LOCUS       BBa_Z0152                699 bp    DNA     linear       01-Nov-2024
DEFINITION  T7.2 gene 2.5
ACCESSION   BBa_Z0152
VERSION     BBa_Z0152.1  
FEATURES             Location/Qualifiers
     misc_feature    690..690
                     /label=a->G mutation to remove RBS
     promoter        3..66
                     /label=S2: possible promoter
     promoter        16..20
                     /label=S2 -35 region
     promoter        40..45
                     /label=S2 -10 region
     misc_feature    16..18
                     /label=ttc -> TTT
     misc_feature    4..39
                     /label=ggc...gct->ggc..gct codon shuffle
ORIGIN
        1 atggcgaaaa aaatatttac aagtgcgtta ggaacagctg aaccttacgc ttacatcgcc
       61 aagccggact acggcaacga agagcgtggc tttgggaacc ctcgtggtgt ctataaagtt
      121 gacctgacta ttcccaacaa agacccgcgc tgccagcgta tggtcgatga aatcgtgaag
      181 tgtcacgaag aggcttatgc tgctgccgtt gaggaatacg aagctaatcc acctgctgta
      241 gctcgtggta agaaaccgct gaaaccgtat gagggtgaca tgccgttctt cgataacggt
      301 gacggtacga ctacctttaa gttcaaatgc tacgcgtctt tccaagacaa gaagaccaaa
      361 gagaccaagc acatcaatct ggttgtggtt gactcaaaag gtaagaagat ggaagacgtt
      421 ccgattatcg gtggtggctc taagctgaaa gttaaatatt ctctggttcc atacaagtgg
      481 aacactgctg taggtgcgag cgttaagctg caactggaat ccgtgatgct ggtcgaactg
      541 gctacctttg gtggcggtga agacgattgg gctgacgaag ttgaagagaa cggctatgtt
      601 gcctctggtt ctgccaaagc gagcaaacca cgcgacgaag aaagctggga cgaagacgac
      661 gaagagtccg aggaagcaga cgaagacggg gacttctaa
//