##gff-version 3
##sequence-region BBa_S05323 1 4614
BBa_S05323	.	engineered_region	1	1158	.	+	0	ID=BBa_K546003;Name=BBa_K546003
BBa_S05323	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_K546003
BBa_S05323	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S05323	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S05323	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S05323	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S05323	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S05323	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S05323	.	engineered_region	160	1158	.	+	0	ID=BBa_K317037;Name=BBa_K317037;Parent=BBa_K546003
BBa_S05323	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K317037
BBa_S05323	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05323	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K317037
BBa_S05323	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S05323	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S05323	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S05323	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S05323	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S05323	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S05323	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K317037
BBa_S05323	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S05323	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S05323	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S05323	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S05323	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S05323	.	terminator	967	1095	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K317037
BBa_S05323	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S05323	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05323	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05323	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S05323	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05323	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05323	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05323	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05323	.	engineered_region	1167	4614	.	+	0	ID=BBa_S05321;Name=BBa_S05321
BBa_S05323	.	engineered_region	1230	4614	.	+	0	ID=BBa_K274100;Name=BBa_K274100;Parent=BBa_S05321
BBa_S05323	.	CDS	2176	3108	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_S05323	.	CDS	2176	3105	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_S05323	.	CDS	1240	2151	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_S05323	.	CDS	3133	4614	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_S05323	.	point_mutation	3610	3615	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_S05323	.	CDS	3133	4611	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_S05323	.	point_mutation	4021	4026	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_S05323	.	ribosome_entry_site	1230	1233	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
BBa_S05323	.	ribosome_entry_site	2160	2169	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_S05323	.	ribosome_entry_site	2163	2169	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_S05323	.	sequence_feature	2160	2162	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_S05323	.	ribosome_entry_site	3117	3126	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_S05323	.	ribosome_entry_site	3120	3126	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_S05323	.	sequence_feature	3117	3119	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_S05323	.	promoter	1167	1221	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S05321
BBa_S05323	.	start_codon	1219	1219	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S05323	.	promoter	1208	1213	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S05323	.	promoter	1186	1191	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S05323	.	engineered_region	1167	1221	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S05323	.	non_covalent_binding_site	1167	1186	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_S05323
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagacctgtaggatcgtacagg
tttacgcaagaaaatggtttgttatagtcgaataaatactagaggaggtactagatgacggtctgcgcaaaaaaacacg
ttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggaggg
agaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttg
ctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacg
cggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctca
ttacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggc
ctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagt
tcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgct
gttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttca
cttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcagg
acgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgc
cagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactc
gctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaa
cgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgct
ctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaa
acgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgt
ttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccat
ggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttg
atgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttga
ccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaagg
tctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagca
gaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagc
aggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcc
cgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcg
catctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcag
gcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacc
cggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgcc
attgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcc
tgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatcc
ccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtact
gtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttaca
gtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcc
cttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcacc
ggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccata
tggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaa
tgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaact
aagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggttt
gtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatct
gcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcat
ttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcatt
acatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgccta
tcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccatt
actaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacag
caggtttgatgctggaggatctgatataataa