BBa_V1011 1 GH371 E. coli strain GH371; 5-fluorouracil resistant upp mutant of GeneHogs 2006-06-28T11:00:00Z 2015-05-08T01:14:53Z Generated by lambda-red recombination of a PCR product amplified with oligonucleotides ca371F and ca371R from pKD13 in GeneHogs transformed with pKD46. See Datsenko&Wanner for more information on the methodology: [[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abstract&list_uids=10829079&query_hl=8&itool=pubmed_docsum]] GeneHogs is a phage-resistant variant of DH10B (see [[www.invitrogen.com/content/sfs/manuals/genehogs_man.pdf]]. GH371 is an upp mutant of GeneHogs generated by lambda-red recombination. GH371 is resistant to ~10 ug/mL 5-Fluorouracil and has been used for negative genetic selections (See Chin and coworkers: [[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abstract&list_uids=16408021&query_hl=5&itool=pubmed_docsum]] false false _95_ 0 483 95 No part sequence false N/A false John Anderson igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z