LOCUS       BBa_S05372              2249 bp    DNA     linear       26-Nov-2024
DEFINITION  S05371:R0010
ACCESSION   BBa_S05372
VERSION     BBa_S05372.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..717
                     /label=mTagBFP
     CDS             19..723
                     /label=BBa_K592100
     misc_feature    1..723
                     /label=BBa_K592024
     misc_feature    738..741
                     /label=conserved
     RBS             732..745
                     /label=BBa_B0031
     misc_feature    1841..1879
                     /label=LVA
     CDS             752..1879
                     /label=lacI-LVA
     misc_feature    752..1879
                     /label=BBa_C0012
     misc_feature    1880..1904
                     /label=Help:Barcodes
     CDS             752..1879
                     /label=BBa_C0012
     misc_feature    732..1904
                     /label=BBa_I14044
     misc_feature    1..1904
                     /label=BBa_S05370
     stem_loop       1924..1967
                     /label=stem_loop
     misc_feature    1913..1992
                     /label=BBa_B0010
     terminator      1913..1992
                     /label=BBa_B0010
     stem_loop       2008..2027
                     /label=T7 TE
     misc_feature    2034..2034
                     /label=stop
     polyA_site      2028..2041
                     /label=polya
     misc_feature    2001..2041
                     /label=BBa_B0012
     terminator      2001..2041
                     /label=BBa_B0012
     terminator      1913..2041
                     /label=BBa_B0015
     misc_feature    1..2041
                     /label=BBa_S05371
     CDS             2050..2137
                     /label=end of LacI coding region (inactive)
     misc_feature    2050..2249
                     /label=BBa_R0010
     misc_feature    2138..2175
                     /label=CAP binding site
     promoter        2186..2191
                     /label=-35
     promoter        2210..2215
                     /label=-10
     misc_feature    2215..2249
                     /label=LacI binding site
     misc_feature    2222..2222
                     /label=start
     promoter        2050..2249
                     /label=BBa_R0010
ORIGIN
        1 aaagaggaga aatactagat gagcgaactg atcaaagaga acatgcacat gaagctgtac
       61 atggaaggca ccgttgacaa ccaccacttt aagtgcacgt ctgagggtga gggtaagccg
      121 tacgaaggca cccaaaccat gcgtatcaaa gttgtggagg gcggtccact gccgttcgct
      181 tttgacattc tggcgaccag cttcctgtac ggttccaaaa cgttcattaa ccatactcag
      241 ggcattccgg atttcttcaa acagagcttt ccggaaggtt tcacctggga gcgtgtcacc
      301 acgtatgaag atggtggtgt gttgaccgcc acccaagata cctccctgca agatggctgt
      361 ctgatctata acgtgaaaat tcgtggcgtc aactttacga gcaatggtcc ggtgatgcag
      421 aagaaaaccc tgggttggga ggcgtttacg gaaaccctgt atccggccga tggtggcctg
      481 gagggccgta acgacatggc actgaagctg gttggtggca gccatttgat cgcaaatatc
      541 aagacgacgt accgcagcaa gaaaccggcg aaaaatctga agatgccggg tgtttactat
      601 gtcgactacc gtctggaacg cattaaagaa gcgaataatg agacttacgt ggagcagcac
      661 gaggttgcag tcgcgcgcta ttgcgacttg cctagcaagc tgggtcataa actgaattaa
      721 taatactaga gtcacacagg aaacctacta gatggtgaat gtgaaaccag taacgttata
      781 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      841 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      901 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      961 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     1021 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     1081 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     1141 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     1201 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     1261 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     1321 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     1381 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     1441 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     1501 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     1561 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     1621 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     1681 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     1741 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1801 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta
     1861 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat
     1921 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1981 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2041 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2101 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2161 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2221 gaattgtgag cggataacaa tttcacaca
//