LOCUS BBa_I12027 2067 bp DNA linear 22-Nov-2024 DEFINITION 434 cI Generator (pBAD/araC promoter) ACCESSION BBa_I12027 VERSION BBa_I12027.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1905 /label=cI 434 misc_feature 1867..1905 /label=LVA misc_feature 1237..1905 /label=BBa_C0052 misc_feature 1906..1930 /label=Help:Barcodes CDS 1237..1905 /label=BBa_C0052 stem_loop 1950..1993 /label=stem_loop misc_feature 1939..2018 /label=BBa_B0010 terminator 1939..2018 /label=BBa_B0010 stem_loop 2034..2053 /label=T7 TE misc_feature 2060..2060 /label=stop polyA_site 2054..2067 /label=polya misc_feature 2027..2067 /label=BBa_B0012 terminator 2027..2067 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gtatttcttc cagggtaaaa 1261 agcaaaagaa tccagcttgg acttaaccag gctgaacttg ctcaaaaggt ggggactacc 1321 cagcagtcta tagagcagct cgaaaacggt aaaactaagc gaccacgctt tttaccagaa 1381 cttgcgtcag ctcttggcgt aagtgttgac tggctgctca atggcacctc tgattcgaat 1441 gttagatttg ttgggcacgt tgagcccaaa gggaaatatc cattgattag catggttaga 1501 gctggttcgt ggtgtgaagc ttgtgaaccc tacgatatca aggacattga tgaatggtat 1561 gacagtgacg ttaacttatt aggcaatgga ttctggctga aggttgaagg tgattccatg 1621 acctcacctg taggtcaaag catccctgaa ggtcatatgg tgttagtaga tactggacgg 1681 gagccagtga atggaagcct tgttgtagcc aaactgactg acgcgaacga agcaacattc 1741 aagaaactgg tcatagatgg cggtcagaag tacctgaaag gcctgaatcc ttcatggcct 1801 atgactccta tcaacggaaa ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa 1861 ttcgtagctg caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag 1921 tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1981 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2041 cttcgggtgg gcctttctgc gtttata // LOCUS BBa_S50452 2067 bp DNA linear 22-Nov-2024 DEFINITION I0500.P0452 ACCESSION BBa_S50452 VERSION BBa_S50452.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1905 /label=cI 434 misc_feature 1867..1905 /label=LVA misc_feature 1237..1905 /label=BBa_C0052 misc_feature 1906..1930 /label=Help:Barcodes CDS 1237..1905 /label=BBa_C0052 stem_loop 1950..1993 /label=stem_loop misc_feature 1939..2018 /label=BBa_B0010 terminator 1939..2018 /label=BBa_B0010 stem_loop 2034..2053 /label=T7 TE misc_feature 2060..2060 /label=stop polyA_site 2054..2067 /label=polya misc_feature 2027..2067 /label=BBa_B0012 terminator 2027..2067 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gtatttcttc cagggtaaaa 1261 agcaaaagaa tccagcttgg acttaaccag gctgaacttg ctcaaaaggt ggggactacc 1321 cagcagtcta tagagcagct cgaaaacggt aaaactaagc gaccacgctt tttaccagaa 1381 cttgcgtcag ctcttggcgt aagtgttgac tggctgctca atggcacctc tgattcgaat 1441 gttagatttg ttgggcacgt tgagcccaaa gggaaatatc cattgattag catggttaga 1501 gctggttcgt ggtgtgaagc ttgtgaaccc tacgatatca aggacattga tgaatggtat 1561 gacagtgacg ttaacttatt aggcaatgga ttctggctga aggttgaagg tgattccatg 1621 acctcacctg taggtcaaag catccctgaa ggtcatatgg tgttagtaga tactggacgg 1681 gagccagtga atggaagcct tgttgtagcc aaactgactg acgcgaacga agcaacattc 1741 aagaaactgg tcatagatgg cggtcagaag tacctgaaag gcctgaatcc ttcatggcct 1801 atgactccta tcaacggaaa ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa 1861 ttcgtagctg caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag 1921 tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1981 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2041 cttcgggtgg gcctttctgc gtttata //