##gff-version 3
##sequence-region BBa_S05372 1 2249
BBa_S05372	.	engineered_region	1	2041	.	+	0	ID=BBa_S05371;Name=BBa_S05371
BBa_S05372	.	engineered_region	1	1904	.	+	0	ID=BBa_S05370;Name=BBa_S05370;Parent=BBa_S05371
BBa_S05372	.	engineered_region	1	723	.	+	0	ID=BBa_K592024;Name=BBa_K592024;Parent=BBa_S05370
BBa_S05372	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592024
BBa_S05372	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05372	.	CDS	19	723	.	+	0	ID=BBa_K592100;Name=BBa_K592100;Parent=BBa_K592024
BBa_S05372	.	CDS	19	717	.	+	0	ID=annotation2135490;Name=mTagBFP;Parent=BBa_K592100
BBa_S05372	.	engineered_region	732	1904	.	+	0	ID=BBa_I14044;Name=BBa_I14044;Parent=BBa_S05370
BBa_S05372	.	ribosome_entry_site	732	745	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_I14044
BBa_S05372	.	conserved_region	738	741	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_S05372	.	CDS	752	1879	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I14044
BBa_S05372	.	engineered_tag	1880	1904	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S05372	.	CDS	752	1879	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S05372	.	engineered_region	752	1879	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S05372	.	tag	1841	1879	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S05372	.	terminator	1913	2041	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05371
BBa_S05372	.	terminator	2001	2041	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S05372	.	stem_loop	2008	2027	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05372	.	polyA_site	2028	2041	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05372	.	stop_codon	2034	2034	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05372	.	engineered_region	2001	2041	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05372	.	terminator	1913	1992	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S05372	.	stem_loop	1924	1967	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05372	.	engineered_region	1913	1992	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05372	.	promoter	2050	2249	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S05372	.	CDS	2050	2137	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S05372	.	promoter	2210	2215	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S05372	.	non_covalent_binding_site	2215	2249	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S05372	.	engineered_region	2050	2249	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S05372	.	promoter	2186	2191	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S05372	.	non_covalent_binding_site	2138	2175	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S05372	.	start_codon	2222	2222	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_S05372
aaagaggagaaatactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgaca
accaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtgga
gggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatact
cagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtg
gtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactt
tacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggt
ggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtacc
gcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagc
gaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaa
ctgaattaataatactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgca
gagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaa
aagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgct
gattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaa
ctgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcg
cgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaa
tgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcga
ctgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgc
gtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggag
tgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgat
cagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacg
acgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccag
cgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaaga
aaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
gtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat
gttgtgtggaattgtgagcggataacaatttcacaca