LOCUS BBa_T1004 2284 bp DNA linear 22-Nov-2024 DEFINITION Test part ACCESSION BBa_T1004 VERSION BBa_T1004.1 FEATURES Location/Qualifiers misc_feature 21..26 /label=[10] CDS 1..52 /label=BBa_T1001 stem_loop 901..944 /label=stem_loop misc_feature 890..969 /label=BBa_B0010 terminator 890..969 /label=BBa_B0010 stem_loop 985..1004 /label=T7 TE misc_feature 1011..1011 /label=stop polyA_site 1005..1018 /label=polya misc_feature 978..1018 /label=BBa_B0012 terminator 978..1018 /label=BBa_B0012 terminator 890..1018 /label=BBa_B0015 misc_feature 81..86 /label=[10] CDS 61..112 /label=BBa_T1001 misc_feature 121..190 /label=Spacer-1 misc_feature 121..190 /label=BBa_B0040 misc_feature 121..190 /label=BBa_B0040 CDS 200..816 /label=tetR misc_feature 817..850 /label=SsrA misc_feature 857..881 /label=Help:Barcodes CDS 197..881 /label=BBa_C0040 stem_loop 897..916 /label=T7 TE misc_feature 923..923 /label=stop polyA_site 917..930 /label=polya misc_feature 890..930 /label=BBa_B0012 terminator 890..930 /label=BBa_B0012 misc_feature 61..1018 /label=BBa_T1002 misc_feature 1047..1052 /label=[10] CDS 1027..1078 /label=BBa_T1001 misc_feature 1087..1105 /label=TetR 1 misc_feature 1087..1140 /label=BBa_R0040 promoter 1106..1111 /label=-35 misc_feature 1112..1130 /label=TetR 2 promoter 1129..1134 /label=-10 promoter 1087..1140 /label=BBa_R0040 misc_feature 1153..1156 /label=conserved RBS 1149..1160 /label=BBa_B0034 misc_feature 1167..1168 /label=prefix misc_feature 1340..1340 /label=T misc_feature 1658..1658 /label=A CDS 1167..1916 /label=luxR misc_feature 1167..1922 /label=BBa_C0062 misc_feature 1923..1947 /label=Help:Barcodes CDS 1167..1922 /label=BBa_C0062 stem_loop 1967..2010 /label=stem_loop misc_feature 1956..2035 /label=BBa_B0010 terminator 1956..2035 /label=BBa_B0010 stem_loop 2051..2070 /label=T7 TE misc_feature 2077..2077 /label=stop polyA_site 2071..2084 /label=polya misc_feature 2044..2084 /label=BBa_B0012 terminator 2044..2084 /label=BBa_B0012 terminator 1956..2084 /label=BBa_B0015 misc_feature 2093..2112 /label=LuxR/HSL promoter 2112..2117 /label=-35 promoter 2134..2139 /label=-10 misc_feature 2145..2145 /label=start misc_feature 2093..2147 /label=BBa_R0062 promoter 2093..2147 /label=BBa_R0062 misc_feature 1087..2147 /label=BBa_F2620 stem_loop 2167..2210 /label=stem_loop misc_feature 2156..2235 /label=BBa_B0010 terminator 2156..2235 /label=BBa_B0010 stem_loop 2251..2270 /label=T7 TE misc_feature 2277..2277 /label=stop polyA_site 2271..2284 /label=polya misc_feature 2244..2284 /label=BBa_B0012 terminator 2244..2284 /label=BBa_B0012 terminator 2156..2284 /label=BBa_B0015 ORIGIN 1 taagaaaaaa aaatctagaa aaaaaaatct agaaaaaaaa aaaagaattt tttactagag 61 taagaaaaaa aaatctagaa aaaaaaatct agaaaaaaaa aaaagaattt tttactagag 121 aggttctgtt aagtaactga acccaatgtc gttagtgacg cttacctctt aagaggtcac 181 tgacctaaca tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga 241 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg 301 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt 361 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg 421 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga 481 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa 541 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag 601 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa 661 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga 721 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 781 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc 841 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 901 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 961 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 1021 ctagagtaag aaaaaaaaat ctagaaaaaa aaatctagaa aaaaaaaaaa gaatttttta 1081 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac 1141 tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac acatacagaa 1201 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata 1261 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg 1321 ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg 1381 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa 1441 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag 1501 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg 1561 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt 1621 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa 1681 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag 1741 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc 1801 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc 1861 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat 1921 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 1981 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2041 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 2101 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagccagg 2161 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2221 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2281 tata //