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display id: BBa_Z0503
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was generated by: iGEM to SBOL conversion
description: T7.2 revision 3
created: 2006-06-21T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: Designs by Alex Mallet and Sri Kosuri
owned by: james
owned by: myers
mutable description: Fixed secondary structure on venus yfp and rbs.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
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mutable notes: see http://openwetware.org/wiki/T7.2
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creator: Sriram Kosuri
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persistent identity: range2243159
display id: range2243159
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 34098
end: 34117
orientation: inline

BBa_Z0144
type: SequenceAnnotation
persistent identity: annotation2243165
display id: annotation2243165
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243165/range2243165/1
component: BBa_Z0144

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243165/range2243165/1
type: Range
persistent identity: range2243165
display id: range2243165
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 34118
end: 34554
orientation: inline

BBa_Z0262
type: SequenceAnnotation
persistent identity: annotation2243166
display id: annotation2243166
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243166/range2243166/1
component: BBa_Z0262

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243166/range2243166/1
type: Range
persistent identity: range2243166
display id: range2243166
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 34555
end: 34574
orientation: inline

BBa_Z0137
type: SequenceAnnotation
persistent identity: annotation2243170
display id: annotation2243170
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243170/range2243170/1
component: BBa_Z0137

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243170/range2243170/1
type: Range
persistent identity: range2243170
display id: range2243170
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 34575
end: 36335
orientation: inline

BBa_Z0251
type: SequenceAnnotation
persistent identity: annotation2243172
display id: annotation2243172
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243172/range2243172/1
component: BBa_Z0251

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243172/range2243172/1
type: Range
persistent identity: range2243172
display id: range2243172
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 36336
end: 36370
orientation: inline

BBa_Z0261
type: SequenceAnnotation
persistent identity: annotation2243173
display id: annotation2243173
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243173/range2243173/1
component: BBa_Z0261

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243173/range2243173/1
type: Range
persistent identity: range2243173
display id: range2243173
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 36371
end: 36390
orientation: inline

BBa_Z0148
type: SequenceAnnotation
persistent identity: annotation2243174
display id: annotation2243174
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243174/range2243174/1
component: BBa_Z0148

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243174/range2243174/1
type: Range
persistent identity: range2243174
display id: range2243174
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 36391
end: 36540
orientation: inline

BBa_Z0293
type: SequenceAnnotation
persistent identity: annotation2243177
display id: annotation2243177
version: 1
top level: BBa_Z0503
location: https://synbiohub.org/public/igem/BBa_Z0503/annotation2243177/range2243177/1
component: BBa_Z0293

https://synbiohub.org/public/igem/BBa_Z0503/annotation2243177/range2243177/1
type: Range
persistent identity: range2243177
display id: range2243177
version: 1
top level: BBa_Z0503
direction: direction/reverse
start: 36541
end: 36939
orientation: inline

BBa_Z0252
type: ComponentDefinition
persistent identity: BBa Z0252
display id: BBa_Z0252
version: 1
was generated by: iGEM to SBOL conversion
description: T7 weak binding and processivity
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: T7
owned by: james
owned by: myers
mutable description: Promoter for T7 RNA polymerase
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: 23bp conserved region
sequence annotation: tga -> acg mutation from consensus
sequence annotation: t -> a mutation from consensus
sequence: https://synbiohub.org/public/igem/BBa_Z0252_sequence/1

23bp conserved region
type: SequenceAnnotation
persistent identity: annotation1476343
display id: annotation1476343
version: 1
top level: BBa_Z0252
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
type: Range
persistent identity: range1476343
display id: range1476343
version: 1
top level: BBa_Z0252
direction: direction/forward
start: 6
end: 28
orientation: inline

tga -> acg mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476344
display id: annotation1476344
version: 1
top level: BBa_Z0252
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
type: Range
persistent identity: range1476344
display id: range1476344
version: 1
top level: BBa_Z0252
direction: direction/forward
start: 10
end: 12
orientation: inline

t -> a mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476345
display id: annotation1476345
version: 1
top level: BBa_Z0252
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
type: Range
persistent identity: range1476345
display id: range1476345
version: 1
top level: BBa_Z0252
direction: direction/forward
start: 21
end: 21
orientation: inline

BBa_Z0106
type: ComponentDefinition
persistent identity: BBa Z0106
display id: BBa_Z0106
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 0.7
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:54Z
mutable provenance: T7 genome reannotation
owned by: james
owned by: myers
mutable description: Gene 0.7 codes for a protein that inactivates host-catalyzed transcription, it is also a serine-threonine protein kinase. The two activities are separable. Gp0.7 phosphorylates many host proteins, including ribosome components, ribonucleases III and E, and the β and β?? subunits of E. coli RNAP. The BR3 and Y49 strains contain the rpoC-E1158K mutation, which renders gp0.7 essential for phage growth. The mutation affects the region of β?? to which the T7 protein gp2 binds. Gene 0.7 is non-essential in wild-type hosts and is actually detrimental when T7 is grown at 30˚C in rich media. Early T7 RNA and protein synthesis is prolonged after infection by gp0.7 mutants. However, phage growth at elevated temperatures or in minimal media, or in cells harboring the Col Ib plasmid renders gene 0.7 essential. The processes underlying the conditional necessity for gp0.7 function have not been determined. The E. coli C promoter -35 region is located on the 3' end of the coding sequence. Removing the downstream region will probably be sufficient to delete functioning of the promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: -45 region of C promoter
sequence annotation: -35 region of C promoter
sequence annotation: added BsiWI
sequence: https://synbiohub.org/public/igem/BBa_Z0106_sequence/1

-45 region of C promoter
type: SequenceAnnotation
persistent identity: annotation1476456
display id: annotation1476456
version: 1
top level: BBa_Z0106
location: https://synbiohub.org/public/igem/BBa_Z0106/annotation1476456/range1476456/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0106/annotation1476456/range1476456/1
type: Range
persistent identity: range1476456
display id: range1476456
version: 1
top level: BBa_Z0106
direction: direction/forward
start: 1049
end: 1049
orientation: inline

-35 region of C promoter
type: SequenceAnnotation
persistent identity: annotation1476455
display id: annotation1476455
version: 1
top level: BBa_Z0106
location: https://synbiohub.org/public/igem/BBa_Z0106/annotation1476455/range1476455/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0106/annotation1476455/range1476455/1
type: Range
persistent identity: range1476455
display id: range1476455
version: 1
top level: BBa_Z0106
direction: direction/forward
start: 1058
end: 1062
orientation: inline

added BsiWI
type: SequenceAnnotation
persistent identity: annotation1883644
display id: annotation1883644
version: 1
top level: BBa_Z0106
location: https://synbiohub.org/public/igem/BBa_Z0106/annotation1883644/range1883644/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0106/annotation1883644/range1883644/1
type: Range
persistent identity: range1883644
display id: range1883644
version: 1
top level: BBa_Z0106
direction: direction/forward
start: 1081
end: 1086
orientation: inline

BBa_Z0107
type: ComponentDefinition
persistent identity: BBa Z0107
display id: BBa_Z0107
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 1
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:54Z
mutable provenance: T7 genome reannotation
owned by: james
owned by: myers
mutable description: Encodes gp1: T7 RNA polymerase from wild-type T7; Suppressor mutation in 19 allows no gene 1 late in infection. It's thought that gene 1 is hanging out at the CJ terminator at the pause site... recruiting 18 and 19 after packaging. (Studier FW, PC)
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aac...gac->aat..gat
sequence annotation: gttgac -> GTAGAT
sequence annotation: S1 -35 site
sequence annotation: gat -> GAC
sequence annotation: S1 -10 site
sequence annotation: S1 promoter startsite
sequence annotation: added BspEI
sequence: https://synbiohub.org/public/igem/BBa_Z0107_sequence/1

aac...gac->aat..gat
type: SequenceAnnotation
persistent identity: annotation1881041
display id: annotation1881041
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1881041/range1881041/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0107/annotation1881041/range1881041/1
type: Range
persistent identity: range1881041
display id: range1881041
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 4
end: 39
orientation: inline

gttgac -> GTAGAT
type: SequenceAnnotation
persistent identity: annotation1860879
display id: annotation1860879
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1860879/range1860879/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0107/annotation1860879/range1860879/1
type: Range
persistent identity: range1860879
display id: range1860879
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 2497
end: 2502
orientation: inline

S1 -35 site
type: SequenceAnnotation
persistent identity: annotation1476462
display id: annotation1476462
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1476462/range1476462/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_Z0107/annotation1476462/range1476462/1
type: Range
persistent identity: range1476462
display id: range1476462
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 2498
end: 2502
orientation: inline

gat -> GAC
type: SequenceAnnotation
persistent identity: annotation1860880
display id: annotation1860880
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1860880/range1860880/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0107/annotation1860880/range1860880/1
type: Range
persistent identity: range1860880
display id: range1860880
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 2518
end: 2520
orientation: inline

S1 -10 site
type: SequenceAnnotation
persistent identity: annotation1476461
display id: annotation1476461
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1476461/range1476461/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0107/annotation1476461/range1476461/1
type: Range
persistent identity: range1476461
display id: range1476461
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 2520
end: 2525
orientation: inline

S1 promoter startsite
type: SequenceAnnotation
persistent identity: annotation1476460
display id: annotation1476460
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1476460/range1476460/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0107/annotation1476460/range1476460/1
type: Range
persistent identity: range1476460
display id: range1476460
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 2532
end: 2532
orientation: inline

added BspEI
type: SequenceAnnotation
persistent identity: annotation1883668
display id: annotation1883668
version: 1
top level: BBa_Z0107
location: https://synbiohub.org/public/igem/BBa_Z0107/annotation1883668/range1883668/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0107/annotation1883668/range1883668/1
type: Range
persistent identity: range1883668
display id: range1883668
version: 1
top level: BBa_Z0107
direction: direction/forward
start: 2653
end: 2658
orientation: inline

BBa_Z0108
type: ComponentDefinition
persistent identity: BBa Z0108
display id: BBa_Z0108
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 1.1
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:54Z
mutable provenance: T7 genome reannotation
owned by: james
owned by: myers
mutable description: open reading frame for gene 1.1. from wild-type T7 genome
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_Z0108_sequence/1

BBa_Z0115
type: ComponentDefinition
persistent identity: BBa Z0115
display id: BBa_Z0115
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 1.2
created: 2006-04-11T11:00:00Z
modified: 2015-05-08T01:14:54Z
mutable provenance: T7 genome reannotation
owned by: james
owned by: myers
mutable description: Gene 1.2 inhibits E. coli dGTPase and converts it into a rGTP-binding protein, which is important for T7 only when dgt is over-expressed (optA1 mutation). T7 gene 1.2 also causes phage exclusion by the F plasmid, but T3 gene 1.2 overcomes the exclusion system.
discontinued: false
dominant: false
group u list: _10_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_Z0115_sequence/1

BBa_Z0151
type: ComponentDefinition
persistent identity: BBa Z0151
display id: BBa_Z0151
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 1.7
created: 2006-04-26T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Bases have been changed to remove gene 1.8 start site acataaAgataaatgttaCgcataa
discontinued: false
dominant: false
group u list: _10_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: g->A to remove gene 1.8 RBS
sequence annotation: t->C to remove gene 1.8 start
sequence: https://synbiohub.org/public/igem/BBa_Z0151_sequence/1

g->A to remove gene 1.8 RBS
type: SequenceAnnotation
persistent identity: annotation1849888
display id: annotation1849888
version: 1
top level: BBa_Z0151
location: https://synbiohub.org/public/igem/BBa_Z0151/annotation1849888/range1849888/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0151/annotation1849888/range1849888/1
type: Range
persistent identity: range1849888
display id: range1849888
version: 1
top level: BBa_Z0151
direction: direction/forward
start: 573
end: 573
orientation: inline

t->C to remove gene 1.8 start
type: SequenceAnnotation
persistent identity: annotation1849887
display id: annotation1849887
version: 1
top level: BBa_Z0151
location: https://synbiohub.org/public/igem/BBa_Z0151/annotation1849887/range1849887/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0151/annotation1849887/range1849887/1
type: Range
persistent identity: range1849887
display id: range1849887
version: 1
top level: BBa_Z0151
direction: direction/forward
start: 585
end: 585
orientation: inline

BBa_Z0158
type: ComponentDefinition
persistent identity: BBa Z0158
display id: BBa_Z0158
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 0.3
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: 3' end of gene mutated to remove gene 0.4 RBS
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: t->C mutation
sequence annotation: add apaI
sequence: https://synbiohub.org/public/igem/BBa_Z0158_sequence/1

t->C mutation
type: SequenceAnnotation
persistent identity: annotation1855703
display id: annotation1855703
version: 1
top level: BBa_Z0158
location: https://synbiohub.org/public/igem/BBa_Z0158/annotation1855703/range1855703/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0158/annotation1855703/range1855703/1
type: Range
persistent identity: range1855703
display id: range1855703
version: 1
top level: BBa_Z0158
direction: direction/forward
start: 345
end: 345
orientation: inline

add apaI
type: SequenceAnnotation
persistent identity: annotation1883643
display id: annotation1883643
version: 1
top level: BBa_Z0158
location: https://synbiohub.org/public/igem/BBa_Z0158/annotation1883643/range1883643/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0158/annotation1883643/range1883643/1
type: Range
persistent identity: range1883643
display id: range1883643
version: 1
top level: BBa_Z0158
direction: direction/forward
start: 355
end: 360
orientation: inline

BBa_Z0253
type: ComponentDefinition
persistent identity: BBa Z0253
display id: BBa_Z0253
version: 1
was generated by: iGEM to SBOL conversion
description: T7 weak binding promoter
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ag->TA from concensus
sequence annotation: ag->GA from concensus
sequence: https://synbiohub.org/public/igem/BBa_Z0253_sequence/1

ag->TA from concensus
type: SequenceAnnotation
persistent identity: annotation1862684
display id: annotation1862684
version: 1
top level: BBa_Z0253
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
type: Range
persistent identity: range1862684
display id: range1862684
version: 1
top level: BBa_Z0253
direction: direction/forward
start: 27
end: 28
orientation: inline

ag->GA from concensus
type: SequenceAnnotation
persistent identity: annotation1862691
display id: annotation1862691
version: 1
top level: BBa_Z0253
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
type: Range
persistent identity: range1862691
display id: range1862691
version: 1
top level: BBa_Z0253
direction: direction/forward
start: 31
end: 32
orientation: inline

BBa_Z0261
type: ComponentDefinition
persistent identity: BBa Z0261
display id: BBa_Z0261
version: 1
was generated by: iGEM to SBOL conversion
description: Strong T7.2 RBS
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0261_sequence/1

BBa_Z0262
type: ComponentDefinition
persistent identity: BBa Z0262
display id: BBa_Z0262
version: 1
was generated by: iGEM to SBOL conversion
description: Medium strength T7.2 RBS
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0262_sequence/1

BBa_Z0271
type: ComponentDefinition
persistent identity: BBa Z0271
display id: BBa_Z0271
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 TE transcriptional terminator
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Takes 6bp ahead of the expected start of the stem loop, and 3bp after the expected termination site (11bp after end of stem loop). Causes termination by E. coli RNA polymerase (but not T7 RNA polymerase).
discontinued: false
dominant: false
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TE expected stem loop
sequence: https://synbiohub.org/public/igem/BBa_Z0271_sequence/1

TE expected stem loop
type: SequenceAnnotation
persistent identity: annotation1862801
display id: annotation1862801
version: 1
top level: BBa_Z0271
location: https://synbiohub.org/public/igem/BBa_Z0271/annotation1862801/range1862801/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_Z0271/annotation1862801/range1862801/1
type: Range
persistent identity: range1862801
display id: range1862801
version: 1
top level: BBa_Z0271
direction: direction/forward
start: 7
end: 26
orientation: inline

BBa_Z0291
type: ComponentDefinition
persistent identity: BBa Z0291
display id: BBa_Z0291
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 primary origin of replication
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: T7 phi1.1A promoter
sequence annotation: R1.1 stem loop
sequence annotation: T7 phi1.1B promoter
sequence: https://synbiohub.org/public/igem/BBa_Z0291_sequence/1

T7 phi1.1A promoter
type: SequenceAnnotation
persistent identity: annotation1862808
display id: annotation1862808
version: 1
top level: BBa_Z0291
location: https://synbiohub.org/public/igem/BBa_Z0291/annotation1862808/range1862808/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0291/annotation1862808/range1862808/1
type: Range
persistent identity: range1862808
display id: range1862808
version: 1
top level: BBa_Z0291
direction: direction/forward
start: 2
end: 36
orientation: inline

R1.1 stem loop
type: SequenceAnnotation
persistent identity: annotation1862810
display id: annotation1862810
version: 1
top level: BBa_Z0291
location: https://synbiohub.org/public/igem/BBa_Z0291/annotation1862810/range1862810/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_Z0291/annotation1862810/range1862810/1
type: Range
persistent identity: range1862810
display id: range1862810
version: 1
top level: BBa_Z0291
direction: direction/forward
start: 28
end: 80
orientation: inline

T7 phi1.1B promoter
type: SequenceAnnotation
persistent identity: annotation1862809
display id: annotation1862809
version: 1
top level: BBa_Z0291
location: https://synbiohub.org/public/igem/BBa_Z0291/annotation1862809/range1862809/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0291/annotation1862809/range1862809/1
type: Range
persistent identity: range1862809
display id: range1862809
version: 1
top level: BBa_Z0291
direction: direction/forward
start: 77
end: 111
orientation: inline

BBa_Z0292
type: ComponentDefinition
persistent identity: BBa Z0292
display id: BBa_Z0292
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Left End
created: 2006-05-29T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Left end of T7 genome. Includes terminal repeat, short repeats, leftward host promoter A0. Contains sequence up to -27bp of T7 promoter phi0L.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: left terminal repeat
sequence annotation: short left terminal repeat region
sequence annotation: A0
sequence: https://synbiohub.org/public/igem/BBa_Z0292_sequence/1

left terminal repeat
type: SequenceAnnotation
persistent identity: annotation1875186
display id: annotation1875186
version: 1
top level: BBa_Z0292
location: https://synbiohub.org/public/igem/BBa_Z0292/annotation1875186/range1875186/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_Z0292/annotation1875186/range1875186/1
type: Range
persistent identity: range1875186
display id: range1875186
version: 1
top level: BBa_Z0292
direction: direction/forward
start: 1
end: 160
orientation: inline

short left terminal repeat region
type: SequenceAnnotation
persistent identity: annotation1875187
display id: annotation1875187
version: 1
top level: BBa_Z0292
location: https://synbiohub.org/public/igem/BBa_Z0292/annotation1875187/range1875187/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_Z0292/annotation1875187/range1875187/1
type: Range
persistent identity: range1875187
display id: range1875187
version: 1
top level: BBa_Z0292
direction: direction/forward
start: 175
end: 338
orientation: inline

A0
type: SequenceAnnotation
persistent identity: annotation1875188
display id: annotation1875188
version: 1
top level: BBa_Z0292
location: https://synbiohub.org/public/igem/BBa_Z0292/annotation1875188/range1875188/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0292/annotation1875188/range1875188/1
type: Range
persistent identity: range1875188
display id: range1875188
version: 1
top level: BBa_Z0292
direction: direction/reverse
start: 210
end: 272
orientation: inline

BBa_Z0294
type: ComponentDefinition
persistent identity: BBa Z0294
display id: BBa_Z0294
version: 1
was generated by: iGEM to SBOL conversion
description: A1,A2,A3,boxA
created: 2006-05-29T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Host promoter region containing 3 promoters and boxA antitermination site. Takes natural sequence from end of phi0L to start of R0.3
discontinued: false
dominant: false
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: A1
sequence annotation: A2
sequence annotation: A3
sequence annotation: boxA antitermination site
sequence annotation: add gctc to give SacI
sequence: https://synbiohub.org/public/igem/BBa_Z0294_sequence/1

A1
type: SequenceAnnotation
persistent identity: annotation1875191
display id: annotation1875191
version: 1
top level: BBa_Z0294
location: https://synbiohub.org/public/igem/BBa_Z0294/annotation1875191/range1875191/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0294/annotation1875191/range1875191/1
type: Range
persistent identity: range1875191
display id: range1875191
version: 1
top level: BBa_Z0294
direction: direction/forward
start: 37
end: 99
orientation: inline

A2
type: SequenceAnnotation
persistent identity: annotation1875192
display id: annotation1875192
version: 1
top level: BBa_Z0294
location: https://synbiohub.org/public/igem/BBa_Z0294/annotation1875192/range1875192/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0294/annotation1875192/range1875192/1
type: Range
persistent identity: range1875192
display id: range1875192
version: 1
top level: BBa_Z0294
direction: direction/forward
start: 164
end: 227
orientation: inline

A3
type: SequenceAnnotation
persistent identity: annotation1875193
display id: annotation1875193
version: 1
top level: BBa_Z0294
location: https://synbiohub.org/public/igem/BBa_Z0294/annotation1875193/range1875193/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0294/annotation1875193/range1875193/1
type: Range
persistent identity: range1875193
display id: range1875193
version: 1
top level: BBa_Z0294
direction: direction/forward
start: 289
end: 351
orientation: inline

boxA antitermination site
type: SequenceAnnotation
persistent identity: annotation1875194
display id: annotation1875194
version: 1
top level: BBa_Z0294
location: https://synbiohub.org/public/igem/BBa_Z0294/annotation1875194/range1875194/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_Z0294/annotation1875194/range1875194/1
type: Range
persistent identity: range1875194
display id: range1875194
version: 1
top level: BBa_Z0294
direction: direction/forward
start: 400
end: 409
orientation: inline

add gctc to give SacI
type: SequenceAnnotation
persistent identity: annotation1883627
display id: annotation1883627
version: 1
top level: BBa_Z0294
location: https://synbiohub.org/public/igem/BBa_Z0294/annotation1883627/range1883627/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0294/annotation1883627/range1883627/1
type: Range
persistent identity: range1883627
display id: range1883627
version: 1
top level: BBa_Z0294
direction: direction/forward
start: 432
end: 435
orientation: inline

BBa_Z0273
type: ComponentDefinition
persistent identity: BBa Z0273
display id: BBa_Z0273
version: 1
was generated by: iGEM to SBOL conversion
description: T7 R0.3 RNAseIII site
created: 2006-05-29T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Contains 9 bp before and 10 bp after stem loop(up to start of 0.3 RBS).
discontinued: false
dominant: false
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: R0.3 stem loop
sequence: https://synbiohub.org/public/igem/BBa_Z0273_sequence/1

R0.3 stem loop
type: SequenceAnnotation
persistent identity: annotation1875207
display id: annotation1875207
version: 1
top level: BBa_Z0273
location: https://synbiohub.org/public/igem/BBa_Z0273/annotation1875207/range1875207/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_Z0273/annotation1875207/range1875207/1
type: Range
persistent identity: range1875207
display id: range1875207
version: 1
top level: BBa_Z0273
direction: direction/forward
start: 10
end: 58
orientation: inline

BBa_Z0301
type: ComponentDefinition
persistent identity: BBa Z0301
display id: BBa_Z0301
version: 1
was generated by: iGEM to SBOL conversion
description: Venus YFP - E. coli codon optimized
created: 2006-06-20T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab.
owned by: james
owned by: myers
mutable description: Brighter Variant than standard EYFP.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 10
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None really
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: gtc ...gtt -> gta ... gtt codon shuffle
sequence: https://synbiohub.org/public/igem/BBa_Z0301_sequence/1

gtc ...gtt -> gta ... gtt codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881736
display id: annotation1881736
version: 1
top level: BBa_Z0301
location: https://synbiohub.org/public/igem/BBa_Z0301/annotation1881736/range1881736/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0301/annotation1881736/range1881736/1
type: Range
persistent identity: range1881736
display id: range1881736
version: 1
top level: BBa_Z0301
direction: direction/forward
start: 4
end: 39
orientation: inline

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: iGEM to SBOL conversion
description: T7 strong promoter
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
mutable description: Promoter for T7 RNA polymerase
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
top level: BBa_Z0251
direction: direction/forward
start: 6
end: 28
orientation: inline

BBa_Z0109
type: ComponentDefinition
persistent identity: BBa Z0109
display id: BBa_Z0109
version: 1
was generated by: iGEM to SBOL conversion
description: T7 gene 1.3
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:54Z
mutable provenance: T7 genome reannotation
owned by: james
owned by: myers
mutable description: The ATP-dependent DNA ligase is only essential in E. coli lig mutants, but its presence enhances phage growth in wild-type hosts.
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T7.2
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: added eagI
sequence: https://synbiohub.org/public/igem/BBa_Z0109_sequence/1

added eagI
type: SequenceAnnotation
persistent identity: annotation1883669
display id: annotation1883669
version: 1
top level: BBa_Z0109
location: https://synbiohub.org/public/igem/BBa_Z0109/annotation1883669/range1883669/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0109/annotation1883669/range1883669/1
type: Range
persistent identity: range1883669
display id: range1883669
version: 1
top level: BBa_Z0109
direction: direction/forward
start: 1081
end: 1086
orientation: inline

BBa_Z0116
type: ComponentDefinition
persistent identity: BBa Z0116
display id: BBa_Z0116
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 2
created: 2006-04-11T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Gp2 binds to and is an inhibitor of E. coli RNAP but in K-12 or B strains the defects due to a lack of gene 2 activity are reduced DNA replication and premature breakdown of replicating DNA, specifically at the left genome end where the major E. coli promoters are found.
discontinued: false
dominant: false
group u list: _10_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tca...aat->agc...aat
sequence annotation: added ecoRI
sequence: https://synbiohub.org/public/igem/BBa_Z0116_sequence/1

tca...aat->agc...aat
type: SequenceAnnotation
persistent identity: annotation1881250
display id: annotation1881250
version: 1
top level: BBa_Z0116
location: https://synbiohub.org/public/igem/BBa_Z0116/annotation1881250/range1881250/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0116/annotation1881250/range1881250/1
type: Range
persistent identity: range1881250
display id: range1881250
version: 1
top level: BBa_Z0116
direction: direction/forward
start: 4
end: 39
orientation: inline

added ecoRI
type: SequenceAnnotation
persistent identity: annotation1883670
display id: annotation1883670
version: 1
top level: BBa_Z0116
location: https://synbiohub.org/public/igem/BBa_Z0116/annotation1883670/range1883670/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0116/annotation1883670/range1883670/1
type: Range
persistent identity: range1883670
display id: range1883670
version: 1
top level: BBa_Z0116
direction: direction/forward
start: 196
end: 201
orientation: inline

BBa_Z0118
type: ComponentDefinition
persistent identity: BBa Z0118
display id: BBa_Z0118
version: 1
was generated by: iGEM to SBOL conversion
description: T7 gene 3
created: 2006-04-24T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Encodes for a endonuclease that prefers ssDNA. It functions along with gene 6 to degrade the host chromosome during infection and provide nucleotides for subsequent phage DNA replication. Gene 3 is also a Holliday junction resolvase.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_Z0118_sequence/1

BBa_Z0119
type: ComponentDefinition
persistent identity: BBa Z0119
display id: BBa_Z0119
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 3.5
created: 2006-04-24T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Gene 3.5 codes for lysozyme, which is actually not a lysozyme but an N-acetylmuramyl-L-alanine amidase. The most important functions of lysozyme are also not in cell lysis but lie in controlling initiation and termination by T7 RNAP, which in turn affect DNA replication and packaging.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: S3: possible promoter
sequence annotation: ggt???>GGC
sequence annotation: S3 -35 region
sequence annotation: ttt->TTC
sequence annotation: S3 -10 region
sequence: https://synbiohub.org/public/igem/BBa_Z0119_sequence/1

S3: possible promoter
type: SequenceAnnotation
persistent identity: annotation1849938
display id: annotation1849938
version: 1
top level: BBa_Z0119
location: https://synbiohub.org/public/igem/BBa_Z0119/annotation1849938/range1849938/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0119/annotation1849938/range1849938/1
type: Range
persistent identity: range1849938
display id: range1849938
version: 1
top level: BBa_Z0119
direction: direction/forward
start: 110
end: 173
orientation: inline

ggt???>GGC
type: SequenceAnnotation
persistent identity: annotation1862116
display id: annotation1862116
version: 1
top level: BBa_Z0119
location: https://synbiohub.org/public/igem/BBa_Z0119/annotation1862116/range1862116/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0119/annotation1862116/range1862116/1
type: Range
persistent identity: range1862116
display id: range1862116
version: 1
top level: BBa_Z0119
direction: direction/forward
start: 121
end: 123
orientation: inline

S3 -35 region
type: SequenceAnnotation
persistent identity: annotation1860956
display id: annotation1860956
version: 1
top level: BBa_Z0119
location: https://synbiohub.org/public/igem/BBa_Z0119/annotation1860956/range1860956/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0119/annotation1860956/range1860956/1
type: Range
persistent identity: range1860956
display id: range1860956
version: 1
top level: BBa_Z0119
direction: direction/forward
start: 123
end: 127
orientation: inline

ttt->TTC
type: SequenceAnnotation
persistent identity: annotation1862117
display id: annotation1862117
version: 1
top level: BBa_Z0119
location: https://synbiohub.org/public/igem/BBa_Z0119/annotation1862117/range1862117/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0119/annotation1862117/range1862117/1
type: Range
persistent identity: range1862117
display id: range1862117
version: 1
top level: BBa_Z0119
direction: direction/forward
start: 145
end: 147
orientation: inline

S3 -10 region
type: SequenceAnnotation
persistent identity: annotation1860957
display id: annotation1860957
version: 1
top level: BBa_Z0119
location: https://synbiohub.org/public/igem/BBa_Z0119/annotation1860957/range1860957/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0119/annotation1860957/range1860957/1
type: Range
persistent identity: range1860957
display id: range1860957
version: 1
top level: BBa_Z0119
direction: direction/forward
start: 147
end: 152
orientation: inline

BBa_Z0120
type: ComponentDefinition
persistent identity: BBa Z0120
display id: BBa_Z0120
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 4A/4B
created: 2006-04-24T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: The full-length gene 4 protein is a 5??->3?? helicase of the E. coli DnaB family and a DNA primase. An in-frame internal start site within gene 4 results in a protein, gp4B, that lacks the N-terminal 63 amino acids of the 63 kDa gp4A, and consequently lacks primase activity. The primase recognition sites on ssDNA are 5??-G/TGGTC, and 5??-GTGTC.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: gene 4A
sequence annotation: gac...tac -> gat ... tat codon shuffle
sequence annotation: gat->gac 4.1 start
sequence annotation: gene 4.1
sequence annotation: gene 4B
sequence annotation: S4: possible promoter
sequence annotation: ttt->TTC S4 -35
sequence annotation: tta->CUG S4-10
sequence annotation: codon shuffled (13 changes) phi4c
sequence annotation: phi4c
sequence annotation: cat->CAC 4.2 start
sequence annotation: gene 4.2
sequence annotation: added ngoMIV
sequence: https://synbiohub.org/public/igem/BBa_Z0120_sequence/1

gene 4A
type: SequenceAnnotation
persistent identity: annotation1849942
display id: annotation1849942
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1849942/range1849942/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_Z0120/annotation1849942/range1849942/1
type: Range
persistent identity: range1849942
display id: range1849942
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 1
end: 1701
orientation: inline

gac...tac -> gat ... tat codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881252
display id: annotation1881252
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1881252/range1881252/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0120/annotation1881252/range1881252/1
type: Range
persistent identity: range1881252
display id: range1881252
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 4
end: 39
orientation: inline

gat->gac 4.1 start
type: SequenceAnnotation
persistent identity: annotation1862190
display id: annotation1862190
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1862190/range1862190/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0120/annotation1862190/range1862190/1
type: Range
persistent identity: range1862190
display id: range1862190
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 70
end: 72
orientation: inline

gene 4.1
type: SequenceAnnotation
persistent identity: annotation1849947
display id: annotation1849947
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1849947/range1849947/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0120/annotation1849947/range1849947/1
type: Range
persistent identity: range1849947
display id: range1849947
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 71
end: 193
orientation: inline

gene 4B
type: SequenceAnnotation
persistent identity: annotation1849946
display id: annotation1849946
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1849946/range1849946/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0120/annotation1849946/range1849946/1
type: Range
persistent identity: range1849946
display id: range1849946
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 190
end: 1701
orientation: inline

S4: possible promoter
type: SequenceAnnotation
persistent identity: annotation1849949
display id: annotation1849949
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1849949/range1849949/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0120/annotation1849949/range1849949/1
type: Range
persistent identity: range1849949
display id: range1849949
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 574
end: 637
orientation: inline

ttt->TTC S4 -35
type: SequenceAnnotation
persistent identity: annotation1862192
display id: annotation1862192
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1862192/range1862192/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0120/annotation1862192/range1862192/1
type: Range
persistent identity: range1862192
display id: range1862192
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 586
end: 588
orientation: inline

tta->CUG S4-10
type: SequenceAnnotation
persistent identity: annotation1862193
display id: annotation1862193
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1862193/range1862193/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0120/annotation1862193/range1862193/1
type: Range
persistent identity: range1862193
display id: range1862193
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 610
end: 612
orientation: inline

codon shuffled (13 changes) phi4c
type: SequenceAnnotation
persistent identity: annotation1862194
display id: annotation1862194
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1862194/range1862194/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0120/annotation1862194/range1862194/1
type: Range
persistent identity: range1862194
display id: range1862194
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 1078
end: 1116
orientation: inline

phi4c
type: SequenceAnnotation
persistent identity: annotation1849950
display id: annotation1849950
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1849950/range1849950/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0120/annotation1849950/range1849950/1
type: Range
persistent identity: range1849950
display id: range1849950
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 1080
end: 1114
orientation: inline

cat->CAC 4.2 start
type: SequenceAnnotation
persistent identity: annotation1862191
display id: annotation1862191
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1862191/range1862191/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0120/annotation1862191/range1862191/1
type: Range
persistent identity: range1862191
display id: range1862191
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 1423
end: 1425
orientation: inline

gene 4.2
type: SequenceAnnotation
persistent identity: annotation1849948
display id: annotation1849948
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1849948/range1849948/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0120/annotation1849948/range1849948/1
type: Range
persistent identity: range1849948
display id: range1849948
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 1424
end: 1762
orientation: inline

added ngoMIV
type: SequenceAnnotation
persistent identity: annotation1883672
display id: annotation1883672
version: 1
top level: BBa_Z0120
location: https://synbiohub.org/public/igem/BBa_Z0120/annotation1883672/range1883672/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0120/annotation1883672/range1883672/1
type: Range
persistent identity: range1883672
display id: range1883672
version: 1
top level: BBa_Z0120
direction: direction/forward
start: 1702
end: 1707
orientation: inline

BBa_Z0121
type: ComponentDefinition
persistent identity: BBa Z0121
display id: BBa_Z0121
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 5
created: 2006-04-24T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: T7 DNA Polymerase consists of two proteins, the gene 5 protein and the processivity factor E. coli thioredoxin. Thioredoxin forms a 1:1 complex with the T7 protein and increases its processivity by at least 1000-fold, the complex also increases the activity of the associated 5??->3?? exonuclease on dsDNA but has little effect on ssDNA exonucleolytic activity.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: atc...gag -> atc...gaa codon shuffle
sequence annotation: added pstI
sequence: https://synbiohub.org/public/igem/BBa_Z0121_sequence/1

atc...gag -> atc...gaa codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881253
display id: annotation1881253
version: 1
top level: BBa_Z0121
location: https://synbiohub.org/public/igem/BBa_Z0121/annotation1881253/range1881253/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0121/annotation1881253/range1881253/1
type: Range
persistent identity: range1881253
display id: range1881253
version: 1
top level: BBa_Z0121
direction: direction/forward
start: 4
end: 39
orientation: inline

added pstI
type: SequenceAnnotation
persistent identity: annotation1883673
display id: annotation1883673
version: 1
top level: BBa_Z0121
location: https://synbiohub.org/public/igem/BBa_Z0121/annotation1883673/range1883673/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0121/annotation1883673/range1883673/1
type: Range
persistent identity: range1883673
display id: range1883673
version: 1
top level: BBa_Z0121
direction: direction/forward
start: 2116
end: 2121
orientation: inline

BBa_Z0123
type: ComponentDefinition
persistent identity: BBa Z0123
display id: BBa_Z0123
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 6.7
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Head protein. ~18 copies per virion. Gp6.7 is not absolutely essential, morphological normal T7 particles are produced in its absence but have reduced infectivity.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_Z0123_sequence/1

BBa_Z0124
type: ComponentDefinition
persistent identity: BBa Z0124
display id: BBa_Z0124
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 7.3
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Gene 7 was originally defined by mutations that mapped between genes 6 and 8 but was subsequently shown to be two genes, 7 and 7.3. Gp7.3 is a tail protein, present in ~30 copies per particle, that is essential for infectivity though not particle formation.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: possible promoter site?
sequence annotation: att->ATC
sequence annotation: added bstEII
sequence: https://synbiohub.org/public/igem/BBa_Z0124_sequence/1

possible promoter site?
type: SequenceAnnotation
persistent identity: annotation1855232
display id: annotation1855232
version: 1
top level: BBa_Z0124
location: https://synbiohub.org/public/igem/BBa_Z0124/annotation1855232/range1855232/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0124/annotation1855232/range1855232/1
type: Range
persistent identity: range1855232
display id: range1855232
version: 1
top level: BBa_Z0124
direction: direction/forward
start: 168
end: 168
orientation: inline

att->ATC
type: SequenceAnnotation
persistent identity: annotation1883626
display id: annotation1883626
version: 1
top level: BBa_Z0124
location: https://synbiohub.org/public/igem/BBa_Z0124/annotation1883626/range1883626/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0124/annotation1883626/range1883626/1
type: Range
persistent identity: range1883626
display id: range1883626
version: 1
top level: BBa_Z0124
direction: direction/forward
start: 295
end: 297
orientation: inline

added bstEII
type: SequenceAnnotation
persistent identity: annotation1883678
display id: annotation1883678
version: 1
top level: BBa_Z0124
location: https://synbiohub.org/public/igem/BBa_Z0124/annotation1883678/range1883678/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0124/annotation1883678/range1883678/1
type: Range
persistent identity: range1883678
display id: range1883678
version: 1
top level: BBa_Z0124
direction: direction/forward
start: 301
end: 307
orientation: inline

BBa_Z0125
type: ComponentDefinition
persistent identity: BBa Z0125
display id: BBa_Z0125
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 8
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Head-tail connector. 12 copies per virion. Three possible host promoters with -35 regions at 293, 1100, 1249
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: possible promoter site?
sequence annotation: added SalI
sequence: https://synbiohub.org/public/igem/BBa_Z0125_sequence/1

possible promoter site?
type: SequenceAnnotation
persistent identity: annotation1855239
display id: annotation1855239
version: 1
top level: BBa_Z0125
location: https://synbiohub.org/public/igem/BBa_Z0125/annotation1855239/range1855239/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0125/annotation1855239/range1855239/1
type: Range
persistent identity: range1855239
display id: range1855239
version: 1
top level: BBa_Z0125
direction: direction/forward
start: 1425
end: 1425
orientation: inline

added SalI
type: SequenceAnnotation
persistent identity: annotation1883674
display id: annotation1883674
version: 1
top level: BBa_Z0125
location: https://synbiohub.org/public/igem/BBa_Z0125/annotation1883674/range1883674/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0125/annotation1883674/range1883674/1
type: Range
persistent identity: range1883674
display id: range1883674
version: 1
top level: BBa_Z0125
direction: direction/forward
start: 1612
end: 1617
orientation: inline

BBa_Z0126
type: ComponentDefinition
persistent identity: BBa Z0126
display id: BBa_Z0126
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 9
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Capsid scaffolding protein
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ggc...ggc -> ggc...ggc codon shuffle
sequence annotation: possible promoter site?
sequence: https://synbiohub.org/public/igem/BBa_Z0126_sequence/1

ggc...ggc -> ggc...ggc codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881254
display id: annotation1881254
version: 1
top level: BBa_Z0126
location: https://synbiohub.org/public/igem/BBa_Z0126/annotation1881254/range1881254/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0126/annotation1881254/range1881254/1
type: Range
persistent identity: range1881254
display id: range1881254
version: 1
top level: BBa_Z0126
direction: direction/forward
start: 4
end: 39
orientation: inline

possible promoter site?
type: SequenceAnnotation
persistent identity: annotation1855246
display id: annotation1855246
version: 1
top level: BBa_Z0126
location: https://synbiohub.org/public/igem/BBa_Z0126/annotation1855246/range1855246/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0126/annotation1855246/range1855246/1
type: Range
persistent identity: range1855246
display id: range1855246
version: 1
top level: BBa_Z0126
direction: direction/forward
start: 522
end: 522
orientation: inline

possible promoter site?
type: SequenceAnnotation
persistent identity: annotation1855247
display id: annotation1855247
version: 1
top level: BBa_Z0126
location: https://synbiohub.org/public/igem/BBa_Z0126/annotation1855247/range1855247/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0126/annotation1855247/range1855247/1
type: Range
persistent identity: range1855247
display id: range1855247
version: 1
top level: BBa_Z0126
direction: direction/forward
start: 671
end: 671
orientation: inline

BBa_Z0127
type: ComponentDefinition
persistent identity: BBa Z0127
display id: BBa_Z0127
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 10A/B
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: major/minor capsid protein. The 10B portion is non-essential, 10A-only phages seem to grow normally. ~430 copies of 10A and 16 copies of 10B in capsid (415 expected from symmetry). JJ Dunn removed various pieces of terminator.. if you remove hairpin, the gene 10 RNA is obliterated. Also, got rid of gene 10 pre-shine dalgarno and could not get translation off gene 10. (Dunn JJ, PC)
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gene 10A
sequence annotation: gene 10B
sequence annotation: added XmaI
sequence: https://synbiohub.org/public/igem/BBa_Z0127_sequence/1

gene 10A
type: SequenceAnnotation
persistent identity: annotation1850773
display id: annotation1850773
version: 1
top level: BBa_Z0127
location: https://synbiohub.org/public/igem/BBa_Z0127/annotation1850773/range1850773/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0127/annotation1850773/range1850773/1
type: Range
persistent identity: range1850773
display id: range1850773
version: 1
top level: BBa_Z0127
direction: direction/forward
start: 1
end: 1038
orientation: inline

gene 10B
type: SequenceAnnotation
persistent identity: annotation1850774
display id: annotation1850774
version: 1
top level: BBa_Z0127
location: https://synbiohub.org/public/igem/BBa_Z0127/annotation1850774/range1850774/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0127/annotation1850774/range1850774/1
type: Range
persistent identity: range1850774
display id: range1850774
version: 1
top level: BBa_Z0127
direction: direction/forward
start: 1
end: 1196
orientation: inline

added XmaI
type: SequenceAnnotation
persistent identity: annotation1883679
display id: annotation1883679
version: 1
top level: BBa_Z0127
location: https://synbiohub.org/public/igem/BBa_Z0127/annotation1883679/range1883679/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_Z0127/annotation1883679/range1883679/1
type: Range
persistent identity: range1883679
display id: range1883679
version: 1
top level: BBa_Z0127
direction: direction/forward
start: 1197
end: 1202
orientation: inline

BBa_Z0128
type: ComponentDefinition
persistent identity: BBa Z0128
display id: BBa_Z0128
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 11
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: structural tail protein of phage capsid; 12 copies per virion
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cgc...gag ->cga...gaa codon shuffle
sequence: https://synbiohub.org/public/igem/BBa_Z0128_sequence/1

cgc...gag ->cga...gaa codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881255
display id: annotation1881255
version: 1
top level: BBa_Z0128
location: https://synbiohub.org/public/igem/BBa_Z0128/annotation1881255/range1881255/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0128/annotation1881255/range1881255/1
type: Range
persistent identity: range1881255
display id: range1881255
version: 1
top level: BBa_Z0128
direction: direction/forward
start: 4
end: 39
orientation: inline

BBa_Z0138
type: ComponentDefinition
persistent identity: BBa Z0138
display id: BBa_Z0138
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 4.3
created: 2006-04-26T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: conserved; non-essential
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_Z0138_sequence/1

BBa_Z0139
type: ComponentDefinition
persistent identity: BBa Z0139
display id: BBa_Z0139
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 4.5
created: 2006-04-26T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: conserved; non-essential
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_Z0139_sequence/1

BBa_Z0152
type: ComponentDefinition
persistent identity: BBa Z0152
display id: BBa_Z0152
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 2.5
created: 2006-04-26T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: mutation from T7 to remove RBS from gene 2.8
discontinued: false
dominant: false
group u list: _10_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: S2: possible promoter
sequence annotation: ggc...gct->ggc..gct codon shuffle
sequence annotation: ttc -> TTT
sequence annotation: S2 -35 region
sequence annotation: S2 -10 region
sequence annotation: a->G mutation to remove RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0152_sequence/1

S2: possible promoter
type: SequenceAnnotation
persistent identity: annotation1849934
display id: annotation1849934
version: 1
top level: BBa_Z0152
location: https://synbiohub.org/public/igem/BBa_Z0152/annotation1849934/range1849934/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0152/annotation1849934/range1849934/1
type: Range
persistent identity: range1849934
display id: range1849934
version: 1
top level: BBa_Z0152
direction: direction/forward
start: 3
end: 66
orientation: inline

ggc...gct->ggc..gct codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881251
display id: annotation1881251
version: 1
top level: BBa_Z0152
location: https://synbiohub.org/public/igem/BBa_Z0152/annotation1881251/range1881251/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0152/annotation1881251/range1881251/1
type: Range
persistent identity: range1881251
display id: range1881251
version: 1
top level: BBa_Z0152
direction: direction/forward
start: 4
end: 39
orientation: inline

ttc -> TTT
type: SequenceAnnotation
persistent identity: annotation1860895
display id: annotation1860895
version: 1
top level: BBa_Z0152
location: https://synbiohub.org/public/igem/BBa_Z0152/annotation1860895/range1860895/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0152/annotation1860895/range1860895/1
type: Range
persistent identity: range1860895
display id: range1860895
version: 1
top level: BBa_Z0152
direction: direction/forward
start: 16
end: 18
orientation: inline

S2 -35 region
type: SequenceAnnotation
persistent identity: annotation1860893
display id: annotation1860893
version: 1
top level: BBa_Z0152
location: https://synbiohub.org/public/igem/BBa_Z0152/annotation1860893/range1860893/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0152/annotation1860893/range1860893/1
type: Range
persistent identity: range1860893
display id: range1860893
version: 1
top level: BBa_Z0152
direction: direction/forward
start: 16
end: 20
orientation: inline

S2 -10 region
type: SequenceAnnotation
persistent identity: annotation1860894
display id: annotation1860894
version: 1
top level: BBa_Z0152
location: https://synbiohub.org/public/igem/BBa_Z0152/annotation1860894/range1860894/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0152/annotation1860894/range1860894/1
type: Range
persistent identity: range1860894
display id: range1860894
version: 1
top level: BBa_Z0152
direction: direction/forward
start: 40
end: 45
orientation: inline

a->G mutation to remove RBS
type: SequenceAnnotation
persistent identity: annotation1849919
display id: annotation1849919
version: 1
top level: BBa_Z0152
location: https://synbiohub.org/public/igem/BBa_Z0152/annotation1849919/range1849919/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0152/annotation1849919/range1849919/1
type: Range
persistent identity: range1849919
display id: range1849919
version: 1
top level: BBa_Z0152
direction: direction/forward
start: 690
end: 690
orientation: inline

BBa_Z0154
type: ComponentDefinition
persistent identity: BBa Z0154
display id: BBa_Z0154
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 6
created: 2006-04-30T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: 3' end mutated to remove gene 6.3 startsite and alter ribosome binding site
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: g->A mutation to alter gene 6.3 RBS
sequence annotation: g->C mutation to remove gene 6.3 start
sequence annotation: added xhoI
sequence: https://synbiohub.org/public/igem/BBa_Z0154_sequence/1

g->A mutation to alter gene 6.3 RBS
type: SequenceAnnotation
persistent identity: annotation1850751
display id: annotation1850751
version: 1
top level: BBa_Z0154
location: https://synbiohub.org/public/igem/BBa_Z0154/annotation1850751/range1850751/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0154/annotation1850751/range1850751/1
type: Range
persistent identity: range1850751
display id: range1850751
version: 1
top level: BBa_Z0154
direction: direction/forward
start: 882
end: 882
orientation: inline

g->C mutation to remove gene 6.3 start
type: SequenceAnnotation
persistent identity: annotation1850750
display id: annotation1850750
version: 1
top level: BBa_Z0154
location: https://synbiohub.org/public/igem/BBa_Z0154/annotation1850750/range1850750/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0154/annotation1850750/range1850750/1
type: Range
persistent identity: range1850750
display id: range1850750
version: 1
top level: BBa_Z0154
direction: direction/forward
start: 891
end: 891
orientation: inline

added xhoI
type: SequenceAnnotation
persistent identity: annotation1884018
display id: annotation1884018
version: 1
top level: BBa_Z0154
location: https://synbiohub.org/public/igem/BBa_Z0154/annotation1884018/range1884018/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0154/annotation1884018/range1884018/1
type: Range
persistent identity: range1884018
display id: range1884018
version: 1
top level: BBa_Z0154
direction: direction/forward
start: 904
end: 909
orientation: inline

BBa_Z0159
type: ComponentDefinition
persistent identity: BBa Z0159
display id: BBa_Z0159
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 6.5
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: silent mutation at 3' end to remove gene 6.7 RBS. -35 region of F1 probably non-functional at 5' end of gene.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: g->C mutation
sequence annotation: g->A mutation
sequence: https://synbiohub.org/public/igem/BBa_Z0159_sequence/1

g->C mutation
type: SequenceAnnotation
persistent identity: annotation1855744
display id: annotation1855744
version: 1
top level: BBa_Z0159
location: https://synbiohub.org/public/igem/BBa_Z0159/annotation1855744/range1855744/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0159/annotation1855744/range1855744/1
type: Range
persistent identity: range1855744
display id: range1855744
version: 1
top level: BBa_Z0159
direction: direction/forward
start: 246
end: 246
orientation: inline

g->A mutation
type: SequenceAnnotation
persistent identity: annotation1855743
display id: annotation1855743
version: 1
top level: BBa_Z0159
location: https://synbiohub.org/public/igem/BBa_Z0159/annotation1855743/range1855743/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0159/annotation1855743/range1855743/1
type: Range
persistent identity: range1855743
display id: range1855743
version: 1
top level: BBa_Z0159
direction: direction/forward
start: 249
end: 249
orientation: inline

BBa_Z0160
type: ComponentDefinition
persistent identity: BBa Z0160
display id: BBa_Z0160
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 5.5-5.7
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Removes the overlap of gene 5.9 RBS. Not the actual sequence in the design of T7.1, allows the frameshift again.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gene 5.5
sequence annotation: gene 5.5-5.7 fusion
sequence annotation: gene 5.7
sequence annotation: a->C mutation
sequence annotation: a->G mutation
sequence: https://synbiohub.org/public/igem/BBa_Z0160_sequence/1

gene 5.5
type: SequenceAnnotation
persistent identity: annotation1862271
display id: annotation1862271
version: 1
top level: BBa_Z0160
location: https://synbiohub.org/public/igem/BBa_Z0160/annotation1862271/range1862271/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_Z0160/annotation1862271/range1862271/1
type: Range
persistent identity: range1862271
display id: range1862271
version: 1
top level: BBa_Z0160
direction: direction/forward
start: 1
end: 300
orientation: inline

gene 5.5-5.7 fusion
type: SequenceAnnotation
persistent identity: annotation1862270
display id: annotation1862270
version: 1
top level: BBa_Z0160
location: https://synbiohub.org/public/igem/BBa_Z0160/annotation1862270/range1862270/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0160/annotation1862270/range1862270/1
type: Range
persistent identity: range1862270
display id: range1862270
version: 1
top level: BBa_Z0160
direction: direction/forward
start: 1
end: 509
orientation: inline

gene 5.7
type: SequenceAnnotation
persistent identity: annotation1862272
display id: annotation1862272
version: 1
top level: BBa_Z0160
location: https://synbiohub.org/public/igem/BBa_Z0160/annotation1862272/range1862272/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_Z0160/annotation1862272/range1862272/1
type: Range
persistent identity: range1862272
display id: range1862272
version: 1
top level: BBa_Z0160
direction: direction/forward
start: 300
end: 509
orientation: inline

a->C mutation
type: SequenceAnnotation
persistent identity: annotation1855777
display id: annotation1855777
version: 1
top level: BBa_Z0160
location: https://synbiohub.org/public/igem/BBa_Z0160/annotation1855777/range1855777/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0160/annotation1855777/range1855777/1
type: Range
persistent identity: range1855777
display id: range1855777
version: 1
top level: BBa_Z0160
direction: direction/forward
start: 497
end: 497
orientation: inline

a->G mutation
type: SequenceAnnotation
persistent identity: annotation1855776
display id: annotation1855776
version: 1
top level: BBa_Z0160
location: https://synbiohub.org/public/igem/BBa_Z0160/annotation1855776/range1855776/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0160/annotation1855776/range1855776/1
type: Range
persistent identity: range1855776
display id: range1855776
version: 1
top level: BBa_Z0160
direction: direction/forward
start: 500
end: 500
orientation: inline

BBa_Z0272
type: ComponentDefinition
persistent identity: BBa Z0272
display id: BBa_Z0272
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 Tphi transcriptional terminator
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Taken 10 bp before expected start of stem loop structure, and 3bp after expected stop of transcription (7bp after stem loop)
discontinued: false
dominant: false
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Temporary
role: SO:0000110
sequence annotation: terminator stem loop
sequence: https://synbiohub.org/public/igem/BBa_Z0272_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1862785
display id: annotation1862785
version: 1
top level: BBa_Z0272
location: https://synbiohub.org/public/igem/BBa_Z0272/annotation1862785/range1862785/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_Z0272/annotation1862785/range1862785/1
type: Range
persistent identity: range1862785
display id: range1862785
version: 1
top level: BBa_Z0272
direction: direction/forward
start: 11
end: 46
orientation: inline

BBa_Z0129
type: ComponentDefinition
persistent identity: BBa Z0129
display id: BBa_Z0129
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 12
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: a structural tail protein of the phage capsid; 6 copies per virion
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: gca...ggt->gca...ggc codon shuffle
sequence annotation: F2
sequence annotation: gtt->GTG F2-35
sequence annotation: added ttaa to form PacI
sequence: https://synbiohub.org/public/igem/BBa_Z0129_sequence/1

gca...ggt->gca...ggc codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881263
display id: annotation1881263
version: 1
top level: BBa_Z0129
location: https://synbiohub.org/public/igem/BBa_Z0129/annotation1881263/range1881263/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0129/annotation1881263/range1881263/1
type: Range
persistent identity: range1881263
display id: range1881263
version: 1
top level: BBa_Z0129
direction: direction/forward
start: 4
end: 39
orientation: inline

F2
type: SequenceAnnotation
persistent identity: annotation1881261
display id: annotation1881261
version: 1
top level: BBa_Z0129
location: https://synbiohub.org/public/igem/BBa_Z0129/annotation1881261/range1881261/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0129/annotation1881261/range1881261/1
type: Range
persistent identity: range1881261
display id: range1881261
version: 1
top level: BBa_Z0129
direction: direction/reverse
start: 337
end: 399
orientation: inline

gtt->GTG F2-35
type: SequenceAnnotation
persistent identity: annotation1881262
display id: annotation1881262
version: 1
top level: BBa_Z0129
location: https://synbiohub.org/public/igem/BBa_Z0129/annotation1881262/range1881262/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0129/annotation1881262/range1881262/1
type: Range
persistent identity: range1881262
display id: range1881262
version: 1
top level: BBa_Z0129
direction: direction/forward
start: 382
end: 384
orientation: inline

added ttaa to form PacI
type: SequenceAnnotation
persistent identity: annotation1883677
display id: annotation1883677
version: 1
top level: BBa_Z0129
location: https://synbiohub.org/public/igem/BBa_Z0129/annotation1883677/range1883677/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0129/annotation1883677/range1883677/1
type: Range
persistent identity: range1883677
display id: range1883677
version: 1
top level: BBa_Z0129
direction: direction/forward
start: 2386
end: 2389
orientation: inline

BBa_Z0132
type: ComponentDefinition
persistent identity: BBa Z0132
display id: BBa_Z0132
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 15
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: internal core protein of phage capsid; ~8 copies per virion
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_Z0132_sequence/1

BBa_Z0133
type: ComponentDefinition
persistent identity: BBa Z0133
display id: BBa_Z0133
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 16
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Internal core protein of phage capsid; ~4 copies per virion
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: S6: possible promoter
sequence annotation: ttgact->CTGACC S6-35
sequence annotation: aagatttat->AAAATCTAC S6-10
sequence: https://synbiohub.org/public/igem/BBa_Z0133_sequence/1

S6: possible promoter
type: SequenceAnnotation
persistent identity: annotation1855274
display id: annotation1855274
version: 1
top level: BBa_Z0133
location: https://synbiohub.org/public/igem/BBa_Z0133/annotation1855274/range1855274/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_Z0133/annotation1855274/range1855274/1
type: Range
persistent identity: range1855274
display id: range1855274
version: 1
top level: BBa_Z0133
direction: direction/forward
start: 3876
end: 3939
orientation: inline

ttgact->CTGACC S6-35
type: SequenceAnnotation
persistent identity: annotation1862596
display id: annotation1862596
version: 1
top level: BBa_Z0133
location: https://synbiohub.org/public/igem/BBa_Z0133/annotation1862596/range1862596/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0133/annotation1862596/range1862596/1
type: Range
persistent identity: range1862596
display id: range1862596
version: 1
top level: BBa_Z0133
direction: direction/forward
start: 3889
end: 3894
orientation: inline

aagatttat->AAAATCTAC S6-10
type: SequenceAnnotation
persistent identity: annotation1862595
display id: annotation1862595
version: 1
top level: BBa_Z0133
location: https://synbiohub.org/public/igem/BBa_Z0133/annotation1862595/range1862595/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0133/annotation1862595/range1862595/1
type: Range
persistent identity: range1862595
display id: range1862595
version: 1
top level: BBa_Z0133
direction: direction/forward
start: 3910
end: 3918
orientation: inline

BBa_Z0134
type: ComponentDefinition
persistent identity: BBa Z0134
display id: BBa_Z0134
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 17
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: T7 tail fiber protein of phage capsid (adsorption); 14 +/- 3 (eighteen expected by symmettry) copies per virion; 3 copies per tail fiber
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: added PvuI
sequence: https://synbiohub.org/public/igem/BBa_Z0134_sequence/1

added PvuI
type: SequenceAnnotation
persistent identity: annotation1884038
display id: annotation1884038
version: 1
top level: BBa_Z0134
location: https://synbiohub.org/public/igem/BBa_Z0134/annotation1884038/range1884038/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0134/annotation1884038/range1884038/1
type: Range
persistent identity: range1884038
display id: range1884038
version: 1
top level: BBa_Z0134
direction: direction/forward
start: 1663
end: 1668
orientation: inline

BBa_Z0136
type: ComponentDefinition
persistent identity: BBa Z0136
display id: BBa_Z0136
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 18
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: small terminase subunit
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: added AGCTT to form HindIII
sequence: https://synbiohub.org/public/igem/BBa_Z0136_sequence/1

added AGCTT to form HindIII
type: SequenceAnnotation
persistent identity: annotation1884021
display id: annotation1884021
version: 1
top level: BBa_Z0136
location: https://synbiohub.org/public/igem/BBa_Z0136/annotation1884021/range1884021/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0136/annotation1884021/range1884021/1
type: Range
persistent identity: range1884021
display id: range1884021
version: 1
top level: BBa_Z0136
direction: direction/forward
start: 271
end: 275
orientation: inline

BBa_Z0137
type: ComponentDefinition
persistent identity: BBa Z0137
display id: BBa_Z0137
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 19 (19.2, 19.3)
created: 2006-04-25T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: large terminase subunit. binds capsid pro-head. Also contains within the sequence for 19.2 and 19.3 within the gene.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tct...gcg -> agc...gct codon shuffle
sequence annotation: gene 19.2
sequence annotation: gene 19.3
sequence: https://synbiohub.org/public/igem/BBa_Z0137_sequence/1

tct...gcg -> agc...gct codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881265
display id: annotation1881265
version: 1
top level: BBa_Z0137
location: https://synbiohub.org/public/igem/BBa_Z0137/annotation1881265/range1881265/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0137/annotation1881265/range1881265/1
type: Range
persistent identity: range1881265
display id: range1881265
version: 1
top level: BBa_Z0137
direction: direction/forward
start: 4
end: 39
orientation: inline

gene 19.2
type: SequenceAnnotation
persistent identity: annotation1855298
display id: annotation1855298
version: 1
top level: BBa_Z0137
location: https://synbiohub.org/public/igem/BBa_Z0137/annotation1855298/range1855298/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_Z0137/annotation1855298/range1855298/1
type: Range
persistent identity: range1855298
display id: range1855298
version: 1
top level: BBa_Z0137
direction: direction/forward
start: 647
end: 904
orientation: inline

gene 19.3
type: SequenceAnnotation
persistent identity: annotation1855299
display id: annotation1855299
version: 1
top level: BBa_Z0137
location: https://synbiohub.org/public/igem/BBa_Z0137/annotation1855299/range1855299/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_Z0137/annotation1855299/range1855299/1
type: Range
persistent identity: range1855299
display id: range1855299
version: 1
top level: BBa_Z0137
direction: direction/forward
start: 1184
end: 1357
orientation: inline

BBa_Z0155
type: ComponentDefinition
persistent identity: BBa Z0155
display id: BBa_Z0155
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 13
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: Mutations from wild-type on 3' end to mutate Gene 14 RBS
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: g->C mutation
sequence: https://synbiohub.org/public/igem/BBa_Z0155_sequence/1

g->C mutation
type: SequenceAnnotation
persistent identity: annotation1855258
display id: annotation1855258
version: 1
top level: BBa_Z0155
location: https://synbiohub.org/public/igem/BBa_Z0155/annotation1855258/range1855258/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0155/annotation1855258/range1855258/1
type: Range
persistent identity: range1855258
display id: range1855258
version: 1
top level: BBa_Z0155
direction: direction/forward
start: 411
end: 411
orientation: inline

g->C mutation
type: SequenceAnnotation
persistent identity: annotation1855259
display id: annotation1855259
version: 1
top level: BBa_Z0155
location: https://synbiohub.org/public/igem/BBa_Z0155/annotation1855259/range1855259/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0155/annotation1855259/range1855259/1
type: Range
persistent identity: range1855259
display id: range1855259
version: 1
top level: BBa_Z0155
direction: direction/forward
start: 414
end: 414
orientation: inline

BBa_Z0156
type: ComponentDefinition
persistent identity: BBa Z0156
display id: BBa_Z0156
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 14
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Mutations on 3' end to remove Gene 15 RBS
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tgt...ggc->tgc...ggc codon shuffle
sequence annotation: g->C mutation
sequence annotation: added SacII
sequence: https://synbiohub.org/public/igem/BBa_Z0156_sequence/1

tgt...ggc->tgc...ggc codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881264
display id: annotation1881264
version: 1
top level: BBa_Z0156
location: https://synbiohub.org/public/igem/BBa_Z0156/annotation1881264/range1881264/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0156/annotation1881264/range1881264/1
type: Range
persistent identity: range1881264
display id: range1881264
version: 1
top level: BBa_Z0156
direction: direction/forward
start: 4
end: 39
orientation: inline

g->C mutation
type: SequenceAnnotation
persistent identity: annotation1855266
display id: annotation1855266
version: 1
top level: BBa_Z0156
location: https://synbiohub.org/public/igem/BBa_Z0156/annotation1855266/range1855266/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0156/annotation1855266/range1855266/1
type: Range
persistent identity: range1855266
display id: range1855266
version: 1
top level: BBa_Z0156
direction: direction/forward
start: 582
end: 582
orientation: inline

g->C mutation
type: SequenceAnnotation
persistent identity: annotation1855267
display id: annotation1855267
version: 1
top level: BBa_Z0156
location: https://synbiohub.org/public/igem/BBa_Z0156/annotation1855267/range1855267/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_Z0156/annotation1855267/range1855267/1
type: Range
persistent identity: range1855267
display id: range1855267
version: 1
top level: BBa_Z0156
direction: direction/forward
start: 585
end: 585
orientation: inline

added SacII
type: SequenceAnnotation
persistent identity: annotation1884022
display id: annotation1884022
version: 1
top level: BBa_Z0156
location: https://synbiohub.org/public/igem/BBa_Z0156/annotation1884022/range1884022/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_Z0156/annotation1884022/range1884022/1
type: Range
persistent identity: range1884022
display id: range1884022
version: 1
top level: BBa_Z0156
direction: direction/forward
start: 592
end: 597
orientation: inline

BBa_Z0157
type: ComponentDefinition
persistent identity: BBa Z0157
display id: BBa_Z0157
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 17.5
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Mutations on 3' end to remove gene 18 RBS
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: g->A mutation
sequence: https://synbiohub.org/public/igem/BBa_Z0157_sequence/1

g->A mutation
type: SequenceAnnotation
persistent identity: annotation1855282
display id: annotation1855282
version: 1
top level: BBa_Z0157
location: https://synbiohub.org/public/igem/BBa_Z0157/annotation1855282/range1855282/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0157/annotation1855282/range1855282/1
type: Range
persistent identity: range1855282
display id: range1855282
version: 1
top level: BBa_Z0157
direction: direction/forward
start: 198
end: 198
orientation: inline

g->A mutation
type: SequenceAnnotation
persistent identity: annotation1855281
display id: annotation1855281
version: 1
top level: BBa_Z0157
location: https://synbiohub.org/public/igem/BBa_Z0157/annotation1855281/range1855281/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0157/annotation1855281/range1855281/1
type: Range
persistent identity: range1855281
display id: range1855281
version: 1
top level: BBa_Z0157
direction: direction/forward
start: 201
end: 201
orientation: inline

BBa_Z0148
type: ComponentDefinition
persistent identity: BBa Z0148
display id: BBa_Z0148
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 19.5
created: 2006-05-03T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: non-essential; conserved
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_Z0148_sequence/1

BBa_Z0293
type: ComponentDefinition
persistent identity: BBa Z0293
display id: BBa_Z0293
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Right End
created: 2006-05-29T11:00:00Z
modified: 2015-05-08T01:14:55Z
owned by: james
owned by: myers
mutable description: Right End of T7 DNA. Important in packaging. Contains short terminal repeat region and the right terminal repeat. Contains sequence starting after gene 19.5 stop site.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Short Terminal Repeat Region
sequence annotation: Right Terminal Repeat
sequence: https://synbiohub.org/public/igem/BBa_Z0293_sequence/1

Short Terminal Repeat Region
type: SequenceAnnotation
persistent identity: annotation1875189
display id: annotation1875189
version: 1
top level: BBa_Z0293
location: https://synbiohub.org/public/igem/BBa_Z0293/annotation1875189/range1875189/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_Z0293/annotation1875189/range1875189/1
type: Range
persistent identity: range1875189
display id: range1875189
version: 1
top level: BBa_Z0293
direction: direction/forward
start: 68
end: 226
orientation: inline

Right Terminal Repeat
type: SequenceAnnotation
persistent identity: annotation1875190
display id: annotation1875190
version: 1
top level: BBa_Z0293
location: https://synbiohub.org/public/igem/BBa_Z0293/annotation1875190/range1875190/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_Z0293/annotation1875190/range1875190/1
type: Range
persistent identity: range1875190
display id: range1875190
version: 1
top level: BBa_Z0293
direction: direction/forward
start: 240
end: 399
orientation: inline

BBa_Z0144
type: ComponentDefinition
persistent identity: BBa Z0144
display id: BBa_Z0144
version: 1
was generated by: iGEM to SBOL conversion
description: T7.2 gene 18.5 (18.7)
created: 2006-04-26T11:00:00Z
modified: 2015-05-08T01:14:54Z
owned by: james
owned by: myers
mutable description: lysis associated protein. Homologs of 18.5/18.7 are found in all phages (in T1 the two proteins are fused); contains gene 18.7 within coding domain.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sriram Kosuri
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: E promoter remnant
sequence annotation: gaattttta->gagttcctc E-10
sequence annotation: E -10 region
sequence annotation: gene 18.7
sequence annotation: added CATGC to form SphI
sequence: https://synbiohub.org/public/igem/BBa_Z0144_sequence/1

E promoter remnant
type: SequenceAnnotation
persistent identity: annotation1862602
display id: annotation1862602
version: 1
top level: BBa_Z0144
location: https://synbiohub.org/public/igem/BBa_Z0144/annotation1862602/range1862602/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0144/annotation1862602/range1862602/1
type: Range
persistent identity: range1862602
display id: range1862602
version: 1
top level: BBa_Z0144
direction: direction/forward
start: 1
end: 34
orientation: inline

gaattttta->gagttcctc E-10
type: SequenceAnnotation
persistent identity: annotation1862600
display id: annotation1862600
version: 1
top level: BBa_Z0144
location: https://synbiohub.org/public/igem/BBa_Z0144/annotation1862600/range1862600/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_Z0144/annotation1862600/range1862600/1
type: Range
persistent identity: range1862600
display id: range1862600
version: 1
top level: BBa_Z0144
direction: direction/forward
start: 7
end: 15
orientation: inline

E -10 region
type: SequenceAnnotation
persistent identity: annotation1862601
display id: annotation1862601
version: 1
top level: BBa_Z0144
location: https://synbiohub.org/public/igem/BBa_Z0144/annotation1862601/range1862601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_Z0144/annotation1862601/range1862601/1
type: Range
persistent identity: range1862601
display id: range1862601
version: 1
top level: BBa_Z0144
direction: direction/forward
start: 8
end: 13
orientation: inline

gene 18.7
type: SequenceAnnotation
persistent identity: annotation1855761
display id: annotation1855761
version: 1
top level: BBa_Z0144
location: https://synbiohub.org/public/igem/BBa_Z0144/annotation1855761/range1855761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_Z0144/annotation1855761/range1855761/1
type: Range
persistent identity: range1855761
display id: range1855761
version: 1
top level: BBa_Z0144
direction: direction/forward
start: 116
end: 367
orientation: inline

added CATGC to form SphI
type: SequenceAnnotation
persistent identity: annotation1883680
display id: annotation1883680
version: 1
top level: BBa_Z0144
location: https://synbiohub.org/public/igem/BBa_Z0144/annotation1883680/range1883680/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_Z0144/annotation1883680/range1883680/1
type: Range
persistent identity: range1883680
display id: range1883680
version: 1
top level: BBa_Z0144
direction: direction/forward
start: 434
end: 437
orientation: inline

https://synbiohub.org/public/igem/BBa_Z0503_sequence/1
type: Sequence
persistent identity: BBa Z0503 sequence
display id: BBa_Z0503_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccctatctgttacagtctcctaaagtatcctcctaaagtcacctcctaacgtccatcctaaagccaacacctaaagcctacacctaaagacccatcaagtcaacgcctatcttaaagtttaaacataaagaccagacctaaagaccagacctaaagacactacataaagaccagacctaaagacgccttgttgttagccataaagtgataacctttaatcaagcaccgaagtaatacgactcactattagggaagacaacttaaagagacttaaaagattaatttaaaatttatcaaaaagagtattgacttaaagtctaacctataggatacttacagccatcgagagggacacggcgaatagccatcccaatcgacaccggggtcaaccggataagtagacagcctgataagtcgcacgaaaaacaggtattgacaacatgaagtaacatgcagtaagatacaaatcgctaggtaacactagcagcgtcaaccgggcgcacagtgccttctaggtgacttaagcgcaccacggcacataaggtgaaacaaaacggttgacaacatgaagtaaacacggtacgatgtaccacatgaaacgacagtgagtcaccacactgaaaggtgatgcggtctaacgaaacctgacctaagacgctctttaacaatctggtaaatagctcttgagctcgtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcacaatcaataggagaaatcaatatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataaaatcaataggagaaatcaatatggctatgtctaacatgacttacaacaacgttttcgaccacgcttacgaaatgctgaaagaaaacatccgttatgatgacatccgtgacactgatgacctgcacgatgctattcacatggctgccgataatgcagttccgcactactacgctgacatctttagcgtaatggcaagtgagggcattgaccttgagttcgaagactctggtctgatgcctgacaccaaggacgtaatccgcatcctgcaagcgcgtatctatgagcaattaacgattgacctctgggaagacgcagaagacttgctcaatgaatacttggaggaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0252_sequence/1
type: Sequence
persistent identity: BBa Z0252 sequence
display id: BBa_Z0252_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataattaattgaactcactaaagggagaccacagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0106_sequence/1
type: Sequence
persistent identity: BBa Z0106 sequence
display id: BBa_Z0106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacattaccgacatcatgaacgctatcgacgcaatcaaagcactgccaatctgtgaacttgacaagcgtcaaggtatgcttatcgacttactggtcgagatggtcaacagcgagacgtgtgatggcgagctaaccgaactaaatcaggcacttgagcatcaagattggtggactaccttgaagtgtctcacggctgacgcagggttcaagatgctcggtaatggtcacttctcggctgcttatagtcacccgctgctacctaacagagtgattaaggtgggctttaagaaagaggattcaggcgcagcctataccgcattctgccgcatgtatcagggtcgtcctggtatccctaacgtctacgatgtacagcgccacgctggatgctatacggtggtacttgacgcacttaaggattgcgagcgtttcaacaatgatgcccattataaatacgctgagattgcaagcgacatcattgattgcaattcggatgagcatgatgagttaactggatgggatggtgagtttgttgaaacttgtaaactaatccgcaagttctttgagggcatcgcctcattcgacatgcatagcgggaacatcatgttctcaaatggagacgtaccatacatcaccgacccggtatcattctcgcagaagaaagacggtggcgcattcagcatcgaccctgaggaactcatcaaggaagtcgaggaagtcgcacgacagaaagaaattgaccgcgctaaggcccgtaaagaacgtcacgaggggcgcttagaggcacgcagattcaaacgtcgcaaccgcaaggcacgtaaagcacacaaagctaagcgcgaaagaatgcttgctgcgtggcgatgggctgaacgtcaagaacggcgtaaccatgaggtagctgtagatgtactaggaagaaccaataacgctatgctctgggtcaacatgttctctggggactttaaggcgcttgaggaacgaatcgcgctgcactggcgtaatgctgaccggatggctatcgctaatggtcttacgctcaacattgataagcaacttgacgcaatgttaatgggctgacgtacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0107_sequence/1
type: Sequence
persistent identity: BBa Z0107 sequence
display id: BBa_Z0107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0108_sequence/1
type: Sequence
persistent identity: BBa Z0108 sequence
display id: BBa_Z0108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaacttcgaaaagatgaccaaacgttctaaccgtaatgctcgtgacttcgaggcaaccaaaggtcgcaagttgaataagactaagcgtgaccgctctcacaagcgtagctgggagggtcagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0115_sequence/1
type: Sequence
persistent identity: BBa Z0115 sequence
display id: BBa_Z0115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggacgtttatatagtggtaatctggcagcattcaaggcagcaacaaacaagctgttccagttagacttagcggtcatttatgatgactggtatgatgcctatacaagaaaagattgcatacggttacgtattgaggacaggagtggaaacctgattgatactagcaccttctaccaccacgacgaggacgttctgttcaatatgtgtactgattggttgaaccatatgtatgaccagttgaaggactggaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0151_sequence/1
type: Sequence
persistent identity: BBa Z0151 sequence
display id: BBa_Z0151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggactgttagatggtgaagcctgggaaaaagaaaacccgccagtacaagcaactgggtgtatagcttgcttagagaaagatgaccgttatccacacacctgtaacaaaggagctaacgatatgaccgaacgtgaacaagagatgatcattaagttgatagacaataatgaaggtcgcccagatgatttgaatggctgcggtattctctgctccaatgtcccttgccacctctgccccgcaaataacgatcaaaagataaccttaggtgaaatccgagcgatggacccacgtaaaccacatctgaataaacctgaggtaactcctacagatgaccagccttccgctgagacaatcgaaggtgtcactaagccttcccactacatgctgtttgacgacattgaggctatcgaagtgattgctcgttcaatgaccgttgagcagttcaagggatactgcttcggtaacatcttaaagtacagactacgtgctggtaagaagtcagagttagcgtacttagagaaagacctagcgaaagcagacttctataaagaactctttgagaaacataaagataaatgttacgcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0158_sequence/1
type: Sequence
persistent identity: BBa Z0158 sequence
display id: BBa_Z0158_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctatgtctaacatgacttacaacaacgttttcgaccacgcttacgaaatgctgaaagaaaacatccgttatgatgacatccgtgacactgatgacctgcacgatgctattcacatggctgccgataatgcagttccgcactactacgctgacatctttagcgtaatggcaagtgagggcattgaccttgagttcgaagactctggtctgatgcctgacaccaaggacgtaatccgcatcctgcaagcgcgtatctatgagcaattaacgattgacctctgggaagacgcagaagacttgctcaatgaatacttggaggaagtcgaggagtacgaggaggacgaagagtaagggccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0253_sequence/1
type: Sequence
persistent identity: BBa Z0253 sequence
display id: BBa_Z0253_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcaccgaagtaatacgactcactattagggaaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0261_sequence/1
type: Sequence
persistent identity: BBa Z0261 sequence
display id: BBa_Z0261_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatcaataggagaaatcaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0262_sequence/1
type: Sequence
persistent identity: BBa Z0262 sequence
display id: BBa_Z0262_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaagaggagaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0271_sequence/1
type: Sequence
persistent identity: BBa Z0271 sequence
display id: BBa_Z0271_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacactggctcaccttcgggtgggcctttctgcgttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0291_sequence/1
type: Sequence
persistent identity: BBa Z0291 sequence
display id: BBa_Z0291_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacgccaaatcaatacgactcactatagagggacaaactcaaggtcattcgcaagagtggcctttatgattgaccttcttccggttaatacgactcactataggagaaccttaaggtttaactttaagacccttaagtgttaattagagatttaaatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0292_sequence/1
type: Sequence
persistent identity: BBa Z0292 sequence
display id: BBa_Z0292_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccctatctgttacagtctcctaaagtatcctcctaaagtcacctcctaacgtccatcctaaagccaacacctaaagcctacacctaaagacccatcaagtcaacgcctatcttaaagtttaaacataaagaccagacctaaagaccagacctaaagacactacataaagaccagacctaaagacgccttgttgttagccataaagtgataacctttaatca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0294_sequence/1
type: Sequence
persistent identity: BBa Z0294 sequence
display id: BBa_Z0294_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caacttaaagagacttaaaagattaatttaaaatttatcaaaaagagtattgacttaaagtctaacctataggatacttacagccatcgagagggacacggcgaatagccatcccaatcgacaccggggtcaaccggataagtagacagcctgataagtcgcacgaaaaacaggtattgacaacatgaagtaacatgcagtaagatacaaatcgctaggtaacactagcagcgtcaaccgggcgcacagtgccttctaggtgacttaagcgcaccacggcacataaggtgaaacaaaacggttgacaacatgaagtaaacacggtacgatgtaccacatgaaacgacagtgagtcaccacactgaaaggtgatgcggtctaacgaaacctgacctaagacgctctttaacaatctggtaaatagctcttgagctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0273_sequence/1
type: Sequence
persistent identity: BBa Z0273 sequence
display id: BBa_Z0273_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0251_sequence/1
type: Sequence
persistent identity: BBa Z0251 sequence
display id: BBa_Z0251_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaattaatacgactcactatagggagaccacaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0109_sequence/1
type: Sequence
persistent identity: BBa Z0109 sequence
display id: BBa_Z0109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgaacattaagactaacccgtttaaagccgtgtctttcgtagagtctgccattaagaaggctctggataacgctgggtatcttatcgctgaaatcaagtacgatggtgtacgcgggaacatctgcgtagacaatactgctaacagttactggctctctcgtgtatctaaaacgattccggcactggagcacttaaacgggtttgatgttcgctggaagcgtctactgaacgatgaccgttgcttctacaaagatggctttatgcttgatggggaactcatggtcaagggcgtagactttaacacagggtccggcctactgcgtaccaaatggactgacacgaagaaccaagagttccatgaagagttattcgttgaaccaatccgtaagaaagataaagttccctttaagctgcacactggacaccttcacataaaactgtacgctatcctcccgctgcacatcgtggagtctggagaagactgtgatgtcatgacgttgctcatgcaggaacacgttaagaacatgctgcctctgctacaggaatacttccctgaaatcgaatggcaagcggctgaatcttacgaggtctacgatatggtagaactacagcaactgtacgagcagaagcgagcagaaggccatgagggtctcattgtgaaagacccgatgtgtatctataagcgcggtaagaaatctggctggtggaaaatgaaacctgagaacgaagctgacggtatcattcagggtctggtatggggtacaaaaggtctggctaatgaaggtaaagtgattggttttgaggtgcttcttgagagtggtcgtttagttaacgccacgaatatctctcgcgccttaatggatgagttcactgagacagtaaaagaggccaccctaagtcaatggggattctttagcccatacggtattggcgacaacgatgcttgtactattaacccttacgatggctgggcgtgtcaaattagctacatggaggaaacacctgatggctctttgcggcacccatcgttcgtaatgttccgtggcaccgaggacaaccctcaagagaaaatgtaacggccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0116_sequence/1
type: Sequence
persistent identity: BBa Z0116 sequence
display id: BBa_Z0116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaatgtaaataccggatccttaagcgtagataataagaagttttgggctaccgtagagtcctcggagcattccttcgaggttccaatctacgctgagaccctagacgaagctctggagttagccgaatggcaatacgttccggctggctttgaggttactcgtgtgcgtccttgtgtagcaccgaagtaagaattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0118_sequence/1
type: Sequence
persistent identity: BBa Z0118 sequence
display id: BBa_Z0118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaggttacggcgctaaaggaatccgaaaggttggagcgtttcgctctggcctagaggacaaggtttcaaagcagttggaatcaaaaggtattaaattcgagtatgaagagtggaaagtgccttatgtaattccggcgagcaatcacacttacactccagacttcttacttccaaacggtatattcgttgagacaaagggtctgtgggaaagcgatgatagaaagaagcacttattaattagggagcagcaccccgagctagacatccgtattgtcttctcaagctcacgtactaagttatacaaaggttctccaacgtcttatggagagttctgcgaaaagcatggtattaagttcgctgataaactgatacctgctgagtggataaaggaacccaagaaggaggtcccctttgatagattaaaaaggaaaggaggaaagaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0119_sequence/1
type: Sequence
persistent identity: BBa Z0119 sequence
display id: BBa_Z0119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctcgtgtacagtttaaacaacgtgaatctactgacgcaatctttgttcactgctcggctaccaagccaagtcagaatgttggtgtccgtgagattcgccagtggcacaaagagcagggctggctcgatgtgggataccacttcatcatcaagcgagacggtactgtggaggcaggacgagatgagatggctgtaggctctcacgctaagggttacaaccacaactctatcggcgtctgccttgttggtggtatcgacgataaaggtaagttcgacgctaactttacgccagcccaaatgcaatcccttcgctcactgcttgtcacactgctggctaagtacgaaggcgctgtgcttcgcgcccatcatgaggtggcgccgaaggcttgcccttcgttcgaccttaagcgttggtgggagaagaacgaactggtcacttctgaccgtggataagatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0301_sequence/1
type: Sequence
persistent identity: BBa Z0301 sequence
display id: BBa_Z0301_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0120_sequence/1
type: Sequence
persistent identity: BBa Z0120 sequence
display id: BBa_Z0120_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggataatagtcatgatagcgatagcgtttttttgtatcacattccttgtgacaactgtgggagtagtgacgggaactcgctgttctctgacggacacacgttctgctacgtatgcgagaagtggactgctggtaatgaagacactaaagagagggcttcaaaacggaaaccctcaggaggtaaaccaatgacttacaacgtgtggaacttcggggaatccaatggacgctactccgcgttaactgcgagaggaatctccaaggaaacctgtcagaaggctggctactggattgccaaagtagacggtgtgatgtaccaagtggctgactatcgggaccagaacggcaacattgtgagtcagaaggttcgagataaagataagaactttaagaccactggtagtcacaagagtgacgctctgttcgggaagcacttgtggaatggtggtaagaagattgtcgttacagaaggtgaaatcgacatgcttaccgtgatggaacttcaagactgtaagtatcctgtagtgtcgttgggtcacggtgcctctgccgctaagaagacatgcgctgccaactacgaatacttcgatcagttcgaacagattatcctgatgttcgatatggacgaagcagggcgcaaagcagtcgaagaggctgcacaggttctacctgctggtaaggtacgagtggcagttcttccgtgtaaggatgcaaacgagtgtcacctaaatggtcacgaccgtgaaatcatggagcaagtgtggaatgctggtccttggattcctgatggtgtggtatcggctctttcgttacgtgaacgaatccgtgagcacctatcgtccgaggaatcagtaggtttacttttcagtggctgcactggtatcaacgataagaccttaggtgcccgtggtggtgaagtcattatggtcacttccggttccggtatgggtaagtcaacgttcgtccgtcaacaagctctacaatggggcacagcgatgggcaagaaggtaggcttagcgatgcttgaggagtccgttgaggagaccgctgaggaccttataggtctacacaaccgtgttcgtctgcgtcagtctgactctctgaaacgtgaaatcattgagaacggtaagttcgaccaatggttcgatgaactgttcggcaacgatacgttccatctatatgactcattcgccgaggctgagacggatagactgctcgctaagctggcctacatgcgctcaggcttgggctgtgacgtaatcattctagaccacatctcaatcgtcgtatccgcttctggtgaatccgatgagcgtaagatgattgacaacctgatgaccaagctcaaagggttcgctaagtcaactggggtggtgctggtcgtaatttgtcaccttaagaacccagacaaaggtaaagcacacgaggaaggtcgccccgtttctattactgacctacgtggttctggcgcactacgccaactatctgatactattattgcccttgagcgtaatcagcaaggcgatatgcctaaccttgtcctcgttcgtattctcaagtgccgctttactggtgatactggtatcgctggctacatggaatacaacaaggaaaccggatggcttgaaccatcaagttactcaggggaagaagagtcacactcagagtcaacagactggtccaacgacactgacttctaagccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0121_sequence/1
type: Sequence
persistent identity: BBa Z0121 sequence
display id: BBa_Z0121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgtaagtgatatagaagcgaatgcattattagaaagcgtcactaagttccactgcggggttatctacgactactccaccgctgagtacgtaagctaccgtccgagtgacttcggtgcgtatctggatgcgctggaagccgaggttgcacgaggcggtcttattgtgttccacaacggtcacaagtatgacgttcctgcattgaccaaactggcaaagttgcaattgaaccgagagttccaccttcctcgtgagaactgtattgacacccttgtgttgtcacgtttgattcattccaacctcaaggacaccgatatgggtcttctgcgttccggcaagttgcccggaaaacgctttgggtctcacgctttggaggcgtggggttatcgcttaggcgagatgaagggtgaatacaaagacgactttaagcgtatgcttgaagagcagggtgaagaatacgttgacggaatggagtggtggaacttcaacgaagagatgatggactataacgttcaggacgttgtggtaactaaagctctccttgagaagctactctctgacaaacattacttccctcctgagattgactttacggacgtaggatacactacgttctggtcagaatcccttgaggccgttgacattgaacatcgtgctgcatggctgctcgctaaacaagagcgcaacgggttcccgtttgacacaaaagcaatcgaagagttgtacgtagagttagctgctcgccgctctgagttgctccgtaaattgaccgaaacgttcggctcgtggtatcagcctaaaggtggcactgagatgttctgccatccgcgaacaggtaagccactacctaaataccctcgcattaagacacctaaagttggtggtatctttaagaagcctaagaacaaggcacagcgagaaggccgtgagccttgcgaacttgatacccgcgagtacgttgctggtgctccttacaccccagttgaacatgttgtgtttaacccttcgtctcgtgaccacattcagaagaaactccaagaggctgggtgggtcccgaccaagtacaccgataagggtgctcctgtggtggacgatgaggtactcgaaggagtacgtgtagatgaccctgagaagcaagccgctatcgacctcattaaagagtacttgatgattcagaagcgaatcggacagtctgctgagggagacaaagcatggcttcgttatgttgctgaggatggtaagattcatggttctgttaaccctaatggagcagttacgggtcgtgcgacccatgcgttcccaaaccttgcgcaaattccgggtgtacgttctccttatggagagcagtgtcgcgctgcttttggcgctgagcaccatttggatgggataactggtaagccttgggttcaggctggcatcgacgcatccggtcttgagctacgctgcttggctcacttcatggctcgctttgataacggcgagtacgctcacgagattcttaacggcgacatccacactaagaaccagatagctgctgaactacctacccgagataacgctaagacgttcatctatgggttcctctatggtgctggtgatgagaagattggacagattgttggtgctggtaaagagcgcggtaaggaactcaagaagaaattccttgagaacacccccgcgattgcagcactccgcgagtctatccaacagacacttgtcgagtcctctcaatgggtagctggtgagcaacaagtcaagtggaaacgccgctggattaaaggtctggatggtcgtaaggtacacgttcgtagtcctcacgctgccttgaataccctactgcaatctgctggtgctctcatctgcaaactgtggattatcaagaccgaagagatgctcgtagagaaaggcttgaagcatggctgggatggggactttgcgtacatggcatgggtacatgatgaaatccaagtaggctgccgtaccgaagagattgctca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0123_sequence/1
type: Sequence
persistent identity: BBa Z0123 sequence
display id: BBa_Z0123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgtttctcaccgaaaattaaaactccgaagatggataccaatcagattcgagccgttgagccagcgcctctgacccaagaagtgtcaagcgtggagttcggtgggtcttctgatgagacggataccgagggcaccgaagtgtctggacgcaaaggcctcaaggtcgaacgtgatgattccgtagcgaagtctaaagccagcggcaatggctccgctcgtatgaaatcttccatccgtaagtccgcatttggaggtaagaagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0124_sequence/1
type: Sequence
persistent identity: BBa Z0124 sequence
display id: BBa_Z0124_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggtaagaaagttaagaaggccgtgaagaaagtcaccaagtccgttaagaaagtcgttaaggaaggggctcgtccggttaaacaggttgctggcggtctagctggtctggctggtggtactggtgaagcacagatggtggaagtaccacaagctgccgcacagattgttgacgtacctgagaaagaggtttccactgaggacgaagcacagacagaaagcggacgcaagaaagctcgtgctggcggtaagaaatccttgagtgtagcccgtagctccggtggcggtatcaacatctaaggtcacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0125_sequence/1
type: Sequence
persistent identity: BBa Z0125 sequence
display id: BBa_Z0125_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgagaaacgaacaggacttgcggaggatggcgcaaagtctgtctatgagcgtttaaagaacgaccgtgctccctatgagacacgcgctcagaattgcgctcaatataccatcccatcattgttccctaaggactccgataacgcctctacagattatcaaactccgtggcaagccgtgggcgctcgtggtctgaacaatctagcctctaagctcatgctggctctattccctatgcagacttggatgcgacttactatatctgaatatgaagcaaagcagttactgagcgaccccgatggactcgctaaggtcgatgagggcctctcgatggtagagcgtatcatcatgaactacattgagtctaacagttaccgcgtgactctctttgaggctctcaaacagttagtcgtagctggtaacgtcctgctgtacctaccggaaccggaagggtcaaactataatcccatgaagctgtaccgattgtcttcttatgtggtccaacgagacgcattcggcaacgttctgcaaatggtgactcgtgaccagatagcttttggtgctctccctgaggacatccgtaaggctgtagaaggtcaaggtggtgagaagaaagctgatgagacaatcgacgtgtacactcacatctatctggatgaggactcaggtgaatacctccgatacgaagaggtcgagggtatggaagtccaaggctccgatgggacttatcctaaagaggcttgcccatacatcccgattcggatggtcagactagatggtgaatcctacggtcgttcgtacattgaggaatacttaggtgacttacggtcccttgaaaatctccaagaggctatcgtcaagatgtccatgattagctctaaggttatcggcttagtgaatcctgctggtatcacccagccacgccgactgaccaaagctcagactggtgacttcgttactggtcgtccagaagacatctcgttcctccaactggagaagcaagcagactttactgtagctaaagccgtaagtgacgctatcgaggctcgcctttcgtttgcctttatgttgaactctgcggttcagcgtacaggtgaacgtgtgaccgccgaagagattcggtatgtagcttctgaacttgaagatactttaggtggtgtctactctatcctttctcaagaattacaattgcctctggtacgagtgctcttgaagcaactacaagccacgcaacagattcctgagttacctaaggaagccgtagagccaaccattagtacaggtctggaagcaattggtcgaggacaagaccttgataagctggagcggtgtgtcactgcgtgggctgcactggcacctatgcgggacgaccctgatattaaccttgcgatgattaagttacgtattgccaacgctatcggtattgacacttctggtattctactcaccgaagaacagaagcaacagaagatggcccaacagtctatgcaaatgggtatggataatggtgctgctgcgctggctcaaggtatggctgcacaagctacagcttcacctgaggctatggctgctgccgctgattccgtaggtttacagccgggaatttaagtcgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0126_sequence/1
type: Sequence
persistent identity: BBa Z0126 sequence
display id: BBa_Z0126_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcagagagtaacgcagatgtatacgcaagttttggcgtgaactccgctgtgatgtctggtggttccgttgaggaacatgagcagaacatgctggctcttgatgttgctgcccgtgatggcgatgatgcaatcgagttagcgtcagacgaagtggaaacagaacgtgacctgtatgacaactctgacccgttcggtcaagaggatgacgaaggccgcattcaggttcgtatcggtgatggctctgagccgaccgatgtggacactggagaagaaggcgttgagggcaccgaaggttccgaagagtttaccccactgggcgagactccagaagaactggtagctgcctctgagcaacttggtgagcacgaagagggcttccaagagatgattaacattgctgctgagcgtggcatgagtgtcgagaccattgaggctatccagcgtgagtacgaggagaacgaagagttgtccgccgagtcctacgctaagctggctgaaattggctacacgaaggctttcattgactcgtatatccgtggtcaagaagctctggtggagcagtacgtaaacagtgtcattgagtacgctggtggtcgtgaacgttttgatgcactgtataaccaccttgagacgcacaaccctgaggctgcacagtcgctggataatgcgttgaccaatcgtgacttagcgaccgttaaggctatcatcaacttggctggtgagtctcgcgctaaggcgttcggtcgtaagccaactcgtagtgtgactaatcgtgctattccggctaaacctcaggctaccaagcgtgaaggctttgcggaccgtagcgagatgattaaagctatgagtgaccctcggtatcgcacagatgccaactatcgtcgtcaagtcgaacagaaagtaatcgattcgaacttctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0127_sequence/1
type: Sequence
persistent identity: BBa Z0127 sequence
display id: BBa_Z0127_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagcatgactggtggacagcaaatgggtactaaccaaggtaaaggtgtagttgctgctggagataaactggcgttgttcttgaaggtatttggcggtgaagtcctgactgcgttcgctcgtacctccgtgaccacttctcgccacatggtacgttccatctccagcggtaaatccgctcagttccctgttctgggtcgcactcaggcagcgtatctggctccgggcgagaacctcgacgataaacgtaaggacatcaaacacaccgagaaggtaatcaccattgacggtctcctgacggctgacgttctgatttatgatattgaggacgcgatgaaccactacgacgttcgctctgagtatacctctcagttgggtgaatctctggcgatggctgcggatggtgcggttctggctgagattgccggtctgtgtaacgtggaaagcaaatataatgagaacatcgagggcttaggtactgctaccgtaattgagaccactcagaacaaggccgcacttaccgaccaagttgcgctgggtaaggagattattgcggctctgactaaggctcgtgcggctctgaccaagaactatgttccggctgctgaccgtgtgttctactgtgacccagatagctactctgcgattctggcagcactgatgccgaacgcagcaaactacgctgctctgattgaccctgagaagggttctatccgcaacgttatgggctttgaggttgtagaagttccgcacctcaccgctggtggtgctggtaccgctcgtgagggcactactggtcagaagcacgtcttccctgccaataaaggtgagggtaatgtcaaggttgctaaggacaacgttatcggcctgttcatgcaccgctctgcggtaggtactgttaagctgcgtgacttggctctggagcgcgctcgccgtgctaacttccaagcggaccagattatcgctaagtacgcaatgggccacggtggtcttcgcccagaagctgctggtgcagtggttttcaaagtggagtaatgctgggggtggcctcaacggtcgctgctagtcccgaagaggcgagtgttacttcaacagaagaaaccttaacgccagcacaggaggccgcacgcacccgcgctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataacccggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0128_sequence/1
type: Sequence
persistent identity: BBa Z0128 sequence
display id: BBa_Z0128_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgaagttatgatatgaatgtagaaacagcggcagaattatcagctgtgaacgacattctggcgtctatcggtgaacctccggtatcaacgctggaaggtgacgctaacgcagatgcagcgaacgctcggcgtattctcaacaagattaaccgacagattcaatctcgtggatggacgttcaacattgaggaaggcataacgctactacctgatgtttactccaacctgattgtatacagtgacgactatttatccctaatgtctacttccggtcaatccatctacgttaaccgaggtggctatgtgtatgaccgaacgagtcaatcagaccgctttgactctggtattactgtgaacattattcgtctccgcgactacgatgagatgcctgagtgcttccgttactggattgtcaccaaggcttcccgtcagttcaacaaccgattctttggggcaccggaagtagagggtgtactccaagaagaggaagatgaggctagacgtctctgcatggagtatgagatggactacggtgggtacaatatgctggatggagatgcgttcacttctggtctactgactcgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0138_sequence/1
type: Sequence
persistent identity: BBa Z0138 sequence
display id: BBa_Z0138_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcaaactgattaagaagttaggccaactgctggttcgtatgtacaacgtggaagccaagcgactgaacgatgaggctcgtaaagaggccacacagtcacgcgctctggcgattcgctccaacgaactggctgacagtgcatccactaaagttaccgaggctgcccgtgtggcaaaccaagctcaacagctttccaaattctttgagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0139_sequence/1
type: Sequence
persistent identity: BBa Z0139 sequence
display id: BBa_Z0139_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctaacgtagctgaaactatccgtctatccgatacagctgaccagtggaaccgtcgagtccacatcaacgttcgcaacggtaaggcgactatggtttaccgctggaaggactctaagtcctctaagaatcacactcagcgtatgacgttgacagatgagcaagcactgcgtctggtcaatgcgcttaccaaagctgccgtgacagcaattcatgaagctggtcgcgtcaatgaagctatggctatcctcgacaagattgataactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0152_sequence/1
type: Sequence
persistent identity: BBa Z0152 sequence
display id: BBa_Z0152_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaaaaaaatatttacaagtgcgttaggaacagctgaaccttacgcttacatcgccaagccggactacggcaacgaagagcgtggctttgggaaccctcgtggtgtctataaagttgacctgactattcccaacaaagacccgcgctgccagcgtatggtcgatgaaatcgtgaagtgtcacgaagaggcttatgctgctgccgttgaggaatacgaagctaatccacctgctgtagctcgtggtaagaaaccgctgaaaccgtatgagggtgacatgccgttcttcgataacggtgacggtacgactacctttaagttcaaatgctacgcgtctttccaagacaagaagaccaaagagaccaagcacatcaatctggttgtggttgactcaaaaggtaagaagatggaagacgttccgattatcggtggtggctctaagctgaaagttaaatattctctggttccatacaagtggaacactgctgtaggtgcgagcgttaagctgcaactggaatccgtgatgctggtcgaactggctacctttggtggcggtgaagacgattgggctgacgaagttgaagagaacggctatgttgcctctggttctgccaaagcgagcaaaccacgcgacgaagaaagctgggacgaagacgacgaagagtccgaggaagcagacgaagacggggacttctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0129_sequence/1
type: Sequence
persistent identity: BBa Z0129 sequence
display id: BBa_Z0129_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcactgatatcccaaagcattaaaaatttaaaaggcggtatcagccaacagcctgacatccttcgttatccagaccaagggtcacgccaagttaacggttggtcttcggagaccgagggcctccaaaagcgtccacctcttgttttcttaaatacacttggagacaacggtgcgttaggtcaagctccgtacatccacctgattaaccgagatgagcacgaacagtattacgctgtgttcactggtagcggaatccgagtgttcgacctttctggtaacgagaagcaagttaggtatcctaacggttccaactacatcaagaccgctaatccacgtaacgacctgcgaatggttactgtagcagactatacgttcatcgtgaaccgtaacgttgttgcacagaagaacacaaagtctgtcaacttaccgaattacaaccctaatcaagacggattgattaacgttcgtggtggtcagtatggtagggaactaattgtacacattaacggtaaagacgttgcgaagtataagataccagatggtagtcaacctgaacacgtaaacaatacggatgcccaatggttagctgaagagttagccaagcagatgcgcactaacttgtctgattggactgtaaatgtagggcaagggttcatccatgtgaccgcacctagtggtcaacagattgactccttcacgactaaagatggctacgcagaccagttgattaaccctgtgacccactacgctcagtcgttctctaagctgccacctaatgctcctaacggctacatggtgaaaatcgtaggggacgcctctaagtctgccgaccagtattacgttcggtatgacgctgagcggaaagtttggactgagactttaggttggaacactgaggaccaagttctatgggaaaccatgccacacgctcttgtgcgagccgctgacggtaatttcgacttcaagtggcttgagtggtctcctaagtcttgtggtgacgttgacaccaacccttggccttcttttgttggttcaagtattaacgatgtgttcttcttccgtaaccgcttaggattccttagtggggagaacatcatattgagtcgtacagccaaatacttcaacttctaccctgcgtccattgcgaaccttagtgatgacgaccctatagacgtagctgtgagtaccaaccgaatagcaatccttaagtacgccgttccgttctcagaagagttactcatctggtccgatgaagcacaattcgtcctgactgcctcgggtactctcacatctaagtcggttgagttgaacctaacgacccagtttgacgtacaggaccgagcgagaccttttgggattgggcgtaatgtctactttgctagtccgaggtccagcttcacgtccatccacaggtactacgctgtgcaggatgtcagttccgttaagaatgctgaggacattacatcacacgttcctaactacatccctaatggtgtgttcagtatttgcggaagtggtacggaaaacttctgttcggtactatctcacggggaccctagtaaaatcttcatgtacaaattcctgtacctgaacgaagagttaaggcaacagtcgtggtctcattgggactttggggaaaacgtacaggttctagcttgtcagagtatcagctcagatatgtatgtgattcttcgcaatgagttcaatacgttcctagctagaatctctttcactaagaacgccattgacttacagggagaaccctatcgtgcctttatggacatgaagattcgatacacgattcctagtggaacatacaacgatgacacattcactacctctattcatattccaacaatttatggtgcaaacttcgggaggggcaaaatcactgtattggagcctgatggtaagataaccgtgtttgagcaacctacggctgggtggaatagcgacccttggctgagactcagcggtaacttggagggacgcatggt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0132_sequence/1
type: Sequence
persistent identity: BBa Z0132 sequence
display id: BBa_Z0132_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtaaaattgaatctgcccttcaagcggcacaaccgggactctctcggttacgtggtggtgctggaggtatgggctatcgtgcagcaaccactcaggccgaacagccaaggtcaagcctattggacaccattggtcggttcgctaaggctggtgccgatatgtataccgctaaggaacaacgagcacgagacctagctgatgaacgctctaacgagattatccgtaagctgacccctgagcaacgtcgagaagctctcaacaacgggacccttctgtatcaggatgacccatacgctatggaagcactccgagtcaagactggtcgtaacgctgcgtatcttgtggacgatgacgttatgcagaagataaaagagggtgtcttccgtactcgcgaagagatggaagagtatcgccatagtcgccttcaagagggcgctaaggtatacgctgagcagttcggcatcgaccctgaggacgttgattatcagcgtggtttcaacggggacattaccgagcgtaacatctcgctgtatggtgcgcatgataacttcttgagccagcaagctcagaagggcgctatcatgaacagccgagtggaactcaacggtgtccttcaagaccctgatatgctgcgtcgtccagactctgctgacttctttgagaagtatatcgacaacggtctggttactggcgcaatcccatctgatgctcaagccacacagcttataagccaagcgttcagtgacgcttctagccgtgctggtggtgctgacttcctgatgcgagtcggtgacaagaaggtaacacttaacggagccactacgacttaccgagagttgattggtgaggaacagtggaacgctctcatggtcacagcacaacgttctcagtttgagactgacgcgaagctgaacgagcagtatcgcttgaagattaactctgcgctgaaccaagaggacccaaggacagcttgggagatgcttcaaggtatcaaggctgaactagataaggtccaacctgatgagcagatgacaccacaacgtgagtggctaatctccgcacaggaacaagttcagaatcagatgaacgcatggacgaaagctcaggccaaggctctggacgattccatgaagtcaatgaacaaacttgacgtaatcgacaagcaattccagaagcgaatcaacggtgagtgggtctcaacggattttaaggatatgccagtcaacgagaacactggtgagttcaagcatagcgatatggttaactacgccaataagaagctcgctgagattgacagtatggacattccagacggtgccaaggatgctatgaagttgaagtaccttcaagcggactctaaggacggagcattccgtacagccatcggaaccatggtcactgacgctggtcaagagtggtctgccgctgtgattaacggtaagttaccagaacgaaccccagctatggatgctctgcgcagaatccgcaatgctgaccctcagttgattgctgcgctatacccagaccaagctgagctattcctgacgatggacatgatggacaagcagggtattgaccctcaggttattcttgatgccgaccgactgactgttaagcggtccaaagagcaacgctttgaggatgataaagcattcgagtctgcactgaatgcatctaaggctcctgagattgcccgtatgccagcgtcactgcgcgaatctgcacgtaagatttatgactccgttaagtatcgctcggggaacgaaagcatggctatggagcagatgaccaagttccttaaggaatctacctacacgttcactggtgatgatgttgacggtgataccgttggtgtgattcctaagaatatgatgcaggttaactctgacccgaaatcatgggagcaaggtcgggatattctggaggaagcacgtaagggaatcattgcgagcaacccttggataaccaataagcaactgaccatgtattctcaagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0154_sequence/1
type: Sequence
persistent identity: BBa Z0154 sequence
display id: BBa_Z0154_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggaaatttacctctggagaccgtagctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0159_sequence/1
type: Sequence
persistent identity: BBa Z0159 sequence
display id: BBa_Z0159_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttaacacctattaaccaattacttaagaaccctaacgatattccagatgtacctcgtgcaaccgctgagtatctacaggttcgattcaactatgcgtacctcgaagcgtctggtcatataggacttatgcgtgctaatggttgtagtgaggcccacatcttgggtttcattcagggcctacagtatgcctctaacgtcattgacgagattgagttacgcaaggaacaactaagagatgatggcgaagattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0160_sequence/1
type: Sequence
persistent identity: BBa Z0160 sequence
display id: BBa_Z0160_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctatgacaaagaaatttaaagtgtccttcgacgttaccgcaaagatgtcgtctgacgttcaggcaatcttagagaaagatatgctgcatctatgtaagcaggtcggctcaggtgcgattgtccccaatggtaaacagaaggaaatgattgtccagttcctgacacacggtatggaaggattgatgacattcgtagtacgtacatcatttcgtgaggccattaaggacatgcacgaagagtatgcagataaggactctttcaaacaatctcctgcaacagtacgggaggtgttctgatgtctgactacctgaaagtgctgcaagcaatcaaaagttgccctaagactttccagtccaactatgtacggaacaatgcgagcctcgtagcggaggccgcttcccgtggtcacatctcgtgcctgactactagtggacgtaacggtggcgcttgggaaatcactgcttccggtactcgctttctgaaacgaatgggcgggtgtgtctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0272_sequence/1
type: Sequence
persistent identity: BBa Z0272 sequence
display id: BBa_Z0272_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0133_sequence/1
type: Sequence
persistent identity: BBa Z0133 sequence
display id: BBa_Z0133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggataagtacgataagaacgtaccaagtgattatgatggtctgttccaaaaggctgctgatgccaacggggtctcttatgaccttttacgtaaagtcgcttggacagaatcacgatttgtgcctacagcaaaatctaagactggaccattaggcatgatgcaatttaccaaggcaaccgctaaggccctcggtctgcgagttaccgatggtccagacgacgaccgactgaaccctgagttagctattaatgctgccgctaagcaacttgcaggtctggtagggaagtttgatggcgatgaactcaaagctgcccttgcgtacaaccaaggcgagggacgcttgggtaatccacaacttgaggcgtactctaagggagacttcgcatcaatctctgaggagggacgtaactacatgcgtaaccttctggatgttgctaagtcacctatggctggacagttggaaacttttggtggcataaccccaaagggtaaaggcattccggctgaggtaggattggctggaattggtcacaagcagaaagtaacacaggaacttcctgagtccacaagttttgacgttaagggtatcgaacaggaggctacggcgaaaccattcgccaaggacttttgggagacccacggagaaacacttgacgagtacaacagtcgttcaaccttcttcggattcaaaaatgctgccgaagctgaactctccaactcagtcgctgggatggctttccgtgctggtcgtctcgataatggttttgatgtgtttaaagacaccattacgccgactcgctggaactctcacatctggactccagaggagttagagaagattcgaacagaggttaagaaccctgcgtacatcaacgttgtaactggtggttcccctgagaacctcgatgacctcattaaattggctaacgagaactttgagaatgactcccgcgctgccgaggctggcctaggtgccaaactgagtgctggtattattggtgctggtgtggacccgcttagctatgttcctatggtcggtgtcactggtaagggctttaagttaatcaataaggctcttgtagttggtgccgaaagtgctgctctgaacgttgcatccgaaggtctccgtacctccgtagctggtggtgacgcagactatgcgggtgctgccttaggtggctttgtgtttggcgcaggcatgtctgcaatcagtgacgctgtagctgctggactgaaacgcagtaaaccagaagctgagttcgacaatgagttcatcggtcctatgatgcgattggaagcccgtgagacagcacgaaacgccaactctgcggacctctctcggatgaacactgagaacatgaagtttgaaggtgaacataatggtgtcccttatgaggacttaccaacagagagaggtgccgtggtgttacatgatggctccgttctaagtgcaagcaacccaatcaaccctaagactctaaaagagttctccgaggttgaccctgagaaggctgcgcgaggaatcaaactggctgggttcaccgagattggcttgaagaccttggggtctgacgatgctgacatccgtagagtggctatcgacctcgttcgctctcctactggtatgcagtctggtgcctcaggtaagttcggtgcaacagcttctgacatccatgagagacttcatggtactgaccagcgtacttataatgacttgtacaaagcaatgtctgacgctatgaaagaccctgagttctctactggcggcgctaagatgtcccgtgaagaaactcgatacactatctaccgtagagcggcactagctattgagcgtccagaactacagaaggcactcactccgtctgagagaatcgttatggacatcattaagcgtcactttgacaccaagcgtgaacttatggaaaacccagcaatattcggtaacacaaaggctgtgagtatcttccctgagagtcgccacaaaggtacttacgttcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0134_sequence/1
type: Sequence
persistent identity: BBa Z0134 sequence
display id: BBa_Z0134_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctaacgtaattaaaaccgttttgacttaccagttagatggctccaatcgtgattttaatatcccgtttgagtatctagcccgtaagttcgtagtggtaactcttattggtgtagaccgaaaggtccttacgattaatacagactatcgctttgctacacgtactactatctctctgacaaaggcttggggtccagccgatggctacacgaccatcgagttacgtcgagtaacctccactaccgaccgattggttgactttacggatggttcaatcctccgcgcgtatgaccttaacgtcgctcagattcaaacgatgcacgtagcggaagaggcccgtgacctcactacggatactatcggtgtcaataacgatggtcacttggatgctcgtggtcgtcgaattgtgaacctagcgaacgccgtggatgaccgcgatgctgttccgtttggtcaactaaagaccatgaaccagaactcatggcaagcacgtaatgaagccttacagttccgtaatgaggctgagactttcagaaaccaagcggagggctttaagaacgagtccagtaccaacgctacgaacacaaagcagtggcgcgatgagaccaagggtttccgagacgaagccaagcggttcaagaatacggctggtcaatacgctacatctgctgggaactctgcttccgctgcgcatcaatctgaggtaaacgctgagaactctgccacagcatccgctaactctgctcatttggcagaacagcaagcagaccgtgcggaacgtgaggcagacaagctggaaaattacaatggattggctggtgcaattgataaggtagatggaaccaatgtgtactggaaaggaaatattcacgctaacgggcgcctttacatgaccacaaacggttttgactgtggccagtatcaacagttctttggtggtgtcactaatcgttactctgtcatggagtggggagatgagaacggatggctgatgtatgttcaacgtagagagtggacaacagcgataggcggtaacatccagttagtagtaaacggacagatcatcacccaaggtggagccatgaccggtcagctaaaattgcagaatgggcatgttcttcaattagagtccgcatccgacaaggcgcactatattctatctaaagatggtaacaggaataactggtacattggtagagggtcagataacaacaatgactgtaccttccactcctatgtacatggtacgaccttaacactcaagcaggactatgcagtagttaacaaacacttccacgtaggtcaggccgttgtggccactgatggtaatattcaaggtactaagtggggaggtaaatggctggatgcttacctacgtgacagcttcgttgcgaagtccaaggcgtggactcaggtgtggtctggtagtgctggcggtggggtaagtgtgactgtttcacaggatctccgcttccgcaatatctggattaagtgtgccaacaactcttggaacttcttccgtactggccccgatggaatctacttcatagcctctgatggtggatggttacgattccaaatacactccaacggtctcggattcaagaatattgcagacagtcgttcagtacctaatgcaatcatggtggagaacgagtaacgatcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0136_sequence/1
type: Sequence
persistent identity: BBa Z0136 sequence
display id: BBa_Z0136_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaaaaggataagagccttattacattcttagagatgttggacactgcgatggctcagcgtatgcttgcggacctttcggaccatgagcgtcgctctccgcaactctataatgctattaacaaactgttagaccgccacaagttccagattggtaagttgcagccggatgttcacatcttaggtggccttgctggtgctcttgaagagtacaaagagaaagtcggtgataacggtcttacggatgatgatatttacacattacagtgaagctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0137_sequence/1
type: Sequence
persistent identity: BBa Z0137 sequence
display id: BBa_Z0137_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcacacagtccaatcgaaacgcattagttgtagctcaactgaaaggagacttcgtggcgttcctattcgtcttatggaaggcgctaaacctaccggtgcccactaagtgtcagattgacatggctaaggtgctggcgaatggagacaacaagaagttcatcttacaggctttccgtggtatcggtaagtcgttcatcacatgtgcgttcgttgtgtggtccttatggagagaccctcagttgaagatacttatcgtatcagcctctaaggagcgtgcagacgctaactccatctttattaagaacatcattgacctgctgccattcctatctgagttaaagccaagacccggacagcgtgactcggtaatcagctttgatgtaggcccagccaatcctgaccactctcctagtgtgaaatcagtaggtatcactggtcagttaactggtagccgtgctgacattatcattgcggatgacgttgagattccgtctaacagcgcaactatgggtgcccgtgagaagctatggactctggttcaggagttcgctgcgttacttaaaccgctgccttcctctcgcgttatctaccttggtacacctcagacagagatgactctctataaggaacttgaggataaccgtgggtacacaaccattatctggcctgctctgtacccaaggacacgtgaagagaacctctattactcacagcgtcttgctcctatgttacgcgctgagtacgatgagaaccctgaggcacttgctgggactccaacagacccagtgcgctttgaccgtgatgacctgcgcgagcgtgagttggaatacggtaaggctggctttacgctacagttcatgcttaaccctaaccttagtgatgccgagaagtacccgctgaggcttcgtgacgctatcgtagcggccttagacttagagaaggccccaatgcattaccagtggcttccgaaccgtcagaacatcattgaggaccttcctaacgttggccttaagggtgatgacctgcatacgtaccacgattgttccaacaactcaggtcagtaccaacagaagattctggtcattgaccctagtggtcgcggtaaggacgaaacaggttacgctgtgctgtacacactgaacggttacatctaccttatggaagctggaggtttccgtgatggctactccgataagacccttgagttactcgctaagaaggcaaagcaatggggagtccagacggttgtctacgagagtaacttcggtgacggtatgttcggtaaggtattcagtcctatccttcttaaacaccacaactgtgcgatggaagagattcgtgcccgtggtatgaaagagatgcgtatttgcgatacccttgagccagtcatgcagactcaccgccttgtaattcgtgatgaggtcattagggccgactaccagtccgctcgtgacgtagacggtaagcatgacgttaagtactcgttgttctaccagatgacccgtatcactcgtgagaaaggcgctctggctcatgatgaccgattggatgcccttgcgttaggcattgagtatctccgtgagtccatgcagttggattccgttaaggtcgagggtgaagtacttgctgacttccttgaggaacacatgatgcgtcctacggttgctgctacgcatatcattgagatgtctgtgggaggagttgatgtgtactctgaggacgatgagggttacggtacgtctttcattgagtggtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0155_sequence/1
type: Sequence
persistent identity: BBa Z0155 sequence
display id: BBa_Z0155_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgactataagacctactaaaagtacagactttgaggtattcactccggctcaccatgacattcttgaagctaaggctgctggtattgagccgagtttccctgatgcttccgagtgtgtcacgttgagcctctatgggttccctctagctatcggtggtaactgcggggaccagtgctggttcgttacgagcgaccaagtgtggcgacttagtggaaaggctaagcgaaagttccgtaagttaatcatggagtatcgcgataagatgcttgagaagtatgatactctttggaattacgtatgggtaggcaatacgtcccacattcgtttcctcaagactatcggtgcggtattccatgaagagtacacacgagatggtcaatttcagttatttacaatcacgaaaggcggctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0156_sequence/1
type: Sequence
persistent identity: BBa Z0156 sequence
display id: BBa_Z0156_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgctgggcagcagctataccgattgcaataagtggcgctcaggctatcagtggtcagaacgctcaggccaaaatgattgccgctcagaccgctgctggtcgtcgtcaagctatggaaatcatgaggcagacgaacatccagaatgctgacctatcgttgcaagctcgaagtaaacttgaggaagcgtccgccgagttgacctcacagaacatgcagaaggtccaagctattgggtctatccgagcggctatcggagagagtatgcttgaaggttcctcaatggaccgcattaagcgagtcacagaaggacagttcattcgggaagccaatatggtaactgagaactatcgccgtgactaccaagcaatcttcgcacagcaacttggtggtactcaaagtgctgcaagtcagattgacgaaatctataagagcgaacagaaacagaagagtaagctacagatggttctggacccactggctatcatggggtcttccgctgcgagtgcttacgcatccggtgcgttcgactctaagtccacaactaaggcacctattgttgccgctaaaggaaccaagaccggcaggtaaccgcgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0144_sequence/1
type: Sequence
persistent identity: BBa Z0144 sequence
display id: BBa_Z0144_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctagagttcctccgtaagctaatcccttgggttctcgctgggatgctattcgggttaggatggcatctagggtcagactcaatggacgctaaatggaaacaggaggtacacaatgagtacgttaagagagttgaggctgcgaagagcactcaaagagcaatcgatgcggtatctgctaagtatcaagaagaccttgccgcgctggaagggagcactgataggattatttctgatttgcgtagcgacaataagcggttgcgcgtcagagtcaaaactaccggaacctccgatggtcagtgtggattcgagcctgatggtcgagccgaacttgacgaccgagatgctaaacgtattctcgcagtgacccagaagggtgacgcatggattcgtgcgttacaggatactattcgtgaactgcaacgtaagtagcatgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0157_sequence/1
type: Sequence
persistent identity: BBa Z0157 sequence
display id: BBa_Z0157_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgctatcattagactttaacaacgaattgattaaggctgctccaattgttgggacgggtgtagcagatgttagtgctcgactgttctttgggttaagccttaacgaatggttctacgttgctgctatcgcctacacagtggttcagattggtgccaaggtagtcgataagatgattgactggaagaaagccaataaagaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0148_sequence/1
type: Sequence
persistent identity: BBa Z0148 sequence
display id: BBa_Z0148_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttccgcttattgttgaacctactgcggcatagagtcacctaccgatttcttgtggtactttgtgctgcccttgggtacgcatctcttactggagacctcagttcactggagtctgtcgtttgctctatactcacttgtagcgattag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Z0293_sequence/1
type: Sequence
persistent identity: BBa Z0293 sequence
display id: BBa_Z0293_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtcttcctgaccgactgatggctcaccgagggattcagcggtatgattgcatcacaccacttcatccctatagagtcaagtcctaaggtatacccataaagagcctctaatggtctatcctaaggtctatacctaaagataggccatcctatcagtgtcacctaaagagggtcttagagagggcctatggagttcctatagggtcctttaaaatataccataaaaatctgagtgactatctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z