##gff-version 3
##sequence-region BBa_S05360 1 1134
BBa_S05360	.	CDS	715	1134	.	+	0	ID=BBa_K1896004;Name=BBa_K1896004
BBa_S05360	.	CDS	1111	1128	.	+	0	ID=annotation2497044;Name=His-tag;Parent=BBa_K1896004
BBa_S05360	.	stop_codon	1129	1134	.	+	0	ID=annotation2497045;Name=stop;Parent=BBa_K1896004
BBa_S05360	.	point_mutation	774	774	.	+	0	ID=annotation2497041;Name=BamHI site removed;Parent=BBa_K1896004
BBa_S05360	.	CDS	715	729	.	+	0	ID=annotation2497040;Name=GSTGS linker;Parent=BBa_K1896004
BBa_S05360	.	CDS	730	1110	.	+	0	ID=annotation2497042;Name=Streptavidin;Parent=BBa_K1896004
BBa_S05360	.	CDS	1	714	.	+	0	ID=BBa_K1896006;Name=BBa_K1896006
BBa_S05360	.	sequence_alteration	457	459	.	+	0	ID=annotation2499181;Name=M153T;Parent=BBa_K1896006
BBa_S05360	.	sequence_alteration	487	489	.	+	0	ID=annotation2499182;Name=V163A;Parent=BBa_K1896006
BBa_S05360	.	sequence_alteration	616	618	.	+	0	ID=annotation2499183;Name=A206K;Parent=BBa_K1896006
BBa_S05360	.	start_codon	1	3	.	+	0	ID=annotation2499187;Name=start;Parent=BBa_K1896006
BBa_S05360	.	sequence_alteration	295	297	.	+	0	ID=annotation2499180;Name=F99S;Parent=BBa_K1896006
BBa_S05360	.	CDS	1	714	.	+	0	ID=annotation2499179;Name=mGFPuv2;Parent=BBa_K1896006
BBa_S05360	.	sequence_alteration	622	624	.	+	0	ID=annotation2499184;Name=S208L;Parent=BBa_K1896006
>BBa_S05360
atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaa
cggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaact
acaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaaga
agatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaag
aatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaa
atactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcc
caacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctac
aaaggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccg
ctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcg
ttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaac
gctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacct
ccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgc
ttcccatcaccatcaccaccattaataa