BBa_K1896006
1
BBa_K1896006
mGFPuv2 (N-part)
2016-10-12T11:00:00Z
2016-10-14T05:42:05Z
We started from pBAD-GFPuv (GenBank: U62637.1, Clontech), applied the described mutations and codon optimised the sequence for ''E. coli''.
Variant of [[Part:BBa_K1896001|Part:BBa_K1896001]] that lacks stop codons so it can be used in N-terminal protein fusions.
mGFPuv2 contains the following mutations:
* '''F99S/M153T/V163A''': GFPuv or ''cycle 3'' GFP was optimised for excitation by UV light (360-400nm). [1]
* '''S208L''': Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2]
* '''A206K''': Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]
false
false
_2362_
19009
19009
9
false
The stop codon was removed to enable scarless N-terminal protein fusions.
false
Bob Van Hove, Maarten Van Brempt
annotation2499182
1
V163A
range2499182
1
487
489
annotation2499187
1
start
range2499187
1
1
3
annotation2499181
1
M153T
range2499181
1
457
459
annotation2499183
1
A206K
range2499183
1
616
618
annotation2499179
1
mGFPuv2
range2499179
1
1
714
annotation2499180
1
F99S
range2499180
1
295
297
annotation2499184
1
S208L
range2499184
1
622
624
BBa_S05360
1
BBa_S05360
K1896006:K1896004
2016-10-12T11:00:00Z
2016-10-13T05:55:46Z
false
false
_9_
19009
19009
9
false
false
Bob Van Hove
component2499894
1
BBa_K1896006
component2499900
1
BBa_K1896004
annotation2499900
1
BBa_K1896004
range2499900
1
715
1134
annotation2499894
1
BBa_K1896006
range2499894
1
1
714
BBa_K1896004
1
BBa_K1896004
linker-Streptavidin-His (tetrameric)
2016-10-12T11:00:00Z
2016-10-14T05:41:27Z
[[Part:BBa_K283010|Part:BBa_K283010]]
This is an improved version of [[Part:BBa_K283010|Part:BBa_K283010]], which encodes for the core Streptavidin protein of ''Streptomyces avidinii''. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed.
false
false
_2362_
19009
19009
9
false
One BsaI site was removed to enable Golden Gate cloning.
false
Bob Van Hove, Maarten Van Brempt
annotation2497045
1
stop
range2497045
1
415
420
annotation2497044
1
His-tag
range2497044
1
397
414
annotation2497041
1
BamHI site removed
range2497041
1
60
60
annotation2497040
1
GSTGS linker
range2497040
1
1
15
annotation2497042
1
Streptavidin
range2497042
1
16
396
BBa_K1896006_sequence
1
atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaa
BBa_S05360_sequence
1
atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa
BBa_K1896004_sequence
1
ggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z