BBa_Z0119 1 BBa_Z0119 T7.2 gene 3.5 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z Gene 3.5 codes for lysozyme, which is actually not a lysozyme but an N-acetylmuramyl-L-alanine amidase. The most important functions of lysozyme are also not in cell lysis but lie in controlling initiation and termination by T7 RNAP, which in turn affect DNA replication and packaging. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1862116 1 ggt???>GGC range1862116 1 121 123 annotation1849938 1 S3: possible promoter range1849938 1 110 173 annotation1860956 1 S3 -35 region range1860956 1 123 127 annotation1862117 1 ttt->TTC range1862117 1 145 147 annotation1860957 1 S3 -10 region range1860957 1 147 152 BBa_Z0156 1 BBa_Z0156 T7.2 gene 14 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z Mutations on 3' end to remove Gene 15 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855267 1 g->C mutation range1855267 1 585 585 annotation1881264 1 tgt...ggc->tgc...ggc codon shuffle range1881264 1 4 39 annotation1884022 1 added SacII range1884022 1 592 597 annotation1855266 1 g->C mutation range1855266 1 582 582 BBa_Z0144 1 BBa_Z0144 T7.2 gene 18.5 (18.7) 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z lysis associated protein. Homologs of 18.5/18.7 are found in all phages (in T1 the two proteins are fused); contains gene 18.7 within coding domain. false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1883680 1 added CATGC to form SphI range1883680 1 434 437 annotation1862602 1 E promoter remnant range1862602 1 1 34 annotation1862600 1 gaattttta->gagttcctc E-10 range1862600 1 7 15 annotation1855761 1 gene 18.7 range1855761 1 116 367 annotation1862601 1 E -10 region range1862601 1 8 13 BBa_Z0134 1 BBa_Z0134 T7.2 gene 17 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z T7 tail fiber protein of phage capsid (adsorption); 14 +/- 3 (eighteen expected by symmettry) copies per virion; 3 copies per tail fiber false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1884038 1 added PvuI range1884038 1 1663 1668 BBa_Z0152 1 BBa_Z0152 T7.2 gene 2.5 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z mutation from T7 to remove RBS from gene 2.8 false false _10_ 0 64 11 Not in stock false false Sriram Kosuri annotation1849934 1 S2: possible promoter range1849934 1 3 66 annotation1860894 1 S2 -10 region range1860894 1 40 45 annotation1881251 1 ggc...gct->ggc..gct codon shuffle range1881251 1 4 39 annotation1860895 1 ttc -> TTT range1860895 1 16 18 annotation1860893 1 S2 -35 region range1860893 1 16 20 annotation1849919 1 a->G mutation to remove RBS range1849919 1 690 690 BBa_Z0106 1 BBa_Z0106 T7.2 gene 0.7 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation Gene 0.7 codes for a protein that inactivates host-catalyzed transcription, it is also a serine-threonine protein kinase. The two activities are separable. Gp0.7 phosphorylates many host proteins, including ribosome components, ribonucleases III and E, and the β and β?? subunits of E. coli RNAP. The BR3 and Y49 strains contain the rpoC-E1158K mutation, which renders gp0.7 essential for phage growth. The mutation affects the region of β?? to which the T7 protein gp2 binds. Gene 0.7 is non-essential in wild-type hosts and is actually detrimental when T7 is grown at 30˚C in rich media. Early T7 RNA and protein synthesis is prolonged after infection by gp0.7 mutants. However, phage growth at elevated temperatures or in minimal media, or in cells harboring the Col Ib plasmid renders gene 0.7 essential. The processes underlying the conditional necessity for gp0.7 function have not been determined. The E. coli C promoter -35 region is located on the 3' end of the coding sequence. Removing the downstream region will probably be sufficient to delete functioning of the promoter. false false _10_ 0 250 10 Not in stock false false T7.2 annotation1883644 1 added BsiWI range1883644 1 1081 1086 annotation1476455 1 -35 region of C promoter range1476455 1 1058 1062 annotation1476456 1 -45 region of C promoter range1476456 1 1049 1049 BBa_Z0293 1 BBa_Z0293 T7 Right End 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Right End of T7 DNA. Important in packaging. Contains short terminal repeat region and the right terminal repeat. Contains sequence starting after gene 19.5 stop site. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875190 1 Right Terminal Repeat range1875190 1 240 399 annotation1875189 1 Short Terminal Repeat Region range1875189 1 68 226 BBa_Z0108 1 BBa_Z0108 T7.2 gene 1.1 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation open reading frame for gene 1.1. from wild-type T7 genome false false _10_ 0 250 10 Not in stock false false T7.2 BBa_Z0159 1 BBa_Z0159 T7.2 gene 6.5 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z silent mutation at 3' end to remove gene 6.7 RBS. -35 region of F1 probably non-functional at 5' end of gene. false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855744 1 g->C mutation range1855744 1 246 246 annotation1855743 1 g->A mutation range1855743 1 249 249 BBa_Z0139 1 BBa_Z0139 T7.2 gene 4.5 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z conserved; non-essential false false _11_ 0 64 11 Not in stock false false Sriram Kosuri BBa_Z0294 1 BBa_Z0294 A1,A2,A3,boxA 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Host promoter region containing 3 promoters and boxA antitermination site. Takes natural sequence from end of phi0L to start of R0.3 false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875194 1 boxA antitermination site range1875194 1 400 409 annotation1875192 1 A2 range1875192 1 164 227 annotation1875193 1 A3 range1875193 1 289 351 annotation1883627 1 add gctc to give SacI range1883627 1 432 435 annotation1875191 1 A1 range1875191 1 37 99 BBa_Z0124 1 BBa_Z0124 T7.2 gene 7.3 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Gene 7 was originally defined by mutations that mapped between genes 6 and 8 but was subsequently shown to be two genes, 7 and 7.3. Gp7.3 is a tail protein, present in ~30 copies per particle, that is essential for infectivity though not particle formation. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855232 1 possible promoter site? range1855232 1 168 168 annotation1883626 1 att->ATC range1883626 1 295 297 annotation1883678 1 added bstEII range1883678 1 301 307 BBa_Z0125 1 BBa_Z0125 T7.2 gene 8 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Head-tail connector. 12 copies per virion. Three possible host promoters with -35 regions at 293, 1100, 1249 false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1883674 1 added SalI range1883674 1 1612 1617 annotation1855239 1 possible promoter site? range1855239 1 1425 1425 BBa_Z0136 1 BBa_Z0136 T7.2 gene 18 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z small terminase subunit false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1884021 1 added AGCTT to form HindIII range1884021 1 271 275 BBa_Z0505 1 BBa_Z0505 T7.2 Revision E 2006-07-10T11:00:00Z 2015-05-08T01:14:55Z T7.2 Revision D, modified by adding restriction sites T7.2 Revision E, see http://openwetware.org/wiki/T7.2 false false _11_ 0 64 10 Not in stock false tried to apportion more single cutters across genome false Sriram Kosuri component2243423 1 BBa_Z0253 component2243631 1 BBa_Z0157 component2243650 1 BBa_Z0251 component2243531 1 BBa_Z0253 component2243535 1 BBa_Z0139 component2243509 1 BBa_Z0273 component2243528 1 BBa_Z0120 component2243655 1 BBa_Z0293 component2243562 1 BBa_Z0262 component2243513 1 BBa_Z0252 component2243606 1 BBa_Z0262 component2243652 1 BBa_Z0148 component2243500 1 BBa_Z0153 component2243610 1 BBa_Z0262 component2243624 1 BBa_Z0251 component2243507 1 BBa_Z0119 component2243644 1 BBa_Z0262 component2243643 1 BBa_Z0144 component2243431 1 BBa_Z0273 component2243585 1 BBa_Z0251 component2243498 1 BBa_Z0262 component2243438 1 BBa_Z0158 component2243553 1 BBa_Z0261 component2243486 1 BBa_Z0116 component2243420 1 BBa_Z0292 component2243479 1 BBa_Z0262 component2243617 1 BBa_Z0132 component2243590 1 BBa_Z0127 component2243534 1 BBa_Z0261 component2243586 1 BBa_Z0261 component2243432 1 BBa_Z0261 component2243483 1 BBa_Z0261 component2243440 1 BBa_Z0273 component2243596 1 BBa_Z0262 component2243456 1 BBa_Z0107 component2243473 1 BBa_Z0109 component2243514 1 BBa_Z0262 component2243651 1 BBa_Z0261 component2243490 1 BBa_Z0262 component2243616 1 BBa_Z0262 component2243448 1 BBa_Z0262 component2243601 1 BBa_Z0129 component2243636 1 BBa_Z0273 component2243462 1 BBa_Z0108 component2243628 1 BBa_Z0262 component2243445 1 BBa_Z0106 component2243489 1 BBa_Z0253 component2243460 1 BBa_Z0291 component2243434 1 BBa_Z0301 component2243632 1 BBa_Z0261 component2243542 1 BBa_Z0261 component2243566 1 BBa_Z0261 component2243593 1 BBa_Z0261 component2243533 1 BBa_Z0138 component2243463 1 BBa_Z0261 component2243561 1 BBa_Z0251 component2243537 1 BBa_Z0273 component2243648 1 BBa_Z0137 component2243625 1 BBa_Z0262 component2243637 1 BBa_Z0261 component2243546 1 BBa_Z0261 component2243559 1 BBa_Z0273 component2243471 1 BBa_Z0261 component2243627 1 BBa_Z0134 component2243541 1 BBa_Z0252 component2243615 1 BBa_Z0156 component2243429 1 BBa_Z0294 component2243464 1 BBa_Z0115 component2243552 1 BBa_Z0160 component2243573 1 BBa_Z0262 component2243634 1 BBa_Z0136 component2243470 1 BBa_Z0273 component2243605 1 BBa_Z0251 component2243567 1 BBa_Z0123 component2243595 1 BBa_Z0128 component2243557 1 BBa_Z0154 component2243583 1 BBa_Z0126 component2243478 1 BBa_Z0253 component2243618 1 BBa_Z0262 component2243578 1 BBa_Z0251 component2243609 1 BBa_Z0155 component2243565 1 BBa_Z0159 component2243447 1 BBa_Z0273 component2243622 1 BBa_Z0133 component2243592 1 BBa_Z0272 component2243545 1 BBa_Z0121 component2243568 1 BBa_Z0262 component2243461 1 BBa_Z0262 component2243579 1 BBa_Z0262 component2243603 1 BBa_Z0273 component2243475 1 BBa_Z0271 component2243532 1 BBa_Z0261 component2243482 1 BBa_Z0151 component2243572 1 BBa_Z0124 component2243576 1 BBa_Z0125 component2243441 1 BBa_Z0262 component2243468 1 BBa_Z0252 component2243497 1 BBa_Z0152 component2243435 1 BBa_Z0261 component2243501 1 BBa_Z0261 annotation2243423 1 BBa_Z0253 range2243423 1 378 412 annotation2243615 1 BBa_Z0156 range2243615 1 24950 25546 annotation2243473 1 BBa_Z0109 range2243473 1 6666 7751 annotation2243486 1 BBa_Z0116 range2243486 1 8455 8655 annotation2243651 1 BBa_Z0261 range2243651 1 36371 36390 annotation2243605 1 BBa_Z0251 range2243605 1 24458 24492 annotation2243606 1 BBa_Z0262 range2243606 1 24493 24512 annotation2243559 1 BBa_Z0273 range2243559 1 16451 16518 annotation2243440 1 BBa_Z0273 range2243440 1 2036 2103 annotation2243593 1 BBa_Z0261 range2243593 1 21370 21389 annotation2243461 1 BBa_Z0262 range2243461 1 6116 6135 annotation2243622 1 BBa_Z0133 range2243622 1 27831 31787 annotation2243576 1 BBa_Z0125 range2243576 1 17463 19079 annotation2243541 1 BBa_Z0252 range2243541 1 12817 12851 annotation2243655 1 BBa_Z0293 range2243655 1 36541 36939 annotation2243578 1 BBa_Z0251 range2243578 1 19080 19114 annotation2243497 1 BBa_Z0152 range2243497 1 8711 9409 annotation2243561 1 BBa_Z0251 range2243561 1 16519 16553 annotation2243648 1 BBa_Z0137 range2243648 1 34575 36335 annotation2243628 1 BBa_Z0262 range2243628 1 33511 33530 annotation2243438 1 BBa_Z0158 range2243438 1 1676 2035 annotation2243535 1 BBa_Z0139 range2243535 1 12479 12748 annotation2243652 1 BBa_Z0148 range2243652 1 36391 36540 annotation2243609 1 BBa_Z0155 range2243609 1 24513 24929 annotation2243462 1 BBa_Z0108 range2243462 1 6136 6264 annotation2243566 1 BBa_Z0261 range2243566 1 16829 16848 annotation2243489 1 BBa_Z0253 range2243489 1 8656 8690 annotation2243595 1 BBa_Z0128 range2243595 1 21390 21980 annotation2243625 1 BBa_Z0262 range2243625 1 31823 31842 annotation2243542 1 BBa_Z0261 range2243542 1 12852 12871 annotation2243507 1 BBa_Z0119 range2243507 1 9900 10360 annotation2243650 1 BBa_Z0251 range2243650 1 36336 36370 annotation2243445 1 BBa_Z0106 range2243445 1 2124 3209 annotation2243431 1 BBa_Z0273 range2243431 1 848 915 annotation2243528 1 BBa_Z0120 range2243528 1 10484 12190 annotation2243632 1 BBa_Z0261 range2243632 1 33735 33754 annotation2243498 1 BBa_Z0262 range2243498 1 9410 9429 annotation2243553 1 BBa_Z0261 range2243553 1 15522 15541 annotation2243637 1 BBa_Z0261 range2243637 1 34098 34117 annotation2243509 1 BBa_Z0273 range2243509 1 10361 10428 annotation2243636 1 BBa_Z0273 range2243636 1 34030 34097 annotation2243500 1 BBa_Z0153 range2243500 1 9430 9879 annotation2243463 1 BBa_Z0261 range2243463 1 6265 6284 annotation2243631 1 BBa_Z0157 range2243631 1 33531 33734 annotation2243501 1 BBa_Z0261 range2243501 1 9880 9899 annotation2243464 1 BBa_Z0115 range2243464 1 6285 6542 annotation2243590 1 BBa_Z0127 range2243590 1 20114 21315 annotation2243567 1 BBa_Z0123 range2243567 1 16849 17115 annotation2243562 1 BBa_Z0262 range2243562 1 16554 16573 annotation2243546 1 BBa_Z0261 range2243546 1 14993 15012 annotation2243468 1 BBa_Z0252 range2243468 1 6543 6577 annotation2243429 1 BBa_Z0294 range2243429 1 413 847 annotation2243460 1 BBa_Z0291 range2243460 1 5956 6115 annotation2243586 1 BBa_Z0261 range2243586 1 20094 20113 annotation2243603 1 BBa_Z0273 range2243603 1 24390 24457 annotation2243420 1 BBa_Z0292 range2243420 1 1 377 annotation2243573 1 BBa_Z0262 range2243573 1 17443 17462 annotation2243583 1 BBa_Z0126 range2243583 1 19135 20058 annotation2243592 1 BBa_Z0272 range2243592 1 21316 21369 annotation2243572 1 BBa_Z0124 range2243572 1 17136 17442 annotation2243478 1 BBa_Z0253 range2243478 1 7789 7823 annotation2243568 1 BBa_Z0262 range2243568 1 17116 17135 annotation2243470 1 BBa_Z0273 range2243470 1 6578 6645 annotation2243552 1 BBa_Z0160 range2243552 1 15013 15521 annotation2243448 1 BBa_Z0262 range2243448 1 3278 3297 annotation2243456 1 BBa_Z0107 range2243456 1 3298 5955 annotation2243624 1 BBa_Z0251 range2243624 1 31788 31822 annotation2243531 1 BBa_Z0253 range2243531 1 12191 12225 annotation2243537 1 BBa_Z0273 range2243537 1 12749 12816 annotation2243490 1 BBa_Z0262 range2243490 1 8691 8710 annotation2243483 1 BBa_Z0261 range2243483 1 8435 8454 annotation2243471 1 BBa_Z0261 range2243471 1 6646 6665 annotation2243533 1 BBa_Z0138 range2243533 1 12246 12458 annotation2243617 1 BBa_Z0132 range2243617 1 25567 27810 annotation2243557 1 BBa_Z0154 range2243557 1 15542 16450 annotation2243579 1 BBa_Z0262 range2243579 1 19115 19134 annotation2243585 1 BBa_Z0251 range2243585 1 20059 20093 annotation2243447 1 BBa_Z0273 range2243447 1 3210 3277 annotation2243534 1 BBa_Z0261 range2243534 1 12459 12478 annotation2243565 1 BBa_Z0159 range2243565 1 16574 16828 annotation2243434 1 BBa_Z0301 range2243434 1 936 1655 annotation2243482 1 BBa_Z0151 range2243482 1 7844 8434 annotation2243618 1 BBa_Z0262 range2243618 1 27811 27830 annotation2243627 1 BBa_Z0134 range2243627 1 31843 33510 annotation2243644 1 BBa_Z0262 range2243644 1 34555 34574 annotation2243643 1 BBa_Z0144 range2243643 1 34118 34554 annotation2243545 1 BBa_Z0121 range2243545 1 12872 14992 annotation2243532 1 BBa_Z0261 range2243532 1 12226 12245 annotation2243514 1 BBa_Z0262 range2243514 1 10464 10483 annotation2243475 1 BBa_Z0271 range2243475 1 7752 7788 annotation2243435 1 BBa_Z0261 range2243435 1 1656 1675 annotation2243479 1 BBa_Z0262 range2243479 1 7824 7843 annotation2243513 1 BBa_Z0252 range2243513 1 10429 10463 annotation2243610 1 BBa_Z0262 range2243610 1 24930 24949 annotation2243432 1 BBa_Z0261 range2243432 1 916 935 annotation2243596 1 BBa_Z0262 range2243596 1 21981 22000 annotation2243616 1 BBa_Z0262 range2243616 1 25547 25566 annotation2243441 1 BBa_Z0262 range2243441 1 2104 2123 annotation2243634 1 BBa_Z0136 range2243634 1 33755 34029 annotation2243601 1 BBa_Z0129 range2243601 1 22001 24389 BBa_Z0148 1 BBa_Z0148 T7.2 gene 19.5 2006-05-03T11:00:00Z 2015-05-08T01:14:54Z non-essential; conserved false false _11_ 0 64 11 Not in stock false false Sriram Kosuri BBa_Z0133 1 BBa_Z0133 T7.2 gene 16 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Internal core protein of phage capsid; ~4 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855274 1 S6: possible promoter range1855274 1 3876 3939 annotation1862595 1 aagatttat->AAAATCTAC S6-10 range1862595 1 3910 3918 annotation1862596 1 ttgact->CTGACC S6-35 range1862596 1 3889 3894 BBa_Z0115 1 BBa_Z0115 T7.2 gene 1.2 2006-04-11T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation Gene 1.2 inhibits E. coli dGTPase and converts it into a rGTP-binding protein, which is important for T7 only when dgt is over-expressed (optA1 mutation). T7 gene 1.2 also causes phage exclusion by the F plasmid, but T3 gene 1.2 overcomes the exclusion system. false false _10_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0292 1 BBa_Z0292 T7 Left End 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Left end of T7 genome. Includes terminal repeat, short repeats, leftward host promoter A0. Contains sequence up to -27bp of T7 promoter phi0L. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875186 1 left terminal repeat range1875186 1 1 160 annotation1875188 1 A0 range1875188 1 210 272 annotation1875187 1 short left terminal repeat region range1875187 1 175 338 BBa_Z0126 1 BBa_Z0126 T7.2 gene 9 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Capsid scaffolding protein false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855247 1 possible promoter site? range1855247 1 671 671 annotation1881254 1 ggc...ggc -> ggc...ggc codon shuffle range1881254 1 4 39 annotation1855246 1 possible promoter site? range1855246 1 522 522 BBa_Z0291 1 BBa_Z0291 T7.2 primary origin of replication 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z -- No description -- false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862808 1 T7 phi1.1A promoter range1862808 1 2 36 annotation1862810 1 R1.1 stem loop range1862810 1 28 80 annotation1862809 1 T7 phi1.1B promoter range1862809 1 77 111 BBa_Z0151 1 BBa_Z0151 T7.2 gene 1.7 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z Bases have been changed to remove gene 1.8 start site acataaAgataaatgttaCgcataa false false _10_ 0 64 11 Not in stock false false Sriram Kosuri annotation1849888 1 g->A to remove gene 1.8 RBS range1849888 1 573 573 annotation1849887 1 t->C to remove gene 1.8 start range1849887 1 585 585 BBa_Z0120 1 BBa_Z0120 T7.2 gene 4A/4B 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z The full-length gene 4 protein is a 5??->3?? helicase of the E. coli DnaB family and a DNA primase. An in-frame internal start site within gene 4 results in a protein, gp4B, that lacks the N-terminal 63 amino acids of the 63 kDa gp4A, and consequently lacks primase activity. The primase recognition sites on ssDNA are 5??-G/TGGTC, and 5??-GTGTC. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881252 1 gac...tac -> gat ... tat codon shuffle range1881252 1 4 39 annotation1849950 1 phi4c range1849950 1 1080 1114 annotation1849949 1 S4: possible promoter range1849949 1 574 637 annotation1883672 1 added ngoMIV range1883672 1 1702 1707 annotation1862192 1 ttt->TTC S4 -35 range1862192 1 586 588 annotation1862194 1 codon shuffled (13 changes) phi4c range1862194 1 1078 1116 annotation1849946 1 gene 4B range1849946 1 190 1701 annotation1862191 1 cat->CAC 4.2 start range1862191 1 1423 1425 annotation1849948 1 gene 4.2 range1849948 1 1424 1762 annotation1862190 1 gat->gac 4.1 start range1862190 1 70 72 annotation1849947 1 gene 4.1 range1849947 1 71 193 annotation1849942 1 gene 4A range1849942 1 1 1701 annotation1862193 1 tta->CUG S4-10 range1862193 1 610 612 BBa_Z0251 1 BBa_Z0251 T7 strong promoter 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z bacteriophage T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476333 1 consensus sequence range1476333 1 6 28 BBa_Z0127 1 BBa_Z0127 T7.2 gene 10A/B 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z major/minor capsid protein. The 10B portion is non-essential, 10A-only phages seem to grow normally. ~430 copies of 10A and 16 copies of 10B in capsid (415 expected from symmetry). JJ Dunn removed various pieces of terminator.. if you remove hairpin, the gene 10 RNA is obliterated. Also, got rid of gene 10 pre-shine dalgarno and could not get translation off gene 10. (Dunn JJ, PC) false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1850774 1 gene 10B range1850774 1 1 1196 annotation1850773 1 gene 10A range1850773 1 1 1038 annotation1883679 1 added XmaI range1883679 1 1197 1202 BBa_Z0128 1 BBa_Z0128 T7.2 gene 11 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z structural tail protein of phage capsid; 12 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881255 1 cgc...gag ->cga...gaa codon shuffle range1881255 1 4 39 BBa_Z0132 1 BBa_Z0132 T7.2 gene 15 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z internal core protein of phage capsid; ~8 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0153 1 BBa_Z0153 T7.2 gene 3 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z Mutations to remove gene 3.5 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1883676 1 aggaaaggagga->CgCaaGggGggG removes RBS range1883676 1 430 441 BBa_Z0273 1 BBa_Z0273 T7 R0.3 RNAseIII site 2006-05-29T11:00:00Z 2015-05-08T01:14:55Z Contains 9 bp before and 10 bp after stem loop(up to start of 0.3 RBS). false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1875207 1 R0.3 stem loop range1875207 1 10 58 BBa_Z0272 1 BBa_Z0272 T7.2 Tphi transcriptional terminator 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Taken 10 bp before expected start of stem loop structure, and 3bp after expected stop of transcription (7bp after stem loop) false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862785 1 terminator stem loop range1862785 1 11 46 BBa_Z0154 1 BBa_Z0154 T7.2 gene 6 2006-04-30T11:00:00Z 2015-05-08T01:14:54Z 3' end mutated to remove gene 6.3 startsite and alter ribosome binding site false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1850750 1 g->C mutation to remove gene 6.3 start range1850750 1 891 891 annotation1850751 1 g->A mutation to alter gene 6.3 RBS range1850751 1 882 882 annotation1884018 1 added xhoI range1884018 1 904 909 BBa_Z0155 1 BBa_Z0155 T7.2 gene 13 2006-05-03T11:00:00Z 2015-05-08T01:14:54Z Mutations from wild-type on 3' end to mutate Gene 14 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855258 1 g->C mutation range1855258 1 411 411 annotation1855259 1 g->C mutation range1855259 1 414 414 BBa_Z0129 1 BBa_Z0129 T7.2 gene 12 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z a structural tail protein of the phage capsid; 6 copies per virion false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881263 1 gca...ggt->gca...ggc codon shuffle range1881263 1 4 39 annotation1881262 1 gtt->GTG F2-35 range1881262 1 382 384 annotation1883677 1 added ttaa to form PacI range1883677 1 2386 2389 annotation1881261 1 F2 range1881261 1 337 399 BBa_Z0271 1 BBa_Z0271 T7.2 TE transcriptional terminator 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Takes 6bp ahead of the expected start of the stem loop, and 3bp after the expected termination site (11bp after end of stem loop). Causes termination by E. coli RNA polymerase (but not T7 RNA polymerase). false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862801 1 TE expected stem loop range1862801 1 7 26 BBa_Z0157 1 BBa_Z0157 T7.2 gene 17.5 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z Mutations on 3' end to remove gene 18 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1855282 1 g->A mutation range1855282 1 198 198 annotation1855281 1 g->A mutation range1855281 1 201 201 BBa_Z0138 1 BBa_Z0138 T7.2 gene 4.3 2006-04-26T11:00:00Z 2015-05-08T01:14:54Z conserved; non-essential false false _11_ 0 64 11 Not in stock false false Sriram Kosuri BBa_Z0123 1 BBa_Z0123 T7.2 gene 6.7 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z Head protein. ~18 copies per virion. Gp6.7 is not absolutely essential, morphological normal T7 particles are produced in its absence but have reduced infectivity. false false _11_ 0 64 11 Not in stock false false Sri Kosuri BBa_Z0107 1 BBa_Z0107 T7.2 gene 1 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation Encodes gp1: T7 RNA polymerase from wild-type T7; Suppressor mutation in 19 allows no gene 1 late in infection. It's thought that gene 1 is hanging out at the CJ terminator at the pause site... recruiting 18 and 19 after packaging. (Studier FW, PC) false false _10_ 0 250 10 Not in stock false false T7.2 annotation1860880 1 gat -> GAC range1860880 1 2518 2520 annotation1883668 1 added BspEI range1883668 1 2653 2658 annotation1476460 1 S1 promoter startsite range1476460 1 2532 2532 annotation1476462 1 S1 -35 site range1476462 1 2498 2502 annotation1881041 1 aac...gac->aat..gat range1881041 1 4 39 annotation1860879 1 gttgac -> GTAGAT range1860879 1 2497 2502 annotation1476461 1 S1 -10 site range1476461 1 2520 2525 BBa_Z0121 1 BBa_Z0121 T7.2 gene 5 2006-04-24T11:00:00Z 2015-05-08T01:14:54Z T7 DNA Polymerase consists of two proteins, the gene 5 protein and the processivity factor E. coli thioredoxin. Thioredoxin forms a 1:1 complex with the T7 protein and increases its processivity by at least 1000-fold, the complex also increases the activity of the associated 5??->3?? exonuclease on dsDNA but has little effect on ssDNA exonucleolytic activity. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1881253 1 atc...gag -> atc...gaa codon shuffle range1881253 1 4 39 annotation1883673 1 added pstI range1883673 1 2116 2121 BBa_Z0252 1 BBa_Z0252 T7 weak binding and processivity 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476343 1 23bp conserved region range1476343 1 6 28 annotation1476344 1 tga -> acg mutation from consensus range1476344 1 10 12 annotation1476345 1 t -> a mutation from consensus range1476345 1 21 21 BBa_Z0301 1 BBa_Z0301 Venus YFP - E. coli codon optimized 2006-06-20T11:00:00Z 2015-05-08T01:14:55Z Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab. Brighter Variant than standard EYFP. false false _11_ 0 64 10 It's complicated false None really false Sriram Kosuri annotation1881736 1 gtc ...gtt -> gta ... gtt codon shuffle range1881736 1 4 39 BBa_Z0158 1 BBa_Z0158 T7.2 gene 0.3 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z 3' end of gene mutated to remove gene 0.4 RBS false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1883643 1 add apaI range1883643 1 355 360 annotation1855703 1 t->C mutation range1855703 1 345 345 BBa_Z0253 1 BBa_Z0253 T7 weak binding promoter 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z -- No description -- false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri annotation1862691 1 ag->GA from concensus range1862691 1 31 32 annotation1862684 1 ag->TA from concensus range1862684 1 27 28 BBa_Z0137 1 BBa_Z0137 T7.2 gene 19 (19.2, 19.3) 2006-04-25T11:00:00Z 2015-05-08T01:14:54Z large terminase subunit. binds capsid pro-head. Also contains within the sequence for 19.2 and 19.3 within the gene. false false _11_ 0 64 11 Not in stock false false Sri Kosuri annotation1855298 1 gene 19.2 range1855298 1 647 904 annotation1855299 1 gene 19.3 range1855299 1 1184 1357 annotation1881265 1 tct...gcg -> agc...gct codon shuffle range1881265 1 4 39 BBa_Z0262 1 BBa_Z0262 Medium strength T7.2 RBS 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri BBa_Z0261 1 BBa_Z0261 Strong T7.2 RBS 2006-05-15T11:00:00Z 2015-05-08T01:14:55Z Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs. false false _11_10_ 0 64 10 Not in stock false false Sriram Kosuri BBa_Z0160 1 BBa_Z0160 T7.2 gene 5.5-5.7 2006-05-03T11:00:00Z 2015-05-08T01:14:55Z Removes the overlap of gene 5.9 RBS. Not the actual sequence in the design of T7.1, allows the frameshift again. false false _11_ 0 64 11 Not in stock false false Sriram Kosuri annotation1862272 1 gene 5.7 range1862272 1 300 509 annotation1862271 1 gene 5.5 range1862271 1 1 300 annotation1855777 1 a->C mutation range1855777 1 497 497 annotation1862270 1 gene 5.5-5.7 fusion range1862270 1 1 509 annotation1855776 1 a->G mutation range1855776 1 500 500 BBa_Z0116 1 BBa_Z0116 T7.2 gene 2 2006-04-11T11:00:00Z 2015-05-08T01:14:54Z Gp2 binds to and is an inhibitor of E. coli RNAP but in K-12 or B strains the defects due to a lack of gene 2 activity are reduced DNA replication and premature breakdown of replicating DNA, specifically at the left genome end where the major E. coli promoters are found. false false _10_ 0 64 11 Not in stock false false Sri Kosuri annotation1881250 1 tca...aat->agc...aat range1881250 1 4 39 annotation1883670 1 added ecoRI range1883670 1 196 201 BBa_Z0109 1 BBa_Z0109 T7 gene 1.3 2005-04-04T11:00:00Z 2015-05-08T01:14:54Z T7 genome reannotation The ATP-dependent DNA ligase is only essential in E. coli lig mutants, but its presence enhances phage growth in wild-type hosts. false false _10_ 0 250 10 Not in stock false false T7.2 annotation1883669 1 added eagI range1883669 1 1081 1086 BBa_Z0294_sequence 1 caacttaaagagacttaaaagattaatttaaaatttatcaaaaagagtattgacttaaagtctaacctataggatacttacagccatcgagagggacacggcgaatagccatcccaatcgacaccggggtcaaccggataagtagacagcctgataagtcgcacgaaaaacaggtattgacaacatgaagtaacatgcagtaagatacaaatcgctaggtaacactagcagcgtcaaccgggcgcacagtgccttctaggtgacttaagcgcaccacggcacataaggtgaaacaaaacggttgacaacatgaagtaaacacggtacgatgtaccacatgaaacgacagtgagtcaccacactgaaaggtgatgcggtctaacgaaacctgacctaagacgctctttaacaatctggtaaatagctcttgagctc BBa_Z0148_sequence 1 atgttccgcttattgttgaacctactgcggcatagagtcacctaccgatttcttgtggtactttgtgctgcccttgggtacgcatctcttactggagacctcagttcactggagtctgtcgtttgctctatactcacttgtagcgattag BBa_Z0155_sequence 1 atgatgactataagacctactaaaagtacagactttgaggtattcactccggctcaccatgacattcttgaagctaaggctgctggtattgagccgagtttccctgatgcttccgagtgtgtcacgttgagcctctatgggttccctctagctatcggtggtaactgcggggaccagtgctggttcgttacgagcgaccaagtgtggcgacttagtggaaaggctaagcgaaagttccgtaagttaatcatggagtatcgcgataagatgcttgagaagtatgatactctttggaattacgtatgggtaggcaatacgtcccacattcgtttcctcaagactatcggtgcggtattccatgaagagtacacacgagatggtcaatttcagttatttacaatcacgaaaggcggctaa BBa_Z0251_sequence 1 gaaattaatacgactcactatagggagaccacaac BBa_Z0159_sequence 1 atgttaacacctattaaccaattacttaagaaccctaacgatattccagatgtacctcgtgcaaccgctgagtatctacaggttcgattcaactatgcgtacctcgaagcgtctggtcatataggacttatgcgtgctaatggttgtagtgaggcccacatcttgggtttcattcagggcctacagtatgcctctaacgtcattgacgagattgagttacgcaaggaacaactaagagatgatggcgaagattga BBa_Z0106_sequence 1 atgaacattaccgacatcatgaacgctatcgacgcaatcaaagcactgccaatctgtgaacttgacaagcgtcaaggtatgcttatcgacttactggtcgagatggtcaacagcgagacgtgtgatggcgagctaaccgaactaaatcaggcacttgagcatcaagattggtggactaccttgaagtgtctcacggctgacgcagggttcaagatgctcggtaatggtcacttctcggctgcttatagtcacccgctgctacctaacagagtgattaaggtgggctttaagaaagaggattcaggcgcagcctataccgcattctgccgcatgtatcagggtcgtcctggtatccctaacgtctacgatgtacagcgccacgctggatgctatacggtggtacttgacgcacttaaggattgcgagcgtttcaacaatgatgcccattataaatacgctgagattgcaagcgacatcattgattgcaattcggatgagcatgatgagttaactggatgggatggtgagtttgttgaaacttgtaaactaatccgcaagttctttgagggcatcgcctcattcgacatgcatagcgggaacatcatgttctcaaatggagacgtaccatacatcaccgacccggtatcattctcgcagaagaaagacggtggcgcattcagcatcgaccctgaggaactcatcaaggaagtcgaggaagtcgcacgacagaaagaaattgaccgcgctaaggcccgtaaagaacgtcacgaggggcgcttagaggcacgcagattcaaacgtcgcaaccgcaaggcacgtaaagcacacaaagctaagcgcgaaagaatgcttgctgcgtggcgatgggctgaacgtcaagaacggcgtaaccatgaggtagctgtagatgtactaggaagaaccaataacgctatgctctgggtcaacatgttctctggggactttaaggcgcttgaggaacgaatcgcgctgcactggcgtaatgctgaccggatggctatcgctaatggtcttacgctcaacattgataagcaacttgacgcaatgttaatgggctgacgtacg BBa_Z0133_sequence 1 atggataagtacgataagaacgtaccaagtgattatgatggtctgttccaaaaggctgctgatgccaacggggtctcttatgaccttttacgtaaagtcgcttggacagaatcacgatttgtgcctacagcaaaatctaagactggaccattaggcatgatgcaatttaccaaggcaaccgctaaggccctcggtctgcgagttaccgatggtccagacgacgaccgactgaaccctgagttagctattaatgctgccgctaagcaacttgcaggtctggtagggaagtttgatggcgatgaactcaaagctgcccttgcgtacaaccaaggcgagggacgcttgggtaatccacaacttgaggcgtactctaagggagacttcgcatcaatctctgaggagggacgtaactacatgcgtaaccttctggatgttgctaagtcacctatggctggacagttggaaacttttggtggcataaccccaaagggtaaaggcattccggctgaggtaggattggctggaattggtcacaagcagaaagtaacacaggaacttcctgagtccacaagttttgacgttaagggtatcgaacaggaggctacggcgaaaccattcgccaaggacttttgggagacccacggagaaacacttgacgagtacaacagtcgttcaaccttcttcggattcaaaaatgctgccgaagctgaactctccaactcagtcgctgggatggctttccgtgctggtcgtctcgataatggttttgatgtgtttaaagacaccattacgccgactcgctggaactctcacatctggactccagaggagttagagaagattcgaacagaggttaagaaccctgcgtacatcaacgttgtaactggtggttcccctgagaacctcgatgacctcattaaattggctaacgagaactttgagaatgactcccgcgctgccgaggctggcctaggtgccaaactgagtgctggtattattggtgctggtgtggacccgcttagctatgttcctatggtcggtgtcactggtaagggctttaagttaatcaataaggctcttgtagttggtgccgaaagtgctgctctgaacgttgcatccgaaggtctccgtacctccgtagctggtggtgacgcagactatgcgggtgctgccttaggtggctttgtgtttggcgcaggcatgtctgcaatcagtgacgctgtagctgctggactgaaacgcagtaaaccagaagctgagttcgacaatgagttcatcggtcctatgatgcgattggaagcccgtgagacagcacgaaacgccaactctgcggacctctctcggatgaacactgagaacatgaagtttgaaggtgaacataatggtgtcccttatgaggacttaccaacagagagaggtgccgtggtgttacatgatggctccgttctaagtgcaagcaacccaatcaaccctaagactctaaaagagttctccgaggttgaccctgagaaggctgcgcgaggaatcaaactggctgggttcaccgagattggcttgaagaccttggggtctgacgatgctgacatccgtagagtggctatcgacctcgttcgctctcctactggtatgcagtctggtgcctcaggtaagttcggtgcaacagcttctgacatccatgagagacttcatggtactgaccagcgtacttataatgacttgtacaaagcaatgtctgacgctatgaaagaccctgagttctctactggcggcgctaagatgtcccgtgaagaaactcgatacactatctaccgtagagcggcactagctattgagcgtccagaactacagaaggcactcactccgtctgagagaatcgttatggacatcattaagcgtcactttgacaccaagcgtgaacttatggaaaacccagcaatattcggtaacacaaaggctgtgagtatcttccctgagagtcgccacaaaggtacttacgttcctcacgtatatgaccgtcatgccaaggcgctgatgattcaacgctacggtgccgaaggtttgcaggaagggattgcccgctcatggatgaacagctacgtctccagacctgaggtcaaggccagagtcgatgagatgcttaaggaattacacggggtgaaggaagtaacaccagagatggtagagaagtacgctatggataaggcttatggtatctcccactcagaccagttcaccaacagttccataatagaagagaacattgagggcttagtaggtatcgagaataactcattccttgaggcacgtaacttgtttgattcggacctatccatcactatgccagacggacagcaattctcagtgaatgacctaagggacttcgatatgttccgcatcatgccagcgtatgaccgccgtgtcaatggtgacatcgccatcatggggtctactggtaaaaccactaaggaacttaaggatgagattttggctctcaaagcgaaagctgagggagacggtaagaagactggcgaggtacatgctttaatggataccgttaagattcttactggtcgtgctagacgcaatcaggacactgtgtgggaaacctcactgcgtgccatcaatgacctagggttcttcgctaagaacgcctacatgggtgctcagaacattacggagattgctgggatgattgtcactggtaacgttcgtgctctagggcatggtatcccaattctgcgtgatacactctacaagtctaaaccagtttcagctaaggaactcaaggaactccatgcgtctctgttcgggaaggaggtggaccagttgattcggcctaaacgtgctgacattgtgcagcgcctaagggaagcaactgataccggacctgccgtggcgaacatcgtagggaccttgaagtattcaacacaggaactggctgctcgctctccgtggactaagctactgaacggaaccactaactaccttctggatgctgcgcgtcaaggtatgcttggggatgttattagtgccaccctaacaggtaagactacccgctgggagaaagaaggcttccttcgtggtgcctccgtaactcctgagcagatggctggcatcaagtctctcatcaaggaacatatggtacgcggtgaggacgggaagtttaccgttaaggacaagcaagcgttctctatggacccacgggctatggacttatggagactggctgacaaggtagctgatgaggcaatgctgcgtccacataaggtgtccttacaggattcccatgcgttcggagcactaggtaagatggttatgcagtttaagtctttcactatcaagtcccttaactctaagttcctgcgaaccttctatgatggatacaagaacaaccgagcgattgacgctgcgctgagcatcatcacctctatgggtctcgctggtggtttctatgctatggctgcacacgtcaaagcatacgctctgcctaaggagaaacgtaaggagtacttggagcgtgcactggacccaaccatgattgcccacgctgcgttatctcgtagttctcaattgggtgctcctttggctatggttgacctagttggtggtgttttagggttcgagtcctccaagatggctcgctctacgattctacctaaggacaccgtgaaggaacgtgacccaaacaaaccgtacacctctagagaggtaatgggcgctatgggttcaaaccttctggaacagatgccttcggctggctttgtggctaacgtaggggctaccttaatgaatgctgctggcgtggtcaactcacctaataaagcaaccgagcaggacttcatgactggtcttatgaactccacaaaagagttagtaccgaacgacccactgacccaacagcttgtgttgaaaatctacgaggcgaacggtgttaacttgagggagcgtaggaaataa BBa_Z0272_sequence 1 taactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctg BBa_Z0127_sequence 1 atggctagcatgactggtggacagcaaatgggtactaaccaaggtaaaggtgtagttgctgctggagataaactggcgttgttcttgaaggtatttggcggtgaagtcctgactgcgttcgctcgtacctccgtgaccacttctcgccacatggtacgttccatctccagcggtaaatccgctcagttccctgttctgggtcgcactcaggcagcgtatctggctccgggcgagaacctcgacgataaacgtaaggacatcaaacacaccgagaaggtaatcaccattgacggtctcctgacggctgacgttctgatttatgatattgaggacgcgatgaaccactacgacgttcgctctgagtatacctctcagttgggtgaatctctggcgatggctgcggatggtgcggttctggctgagattgccggtctgtgtaacgtggaaagcaaatataatgagaacatcgagggcttaggtactgctaccgtaattgagaccactcagaacaaggccgcacttaccgaccaagttgcgctgggtaaggagattattgcggctctgactaaggctcgtgcggctctgaccaagaactatgttccggctgctgaccgtgtgttctactgtgacccagatagctactctgcgattctggcagcactgatgccgaacgcagcaaactacgctgctctgattgaccctgagaagggttctatccgcaacgttatgggctttgaggttgtagaagttccgcacctcaccgctggtggtgctggtaccgctcgtgagggcactactggtcagaagcacgtcttccctgccaataaaggtgagggtaatgtcaaggttgctaaggacaacgttatcggcctgttcatgcaccgctctgcggtaggtactgttaagctgcgtgacttggctctggagcgcgctcgccgtgctaacttccaagcggaccagattatcgctaagtacgcaatgggccacggtggtcttcgcccagaagctgctggtgcagtggttttcaaagtggagtaatgctgggggtggcctcaacggtcgctgctagtcccgaagaggcgagtgttacttcaacagaagaaaccttaacgccagcacaggaggccgcacgcacccgcgctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataacccggg BBa_Z0293_sequence 1 ggtcttcctgaccgactgatggctcaccgagggattcagcggtatgattgcatcacaccacttcatccctatagagtcaagtcctaaggtatacccataaagagcctctaatggtctatcctaaggtctatacctaaagataggccatcctatcagtgtcacctaaagagggtcttagagagggcctatggagttcctatagggtcctttaaaatataccataaaaatctgagtgactatctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccct BBa_Z0126_sequence 1 atggcagagagtaacgcagatgtatacgcaagttttggcgtgaactccgctgtgatgtctggtggttccgttgaggaacatgagcagaacatgctggctcttgatgttgctgcccgtgatggcgatgatgcaatcgagttagcgtcagacgaagtggaaacagaacgtgacctgtatgacaactctgacccgttcggtcaagaggatgacgaaggccgcattcaggttcgtatcggtgatggctctgagccgaccgatgtggacactggagaagaaggcgttgagggcaccgaaggttccgaagagtttaccccactgggcgagactccagaagaactggtagctgcctctgagcaacttggtgagcacgaagagggcttccaagagatgattaacattgctgctgagcgtggcatgagtgtcgagaccattgaggctatccagcgtgagtacgaggagaacgaagagttgtccgccgagtcctacgctaagctggctgaaattggctacacgaaggctttcattgactcgtatatccgtggtcaagaagctctggtggagcagtacgtaaacagtgtcattgagtacgctggtggtcgtgaacgttttgatgcactgtataaccaccttgagacgcacaaccctgaggctgcacagtcgctggataatgcgttgaccaatcgtgacttagcgaccgttaaggctatcatcaacttggctggtgagtctcgcgctaaggcgttcggtcgtaagccaactcgtagtgtgactaatcgtgctattccggctaaacctcaggctaccaagcgtgaaggctttgcggaccgtagcgagatgattaaagctatgagtgaccctcggtatcgcacagatgccaactatcgtcgtcaagtcgaacagaaagtaatcgattcgaacttctga BBa_Z0158_sequence 1 atggctatgtctaacatgacttacaacaacgttttcgaccacgcttacgaaatgctgaaagaaaacatccgttatgatgacatccgtgacactgatgacctgcacgatgctattcacatggctgccgataatgcagttccgcactactacgctgacatctttagcgtaatggcaagtgagggcattgaccttgagttcgaagactctggtctgatgcctgacaccaaggacgtaatccgcatcctgcaagcgcgtatctatgagcaattaacgattgacctctgggaagacgcagaagacttgctcaatgaatacttggaggaagtcgaggagtacgaggaggacgaagagtaagggccc BBa_Z0144_sequence 1 atgctagagttcctccgtaagctaatcccttgggttctcgctgggatgctattcgggttaggatggcatctagggtcagactcaatggacgctaaatggaaacaggaggtacacaatgagtacgttaagagagttgaggctgcgaagagcactcaaagagcaatcgatgcggtatctgctaagtatcaagaagaccttgccgcgctggaagggagcactgataggattatttctgatttgcgtagcgacaataagcggttgcgcgtcagagtcaaaactaccggaacctccgatggtcagtgtggattcgagcctgatggtcgagccgaacttgacgaccgagatgctaaacgtattctcgcagtgacccagaagggtgacgcatggattcgtgcgttacaggatactattcgtgaactgcaacgtaagtagcatgc BBa_Z0156_sequence 1 atgtgctgggcagcagctataccgattgcaataagtggcgctcaggctatcagtggtcagaacgctcaggccaaaatgattgccgctcagaccgctgctggtcgtcgtcaagctatggaaatcatgaggcagacgaacatccagaatgctgacctatcgttgcaagctcgaagtaaacttgaggaagcgtccgccgagttgacctcacagaacatgcagaaggtccaagctattgggtctatccgagcggctatcggagagagtatgcttgaaggttcctcaatggaccgcattaagcgagtcacagaaggacagttcattcgggaagccaatatggtaactgagaactatcgccgtgactaccaagcaatcttcgcacagcaacttggtggtactcaaagtgctgcaagtcagattgacgaaatctataagagcgaacagaaacagaagagtaagctacagatggttctggacccactggctatcatggggtcttccgctgcgagtgcttacgcatccggtgcgttcgactctaagtccacaactaaggcacctattgttgccgctaaaggaaccaagaccggcaggtaaccgcgg BBa_Z0128_sequence 1 atgcgaagttatgatatgaatgtagaaacagcggcagaattatcagctgtgaacgacattctggcgtctatcggtgaacctccggtatcaacgctggaaggtgacgctaacgcagatgcagcgaacgctcggcgtattctcaacaagattaaccgacagattcaatctcgtggatggacgttcaacattgaggaaggcataacgctactacctgatgtttactccaacctgattgtatacagtgacgactatttatccctaatgtctacttccggtcaatccatctacgttaaccgaggtggctatgtgtatgaccgaacgagtcaatcagaccgctttgactctggtattactgtgaacattattcgtctccgcgactacgatgagatgcctgagtgcttccgttactggattgtcaccaaggcttcccgtcagttcaacaaccgattctttggggcaccggaagtagagggtgtactccaagaagaggaagatgaggctagacgtctctgcatggagtatgagatggactacggtgggtacaatatgctggatggagatgcgttcacttctggtctactgactcgctaa BBa_Z0252_sequence 1 ataattaattgaactcactaaagggagaccacagc BBa_Z0108_sequence 1 atgcgtaacttcgaaaagatgaccaaacgttctaaccgtaatgctcgtgacttcgaggcaaccaaaggtcgcaagttgaataagactaagcgtgaccgctctcacaagcgtagctgggagggtcagtaa BBa_Z0132_sequence 1 atgagtaaaattgaatctgcccttcaagcggcacaaccgggactctctcggttacgtggtggtgctggaggtatgggctatcgtgcagcaaccactcaggccgaacagccaaggtcaagcctattggacaccattggtcggttcgctaaggctggtgccgatatgtataccgctaaggaacaacgagcacgagacctagctgatgaacgctctaacgagattatccgtaagctgacccctgagcaacgtcgagaagctctcaacaacgggacccttctgtatcaggatgacccatacgctatggaagcactccgagtcaagactggtcgtaacgctgcgtatcttgtggacgatgacgttatgcagaagataaaagagggtgtcttccgtactcgcgaagagatggaagagtatcgccatagtcgccttcaagagggcgctaaggtatacgctgagcagttcggcatcgaccctgaggacgttgattatcagcgtggtttcaacggggacattaccgagcgtaacatctcgctgtatggtgcgcatgataacttcttgagccagcaagctcagaagggcgctatcatgaacagccgagtggaactcaacggtgtccttcaagaccctgatatgctgcgtcgtccagactctgctgacttctttgagaagtatatcgacaacggtctggttactggcgcaatcccatctgatgctcaagccacacagcttataagccaagcgttcagtgacgcttctagccgtgctggtggtgctgacttcctgatgcgagtcggtgacaagaaggtaacacttaacggagccactacgacttaccgagagttgattggtgaggaacagtggaacgctctcatggtcacagcacaacgttctcagtttgagactgacgcgaagctgaacgagcagtatcgcttgaagattaactctgcgctgaaccaagaggacccaaggacagcttgggagatgcttcaaggtatcaaggctgaactagataaggtccaacctgatgagcagatgacaccacaacgtgagtggctaatctccgcacaggaacaagttcagaatcagatgaacgcatggacgaaagctcaggccaaggctctggacgattccatgaagtcaatgaacaaacttgacgtaatcgacaagcaattccagaagcgaatcaacggtgagtgggtctcaacggattttaaggatatgccagtcaacgagaacactggtgagttcaagcatagcgatatggttaactacgccaataagaagctcgctgagattgacagtatggacattccagacggtgccaaggatgctatgaagttgaagtaccttcaagcggactctaaggacggagcattccgtacagccatcggaaccatggtcactgacgctggtcaagagtggtctgccgctgtgattaacggtaagttaccagaacgaaccccagctatggatgctctgcgcagaatccgcaatgctgaccctcagttgattgctgcgctatacccagaccaagctgagctattcctgacgatggacatgatggacaagcagggtattgaccctcaggttattcttgatgccgaccgactgactgttaagcggtccaaagagcaacgctttgaggatgataaagcattcgagtctgcactgaatgcatctaaggctcctgagattgcccgtatgccagcgtcactgcgcgaatctgcacgtaagatttatgactccgttaagtatcgctcggggaacgaaagcatggctatggagcagatgaccaagttccttaaggaatctacctacacgttcactggtgatgatgttgacggtgataccgttggtgtgattcctaagaatatgatgcaggttaactctgacccgaaatcatgggagcaaggtcgggatattctggaggaagcacgtaagggaatcattgcgagcaacccttggataaccaataagcaactgaccatgtattctcaaggtgactccatttaccttatggacaccacaggtcaagtcagagtccgatacgacaaagagttactctcgaaggtctggagtgagaaccagaagaaactcgaagagaaagctcgtgagaaggctctggctgatgtgaacaagcgagcacctatagttgccgctacgaaggcccgtgaagctgctgctaaacgagtccgagagaaacgtaaacagactcctaagttcatctacggacgtaaggagtaa BBa_Z0160_sequence 1 atggctatgacaaagaaatttaaagtgtccttcgacgttaccgcaaagatgtcgtctgacgttcaggcaatcttagagaaagatatgctgcatctatgtaagcaggtcggctcaggtgcgattgtccccaatggtaaacagaaggaaatgattgtccagttcctgacacacggtatggaaggattgatgacattcgtagtacgtacatcatttcgtgaggccattaaggacatgcacgaagagtatgcagataaggactctttcaaacaatctcctgcaacagtacgggaggtgttctgatgtctgactacctgaaagtgctgcaagcaatcaaaagttgccctaagactttccagtccaactatgtacggaacaatgcgagcctcgtagcggaggccgcttcccgtggtcacatctcgtgcctgactactagtggacgtaacggtggcgcttgggaaatcactgcttccggtactcgctttctgaaacgaatgggcgggtgtgtctaa BBa_Z0116_sequence 1 atgagcaatgtaaataccggatccttaagcgtagataataagaagttttgggctaccgtagagtcctcggagcattccttcgaggttccaatctacgctgagaccctagacgaagctctggagttagccgaatggcaatacgttccggctggctttgaggttactcgtgtgcgtccttgtgtagcaccgaagtaagaattc BBa_Z0273_sequence 1 gtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcac BBa_Z0261_sequence 1 aatcaataggagaaatcaat BBa_Z0152_sequence 1 atggcgaaaaaaatatttacaagtgcgttaggaacagctgaaccttacgcttacatcgccaagccggactacggcaacgaagagcgtggctttgggaaccctcgtggtgtctataaagttgacctgactattcccaacaaagacccgcgctgccagcgtatggtcgatgaaatcgtgaagtgtcacgaagaggcttatgctgctgccgttgaggaatacgaagctaatccacctgctgtagctcgtggtaagaaaccgctgaaaccgtatgagggtgacatgccgttcttcgataacggtgacggtacgactacctttaagttcaaatgctacgcgtctttccaagacaagaagaccaaagagaccaagcacatcaatctggttgtggttgactcaaaaggtaagaagatggaagacgttccgattatcggtggtggctctaagctgaaagttaaatattctctggttccatacaagtggaacactgctgtaggtgcgagcgttaagctgcaactggaatccgtgatgctggtcgaactggctacctttggtggcggtgaagacgattgggctgacgaagttgaagagaacggctatgttgcctctggttctgccaaagcgagcaaaccacgcgacgaagaaagctgggacgaagacgacgaagagtccgaggaagcagacgaagacggggacttctaa BBa_Z0137_sequence 1 gtgagcacacagtccaatcgaaacgcattagttgtagctcaactgaaaggagacttcgtggcgttcctattcgtcttatggaaggcgctaaacctaccggtgcccactaagtgtcagattgacatggctaaggtgctggcgaatggagacaacaagaagttcatcttacaggctttccgtggtatcggtaagtcgttcatcacatgtgcgttcgttgtgtggtccttatggagagaccctcagttgaagatacttatcgtatcagcctctaaggagcgtgcagacgctaactccatctttattaagaacatcattgacctgctgccattcctatctgagttaaagccaagacccggacagcgtgactcggtaatcagctttgatgtaggcccagccaatcctgaccactctcctagtgtgaaatcagtaggtatcactggtcagttaactggtagccgtgctgacattatcattgcggatgacgttgagattccgtctaacagcgcaactatgggtgcccgtgagaagctatggactctggttcaggagttcgctgcgttacttaaaccgctgccttcctctcgcgttatctaccttggtacacctcagacagagatgactctctataaggaacttgaggataaccgtgggtacacaaccattatctggcctgctctgtacccaaggacacgtgaagagaacctctattactcacagcgtcttgctcctatgttacgcgctgagtacgatgagaaccctgaggcacttgctgggactccaacagacccagtgcgctttgaccgtgatgacctgcgcgagcgtgagttggaatacggtaaggctggctttacgctacagttcatgcttaaccctaaccttagtgatgccgagaagtacccgctgaggcttcgtgacgctatcgtagcggccttagacttagagaaggccccaatgcattaccagtggcttccgaaccgtcagaacatcattgaggaccttcctaacgttggccttaagggtgatgacctgcatacgtaccacgattgttccaacaactcaggtcagtaccaacagaagattctggtcattgaccctagtggtcgcggtaaggacgaaacaggttacgctgtgctgtacacactgaacggttacatctaccttatggaagctggaggtttccgtgatggctactccgataagacccttgagttactcgctaagaaggcaaagcaatggggagtccagacggttgtctacgagagtaacttcggtgacggtatgttcggtaaggtattcagtcctatccttcttaaacaccacaactgtgcgatggaagagattcgtgcccgtggtatgaaagagatgcgtatttgcgatacccttgagccagtcatgcagactcaccgccttgtaattcgtgatgaggtcattagggccgactaccagtccgctcgtgacgtagacggtaagcatgacgttaagtactcgttgttctaccagatgacccgtatcactcgtgagaaaggcgctctggctcatgatgaccgattggatgcccttgcgttaggcattgagtatctccgtgagtccatgcagttggattccgttaaggtcgagggtgaagtacttgctgacttccttgaggaacacatgatgcgtcctacggttgctgctacgcatatcattgagatgtctgtgggaggagttgatgtgtactctgaggacgatgagggttacggtacgtctttcattgagtggtga BBa_Z0120_sequence 1 atggataatagtcatgatagcgatagcgtttttttgtatcacattccttgtgacaactgtgggagtagtgacgggaactcgctgttctctgacggacacacgttctgctacgtatgcgagaagtggactgctggtaatgaagacactaaagagagggcttcaaaacggaaaccctcaggaggtaaaccaatgacttacaacgtgtggaacttcggggaatccaatggacgctactccgcgttaactgcgagaggaatctccaaggaaacctgtcagaaggctggctactggattgccaaagtagacggtgtgatgtaccaagtggctgactatcgggaccagaacggcaacattgtgagtcagaaggttcgagataaagataagaactttaagaccactggtagtcacaagagtgacgctctgttcgggaagcacttgtggaatggtggtaagaagattgtcgttacagaaggtgaaatcgacatgcttaccgtgatggaacttcaagactgtaagtatcctgtagtgtcgttgggtcacggtgcctctgccgctaagaagacatgcgctgccaactacgaatacttcgatcagttcgaacagattatcctgatgttcgatatggacgaagcagggcgcaaagcagtcgaagaggctgcacaggttctacctgctggtaaggtacgagtggcagttcttccgtgtaaggatgcaaacgagtgtcacctaaatggtcacgaccgtgaaatcatggagcaagtgtggaatgctggtccttggattcctgatggtgtggtatcggctctttcgttacgtgaacgaatccgtgagcacctatcgtccgaggaatcagtaggtttacttttcagtggctgcactggtatcaacgataagaccttaggtgcccgtggtggtgaagtcattatggtcacttccggttccggtatgggtaagtcaacgttcgtccgtcaacaagctctacaatggggcacagcgatgggcaagaaggtaggcttagcgatgcttgaggagtccgttgaggagaccgctgaggaccttataggtctacacaaccgtgttcgtctgcgtcagtctgactctctgaaacgtgaaatcattgagaacggtaagttcgaccaatggttcgatgaactgttcggcaacgatacgttccatctatatgactcattcgccgaggctgagacggatagactgctcgctaagctggcctacatgcgctcaggcttgggctgtgacgtaatcattctagaccacatctcaatcgtcgtatccgcttctggtgaatccgatgagcgtaagatgattgacaacctgatgaccaagctcaaagggttcgctaagtcaactggggtggtgctggtcgtaatttgtcaccttaagaacccagacaaaggtaaagcacacgaggaaggtcgccccgtttctattactgacctacgtggttctggcgcactacgccaactatctgatactattattgcccttgagcgtaatcagcaaggcgatatgcctaaccttgtcctcgttcgtattctcaagtgccgctttactggtgatactggtatcgctggctacatggaatacaacaaggaaaccggatggcttgaaccatcaagttactcaggggaagaagagtcacactcagagtcaacagactggtccaacgacactgacttctaagccggc BBa_Z0154_sequence 1 atggcacttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcgactggctggtcttccaagctatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggcccgtcagattcttgaggattccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgttaactggcgtaaagaactggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgctctctttgagcgcgaagagttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgccttcggtgctcgtaaggctgtaatcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcctgactcagaccgaagagtccgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggggtgataccgccgaggacttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagaggttactaaatgggttaaacgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaagaggatattatcaagcagggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggaaatttacctctggagaccgtagctcgag BBa_Z0138_sequence 1 atgttcaaactgattaagaagttaggccaactgctggttcgtatgtacaacgtggaagccaagcgactgaacgatgaggctcgtaaagaggccacacagtcacgcgctctggcgattcgctccaacgaactggctgacagtgcatccactaaagttaccgaggctgcccgtgtggcaaaccaagctcaacagctttccaaattctttgagtaa BBa_Z0124_sequence 1 atgggtaagaaagttaagaaggccgtgaagaaagtcaccaagtccgttaagaaagtcgttaaggaaggggctcgtccggttaaacaggttgctggcggtctagctggtctggctggtggtactggtgaagcacagatggtggaagtaccacaagctgccgcacagattgttgacgtacctgagaaagaggtttccactgaggacgaagcacagacagaaagcggacgcaagaaagctcgtgctggcggtaagaaatccttgagtgtagcccgtagctccggtggcggtatcaacatctaaggtcacc BBa_Z0139_sequence 1 atgtctaacgtagctgaaactatccgtctatccgatacagctgaccagtggaaccgtcgagtccacatcaacgttcgcaacggtaaggcgactatggtttaccgctggaaggactctaagtcctctaagaatcacactcagcgtatgacgttgacagatgagcaagcactgcgtctggtcaatgcgcttaccaaagctgccgtgacagcaattcatgaagctggtcgcgtcaatgaagctatggctatcctcgacaagattgataactaa BBa_Z0301_sequence 1 atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa BBa_Z0115_sequence 1 atgggacgtttatatagtggtaatctggcagcattcaaggcagcaacaaacaagctgttccagttagacttagcggtcatttatgatgactggtatgatgcctatacaagaaaagattgcatacggttacgtattgaggacaggagtggaaacctgattgatactagcaccttctaccaccacgacgaggacgttctgttcaatatgtgtactgattggttgaaccatatgtatgaccagttgaaggactggaagtaa BBa_Z0271_sequence 1 cacactggctcaccttcgggtgggcctttctgcgttt BBa_Z0151_sequence 1 atgggactgttagatggtgaagcctgggaaaaagaaaacccgccagtacaagcaactgggtgtatagcttgcttagagaaagatgaccgttatccacacacctgtaacaaaggagctaacgatatgaccgaacgtgaacaagagatgatcattaagttgatagacaataatgaaggtcgcccagatgatttgaatggctgcggtattctctgctccaatgtcccttgccacctctgccccgcaaataacgatcaaaagataaccttaggtgaaatccgagcgatggacccacgtaaaccacatctgaataaacctgaggtaactcctacagatgaccagccttccgctgagacaatcgaaggtgtcactaagccttcccactacatgctgtttgacgacattgaggctatcgaagtgattgctcgttcaatgaccgttgagcagttcaagggatactgcttcggtaacatcttaaagtacagactacgtgctggtaagaagtcagagttagcgtacttagagaaagacctagcgaaagcagacttctataaagaactctttgagaaacataaagataaatgttacgcataa BBa_Z0107_sequence 1 atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggtagatacatatgagtcttgtgacgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaatccgga BBa_Z0291_sequence 1 taacgccaaatcaatacgactcactatagagggacaaactcaaggtcattcgcaagagtggcctttatgattgaccttcttccggttaatacgactcactataggagaaccttaaggtttaactttaagacccttaagtgttaattagagatttaaatta BBa_Z0262_sequence 1 ttaaagaggagaaatactag BBa_Z0292_sequence 1 tctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccctatctgttacagtctcctaaagtatcctcctaaagtcacctcctaacgtccatcctaaagccaacacctaaagcctacacctaaagacccatcaagtcaacgcctatcttaaagtttaaacataaagaccagacctaaagaccagacctaaagacactacataaagaccagacctaaagacgccttgttgttagccataaagtgataacctttaatca BBa_Z0125_sequence 1 atggctgagaaacgaacaggacttgcggaggatggcgcaaagtctgtctatgagcgtttaaagaacgaccgtgctccctatgagacacgcgctcagaattgcgctcaatataccatcccatcattgttccctaaggactccgataacgcctctacagattatcaaactccgtggcaagccgtgggcgctcgtggtctgaacaatctagcctctaagctcatgctggctctattccctatgcagacttggatgcgacttactatatctgaatatgaagcaaagcagttactgagcgaccccgatggactcgctaaggtcgatgagggcctctcgatggtagagcgtatcatcatgaactacattgagtctaacagttaccgcgtgactctctttgaggctctcaaacagttagtcgtagctggtaacgtcctgctgtacctaccggaaccggaagggtcaaactataatcccatgaagctgtaccgattgtcttcttatgtggtccaacgagacgcattcggcaacgttctgcaaatggtgactcgtgaccagatagcttttggtgctctccctgaggacatccgtaaggctgtagaaggtcaaggtggtgagaagaaagctgatgagacaatcgacgtgtacactcacatctatctggatgaggactcaggtgaatacctccgatacgaagaggtcgagggtatggaagtccaaggctccgatgggacttatcctaaagaggcttgcccatacatcccgattcggatggtcagactagatggtgaatcctacggtcgttcgtacattgaggaatacttaggtgacttacggtcccttgaaaatctccaagaggctatcgtcaagatgtccatgattagctctaaggttatcggcttagtgaatcctgctggtatcacccagccacgccgactgaccaaagctcagactggtgacttcgttactggtcgtccagaagacatctcgttcctccaactggagaagcaagcagactttactgtagctaaagccgtaagtgacgctatcgaggctcgcctttcgtttgcctttatgttgaactctgcggttcagcgtacaggtgaacgtgtgaccgccgaagagattcggtatgtagcttctgaacttgaagatactttaggtggtgtctactctatcctttctcaagaattacaattgcctctggtacgagtgctcttgaagcaactacaagccacgcaacagattcctgagttacctaaggaagccgtagagccaaccattagtacaggtctggaagcaattggtcgaggacaagaccttgataagctggagcggtgtgtcactgcgtgggctgcactggcacctatgcgggacgaccctgatattaaccttgcgatgattaagttacgtattgccaacgctatcggtattgacacttctggtattctactcaccgaagaacagaagcaacagaagatggcccaacagtctatgcaaatgggtatggataatggtgctgctgcgctggctcaaggtatggctgcacaagctacagcttcacctgaggctatggctgctgccgctgattccgtaggtttacagccgggaatttaagtcgac BBa_Z0123_sequence 1 atgtgtttctcaccgaaaattaaaactccgaagatggataccaatcagattcgagccgttgagccagcgcctctgacccaagaagtgtcaagcgtggagttcggtgggtcttctgatgagacggataccgagggcaccgaagtgtctggacgcaaaggcctcaaggtcgaacgtgatgattccgtagcgaagtctaaagccagcggcaatggctccgctcgtatgaaatcttccatccgtaagtccgcatttggaggtaagaagtga BBa_Z0136_sequence 1 atggaaaaggataagagccttattacattcttagagatgttggacactgcgatggctcagcgtatgcttgcggacctttcggaccatgagcgtcgctctccgcaactctataatgctattaacaaactgttagaccgccacaagttccagattggtaagttgcagccggatgttcacatcttaggtggccttgctggtgctcttgaagagtacaaagagaaagtcggtgataacggtcttacggatgatgatatttacacattacagtgaagctt BBa_Z0129_sequence 1 atggcactgatatcccaaagcattaaaaatttaaaaggcggtatcagccaacagcctgacatccttcgttatccagaccaagggtcacgccaagttaacggttggtcttcggagaccgagggcctccaaaagcgtccacctcttgttttcttaaatacacttggagacaacggtgcgttaggtcaagctccgtacatccacctgattaaccgagatgagcacgaacagtattacgctgtgttcactggtagcggaatccgagtgttcgacctttctggtaacgagaagcaagttaggtatcctaacggttccaactacatcaagaccgctaatccacgtaacgacctgcgaatggttactgtagcagactatacgttcatcgtgaaccgtaacgttgttgcacagaagaacacaaagtctgtcaacttaccgaattacaaccctaatcaagacggattgattaacgttcgtggtggtcagtatggtagggaactaattgtacacattaacggtaaagacgttgcgaagtataagataccagatggtagtcaacctgaacacgtaaacaatacggatgcccaatggttagctgaagagttagccaagcagatgcgcactaacttgtctgattggactgtaaatgtagggcaagggttcatccatgtgaccgcacctagtggtcaacagattgactccttcacgactaaagatggctacgcagaccagttgattaaccctgtgacccactacgctcagtcgttctctaagctgccacctaatgctcctaacggctacatggtgaaaatcgtaggggacgcctctaagtctgccgaccagtattacgttcggtatgacgctgagcggaaagtttggactgagactttaggttggaacactgaggaccaagttctatgggaaaccatgccacacgctcttgtgcgagccgctgacggtaatttcgacttcaagtggcttgagtggtctcctaagtcttgtggtgacgttgacaccaacccttggccttcttttgttggttcaagtattaacgatgtgttcttcttccgtaaccgcttaggattccttagtggggagaacatcatattgagtcgtacagccaaatacttcaacttctaccctgcgtccattgcgaaccttagtgatgacgaccctatagacgtagctgtgagtaccaaccgaatagcaatccttaagtacgccgttccgttctcagaagagttactcatctggtccgatgaagcacaattcgtcctgactgcctcgggtactctcacatctaagtcggttgagttgaacctaacgacccagtttgacgtacaggaccgagcgagaccttttgggattgggcgtaatgtctactttgctagtccgaggtccagcttcacgtccatccacaggtactacgctgtgcaggatgtcagttccgttaagaatgctgaggacattacatcacacgttcctaactacatccctaatggtgtgttcagtatttgcggaagtggtacggaaaacttctgttcggtactatctcacggggaccctagtaaaatcttcatgtacaaattcctgtacctgaacgaagagttaaggcaacagtcgtggtctcattgggactttggggaaaacgtacaggttctagcttgtcagagtatcagctcagatatgtatgtgattcttcgcaatgagttcaatacgttcctagctagaatctctttcactaagaacgccattgacttacagggagaaccctatcgtgcctttatggacatgaagattcgatacacgattcctagtggaacatacaacgatgacacattcactacctctattcatattccaacaatttatggtgcaaacttcgggaggggcaaaatcactgtattggagcctgatggtaagataaccgtgtttgagcaacctacggctgggtggaatagcgacccttggctgagactcagcggtaacttggagggacgcatggtgtacattgggttcaacattaacttcgtatatgagttctctaagttcctcatcaagcagactgccgacgacgggtctacctccacggaagacattgggcgcttacagttacgccgagcgtgggttaactacgagaactctggtacgtttgacatttatgttgagaaccaatcgtctaactggaagtacacaatggctggtgcccgattaggctctaacactctgagggctgggagactgaacttagggaccggacaatatcgattccctgtggttggtaacgccaagttcaacactgtatacatcttgtcagatgagactacccctctgaacatcattgggtgtggctgggaaggtaactacttacggagaagttccggtatttaattaa BBa_Z0505_sequence 1 tctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccctatctgttacagtctcctaaagtatcctcctaaagtcacctcctaacgtccatcctaaagccaacacctaaagcctacacctaaagacccatcaagtcaacgcctatcttaaagtttaaacataaagaccagacctaaagaccagacctaaagacactacataaagaccagacctaaagacgccttgttgttagccataaagtgataacctttaatcaagcaccgaagtaatacgactcactattagggaagacaacttaaagagacttaaaagattaatttaaaatttatcaaaaagagtattgacttaaagtctaacctataggatacttacagccatcgagagggacacggcgaatagccatcccaatcgacaccggggtcaaccggataagtagacagcctgataagtcgcacgaaaaacaggtattgacaacatgaagtaacatgcagtaagatacaaatcgctaggtaacactagcagcgtcaaccgggcgcacagtgccttctaggtgacttaagcgcaccacggcacataaggtgaaacaaaacggttgacaacatgaagtaaacacggtacgatgtaccacatgaaacgacagtgagtcaccacactgaaaggtgatgcggtctaacgaaacctgacctaagacgctctttaacaatctggtaaatagctcttgagctcgtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcacaatcaataggagaaatcaatatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataaaatcaataggagaaatcaatatggctatgtctaacatgacttacaacaacgttttcgaccacgcttacgaaatgctgaaagaaaacatccgttatgatgacatccgtgacactgatgacctgcacgatgctattcacatggctgccgataatgcagttccgcactactacgctgacatctttagcgtaatggcaagtgagggcattgaccttgagttcgaagactctggtctgatgcctgacaccaaggacgtaatccgcatcctgcaagcgcgtatctatgagcaattaacgattgacctctgggaagacgcagaagacttgctcaatgaatacttggaggaagtcgaggagtacgaggaggacgaagagtaagggcccgtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcacttaaagaggagaaatactagatgaacattaccgacatcatgaacgctatcgacgcaatcaaagcactgccaatctgtgaacttgacaagcgtcaaggtatgcttatcgacttactggtcgagatggtcaacagcgagacgtgtgatggcgagctaaccgaactaaatcaggcacttgagcatcaagattggtggactaccttgaagtgtctcacggctgacgcagggttcaagatgctcggtaatggtcacttctcggctgcttatagtcacccgctgctacctaacagagtgattaaggtgggctttaagaaagaggattcaggcgcagcctataccgcattctgccgcatgtatcagggtcgtcctggtatccctaacgtctacgatgtacagcgccacgctggatgctatacggtggtacttgacgcacttaaggattgcgagcgtttcaacaatgatgcccattataaatacgctgagattgcaagcgacatcattgattgcaattcggatgagcatgatgagttaactggatgggatggtgagtttgttgaaacttgtaaactaatccgcaagttctttgagggcatcgcctcattcgacatgcatagcgggaacatcatgttctcaaatggagacgtaccatacatcaccgacccggtatcattctcgcagaagaaagacggtggcgcattcagcatcgaccctgaggaactcatcaaggaagtcgaggaagtcgcacgacagaaagaaattgaccgcgctaaggcccgtaaagaacgtcacgaggggcgcttagaggcacgcagattcaaacgtcgcaaccgcaaggcacgtaaagcacacaaagctaagcgcgaaagaatgcttgctgcgtggcgatgggctgaacgtcaagaacggcgtaaccatgaggtagctgtagatgtactaggaagaaccaataacgctatgctctgggtcaacatgttctctggggactttaaggcgcttgaggaacgaatcgcgctgcactggcgtaatgctgaccggatggctatcgctaatggtcttacgctcaacattgataagcaacttgacgcaatgttaatgggctgacgtacggtgcatgactagcggataactcaagggtatcgcaaggtgccctttatgatattcactaataactgcacttaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttca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gtaggtatcactggtcagttaactggtagccgtgctgacattatcattgcggatgacgttgagattccgtctaacagcgcaactatgggtgcccgtgagaagctatggactctggttcaggagttcgctgcgttacttaaaccgctgccttcctctcgcgttatctaccttggtacacctcagacagagatgactctctataaggaacttgaggataaccgtgggtacacaaccattatctggcctgctctgtacccaaggacacgtgaagagaacctctattactcacagcgtcttgctcctatgttacgcgctgagtacgatgagaaccctgaggcacttgctgggactccaacagacccagtgcgctttgaccgtgatgacctgcgcgagcgtgagttggaatacggtaaggctggctttacgctacagttcatgcttaaccctaaccttagtgatgccgagaagtacccgctgaggcttcgtgacgctatcgtagcggccttagacttagagaaggccccaatgcattaccagtggcttccgaaccgtcagaacatcattgaggaccttcctaacgttggccttaagggtgatgacctgcatacgtaccacgattgttccaacaactcaggtcagtaccaacagaagattctggtcattgaccctagtggtcgcggtaaggacgaaacaggttacgctgtgctgtacacactgaacggttacatctaccttatggaagctggaggtttccgtgatggctactccgataagacccttgagttactcgctaagaaggcaaagcaatggggagtccagacggttgtctacgagagtaacttcggtgacggtatgttcggtaaggtattcagtcctatccttcttaaacaccacaactgtgcgatggaagagattcgtgcccgtggtatgaaagagatgcgtatttgcgatacccttgagccagtcatgcagactcaccgccttgtaattcgtgatgaggtcattagggccgactaccagtccgctcgtgacgtagacggtaagcatgacgttaagtactcgttgttctaccagatgacccgtatcactcgtgagaaaggcgctctggctcatgatgaccgattggatgcccttgcgttaggcattgagtatctccgtgagtccatgcagttggattccgttaaggtcgagggtgaagtacttgctgacttccttgaggaacacatgatgcgtcctacggttgctgctacgcatatcattgagatgtctgtgggaggagttgatgtgtactctgaggacgatgagggttacggtacgtctttcattgagtggtgagaaattaatacgactcactatagggagaccacaacaatcaataggagaaatcaatatgttccgcttattgttgaacctactgcggcatagagtcacctaccgatttcttgtggtactttgtgctgcccttgggtacgcatctcttactggagacctcagttcactggagtctgtcgtttgctctatactcacttgtagcgattagggtcttcctgaccgactgatggctcaccgagggattcagcggtatgattgcatcacaccacttcatccctatagagtcaagtcctaaggtatacccataaagagcctctaatggtctatcctaaggtctatacctaaagataggccatcctatcagtgtcacctaaagagggtcttagagagggcctatggagttcctatagggtcctttaaaatataccataaaaatctgagtgactatctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccct BBa_Z0134_sequence 1 atggctaacgtaattaaaaccgttttgacttaccagttagatggctccaatcgtgattttaatatcccgtttgagtatctagcccgtaagttcgtagtggtaactcttattggtgtagaccgaaaggtccttacgattaatacagactatcgctttgctacacgtactactatctctctgacaaaggcttggggtccagccgatggctacacgaccatcgagttacgtcgagtaacctccactaccgaccgattggttgactttacggatggttcaatcctccgcgcgtatgaccttaacgtcgctcagattcaaacgatgcacgtagcggaagaggcccgtgacctcactacggatactatcggtgtcaataacgatggtcacttggatgctcgtggtcgtcgaattgtgaacctagcgaacgccgtggatgaccgcgatgctgttccgtttggtcaactaaagaccatgaaccagaactcatggcaagcacgtaatgaagccttacagttccgtaatgaggctgagactttcagaaaccaagcggagggctttaagaacgagtccagtaccaacgctacgaacacaaagcagtggcgcgatgagaccaagggtttccgagacgaagccaagcggttcaagaatacggctggtcaatacgctacatctgctgggaactctgcttccgctgcgcatcaatctgaggtaaacgctgagaactctgccacagcatccgctaactctgctcatttggcagaacagcaagcagaccgtgcggaacgtgaggcagacaagctggaaaattacaatggattggctggtgcaattgataaggtagatggaaccaatgtgtactggaaaggaaatattcacgctaacgggcgcctttacatgaccacaaacggttttgactgtggccagtatcaacagttctttggtggtgtcactaatcgttactctgtcatggagtggggagatgagaacggatggctgatgtatgttcaacgtagagagtggacaacagcgataggcggtaacatccagttagtagtaaacggacagatcatcacccaaggtggagccatgaccggtcagctaaaattgcagaatgggcatgttcttcaattagagtccgcatccgacaaggcgcactatattctatctaaagatggtaacaggaataactggtacattggtagagggtcagataacaacaatgactgtaccttccactcctatgtacatggtacgaccttaacactcaagcaggactatgcagtagttaacaaacacttccacgtaggtcaggccgttgtggccactgatggtaatattcaaggtactaagtggggaggtaaatggctggatgcttacctacgtgacagcttcgttgcgaagtccaaggcgtggactcaggtgtggtctggtagtgctggcggtggggtaagtgtgactgtttcacaggatctccgcttccgcaatatctggattaagtgtgccaacaactcttggaacttcttccgtactggccccgatggaatctacttcatagcctctgatggtggatggttacgattccaaatacactccaacggtctcggattcaagaatattgcagacagtcgttcagtacctaatgcaatcatggtggagaacgagtaacgatcg BBa_Z0153_sequence 1 atggcaggttacggcgctaaaggaatccgaaaggttggagcgtttcgctctggcctagaggacaaggtttcaaagcagttggaatcaaaaggtattaaattcgagtatgaagagtggaaagtgccttatgtaattccggcgagcaatcacacttacactccagacttcttacttccaaacggtatattcgttgagacaaagggtctgtgggaaagcgatgatagaaagaagcacttattaattagggagcagcaccccgagctagacatccgtattgtcttctcaagctcacgtactaagttatacaaaggttctccaacgtcttatggagagttctgcgaaaagcatggtattaagttcgctgataaactgatacctgctgagtggataaaggaacccaagaaggaggtcccctttgatagattaaaacgcaagggggggaagaaataa BBa_Z0109_sequence 1 atgatgaacattaagactaacccgtttaaagccgtgtctttcgtagagtctgccattaagaaggctctggataacgctgggtatcttatcgctgaaatcaagtacgatggtgtacgcgggaacatctgcgtagacaatactgctaacagttactggctctctcgtgtatctaaaacgattccggcactggagcacttaaacgggtttgatgttcgctggaagcgtctactgaacgatgaccgttgcttctacaaagatggctttatgcttgatggggaactcatggtcaagggcgtagactttaacacagggtccggcctactgcgtaccaaatggactgacacgaagaaccaagagttccatgaagagttattcgttgaaccaatccgtaagaaagataaagttccctttaagctgcacactggacaccttcacataaaactgtacgctatcctcccgctgcacatcgtggagtctggagaagactgtgatgtcatgacgttgctcatgcaggaacacgttaagaacatgctgcctctgctacaggaatacttccctgaaatcgaatggcaagcggctgaatcttacgaggtctacgatatggtagaactacagcaactgtacgagcagaagcgagcagaaggccatgagggtctcattgtgaaagacccgatgtgtatctataagcgcggtaagaaatctggctggtggaaaatgaaacctgagaacgaagctgacggtatcattcagggtctggtatggggtacaaaaggtctggctaatgaaggtaaagtgattggttttgaggtgcttcttgagagtggtcgtttagttaacgccacgaatatctctcgcgccttaatggatgagttcactgagacagtaaaagaggccaccctaagtcaatggggattctttagcccatacggtattggcgacaacgatgcttgtactattaacccttacgatggctgggcgtgtcaaattagctacatggaggaaacacctgatggctctttgcggcacccatcgttcgtaatgttccgtggcaccgaggacaaccctcaagagaaaatgtaacggccg BBa_Z0253_sequence 1 agcaccgaagtaatacgactcactattagggaaga BBa_Z0157_sequence 1 gtgctatcattagactttaacaacgaattgattaaggctgctccaattgttgggacgggtgtagcagatgttagtgctcgactgttctttgggttaagccttaacgaatggttctacgttgctgctatcgcctacacagtggttcagattggtgccaaggtagtcgataagatgattgactggaagaaagccaataaagaatga BBa_Z0121_sequence 1 atgatcgtaagtgatatagaagcgaatgcattattagaaagcgtcactaagttccactgcggggttatctacgactactccaccgctgagtacgtaagctaccgtccgagtgacttcggtgcgtatctggatgcgctggaagccgaggttgcacgaggcggtcttattgtgttccacaacggtcacaagtatgacgttcctgcattgaccaaactggcaaagttgcaattgaaccgagagttccaccttcctcgtgagaactgtattgacacccttgtgttgtcacgtttgattcattccaacctcaaggacaccgatatgggtcttctgcgttccggcaagttgcccggaaaacgctttgggtctcacgctttggaggcgtggggttatcgcttaggcgagatgaagggtgaatacaaagacgactttaagcgtatgcttgaagagcagggtgaagaatacgttgacggaatggagtggtggaacttcaacgaagagatgatggactataacgttcaggacgttgtggtaactaaagctctccttgagaagctactctctgacaaacattacttccctcctgagattgactttacggacgtaggatacactacgttctggtcagaatcccttgaggccgttgacattgaacatcgtgctgcatggctgctcgctaaacaagagcgcaacgggttcccgtttgacacaaaagcaatcgaagagttgtacgtagagttagctgctcgccgctctgagttgctccgtaaattgaccgaaacgttcggctcgtggtatcagcctaaaggtggcactgagatgttctgccatccgcgaacaggtaagccactacctaaataccctcgcattaagacacctaaagttggtggtatctttaagaagcctaagaacaaggcacagcgagaaggccgtgagccttgcgaacttgatacccgcgagtacgttgctggtgctccttacaccccagttgaacatgttgtgtttaacccttcgtctcgtgaccacattcagaagaaactccaagaggctgggtgggtcccgaccaagtacaccgataagggtgctcctgtggtggacgatgaggtactcgaaggagtacgtgtagatgaccctgagaagcaagccgctatcgacctcattaaagagtacttgatgattcagaagcgaatcggacagtctgctgagggagacaaagcatggcttcgttatgttgctgaggatggtaagattcatggttctgttaaccctaatggagcagttacgggtcgtgcgacccatgcgttcccaaaccttgcgcaaattccgggtgtacgttctccttatggagagcagtgtcgcgctgcttttggcgctgagcaccatttggatgggataactggtaagccttgggttcaggctggcatcgacgcatccggtcttgagctacgctgcttggctcacttcatggctcgctttgataacggcgagtacgctcacgagattcttaacggcgacatccacactaagaaccagatagctgctgaactacctacccgagataacgctaagacgttcatctatgggttcctctatggtgctggtgatgagaagattggacagattgttggtgctggtaaagagcgcggtaaggaactcaagaagaaattccttgagaacacccccgcgattgcagcactccgcgagtctatccaacagacacttgtcgagtcctctcaatgggtagctggtgagcaacaagtcaagtggaaacgccgctggattaaaggtctggatggtcgtaaggtacacgttcgtagtcctcacgctgccttgaataccctactgcaatctgctggtgctctcatctgcaaactgtggattatcaagaccgaagagatgctcgtagagaaaggcttgaagcatggctgggatggggactttgcgtacatggcatgggtacatgatgaaatccaagtaggctgccgtaccgaagagattgctcaggtggtcattgagaccgcacaagaagcgatgcgctgggttggagaccactggaacttccggtgtcttctggataccgaaggtaagatgggtcctaattgggcgatttgccactgactgcag BBa_Z0119_sequence 1 atggctcgtgtacagtttaaacaacgtgaatctactgacgcaatctttgttcactgctcggctaccaagccaagtcagaatgttggtgtccgtgagattcgccagtggcacaaagagcagggctggctcgatgtgggataccacttcatcatcaagcgagacggtactgtggaggcaggacgagatgagatggctgtaggctctcacgctaagggttacaaccacaactctatcggcgtctgccttgttggtggtatcgacgataaaggtaagttcgacgctaactttacgccagcccaaatgcaatcccttcgctcactgcttgtcacactgctggctaagtacgaaggcgctgtgcttcgcgcccatcatgaggtggcgccgaaggcttgcccttcgttcgaccttaagcgttggtgggagaagaacgaactggtcacttctgaccgtggataagatct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z