LOCUS BBa_S05321 3448 bp DNA linear 26-Dec-2024 DEFINITION R0062:K274100 ACCESSION BBa_S05321 VERSION BBa_S05321.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 RBS 64..67 /label=BBa_K118012 CDS 74..985 /label=BBa_I742151 misc_feature 994..996 /label=SacI RBS 997..1003 /label=rbs RBS 994..1003 /label=BBa_J15001 CDS 1010..1939 /label=crtB coding sequence CDS 1010..1942 /label=BBa_K118002 misc_feature 1951..1953 /label=SacI RBS 1954..1960 /label=rbs RBS 1951..1960 /label=BBa_J15001 misc_feature 2444..2449 /label=PstI site removed misc_feature 2855..2860 /label=PstI site removed CDS 1967..3445 /label=crtI coding sequence CDS 1967..3448 /label=BBa_K118003 misc_feature 64..3448 /label=BBa_K274100 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gaggaggtac tagatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc 121 ggagcagtta ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga 181 acgggatgtt gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg 241 ccccatgttg ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt 301 actggatttg gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat 361 gccctgcatg gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg 421 agagcatgtg gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc 481 cgatgcagat ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc 541 catcggcatg caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc 601 gcgcagcgct gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc 661 ctccatgcag atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca 721 tcgtttttca cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat 781 gaccgacacc ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt 841 aggcccgagg gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct 901 ctctgcggcc tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa 961 aaaactcgct gccgtcagtt aataatacta gagctcaagg aggtactaga tgaataatcc 1021 gtcgttactc aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc 1081 ctcaaagtta tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg 1141 ccattgtgac gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt 1201 acaaacgccc gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg 1261 atcgcagatg cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat 1321 cgccccggct tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca 1381 atacagccaa ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt 1441 gatgatggcg caaatcatgg gcgtgcggga taacgccacg ctggaccgcg cctgtgacct 1501 tgggctggca tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg 1561 ccgctgttat ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc 1621 ggcacctgaa aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga 1681 accttactat ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc 1741 aatcgctacg gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca 1801 gcaagcctgg gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc 1861 cgcctctggt caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct 1921 ctggcagcgc ccgctctaat aatactagag ctcaaggagg tactagatga aaccaactac 1981 ggtaattggt gcaggcttcg gtggcctggc actggcaatt cgtctacaag ctgcggggat 2041 ccccgtctta ctgcttgaac aacgtgataa acccggcggt cgggcttatg tctacgagga 2101 tcaggggttt acctttgatg caggcccgac ggttatcacc gatcccagtg ccattgaaga 2161 actgtttgca ctggcaggaa aacagttaaa agagtatgtc gaactgctgc cggttacgcc 2221 gttttaccgc ctgtgttggg agtcagggaa ggtctttaat tacgataacg atcaaacccg 2281 gctcgaagcg cagattcagc agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct 2341 ggactattca cgcgcggtgt ttaaagaagg ctatctaaag ctcggtactg tccctttttt 2401 atcgttcaga gacatgcttc gcgccgcacc tcaactggcg aaactgcaag catggagaag 2461 cgtttacagt aaggttgcca gttacatcga agatgaacat ctgcgccagg cgttttcttt 2521 ccactcgctg ttggtgggcg gcaatccctt cgccacctca tccatttata cgttgataca 2581 cgcgctggag cgtgagtggg gcgtctggtt tccgcgtggc ggcaccggcg cattagttca 2641 ggggatgata aagctgtttc aggatctggg tggcgaagtc gtgttaaacg ccagagtcag 2701 ccatatggaa acgacaggaa acaagattga agccgtgcat ttagaggacg gtcgcaggtt 2761 cctgacgcaa gccgtcgcgt caaatgcaga tgtggttcat acctatcgcg acctgttaag 2821 ccagcaccct gccgcggtta agcagtccaa caaactgcaa actaagcgca tgagtaactc 2881 tctgtttgtg ctctattttg gtttgaatca ccatcatgat cagctcgcgc atcacacggt 2941 ttgtttcggc ccgcgttacc gcgagctgat tgacgaaatt tttaatcatg atggcctcgc 3001 agaggacttc tcactttatc tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga 3061 aggttgcggc agttactatg tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga 3121 ctggacggtt gaggggccaa aactacgcga ccgtattttt gcgtaccttg agcagcatta 3181 catgcctggc ttacggagtc agctggtcac gcaccggatg tttacgccgt ttgattttcg 3241 cgaccagctt aatgcctatc atggctcagc cttttctgtg gagcccgttc ttacccagag 3301 cgcctggttt cggccgcata accgcgataa aaccattact aatctctacc tggtcggcgc 3361 aggcacgcat cccggcgcag gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg 3421 tttgatgctg gaggatctga tataataa //