BBa_S05389
type: ComponentDefinition
persistent identity: BBa S05389
display id: BBa_S05389
version: 1
was generated by: iGEM to SBOL conversion
description: S05388:K902057
created: 2016-12-09T12:00:00Z
modified: 2016-12-09T01:04:38Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S05388
component: BBa_K902057
sequence annotation: BBa_S05388
sequence annotation: BBa_K902057
sequence: https://synbiohub.org/public/igem/BBa_S05389_sequence/1

BBa_S05388
type: Component
persistent identity: component2532236
display id: component2532236
version: 1
top level: BBa_S05389
definition: BBa_S05388
access: public

BBa_K902057
type: Component
persistent identity: component2532240
display id: component2532240
version: 1
top level: BBa_S05389
definition: hpaC
access: public

BBa_S05388
type: SequenceAnnotation
persistent identity: annotation2532236
display id: annotation2532236
version: 1
top level: BBa_S05389
location: https://synbiohub.org/public/igem/BBa_S05389/annotation2532236/range2532236/1
component: BBa_S05388

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type: Range
persistent identity: range2532236
display id: range2532236
version: 1
top level: BBa_S05389
direction: direction/reverse
start: 1
end: 4012
orientation: inline

BBa_K902057
type: SequenceAnnotation
persistent identity: annotation2532240
display id: annotation2532240
version: 1
top level: BBa_S05389
location: https://synbiohub.org/public/igem/BBa_S05389/annotation2532240/range2532240/1
component: BBa_K902057

https://synbiohub.org/public/igem/BBa_S05389/annotation2532240/range2532240/1
type: Range
persistent identity: range2532240
display id: range2532240
version: 1
top level: BBa_S05389
direction: direction/reverse
start: 4019
end: 4531
orientation: inline

hpaC
type: ComponentDefinition
persistent identity: BBa K902057
display id: BBa_K902057
version: 1
was generated by: iGEM to SBOL conversion
description: HpaC- 4-hydroxyphenylacetate 3-monooxygenase reductase subunit
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Escherichia coli W
owned by: james
owned by: myers
mutable description: Recycles FMNH2 utilizing NADH and FMN.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: HpaC
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902057_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2205091
display id: annotation2205091
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2205091/range2205091/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2205091
display id: range2205091
version: 1
top level: hpaC
direction: direction/forward
start: 1
end: 3
orientation: inline

HpaC
type: SequenceAnnotation
persistent identity: annotation2205092
display id: annotation2205092
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2205092/range2205092/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2205092
display id: range2205092
version: 1
top level: hpaC
direction: direction/forward
start: 1
end: 513
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211176
display id: annotation2211176
version: 1
top level: hpaC
location: https://synbiohub.org/public/igem/BBa_K902057/annotation2211176/range2211176/1
role: feature/stop
role: SO:0000319

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type: Range
persistent identity: range2211176
display id: range2211176
version: 1
top level: hpaC
direction: direction/forward
start: 511
end: 513
orientation: inline

BBa_S05388
type: ComponentDefinition
persistent identity: BBa S05388
display id: BBa_S05388
version: 1
was generated by: iGEM to SBOL conversion
description: S05382:B0034
created: 2016-12-09T12:00:00Z
modified: 2016-12-09T01:03:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_S05382
sequence annotation: BBa_S05382
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S05388_sequence/1

BBa_B0034
type: Component
persistent identity: component2532200
display id: component2532200
version: 1
top level: BBa_S05388
definition: BBa_B0034
access: public

BBa_S05382
type: Component
persistent identity: component2532198
display id: component2532198
version: 1
top level: BBa_S05388
definition: BBa_S05382
access: public

BBa_S05382
type: SequenceAnnotation
persistent identity: annotation2532198
display id: annotation2532198
version: 1
top level: BBa_S05388
location: https://synbiohub.org/public/igem/BBa_S05388/annotation2532198/range2532198/1
component: BBa_S05382

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type: Range
persistent identity: range2532198
display id: range2532198
version: 1
top level: BBa_S05388
direction: direction/reverse
start: 1
end: 3992
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2532200
display id: annotation2532200
version: 1
top level: BBa_S05388
location: https://synbiohub.org/public/igem/BBa_S05388/annotation2532200/range2532200/1
component: BBa_B0034

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type: Range
persistent identity: range2532200
display id: range2532200
version: 1
top level: BBa_S05388
direction: direction/reverse
start: 4001
end: 4012
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_S05382
type: ComponentDefinition
persistent identity: BBa S05382
display id: BBa_S05382
version: 1
was generated by: iGEM to SBOL conversion
description: S05381:K902050
created: 2016-12-09T12:00:00Z
modified: 2016-12-09T12:11:02Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S05381
component: BBa_K902050
sequence annotation: BBa_S05381
sequence annotation: BBa_K902050
sequence: https://synbiohub.org/public/igem/BBa_S05382_sequence/1

BBa_S05381
type: Component
persistent identity: component2531884
display id: component2531884
version: 1
top level: BBa_S05382
definition: BBa_S05381
access: public

BBa_K902050
type: Component
persistent identity: component2531892
display id: component2531892
version: 1
top level: BBa_S05382
definition: dszA
access: public

BBa_S05381
type: SequenceAnnotation
persistent identity: annotation2531884
display id: annotation2531884
version: 1
top level: BBa_S05382
location: https://synbiohub.org/public/igem/BBa_S05382/annotation2531884/range2531884/1
component: BBa_S05381

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type: Range
persistent identity: range2531884
display id: range2531884
version: 1
top level: BBa_S05382
direction: direction/reverse
start: 1
end: 2624
orientation: inline

BBa_K902050
type: SequenceAnnotation
persistent identity: annotation2531892
display id: annotation2531892
version: 1
top level: BBa_S05382
location: https://synbiohub.org/public/igem/BBa_S05382/annotation2531892/range2531892/1
component: BBa_K902050

https://synbiohub.org/public/igem/BBa_S05382/annotation2531892/range2531892/1
type: Range
persistent identity: range2531892
display id: range2531892
version: 1
top level: BBa_S05382
direction: direction/reverse
start: 2631
end: 3992
orientation: inline

dszA
type: ComponentDefinition
persistent identity: BBa K902050
display id: BBa_K902050
version: 1
was generated by: iGEM to SBOL conversion
description: DBT-sulfone monooxygenase (DszA) from R. erythropolis
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer.
owned by: james
owned by: myers
mutable description: A monooxygenase enzyme involved in the 4S desulfurization pathway. This protein encoded by this sequence carries out the third step in the pathway, converting DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 4 internal PstI cut-sites had to be mutated with site-directed mutagenesis.
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: DszA
sequence annotation: start
sequence annotation: G->A
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902050_sequence/1

DszA
type: SequenceAnnotation
persistent identity: annotation2205093
display id: annotation2205093
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205093/range2205093/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K902050/annotation2205093/range2205093/1
type: Range
persistent identity: range2205093
display id: range2205093
version: 1
top level: dszA
direction: direction/forward
start: 1
end: 1362
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2205051
display id: annotation2205051
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205051/range2205051/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K902050/annotation2205051/range2205051/1
type: Range
persistent identity: range2205051
display id: range2205051
version: 1
top level: dszA
direction: direction/forward
start: 1
end: 3
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205052
display id: annotation2205052
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205052/range2205052/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205052/range2205052/1
type: Range
persistent identity: range2205052
display id: range2205052
version: 1
top level: dszA
direction: direction/forward
start: 639
end: 639
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205053
display id: annotation2205053
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205053/range2205053/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205053/range2205053/1
type: Range
persistent identity: range2205053
display id: range2205053
version: 1
top level: dszA
direction: direction/forward
start: 678
end: 678
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205054
display id: annotation2205054
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205054/range2205054/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205054/range2205054/1
type: Range
persistent identity: range2205054
display id: range2205054
version: 1
top level: dszA
direction: direction/forward
start: 1011
end: 1011
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205055
display id: annotation2205055
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2205055/range2205055/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902050/annotation2205055/range2205055/1
type: Range
persistent identity: range2205055
display id: range2205055
version: 1
top level: dszA
direction: direction/forward
start: 1266
end: 1266
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211180
display id: annotation2211180
version: 1
top level: dszA
location: https://synbiohub.org/public/igem/BBa_K902050/annotation2211180/range2211180/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902050/annotation2211180/range2211180/1
type: Range
persistent identity: range2211180
display id: range2211180
version: 1
top level: dszA
direction: direction/forward
start: 1360
end: 1362
orientation: inline

BBa_S05381
type: ComponentDefinition
persistent identity: BBa S05381
display id: BBa_S05381
version: 1
was generated by: iGEM to SBOL conversion
description: S05380:B0034
created: 2016-12-09T12:00:00Z
modified: 2016-12-09T12:10:05Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_S05380
sequence annotation: BBa_S05380
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S05381_sequence/1

BBa_B0034
type: Component
persistent identity: component2531860
display id: component2531860
version: 1
top level: BBa_S05381
definition: BBa_B0034
access: public

BBa_S05380
type: Component
persistent identity: component2531858
display id: component2531858
version: 1
top level: BBa_S05381
definition: BBa_S05380
access: public

BBa_S05380
type: SequenceAnnotation
persistent identity: annotation2531858
display id: annotation2531858
version: 1
top level: BBa_S05381
location: https://synbiohub.org/public/igem/BBa_S05381/annotation2531858/range2531858/1
component: BBa_S05380

https://synbiohub.org/public/igem/BBa_S05381/annotation2531858/range2531858/1
type: Range
persistent identity: range2531858
display id: range2531858
version: 1
top level: BBa_S05381
direction: direction/reverse
start: 1
end: 2604
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2531860
display id: annotation2531860
version: 1
top level: BBa_S05381
location: https://synbiohub.org/public/igem/BBa_S05381/annotation2531860/range2531860/1
component: BBa_B0034

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type: Range
persistent identity: range2531860
display id: range2531860
version: 1
top level: BBa_S05381
direction: direction/reverse
start: 2613
end: 2624
orientation: inline

BBa_S05380
type: ComponentDefinition
persistent identity: BBa S05380
display id: BBa_S05380
version: 1
was generated by: iGEM to SBOL conversion
description: S05379:K804005
created: 2016-12-09T12:00:00Z
modified: 2016-12-09T12:09:17Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K804005
component: BBa_S05379
sequence annotation: BBa_S05379
sequence annotation: BBa_K804005
sequence: https://synbiohub.org/public/igem/BBa_S05380_sequence/1

BBa_K804005
type: Component
persistent identity: component2531837
display id: component2531837
version: 1
top level: BBa_S05380
definition: BBa_K804005
access: public

BBa_S05379
type: Component
persistent identity: component2531836
display id: component2531836
version: 1
top level: BBa_S05380
definition: BBa_S05379
access: public

BBa_S05379
type: SequenceAnnotation
persistent identity: annotation2531836
display id: annotation2531836
version: 1
top level: BBa_S05380
location: https://synbiohub.org/public/igem/BBa_S05380/annotation2531836/range2531836/1
component: BBa_S05379

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type: Range
persistent identity: range2531836
display id: range2531836
version: 1
top level: BBa_S05380
direction: direction/reverse
start: 1
end: 1344
orientation: inline

BBa_K804005
type: SequenceAnnotation
persistent identity: annotation2531837
display id: annotation2531837
version: 1
top level: BBa_S05380
location: https://synbiohub.org/public/igem/BBa_S05380/annotation2531837/range2531837/1
component: BBa_K804005

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type: Range
persistent identity: range2531837
display id: range2531837
version: 1
top level: BBa_S05380
direction: direction/reverse
start: 1351
end: 2604
orientation: inline

BBa_K804005
type: ComponentDefinition
persistent identity: BBa K804005
display id: BBa_K804005
version: 1
was generated by: iGEM to SBOL conversion
description: DszC coding gene (from DszABC operon)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/L37363.1 (GenBank: L37363.1)
owned by: james
owned by: myers
mutable description: This is a DszC coding gene from the DszABC operon in the 4S pathway of Rhodococcus erythropolis IGTS8. It encodes one of the three biodesulfurizing enzymes in the DszABC operon. DszC enzymes have been shown to catalyze dibenzothiophene (DBT)to dibenzothiophene-5-oxide (DBTO) in the first reaction and then from DBTO to DBT sulfoxide (DBTO2) in the second reaction, both in the presence of NADH, FMN and flavin reductases.
discontinued: false
dominant: false
group u list: _1060_
m user id: 0
owner id: 9774
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 2 PstI sites were removed using site-directed mutagenesis
star: false
creator: Joe Ho
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K804005_sequence/1

BBa_S05379
type: ComponentDefinition
persistent identity: BBa S05379
display id: BBa_S05379
version: 1
was generated by: iGEM to SBOL conversion
description: K902054:B0034
created: 2016-12-09T12:00:00Z
modified: 2016-12-09T12:08:19Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 24251
owner id: 24251
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Gilles Yvan Buck
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_K902054
sequence annotation: BBa_K902054
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S05379_sequence/1

BBa_B0034
type: Component
persistent identity: component2531817
display id: component2531817
version: 1
top level: BBa_S05379
definition: BBa_B0034
access: public

BBa_K902054
type: Component
persistent identity: component2531815
display id: component2531815
version: 1
top level: BBa_S05379
definition: BBa_K902054
access: public

BBa_K902054
type: SequenceAnnotation
persistent identity: annotation2531815
display id: annotation2531815
version: 1
top level: BBa_S05379
location: https://synbiohub.org/public/igem/BBa_S05379/annotation2531815/range2531815/1
component: BBa_K902054

https://synbiohub.org/public/igem/BBa_S05379/annotation2531815/range2531815/1
type: Range
persistent identity: range2531815
display id: range2531815
version: 1
top level: BBa_S05379
direction: direction/reverse
start: 1
end: 1324
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2531817
display id: annotation2531817
version: 1
top level: BBa_S05379
location: https://synbiohub.org/public/igem/BBa_S05379/annotation2531817/range2531817/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05379/annotation2531817/range2531817/1
type: Range
persistent identity: range2531817
display id: range2531817
version: 1
top level: BBa_S05379
direction: direction/reverse
start: 1333
end: 1344
orientation: inline

BBa_K902054
type: ComponentDefinition
persistent identity: BBa K902054
display id: BBa_K902054
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible DszB generator (J04500-DszB)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: DszB is from a plasmid from an environmental isolate of Rhodococcus shown to be an active desulfurizer, promotor from registry.
owned by: james
owned by: myers
mutable description: Inducible expression (using LacI promotor J04500) of DszB
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: See DszB mutations
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K902052
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K902052
sequence: https://synbiohub.org/public/igem/BBa_K902054_sequence/1

BBa_K902052
type: Component
persistent identity: component2205166
display id: component2205166
version: 1
top level: BBa_K902054
definition: dszB
access: public

BBa_J04500
type: Component
persistent identity: component2205160
display id: component2205160
version: 1
top level: BBa_K902054
definition: BBa_J04500
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2205160
display id: annotation2205160
version: 1
top level: BBa_K902054
location: https://synbiohub.org/public/igem/BBa_K902054/annotation2205160/range2205160/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K902054/annotation2205160/range2205160/1
type: Range
persistent identity: range2205160
display id: range2205160
version: 1
top level: BBa_K902054
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K902052
type: SequenceAnnotation
persistent identity: annotation2205166
display id: annotation2205166
version: 1
top level: BBa_K902054
location: https://synbiohub.org/public/igem/BBa_K902054/annotation2205166/range2205166/1
component: BBa_K902052

https://synbiohub.org/public/igem/BBa_K902054/annotation2205166/range2205166/1
type: Range
persistent identity: range2205166
display id: range2205166
version: 1
top level: BBa_K902054
direction: direction/reverse
start: 227
end: 1324
orientation: inline

dszB
type: ComponentDefinition
persistent identity: BBa K902052
display id: BBa_K902052
version: 1
was generated by: iGEM to SBOL conversion
description: HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:43Z
mutable provenance: Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.
owned by: james
owned by: myers
mutable description: DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.
discontinued: false
dominant: false
experience: experience/None
group u list: _1167_
m user id: 0
owner id: 13399
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.
star: false
creator: Lisa Oberding, Ali Honarmand
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: DszB
sequence annotation: start
sequence annotation: Y63F
sequence annotation: G->A
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K902052_sequence/1

DszB
type: SequenceAnnotation
persistent identity: annotation2205132
display id: annotation2205132
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205132/range2205132/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K902052/annotation2205132/range2205132/1
type: Range
persistent identity: range2205132
display id: range2205132
version: 1
top level: dszB
direction: direction/forward
start: 1
end: 1098
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2205133
display id: annotation2205133
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205133/range2205133/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K902052/annotation2205133/range2205133/1
type: Range
persistent identity: range2205133
display id: range2205133
version: 1
top level: dszB
direction: direction/forward
start: 1
end: 3
orientation: inline

Y63F
type: SequenceAnnotation
persistent identity: annotation2205134
display id: annotation2205134
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205134/range2205134/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205134/range2205134/1
type: Range
persistent identity: range2205134
display id: range2205134
version: 1
top level: dszB
direction: direction/forward
start: 187
end: 189
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205135
display id: annotation2205135
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205135/range2205135/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K902052/annotation2205135/range2205135/1
type: Range
persistent identity: range2205135
display id: range2205135
version: 1
top level: dszB
direction: direction/forward
start: 339
end: 339
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation2205136
display id: annotation2205136
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2205136/range2205136/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K902052/annotation2205136/range2205136/1
type: Range
persistent identity: range2205136
display id: range2205136
version: 1
top level: dszB
direction: direction/forward
start: 1026
end: 1026
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2211179
display id: annotation2211179
version: 1
top level: dszB
location: https://synbiohub.org/public/igem/BBa_K902052/annotation2211179/range2211179/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K902052/annotation2211179/range2211179/1
type: Range
persistent identity: range2211179
display id: range2211179
version: 1
top level: dszB
direction: direction/forward
start: 1096
end: 1098
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

https://synbiohub.org/public/igem/BBa_S05389_sequence/1
type: Sequence
persistent identity: BBa S05389 sequence
display id: BBa_S05389_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902057_sequence/1
type: Sequence
persistent identity: BBa K902057 sequence
display id: BBa_K902057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaattagatgaacaacgcctgcgctttcgtgacgcaatggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggcaacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05388_sequence/1
type: Sequence
persistent identity: BBa S05388 sequence
display id: BBa_S05388_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05382_sequence/1
type: Sequence
persistent identity: BBa S05382 sequence
display id: BBa_S05382_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902050_sequence/1
type: Sequence
persistent identity: BBa K902050 sequence
display id: BBa_K902050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgactcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgtactctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctggacaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgaccgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctcgatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcattaatcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctgggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccacggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcctgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgcaggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtgatgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgggactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcgggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcggaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagctgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagttcgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccacttgggtctgcgcgtaccacaactgcaaggacaaccttcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05381_sequence/1
type: Sequence
persistent identity: BBa S05381 sequence
display id: BBa_S05381_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05380_sequence/1
type: Sequence
persistent identity: BBa S05380 sequence
display id: BBa_S05380_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaatactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K804005_sequence/1
type: Sequence
persistent identity: BBa K804005 sequence
display id: BBa_K804005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcccgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaagacctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccatcgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatgatcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatgcctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaatggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccgcagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcggcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaacgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtctatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccggtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgccgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaactgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtgatcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgacccggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05379_sequence/1
type: Sequence
persistent identity: BBa S05379 sequence
display id: BBa_S05379_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902054_sequence/1
type: Sequence
persistent identity: BBa K902054 sequence
display id: BBa_K902054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K902052_sequence/1
type: Sequence
persistent identity: BBa K902052 sequence
display id: BBa_K902052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacaagccgcgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtacccaacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagggcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggttgcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagcccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagctgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgcacacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgctgagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttggccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggtggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggccttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgcacgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacgcctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtcgccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z