BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961224
1
-35
range1961224
1
137
142
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508159
1
BBa_B0034
component1508149
1
BBa_R0010
annotation1508159
1
BBa_B0034
range1508159
1
209
220
annotation1508149
1
BBa_R0010
range1508149
1
1
200
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K902054
1
BBa_K902054
Inducible DszB generator (J04500-DszB)
2012-09-30T11:00:00Z
2015-05-08T01:13:43Z
DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry.
Inducible expression (using LacI promotor J04500) of DszB
false
false
_1167_
0
13399
9
In stock
false
See DszB mutations
false
Lisa Oberding, Ali Honarmand
component2205166
1
BBa_K902052
component2205160
1
BBa_J04500
annotation2205160
1
BBa_J04500
range2205160
1
1
220
annotation2205166
1
BBa_K902052
range2205166
1
227
1324
BBa_S05378
1
BBa_S05378
K902054:K804005
2016-12-09T12:00:00Z
2016-12-09T12:06:51Z
false
false
_9_
24251
24251
9
false
false
Gilles Yvan Buck
component2531799
1
BBa_K804005
component2531798
1
BBa_K902054
annotation2531798
1
BBa_K902054
range2531798
1
1
1324
annotation2531799
1
BBa_K804005
range2531799
1
1331
2584
BBa_K902052
1
dszB
HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)
2012-09-30T11:00:00Z
2015-05-08T01:13:43Z
Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.
DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.
false
false
_1167_
0
13399
9
In stock
false
Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.
false
Lisa Oberding, Ali Honarmand
annotation2205136
1
G->A
range2205136
1
1026
1026
annotation2205133
1
start
range2205133
1
1
3
annotation2211179
1
stop
range2211179
1
1096
1098
annotation2205134
1
Y63F
range2205134
1
187
189
annotation2205135
1
G->A
range2205135
1
339
339
annotation2205132
1
DszB
range2205132
1
1
1098
BBa_K804005
1
BBa_K804005
DszC coding gene (from DszABC operon)
2012-09-30T11:00:00Z
2015-05-08T01:13:25Z
http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)
This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases.
false
false
_1060_
0
9774
9
It's complicated
false
2 PstI sites were removed using site-directed mutagenesis
false
Joe Ho
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K902054_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_S05378_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
BBa_K902052_sequence
1
atgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
BBa_K804005_sequence
1
atgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z