LOCUS       BBa_T1015               1032 bp    DNA     linear       05-Nov-2024
DEFINITION  Kill me
ACCESSION   BBa_T1015
VERSION     BBa_T1015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..45
                     /label=Stop using
     misc_feature    1..66
                     /label=BBa_T1008
     stem_loop       915..958
                     /label=stem_loop
     misc_feature    904..983
                     /label=BBa_B0010
     terminator      904..983
                     /label=BBa_B0010
     stem_loop       999..1018
                     /label=T7 TE
     misc_feature    1025..1025
                     /label=stop
     polyA_site      1019..1032
                     /label=polya
     misc_feature    992..1032
                     /label=BBa_B0012
     terminator      992..1032
                     /label=BBa_B0012
     misc_feature    95..100
                     /label=[10]
     CDS             75..126
                     /label=BBa_T1001
     misc_feature    135..204
                     /label=Spacer-1
     misc_feature    135..204
                     /label=BBa_B0040
     misc_feature    135..204
                     /label=BBa_B0040
     CDS             214..830
                     /label=tetR
     misc_feature    831..864
                     /label=SsrA
     misc_feature    871..895
                     /label=Help:Barcodes
     CDS             211..895
                     /label=BBa_C0040
     stem_loop       911..930
                     /label=T7 TE
     misc_feature    937..937
                     /label=stop
     polyA_site      931..944
                     /label=polya
     misc_feature    904..944
                     /label=BBa_B0012
     terminator      904..944
                     /label=BBa_B0012
     misc_feature    75..1032
                     /label=BBa_T1002
ORIGIN
        1 actcttagcg tatcgtcgtc tgtctgctag ctagtcgtag gcgtagctag ctacgtgggc
       61 ggagcttact agagtaagaa aaaaaaatct agaaaaaaaa atctagaaaa aaaaaaaaga
      121 attttttact agagaggttc tgttaagtaa ctgaacccaa tgtcgttagt gacgcttacc
      181 tcttaagagg tcactgacct aacatactag atgtccagat tagataaaag taaagtgatt
      241 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
      301 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
      361 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
      421 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
      481 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
      541 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
      601 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
      661 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
      721 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
      781 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac
      841 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta
      901 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      961 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1021 tctgcgttta ta
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