##gff-version 3
##sequence-region BBa_S05361 1 1110
BBa_S05361	.	CDS	1	714	.	+	0	ID=BBa_K1896006;Name=BBa_K1896006
BBa_S05361	.	sequence_alteration	457	459	.	+	0	ID=annotation2499181;Name=M153T;Parent=BBa_K1896006
BBa_S05361	.	sequence_alteration	487	489	.	+	0	ID=annotation2499182;Name=V163A;Parent=BBa_K1896006
BBa_S05361	.	sequence_alteration	616	618	.	+	0	ID=annotation2499183;Name=A206K;Parent=BBa_K1896006
BBa_S05361	.	start_codon	1	3	.	+	0	ID=annotation2499187;Name=start;Parent=BBa_K1896006
BBa_S05361	.	sequence_alteration	295	297	.	+	0	ID=annotation2499180;Name=F99S;Parent=BBa_K1896006
BBa_S05361	.	CDS	1	714	.	+	0	ID=annotation2499179;Name=mGFPuv2;Parent=BBa_K1896006
BBa_S05361	.	sequence_alteration	622	624	.	+	0	ID=annotation2499184;Name=S208L;Parent=BBa_K1896006
BBa_S05361	.	CDS	715	1110	.	+	0	ID=BBa_K1896005;Name=BBa_K1896005
BBa_S05361	.	CDS	715	729	.	+	0	ID=annotation2499130;Name=GSTGS linker;Parent=BBa_K1896005
BBa_S05361	.	CDS	1087	1104	.	+	0	ID=annotation2499132;Name=His-tag;Parent=BBa_K1896005
BBa_S05361	.	stop_codon	1105	1110	.	+	0	ID=annotation2499133;Name=stop;Parent=BBa_K1896005
BBa_S05361	.	CDS	730	1086	.	+	0	ID=annotation2499131;Name=mSA2;Parent=BBa_K1896005
>BBa_S05361
atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaa
cggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaact
acaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaaga
agatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaag
aatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaa
atactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcc
caacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctac
aaaggcagcaccggatctgcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccg
cgggtgcggacggtaacctgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccct
gaccggtcgttacaacggtaccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtacc
gaatggcgtggtcagtaccagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctg
gtccggcgaccgaacagggtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccatta
ataa