BBa_Z0120 1 BBa_Z0120 T7.2 gene 4A/4B 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z The full-length gene 4 protein is a 5??->3?? helicase of the E. coli DnaB family and a DNA primase. An in-frame internal start site within gene 4 results in a protein, gp4B, that lacks the N-terminal 63 amino acids of the 63 kDa gp4A, and consequently lacks primase activity. The primase recognition sites on ssDNA are 5??-G/TGGTC, and 5??-GTGTC. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1862191 1 cat->CAC 4.2 start range1862191 1 1423 1425 annotation1849942 1 gene 4A range1849942 1 1 1701 annotation1862193 1 tta->CUG S4-10 range1862193 1 610 612 annotation1883672 1 added ngoMIV range1883672 1 1702 1707 annotation1849946 1 gene 4B range1849946 1 190 1701 annotation1881252 1 gac...tac -> gat ... tat codon shuffle range1881252 1 4 39 annotation1849947 1 gene 4.1 range1849947 1 71 193 annotation1862192 1 ttt->TTC S4 -35 range1862192 1 586 588 annotation1849949 1 S4: possible promoter range1849949 1 574 637 annotation1849948 1 gene 4.2 range1849948 1 1424 1762 annotation1849950 1 phi4c range1849950 1 1080 1114 annotation1862194 1 codon shuffled (13 changes) phi4c range1862194 1 1078 1116 annotation1862190 1 gat->gac 4.1 start range1862190 1 70 72 BBa_Z0120_sequence 1 atggataatagtcatgatagcgatagcgtttttttgtatcacattccttgtgacaactgtgggagtagtgacgggaactcgctgttctctgacggacacacgttctgctacgtatgcgagaagtggactgctggtaatgaagacactaaagagagggcttcaaaacggaaaccctcaggaggtaaaccaatgacttacaacgtgtggaacttcggggaatccaatggacgctactccgcgttaactgcgagaggaatctccaaggaaacctgtcagaaggctggctactggattgccaaagtagacggtgtgatgtaccaagtggctgactatcgggaccagaacggcaacattgtgagtcagaaggttcgagataaagataagaactttaagaccactggtagtcacaagagtgacgctctgttcgggaagcacttgtggaatggtggtaagaagattgtcgttacagaaggtgaaatcgacatgcttaccgtgatggaacttcaagactgtaagtatcctgtagtgtcgttgggtcacggtgcctctgccgctaagaagacatgcgctgccaactacgaatacttcgatcagttcgaacagattatcctgatgttcgatatggacgaagcagggcgcaaagcagtcgaagaggctgcacaggttctacctgctggtaaggtacgagtggcagttcttccgtgtaaggatgcaaacgagtgtcacctaaatggtcacgaccgtgaaatcatggagcaagtgtggaatgctggtccttggattcctgatggtgtggtatcggctctttcgttacgtgaacgaatccgtgagcacctatcgtccgaggaatcagtaggtttacttttcagtggctgcactggtatcaacgataagaccttaggtgcccgtggtggtgaagtcattatggtcacttccggttccggtatgggtaagtcaacgttcgtccgtcaacaagctctacaatggggcacagcgatgggcaagaaggtaggcttagcgatgcttgaggagtccgttgaggagaccgctgaggaccttataggtctacacaaccgtgttcgtctgcgtcagtctgactctctgaaacgtgaaatcattgagaacggtaagttcgaccaatggttcgatgaactgttcggcaacgatacgttccatctatatgactcattcgccgaggctgagacggatagactgctcgctaagctggcctacatgcgctcaggcttgggctgtgacgtaatcattctagaccacatctcaatcgtcgtatccgcttctggtgaatccgatgagcgtaagatgattgacaacctgatgaccaagctcaaagggttcgctaagtcaactggggtggtgctggtcgtaatttgtcaccttaagaacccagacaaaggtaaagcacacgaggaaggtcgccccgtttctattactgacctacgtggttctggcgcactacgccaactatctgatactattattgcccttgagcgtaatcagcaaggcgatatgcctaaccttgtcctcgttcgtattctcaagtgccgctttactggtgatactggtatcgctggctacatggaatacaacaaggaaaccggatggcttgaaccatcaagttactcaggggaagaagagtcacactcagagtcaacagactggtccaacgacactgacttctaagccggc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z