BBa_E0430 1 BBa_E0430 EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015) 2004-03-15T12:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 Standard YFP Output Device -LVA tag false true _1_ 0 24 7 In stock false true Caitlin Conboy component942887 1 BBa_E0030 component942885 1 BBa_B0034 component942892 1 BBa_B0010 component942902 1 BBa_B0012 annotation942902 1 BBa_B0012 range942902 1 838 878 annotation942885 1 BBa_B0034 range942885 1 1 12 annotation942892 1 BBa_B0010 range942892 1 750 829 annotation942887 1 BBa_E0030 range942887 1 19 741 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0030 1 eyfp enhanced yellow fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Modificaitons to Clontech EYFP by Reshma Shetty Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J69121 1 BBa_J69121 3OC12-HSL Receiver 2010-08-31T11:00:00Z 2015-05-08T01:08:18Z Gibson Assembly 3OC12-HSL Receiver with GFP [lasR:(p)luxI:GFP] false false _101_ 0 740 101 Not in stock false NA false James Brown BBa_T9201 1 BBa_T9201 C12 Receiver Test Device: YFP 2011-10-31T12:00:00Z 2015-05-08T01:14:53Z NA NA false true _101_ 0 740 101 Not in stock false NA false James Brown component2162026 1 BBa_E0430 component2162016 1 BBa_J69121 annotation2162016 1 BBa_J69121 range2162016 1 1 998 annotation2162026 1 BBa_E0430 range2162026 1 1007 1884 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_E0430_sequence 1 aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_E0030_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_T9201_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttatgagctcgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J69121_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttatgagctcgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z