LOCUS       BBa_Z0129               2389 bp    DNA     linear       01-Jun-2024
DEFINITION  T7.2 gene 12
ACCESSION   BBa_Z0129
VERSION     BBa_Z0129.1  
FEATURES             Location/Qualifiers
     promoter        337..399
                     /label=F2
     misc_feature    382..384
                     /label=gtt->GTG F2-35
     misc_feature    4..39
                     /label=gca...ggt->gca...ggc codon shuffle
     misc_feature    2386..2389
                     /label=added ttaa to form PacI
ORIGIN
        1 atggcactga tatcccaaag cattaaaaat ttaaaaggcg gtatcagcca acagcctgac
       61 atccttcgtt atccagacca agggtcacgc caagttaacg gttggtcttc ggagaccgag
      121 ggcctccaaa agcgtccacc tcttgttttc ttaaatacac ttggagacaa cggtgcgtta
      181 ggtcaagctc cgtacatcca cctgattaac cgagatgagc acgaacagta ttacgctgtg
      241 ttcactggta gcggaatccg agtgttcgac ctttctggta acgagaagca agttaggtat
      301 cctaacggtt ccaactacat caagaccgct aatccacgta acgacctgcg aatggttact
      361 gtagcagact atacgttcat cgtgaaccgt aacgttgttg cacagaagaa cacaaagtct
      421 gtcaacttac cgaattacaa ccctaatcaa gacggattga ttaacgttcg tggtggtcag
      481 tatggtaggg aactaattgt acacattaac ggtaaagacg ttgcgaagta taagatacca
      541 gatggtagtc aacctgaaca cgtaaacaat acggatgccc aatggttagc tgaagagtta
      601 gccaagcaga tgcgcactaa cttgtctgat tggactgtaa atgtagggca agggttcatc
      661 catgtgaccg cacctagtgg tcaacagatt gactccttca cgactaaaga tggctacgca
      721 gaccagttga ttaaccctgt gacccactac gctcagtcgt tctctaagct gccacctaat
      781 gctcctaacg gctacatggt gaaaatcgta ggggacgcct ctaagtctgc cgaccagtat
      841 tacgttcggt atgacgctga gcggaaagtt tggactgaga ctttaggttg gaacactgag
      901 gaccaagttc tatgggaaac catgccacac gctcttgtgc gagccgctga cggtaatttc
      961 gacttcaagt ggcttgagtg gtctcctaag tcttgtggtg acgttgacac caacccttgg
     1021 ccttcttttg ttggttcaag tattaacgat gtgttcttct tccgtaaccg cttaggattc
     1081 cttagtgggg agaacatcat attgagtcgt acagccaaat acttcaactt ctaccctgcg
     1141 tccattgcga accttagtga tgacgaccct atagacgtag ctgtgagtac caaccgaata
     1201 gcaatcctta agtacgccgt tccgttctca gaagagttac tcatctggtc cgatgaagca
     1261 caattcgtcc tgactgcctc gggtactctc acatctaagt cggttgagtt gaacctaacg
     1321 acccagtttg acgtacagga ccgagcgaga ccttttggga ttgggcgtaa tgtctacttt
     1381 gctagtccga ggtccagctt cacgtccatc cacaggtact acgctgtgca ggatgtcagt
     1441 tccgttaaga atgctgagga cattacatca cacgttccta actacatccc taatggtgtg
     1501 ttcagtattt gcggaagtgg tacggaaaac ttctgttcgg tactatctca cggggaccct
     1561 agtaaaatct tcatgtacaa attcctgtac ctgaacgaag agttaaggca acagtcgtgg
     1621 tctcattggg actttgggga aaacgtacag gttctagctt gtcagagtat cagctcagat
     1681 atgtatgtga ttcttcgcaa tgagttcaat acgttcctag ctagaatctc tttcactaag
     1741 aacgccattg acttacaggg agaaccctat cgtgccttta tggacatgaa gattcgatac
     1801 acgattccta gtggaacata caacgatgac acattcacta cctctattca tattccaaca
     1861 atttatggtg caaacttcgg gaggggcaaa atcactgtat tggagcctga tggtaagata
     1921 accgtgtttg agcaacctac ggctgggtgg aatagcgacc cttggctgag actcagcggt
     1981 aacttggagg gacgcatggt gtacattggg ttcaacatta acttcgtata tgagttctct
     2041 aagttcctca tcaagcagac tgccgacgac gggtctacct ccacggaaga cattgggcgc
     2101 ttacagttac gccgagcgtg ggttaactac gagaactctg gtacgtttga catttatgtt
     2161 gagaaccaat cgtctaactg gaagtacaca atggctggtg cccgattagg ctctaacact
     2221 ctgagggctg ggagactgaa cttagggacc ggacaatatc gattccctgt ggttggtaac
     2281 gccaagttca acactgtata catcttgtca gatgagacta cccctctgaa catcattggg
     2341 tgtggctggg aaggtaacta cttacggaga agttccggta tttaattaa
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