##gff-version 3
##sequence-region BBa_S05322 1 4614
BBa_S05322	.	engineered_region	3457	4614	.	+	0	ID=BBa_K546003;Name=BBa_K546003
BBa_S05322	.	engineered_region	3616	4614	.	+	0	ID=BBa_K317037;Name=BBa_K317037;Parent=BBa_K546003
BBa_S05322	.	CDS	3634	4389	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K317037
BBa_S05322	.	point_mutation	4125	4125	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S05322	.	engineered_region	3634	4389	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S05322	.	engineered_region	3634	3635	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S05322	.	CDS	3634	4383	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S05322	.	point_mutation	3807	3807	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S05322	.	engineered_tag	4390	4414	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S05322	.	ribosome_entry_site	3616	3627	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K317037
BBa_S05322	.	conserved_region	3620	3623	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05322	.	promoter	4560	4614	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K317037
BBa_S05322	.	engineered_region	4560	4614	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S05322	.	start_codon	4612	4612	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S05322	.	promoter	4601	4606	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S05322	.	promoter	4579	4584	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S05322	.	non_covalent_binding_site	4560	4579	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S05322	.	terminator	4423	4551	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K317037
BBa_S05322	.	terminator	4511	4551	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S05322	.	stem_loop	4518	4537	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05322	.	stop_codon	4544	4544	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05322	.	polyA_site	4538	4551	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05322	.	engineered_region	4511	4551	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05322	.	terminator	4423	4502	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S05322	.	stem_loop	4434	4477	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05322	.	engineered_region	4423	4502	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05322	.	promoter	3457	3607	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_K546003
BBa_S05322	.	promoter	3545	3550	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S05322	.	promoter	3571	3578	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S05322	.	non_covalent_binding_site	3586	3605	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S05322	.	non_covalent_binding_site	3457	3476	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S05322	.	engineered_region	3457	3607	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S05322	.	start_codon	3584	3584	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S05322	.	engineered_region	1	3448	.	+	0	ID=BBa_S05321;Name=BBa_S05321
BBa_S05322	.	engineered_region	64	3448	.	+	0	ID=BBa_K274100;Name=BBa_K274100;Parent=BBa_S05321
BBa_S05322	.	CDS	1967	3448	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_S05322	.	CDS	1967	3445	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_S05322	.	point_mutation	2444	2449	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_S05322	.	point_mutation	2855	2860	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_S05322	.	ribosome_entry_site	1951	1960	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_S05322	.	sequence_feature	1951	1953	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_S05322	.	ribosome_entry_site	1954	1960	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_S05322	.	CDS	1010	1942	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_S05322	.	CDS	1010	1939	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_S05322	.	ribosome_entry_site	994	1003	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_S05322	.	sequence_feature	994	996	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_S05322	.	ribosome_entry_site	997	1003	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_S05322	.	CDS	74	985	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_S05322	.	ribosome_entry_site	64	67	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
BBa_S05322	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S05321
BBa_S05322	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S05322	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S05322	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S05322	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S05322	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_S05322
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaggtactagatgacg
gtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatc
agttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtat
tcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgt
gcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggac
gccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgt
aattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaa
ggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatc
actttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtga
ttgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacacc
ggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctga
gacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggc
ctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgtta
ctcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaaccc
ggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggc
ccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcg
cagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatc
atctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgt
tgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgacctt
gggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaa
gctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccg
tcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgg
gcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagc
ggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggc
tcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaacca
actacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgc
ttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggt
tatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccg
gttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgc
agattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaagg
ctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaa
gcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgc
tgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctg
gtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgtta
aacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctga
cgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagca
gtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcag
ctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcag
aggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgt
gttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtattttt
gcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttc
gcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgca
taaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatc
ggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataatactagagacctgtacgatcctacaggt
gcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatcca
ttaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttta
cgcaagaaaatggtttgttatagtcgaataaa