##gff-version 3
##sequence-region BBa_S05307 1 1568
BBa_S05307	.	engineered_region	729	1568	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_S05307	.	terminator	1440	1519	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_S05307	.	stem_loop	1451	1494	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05307	.	engineered_region	1440	1519	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05307	.	ribosome_entry_site	729	740	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_S05307	.	conserved_region	733	736	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05307	.	terminator	1528	1568	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_S05307	.	polyA_site	1555	1568	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05307	.	stem_loop	1535	1554	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05307	.	engineered_region	1528	1568	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05307	.	stop_codon	1561	1561	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05307	.	CDS	747	1406	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_S05307	.	CDS	750	1366	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S05307	.	engineered_tag	1407	1431	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S05307	.	tag	1367	1400	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S05307	.	CDS	1	720	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_S05307	.	CDS	1	720	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_S05307
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaa
cagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaact
acaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaaga
agatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaag
aatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaa
atactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcc
caacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatac
aaataataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattaga
gctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattg
tattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactttt
gccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcg
cgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttttta
tgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattgg
aagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagc
tatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaa
caacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata