BBa_Z0106 1 BBa_Z0106 T7.2 gene 0.7 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation Gene 0.7 codes for a protein that inactivates host-catalyzed transcription, it is also a serine-threonine protein kinase. The two activities are separable. Gp0.7 phosphorylates many host proteins, including ribosome components, ribonucleases III and E, and the β and β?? subunits of E. coli RNAP. The BR3 and Y49 strains contain the rpoC-E1158K mutation, which renders gp0.7 essential for phage growth. The mutation affects the region of β?? to which the T7 protein gp2 binds. Gene 0.7 is non-essential in wild-type hosts and is actually detrimental when T7 is grown at 30˚C in rich media. Early T7 RNA and protein synthesis is prolonged after infection by gp0.7 mutants. However, phage growth at elevated temperatures or in minimal media, or in cells harboring the Col Ib plasmid renders gene 0.7 essential. The processes underlying the conditional necessity for gp0.7 function have not been determined. The E. coli C promoter -35 region is located on the 3' end of the coding sequence. Removing the downstream region will probably be sufficient to delete functioning of the promoter. false false _10_ 0 250 10 Not in stock false false T7.2 annotation1883644 1 added BsiWI range1883644 1 1081 1086 annotation1476456 1 -45 region of C promoter range1476456 1 1049 1049 annotation1476455 1 -35 region of C promoter range1476455 1 1058 1062 BBa_Z0133 1 BBa_Z0133 T7.2 gene 16 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Internal core protein of phage capsid; ~4 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855274 1 S6: possible promoter range1855274 1 3876 3939 annotation1862596 1 ttgact->CTGACC S6-35 range1862596 1 3889 3894 annotation1862595 1 aagatttat->AAAATCTAC S6-10 range1862595 1 3910 3918 BBa_Z0158 1 BBa_Z0158 T7.2 gene 0.3 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z 3' end of gene mutated to remove gene 0.4 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1883643 1 add apaI range1883643 1 355 360 annotation1855703 1 t->C mutation range1855703 1 345 345 BBa_Z0125 1 BBa_Z0125 T7.2 gene 8 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Head-tail connector. 12 copies per virion. Three possible host promoters with -35 regions at 293, 1100, 1249 false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1883674 1 added SalI range1883674 1 1612 1617 annotation1855239 1 possible promoter site? range1855239 1 1425 1425 BBa_Z0108 1 BBa_Z0108 T7.2 gene 1.1 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation open reading frame for gene 1.1. from wild-type T7 genome false false _10_ 0 250 10 Not in stock false false T7.2 BBa_Z0157 1 BBa_Z0157 T7.2 gene 17.5 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z Mutations on 3' end to remove gene 18 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855282 1 g->A mutation range1855282 1 198 198 annotation1855281 1 g->A mutation range1855281 1 201 201 BBa_Z0160 1 BBa_Z0160 T7.2 gene 5.5-5.7 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z Removes the overlap of gene 5.9 RBS. Not the actual sequence in the design of T7.1, allows the frameshift again. false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1862272 1 gene 5.7 range1862272 1 300 509 annotation1855777 1 a->C mutation range1855777 1 497 497 annotation1855776 1 a->G mutation range1855776 1 500 500 annotation1862271 1 gene 5.5 range1862271 1 1 300 annotation1862270 1 gene 5.5-5.7 fusion range1862270 1 1 509 BBa_Z0124 1 BBa_Z0124 T7.2 gene 7.3 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Gene 7 was originally defined by mutations that mapped between genes 6 and 8 but was subsequently shown to be two genes, 7 and 7.3. Gp7.3 is a tail protein, present in ~30 copies per particle, that is essential for infectivity though not particle formation. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855232 1 possible promoter site? range1855232 1 168 168 annotation1883678 1 added bstEII range1883678 1 301 307 annotation1883626 1 att->ATC range1883626 1 295 297 BBa_Z0128 1 BBa_Z0128 T7.2 gene 11 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z structural tail protein of phage capsid; 12 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881255 1 cgc...gag ->cga...gaa codon shuffle range1881255 1 4 39 BBa_Z0139 1 BBa_Z0139 T7.2 gene 4.5 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z conserved; non-essential false false _11_ 0 64 11 Not in stock false false Sriram Kosuri BBa_Z0273 1 BBa_Z0273 T7 R0.3 RNAseIII site 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Contains 9 bp before and 10 bp after stem loop(up to start of 0.3 RBS). false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875207 1 R0.3 stem loop range1875207 1 10 58 BBa_Z0253 1 BBa_Z0253 T7 weak binding promoter 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z -- No description -- false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862684 1 ag->TA from concensus range1862684 1 27 28 annotation1862691 1 ag->GA from concensus range1862691 1 31 32 BBa_Z0272 1 BBa_Z0272 T7.2 Tphi transcriptional terminator 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Taken 10 bp before expected start of stem loop structure, and 3bp after expected stop of transcription (7bp after stem loop) false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862785 1 terminator stem loop range1862785 1 11 46 BBa_Z0119 1 BBa_Z0119 T7.2 gene 3.5 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z Gene 3.5 codes for lysozyme, which is actually not a lysozyme but an N-acetylmuramyl-L-alanine amidase. The most important functions of lysozyme are also not in cell lysis but lie in controlling initiation and termination by T7 RNAP, which in turn affect DNA replication and packaging. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1849938 1 S3: possible promoter range1849938 1 110 173 annotation1860957 1 S3 -10 region range1860957 1 147 152 annotation1862116 1 ggt???>GGC range1862116 1 121 123 annotation1860956 1 S3 -35 region range1860956 1 123 127 annotation1862117 1 ttt->TTC range1862117 1 145 147 BBa_Z0151 1 BBa_Z0151 T7.2 gene 1.7 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z Bases have been changed to remove gene 1.8 start site acataaAgataaatgttaCgcataa false false _10_ 0 64 11 Not in stock false false Sriram Kosuri annotation1849888 1 g->A to remove gene 1.8 RBS range1849888 1 573 573 annotation1849887 1 t->C to remove gene 1.8 start range1849887 1 585 585 BBa_Z0148 1 BBa_Z0148 T7.2 gene 19.5 2006-05-03T11:00:00Z 2015-05-08T01:14:54Z non-essential; conserved false false _11_ 0 64 11 Not in stock false false Sriram Kosuri BBa_Z0121 1 BBa_Z0121 T7.2 gene 5 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z T7 DNA Polymerase consists of two proteins, the gene 5 protein and the processivity factor E. coli thioredoxin. Thioredoxin forms a 1:1 complex with the T7 protein and increases its processivity by at least 1000-fold, the complex also increases the activity of the associated 5??->3?? exonuclease on dsDNA but has little effect on ssDNA exonucleolytic activity. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881253 1 atc...gag -> atc...gaa codon shuffle range1881253 1 4 39 annotation1883673 1 added pstI range1883673 1 2116 2121 BBa_Z0144 1 BBa_Z0144 T7.2 gene 18.5 (18.7) 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z lysis associated protein. Homologs of 18.5/18.7 are found in all phages (in T1 the two proteins are fused); contains gene 18.7 within coding domain. false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1883680 1 added CATGC to form SphI range1883680 1 434 437 annotation1855761 1 gene 18.7 range1855761 1 116 367 annotation1862602 1 E promoter remnant range1862602 1 1 34 annotation1862600 1 gaattttta->gagttcctc E-10 range1862600 1 7 15 annotation1862601 1 E -10 region range1862601 1 8 13 BBa_Z0116 1 BBa_Z0116 T7.2 gene 2 2006-04-11T11:00:00Z 2015-05-08T01:14:54Z Gp2 binds to and is an inhibitor of E. coli RNAP but in K-12 or B strains the defects due to a lack of gene 2 activity are reduced DNA replication and premature breakdown of replicating DNA, specifically at the left genome end where the major E. coli promoters are found. false false _10_ 0 64 11 Not in stock false false Sri Kosuri annotation1883670 1 added ecoRI range1883670 1 196 201 annotation1881250 1 tca...aat->agc...aat range1881250 1 4 39 BBa_Z0115 1 BBa_Z0115 T7.2 gene 1.2 2006-04-11T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation Gene 1.2 inhibits E. coli dGTPase and converts it into a rGTP-binding protein, which is important for T7 only when dgt is over-expressed (optA1 mutation). T7 gene 1.2 also causes phage exclusion by the F plasmid, but T3 gene 1.2 overcomes the exclusion system. false false _10_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0137 1 BBa_Z0137 T7.2 gene 19 (19.2, 19.3) 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z large terminase subunit. binds capsid pro-head. Also contains within the sequence for 19.2 and 19.3 within the gene. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855298 1 gene 19.2 range1855298 1 647 904 annotation1855299 1 gene 19.3 range1855299 1 1184 1357 annotation1881265 1 tct...gcg -> agc...gct codon shuffle range1881265 1 4 39 BBa_Z0118 1 BBa_Z0118 T7 gene 3 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z Encodes for a endonuclease that prefers ssDNA. It functions along with gene 6 to degrade the host chromosome during infection and provide nucleotides for subsequent phage DNA replication. Gene 3 is also a Holliday junction resolvase. false false _11_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0293 1 BBa_Z0293 T7 Right End 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Right End of T7 DNA. Important in packaging. Contains short terminal repeat region and the right terminal repeat. Contains sequence starting after gene 19.5 stop site. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875190 1 Right Terminal Repeat range1875190 1 240 399 annotation1875189 1 Short Terminal Repeat Region range1875189 1 68 226 BBa_Z0503 1 BBa_Z0503 T7.2 revision 3 2006-06-21T11:00:00Z 2015-05-08T01:14:55Z Designs by Alex Mallet and Sri Kosuri Fixed secondary structure on venus yfp and rbs. false false _11_ 0 64 10 Not in stock false see http://openwetware.org/wiki/T7.2 false Sriram Kosuri component2243055 1 BBa_Z0138 component2243081 1 BBa_Z0273 component2242991 1 BBa_Z0252 component2243036 1 BBa_Z0262 component2243067 1 BBa_Z0121 component2243170 1 BBa_Z0137 component2243166 1 BBa_Z0262 component2243146 1 BBa_Z0251 component2243023 1 BBa_Z0262 component2243101 1 BBa_Z0262 component2242958 1 BBa_Z0261 component2243144 1 BBa_Z0133 component2242957 1 BBa_Z0301 component2243053 1 BBa_Z0253 component2243020 1 BBa_Z0152 component2243057 1 BBa_Z0139 component2243098 1 BBa_Z0125 component2243125 1 BBa_Z0273 component2243035 1 BBa_Z0252 component2242998 1 BBa_Z0271 component2243107 1 BBa_Z0251 component2242971 1 BBa_Z0262 component2243095 1 BBa_Z0262 component2242952 1 BBa_Z0294 component2243174 1 BBa_Z0148 component2242946 1 BBa_Z0253 component2243123 1 BBa_Z0129 component2243090 1 BBa_Z0262 component2242964 1 BBa_Z0262 component2243132 1 BBa_Z0262 component2243112 1 BBa_Z0127 component2243172 1 BBa_Z0251 component2242954 1 BBa_Z0273 component2243088 1 BBa_Z0261 component2243108 1 BBa_Z0261 component2243006 1 BBa_Z0261 component2243001 1 BBa_Z0253 component2243064 1 BBa_Z0261 component2243149 1 BBa_Z0134 component2243031 1 BBa_Z0273 component2243139 1 BBa_Z0132 component2243165 1 BBa_Z0144 component2243118 1 BBa_Z0262 component2243068 1 BBa_Z0261 component2243140 1 BBa_Z0262 component2243056 1 BBa_Z0261 component2243173 1 BBa_Z0261 component2242984 1 BBa_Z0262 component2243074 1 BBa_Z0160 component2243138 1 BBa_Z0262 component2242994 1 BBa_Z0261 component2243089 1 BBa_Z0123 component2243094 1 BBa_Z0124 component2243158 1 BBa_Z0273 component2243054 1 BBa_Z0261 component2243100 1 BBa_Z0251 component2243115 1 BBa_Z0261 component2243009 1 BBa_Z0116 component2242961 1 BBa_Z0158 component2243005 1 BBa_Z0151 component2242986 1 BBa_Z0261 component2243075 1 BBa_Z0261 component2243154 1 BBa_Z0261 component2243159 1 BBa_Z0261 component2243137 1 BBa_Z0156 component2243087 1 BBa_Z0159 component2242993 1 BBa_Z0273 component2242987 1 BBa_Z0115 component2243131 1 BBa_Z0155 component2243059 1 BBa_Z0273 component2243114 1 BBa_Z0272 component2242955 1 BBa_Z0261 component2243012 1 BBa_Z0253 component2243105 1 BBa_Z0126 component2243117 1 BBa_Z0128 component2243063 1 BBa_Z0252 component2243084 1 BBa_Z0262 component2243177 1 BBa_Z0293 component2243002 1 BBa_Z0262 component2243029 1 BBa_Z0119 component2243153 1 BBa_Z0157 component2243013 1 BBa_Z0262 component2243156 1 BBa_Z0136 component2243079 1 BBa_Z0154 component2243083 1 BBa_Z0251 component2242943 1 BBa_Z0292 component2243022 1 BBa_Z0118 component2243147 1 BBa_Z0262 component2242983 1 BBa_Z0291 component2243127 1 BBa_Z0251 component2242970 1 BBa_Z0273 component2243021 1 BBa_Z0262 component2242985 1 BBa_Z0108 component2242968 1 BBa_Z0106 component2242979 1 BBa_Z0107 component2242996 1 BBa_Z0109 component2243150 1 BBa_Z0262 component2243128 1 BBa_Z0262 component2242963 1 BBa_Z0273 component2243050 1 BBa_Z0120 annotation2243177 1 BBa_Z0293 range2243177 1 36541 36939 annotation2243125 1 BBa_Z0273 range2243125 1 24390 24457 annotation2243146 1 BBa_Z0251 range2243146 1 31788 31822 annotation2243140 1 BBa_Z0262 range2243140 1 27811 27830 annotation2243001 1 BBa_Z0253 range2243001 1 7789 7823 annotation2243054 1 BBa_Z0261 range2243054 1 12226 12245 annotation2243174 1 BBa_Z0148 range2243174 1 36391 36540 annotation2243009 1 BBa_Z0116 range2243009 1 8455 8655 annotation2243153 1 BBa_Z0157 range2243153 1 33531 33734 annotation2243154 1 BBa_Z0261 range2243154 1 33735 33754 annotation2243068 1 BBa_Z0261 range2243068 1 14993 15012 annotation2243100 1 BBa_Z0251 range2243100 1 19080 19114 annotation2243117 1 BBa_Z0128 range2243117 1 21390 21980 annotation2242987 1 BBa_Z0115 range2242987 1 6285 6542 annotation2242996 1 BBa_Z0109 range2242996 1 6666 7751 annotation2242984 1 BBa_Z0262 range2242984 1 6116 6135 annotation2242970 1 BBa_Z0273 range2242970 1 3210 3277 annotation2243081 1 BBa_Z0273 range2243081 1 16451 16518 annotation2243022 1 BBa_Z0118 range2243022 1 9430 9879 annotation2243127 1 BBa_Z0251 range2243127 1 24458 24492 annotation2243165 1 BBa_Z0144 range2243165 1 34118 34554 annotation2242998 1 BBa_Z0271 range2242998 1 7752 7788 annotation2243149 1 BBa_Z0134 range2243149 1 31843 33510 annotation2242955 1 BBa_Z0261 range2242955 1 916 935 annotation2243087 1 BBa_Z0159 range2243087 1 16574 16828 annotation2243118 1 BBa_Z0262 range2243118 1 21981 22000 annotation2242964 1 BBa_Z0262 range2242964 1 2104 2123 annotation2243144 1 BBa_Z0133 range2243144 1 27831 31787 annotation2243090 1 BBa_Z0262 range2243090 1 17116 17135 annotation2242971 1 BBa_Z0262 range2242971 1 3278 3297 annotation2242986 1 BBa_Z0261 range2242986 1 6265 6284 annotation2242985 1 BBa_Z0108 range2242985 1 6136 6264 annotation2243112 1 BBa_Z0127 range2243112 1 20114 21315 annotation2242991 1 BBa_Z0252 range2242991 1 6543 6577 annotation2243035 1 BBa_Z0252 range2243035 1 10429 10463 annotation2243107 1 BBa_Z0251 range2243107 1 20059 20093 annotation2243036 1 BBa_Z0262 range2243036 1 10464 10483 annotation2243053 1 BBa_Z0253 range2243053 1 12191 12225 annotation2242994 1 BBa_Z0261 range2242994 1 6646 6665 annotation2243055 1 BBa_Z0138 range2243055 1 12246 12458 annotation2243139 1 BBa_Z0132 range2243139 1 25567 27810 annotation2243172 1 BBa_Z0251 range2243172 1 36336 36370 annotation2243158 1 BBa_Z0273 range2243158 1 34030 34097 annotation2243057 1 BBa_Z0139 range2243057 1 12479 12748 annotation2243095 1 BBa_Z0262 range2243095 1 17443 17462 annotation2243166 1 BBa_Z0262 range2243166 1 34555 34574 annotation2243150 1 BBa_Z0262 range2243150 1 33511 33530 annotation2243020 1 BBa_Z0152 range2243020 1 8711 9409 annotation2243132 1 BBa_Z0262 range2243132 1 24930 24949 annotation2243023 1 BBa_Z0262 range2243023 1 9880 9899 annotation2243138 1 BBa_Z0262 range2243138 1 25547 25566 annotation2243128 1 BBa_Z0262 range2243128 1 24493 24512 annotation2243114 1 BBa_Z0272 range2243114 1 21316 21369 annotation2242961 1 BBa_Z0158 range2242961 1 1676 2035 annotation2243063 1 BBa_Z0252 range2243063 1 12817 12851 annotation2243012 1 BBa_Z0253 range2243012 1 8656 8690 annotation2243147 1 BBa_Z0262 range2243147 1 31823 31842 annotation2243083 1 BBa_Z0251 range2243083 1 16519 16553 annotation2243067 1 BBa_Z0121 range2243067 1 12872 14992 annotation2243159 1 BBa_Z0261 range2243159 1 34098 34117 annotation2243075 1 BBa_Z0261 range2243075 1 15522 15541 annotation2243131 1 BBa_Z0155 range2243131 1 24513 24929 annotation2243105 1 BBa_Z0126 range2243105 1 19135 20058 annotation2243101 1 BBa_Z0262 range2243101 1 19115 19134 annotation2243013 1 BBa_Z0262 range2243013 1 8691 8710 annotation2243115 1 BBa_Z0261 range2243115 1 21370 21389 annotation2242968 1 BBa_Z0106 range2242968 1 2124 3209 annotation2243173 1 BBa_Z0261 range2243173 1 36371 36390 annotation2243137 1 BBa_Z0156 range2243137 1 24950 25546 annotation2243098 1 BBa_Z0125 range2243098 1 17463 19079 annotation2242957 1 BBa_Z0301 range2242957 1 936 1655 annotation2243021 1 BBa_Z0262 range2243021 1 9410 9429 annotation2242963 1 BBa_Z0273 range2242963 1 2036 2103 annotation2243084 1 BBa_Z0262 range2243084 1 16554 16573 annotation2243005 1 BBa_Z0151 range2243005 1 7844 8434 annotation2242993 1 BBa_Z0273 range2242993 1 6578 6645 annotation2243089 1 BBa_Z0123 range2243089 1 16849 17115 annotation2242983 1 BBa_Z0291 range2242983 1 5956 6115 annotation2242958 1 BBa_Z0261 range2242958 1 1656 1675 annotation2243074 1 BBa_Z0160 range2243074 1 15013 15521 annotation2242943 1 BBa_Z0292 range2242943 1 1 377 annotation2243029 1 BBa_Z0119 range2243029 1 9900 10360 annotation2243002 1 BBa_Z0262 range2243002 1 7824 7843 annotation2243094 1 BBa_Z0124 range2243094 1 17136 17442 annotation2243050 1 BBa_Z0120 range2243050 1 10484 12190 annotation2243059 1 BBa_Z0273 range2243059 1 12749 12816 annotation2243006 1 BBa_Z0261 range2243006 1 8435 8454 annotation2243088 1 BBa_Z0261 range2243088 1 16829 16848 annotation2243123 1 BBa_Z0129 range2243123 1 22001 24389 annotation2242946 1 BBa_Z0253 range2242946 1 378 412 annotation2243056 1 BBa_Z0261 range2243056 1 12459 12478 annotation2243108 1 BBa_Z0261 range2243108 1 20094 20113 annotation2243079 1 BBa_Z0154 range2243079 1 15542 16450 annotation2242954 1 BBa_Z0273 range2242954 1 848 915 annotation2242979 1 BBa_Z0107 range2242979 1 3298 5955 annotation2243064 1 BBa_Z0261 range2243064 1 12852 12871 annotation2243170 1 BBa_Z0137 range2243170 1 34575 36335 annotation2242952 1 BBa_Z0294 range2242952 1 413 847 annotation2243031 1 BBa_Z0273 range2243031 1 10361 10428 annotation2243156 1 BBa_Z0136 range2243156 1 33755 34029 BBa_Z0291 1 BBa_Z0291 T7.2 primary origin of replication 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z -- No description -- false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862810 1 R1.1 stem loop range1862810 1 28 80 annotation1862808 1 T7 phi1.1A promoter range1862808 1 2 36 annotation1862809 1 T7 phi1.1B promoter range1862809 1 77 111 BBa_Z0155 1 BBa_Z0155 T7.2 gene 13 2006-05-03T11:00:00Z 2015-05-08T01:14:54Z Mutations from wild-type on 3' end to mutate Gene 14 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855259 1 g->C mutation range1855259 1 414 414 annotation1855258 1 g->C mutation range1855258 1 411 411 BBa_Z0107 1 BBa_Z0107 T7.2 gene 1 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation Encodes gp1: T7 RNA polymerase from wild-type T7; Suppressor mutation in 19 allows no gene 1 late in infection. It's thought that gene 1 is hanging out at the CJ terminator at the pause site... recruiting 18 and 19 after packaging. (Studier FW, PC) false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476462 1 S1 -35 site range1476462 1 2498 2502 annotation1881041 1 aac...gac->aat..gat range1881041 1 4 39 annotation1476461 1 S1 -10 site range1476461 1 2520 2525 annotation1476460 1 S1 promoter startsite range1476460 1 2532 2532 annotation1860880 1 gat -> GAC range1860880 1 2518 2520 annotation1883668 1 added BspEI range1883668 1 2653 2658 annotation1860879 1 gttgac -> GTAGAT range1860879 1 2497 2502 BBa_Z0129 1 BBa_Z0129 T7.2 gene 12 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z a structural tail protein of the phage capsid; 6 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881263 1 gca...ggt->gca...ggc codon shuffle range1881263 1 4 39 annotation1883677 1 added ttaa to form PacI range1883677 1 2386 2389 annotation1881262 1 gtt->GTG F2-35 range1881262 1 382 384 annotation1881261 1 F2 range1881261 1 337 399 BBa_Z0271 1 BBa_Z0271 T7.2 TE transcriptional terminator 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Takes 6bp ahead of the expected start of the stem loop, and 3bp after the expected termination site (11bp after end of stem loop). Causes termination by E. coli RNA polymerase (but not T7 RNA polymerase). false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862801 1 TE expected stem loop range1862801 1 7 26 BBa_Z0132 1 BBa_Z0132 T7.2 gene 15 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z internal core protein of phage capsid; ~8 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0292 1 BBa_Z0292 T7 Left End 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Left end of T7 genome. Includes terminal repeat, short repeats, leftward host promoter A0. Contains sequence up to -27bp of T7 promoter phi0L. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875186 1 left terminal repeat range1875186 1 1 160 annotation1875188 1 A0 range1875188 1 210 272 annotation1875187 1 short left terminal repeat region range1875187 1 175 338 BBa_Z0294 1 BBa_Z0294 A1,A2,A3,boxA 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Host promoter region containing 3 promoters and boxA antitermination site. Takes natural sequence from end of phi0L to start of R0.3 false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1883627 1 add gctc to give SacI range1883627 1 432 435 annotation1875191 1 A1 range1875191 1 37 99 annotation1875194 1 boxA antitermination site range1875194 1 400 409 annotation1875192 1 A2 range1875192 1 164 227 annotation1875193 1 A3 range1875193 1 289 351 BBa_Z0120 1 BBa_Z0120 T7.2 gene 4A/4B 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z The full-length gene 4 protein is a 5??->3?? helicase of the E. coli DnaB family and a DNA primase. An in-frame internal start site within gene 4 results in a protein, gp4B, that lacks the N-terminal 63 amino acids of the 63 kDa gp4A, and consequently lacks primase activity. The primase recognition sites on ssDNA are 5??-G/TGGTC, and 5??-GTGTC. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1849948 1 gene 4.2 range1849948 1 1424 1762 annotation1849950 1 phi4c range1849950 1 1080 1114 annotation1862190 1 gat->gac 4.1 start range1862190 1 70 72 annotation1881252 1 gac...tac -> gat ... tat codon shuffle range1881252 1 4 39 annotation1883672 1 added ngoMIV range1883672 1 1702 1707 annotation1862194 1 codon shuffled (13 changes) phi4c range1862194 1 1078 1116 annotation1862192 1 ttt->TTC S4 -35 range1862192 1 586 588 annotation1849946 1 gene 4B range1849946 1 190 1701 annotation1849949 1 S4: possible promoter range1849949 1 574 637 annotation1862193 1 tta->CUG S4-10 range1862193 1 610 612 annotation1862191 1 cat->CAC 4.2 start range1862191 1 1423 1425 annotation1849942 1 gene 4A range1849942 1 1 1701 annotation1849947 1 gene 4.1 range1849947 1 71 193 BBa_Z0154 1 BBa_Z0154 T7.2 gene 6 2006-04-30T11:00:00Z 2015-05-08T01:14:54Z 3' end mutated to remove gene 6.3 startsite and alter ribosome binding site false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1850751 1 g->A mutation to alter gene 6.3 RBS range1850751 1 882 882 annotation1884018 1 added xhoI range1884018 1 904 909 annotation1850750 1 g->C mutation to remove gene 6.3 start range1850750 1 891 891 BBa_Z0159 1 BBa_Z0159 T7.2 gene 6.5 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z silent mutation at 3' end to remove gene 6.7 RBS. -35 region of F1 probably non-functional at 5' end of gene. false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855744 1 g->C mutation range1855744 1 246 246 annotation1855743 1 g->A mutation range1855743 1 249 249 BBa_Z0138 1 BBa_Z0138 T7.2 gene 4.3 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z conserved; non-essential false false _11_ 0 64 11 Not in stock false false Sriram Kosuri BBa_Z0123 1 BBa_Z0123 T7.2 gene 6.7 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Head protein. ~18 copies per virion. Gp6.7 is not absolutely essential, morphological normal T7 particles are produced in its absence but have reduced infectivity. false false _11_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0109 1 BBa_Z0109 T7 gene 1.3 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation The ATP-dependent DNA ligase is only essential in E. coli lig mutants, but its presence enhances phage growth in wild-type hosts. false false _10_ 0 250 10 Not in stock false false T7.2 annotation1883669 1 added eagI range1883669 1 1081 1086 BBa_Z0252 1 BBa_Z0252 T7 weak binding and processivity 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476345 1 t -> a mutation from consensus range1476345 1 21 21 annotation1476344 1 tga -> acg mutation from consensus range1476344 1 10 12 annotation1476343 1 23bp conserved region range1476343 1 6 28 BBa_Z0127 1 BBa_Z0127 T7.2 gene 10A/B 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z major/minor capsid protein. The 10B portion is non-essential, 10A-only phages seem to grow normally. ~430 copies of 10A and 16 copies of 10B in capsid (415 expected from symmetry). JJ Dunn removed various pieces of terminator.. if you remove hairpin, the gene 10 RNA is obliterated. Also, got rid of gene 10 pre-shine dalgarno and could not get translation off gene 10. (Dunn JJ, PC) false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1850773 1 gene 10A range1850773 1 1 1038 annotation1850774 1 gene 10B range1850774 1 1 1196 annotation1883679 1 added XmaI range1883679 1 1197 1202 BBa_Z0301 1 BBa_Z0301 Venus YFP - E. coli codon optimized 2006-06-20T11:00:00Z 2015-05-08T01:14:55Z Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab. Brighter Variant than standard EYFP. false false _11_ 0 64 10 It's complicated false None really false Sriram Kosuri annotation1881736 1 gtc ...gtt -> gta ... gtt codon shuffle range1881736 1 4 39 BBa_Z0156 1 BBa_Z0156 T7.2 gene 14 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z Mutations on 3' end to remove Gene 15 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855266 1 g->C mutation range1855266 1 582 582 annotation1855267 1 g->C mutation range1855267 1 585 585 annotation1881264 1 tgt...ggc->tgc...ggc codon shuffle range1881264 1 4 39 annotation1884022 1 added SacII range1884022 1 592 597 BBa_Z0152 1 BBa_Z0152 T7.2 gene 2.5 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z mutation from T7 to remove RBS from gene 2.8 false false _10_ 0 64 11 Not in stock false false Sriram Kosuri annotation1881251 1 ggc...gct->ggc..gct codon shuffle range1881251 1 4 39 annotation1860893 1 S2 -35 region range1860893 1 16 20 annotation1860894 1 S2 -10 region range1860894 1 40 45 annotation1860895 1 ttc -> TTT range1860895 1 16 18 annotation1849934 1 S2: possible promoter range1849934 1 3 66 annotation1849919 1 a->G mutation to remove RBS range1849919 1 690 690 BBa_Z0126 1 BBa_Z0126 T7.2 gene 9 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Capsid scaffolding protein false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855246 1 possible promoter site? range1855246 1 522 522 annotation1855247 1 possible promoter site? range1855247 1 671 671 annotation1881254 1 ggc...ggc -> ggc...ggc codon shuffle range1881254 1 4 39 BBa_Z0262 1 BBa_Z0262 Medium strength T7.2 RBS 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri BBa_Z0261 1 BBa_Z0261 Strong T7.2 RBS 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri BBa_Z0134 1 BBa_Z0134 T7.2 gene 17 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z T7 tail fiber protein of phage capsid (adsorption); 14 +/- 3 (eighteen expected by symmettry) copies per virion; 3 copies per tail fiber false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1884038 1 added PvuI range1884038 1 1663 1668 BBa_Z0251 1 BBa_Z0251 T7 strong promoter 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z bacteriophage T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476333 1 consensus sequence range1476333 1 6 28 BBa_Z0136 1 BBa_Z0136 T7.2 gene 18 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z small terminase subunit false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1884021 1 added AGCTT to form HindIII range1884021 1 271 275 BBa_Z0294_sequence 1 caacttaaagagacttaaaagattaatttaaaatttatcaaaaagagtattgacttaaagtctaacctataggatacttacagccatcgagagggacacggcgaatagccatcccaatcgacaccggggtcaaccggataagtagacagcctgataagtcgcacgaaaaacaggtattgacaacatgaagtaacatgcagtaagatacaaatcgctaggtaacactagcagcgtcaaccgggcgcacagtgccttctaggtgacttaagcgcaccacggcacataaggtgaaacaaaacggttgacaacatgaagtaaacacggtacgatgtaccacatgaaacgacagtgagtcaccacactgaaaggtgatgcggtctaacgaaacctgacctaagacgctctttaacaatctggtaaatagctcttgagctc BBa_Z0148_sequence 1 atgttccgcttattgttgaacctactgcggcatagagtcacctaccgatttcttgtggtactttgtgctgcccttgggtacgcatctcttactggagacctcagttcactggagtctgtcgtttgctctatactcacttgtagcgattag BBa_Z0155_sequence 1 atgatgactataagacctactaaaagtacagactttgaggtattcactccggctcaccatgacattcttgaagctaaggctgctggtattgagccgagtttccctgatgcttccgagtgtgtcacgttgagcctctatgggttccctctagctatcggtggtaactgcggggaccagtgctggttcgttacgagcgaccaagtgtggcgacttagtggaaaggctaagcgaaagttccgtaagttaatcatggagtatcgcgataagatgcttgagaagtatgatactctttggaattacgtatgggtaggcaatacgtcccacattcgtttcctcaagactatcggtgcggtattccatgaagagtacacacgagatggtcaatttcagttatttacaatcacgaaaggcggctaa BBa_Z0251_sequence 1 gaaattaatacgactcactatagggagaccacaac BBa_Z0159_sequence 1 atgttaacacctattaaccaattacttaagaaccctaacgatattccagatgtacctcgtgcaaccgctgagtatctacaggttcgattcaactatgcgtacctcgaagcgtctggtcatataggacttatgcgtgctaatggttgtagtgaggcccacatcttgggtttcattcagggcctacagtatgcctctaacgtcattgacgagattgagttacgcaaggaacaactaagagatgatggcgaagattga BBa_Z0106_sequence 1 atgaacattaccgacatcatgaacgctatcgacgcaatcaaagcactgccaatctgtgaacttgacaagcgtcaaggtatgcttatcgacttactggtcgagatggtcaacagcgagacgtgtgatggcgagctaaccgaactaaatcaggcacttgagcatcaagattggtggactaccttgaagtgtctcacggctgacgcagggttcaagatgctcggtaatggtcacttctcggctgcttatagtcacccgctgctacctaacagagtgattaaggtgggctttaagaaagaggattcaggcgcagcctataccgcattctgccgcatgtatcagggtcgtcctggtatccctaacgtctacgatgtacagcgccacgctggatgctatacggtggtacttgacgcacttaaggattgcgagcgtttcaacaatgatgcccattataaatacgctgagattgcaagcgacatcattgattgcaattcggatgagcatgatgagttaactggatgggatggtgagtttgttgaaacttgtaaactaatccgcaagttctttgagggcatcgcctcattcgacatgcatagcgggaacatcatgttctcaaatggagacgtaccatacatcaccgacccggtatcattctcgcagaagaaagacggtggcgcattcagcatcgaccctgaggaactcatcaaggaagtcgaggaagtcgcacgacagaaagaaattgaccgcgctaaggcccgtaaagaacgtcacgaggggcgcttagaggcacgcagattcaaacgtcgcaaccgcaaggcacgtaaagcacacaaagctaagcgcgaaagaatgcttgctgcgtggcgatgggctgaacgtcaagaacggcgtaaccatgaggtagctgtagatgtactaggaagaaccaataacgctatgctctgggtcaacatgttctctggggactttaaggcgcttgaggaacgaatcgcgctgcactggcgtaatgctgaccggatggctatcgctaatggtcttacgctcaacattgataagcaacttgacgcaatgttaatgggctgacgtacg BBa_Z0133_sequence 1 atggataagtacgataagaacgtaccaagtgattatgatggtctgttccaaaaggctgctgatgccaacggggtctcttatgaccttttacgtaaagtcgcttggacagaatcacgatttgtgcctacagcaaaatctaagactggaccattaggcatgatgcaatttaccaaggcaaccgctaaggccctcggtctgcgagttaccgatggtccagacgacgaccgactgaaccctgagttagctattaatgctgccgctaagcaacttgcaggtctggtagggaagtttgatggcgatgaactcaaagctgcccttgcgtacaaccaaggcgagggacgcttgggtaatccacaacttgaggcgtactctaagggagacttcgcatcaatctctgaggagggacgtaactacatgcgtaaccttctggatgttgctaagtcacctatggctggacagttggaaacttttggtggcataaccccaaagggtaaaggcattccggctgaggtaggattggctggaattggtcacaagcagaaagtaacacaggaacttcctgagtccacaagttttgacgttaagggtatcgaacaggaggctacggcgaaaccattcgccaaggacttttgggagacccacggagaaacacttgacgagtacaacagtcgttcaaccttcttcggattcaaaaatgctgccgaagctgaactctccaactcagtcgctgggatggctttccgtgctggtcgtctcgataatggttttgatgtgtttaaagacaccattacgccgactcgctggaactctcacatctggactccagaggagttagagaagattcgaacagaggttaagaaccctgcgtacatcaacgttgtaactggtggttcccctgagaacctcgatgacctcattaaattggctaacgagaactttgagaatgactcccgcgctgccgaggctggcctaggtgccaaactgagtgctggtattattggtgctggtgtggacccgcttagctatgttcctatggtcggtgtcactggtaagggctttaagttaatcaataaggctcttgtagttggtgccgaaagtgctgctctgaacgttgcatccgaaggtctccgtacctccgtagctggtggtgacgcagactatgcgggtgctgccttaggtggctttgtgtttggcgcaggcatgtctgcaatcagtgacgctgtagctgctggactgaaacgcagtaaaccagaagctgagttcgacaatgagttcatcggtcctatgatgcgattggaagcccgtgagacagcacgaaacgccaactctgcggacctctctcggatgaacactgagaacatgaagtttgaaggtgaacataatggtgtcccttatgaggacttaccaacagagagaggtgccgtggtgttacatgatggctccgttctaagtgcaagcaacccaatcaaccctaagactctaaaagagttctccgaggttgaccctgagaaggctgcgcgaggaatcaaactggctgggttcaccgagattggcttgaagaccttggggtctgacgatgctgacatccgtagagtggctatcgacctcgttcgctctcctactggtatgcagtctggtgcctcaggtaagttcggtgcaacagcttctgacatccatgagagacttcatggtactgaccagcgtacttataatgacttgtacaaagcaatgtctgacgctatgaaagaccctgagttctctactggcggcgctaagatgtcccgtgaagaaactcgatacactatctaccgtagagcggcactagctattgagcgtccagaactacagaaggcactcactccgtctgagagaatcgttatggacatcattaagcgtcactttgacaccaagcgtgaacttatggaaaacccagcaatattcggtaacacaaaggctgtgagtatcttccctgagagtcgccacaaaggtacttacgttcctcacgtatatgaccgtcatgccaaggcgctgatgattcaacgctacggtgccgaaggtttgcaggaagggattgcccgctcatggatgaacagctacgtctccagacctgaggtcaaggccagagtcgatgagatgcttaaggaattacacggggtgaaggaagtaacaccagagatggtagagaagtacgctatggataaggcttatggtatctcccactcagaccagttcaccaacagttccataatagaagagaacattgagggcttagtaggtatcgagaataactcattccttgaggcacgtaacttgtttgattcggacctatccatcactatgccagacggacagcaattctcagtgaatgacctaagggacttcgatatgttccgcatcatgccagcgtatgaccgccgtgtcaatggtgacatcgccatcatggggtctactggtaaaaccactaaggaacttaaggatgagattttggctctcaaagcgaaagctgagggagacggtaagaagactggcgaggtacatgctttaatggataccgttaagattcttactggtcgtgctagacgcaatcaggacactgtgtgggaaacctcactgcgtgccatcaatgacctagggttcttcgctaagaacgcctacatgggtgctcagaacattacggagattgctgggatgattgtcactggtaacgttcgtgctctagggcatggtatcccaattctgcgtgatacactctacaagtctaaaccagtttcagctaaggaactcaaggaactccatgcgtctctgttcgggaaggaggtggaccagttgattcggcctaaacgtgctgacattgtgcagcgcctaagggaagcaactgataccggacctgccgtggcgaacatcgtagggaccttgaagtattcaacacaggaactggctgctcgctctccgtggactaagctactgaacggaaccactaactaccttctggatgctgcgcgtcaaggtatgcttggggatgttattagtgccaccctaacaggtaagactacccgctgggagaaagaaggcttccttcgtggtgcctccgtaactcctgagcagatggctggcatcaagtctctcatcaaggaacatatggtacgcggtgaggacgggaagtttaccgttaaggacaagcaagcgttctctatggacccacgggctatggacttatggagactggctgacaaggtagctgatgaggcaatgctgcgtccacataaggtgtccttacaggattcccatgcgttcggagcactaggtaagatggttatgcagtttaagtctttcactatcaagtcccttaactctaagttcctgcgaaccttctatgatggatacaagaacaaccgagcgattgacgctgcgctgagcatcatcacctctatgggtctcgctggtggtttctatgctatggctgcacacgtcaaagcatacgctctgcctaaggagaaacgtaaggagtacttggagcgtgcactggacccaaccatgattgcccacgctgcgttatctcgtagttctcaattgggtgctcctttggctatggttgacctagttggtggtgttttagggttcgagtcctccaagatggctcgctctacgattctacctaaggacaccgtgaaggaacgtgacccaaacaaaccgtacacctctagagaggtaatgggcgctatgggttcaaaccttctggaacagatgccttcggctggctttgtggctaacgtaggggctaccttaatgaatgctgctggcgtggtcaactcacctaataaagcaaccgagcaggacttcatgactggtcttatgaactccacaaaagagttagtaccgaacgacccactgacccaacagcttgtgttgaaaatctacgaggcgaacggtgttaacttgagggagcgtaggaaataa BBa_Z0272_sequence 1 taactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctg BBa_Z0127_sequence 1 atggctagcatgactggtggacagcaaatgggtactaaccaaggtaaaggtgtagttgctgctggagataaactggcgttgttcttgaaggtatttggcggtgaagtcctgactgcgttcgctcgtacctccgtgaccacttctcgccacatggtacgttccatctccagcggtaaatccgctcagttccctgttctgggtcgcactcaggcagcgtatctggctccgggcgagaacctcgacgataaacgtaaggacatcaaacacaccgagaaggtaatcaccattgacggtctcctgacggctgacgttctgatttatgatattgaggacgcgatgaaccactacgacgttcgctctgagtatacctctcagttgggtgaatctctggcgatggctgcggatggtgcggttctggctgagattgccggtctgtgtaacgtggaaagcaaatataatgagaacatcgagggcttaggtactgctaccgtaattgagaccactcagaacaaggccgcacttaccgaccaagttgcgctgggtaaggagattattgcggctctgactaaggctcgtgcggctctgaccaagaactatgttccggctgctgaccgtgtgttctactgtgacccagatagctactctgcgattctggcagcactgatgccgaacgcagcaaactacgctgctctgattgaccctgagaagggttctatccgcaacgttatgggctttgaggttgtagaagttccgcacctcaccgctggtggtgctggtaccgctcgtgagggcactactggtcagaagcacgtcttccctgccaataaaggtgagggtaatgtcaaggttgctaaggacaacgttatcggcctgttcatgcaccgctctgcggtaggtactgttaagctgcgtgacttggctctggagcgcgctcgccgtgctaacttccaagcggaccagattatcgctaagtacgcaatgggccacggtggtcttcgcccagaagctgctggtgcagtggttttcaaagtggagtaatgctgggggtggcctcaacggtcgctgctagtcccgaagaggcgagtgttacttcaacagaagaaaccttaacgccagcacaggaggccgcacgcacccgcgctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataacccggg BBa_Z0293_sequence 1 ggtcttcctgaccgactgatggctcaccgagggattcagcggtatgattgcatcacaccacttcatccctatagagtcaagtcctaaggtatacccataaagagcctctaatggtctatcctaaggtctatacctaaagataggccatcctatcagtgtcacctaaagagggtcttagagagggcctatggagttcctatagggtcctttaaaatataccataaaaatctgagtgactatctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccct BBa_Z0126_sequence 1 atggcagagagtaacgcagatgtatacgcaagttttggcgtgaactccgctgtgatgtctggtggttccgttgaggaacatgagcagaacatgctggctcttgatgttgctgcccgtgatggcgatgatgcaatcgagttagcgtcagacgaagtggaaacagaacgtgacctgtatgacaactctgacccgttcggtcaagaggatgacgaaggccgcattcaggttcgtatcggtgatggctctgagccgaccgatgtggacactggagaagaaggcgttgagggcaccgaaggttccgaagagtttaccccactgggcgagactccagaagaactggtagctgcctctgagcaacttggtgagcacgaagagggcttccaagagatgattaacattgctgctgagcgtggcatgagtgtcgagaccattgaggctatccagcgtgagtacgaggagaacgaagagttgtccgccgagtcctacgctaagctggctgaaattggctacacgaaggctttcattgactcgtatatccgtggtcaagaagctctggtggagcagtacgtaaacagtgtcattgagtacgctggtggtcgtgaacgttttgatgcactgtataaccaccttgagacgcacaaccctgaggctgcacagtcgctggataatgcgttgaccaatcgtgacttagcgaccgttaaggctatcatcaacttggctggtgagtctcgcgctaaggcgttcggtcgtaagccaactcgtagtgtgactaatcgtgctattccggctaaacctcaggctaccaagcgtgaaggctttgcggaccgtagcgagatgattaaagctatgagtgaccctcggtatcgcacagatgccaactatcgtcgtcaagtcgaacagaaagtaatcgattcgaacttctga BBa_Z0158_sequence 1 atggctatgtctaacatgacttacaacaacgttttcgaccacgcttacgaaatgctgaaagaaaacatccgttatgatgacatccgtgacactgatgacctgcacgatgctattcacatggctgccgataatgcagttccgcactactacgctgacatctttagcgtaatggcaagtgagggcattgaccttgagttcgaagactctggtctgatgcctgacaccaaggacgtaatccgcatcctgcaagcgcgtatctatgagcaattaacgattgacctctgggaagacgcagaagacttgctcaatgaatacttggaggaagtcgaggagtacgaggaggacgaagagtaagggccc BBa_Z0144_sequence 1 atgctagagttcctccgtaagctaatcccttgggttctcgctgggatgctattcgggttaggatggcatctagggtcagactcaatggacgctaaatggaaacaggaggtacacaatgagtacgttaagagagttgaggctgcgaagagcactcaaagagcaatcgatgcggtatctgctaagtatcaagaagaccttgccgcgctggaagggagcactgataggattatttctgatttgcgtagcgacaataagcggttgcgcgtcagagtcaaaactaccggaacctccgatggtcagtgtggattcgagcctgatggtcgagccgaacttgacgaccgagatgctaaacgtattctcgcagtgacccagaagggtgacgcatggattcgtgcgttacaggatactattcgtgaactgcaacgtaagtagcatgc BBa_Z0156_sequence 1 atgtgctgggcagcagctataccgattgcaataagtggcgctcaggctatcagtggtcagaacgctcaggccaaaatgattgccgctcagaccgctgctggtcgtcgtcaagctatggaaatcatgaggcagacgaacatccagaatgctgacctatcgttgcaagctcgaagtaaacttgaggaagcgtccgccgagttgacctcacagaacatgcagaaggtccaagctattgggtctatccgagcggctatcggagagagtatgcttgaaggttcctcaatggaccgcattaagcgagtcacagaaggacagttcattcgggaagccaatatggtaactgagaactatcgccgtgactaccaagcaatcttcgcacagcaacttggtggtactcaaagtgctgcaagtcagattgacgaaatctataagagcgaacagaaacagaagagtaagctacagatggttctggacccactggctatcatggggtcttccgctgcgagtgcttacgcatccggtgcgttcgactctaagtccacaactaaggcacctattgttgccgctaaaggaaccaagaccggcaggtaaccgcgg BBa_Z0128_sequence 1 atgcgaagttatgatatgaatgtagaaacagcggcagaattatcagctgtgaacgacattctggcgtctatcggtgaacctccggtatcaacgctggaaggtgacgctaacgcagatgcagcgaacgctcggcgtattctcaacaagattaaccgacagattcaatctcgtggatggacgttcaacattgaggaaggcataacgctactacctgatgtttactccaacctgattgtatacagtgacgactatttatccctaatgtctacttccggtcaatccatctacgttaaccgaggtggctatgtgtatgaccgaacgagtcaatcagaccgctttgactctggtattactgtgaacattattcgtctccgcgactacgatgagatgcctgagtgcttccgttactggattgtcaccaaggcttcccgtcagttcaacaaccgattctttggggcaccggaagtagagggtgtactccaagaagaggaagatgaggctagacgtctctgcatggagtatgagatggactacggtgggtacaatatgctggatggagatgcgttcacttctggtctactgactcgctaa BBa_Z0252_sequence 1 ataattaattgaactcactaaagggagaccacagc BBa_Z0503_sequence 1 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gtaggtatcactggtcagttaactggtagccgtgctgacattatcattgcggatgacgttgagattccgtctaacagcgcaactatgggtgcccgtgagaagctatggactctggttcaggagttcgctgcgttacttaaaccgctgccttcctctcgcgttatctaccttggtacacctcagacagagatgactctctataaggaacttgaggataaccgtgggtacacaaccattatctggcctgctctgtacccaaggacacgtgaagagaacctctattactcacagcgtcttgctcctatgttacgcgctgagtacgatgagaaccctgaggcacttgctgggactccaacagacccagtgcgctttgaccgtgatgacctgcgcgagcgtgagttggaatacggtaaggctggctttacgctacagttcatgcttaaccctaaccttagtgatgccgagaagtacccgctgaggcttcgtgacgctatcgtagcggccttagacttagagaaggccccaatgcattaccagtggcttccgaaccgtcagaacatcattgaggaccttcctaacgttggccttaagggtgatgacctgcatacgtaccacgattgttccaacaactcaggtcagtaccaacagaagattctggtcattgaccctagtggtcgcggtaaggacgaaacaggttacgctgtgctgtacacactgaacggttacatctaccttatggaagctggaggtttccgtgatggctactccgataagacccttgagttactcgctaagaaggcaaagcaatggggagtccagacggttgtctacgagagtaacttcggtgacggtatgttcggtaaggtattcagtcctatccttcttaaacaccacaactgtgcgatggaagagattcgtgcccgtggtatgaaagagatgcgtatttgcgatacccttgagccagtcatgcagactcaccgccttgtaattcgtgatgaggtcattagggccgactaccagtccgctcgtgacgtagacggtaagcatgacgttaagtactcgttgttctaccagatgacccgtatcactcgtgagaaaggcgctctggctcatgatgaccgattggatgcccttgcgttaggcattgagtatctccgtgagtccatgcagttggattccgttaaggtcgagggtgaagtacttgctgacttccttgaggaacacatgatgcgtcctacggttgctgctacgcatatcattgagatgtctgtgggaggagttgatgtgtactctgaggacgatgagggttacggtacgtctttcattgagtggtgagaaattaatacgactcactatagggagaccacaacaatcaataggagaaatcaatatgttccgcttattgttgaacctactgcggcatagagtcacctaccgatttcttgtggtactttgtgctgcccttgggtacgcatctcttactggagacctcagttcactggagtctgtcgtttgctctatactcacttgtagcgattagggtcttcctgaccgactgatggctcaccgagggattcagcggtatgattgcatcacaccacttcatccctatagagtcaagtcctaaggtatacccataaagagcctctaatggtctatcctaaggtctatacctaaagataggccatcctatcagtgtcacctaaagagggtcttagagagggcctatggagttcctatagggtcctttaaaatataccataaaaatctgagtgactatctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccct BBa_Z0108_sequence 1 atgcgtaacttcgaaaagatgaccaaacgttctaaccgtaatgctcgtgacttcgaggcaaccaaaggtcgcaagttgaataagactaagcgtgaccgctctcacaagcgtagctgggagggtcagtaa BBa_Z0132_sequence 1 atgagtaaaattgaatctgcccttcaagcggcacaaccgggactctctcggttacgtggtggtgctggaggtatgggctatcgtgcagcaaccactcaggccgaacagccaaggtcaagcctattggacaccattggtcggttcgctaaggctggtgccgatatgtataccgctaaggaacaacgagcacgagacctagctgatgaacgctctaacgagattatccgtaagctgacccctgagcaacgtcgagaagctctcaacaacgggacccttctgtatcaggatgacccatacgctatggaagcactccgagtcaagactggtcgtaacgctgcgtatcttgtggacgatgacgttatgcagaagataaaagagggtgtcttccgtactcgcgaagagatggaagagtatcgccatagtcgccttcaagagggcgctaaggtatacgctgagcagttcggcatcgaccctgaggacgttgattatcagcgtggtttcaacggggacattaccgagcgtaacatctcgctgtatggtgcgcatgataacttcttgagccagcaagctcagaagggcgctatcatgaacagccgagtggaactcaacggtgtccttcaagaccctgatatgctgcgtcgtccagactctgctgacttctttgagaagtatatcgacaacggtctggttactggcgcaatcccatctgatgctcaagccacacagcttataagccaagcgttcagtgacgcttctagccgtgctggtggtgctgacttcctgatgcgagtcggtgacaagaaggtaacacttaacggagccactacgacttaccgagagttgattggtgaggaacagtggaacgctctcatggtcacagcacaacgttctcagtttgagactgacgcgaagctgaacgagcagtatcgcttgaagattaactctgcgctgaaccaagaggacccaaggacagcttgggagatgcttcaaggtatcaaggctgaactagataaggtccaacctgatgagcagatgacaccacaacgtgagtggctaatctccgcacaggaacaagttcagaatcagatgaacgcatggacgaaagctcaggccaaggctctggacgattccatgaagtcaatgaacaaacttgacgtaatcgacaagcaattccagaagcgaatcaacggtgagtgggtctcaacggattttaaggatatgccagtcaacgagaacactggtgagttcaagcatagcgatatggttaactacgccaataagaagctcgctgagattgacagtatggacattccagacggtgccaaggatgctatgaagttgaagtaccttcaagcggactctaaggacggagcattccgtacagccatcggaaccatggtcactgacgctggtcaagagtggtctgccgctgtgattaacggtaagttaccagaacgaaccccagctatggatgctctgcgcagaatccgcaatgctgaccctcagttgattgctgcgctatacccagaccaagctgagctattcctgacgatggacatgatggacaagcagggtattgaccctcaggttattcttgatgccgaccgactgactgttaagcggtccaaagagcaacgctttgaggatgataaagcattcgagtctgcactgaatgcatctaaggctcctgagattgcccgtatgccagcgtcactgcgcgaatctgcacgtaagatttatgactccgttaagtatcgctcggggaacgaaagcatggctatggagcagatgaccaagttccttaaggaatctacctacacgttcactggtgatgatgttgacggtgataccgttggtgtgattcctaagaatatgatgcaggttaactctgacccgaaatcatgggagcaaggtcgggatattctggaggaagcacgtaagggaatcattgcgagcaacccttggataaccaataagcaactgaccatgtattctcaaggtgactccatttaccttatggacaccacaggtcaagtcagagtccgatacgacaaagagttactctcgaaggtctggagtgagaaccagaagaaactcgaagagaaagctcgtgagaaggctctggctgatgtgaacaagcgagcacctatagttgccgctacgaaggcccgtgaagctgctgctaaacgagtccgagagaaacgtaaacagactcctaagttcatctacggacgtaaggagtaa BBa_Z0160_sequence 1 atggctatgacaaagaaatttaaagtgtccttcgacgttaccgcaaagatgtcgtctgacgttcaggcaatcttagagaaagatatgctgcatctatgtaagcaggtcggctcaggtgcgattgtccccaatggtaaacagaaggaaatgattgtccagttcctgacacacggtatggaaggattgatgacattcgtagtacgtacatcatttcgtgaggccattaaggacatgcacgaagagtatgcagataaggactctttcaaacaatctcctgcaacagtacgggaggtgttctgatgtctgactacctgaaagtgctgcaagcaatcaaaagttgccctaagactttccagtccaactatgtacggaacaatgcgagcctcgtagcggaggccgcttcccgtggtcacatctcgtgcctgactactagtggacgtaacggtggcgcttgggaaatcactgcttccggtactcgctttctgaaacgaatgggcgggtgtgtctaa BBa_Z0116_sequence 1 atgagcaatgtaaataccggatccttaagcgtagataataagaagttttgggctaccgtagagtcctcggagcattccttcgaggttccaatctacgctgagaccctagacgaagctctggagttagccgaatggcaatacgttccggctggctttgaggttactcgtgtgcgtccttgtgtagcaccgaagtaagaattc BBa_Z0273_sequence 1 gtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcac BBa_Z0118_sequence 1 atggcaggttacggcgctaaaggaatccgaaaggttggagcgtttcgctctggcctagaggacaaggtttcaaagcagttggaatcaaaaggtattaaattcgagtatgaagagtggaaagtgccttatgtaattccggcgagcaatcacacttacactccagacttcttacttccaaacggtatattcgttgagacaaagggtctgtgggaaagcgatgatagaaagaagcacttattaattagggagcagcaccccgagctagacatccgtattgtcttctcaagctcacgtactaagttatacaaaggttctccaacgtcttatggagagttctgcgaaaagcatggtattaagttcgctgataaactgatacctgctgagtggataaaggaacccaagaaggaggtcccctttgatagattaaaaaggaaaggaggaaagaaataa BBa_Z0261_sequence 1 aatcaataggagaaatcaat BBa_Z0152_sequence 1 atggcgaaaaaaatatttacaagtgcgttaggaacagctgaaccttacgcttacatcgccaagccggactacggcaacgaagagcgtggctttgggaaccctcgtggtgtctataaagttgacctgactattcccaacaaagacccgcgctgccagcgtatggtcgatgaaatcgtgaagtgtcacgaagaggcttatgctgctgccgttgaggaatacgaagctaatccacctgctgtagctcgtggtaagaaaccgctgaaaccgtatgagggtgacatgccgttcttcgataacggtgacggtacgactacctttaagttcaaatgctacgcgtctttccaagacaagaagaccaaagagaccaagcacatcaatctggttgtggttgactcaaaaggtaagaagatggaagacgttccgattatcggtggtggctctaagctgaaagttaaatattctctggttccatacaagtggaacactgctgtaggtgcgagcgttaagctgcaactggaatccgtgatgctggtcgaactggctacctttggtggcggtgaagacgattgggctgacgaagttgaagagaacggctatgttgcctctggttctgccaaagcgagcaaaccacgcgacgaagaaagctgggacgaagacgacgaagagtccgaggaagcagacgaagacggggacttctaa BBa_Z0137_sequence 1 gtgagcacacagtccaatcgaaacgcattagttgtagctcaactgaaaggagacttcgtggcgttcctattcgtcttatggaaggcgctaaacctaccggtgcccactaagtgtcagattgacatggctaaggtgctggcgaatggagacaacaagaagttcatcttacaggctttccgtggtatcggtaagtcgttcatcacatgtgcgttcgttgtgtggtccttatggagagaccctcagttgaagatacttatcgtatcagcctctaaggagcgtgcagacgctaactccatctttattaagaacatcattgacctgctgccattcctatctgagttaaagccaagacccggacagcgtgactcggtaatcagctttgatgtaggcccagccaatcctgaccactctcctagtgtgaaatcagtaggtatcactggtcagttaactggtagccgtgctgacattatcattgcggatgacgttgagattccgtctaacagcgcaactatgggtgcccgtgagaagctatggactctggttcaggagttcgctgcgttacttaaaccgctgccttcctctcgcgttatctaccttggtacacctcagacagagatgactctctataaggaacttgaggataaccgtgggtacacaaccattatctggcctgctctgtacccaaggacacgtgaagagaacctctattactcacagcgtcttgctcctatgttacgcgctgagtacgatgagaaccctgaggcacttgctgggactccaacagacccagtgcgctttgaccgtgatgacctgcgcgagcgtgagttggaatacggtaaggctggctttacgctacagttcatgcttaaccctaaccttagtgatgccgagaagtacccgctgaggcttcgtgacgctatcgtagcggccttagacttagagaaggccccaatgcattaccagtggcttccgaaccgtcagaacatcattgaggaccttcctaacgttggccttaagggtgatgacctgcatacgtaccacgattgttccaacaactcaggtcagtaccaacagaagattctggtcattgaccctagtggtcgcggtaaggacgaaacaggttacgctgtgctgtacacactgaacggttacatctaccttatggaagctggaggtttccgtgatggctactccgataagacccttgagttactcgctaagaaggcaaagcaatggggagtccagacggttgtctacgagagtaacttcggtgacggtatgttcggtaaggtattcagtcctatccttcttaaacaccacaactgtgcgatggaagagattcgtgcccgtggtatgaaagagatgcgtatttgcgatacccttgagccagtcatgcagactcaccgccttgtaattcgtgatgaggtcattagggccgactaccagtccgctcgtgacgtagacggtaagcatgacgttaagtactcgttgttctaccagatgacccgtatcactcgtgagaaaggcgctctggctcatgatgaccgattggatgcccttgcgttaggcattgagtatctccgtgagtccatgcagttggattccgttaaggtcgagggtgaagtacttgctgacttccttgaggaacacatgatgcgtcctacggttgctgctacgcatatcattgagatgtctgtgggaggagttgatgtgtactctgaggacgatgagggttacggtacgtctttcattgagtggtga BBa_Z0120_sequence 1 atggataatagtcatgatagcgatagcgtttttttgtatcacattccttgtgacaactgtgggagtagtgacgggaactcgctgttctctgacggacacacgttctgctacgtatgcgagaagtggactgctggtaatgaagacactaaagagagggcttcaaaacggaaaccctcaggaggtaaaccaatgacttacaacgtgtggaacttcggggaatccaatggacgctactccgcgttaactgcgagaggaatctccaaggaaacctgtcagaaggctggctactggattgccaaagtagacggtgtgatgtaccaagtggctgactatcgggaccagaacggcaacattgtgagtcagaaggttcgagataaagataagaactttaagaccactggtagtcacaagagtgacgctctgttcgggaagcacttgtggaatggtggtaagaagattgtcgttacagaaggtgaaatcgacatgcttaccgtgatggaacttcaagactgtaagtatcctgtagtgtcgttgggtcacggtgcctctgccgctaagaagacatgcgctgccaactacgaatacttcgatcagttcgaacagattatcctgatgttcgatatggacgaagcagggcgcaaagcagtcgaagaggctgcacaggttctacctgctggtaaggtacgagtggcagttcttccgtgtaaggatgcaaacgagtgtcacctaaatggtcacgaccgtgaaatcatggagcaagtgtggaatgctggtccttggattcctgatggtgtggtatcggctctttcgttacgtgaacgaatccgtgagcacctatcgtccgaggaatcagtaggtttacttttcagtggctgcactggtatcaacgataagaccttaggtgcccgtggtggtgaagtcattatggtcacttccggttccggtatgggtaagtcaacgttcgtccgtcaacaagctctacaatggggcacagcgatgggcaagaaggtaggcttagcgatgcttgaggagtccgttgaggagaccgctgaggaccttataggtctacacaaccgtgttcgtctgcgtcagtctgactctctgaaacgtgaaatcattgagaacggtaagttcgaccaatggttcgatgaactgttcggcaacgatacgttccatctatatgactcattcgccgaggctgagacggatagactgctcgctaagctggcctacatgcgctcaggcttgggctgtgacgtaatcattctagaccacatctcaatcgtcgtatccgcttctggtgaatccgatgagcgtaagatgattgacaacctgatgaccaagctcaaagggttcgctaagtcaactggggtggtgctggtcgtaatttgtcaccttaagaacccagacaaaggtaaagcacacgaggaaggtcgccccgtttctattactgacctacgtggttctggcgcactacgccaactatctgatactattattgcccttgagcgtaatcagcaaggcgatatgcctaaccttgtcctcgttcgtattctcaagtgccgctttactggtgatactggtatcgctggctacatggaatacaacaaggaaaccggatggcttgaaccatcaagttactcaggggaagaagagtcacactcagagtcaacagactggtccaacgacactgacttctaagccggc BBa_Z0154_sequence 1 atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggaaatttacctctggagaccgtagctcgag BBa_Z0138_sequence 1 atgttcaaactgattaagaagttaggccaactgctggttcgtatgtacaacgtggaagccaagcgactgaacgatgaggctcgtaaagaggccacacagtcacgcgctctggcgattcgctccaacgaactggctgacagtgcatccactaaagttaccgaggctgcccgtgtggcaaaccaagctcaacagctttccaaattctttgagtaa BBa_Z0124_sequence 1 atgggtaagaaagttaagaaggccgtgaagaaagtcaccaagtccgttaagaaagtcgttaaggaaggggctcgtccggttaaacaggttgctggcggtctagctggtctggctggtggtactggtgaagcacagatggtggaagtaccacaagctgccgcacagattgttgacgtacctgagaaagaggtttccactgaggacgaagcacagacagaaagcggacgcaagaaagctcgtgctggcggtaagaaatccttgagtgtagcccgtagctccggtggcggtatcaacatctaaggtcacc BBa_Z0139_sequence 1 atgtctaacgtagctgaaactatccgtctatccgatacagctgaccagtggaaccgtcgagtccacatcaacgttcgcaacggtaaggcgactatggtttaccgctggaaggactctaagtcctctaagaatcacactcagcgtatgacgttgacagatgagcaagcactgcgtctggtcaatgcgcttaccaaagctgccgtgacagcaattcatgaagctggtcgcgtcaatgaagctatggctatcctcgacaagattgataactaa BBa_Z0301_sequence 1 atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa BBa_Z0115_sequence 1 atgggacgtttatatagtggtaatctggcagcattcaaggcagcaacaaacaagctgttccagttagacttagcggtcatttatgatgactggtatgatgcctatacaagaaaagattgcatacggttacgtattgaggacaggagtggaaacctgattgatactagcaccttctaccaccacgacgaggacgttctgttcaatatgtgtactgattggttgaaccatatgtatgaccagttgaaggactggaagtaa BBa_Z0271_sequence 1 cacactggctcaccttcgggtgggcctttctgcgttt BBa_Z0151_sequence 1 atgggactgttagatggtgaagcctgggaaaaagaaaacccgccagtacaagcaactgggtgtatagcttgcttagagaaagatgaccgttatccacacacctgtaacaaaggagctaacgatatgaccgaacgtgaacaagagatgatcattaagttgatagacaataatgaaggtcgcccagatgatttgaatggctgcggtattctctgctccaatgtcccttgccacctctgccccgcaaataacgatcaaaagataaccttaggtgaaatccgagcgatggacccacgtaaaccacatctgaataaacctgaggtaactcctacagatgaccagccttccgctgagacaatcgaaggtgtcactaagccttcccactacatgctgtttgacgacattgaggctatcgaagtgattgctcgttcaatgaccgttgagcagttcaagggatactgcttcggtaacatcttaaagtacagactacgtgctggtaagaagtcagagttagcgtacttagagaaagacctagcgaaagcagacttctataaagaactctttgagaaacataaagataaatgttacgcataa BBa_Z0107_sequence 1 atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggtagatacatatgagtcttgtgacgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaatccgga BBa_Z0291_sequence 1 taacgccaaatcaatacgactcactatagagggacaaactcaaggtcattcgcaagagtggcctttatgattgaccttcttccggttaatacgactcactataggagaaccttaaggtttaactttaagacccttaagtgttaattagagatttaaatta BBa_Z0262_sequence 1 ttaaagaggagaaatactag BBa_Z0292_sequence 1 tctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccctatctgttacagtctcctaaagtatcctcctaaagtcacctcctaacgtccatcctaaagccaacacctaaagcctacacctaaagacccatcaagtcaacgcctatcttaaagtttaaacataaagaccagacctaaagaccagacctaaagacactacataaagaccagacctaaagacgccttgttgttagccataaagtgataacctttaatca BBa_Z0125_sequence 1 atggctgagaaacgaacaggacttgcggaggatggcgcaaagtctgtctatgagcgtttaaagaacgaccgtgctccctatgagacacgcgctcagaattgcgctcaatataccatcccatcattgttccctaaggactccgataacgcctctacagattatcaaactccgtggcaagccgtgggcgctcgtggtctgaacaatctagcctctaagctcatgctggctctattccctatgcagacttggatgcgacttactatatctgaatatgaagcaaagcagttactgagcgaccccgatggactcgctaaggtcgatgagggcctctcgatggtagagcgtatcatcatgaactacattgagtctaacagttaccgcgtgactctctttgaggctctcaaacagttagtcgtagctggtaacgtcctgctgtacctaccggaaccggaagggtcaaactataatcccatgaagctgtaccgattgtcttcttatgtggtccaacgagacgcattcggcaacgttctgcaaatggtgactcgtgaccagatagcttttggtgctctccctgaggacatccgtaaggctgtagaaggtcaaggtggtgagaagaaagctgatgagacaatcgacgtgtacactcacatctatctggatgaggactcaggtgaatacctccgatacgaagaggtcgagggtatggaagtccaaggctccgatgggacttatcctaaagaggcttgcccatacatcccgattcggatggtcagactagatggtgaatcctacggtcgttcgtacattgaggaatacttaggtgacttacggtcccttgaaaatctccaagaggctatcgtcaagatgtccatgattagctctaaggttatcggcttagtgaatcctgctggtatcacccagccacgccgactgaccaaagctcagactggtgacttcgttactggtcgtccagaagacatctcgttcctccaactggagaagcaagcagactttactgtagctaaagccgtaagtgacgctatcgaggctcgcctttcgtttgcctttatgttgaactctgcggttcagcgtacaggtgaacgtgtgaccgccgaagagattcggtatgtagcttctgaacttgaagatactttaggtggtgtctactctatcctttctcaagaattacaattgcctctggtacgagtgctcttgaagcaactacaagccacgcaacagattcctgagttacctaaggaagccgtagagccaaccattagtacaggtctggaagcaattggtcgaggacaagaccttgataagctggagcggtgtgtcactgcgtgggctgcactggcacctatgcgggacgaccctgatattaaccttgcgatgattaagttacgtattgccaacgctatcggtattgacacttctggtattctactcaccgaagaacagaagcaacagaagatggcccaacagtctatgcaaatgggtatggataatggtgctgctgcgctggctcaaggtatggctgcacaagctacagcttcacctgaggctatggctgctgccgctgattccgtaggtttacagccgggaatttaagtcgac BBa_Z0123_sequence 1 atgtgtttctcaccgaaaattaaaactccgaagatggataccaatcagattcgagccgttgagccagcgcctctgacccaagaagtgtcaagcgtggagttcggtgggtcttctgatgagacggataccgagggcaccgaagtgtctggacgcaaaggcctcaaggtcgaacgtgatgattccgtagcgaagtctaaagccagcggcaatggctccgctcgtatgaaatcttccatccgtaagtccgcatttggaggtaagaagtga BBa_Z0136_sequence 1 atggaaaaggataagagccttattacattcttagagatgttggacactgcgatggctcagcgtatgcttgcggacctttcggaccatgagcgtcgctctccgcaactctataatgctattaacaaactgttagaccgccacaagttccagattggtaagttgcagccggatgttcacatcttaggtggccttgctggtgctcttgaagagtacaaagagaaagtcggtgataacggtcttacggatgatgatatttacacattacagtgaagctt BBa_Z0129_sequence 1 atggcactgatatcccaaagcattaaaaatttaaaaggcggtatcagccaacagcctgacatccttcgttatccagaccaagggtcacgccaagttaacggttggtcttcggagaccgagggcctccaaaagcgtccacctcttgttttcttaaatacacttggagacaacggtgcgttaggtcaagctccgtacatccacctgattaaccgagatgagcacgaacagtattacgctgtgttcactggtagcggaatccgagtgttcgacctttctggtaacgagaagcaagttaggtatcctaacggttccaactacatcaagaccgctaatccacgtaacgacctgcgaatggttactgtagcagactatacgttcatcgtgaaccgtaacgttgttgcacagaagaacacaaagtctgtcaacttaccgaattacaaccctaatcaagacggattgattaacgttcgtggtggtcagtatggtagggaactaattgtacacattaacggtaaagacgttgcgaagtataagataccagatggtagtcaacctgaacacgtaaacaatacggatgcccaatggttagctgaagagttagccaagcagatgcgcactaacttgtctgattggactgtaaatgtagggcaagggttcatccatgtgaccgcacctagtggtcaacagattgactccttcacgactaaagatggctacgcagaccagttgattaaccctgtgacccactacgctcagtcgttctctaagctgccacctaatgctcctaacggctacatggtgaaaatcgtaggggacgcctctaagtctgccgaccagtattacgttcggtatgacgctgagcggaaagtttggactgagactttaggttggaacactgaggaccaagttctatgggaaaccatgccacacgctcttgtgcgagccgctgacggtaatttcgacttcaagtggcttgagtggtctcctaagtcttgtggtgacgttgacaccaacccttggccttcttttgttggttcaagtattaacgatgtgttcttcttccgtaaccgcttaggattccttagtggggagaacatcatattgagtcgtacagccaaatacttcaacttctaccctgcgtccattgcgaaccttagtgatgacgaccctatagacgtagctgtgagtaccaaccgaatagcaatccttaagtacgccgttccgttctcagaagagttactcatctggtccgatgaagcacaattcgtcctgactgcctcgggtactctcacatctaagtcggttgagttgaacctaacgacccagtttgacgtacaggaccgagcgagaccttttgggattgggcgtaatgtctactttgctagtccgaggtccagcttcacgtccatccacaggtactacgctgtgcaggatgtcagttccgttaagaatgctgaggacattacatcacacgttcctaactacatccctaatggtgtgttcagtatttgcggaagtggtacggaaaacttctgttcggtactatctcacggggaccctagtaaaatcttcatgtacaaattcctgtacctgaacgaagagttaaggcaacagtcgtggtctcattgggactttggggaaaacgtacaggttctagcttgtcagagtatcagctcagatatgtatgtgattcttcgcaatgagttcaatacgttcctagctagaatctctttcactaagaacgccattgacttacagggagaaccctatcgtgcctttatggacatgaagattcgatacacgattcctagtggaacatacaacgatgacacattcactacctctattcatattccaacaatttatggtgcaaacttcgggaggggcaaaatcactgtattggagcctgatggtaagataaccgtgtttgagcaacctacggctgggtggaatagcgacccttggctgagactcagcggtaacttggagggacgcatggtgtacattgggttcaacattaacttcgtatatgagttctctaagttcctcatcaagcagactgccgacgacgggtctacctccacggaagacattgggcgcttacagttacgccgagcgtgggttaactacgagaactctggtacgtttgacatttatgttgagaaccaatcgtctaactggaagtacacaatggctggtgcccgattaggctctaacactctgagggctgggagactgaacttagggaccggacaatatcgattccctgtggttggtaacgccaagttcaacactgtatacatcttgtcagatgagactacccctctgaacatcattgggtgtggctgggaaggtaactacttacggagaagttccggtatttaattaa BBa_Z0134_sequence 1 atggctaacgtaattaaaaccgttttgacttaccagttagatggctccaatcgtgattttaatatcccgtttgagtatctagcccgtaagttcgtagtggtaactcttattggtgtagaccgaaaggtccttacgattaatacagactatcgctttgctacacgtactactatctctctgacaaaggcttggggtccagccgatggctacacgaccatcgagttacgtcgagtaacctccactaccgaccgattggttgactttacggatggttcaatcctccgcgcgtatgaccttaacgtcgctcagattcaaacgatgcacgtagcggaagaggcccgtgacctcactacggatactatcggtgtcaataacgatggtcacttggatgctcgtggtcgtcgaattgtgaacctagcgaacgccgtggatgaccgcgatgctgttccgtttggtcaactaaagaccatgaaccagaactcatggcaagcacgtaatgaagccttacagttccgtaatgaggctgagactttcagaaaccaagcggagggctttaagaacgagtccagtaccaacgctacgaacacaaagcagtggcgcgatgagaccaagggtttccgagacgaagccaagcggttcaagaatacggctggtcaatacgctacatctgctgggaactctgcttccgctgcgcatcaatctgaggtaaacgctgagaactctgccacagcatccgctaactctgctcatttggcagaacagcaagcagaccgtgcggaacgtgaggcagacaagctggaaaattacaatggattggctggtgcaattgataaggtagatggaaccaatgtgtactggaaaggaaatattcacgctaacgggcgcctttacatgaccacaaacggttttgactgtggccagtatcaacagttctttggtggtgtcactaatcgttactctgtcatggagtggggagatgagaacggatggctgatgtatgttcaacgtagagagtggacaacagcgataggcggtaacatccagttagtagtaaacggacagatcatcacccaaggtggagccatgaccggtcagctaaaattgcagaatgggcatgttcttcaattagagtccgcatccgacaaggcgcactatattctatctaaagatggtaacaggaataactggtacattggtagagggtcagataacaacaatgactgtaccttccactcctatgtacatggtacgaccttaacactcaagcaggactatgcagtagttaacaaacacttccacgtaggtcaggccgttgtggccactgatggtaatattcaaggtactaagtggggaggtaaatggctggatgcttacctacgtgacagcttcgttgcgaagtccaaggcgtggactcaggtgtggtctggtagtgctggcggtggggtaagtgtgactgtttcacaggatctccgcttccgcaatatctggattaagtgtgccaacaactcttggaacttcttccgtactggccccgatggaatctacttcatagcctctgatggtggatggttacgattccaaatacactccaacggtctcggattcaagaatattgcagacagtcgttcagtacctaatgcaatcatggtggagaacgagtaacgatcg BBa_Z0109_sequence 1 atgatgaacattaagactaacccgtttaaagccgtgtctttcgtagagtctgccattaagaaggctctggataacgctgggtatcttatcgctgaaatcaagtacgatggtgtacgcgggaacatctgcgtagacaatactgctaacagttactggctctctcgtgtatctaaaacgattccggcactggagcacttaaacgggtttgatgttcgctggaagcgtctactgaacgatgaccgttgcttctacaaagatggctttatgcttgatggggaactcatggtcaagggcgtagactttaacacagggtccggcctactgcgtaccaaatggactgacacgaagaaccaagagttccatgaagagttattcgttgaaccaatccgtaagaaagataaagttccctttaagctgcacactggacaccttcacataaaactgtacgctatcctcccgctgcacatcgtggagtctggagaagactgtgatgtcatgacgttgctcatgcaggaacacgttaagaacatgctgcctctgctacaggaatacttccctgaaatcgaatggcaagcggctgaatcttacgaggtctacgatatggtagaactacagcaactgtacgagcagaagcgagcagaaggccatgagggtctcattgtgaaagacccgatgtgtatctataagcgcggtaagaaatctggctggtggaaaatgaaacctgagaacgaagctgacggtatcattcagggtctggtatggggtacaaaaggtctggctaatgaaggtaaagtgattggttttgaggtgcttcttgagagtggtcgtttagttaacgccacgaatatctctcgcgccttaatggatgagttcactgagacagtaaaagaggccaccctaagtcaatggggattctttagcccatacggtattggcgacaacgatgcttgtactattaacccttacgatggctgggcgtgtcaaattagctacatggaggaaacacctgatggctctttgcggcacccatcgttcgtaatgttccgtggcaccgaggacaaccctcaagagaaaatgtaacggccg BBa_Z0253_sequence 1 agcaccgaagtaatacgactcactattagggaaga BBa_Z0157_sequence 1 gtgctatcattagactttaacaacgaattgattaaggctgctccaattgttgggacgggtgtagcagatgttagtgctcgactgttctttgggttaagccttaacgaatggttctacgttgctgctatcgcctacacagtggttcagattggtgccaaggtagtcgataagatgattgactggaagaaagccaataaagaatga BBa_Z0121_sequence 1 atgatcgtaagtgatatagaagcgaatgcattattagaaagcgtcactaagttccactgcggggttatctacgactactccaccgctgagtacgtaagctaccgtccgagtgacttcggtgcgtatctggatgcgctggaagccgaggttgcacgaggcggtcttattgtgttccacaacggtcacaagtatgacgttcctgcattgaccaaactggcaaagttgcaattgaaccgagagttccaccttcctcgtgagaactgtattgacacccttgtgttgtcacgtttgattcattccaacctcaaggacaccgatatgggtcttctgcgttccggcaagttgcccggaaaacgctttgggtctcacgctttggaggcgtggggttatcgcttaggcgagatgaagggtgaatacaaagacgactttaagcgtatgcttgaagagcagggtgaagaatacgttgacggaatggagtggtggaacttcaacgaagagatgatggactataacgttcaggacgttgtggtaactaaagctctccttgagaagctactctctgacaaacattacttccctcctgagattgactttacggacgtaggatacactacgttctggtcagaatcccttgaggccgttgacattgaacatcgtgctgcatggctgctcgctaaacaagagcgcaacgggttcccgtttgacacaaaagcaatcgaagagttgtacgtagagttagctgctcgccgctctgagttgctccgtaaattgaccgaaacgttcggctcgtggtatcagcctaaaggtggcactgagatgttctgccatccgcgaacaggtaagccactacctaaataccctcgcattaagacacctaaagttggtggtatctttaagaagcctaagaacaaggcacagcgagaaggccgtgagccttgcgaacttgatacccgcgagtacgttgctggtgctccttacaccccagttgaacatgttgtgtttaacccttcgtctcgtgaccacattcagaagaaactccaagaggctgggtgggtcccgaccaagtacaccgataagggtgctcctgtggtggacgatgaggtactcgaaggagtacgtgtagatgaccctgagaagcaagccgctatcgacctcattaaagagtacttgatgattcagaagcgaatcggacagtctgctgagggagacaaagcatggcttcgttatgttgctgaggatggtaagattcatggttctgttaaccctaatggagcagttacgggtcgtgcgacccatgcgttcccaaaccttgcgcaaattccgggtgtacgttctccttatggagagcagtgtcgcgctgcttttggcgctgagcaccatttggatgggataactggtaagccttgggttcaggctggcatcgacgcatccggtcttgagctacgctgcttggctcacttcatggctcgctttgataacggcgagtacgctcacgagattcttaacggcgacatccacactaagaaccagatagctgctgaactacctacccgagataacgctaagacgttcatctatgggttcctctatggtgctggtgatgagaagattggacagattgttggtgctggtaaagagcgcggtaaggaactcaagaagaaattccttgagaacacccccgcgattgcagcactccgcgagtctatccaacagacacttgtcgagtcctctcaatgggtagctggtgagcaacaagtcaagtggaaacgccgctggattaaaggtctggatggtcgtaaggtacacgttcgtagtcctcacgctgccttgaataccctactgcaatctgctggtgctctcatctgcaaactgtggattatcaagaccgaagagatgctcgtagagaaaggcttgaagcatggctgggatggggactttgcgtacatggcatgggtacatgatgaaatccaagtaggctgccgtaccgaagagattgctcaggtggtcattgagaccgcacaagaagcgatgcgctgggttggagaccactggaacttccggtgtcttctggataccgaaggtaagatgggtcctaattgggcgatttgccactgactgcag BBa_Z0119_sequence 1 atggctcgtgtacagtttaaacaacgtgaatctactgacgcaatctttgttcactgctcggctaccaagccaagtcagaatgttggtgtccgtgagattcgccagtggcacaaagagcagggctggctcgatgtgggataccacttcatcatcaagcgagacggtactgtggaggcaggacgagatgagatggctgtaggctctcacgctaagggttacaaccacaactctatcggcgtctgccttgttggtggtatcgacgataaaggtaagttcgacgctaactttacgccagcccaaatgcaatcccttcgctcactgcttgtcacactgctggctaagtacgaaggcgctgtgcttcgcgcccatcatgaggtggcgccgaaggcttgcccttcgttcgaccttaagcgttggtgggagaagaacgaactggtcacttctgaccgtggataagatct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z