BBa_T1015
type: ComponentDefinition
persistent identity: BBa T1015
display id: BBa_T1015
version: 1
was generated by: iGEM to SBOL conversion
description: Kill me
created: 2011-05-26T11:00:00Z
modified: 2015-05-08T01:14:52Z
mutable provenance: none
owned by: myers
owned by: james
mutable description: just testing
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Randy Rettberg
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_T1002
component: BBa_B0012
component: BBa_B0010
component: BBa_T1008
sequence annotation: BBa_T1008
sequence annotation: BBa_T1002
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_T1015_sequence/1

BBa_T1002
type: Component
persistent identity: component2373689
display id: component2373689
version: 1
top level: BBa_T1015
definition: BBa_T1002
access: public

BBa_B0012
type: Component
persistent identity: component2373685
display id: component2373685
version: 1
top level: BBa_T1015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2373682
display id: component2373682
version: 1
top level: BBa_T1015
definition: BBa_B0010
access: public

BBa_T1008
type: Component
persistent identity: component2373674
display id: component2373674
version: 1
top level: BBa_T1015
definition: BBa_T1008
access: public

BBa_T1008
type: SequenceAnnotation
persistent identity: annotation2373674
display id: annotation2373674
version: 1
top level: BBa_T1015
location: https://synbiohub.org/public/igem/BBa_T1015/annotation2373674/range2373674/1
component: BBa_T1008

https://synbiohub.org/public/igem/BBa_T1015/annotation2373674/range2373674/1
type: Range
persistent identity: range2373674
display id: range2373674
version: 1
top level: BBa_T1015
direction: direction/reverse
start: 1
end: 66
orientation: inline

BBa_T1002
type: SequenceAnnotation
persistent identity: annotation2373689
display id: annotation2373689
version: 1
top level: BBa_T1015
location: https://synbiohub.org/public/igem/BBa_T1015/annotation2373689/range2373689/1
component: BBa_T1002

https://synbiohub.org/public/igem/BBa_T1015/annotation2373689/range2373689/1
type: Range
persistent identity: range2373689
display id: range2373689
version: 1
top level: BBa_T1015
direction: direction/reverse
start: 75
end: 1032
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2373682
display id: annotation2373682
version: 1
top level: BBa_T1015
location: https://synbiohub.org/public/igem/BBa_T1015/annotation2373682/range2373682/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_T1015/annotation2373682/range2373682/1
type: Range
persistent identity: range2373682
display id: range2373682
version: 1
top level: BBa_T1015
direction: direction/forward
start: 904
end: 983
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2373685
display id: annotation2373685
version: 1
top level: BBa_T1015
location: https://synbiohub.org/public/igem/BBa_T1015/annotation2373685/range2373685/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_T1015/annotation2373685/range2373685/1
type: Range
persistent identity: range2373685
display id: range2373685
version: 1
top level: BBa_T1015
direction: direction/forward
start: 992
end: 1032
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_T1008
type: ComponentDefinition
persistent identity: BBa T1008
display id: BBa_T1008
version: 1
was generated by: iGEM to SBOL conversion
description: Example part
created: 2011-05-26T11:00:00Z
modified: 2015-05-08T01:14:52Z
mutable provenance: Randy s brain
owned by: james
owned by: myers
mutable description: This is a part used as an example in the 2011 workshop
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 25
owning group id: 397
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Don't use this part
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: Stop using
sequence: https://synbiohub.org/public/igem/BBa_T1008_sequence/1

Stop using
type: SequenceAnnotation
persistent identity: annotation2120506
display id: annotation2120506
version: 1
top level: BBa_T1008
location: https://synbiohub.org/public/igem/BBa_T1008/annotation2120506/range2120506/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_T1008/annotation2120506/range2120506/1
type: Range
persistent identity: range2120506
display id: range2120506
version: 1
top level: BBa_T1008
direction: direction/forward
start: 1
end: 45
orientation: inline

BBa_T1002
type: ComponentDefinition
persistent identity: BBa T1002
display id: BBa_T1002
version: 1
was generated by: iGEM to SBOL conversion
description: Testing the categories
created: 2009-05-09T11:00:00Z
modified: 2015-05-08T01:14:52Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: test
discontinued: true
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 46
sample status: Discontinued
status: status/Deleted
bookmark: true
mutable notes: test
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000110
role: partType/DNA
component: BBa_T1001
component: BBa_C0040
component: BBa_B0040
component: BBa_B0012
sequence annotation: BBa_T1001
sequence annotation: BBa_B0040
sequence annotation: BBa_C0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_T1002_sequence/1

BBa_T1001
type: Component
persistent identity: component2383824
display id: component2383824
version: 1
top level: BBa_T1002
definition: BBa_T1001
access: public

BBa_C0040
type: Component
persistent identity: component2383830
display id: component2383830
version: 1
top level: BBa_T1002
definition: tetR
access: public

BBa_B0040
type: Component
persistent identity: component2383826
display id: component2383826
version: 1
top level: BBa_T1002
definition: spacer
access: public

BBa_B0012
type: Component
persistent identity: component2383831
display id: component2383831
version: 1
top level: BBa_T1002
definition: BBa_B0012
access: public

BBa_T1001
type: SequenceAnnotation
persistent identity: annotation2383824
display id: annotation2383824
version: 1
top level: BBa_T1002
location: https://synbiohub.org/public/igem/BBa_T1002/annotation2383824/range2383824/1
component: BBa_T1001

https://synbiohub.org/public/igem/BBa_T1002/annotation2383824/range2383824/1
type: Range
persistent identity: range2383824
display id: range2383824
version: 1
top level: BBa_T1002
direction: direction/reverse
start: 1
end: 52
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation2383826
display id: annotation2383826
version: 1
top level: BBa_T1002
location: https://synbiohub.org/public/igem/BBa_T1002/annotation2383826/range2383826/1
component: BBa_B0040

https://synbiohub.org/public/igem/BBa_T1002/annotation2383826/range2383826/1
type: Range
persistent identity: range2383826
display id: range2383826
version: 1
top level: BBa_T1002
direction: direction/forward
start: 61
end: 130
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2383830
display id: annotation2383830
version: 1
top level: BBa_T1002
location: https://synbiohub.org/public/igem/BBa_T1002/annotation2383830/range2383830/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_T1002/annotation2383830/range2383830/1
type: Range
persistent identity: range2383830
display id: range2383830
version: 1
top level: BBa_T1002
direction: direction/reverse
start: 137
end: 821
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2383831
display id: annotation2383831
version: 1
top level: BBa_T1002
location: https://synbiohub.org/public/igem/BBa_T1002/annotation2383831/range2383831/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_T1002/annotation2383831/range2383831/1
type: Range
persistent identity: range2383831
display id: range2383831
version: 1
top level: BBa_T1002
direction: direction/forward
start: 830
end: 870
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_T1001
type: ComponentDefinition
persistent identity: BBa T1001
display id: BBa_T1001
version: 1
was generated by: iGEM to SBOL conversion
description: Kill this part
created: 2009-05-04T11:00:00Z
modified: 2015-05-08T01:14:52Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Test part for the assembly system mechanism
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 46
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Randy Rettberg
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: [10]
sequence: https://synbiohub.org/public/igem/BBa_T1001_sequence/1

[10]
type: SequenceAnnotation
persistent identity: annotation2378354
display id: annotation2378354
version: 1
top level: BBa_T1001
location: https://synbiohub.org/public/igem/BBa_T1001/annotation2378354/range2378354/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_T1001/annotation2378354/range2378354/1
type: Range
persistent identity: range2378354
display id: range2378354
version: 1
top level: BBa_T1001
direction: direction/forward
start: 21
end: 26
orientation: inline

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer.1 (generic)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
star: true
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

https://synbiohub.org/public/igem/BBa_T1015_sequence/1
type: Sequence
persistent identity: BBa T1015 sequence
display id: BBa_T1015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcttagcgtatcgtcgtctgtctgctagctagtcgtaggcgtagctagctacgtgggcggagcttactagagtaagaaaaaaaaatctagaaaaaaaaatctagaaaaaaaaaaaagaattttttactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_T1008_sequence/1
type: Sequence
persistent identity: BBa T1008 sequence
display id: BBa_T1008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcttagcgtatcgtcgtctgtctgctagctagtcgtaggcgtagctagctacgtgggcggagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_T1002_sequence/1
type: Sequence
persistent identity: BBa T1002 sequence
display id: BBa_T1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagaaaaaaaaatctagaaaaaaaaatctagaaaaaaaaaaaagaattttttactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_T1001_sequence/1
type: Sequence
persistent identity: BBa T1001 sequence
display id: BBa_T1001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagaaaaaaaaatctagaaaaaaaaatctagaaaaaaaaaaaagaattttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0040_sequence/1
type: Sequence
persistent identity: BBa B0040 sequence
display id: BBa_B0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z