LOCUS BBa_S05323 4614 bp DNA linear 26-Dec-2024 DEFINITION K546003:S05321 ACCESSION BBa_S05323 VERSION BBa_S05323.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 terminator 967..1095 /label=BBa_B0015 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 160..1158 /label=BBa_K317037 misc_feature 1..1158 /label=BBa_K546003 misc_feature 1167..1186 /label=LuxR/HSL promoter 1186..1191 /label=-35 promoter 1208..1213 /label=-10 misc_feature 1219..1219 /label=start misc_feature 1167..1221 /label=BBa_R0062 promoter 1167..1221 /label=BBa_R0062 RBS 1230..1233 /label=BBa_K118012 CDS 1240..2151 /label=BBa_I742151 misc_feature 2160..2162 /label=SacI RBS 2163..2169 /label=rbs RBS 2160..2169 /label=BBa_J15001 CDS 2176..3105 /label=crtB coding sequence CDS 2176..3108 /label=BBa_K118002 misc_feature 3117..3119 /label=SacI RBS 3120..3126 /label=rbs RBS 3117..3126 /label=BBa_J15001 misc_feature 3610..3615 /label=PstI site removed misc_feature 4021..4026 /label=PstI site removed CDS 3133..4611 /label=crtI coding sequence CDS 3133..4614 /label=BBa_K118003 misc_feature 1230..4614 /label=BBa_K274100 misc_feature 1167..4614 /label=BBa_S05321 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagacct gtaggatcgt acaggtttac gcaagaaaat 1201 ggtttgttat agtcgaataa atactagagg aggtactaga tgacggtctg cgcaaaaaaa 1261 cacgttcatc tcactcgcga tgctgcggag cagttactgg ctgatattga tcgacgcctt 1321 gatcagttat tgcccgtgga gggagaacgg gatgttgtgg gtgccgcgat gcgtgaaggt 1381 gcgctggcac cgggaaaacg tattcgcccc atgttgctgt tgctgaccgc ccgcgatctg 1441 ggttgcgctg tcagccatga cggattactg gatttggcct gtgcggtgga aatggtccac 1501 gcggcttcgc tgatccttga cgatatgccc tgcatggacg atgcgaagct gcggcgcgga 1561 cgccctacca ttcattctca ttacggagag catgtggcaa tactggcggc ggttgccttg 1621 ctgagtaaag cctttggcgt aattgccgat gcagatggcc tcacgccgct ggcaaaaaat 1681 cgggcggttt ctgaactgtc aaacgccatc ggcatgcaag gattggttca gggtcagttc 1741 aaggatctgt ctgaagggga taagccgcgc agcgctgaag ctattttgat gacgaatcac 1801 tttaaaacca gcacgctgtt ttgtgcctcc atgcagatgg cctcgattgt tgcgaatgcc 1861 tccagcgaag cgcgtgattg cctgcatcgt ttttcacttg atcttggtca ggcatttcaa 1921 ctgctggacg atttgaccga tggcatgacc gacaccggta aggatagcaa tcaggacgcc 1981 ggtaaatcga cgctggtcaa tctgttaggc ccgagggcgg ttgaagaacg tctgagacaa 2041 catcttcagc ttgccagtga gcatctctct gcggcctgcc aacacgggca cgccactcaa 2101 cattttattc aggcctggtt tgacaaaaaa ctcgctgccg tcagttaata atactagagc 2161 tcaaggaggt actagatgaa taatccgtcg ttactcaatc atgcggtcga aacgatggca 2221 gttggctcga aaagttttgc gacagcctca aagttatttg atgcaaaaac ccggcgcagc 2281 gtactgatgc tctacgcctg gtgccgccat tgtgacgatg ttattgacga tcagacgctg 2341 ggctttcagg cccggcagcc tgccttacaa acgcccgaac aacgtctgat gcaacttgag 2401 atgaaaacgc gccaggccta tgcaggatcg cagatgcacg aaccggcgtt tgcggctttt 2461 caggaagtgg ctatggctca tgatatcgcc ccggcttacg cgtttgatca tctggaaggc 2521 ttcgccatgg atgtacgcga agcgcaatac agccaactgg atgatacgct gcgctattgc 2581 tatcacgttg caggcgttgt cggcttgatg atggcgcaaa tcatgggcgt gcgggataac 2641 gccacgctgg accgcgcctg tgaccttggg ctggcatttc agttgaccaa tattgctcgc 2701 gatattgtgg acgatgcgca tgcgggccgc tgttatctgc cggcaagctg gctggagcat 2761 gaaggtctga acaaagagaa ttatgcggca cctgaaaacc gtcaggcgct gagccgtatc 2821 gcccgtcgtt tggtgcagga agcagaacct tactatttgt ctgccacagc cggcctggca 2881 gggttgcccc tgcgttccgc ctgggcaatc gctacggcga agcaggttta ccggaaaata 2941 ggtgtcaaag ttgaacaggc cggtcagcaa gcctgggatc agcggcagtc aacgaccacg 3001 cccgaaaaat taacgctgct gctggccgcc tctggtcagg cccttacttc ccggatgcgg 3061 gctcatcctc cccgccctgc gcatctctgg cagcgcccgc tctaataata ctagagctca 3121 aggaggtact agatgaaacc aactacggta attggtgcag gcttcggtgg cctggcactg 3181 gcaattcgtc tacaagctgc ggggatcccc gtcttactgc ttgaacaacg tgataaaccc 3241 ggcggtcggg cttatgtcta cgaggatcag gggtttacct ttgatgcagg cccgacggtt 3301 atcaccgatc ccagtgccat tgaagaactg tttgcactgg caggaaaaca gttaaaagag 3361 tatgtcgaac tgctgccggt tacgccgttt taccgcctgt gttgggagtc agggaaggtc 3421 tttaattacg ataacgatca aacccggctc gaagcgcaga ttcagcagtt taatccccgc 3481 gatgtcgaag gttatcgtca gtttctggac tattcacgcg cggtgtttaa agaaggctat 3541 ctaaagctcg gtactgtccc ttttttatcg ttcagagaca tgcttcgcgc cgcacctcaa 3601 ctggcgaaac tgcaagcatg gagaagcgtt tacagtaagg ttgccagtta catcgaagat 3661 gaacatctgc gccaggcgtt ttctttccac tcgctgttgg tgggcggcaa tcccttcgcc 3721 acctcatcca tttatacgtt gatacacgcg ctggagcgtg agtggggcgt ctggtttccg 3781 cgtggcggca ccggcgcatt agttcagggg atgataaagc tgtttcagga tctgggtggc 3841 gaagtcgtgt taaacgccag agtcagccat atggaaacga caggaaacaa gattgaagcc 3901 gtgcatttag aggacggtcg caggttcctg acgcaagccg tcgcgtcaaa tgcagatgtg 3961 gttcatacct atcgcgacct gttaagccag caccctgccg cggttaagca gtccaacaaa 4021 ctgcaaacta agcgcatgag taactctctg tttgtgctct attttggttt gaatcaccat 4081 catgatcagc tcgcgcatca cacggtttgt ttcggcccgc gttaccgcga gctgattgac 4141 gaaattttta atcatgatgg cctcgcagag gacttctcac tttatctgca cgcgccctgt 4201 gtcacggatt cgtcactggc gcctgaaggt tgcggcagtt actatgtgtt ggcgccggtg 4261 ccgcatttag gcaccgcgaa cctcgactgg acggttgagg ggccaaaact acgcgaccgt 4321 atttttgcgt accttgagca gcattacatg cctggcttac ggagtcagct ggtcacgcac 4381 cggatgttta cgccgtttga ttttcgcgac cagcttaatg cctatcatgg ctcagccttt 4441 tctgtggagc ccgttcttac ccagagcgcc tggtttcggc cgcataaccg cgataaaacc 4501 attactaatc tctacctggt cggcgcaggc acgcatcccg gcgcaggcat tcctggcgtc 4561 atcggctcgg caaaagcgac agcaggtttg atgctggagg atctgatata ataa //