BBa_S05303
1
BBa_S05303
B0032:K1808004
2015-09-15T11:00:00Z
2015-09-16T11:34:09Z
false
false
_9_
26125
26125
9
false
false
D??ire Gannon
component2461386
1
BBa_K1808004
component2461382
1
BBa_B0032
annotation2461382
1
BBa_B0032
range2461382
1
1
13
annotation2461386
1
BBa_K1808004
range2461386
1
20
1537
BBa_K1808004
1
BBa_K1808004
ALDH1 from Artemisia annua, 6 His tagged
2015-09-15T11:00:00Z
2015-09-18T06:12:06Z
Artemisia annua
This is the coding region of the ALDH1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NADP+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
false
false
_2233_
26113
26113
9
true
This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification.
false
Arnas Petrauskas
annotation2461246
1
6 His
range2461246
1
1497
1515
annotation2461243
1
ALDH1 6 His tagged
range2461243
1
1
1518
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_S05306
1
BBa_S05306
S05303:B0015
2015-09-15T11:00:00Z
2015-09-16T11:47:49Z
false
false
_9_
26113
26113
9
false
false
Arnas Petrauskas
component2461465
1
BBa_B0015
component2461458
1
BBa_S05303
annotation2461465
1
BBa_B0015
range2461465
1
1546
1674
annotation2461458
1
BBa_S05303
range2461458
1
1
1537
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0032_sequence
1
tcacacaggaaag
BBa_S05306_sequence
1
tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_S05303_sequence
1
tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1808004_sequence
1
atgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z