LOCUS BBa_T5000 4456 bp DNA linear 22-Dec-2024 DEFINITION lacI inhibited lux receiver test ACCESSION BBa_T5000 VERSION BBa_T5000.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 2326..2364 /label=LVA CDS 1237..2364 /label=lacI-LVA misc_feature 1237..2364 /label=BBa_C0012 misc_feature 2365..2389 /label=Help:Barcodes CDS 1237..2364 /label=BBa_C0012 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 terminator 2398..2526 /label=BBa_B0015 promoter 2535..2569 /label=BBa_J23101 misc_feature 2582..2585 /label=conserved RBS 2578..2589 /label=BBa_B0034 CDS 2596..3351 /label=BBa_C0362 stem_loop 3371..3414 /label=stem_loop misc_feature 3360..3439 /label=BBa_B0010 terminator 3360..3439 /label=BBa_B0010 stem_loop 3455..3474 /label=T7 TE misc_feature 3481..3481 /label=stop polyA_site 3475..3488 /label=polya misc_feature 3448..3488 /label=BBa_B0012 terminator 3448..3488 /label=BBa_B0012 terminator 3360..3488 /label=BBa_B0015 promoter 3516..3570 /label=plac misc_feature 3516..3522 /label=-35 misc_feature 3538..3544 /label=-10 misc_feature 3523..3537 /label=O1 misc_feature 3545..3570 /label=O1 misc_feature 3497..3515 /label=luxR binding site promoter 3497..3570 /label=BBa_I751502 misc_feature 3583..3586 /label=conserved RBS 3579..3590 /label=BBa_B0034 CDS 3597..4319 /label=BBa_E0030 stem_loop 4339..4382 /label=stem_loop misc_feature 4328..4407 /label=BBa_B0010 terminator 4328..4407 /label=BBa_B0010 stem_loop 4423..4442 /label=T7 TE misc_feature 4449..4449 /label=stop polyA_site 4443..4456 /label=polya misc_feature 4416..4456 /label=BBa_B0012 terminator 4416..4456 /label=BBa_B0012 terminator 4328..4456 /label=BBa_B0015 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagtttaca gctagctcag tcctaggtat tatgctagct actagagaaa 2581 gaggagaaat actagatgaa aaacatcaac gcggacgaca cctaccgtat cattaacaaa 2641 atcaaagcgt gtcgttctaa caacgatatt aaccaatgcc tgtctgatat gaccaaaatg 2701 gttcactgtg aatattatct gctggccatc atttatccgc acagcatggt aaaatccgat 2761 atttctattc tggataacta cccaaaaaaa tggcgtcagt actatgatga cgccaacctg 2821 atcaagtacg atccgatcgt agattactct aactctaacc attccccgat taactggaat 2881 atctttgaga acaacgcggt taacaaaaag tccccaaacg ttatcaaaga agcaaaaacc 2941 agcggtctga tcaccggttt cagcttcccg attcataccg cgaacaacgg cttcggtatg 3001 ctgtcttttg cgcactccga aaaagacaac tacatcgatt ctctgttcct gcacgcgtgc 3061 atgaacattc ctctgatcgt tccgtccctg gttgataact atcgcaagat taacattgcc 3121 aataacaaaa gcaacaacga cctgaccaaa cgcgagaaag aatgcctggc atgggcgtgc 3181 gaaggtaagt cctcttggga catctccaaa attctgggtt gctctgaacg caccgtaact 3241 ttccatctga ccaacgcgca gatgaaactg aacaccacga atcgttgtca atctatcagc 3301 aaagctatcc tgaccggtgc gatcgattgc ccgtatttca aaaactaata atactagagc 3361 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3421 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3481 cgtttatata ctagagacct gtaggatcgt acaggtttac ttgtgagcgg ataacaatat 3541 agtgtgtgga attgtgagcg gataacaatt tactagagaa agaggagaaa tactagatgg 3601 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 3661 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 3721 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 3781 tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc 3841 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 3901 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 3961 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4021 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 4081 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4141 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4201 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 4261 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat 4321 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 4381 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 4441 cctttctgcg tttata //