BBa_S05304 1 BBa_S05304 B0034:K1808000 2015-09-15T11:00:00Z 2015-09-16T11:43:55Z false false _9_ 26125 26125 9 false false D??ire Gannon component2461428 1 BBa_B0034 component2461430 1 BBa_K1808000 annotation2461430 1 BBa_K1808000 range2461430 1 19 1182 annotation2461428 1 BBa_B0034 range2461428 1 1 12 BBa_K1808000 1 BBa_K1808000 DBR2 gene from Artemisia annua 2015-09-15T11:00:00Z 2015-09-16T08:55:06Z Artemissia annua This is the coding region of the Dbr2 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells. false false _2233_ 26113 26113 9 false This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. false Arnas Petrauskas annotation2460888 1 DBR2 range2460888 1 1 1164 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S05304_sequence 1 aaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1808000_sequence 1 atgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z