BBa_S05358 1 BBa_S05358 K1896003:K1896004 2016-10-12T11:00:00Z 2016-10-13T05:56:24Z false false _9_ 19009 19009 9 false false Bob Van Hove component2499904 1 BBa_K1896003 component2499910 1 BBa_K1896004 annotation2499910 1 BBa_K1896004 range2499910 1 925 1344 annotation2499904 1 BBa_K1896003 range2499904 1 1 924 BBa_K1896003 1 BBa_K1896003 INP_NC (opt) 2016-10-11T11:00:00Z 2016-10-18T02:30:04Z [[Part:BBa_K811003|BBa_K811003]] This is an improved version of [[Part:BBa_K811003|BBa_K811003]], which encodes a truncated variant of an ice nucleating protein (INP) found in ''Xanthomonas campestris''. The truncated protein INPNC lacks the large central repeat region, but retains membrane display abilities. The stopcodon and one BsaI site was removed. false false _2362_ 19009 19009 9 false The stop codon was removed to enable use of this part in protein fusions. false Bob Van Hove, Maarten Van Brempt annotation2495421 1 BsaI site removed range2495421 1 660 660 annotation2495419 1 start range2495419 1 1 3 annotation2495420 1 INP_NC range2495420 1 1 924 BBa_K1896004 1 BBa_K1896004 linker-Streptavidin-His (tetrameric) 2016-10-12T11:00:00Z 2016-10-14T05:41:27Z [[Part:BBa_K283010|Part:BBa_K283010]] This is an improved version of [[Part:BBa_K283010|Part:BBa_K283010]], which encodes for the core Streptavidin protein of ''Streptomyces avidinii''. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed. false false _2362_ 19009 19009 9 false One BsaI site was removed to enable Golden Gate cloning. false Bob Van Hove, Maarten Van Brempt annotation2497041 1 BamHI site removed range2497041 1 60 60 annotation2497045 1 stop range2497045 1 415 420 annotation2497040 1 GSTGS linker range2497040 1 1 15 annotation2497042 1 Streptavidin range2497042 1 16 396 annotation2497044 1 His-tag range2497044 1 397 414 BBa_K1896003_sequence 1 atgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggccggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaa BBa_S05358_sequence 1 atgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggccggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa BBa_K1896004_sequence 1 ggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z