BBa_K2170212 1 BBa_K2170212 Red fluorescent protein mRuby3 in RFC[25] 2016-10-10T11:00:00Z 2016-10-11T01:03:41Z genesynthesis mRuby3 false false _2641_ 32573 32573 9 false none false Max Mustermann annotation2493852 1 mRuby3 range2493852 1 1 708 BBa_K2170202 1 BBa_K2170202 StrepTag for interaction with biotin binding poteins in RFC[25] 2016-10-10T11:00:00Z 2016-10-11T08:14:20Z genesynthesis Binding site for biotin binding proteins false false _2641_ 32573 32573 9 false none false Max Mustermann BBa_S05344 1 BBa_S05344 K2170212:K2170202 2016-10-10T11:00:00Z 2016-10-11T07:25:30Z true false _9_ 32573 32573 9 false false Max Mustermann component2491960 1 BBa_K2170202 component2491959 1 BBa_K2170212 annotation2491960 1 BBa_K2170202 range2491960 1 717 740 annotation2491959 1 BBa_K2170212 range2491959 1 1 708 BBa_S05346 1 BBa_S05346 K2170210:S05344 2016-10-10T11:00:00Z 2016-10-11T07:23:41Z true false _9_ 32573 32573 9 false false Max Mustermann component2491966 1 BBa_K2170210 component2491969 1 BBa_S05344 annotation2491966 1 BBa_K2170210 range2491966 1 1 162 annotation2491969 1 BBa_S05344 range2491969 1 171 910 BBa_K2170210 1 BBa_K2170210 Epidermal growth factor receptor transmembrane domain (EGFR TMD) in RFC[25] 2016-09-11T11:00:00Z 2016-10-10T11:28:03Z The part was ordered as genesynthesis from IDT, codonoptimized for ''H. sapiens'' and cloned on pSB1C3. This part encodes for the epidermal growth factor receptor (EGFR) transmembrane domain (TMD). The EGFR stop transfer signal in the 3' region of the TMD increases the membrane stability. The part is ideal for combining internal and external membrane domains to one fusion protein. The glycine linkers in the 5' and 3' region of the part lead to a better flexibility and a better performance of the fused parts. The part is flanked by RFC25 pre- and suffix for further use in proteinfusion. false false _2641_ 32573 32143 9 false . false LMU_TUM_Munich BBa_S05344_sequence 1 gtgagcaaaggtgaagaactgatcaaagaaaacatgcggatgaaagttgttatggaaggtagcgttaatggccaccagtttaaatgtactggtgaaggtgaaggtcgtccgtatgaaggtgttcaggttatgcgtattaaagtgattgaaggtggtccgctgccgtttgcatttgatattctggcaaccagctttatgtatggtagccgtacctttatcaaatatccggcagatattccggacttctttaaacagagctttccggaaggttttacctgggaacgtgttacccgttatgaagatggtggtgttgttaccgttacccaggataccagcctggaagatggcgaactggtttataatgttaaagtgcgtggtgtgaattttccgagcaatggtccggttatgcagaaaaaaaccaaaggttgggaaccgaataccgaaatgatgtatcctgccgatggtggtctgcgtggttataccgatattgcactgaaagttgatggtggcggtcatctgcattgtaattttgttaccacctaccgctcaaaaaaaaccgtgggtaacattaaaatgcctggtgttcatgcagttgatcatcgtctggaacgtattgaagaaagcgataatgaaacctatgttgtgcagcgtgaagttgcagttgcaaaatatagcaatctgggtggtggtatggatgagctgtataaatactagagtggtcccacccccagttcgagaag BBa_K2170210_sequence 1 ggaggcggaggatctggcggaggcggatctccttctatcgccaccggaatggtgggagccctcctgctgctgctggtggtggctctgggcatcggcctgttcatgcggcggagacacatcgtgcggaagagaggcggcggaggaagcggagggggcggatct BBa_S05346_sequence 1 ggaggcggaggatctggcggaggcggatctccttctatcgccaccggaatggtgggagccctcctgctgctgctggtggtggctctgggcatcggcctgttcatgcggcggagacacatcgtgcggaagagaggcggcggaggaagcggagggggcggatcttactagaggtgagcaaaggtgaagaactgatcaaagaaaacatgcggatgaaagttgttatggaaggtagcgttaatggccaccagtttaaatgtactggtgaaggtgaaggtcgtccgtatgaaggtgttcaggttatgcgtattaaagtgattgaaggtggtccgctgccgtttgcatttgatattctggcaaccagctttatgtatggtagccgtacctttatcaaatatccggcagatattccggacttctttaaacagagctttccggaaggttttacctgggaacgtgttacccgttatgaagatggtggtgttgttaccgttacccaggataccagcctggaagatggcgaactggtttataatgttaaagtgcgtggtgtgaattttccgagcaatggtccggttatgcagaaaaaaaccaaaggttgggaaccgaataccgaaatgatgtatcctgccgatggtggtctgcgtggttataccgatattgcactgaaagttgatggtggcggtcatctgcattgtaattttgttaccacctaccgctcaaaaaaaaccgtgggtaacattaaaatgcctggtgttcatgcagttgatcatcgtctggaacgtattgaagaaagcgataatgaaacctatgttgtgcagcgtgaagttgcagttgcaaaatatagcaatctgggtggtggtatggatgagctgtataaatactagagtggtcccacccccagttcgagaag BBa_K2170212_sequence 1 gtgagcaaaggtgaagaactgatcaaagaaaacatgcggatgaaagttgttatggaaggtagcgttaatggccaccagtttaaatgtactggtgaaggtgaaggtcgtccgtatgaaggtgttcaggttatgcgtattaaagtgattgaaggtggtccgctgccgtttgcatttgatattctggcaaccagctttatgtatggtagccgtacctttatcaaatatccggcagatattccggacttctttaaacagagctttccggaaggttttacctgggaacgtgttacccgttatgaagatggtggtgttgttaccgttacccaggataccagcctggaagatggcgaactggtttataatgttaaagtgcgtggtgtgaattttccgagcaatggtccggttatgcagaaaaaaaccaaaggttgggaaccgaataccgaaatgatgtatcctgccgatggtggtctgcgtggttataccgatattgcactgaaagttgatggtggcggtcatctgcattgtaattttgttaccacctaccgctcaaaaaaaaccgtgggtaacattaaaatgcctggtgttcatgcagttgatcatcgtctggaacgtattgaagaaagcgataatgaaacctatgttgtgcagcgtgaagttgcagttgcaaaatatagcaatctgggtggtggtatggatgagctgtataaa BBa_K2170202_sequence 1 tggtcccacccccagttcgagaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z