LOCUS BBa_S05385 4072 bp DNA linear 05-Nov-2024 DEFINITION S05382:B0053 ACCESSION BBa_S05385 VERSION BBa_S05385.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..1324 /label=DszB misc_feature 227..229 /label=start misc_feature 413..415 /label=Y63F misc_feature 565..565 /label=G->A misc_feature 1252..1252 /label=G->A misc_feature 1322..1324 /label=stop CDS 227..1324 /label=BBa_K902052 misc_feature 1..1324 /label=BBa_K902054 misc_feature 1337..1340 /label=conserved RBS 1333..1344 /label=BBa_B0034 misc_feature 1..1344 /label=BBa_S05379 CDS 1351..2604 /label=BBa_K804005 misc_feature 1..2604 /label=BBa_S05380 misc_feature 2617..2620 /label=conserved RBS 2613..2624 /label=BBa_B0034 misc_feature 1..2624 /label=BBa_S05381 misc_feature 2631..2633 /label=start misc_feature 3269..3269 /label=G->A misc_feature 3308..3308 /label=G->A misc_feature 3641..3641 /label=G->A misc_feature 3896..3896 /label=G->A CDS 2631..3992 /label=DszA misc_feature 3990..3992 /label=stop CDS 2631..3992 /label=BBa_K902050 misc_feature 1..3992 /label=BBa_S05382 stem_loop 4009..4043 /label=stem_loop terminator 4001..4072 /label=BBa_B0053 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga caagccgcgt 241 cgaccccgca aaccccggtt cagaactcga ttccgccatc cgcgacacac tgacctacag 301 caactgcccg gtacccaacg ctctgctcac ggcatcggaa tcgggcttcc tcgacgccgc 361 cggcatcgaa ctcgacgtcc tcagcggcca gcagggcacg gttcatttca ccttcgacca 421 gcctgcctac acccgttttg ggggtgagat cccgccactg ctcagcgagg ggttgcgggc 481 acctgggcgc acgcgtctac tcggcatcac cccgctcttg gggcgccagg gcttctttgt 541 ccgcgacgac agcccgatca cagcagccgc cgaccttgcc ggacgtcgaa tcggcgtctc 601 ggcctcggca attcgcatcc tgcgcggcca gctgggcgac tacctcgagt tggatccctg 661 gcggcaaacg ctggtagcgc tgggctcgtg ggaggcgcgc gccttgttgc acacccttga 721 gcacggtgaa ctgggtgtgg acgacgtcga gctggtgccg atcagcagtc ctggtgtcga 781 tgttcccgct gagcagctcg aagaatcggc gaccgtcaag ggtgcggacc tctttcccga 841 tgtcgcccgc ggtcaggccg cggtgttggc cagcggagac gttgacgccc tgtacagttg 901 gctgccctgg gccggggagt tgcaagccac cggggcccgc ccagtggtgg atctcggcct 961 cgatgagcgc aatgcctacg ccagtgtgtg gacggtcagc agcgggctgg ttcgccagcg 1021 acctggcctt gttcaacgac tggtcgacgc ggccgtcgac gccgggctgt gggcacgcga 1081 tcattccgac gcggtgacca gcctgcacgc cgcgaacctg ggcgtatcga ccggagcagt 1141 aggccagggc ttcggcgccg acttccagca gcgtctggtt ccacgcctgg atcacgacgc 1201 cctcgccctc ctggagcgca cacagcaatt cctgctcacc aacaacttgc tacaggaacc 1261 cgtcgccctc gatcagtggg cggctccgga atttctgaac aacagcctca atcgccaccg 1321 atagtactag agaaagagga gaaatactag atgacactgt cacctgaaaa gcagcacgtt 1381 cgaccacgcg acgccgccga caacgatccc gtcgcggttg cccgtgggct agccgaaaag 1441 tggcgagcca ccgccgtcga gcgtgatcgc gccgggggtt cggcaacagc cgagcgcgaa 1501 gacctgcgcg cgagcgcgct gctgtcgctc ctcgtcccgc gcgaatacgg cggctggggc 1561 gcagactggc ccaccgccat cgaggtcgtc cgcgaaatcg cggcagccga tggatctttg 1621 ggacacctgt tcggatacca cctcaccaac gccccgatga tcgaactgat cggctcgcag 1681 gaacaagaag aacacctgta cacccagatc gcgcagaaca actggtggac cggaaatgcc 1741 tccagcgaga acaacagcca cgtgctggac tggaaggtca gcgccacccc gaccgaagac 1801 ggcggctacg tgctcaatgg cacgaagcac ttctacagcg gcgccaaggg gtcggacctg 1861 ctgttcgtgt tcggcgtcgt ccaggatgat tctccgcagc agggtgcgat cattgctgcc 1921 gctatcccga catcgcgggc tggcgttacg cccaacgacg actgggccgc catcggcatg 1981 cggcagaccg acagcggttc cacggacttc cacaacgtca aggtcgagcc tgacgaagtg 2041 ctgggcgcgc ccaacgcctt cgttctcgcc ttcatacaat ccgagcgcgg cagcctcttc 2101 cggcccatag cgcaattgat cttcgccaac gtctatctgg ggatcgcgca cggcgcactc 2161 gatgccgcca gggagtacac ccgtacccag gcgaggccct ggacaccggc cggtattcaa 2221 caggcaaccg aggatcccta caccatccgc tcctacggtg agttcaccat cgcattgcag 2281 ggagctgacg ccgccgcccg tgaagcggcc cacctgctac agacggtgtg ggacaagggc 2341 gacgcgctca cccccgagga ccgcggcgaa ctgatggtga aggtctcggg agtcaaagcg 2401 ttggccacca acgccgccct caacatcagc agcggcgtct tcgaggtgat cggcgcgcgc 2461 ggaacacatc ccaggtacgg tttcgaccgc ttctggcgca acgtgcgcac ccactccctg 2521 cacgacccgg tgtcctacaa gatcgccgac gtcggcaagc acaccttgaa cggtcaatac 2581 ccgattcccg gcttcacctc ctgatactag agaaagagga gaaatactag atgactcaac 2641 aacgacaaat gcatctggcc ggtttcttct cggccggcaa tgtgactcat gcacatgggg 2701 cgtggcggca cacggacgcg tcgaatgact ttctgtcggg gaagtactac caacacatcg 2761 cccgtactct ggagcgcggc aagttcgatc tgttgtttct gcctgacggg ttggccgtcg 2821 aggacagcta cggggacaac ctggacaccg gtgtcggcct gggcgggcag ggtgcagtcg 2881 ccttggagcc ggccagtgtg gtcgcaacca tggccgcggt gaccgagcac ctgggtcttg 2941 gggcaaccat ttcggcgacc tactatcccc cgtatcacgt tgctcgggtg ttcgcgacgc 3001 tcgatcagtt gtcagggggt cgggtgtcct ggaacgtcgt cacctcgctc aacgacgctg 3061 aagcgcgcaa cttcggcatt aatcagcatc tggaacacga cgcccgctat gaccgcgccg 3121 atgagttctt ggaagcggtc aagaaactct ggaacagctg ggacgaggac gccctcgtgc 3181 tggacaaggc ggccggcgtg ttcgccgatc ccgcgaaggt gcactacgtc gatcaccacg 3241 gggagtggct gaatgtgcgc ggacctctac aggtaccgcg ttcacctcag ggtgagccgg 3301 tgatcctaca ggccggcctg tcgccccggg gtcggcgctt cgccgggaag tgggccgagg 3361 ccgtcttcag tcttgcaccc aacctcgagg tgatgcaggc cacctaccag ggcatcaaag 3421 ccgaggtcga cgctgcgggg cgcgatcccg atcagacgaa aatcttcacc gccgtgatgc 3481 cggtactcgg cgaaagccag gcggtggcac aggaacgact ggaatatctc aacagtctgg 3541 tccatccgga agtgggactg tcgacgctat ccagtcacac cggcatcaac ctggcggcgt 3601 accctctcga cactccgatc aaggacatcc tgcgggatct acaggatcgg aatgtcccga 3661 cgcaactgca catgttcgcc gccgcaacgc acagcgaaga gctcacgctg gcggaaatgg 3721 gtcggcgcta tggaaccaac gtggggttcg ttcctcagtg ggccggtacc ggggagcaga 3781 tcgctgacga gctgatccgc cacttcgagg gcggcgccgc ggatggtttc atcatctctc 3841 cggccttcct gccgggctcc tacgacgagt tcgtcgacca ggtggttccg gttctacagg 3901 atcgcggcta cttccgcacc gagtaccagg gcaacactct gcgcgaccac ttgggtctgc 3961 gcgtaccaca actgcaagga caaccttcat gatactagag tccggcaaaa aaacgggcaa 4021 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tt //