BBa_K1896008
1
BBa_K1896008
INP_RC
2016-10-12T11:00:00Z
2016-10-14T05:52:47Z
[[Part:BBa_K1896001|Part:BBa_K1896001]]
INP<sub>RC</sub> is a truncated variant of the ''inaZ'' from ''Pseudomonas syringae'' ([[Part:BBa_K1896001|Part:BBa_K1896001]]), the N-terminal domain was removed in order to prevent localisation to the outer membrane, while still maintaining ice nucleation activity. No stop codon is present in this part to allow seamless assembly of fusion proteins.
false
false
_2362_
19009
19009
9
false
The repeat domain in this part seems to be impossible to create synthetically so PCR was used instead, with primers binding just outside of the repeat region. The absence of a stop codon allows for seamless assembly of fusion proteins.
false
Bob Van Hove, Maarten Van Brempt
annotation2502248
1
repeat region
range2502248
1
22
2949
annotation2502230
1
inaZ_RC
range2502230
1
1
3096
annotation2502231
1
start
range2502231
1
1
3
BBa_S05364
1
BBa_S05364
K1896008:K1896005
2016-10-12T11:00:00Z
2016-10-13T05:54:29Z
false
false
_9_
19009
19009
9
false
false
Bob Van Hove
component2499877
1
BBa_K1896005
component2499872
1
BBa_K1896008
annotation2499877
1
BBa_K1896005
range2499877
1
3097
3492
annotation2499872
1
BBa_K1896008
range2499872
1
1
3096
BBa_K1896005
1
BBa_K1896005
mSA2-His
2016-10-12T11:00:00Z
2016-10-14T05:41:52Z
Combination of streptavidin (''Streptomyces avidinii'') and rhizavidin (''Rhizobium etli''), engineered through homology modeling [1].
Variant of [[Part:BBa_K1896000|Part:BBa_K1896000]] for use in scarless C-terminal protein fusions. This part contains a His-tag instead of a terminal AgeI restriction site.
false
false
_2362_
19009
19009
9
false
This part is not compatible with the Freiburg assembly standard ([[Assembly_standard_25|RFC 25]]).
false
Bob Van Hove, Maarten Van Brempt
annotation2499132
1
His-tag
range2499132
1
373
390
annotation2499130
1
GSTGS linker
range2499130
1
1
15
annotation2499131
1
mSA2
range2499131
1
16
372
annotation2499133
1
stop
range2499133
1
391
396
BBa_K1896008_sequence
1
atgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacggtagtacgcaaaccgcgcaggagcgcagtgatctggtgacaggttatggaagtacctccaccgccggctatgcgagctcgctgattgcgggttatggcagcacgcagactgcgggttatgagagcacgttgaccgccggttacggcagcacgcaaaccgcacaggaaaacagctcgctcaccaccgggtacggaagtacctccacagccggctttgccagctcgctgatctccggttatggcagtacgcagacagccggctataaaagtaccctcacggccggttacggcagtactcagaccgcagagtatggaagctcactcactgcgggctacggcagcactgcaacggccgggcaggacagttcattgatagccggctatggcagctccctgaccagcggaatcagaagttttctgacggcaggctatggcagtacgctgatcgccggacttcgcagcgttttgatcgccggttatggcagtagtcttacatcgggcgttcgcagcacgttgactgcgggttatggcagtaaccagattgcaagttacggcagctcgttgattgcaggccatgaaagcattcaggtcgccggaaataaaagcatgctgatcgccggcaagggcagctcgcagacagcaggttttcgcagcacgctgattgccggtgcgggcagtgtacaactggcgggtgatcgcagccggttgattgccggtgcagacagtaatcagaccgcgggtgaccgcagcaaactgctggccggtaataacagttatctgactgccggcgatagaagcaaactgaccggcgggcatgactgcaccctgatggcgggtgaccaaagcagattgaccgctggtaagaacagtgtcttgacggcaggcgctcgtagcaaactgattggcagtgaaggctcgacgctctcggctggagaagactccatacttattttcaggctctgggacgggaagaggtacaggcaactggtcgccagaacgggtgagaacggtgttgaggccgacataccgtattacgtgaacgaagatgacgatattgtcgataaacccgacgaggacgatgactggatagaggtaaag
BBa_S05364_sequence
1
atgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacggtagtacgcaaaccgcgcaggagcgcagtgatctggtgacaggttatggaagtacctccaccgccggctatgcgagctcgctgattgcgggttatggcagcacgcagactgcgggttatgagagcacgttgaccgccggttacggcagcacgcaaaccgcacaggaaaacagctcgctcaccaccgggtacggaagtacctccacagccggctttgccagctcgctgatctccggttatggcagtacgcagacagccggctataaaagtaccctcacggccggttacggcagtactcagaccgcagagtatggaagctcactcactgcgggctacggcagcactgcaacggccgggcaggacagttcattgatagccggctatggcagctccctgaccagcggaatcagaagttttctgacggcaggctatggcagtacgctgatcgccggacttcgcagcgttttgatcgccggttatggcagtagtcttacatcgggcgttcgcagcacgttgactgcgggttatggcagtaaccagattgcaagttacggcagctcgttgattgcaggccatgaaagcattcaggtcgccggaaataaaagcatgctgatcgccggcaagggcagctcgcagacagcaggttttcgcagcacgctgattgccggtgcgggcagtgtacaactggcgggtgatcgcagccggttgattgccggtgcagacagtaatcagaccgcgggtgaccgcagcaaactgctggccggtaataacagttatctgactgccggcgatagaagcaaactgaccggcgggcatgactgcaccctgatggcgggtgaccaaagcagattgaccgctggtaagaacagtgtcttgacggcaggcgctcgtagcaaactgattggcagtgaaggctcgacgctctcggctggagaagactccatacttattttcaggctctgggacgggaagaggtacaggcaactggtcgccagaacgggtgagaacggtgttgaggccgacataccgtattacgtgaacgaagatgacgatattgtcgataaacccgacgaggacgatgactggatagaggtaaagggcagcaccggatctgcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccgcgggtgcggacggtaacctgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccctgaccggtcgttacaacggtaccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtaccgaatggcgtggtcagtaccagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctggtccggcgaccgaacagggtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccattaataa
BBa_K1896005_sequence
1
ggcagcaccggatctgcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccgcgggtgcggacggtaacctgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccctgaccggtcgttacaacggtaccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtaccgaatggcgtggtcagtaccagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctggtccggcgaccgaacagggtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z