BBa_S05313 1 BBa_S05313 K525998:K1601004 2015-09-17T11:00:00Z 2015-09-18T06:01:27Z false false _9_ 26607 26607 9 false false Marion Aruanno component2474545 1 BBa_K525998 component2474546 1 BBa_K1601004 annotation2474545 1 BBa_K525998 range2474545 1 1 32 annotation2474546 1 BBa_K1601004 range2474546 1 33 1419 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129433 1 RBS range2129433 1 24 27 annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2127788 1 B0034 range2127788 1 21 32 BBa_K1601004 1 BBa_K1601004 Laccase T.Thermophilus (Bba_K863011) without stop codon 2015-09-17T11:00:00Z 2015-09-18T05:37:05Z iGEM Laccase T.Thermophilus (Bba_K863011 from iGEM) without stop codon false false _2018_ 26607 26607 9 false We remove the stop codon using PCR false Marion Aruanno BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1601004_sequence 1 aatgctcgcacgccgttcctttctgcaagctgccgctggctcactggttttaggactggctcgggctcagggcccgtcatttccggagcccaaagttgtccgctcacagggtggcctgctgagcctgaagctgagtgccaccccgacaccgctggcgttggcaggtcagcgtgcgactctgttgacgtacggtggctcattccctggtccaaccctgcgtgtgcgtcctcgtgatacggtgcgcttaacactggaaaaccgtctgccagagcccacaaatctgcattggcacggtctgccgatttcgccgaaagtggatgatccgtttctggagatcccgccgggtgagtcctggacctacgaatttacggtgccgaaagaactggcgggaactttttggtaccacccgcacctgcacggtcgcgtagccccgcaactgtttgcgggtctgctgggcgccctcgttgtggagtcaagcctggatgctattccggagttacgcgaagccgaggagcacctgttggtgttaaaagatctggcgctgcaaggtggtcgtccggccccacacactccaatggactggatgaacggcaaagaaggcgacttagtcctggtcaatggcgcactgcgtccgacgctggtggcgcagaaggcgacgcttcgtctgcgtctgctgaacgcgagcaatgcgcggtattatcgcttggcactgcaagaccatccgctgtatctcatcgcggcggacggtggatttttagaggagcctctggaagtgtctgagctgctgctggccccgggtgaacgcgctgaggttttagtgcggcttcgtaaagagggccgctttttattacaggcgttaccatatgatcgcggggccatgggcatgatggatatgggtggcatggctcatgcaatgccgcaaggtccgagtcgcccggagacgctgctgtatctgatcgcgcccaaaaaccctaaaccactgccgcttccgaaagcgttgagcccatttcctactctcccggcgccggtcgtcacgcgtcgcctggtactgaccgaagacatgatggcggcgcgcttcttcattaacggtcaagtttttgaccatcgtcgtgtggatctgaaaggccaggcccagaccgtagaagtctgggaggttgaaaaccagggtgacatggaccaccctttccatctccatgtgcacccgtttcaagtgctgtccgtggggggccgtccatttccgtaccgcgcatggaaggacgtggttaacttgaaagccggcgaagttgcgcgcttgctggtccctttgcgcgagaaaggccgtaccgtttttcattgccatattgtggaacacgaggatcgtggcatgatgggtgttctggaagtgggc BBa_S05313_sequence 1 taatacgactcactatagggaaagaggagaaaaatgctcgcacgccgttcctttctgcaagctgccgctggctcactggttttaggactggctcgggctcagggcccgtcatttccggagcccaaagttgtccgctcacagggtggcctgctgagcctgaagctgagtgccaccccgacaccgctggcgttggcaggtcagcgtgcgactctgttgacgtacggtggctcattccctggtccaaccctgcgtgtgcgtcctcgtgatacggtgcgcttaacactggaaaaccgtctgccagagcccacaaatctgcattggcacggtctgccgatttcgccgaaagtggatgatccgtttctggagatcccgccgggtgagtcctggacctacgaatttacggtgccgaaagaactggcgggaactttttggtaccacccgcacctgcacggtcgcgtagccccgcaactgtttgcgggtctgctgggcgccctcgttgtggagtcaagcctggatgctattccggagttacgcgaagccgaggagcacctgttggtgttaaaagatctggcgctgcaaggtggtcgtccggccccacacactccaatggactggatgaacggcaaagaaggcgacttagtcctggtcaatggcgcactgcgtccgacgctggtggcgcagaaggcgacgcttcgtctgcgtctgctgaacgcgagcaatgcgcggtattatcgcttggcactgcaagaccatccgctgtatctcatcgcggcggacggtggatttttagaggagcctctggaagtgtctgagctgctgctggccccgggtgaacgcgctgaggttttagtgcggcttcgtaaagagggccgctttttattacaggcgttaccatatgatcgcggggccatgggcatgatggatatgggtggcatggctcatgcaatgccgcaaggtccgagtcgcccggagacgctgctgtatctgatcgcgcccaaaaaccctaaaccactgccgcttccgaaagcgttgagcccatttcctactctcccggcgccggtcgtcacgcgtcgcctggtactgaccgaagacatgatggcggcgcgcttcttcattaacggtcaagtttttgaccatcgtcgtgtggatctgaaaggccaggcccagaccgtagaagtctgggaggttgaaaaccagggtgacatggaccaccctttccatctccatgtgcacccgtttcaagtgctgtccgtggggggccgtccatttccgtaccgcgcatggaaggacgtggttaacttgaaagccggcgaagttgcgcgcttgctggtccctttgcgcgagaaaggccgtaccgtttttcattgccatattgtggaacacgaggatcgtggcatgatgggtgttctggaagtgggc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z