LOCUS       BBa_T9003               1603 bp    DNA     linear       25-Nov-2024
DEFINITION  lacZalpha protein generator controlled by 3OC6HSL receiver device
ACCESSION   BBa_T9003
VERSION     BBa_T9003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     misc_feature    1088..1102
                     /label=lacZ gene fragment
     CDS             1088..1441
                     /label=lacZ alpha
     promoter        1113..1122
                     /label=T3 promoter
     promoter        1260..1278
                     /label=T7 promoter
     misc_feature    1165..1165
                     /label=C
     misc_feature    1168..1168
                     /label=T
     misc_feature    1174..1174
                     /label=A
     misc_feature    1195..1195
                     /label=C
     misc_feature    1198..1198
                     /label=G
     misc_feature    1286..1435
                     /label=lacZ gene fragment
     misc_feature    1435..1435
                     /label=inserted T
     misc_feature    1442..1466
                     /label=barcode
     misc_feature    1088..1466
                     /label=BBa_E0035
     stem_loop       1486..1529
                     /label=stem_loop
     misc_feature    1475..1554
                     /label=BBa_B0010
     terminator      1475..1554
                     /label=BBa_B0010
     stem_loop       1570..1589
                     /label=T7 TE
     misc_feature    1596..1596
                     /label=stop
     polyA_site      1590..1603
                     /label=polya
     misc_feature    1563..1603
                     /label=BBa_B0012
     terminator      1563..1603
                     /label=BBa_B0012
     terminator      1475..1603
                     /label=BBa_B0015
     misc_feature    1070..1603
                     /label=BBa_E0435
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     1141 ctggagctcc accgcggtgg cggcagcact agagctagtg gatcccccgg gctgtagaaa
     1201 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     1261 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     1321 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1381 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaattaata
     1441 acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct
     1501 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//