BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0051 1 cI lam promoter (lambda cI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false true _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2025 1 OR2 range2025 1 1 17 annotation2022 1 -10 range2022 1 38 43 annotation7067 1 BBa_R0051 range7067 1 1 49 annotation2023 1 -35 range2023 1 15 20 annotation2024 1 OR1 range2024 1 25 41 BBa_T4002 1 BBa_T4002 luxC device regulated by lambda cl 2012-02-22T12:00:00Z 2015-05-08T01:14:52Z Used a strong RBS to produce high levels of expression of the LuxC protein. Uses a negatively regulated promoter (BBa_R0051), strong RBS (BBa_B0034), luxC coding region (BBa_T4001), and a double terminator (BBa_B0015). Will generate luxC in absence of lambda cl. false false _1_ 0 4206 1 Not in stock false This is a composite part including the luxC protein coding region encoded in part BBa_T4000. false Vinoo Selvarajah component2170398 1 BBa_B0015 component2170391 1 BBa_T4000 component2170382 1 BBa_R0051 component2170387 1 BBa_B0034 annotation2170382 1 BBa_R0051 range2170382 1 1 49 annotation2170391 1 BBa_T4000 range2170391 1 76 1515 annotation2170387 1 BBa_B0034 range2170387 1 58 69 annotation2170398 1 BBa_B0015 range2170398 1 1524 1652 BBa_T4000 1 BBa_T4000 luxC 2012-02-22T12:00:00Z 2015-05-08T01:14:52Z Vibrio fischeri Test description. false false _1_ 0 4206 1 Not in stock false Removal of illegal restriction sites for the BioBrick Standard false Vinoo Selvarajah annotation2170337 1 ATG Start Codon range2170337 1 1 3 annotation2170338 1 luxC coding region range2170338 1 1 1440 annotation2170339 1 TAA Stop Codon range2170339 1 1438 1440 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0051_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_T4002_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgattaaatgtattccgatgataattaaaggcgtagttcaagattttgataataacgcttgtaaagaaataaatttagatagtggtaataaaattaaattatctcttcttactgaagattcagttttaagatcattaaattctaaagaaaaagttgatcttaacttaaatcagattgtaaatttcctatataccgtaggtcaacgatggaaaaatgaagaatataatcgaagacgaacttatattcgtgagttaaaaaaatatcttggatattctgatgaaatggctagattagaagccaattggatagctatgttattgtgttctaaaagtgctttgtatgacattgttaattacgatttgggatctatacacgtgttggatgaatggcttccgcgtggtgattgctatgttaaagcacaagcaaaaggtgtttctattcacttgttggctggtaatgttccactatcaggggtgacatctattttgcgtgctattttgacaaaaaatgaatgcataattaaaacctcttcttcagatccgtttactgcaacggctttggcttcgagttttattgatgttaatgcagagcatccaataactaaatcaatgtctgttatgtattggccgcataatgaagatatgactctacctcaaagaattatgaatcatgcagacattgttattgcttggggaggagaagaggcgattaaatgggcggcaaaacactcaccaccgcatgccgatgttcttaaatttggaccaaagaaaagtttaagcattattgaggagcctgaagacatggaggaagctgcaatgggggttgcccatgatatctgtttctatgatcagcaagcctgcttctcaactcaagacgtttattatatcggagaacatttgcctttatttttaagtgaacttgaaaaacagctagatcgatacgcaaagattttaccaaaaggtttaaaaaattttgatgaaaaagcggcgttttctcttaccgaaagagagggaatatttgctggatatgacgtaaaaaagggggataaccaagcttggttaatgattatatctcctacaaatagctctggaaatcagccattatctcgaagcgtgtatatacatcaagtctctgatattaatgaggtgcttccttttgtaaataaaaatagtacacaaaccgtctctatttacccttgggaggcttcactgaaatatagagacaaattagcgatgagtggtgctgagagaatcgttgaatcaggaatgaataatattttcagagttggaggagcacatgattcattatctcctctccagtatttagttaggtttacctcccatgaaagaccatttcattatacaaccaaggatgttgcggttgaaattgaacaaacgcgttacttagaagaagacaaatttttggtttttgtcccataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_T4000_sequence 1 atgattaaatgtattccgatgataattaaaggcgtagttcaagattttgataataacgcttgtaaagaaataaatttagatagtggtaataaaattaaattatctcttcttactgaagattcagttttaagatcattaaattctaaagaaaaagttgatcttaacttaaatcagattgtaaatttcctatataccgtaggtcaacgatggaaaaatgaagaatataatcgaagacgaacttatattcgtgagttaaaaaaatatcttggatattctgatgaaatggctagattagaagccaattggatagctatgttattgtgttctaaaagtgctttgtatgacattgttaattacgatttgggatctatacacgtgttggatgaatggcttccgcgtggtgattgctatgttaaagcacaagcaaaaggtgtttctattcacttgttggctggtaatgttccactatcaggggtgacatctattttgcgtgctattttgacaaaaaatgaatgcataattaaaacctcttcttcagatccgtttactgcaacggctttggcttcgagttttattgatgttaatgcagagcatccaataactaaatcaatgtctgttatgtattggccgcataatgaagatatgactctacctcaaagaattatgaatcatgcagacattgttattgcttggggaggagaagaggcgattaaatgggcggcaaaacactcaccaccgcatgccgatgttcttaaatttggaccaaagaaaagtttaagcattattgaggagcctgaagacatggaggaagctgcaatgggggttgcccatgatatctgtttctatgatcagcaagcctgcttctcaactcaagacgtttattatatcggagaacatttgcctttatttttaagtgaacttgaaaaacagctagatcgatacgcaaagattttaccaaaaggtttaaaaaattttgatgaaaaagcggcgttttctcttaccgaaagagagggaatatttgctggatatgacgtaaaaaagggggataaccaagcttggttaatgattatatctcctacaaatagctctggaaatcagccattatctcgaagcgtgtatatacatcaagtctctgatattaatgaggtgcttccttttgtaaataaaaatagtacacaaaccgtctctatttacccttgggaggcttcactgaaatatagagacaaattagcgatgagtggtgctgagagaatcgttgaatcaggaatgaataatattttcagagttggaggagcacatgattcattatctcctctccagtatttagttaggtttacctcccatgaaagaccatttcattatacaaccaaggatgttgcggttgaaattgaacaaacgcgttacttagaagaagacaaatttttggtttttgtcccataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z