BBa_V1022 1 BBa_V1022 E. coli strain BW20767 2006-11-19T12:00:00Z 2015-05-08T01:14:53Z Barry Wanner Barry Wanner E. coli strain useful for the maintanence of plasmids with the R6K origin of relication and conjugal transfer of plasmids with an RP4 origin of transfer. These properties are conferred by the pir+ gene and a oriT-deficient copy of the RP4 plasmid. Strain principly used as the donor strain for transposon mutagenesis. '''Genotype:''' RP4-2-tet:Mu-1kan::Tn7 integrant leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 gusA (DMluI)::pir?? thi false false _95_ 0 483 95 No part sequence false N/A false John Anderson igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z