BBa_T9010 1 BBa_T9010 aacC2 with RBS 2016-08-21T11:00:00Z 2016-08-22T11:02:40Z Escherichia coli R-Plasmid. Sequence from Genbank: http://www.ncbi.nlm.nih.gov/nuccore/X54723 Translational unit including aacC2 gene encoding gentamicin resistance (BBa_T9000) with strong RBS BBa_B0034 false false _1_ 33565 33565 9 false Changed TAG stop codon to TAA false Abigail Sison component2481504 1 BBa_B0034 component2481508 1 BBa_T9000 annotation2481508 1 BBa_T9000 range2481508 1 19 879 annotation2481504 1 BBa_B0034 range2481504 1 1 12 BBa_T9000 1 BBa_T9000 aacC2 2016-08-21T11:00:00Z 2016-08-22T10:11:14Z Escherichia coli R-Plasmid. Sequence from Genbank: http://www.ncbi.nlm.nih.gov/nuccore/X54723 E.coli R-plasmid aacC2 gene for aminoglycoside-(3)-N-acetyltransferase activity AKA Gentamicin resistance false false _1_ 33565 33565 9 false Changed TAG stop codon to TAA false Abigail Sison annotation2481500 1 ATG Start Codon range2481500 1 1 3 annotation2481501 1 aacC2 coding region range2481501 1 1 861 annotation2481502 1 TAA Stop Codon range2481502 1 859 861 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_T9000_sequence 1 atgcatacgcggaaggcaataacggaggcgcttcaaaaactcggagtccaaaccggtgacctattgatggtgcatgcctcacttaaagcgattggtccggtcgaaggaggagcggagacggtcgttgccgcgttacgctccgcggttgggccgactggcactgtgatgggatacgcatcgtgggaccgatcaccctacgaggagactcgtaatggcgctcggttggatgacaaaacccgccgtacctggccgccgttcgatcccgcaacggccgggacttaccgtgggttcggcctgctgaatcagtttctggttcaagcccccggcgcgcggcgcagcgcgcaccccgatgcatcgatggtcgcggttggtccactggctgaaacgctgacggagcctcacaagctcggtcacgccttgggggaagggtcgcccgtcgagcggttcgttcgccttggcgggaaggccctgctgttgggtgcgccgctaaactccgttaccgcattgcactacgccgaggcggttgccgatatccccaacaaacggcgggtgacgtatgagatgccgatgcttggaagcaacggcgaagtcgcctggaaaacggcatcggattacgattcaaacggcattctcgattgctttgctatcgaaggaaagccggatgcggtcgaaactatagcaaatgcttacgtgaagctcggtcgccatcgagaaggtgtcgtgggctttgctcagtgctacctgttcgacgcgcaggacatcgtgacgttcggcgtcacctatcttgagaagcatttcggaaccactccgatcgtgccagcacacgaagtcgccgagtgctcttgcgagccttcaggttaa BBa_T9010_sequence 1 aaagaggagaaatactagatgcatacgcggaaggcaataacggaggcgcttcaaaaactcggagtccaaaccggtgacctattgatggtgcatgcctcacttaaagcgattggtccggtcgaaggaggagcggagacggtcgttgccgcgttacgctccgcggttgggccgactggcactgtgatgggatacgcatcgtgggaccgatcaccctacgaggagactcgtaatggcgctcggttggatgacaaaacccgccgtacctggccgccgttcgatcccgcaacggccgggacttaccgtgggttcggcctgctgaatcagtttctggttcaagcccccggcgcgcggcgcagcgcgcaccccgatgcatcgatggtcgcggttggtccactggctgaaacgctgacggagcctcacaagctcggtcacgccttgggggaagggtcgcccgtcgagcggttcgttcgccttggcgggaaggccctgctgttgggtgcgccgctaaactccgttaccgcattgcactacgccgaggcggttgccgatatccccaacaaacggcgggtgacgtatgagatgccgatgcttggaagcaacggcgaagtcgcctggaaaacggcatcggattacgattcaaacggcattctcgattgctttgctatcgaaggaaagccggatgcggtcgaaactatagcaaatgcttacgtgaagctcggtcgccatcgagaaggtgtcgtgggctttgctcagtgctacctgttcgacgcgcaggacatcgtgacgttcggcgtcacctatcttgagaagcatttcggaaccactccgatcgtgccagcacacgaagtcgccgagtgctcttgcgagccttcaggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z