LOCUS BBa_S05322 4614 bp DNA linear 29-Nov-2024 DEFINITION S05321:K546003 ACCESSION BBa_S05322 VERSION BBa_S05322.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 RBS 64..67 /label=BBa_K118012 CDS 74..985 /label=BBa_I742151 misc_feature 994..996 /label=SacI RBS 997..1003 /label=rbs RBS 994..1003 /label=BBa_J15001 CDS 1010..1939 /label=crtB coding sequence CDS 1010..1942 /label=BBa_K118002 misc_feature 1951..1953 /label=SacI RBS 1954..1960 /label=rbs RBS 1951..1960 /label=BBa_J15001 misc_feature 2444..2449 /label=PstI site removed misc_feature 2855..2860 /label=PstI site removed CDS 1967..3445 /label=crtI coding sequence CDS 1967..3448 /label=BBa_K118003 misc_feature 64..3448 /label=BBa_K274100 misc_feature 1..3448 /label=BBa_S05321 misc_feature 3457..3476 /label=LuxR/HSL promoter 3571..3578 /label=-10 promoter 3545..3550 /label=-35 misc_feature 3584..3584 /label=start misc_feature 3586..3605 /label=Putative LuxR/HSL misc_feature 3457..3607 /label=BBa_R0063 promoter 3457..3607 /label=BBa_R0063 misc_feature 3620..3623 /label=conserved RBS 3616..3627 /label=BBa_B0034 misc_feature 3634..3635 /label=prefix misc_feature 3807..3807 /label=T misc_feature 4125..4125 /label=A CDS 3634..4383 /label=luxR misc_feature 3634..4389 /label=BBa_C0062 misc_feature 4390..4414 /label=Help:Barcodes CDS 3634..4389 /label=BBa_C0062 stem_loop 4434..4477 /label=stem_loop misc_feature 4423..4502 /label=BBa_B0010 terminator 4423..4502 /label=BBa_B0010 stem_loop 4518..4537 /label=T7 TE misc_feature 4544..4544 /label=stop polyA_site 4538..4551 /label=polya misc_feature 4511..4551 /label=BBa_B0012 terminator 4511..4551 /label=BBa_B0012 terminator 4423..4551 /label=BBa_B0015 misc_feature 4560..4579 /label=LuxR/HSL promoter 4579..4584 /label=-35 promoter 4601..4606 /label=-10 misc_feature 4612..4612 /label=start misc_feature 4560..4614 /label=BBa_R0062 promoter 4560..4614 /label=BBa_R0062 misc_feature 3616..4614 /label=BBa_K317037 misc_feature 3457..4614 /label=BBa_K546003 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gaggaggtac tagatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc 121 ggagcagtta ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga 181 acgggatgtt gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg 241 ccccatgttg ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt 301 actggatttg gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat 361 gccctgcatg gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg 421 agagcatgtg gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc 481 cgatgcagat ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc 541 catcggcatg caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc 601 gcgcagcgct gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc 661 ctccatgcag atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca 721 tcgtttttca cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat 781 gaccgacacc ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt 841 aggcccgagg gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct 901 ctctgcggcc tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa 961 aaaactcgct gccgtcagtt aataatacta gagctcaagg aggtactaga tgaataatcc 1021 gtcgttactc aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc 1081 ctcaaagtta tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg 1141 ccattgtgac gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt 1201 acaaacgccc gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg 1261 atcgcagatg cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat 1321 cgccccggct tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca 1381 atacagccaa ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt 1441 gatgatggcg caaatcatgg gcgtgcggga taacgccacg ctggaccgcg cctgtgacct 1501 tgggctggca tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg 1561 ccgctgttat ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc 1621 ggcacctgaa aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga 1681 accttactat ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc 1741 aatcgctacg gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca 1801 gcaagcctgg gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc 1861 cgcctctggt caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct 1921 ctggcagcgc ccgctctaat aatactagag ctcaaggagg tactagatga aaccaactac 1981 ggtaattggt gcaggcttcg gtggcctggc actggcaatt cgtctacaag ctgcggggat 2041 ccccgtctta ctgcttgaac aacgtgataa acccggcggt cgggcttatg tctacgagga 2101 tcaggggttt acctttgatg caggcccgac ggttatcacc gatcccagtg ccattgaaga 2161 actgtttgca ctggcaggaa aacagttaaa agagtatgtc gaactgctgc cggttacgcc 2221 gttttaccgc ctgtgttggg agtcagggaa ggtctttaat tacgataacg atcaaacccg 2281 gctcgaagcg cagattcagc agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct 2341 ggactattca cgcgcggtgt ttaaagaagg ctatctaaag ctcggtactg tccctttttt 2401 atcgttcaga gacatgcttc gcgccgcacc tcaactggcg aaactgcaag catggagaag 2461 cgtttacagt aaggttgcca gttacatcga agatgaacat ctgcgccagg cgttttcttt 2521 ccactcgctg ttggtgggcg gcaatccctt cgccacctca tccatttata cgttgataca 2581 cgcgctggag cgtgagtggg gcgtctggtt tccgcgtggc ggcaccggcg cattagttca 2641 ggggatgata aagctgtttc aggatctggg tggcgaagtc gtgttaaacg ccagagtcag 2701 ccatatggaa acgacaggaa acaagattga agccgtgcat ttagaggacg gtcgcaggtt 2761 cctgacgcaa gccgtcgcgt caaatgcaga tgtggttcat acctatcgcg acctgttaag 2821 ccagcaccct gccgcggtta agcagtccaa caaactgcaa actaagcgca tgagtaactc 2881 tctgtttgtg ctctattttg gtttgaatca ccatcatgat cagctcgcgc atcacacggt 2941 ttgtttcggc ccgcgttacc gcgagctgat tgacgaaatt tttaatcatg atggcctcgc 3001 agaggacttc tcactttatc tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga 3061 aggttgcggc agttactatg tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga 3121 ctggacggtt gaggggccaa aactacgcga ccgtattttt gcgtaccttg agcagcatta 3181 catgcctggc ttacggagtc agctggtcac gcaccggatg tttacgccgt ttgattttcg 3241 cgaccagctt aatgcctatc atggctcagc cttttctgtg gagcccgttc ttacccagag 3301 cgcctggttt cggccgcata accgcgataa aaccattact aatctctacc tggtcggcgc 3361 aggcacgcat cccggcgcag gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg 3421 tttgatgctg gaggatctga tataataata ctagagacct gtacgatcct acaggtgctt 3481 atgttaagta attgtattcc cagcgataca atagtgtgac aaaaatccaa tttattagaa 3541 tcaaatgtca atccattacc gttttaatga tatataacac gcaaaacttg cgacaaacaa 3601 taggtaatac tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca 3661 tacagaataa ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta 3721 tctgatatga ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat 3781 tctatggtta aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat 3841 tatgatgacg ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat 3901 tcaccaatta attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta 3961 attaaagaag cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct 4021 aacaatggct tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt 4081 ttatttttac atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat 4141 cgaaaaataa atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa 4201 tgtttagcgt gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc 4261 agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac 4321 cgctgccaaa gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa 4381 aattaataac actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac 4441 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 4501 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 4561 cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa taaa //