BBa_K1896006 1 BBa_K1896006 mGFPuv2 (N-part) 2016-10-12T11:00:00Z 2016-10-14T05:42:05Z We started from pBAD-GFPuv (GenBank: U62637.1, Clontech), applied the described mutations and codon optimised the sequence for ''E. coli''. Variant of [[Part:BBa_K1896001|Part:BBa_K1896001]] that lacks stop codons so it can be used in N-terminal protein fusions. mGFPuv2 contains the following mutations: * '''F99S/M153T/V163A''': GFPuv or ''cycle 3'' GFP was optimised for excitation by UV light (360-400nm). [1] * '''S208L''': Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2] * '''A206K''': Monomerizes most GFP variants by disrupting a hydrophobic patch. [3] false false _2362_ 19009 19009 9 false The stop codon was removed to enable scarless N-terminal protein fusions. false Bob Van Hove, Maarten Van Brempt annotation2499182 1 V163A range2499182 1 487 489 annotation2499187 1 start range2499187 1 1 3 annotation2499181 1 M153T range2499181 1 457 459 annotation2499183 1 A206K range2499183 1 616 618 annotation2499179 1 mGFPuv2 range2499179 1 1 714 annotation2499180 1 F99S range2499180 1 295 297 annotation2499184 1 S208L range2499184 1 622 624 BBa_S05360 1 BBa_S05360 K1896006:K1896004 2016-10-12T11:00:00Z 2016-10-13T05:55:46Z false false _9_ 19009 19009 9 false false Bob Van Hove component2499894 1 BBa_K1896006 component2499900 1 BBa_K1896004 annotation2499900 1 BBa_K1896004 range2499900 1 715 1134 annotation2499894 1 BBa_K1896006 range2499894 1 1 714 BBa_K1896004 1 BBa_K1896004 linker-Streptavidin-His (tetrameric) 2016-10-12T11:00:00Z 2016-10-14T05:41:27Z [[Part:BBa_K283010|Part:BBa_K283010]] This is an improved version of [[Part:BBa_K283010|Part:BBa_K283010]], which encodes for the core Streptavidin protein of ''Streptomyces avidinii''. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed. false false _2362_ 19009 19009 9 false One BsaI site was removed to enable Golden Gate cloning. false Bob Van Hove, Maarten Van Brempt annotation2497045 1 stop range2497045 1 415 420 annotation2497044 1 His-tag range2497044 1 397 414 annotation2497041 1 BamHI site removed range2497041 1 60 60 annotation2497040 1 GSTGS linker range2497040 1 1 15 annotation2497042 1 Streptavidin range2497042 1 16 396 BBa_K1896006_sequence 1 atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaa BBa_S05360_sequence 1 atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa BBa_K1896004_sequence 1 ggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z