BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_S05339
1
BBa_S05339
K880005:K1898400
2016-10-09T11:00:00Z
2016-10-10T09:18:10Z
false
false
_9_
25587
25587
9
false
false
Fiona Tsai
component2490334
1
BBa_K880005
component2490335
1
BBa_K1898400
annotation2490335
1
BBa_K1898400
range2490335
1
62
586
annotation2490334
1
BBa_K880005
range2490334
1
1
55
BBa_K1898400
1
BBa_K1898400
CRYAB, crystallin alpha B
2016-10-09T11:00:00Z
2016-10-19T09:02:13Z
the cDNA or cryab is ordered from OriGene.
This gene codes for cryab, or crystallin alpha B, a crystallin protein exists in the lens and also other tissues and organs.
false
false
_2364_
25587
25587
9
false
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.
false
Fiona Tsai
BBa_K880005
1
BBa_K880005
Strong promoter, strong RBS combination for high expression levels of proteins
2012-09-28T11:00:00Z
2015-05-08T01:13:40Z
It is a combination of strong promoter (J23100) and RBS (B0034).
Released HQ 2013
-J23100+B0034
-Strong promoter, strong RBS combination for high expression levels of proteins
Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
false
false
_1142_
0
9403
9
In stock
false
Enter design considerations.
false
Josh Atkinson, Mike Ferguson, and Ben Parker
component2204230
1
BBa_B0034
component2204228
1
BBa_J23100
annotation2204228
1
BBa_J23100
range2204228
1
1
35
annotation2204230
1
BBa_B0034
range2204230
1
44
55
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_S05339_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggacatcgccatccaccacccctggatccgccgccccttctttcctttccactcccccagccgcctctttgaccagttcttcggagagcacctgttggagtctgatcttttcccgacgtctacttccctgagtcccttctaccttcggccaccctccttcctgcgggcacccagctggtttgacactggactctcagagatgcgcctggagaaggacaggttctctgtcaacctggatgtgaagcacttctccccagaggaactcaaagttaaggtgttgggagatgtgattgaggtgcatggaaaacatgaagagcgccaggatgaacatggtttcatctccagggagttccacaggaaataccggatcccagctgatgtagaccctctcaccattacttcatccctgtcatctgatggggtcctcactgtgaatggaccaaggaaacaggtctctggccctgagcgcaccattcccatcacccgtgaagagaagcctgctgtcaccgcagcccccaagaaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1898400_sequence
1
atggacatcgccatccaccacccctggatccgccgccccttctttcctttccactcccccagccgcctctttgaccagttcttcggagagcacctgttggagtctgatcttttcccgacgtctacttccctgagtcccttctaccttcggccaccctccttcctgcgggcacccagctggtttgacactggactctcagagatgcgcctggagaaggacaggttctctgtcaacctggatgtgaagcacttctccccagaggaactcaaagttaaggtgttgggagatgtgattgaggtgcatggaaaacatgaagagcgccaggatgaacatggtttcatctccagggagttccacaggaaataccggatcccagctgatgtagaccctctcaccattacttcatccctgtcatctgatggggtcctcactgtgaatggaccaaggaaacaggtctctggccctgagcgcaccattcccatcacccgtgaagagaagcctgctgtcaccgcagcccccaagaaa
BBa_K880005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z