##gff-version 3
##sequence-region BBa_Z0144 1 437
BBa_Z0144	.	sequence_alteration	434	437	.	+	0	ID=annotation1883680;Name=added CATGC to form SphI
BBa_Z0144	.	point_mutation	7	15	.	+	0	ID=annotation1862600;Name=gaattttta->gagttcctc E-10
BBa_Z0144	.	CDS	116	367	.	+	0	ID=annotation1855761;Name=gene 18.7
BBa_Z0144	.	promoter	1	34	.	+	0	ID=annotation1862602;Name=E promoter remnant
BBa_Z0144	.	promoter	8	13	.	+	0	ID=annotation1862601;Name=E -10 region
>BBa_Z0144
atgctagagttcctccgtaagctaatcccttgggttctcgctgggatgctattcgggttaggatggcatctagggtcag
actcaatggacgctaaatggaaacaggaggtacacaatgagtacgttaagagagttgaggctgcgaagagcactcaaag
agcaatcgatgcggtatctgctaagtatcaagaagaccttgccgcgctggaagggagcactgataggattatttctgat
ttgcgtagcgacaataagcggttgcgcgtcagagtcaaaactaccggaacctccgatggtcagtgtggattcgagcctg
atggtcgagccgaacttgacgaccgagatgctaaacgtattctcgcagtgacccagaagggtgacgcatggattcgtgc
gttacaggatactattcgtgaactgcaacgtaagtagcatgc