##gff-version 3
##sequence-region BBa_S05385 1 4072
BBa_S05385	.	terminator	4001	4072	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_S05385	.	stem_loop	4009	4043	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_S05385	.	engineered_region	1	3992	.	+	0	ID=BBa_S05382;Name=BBa_S05382
BBa_S05385	.	engineered_region	1	2624	.	+	0	ID=BBa_S05381;Name=BBa_S05381;Parent=BBa_S05382
BBa_S05385	.	ribosome_entry_site	2613	2624	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05381
BBa_S05385	.	conserved_region	2617	2620	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05385	.	engineered_region	1	2604	.	+	0	ID=BBa_S05380;Name=BBa_S05380;Parent=BBa_S05381
BBa_S05385	.	engineered_region	1	1344	.	+	0	ID=BBa_S05379;Name=BBa_S05379;Parent=BBa_S05380
BBa_S05385	.	ribosome_entry_site	1333	1344	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05379
BBa_S05385	.	conserved_region	1337	1340	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05385	.	engineered_region	1	1324	.	+	0	ID=BBa_K902054;Name=BBa_K902054;Parent=BBa_S05379
BBa_S05385	.	CDS	227	1324	.	+	0	ID=BBa_K902052;Name=BBa_K902052;Parent=BBa_K902054
BBa_S05385	.	sequence_alteration	1252	1252	.	+	0	ID=annotation2205136;Name=G->A;Parent=BBa_K902052
BBa_S05385	.	stop_codon	1322	1324	.	+	0	ID=annotation2211179;Name=stop;Parent=BBa_K902052
BBa_S05385	.	CDS	227	1324	.	+	0	ID=annotation2205132;Name=DszB;Parent=BBa_K902052
BBa_S05385	.	sequence_alteration	413	415	.	+	0	ID=annotation2205134;Name=Y63F;Parent=BBa_K902052
BBa_S05385	.	start_codon	227	229	.	+	0	ID=annotation2205133;Name=start;Parent=BBa_K902052
BBa_S05385	.	sequence_alteration	565	565	.	+	0	ID=annotation2205135;Name=G->A;Parent=BBa_K902052
BBa_S05385	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K902054
BBa_S05385	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_S05385	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S05385	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S05385	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S05385	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S05385	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S05385	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S05385	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S05385	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_S05385	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05385	.	CDS	1351	2604	.	+	0	ID=BBa_K804005;Name=BBa_K804005;Parent=BBa_S05380
BBa_S05385	.	CDS	2631	3992	.	+	0	ID=BBa_K902050;Name=BBa_K902050;Parent=BBa_S05382
BBa_S05385	.	CDS	2631	3992	.	+	0	ID=annotation2205093;Name=DszA;Parent=BBa_K902050
BBa_S05385	.	sequence_alteration	3269	3269	.	+	0	ID=annotation2205052;Name=G->A;Parent=BBa_K902050
BBa_S05385	.	start_codon	2631	2633	.	+	0	ID=annotation2205051;Name=start;Parent=BBa_K902050
BBa_S05385	.	sequence_alteration	3308	3308	.	+	0	ID=annotation2205053;Name=G->A;Parent=BBa_K902050
BBa_S05385	.	sequence_alteration	3641	3641	.	+	0	ID=annotation2205054;Name=G->A;Parent=BBa_K902050
BBa_S05385	.	stop_codon	3990	3992	.	+	0	ID=annotation2211180;Name=stop;Parent=BBa_K902050
BBa_S05385	.	sequence_alteration	3896	3896	.	+	0	ID=annotation2205055;Name=G->A;Parent=BBa_K902050
>BBa_S05385
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccg
cgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtaccc
aacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagg
gcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggtt
gcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagc
ccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagc
tgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgca
cacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgct
gagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttgg
ccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggt
ggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggc
cttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgc
acgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacg
cctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtc
gccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaa
atactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcc
cgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaag
acctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccat
cgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatg
atcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatg
cctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaa
tggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccg
cagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcg
gcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaa
cgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtc
tatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccg
gtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgc
cgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaa
ctgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtga
tcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgaccc
ggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctgatac
tagagaaagaggagaaatactagatgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgac
tcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgt
actctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctgg
acaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgac
cgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctc
gatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcatta
atcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctg
ggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccac
ggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcc
tgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgca
ggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtg
atgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgg
gactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcg
ggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcg
gaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagc
tgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagtt
cgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccac
ttgggtctgcgcgtaccacaactgcaaggacaaccttcatgatactagagtccggcaaaaaaacgggcaaggtgtcacc
accctgccctttttctttaaaaccgaaaagattacttcgcgtt