LOCUS       BBa_Z0120               1707 bp    DNA     linear       30-Dec-2025
DEFINITION  T7.2 gene 4A/4B
ACCESSION   BBa_Z0120
VERSION     BBa_Z0120.1  
FEATURES             Location/Qualifiers
     CDS             1..1701
                     /label=gene 4A
     CDS             190..1701
                     /label=gene 4B
     CDS             71..193
                     /label=gene 4.1
     CDS             1424..1762
                     /label=gene 4.2
     promoter        574..637
                     /label=S4: possible promoter
     promoter        1080..1114
                     /label=phi4c
     misc_feature    70..72
                     /label=gat->gac 4.1 start
     misc_feature    1423..1425
                     /label=cat->CAC 4.2 start
     misc_feature    586..588
                     /label=ttt->TTC S4 -35
     misc_feature    610..612
                     /label=tta->CUG S4-10
     misc_feature    1078..1116
                     /label=codon shuffled (13 changes) phi4c
     misc_feature    4..39
                     /label=gac...tac -> gat ... tat codon shuffle
     misc_feature    1702..1707
                     /label=added ngoMIV
ORIGIN
        1 atggataata gtcatgatag cgatagcgtt tttttgtatc acattccttg tgacaactgt
       61 gggagtagtg acgggaactc gctgttctct gacggacaca cgttctgcta cgtatgcgag
      121 aagtggactg ctggtaatga agacactaaa gagagggctt caaaacggaa accctcagga
      181 ggtaaaccaa tgacttacaa cgtgtggaac ttcggggaat ccaatggacg ctactccgcg
      241 ttaactgcga gaggaatctc caaggaaacc tgtcagaagg ctggctactg gattgccaaa
      301 gtagacggtg tgatgtacca agtggctgac tatcgggacc agaacggcaa cattgtgagt
      361 cagaaggttc gagataaaga taagaacttt aagaccactg gtagtcacaa gagtgacgct
      421 ctgttcggga agcacttgtg gaatggtggt aagaagattg tcgttacaga aggtgaaatc
      481 gacatgctta ccgtgatgga acttcaagac tgtaagtatc ctgtagtgtc gttgggtcac
      541 ggtgcctctg ccgctaagaa gacatgcgct gccaactacg aatacttcga tcagttcgaa
      601 cagattatcc tgatgttcga tatggacgaa gcagggcgca aagcagtcga agaggctgca
      661 caggttctac ctgctggtaa ggtacgagtg gcagttcttc cgtgtaagga tgcaaacgag
      721 tgtcacctaa atggtcacga ccgtgaaatc atggagcaag tgtggaatgc tggtccttgg
      781 attcctgatg gtgtggtatc ggctctttcg ttacgtgaac gaatccgtga gcacctatcg
      841 tccgaggaat cagtaggttt acttttcagt ggctgcactg gtatcaacga taagacctta
      901 ggtgcccgtg gtggtgaagt cattatggtc acttccggtt ccggtatggg taagtcaacg
      961 ttcgtccgtc aacaagctct acaatggggc acagcgatgg gcaagaaggt aggcttagcg
     1021 atgcttgagg agtccgttga ggagaccgct gaggacctta taggtctaca caaccgtgtt
     1081 cgtctgcgtc agtctgactc tctgaaacgt gaaatcattg agaacggtaa gttcgaccaa
     1141 tggttcgatg aactgttcgg caacgatacg ttccatctat atgactcatt cgccgaggct
     1201 gagacggata gactgctcgc taagctggcc tacatgcgct caggcttggg ctgtgacgta
     1261 atcattctag accacatctc aatcgtcgta tccgcttctg gtgaatccga tgagcgtaag
     1321 atgattgaca acctgatgac caagctcaaa gggttcgcta agtcaactgg ggtggtgctg
     1381 gtcgtaattt gtcaccttaa gaacccagac aaaggtaaag cacacgagga aggtcgcccc
     1441 gtttctatta ctgacctacg tggttctggc gcactacgcc aactatctga tactattatt
     1501 gcccttgagc gtaatcagca aggcgatatg cctaaccttg tcctcgttcg tattctcaag
     1561 tgccgcttta ctggtgatac tggtatcgct ggctacatgg aatacaacaa ggaaaccgga
     1621 tggcttgaac catcaagtta ctcaggggaa gaagagtcac actcagagtc aacagactgg
     1681 tccaacgaca ctgacttcta agccggc
//