BBa_Y00012 1 BBa_Y00012 mitochondrial LYS12 2006-07-05T11:00:00Z 2015-05-08T01:14:53Z custom synthesis from DNA 2.0 S. cerevisiae gene LYS12 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=lys12]] is required for the fourth step of lysine biosynthesis. It is normally encoded by the nuclear genome and the protein is imported to the mitochondria where it oxidatively decarboxylates homo-isocitrate to alpha-ketoadipate. Strains deleted for LYS12 are viable but show decreased growth in the absence of lysine. Part Y00012 is the S. cerevisiae LYS12 gene that has been recoded for expression in the mitochondria, which uses a different genetic code and separate transcription/translation machinery for expression mitochondrial genes. The part is start to stop flanked by biobrick ends so has no promoter for expression or sequences for recombination into the mitochondria. false false _43_ 0 314 1 Not in stock false codon optimized for expression in S. cerevisiae mitochondria, keeping all biobrick sites out of coding region false Natalie Kuldell annotation1883576 1 prefix range1883576 1 1 30 annotation1883577 1 suffix range1883577 1 1143 1175 BBa_Y00012_sequence 1 gtttcttcgaattcgcggccgcttctagagatgtttcgtagtgtagctctgagattaagcgcatgtcgtggtttggcatcgaatgcggccagaaaatcattgacaattgggttaatcccaggtgatggtatcggtaaggaggtgattccggcaggcaaacaagttttggaaaacttaaactccaagcatggtttatcgtttaatttcattgacttgtacgcaggatttcaaacttttcaggaacttgggaaagccttaccagacgagacagtcaaagtgttgaaagaacaatgtcagggcgctttgtttggcgcagttcagtcacctacaacaaaggtcgaaggttattcctcgcctattgttgctttacgtagagaaataggtttgttcgcaaacgtgagaccagtgaaatctgtcgaaggtgaaaagggtaaaccgattgatatagtgatcgtgagagagaatacagaggatttgtatattaagattgaaaaactatatattgacaaagctcttggacttcgtgttgccgatgctacaaaaagaattagcgaaattgctactagaagaattgcgacaatcgcattggatattgctttgaagagattacaaacaagaggtcaagccctgttactggtaactcataagtccaatgtgttaagtcagtctgacgggttattcagggaaatctgcaaagaagtttatgaatctaataaagataagtacggtcaaatcaagtacaacgaacaaatcgttgattctatagtatatagattattcagagaaccccaatgcttcgatgttattgtcgctcctaacttatatggtgatattttgtctgatggagctgctgctttagtaggctcattgggtgtcgtaccatcagccaatgttggtcctgaaattgttattggtgaaccatgccacggctctgcccctgatattgcaggtaaggggattgctaatccgattgctaccattcgtagcactgcattaatgttagagttcttaggtcataacgaggctgcacaagatatctataaagcggtagatgccaatttgcgtgaaggatccatcaaactgcccgatttgggtggaaaggctagtctacaacaggttgttgatgatgtattgagtagattgtagtactagtagcggccgctgcaggaagaaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z