LOCUS BBa_S05372 2249 bp DNA linear 05-Nov-2024 DEFINITION S05371:R0010 ACCESSION BBa_S05372 VERSION BBa_S05372.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..717 /label=mTagBFP CDS 19..723 /label=BBa_K592100 misc_feature 1..723 /label=BBa_K592024 misc_feature 738..741 /label=conserved RBS 732..745 /label=BBa_B0031 misc_feature 1841..1879 /label=LVA CDS 752..1879 /label=lacI-LVA misc_feature 752..1879 /label=BBa_C0012 misc_feature 1880..1904 /label=Help:Barcodes CDS 752..1879 /label=BBa_C0012 misc_feature 732..1904 /label=BBa_I14044 misc_feature 1..1904 /label=BBa_S05370 stem_loop 1924..1967 /label=stem_loop misc_feature 1913..1992 /label=BBa_B0010 terminator 1913..1992 /label=BBa_B0010 stem_loop 2008..2027 /label=T7 TE misc_feature 2034..2034 /label=stop polyA_site 2028..2041 /label=polya misc_feature 2001..2041 /label=BBa_B0012 terminator 2001..2041 /label=BBa_B0012 terminator 1913..2041 /label=BBa_B0015 misc_feature 1..2041 /label=BBa_S05371 CDS 2050..2137 /label=end of LacI coding region (inactive) misc_feature 2050..2249 /label=BBa_R0010 misc_feature 2138..2175 /label=CAP binding site promoter 2186..2191 /label=-35 promoter 2210..2215 /label=-10 misc_feature 2215..2249 /label=LacI binding site misc_feature 2222..2222 /label=start promoter 2050..2249 /label=BBa_R0010 ORIGIN 1 aaagaggaga aatactagat gagcgaactg atcaaagaga acatgcacat gaagctgtac 61 atggaaggca ccgttgacaa ccaccacttt aagtgcacgt ctgagggtga gggtaagccg 121 tacgaaggca cccaaaccat gcgtatcaaa gttgtggagg gcggtccact gccgttcgct 181 tttgacattc tggcgaccag cttcctgtac ggttccaaaa cgttcattaa ccatactcag 241 ggcattccgg atttcttcaa acagagcttt ccggaaggtt tcacctggga gcgtgtcacc 301 acgtatgaag atggtggtgt gttgaccgcc acccaagata cctccctgca agatggctgt 361 ctgatctata acgtgaaaat tcgtggcgtc aactttacga gcaatggtcc ggtgatgcag 421 aagaaaaccc tgggttggga ggcgtttacg gaaaccctgt atccggccga tggtggcctg 481 gagggccgta acgacatggc actgaagctg gttggtggca gccatttgat cgcaaatatc 541 aagacgacgt accgcagcaa gaaaccggcg aaaaatctga agatgccggg tgtttactat 601 gtcgactacc gtctggaacg cattaaagaa gcgaataatg agacttacgt ggagcagcac 661 gaggttgcag tcgcgcgcta ttgcgacttg cctagcaagc tgggtcataa actgaattaa 721 taatactaga gtcacacagg aaacctacta gatggtgaat gtgaaaccag taacgttata 781 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 841 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 901 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 961 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1021 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1081 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1141 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1201 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1261 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1321 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1381 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1441 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1501 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1561 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1621 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1681 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1741 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1801 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 1861 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 1921 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1981 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2041 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 2101 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 2161 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 2221 gaattgtgag cggataacaa tttcacaca //