtgtgggcagtctctgatggcattgaagtgcacggatggcaatcatttaaccagccatttgcgtactacctaccgctcccgtaagccatcaaacgcagttaatatgccggaatttcattttggtgaccaccgtattgaaatcctgaaagcggaacagggaaaattttatgaacagtatgaatcggcggtagctcgttattgtgaagcagcaccgagtaaactgggtcatcactaataa
BBa_S05301_sequence
1
atggcctccctggtgaagaaagatatgtgcattaagatgaccatggaagggacggtcaacggccatcactttaagtgcgtgggagaaggggaaggtaaaccgtttgaagggacccaagttgagaaaattcgtatcacggagggaggccctctgccatttgcctatgatatcctcgcaccatgctgtatgtatggtagcaagacgttcattaaacatgtgagcggtatcccggattatttcaaagagtctttcccagaaggctttacctgggaacgtacgcagatctttgaagacggggggtatttgactattcatcaggacaccagtttgcaagggaataacttcattttcaaggtaaacgtcatcggtgcgaactttccggccaatggaccggttatgcagaagaaaacagcgggttgggaaccgtgtgttgagatgctttatccgcgcgatggtgtgctgtgtgggcagtctctgatggcattgaagtgcacggatggcaatcatttaaccagccatttgcgtactacctaccgctcccgtaagccatcaaacgcagttaatatgccggaatttcattttggtgaccaccgtattgaaatcctgaaagcggaacagggaaaattttatgaacagtatgaatcggcggtagctcgttattgtgaagcagcaccgagtaaactgggtcatcactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z