//binding/cellulose
type: Collection
persistent identity: binding cellulose
display id: binding_cellulose
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1321121
member: BBa_K1321340
member: BBa_K1478001
member: BBa_K1321366
member: CBDcex
member: BBa_K1321105
member: BBa_K1321112
member: BBa_K1499004
member: BBa_K1933102
member: BBa_K1321090
member: BBa_K1321093
member: BBa_K1321114
member: BBa_K1321154
member: BBa_K1321352
member: BBa_K1321343
member: BBa_K1321003
member: BBa_K1321115
member: BBa_K1321356
member: BBa_K1321110
member: BBa_K1321351
member: BBa_K1979001
member: BBa_K1321339
member: CBDclos(T7
member: BBa_K1321002
member: BBa_K1321092
member: BBa_K1321122
member: BBa_K1321357
member: BBa_K1321152
member: BBa_K863113
member: BBa_K1321151
member: BBa_K1321014
member: BBa_K1933103
member: BBa_K1321104
member: BBa_K1321091
member: BBa_K1321345
member: BBa_K1321094
member: BBa_K1321353
member: CBDclos(J6
member: BBa_K1321120
member: BBa_K1321346
member: BBa_K1321344
member: BBa_K1933101
member: CBDcex(T7)
member: BBa_K1321111
member: BBa_K1321097
member: BBa_K1933100
member: CBDclos
member: BBa_K1321342
member: BBa_K1321348
member: BBa_K1321096
member: BBa_K1321101
member: CBDcex(J61
member: BBa_K863110
member: BBa_K1321100
member: BBa_K1321095
member: BBa_K1321341
member: BBa_K1321349

BBa_K1933100
type: ComponentDefinition
persistent identity: BBa K1933100
display id: BBa_K1933100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDcex fused to INPNC with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-17T11:35:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: true
mutable description: CBDcex fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDcex) fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: Promoter and RBS: The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321003
component: BBa_K1933004
component: BBa_K1933011
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1933011
sequence: https://synbiohub.org/public/igem/BBa_K1933100_sequence/1

BBa_K1321003
type: Component
persistent identity: component2516443
display id: component2516443
version: 1
top level: BBa_K1933100
access: public
definition: BBa_K1321003

BBa_K1933004
type: Component
persistent identity: component2516438
display id: component2516438
version: 1
top level: BBa_K1933100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1933011
type: Component
persistent identity: component2516442
display id: component2516442
version: 1
top level: BBa_K1933100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933011/1

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2516438
display id: annotation2516438
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516438/range2516438/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516438/range2516438/1
type: Range
persistent identity: range2516438
display id: range2516438
version: 1
direction: direction/reverse
top level: BBa_K1933100
start: 1
end: 60
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2516443
display id: annotation2516443
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516443/range2516443/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516443/range2516443/1
type: Range
persistent identity: range2516443
display id: range2516443
version: 1
direction: direction/reverse
top level: BBa_K1933100
start: 1036
end: 1362
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2516442
display id: annotation2516442
version: 1
top level: BBa_K1933100
location: https://synbiohub.org/public/igem/BBa_K1933100/annotation2516442/range2516442/1
component: BBa_K1933011

https://synbiohub.org/public/igem/BBa_K1933100/annotation2516442/range2516442/1
type: Range
persistent identity: range2516442
display id: range2516442
version: 1
direction: direction/reverse
top level: BBa_K1933100
start: 61
end: 1035
orientation: inline

CBDclos
type: ComponentDefinition
persistent identity: BBa K863111
display id: BBa_K863111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-17T11:00:00Z
modified: 2015-06-17T01:18:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans cellulose binding protein gene (cbp A) http://www.ncbi.nlm.nih.gov/nuccore/M73817
mutable notes: The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restriction-site) followed by 12 Bases (4 AS) of the cellulose binding protein gene in front of the CBD; then 12 bases (4 amino acids) of the cellulose binding protein gene behind the CBD; after that the sequence continues with a short GS-Linker and ends with the Freiburg-suffix (AgeI-restirction-site+TAA).
mutable provenance: Cloning-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: Cellulose Binding Protein
sequence annotation: Siltent mutation (A original)
sequence annotation: ATG
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: NgoMIV
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863111_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2185122
display id: annotation2185122
version: 1
top level: CBDclos
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185122/range2185122/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185122/range2185122/1
type: Range
persistent identity: range2185122
display id: range2185122
version: 1
direction: direction/forward
top level: CBDclos
start: 316
end: 318
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2185123
display id: annotation2185123
version: 1
top level: CBDclos
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185123/range2185123/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185123/range2185123/1
type: Range
persistent identity: range2185123
display id: range2185123
version: 1
direction: direction/forward
top level: CBDclos
start: 310
end: 315
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2185121
display id: annotation2185121
version: 1
top level: CBDclos
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185121/range2185121/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185121/range2185121/1
type: Range
persistent identity: range2185121
display id: range2185121
version: 1
direction: direction/forward
top level: CBDclos
start: 10
end: 21
orientation: inline

Siltent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2202131
display id: annotation2202131
version: 1
top level: CBDclos
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2202131/range2202131/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2202131/range2202131/1
type: Range
persistent identity: range2202131
display id: range2202131
version: 1
direction: direction/forward
top level: CBDclos
start: 93
end: 93
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2185119
display id: annotation2185119
version: 1
top level: CBDclos
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185119/range2185119/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185119/range2185119/1
type: Range
persistent identity: range2185119
display id: range2185119
version: 1
direction: direction/forward
top level: CBDclos
start: 1
end: 3
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2185126
display id: annotation2185126
version: 1
top level: CBDclos
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185126/range2185126/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185126/range2185126/1
type: Range
persistent identity: range2185126
display id: range2185126
version: 1
direction: direction/forward
top level: CBDclos
start: 298
end: 303
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2185124
display id: annotation2185124
version: 1
top level: CBDclos
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185124/range2185124/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185124/range2185124/1
type: Range
persistent identity: range2185124
display id: range2185124
version: 1
direction: direction/forward
top level: CBDclos
start: 22
end: 297
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2185120
display id: annotation2185120
version: 1
top level: CBDclos
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185120/range2185120/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185120/range2185120/1
type: Range
persistent identity: range2185120
display id: range2185120
version: 1
direction: direction/forward
top level: CBDclos
start: 4
end: 9
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2185127
display id: annotation2185127
version: 1
top level: CBDclos
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185127/range2185127/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185127/range2185127/1
type: Range
persistent identity: range2185127
display id: range2185127
version: 1
direction: direction/forward
top level: CBDclos
start: 304
end: 309
orientation: inline

BBa_K1321152
type: ComponentDefinition
persistent identity: BBa K1321152
display id: BBa_K1321152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metallothionein Fmt fused to CBDcex
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321012
component: BBa_K1321003
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321152_sequence/1

BBa_K1321012
type: Component
persistent identity: component2415037
display id: component2415037
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_K1321003
type: Component
persistent identity: component2415040
display id: component2415040
version: 1
top level: BBa_K1321152
access: public
definition: BBa_K1321003

BBa_B0105
type: Component
persistent identity: component2415039
display id: component2415039
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415039
display id: annotation2415039
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
type: Range
persistent identity: range2415039
display id: range2415039
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 199
end: 204
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415037
display id: annotation2415037
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
type: Range
persistent identity: range2415037
display id: range2415037
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 1
end: 198
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415040
display id: annotation2415040
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
type: Range
persistent identity: range2415040
display id: range2415040
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 205
end: 531
orientation: inline

BBa_K1321340
type: ComponentDefinition
persistent identity: BBa K1321340
display id: BBa_K1321340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double CBD (dCBD) with N-terminal linker
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: dCBD with N-terminal linker.
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: cbh1 CBD
sequence annotation: cbh2 CBD
sequence annotation: dCBD
sequence: https://synbiohub.org/public/igem/BBa_K1321340_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2417848
display id: annotation2417848
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1
type: Range
persistent identity: range2417848
display id: range2417848
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 1
end: 72
orientation: inline

cbh1 CBD
type: SequenceAnnotation
persistent identity: annotation2417851
display id: annotation2417851
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1
type: Range
persistent identity: range2417851
display id: range2417851
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 259
end: 366
orientation: inline

cbh2 CBD
type: SequenceAnnotation
persistent identity: annotation2417849
display id: annotation2417849
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1
type: Range
persistent identity: range2417849
display id: range2417849
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 73
end: 186
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417850
display id: annotation2417850
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1
type: Range
persistent identity: range2417850
display id: range2417850
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 187
end: 258
orientation: inline

dCBD
type: SequenceAnnotation
persistent identity: annotation2417847
display id: annotation2417847
version: 1
top level: BBa_K1321340
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1
type: Range
persistent identity: range2417847
display id: range2417847
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 1
end: 366
orientation: inline

BBa_K863110
type: ComponentDefinition
persistent identity: BBa K863110
display id: BBa_K863110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Clostridium cellulovorans cellulose binding protein
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:08Z
discontinued: false
dominant: false
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] in Freiburg-Standard.
mutable notes: Added Freiburg-pre- and suffix for in frame assembly and a two amino acids (GS)-Linker.
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CBP
sequence annotation: NgoMIV
sequence annotation: TAA
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: AgeI
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863110_sequence/1

CBP
type: SequenceAnnotation
persistent identity: annotation2188407
display id: annotation2188407
version: 1
top level: BBa_K863110
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188407/range2188407/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188407/range2188407/1
type: Range
persistent identity: range2188407
display id: range2188407
version: 1
direction: direction/forward
top level: BBa_K863110
start: 298
end: 303
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2188265
display id: annotation2188265
version: 1
top level: BBa_K863110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188265/range2188265/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188265/range2188265/1
type: Range
persistent identity: range2188265
display id: range2188265
version: 1
direction: direction/forward
top level: BBa_K863110
start: 4
end: 9
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188268
display id: annotation2188268
version: 1
top level: BBa_K863110
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188268/range2188268/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188268/range2188268/1
type: Range
persistent identity: range2188268
display id: range2188268
version: 1
direction: direction/forward
top level: BBa_K863110
start: 310
end: 312
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188266
display id: annotation2188266
version: 1
top level: BBa_K863110
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188266/range2188266/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188266/range2188266/1
type: Range
persistent identity: range2188266
display id: range2188266
version: 1
direction: direction/forward
top level: BBa_K863110
start: 22
end: 297
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188267
display id: annotation2188267
version: 1
top level: BBa_K863110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188267/range2188267/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188267/range2188267/1
type: Range
persistent identity: range2188267
display id: range2188267
version: 1
direction: direction/forward
top level: BBa_K863110
start: 304
end: 309
orientation: inline

CBP
type: SequenceAnnotation
persistent identity: annotation2188406
display id: annotation2188406
version: 1
top level: BBa_K863110
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188406/range2188406/1

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type: Range
persistent identity: range2188406
display id: range2188406
version: 1
direction: direction/forward
top level: BBa_K863110
start: 10
end: 21
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188264
display id: annotation2188264
version: 1
top level: BBa_K863110
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188264/range2188264/1

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type: Range
persistent identity: range2188264
display id: range2188264
version: 1
direction: direction/forward
top level: BBa_K863110
start: 1
end: 3
orientation: inline

BBa_K1321353
type: ComponentDefinition
persistent identity: BBa K1321353
display id: BBa_K1321353
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDclos driven by T7
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:32:03Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321338
component: BBa_K1319106
component: BBa_K1321343
sequence annotation: BBa_K1321343
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321353_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415403
display id: component2415403
version: 1
top level: BBa_K1321353
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1319106
type: Component
persistent identity: component2415405
display id: component2415405
version: 1
top level: BBa_K1321353
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321343
type: Component
persistent identity: component2415410
display id: component2415410
version: 1
top level: BBa_K1321353
access: public
definition: BBa_K1321343

BBa_K1321343
type: SequenceAnnotation
persistent identity: annotation2415410
display id: annotation2415410
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415410/range2415410/1
component: BBa_K1321343

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type: Range
persistent identity: range2415410
display id: range2415410
version: 1
direction: direction/reverse
top level: BBa_K1321353
start: 59
end: 540
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415403
display id: annotation2415403
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415403/range2415403/1
component: BBa_K1321338

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type: Range
persistent identity: range2415403
display id: range2415403
version: 1
direction: direction/reverse
top level: BBa_K1321353
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415405
display id: annotation2415405
version: 1
top level: BBa_K1321353
location: https://synbiohub.org/public/igem/BBa_K1321353/annotation2415405/range2415405/1
component: BBa_K1319106

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type: Range
persistent identity: range2415405
display id: range2415405
version: 1
direction: direction/reverse
top level: BBa_K1321353
start: 44
end: 58
orientation: inline

BBa_K1321345
type: ComponentDefinition
persistent identity: BBa K1321345
display id: BBa_K1321345
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:25:54Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1321009
component: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321009
sequence: https://synbiohub.org/public/igem/BBa_K1321345_sequence/1

BBa_K1321340
type: Component
persistent identity: component2415114
display id: component2415114
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321340

BBa_K1321009
type: Component
persistent identity: component2415110
display id: component2415110
version: 1
top level: BBa_K1321345
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321009/1

BBa_B0105
type: Component
persistent identity: component2415112
display id: component2415112
version: 1
top level: BBa_K1321345
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415114
display id: annotation2415114
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415114/range2415114/1
component: BBa_K1321340

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type: Range
persistent identity: range2415114
display id: range2415114
version: 1
direction: direction/reverse
top level: BBa_K1321345
start: 183
end: 548
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415112
display id: annotation2415112
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415112/range2415112/1
component: BBa_B0105

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type: Range
persistent identity: range2415112
display id: range2415112
version: 1
direction: direction/reverse
top level: BBa_K1321345
start: 177
end: 182
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415110
display id: annotation2415110
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
type: Range
persistent identity: range2415110
display id: range2415110
version: 1
direction: direction/reverse
top level: BBa_K1321345
start: 1
end: 176
orientation: inline

BBa_K1321101
type: ComponentDefinition
persistent identity: BBa K1321101
display id: BBa_K1321101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDclos with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD4
mutable notes: PhytoC+CBD4
mutable provenance: PhytoC+CBD4
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321338
component: BBa_B0105
component: BBa_K1321002
component: BBa_K1321005
component: BBa_K1319106
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321101_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415286
display id: component2415286
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_B0105
type: Component
persistent identity: component2415292
display id: component2415292
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2415293
display id: component2415293
version: 1
top level: BBa_K1321101
access: public
definition: BBa_K1321002

BBa_K1321005
type: Component
persistent identity: component2415290
display id: component2415290
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1319106
type: Component
persistent identity: component2415288
display id: component2415288
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415292
display id: annotation2415292
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415292/range2415292/1
component: BBa_B0105

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type: Range
persistent identity: range2415292
display id: range2415292
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 182
end: 187
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415290
display id: annotation2415290
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415290/range2415290/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415290/range2415290/1
type: Range
persistent identity: range2415290
display id: range2415290
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 59
end: 181
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415293
display id: annotation2415293
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415293/range2415293/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415293/range2415293/1
type: Range
persistent identity: range2415293
display id: range2415293
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 188
end: 487
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415286
display id: annotation2415286
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415286/range2415286/1
component: BBa_K1321338

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type: Range
persistent identity: range2415286
display id: range2415286
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415288
display id: annotation2415288
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415288/range2415288/1
component: BBa_K1319106

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type: Range
persistent identity: range2415288
display id: range2415288
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 44
end: 58
orientation: inline

BBa_K1321095
type: ComponentDefinition
persistent identity: BBa K1321095
display id: BBa_K1321095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to Phytochelatin (PC) EC20
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:34:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CBD7+PhytoC. TBC
mutable notes: CBD7+PhytoC. TBC
mutable provenance: CBD7+PhytoC. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321005
component: BBa_K1321003
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321095_sequence/1

BBa_B0105
type: Component
persistent identity: component2414729
display id: component2414729
version: 1
top level: BBa_K1321095
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2414731
display id: component2414731
version: 1
top level: BBa_K1321095
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1321003
type: Component
persistent identity: component2414727
display id: component2414727
version: 1
top level: BBa_K1321095
access: public
definition: BBa_K1321003

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414731
display id: annotation2414731
version: 1
top level: BBa_K1321095
location: https://synbiohub.org/public/igem/BBa_K1321095/annotation2414731/range2414731/1
component: BBa_K1321005

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type: Range
persistent identity: range2414731
display id: range2414731
version: 1
direction: direction/reverse
top level: BBa_K1321095
start: 350
end: 472
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414729
display id: annotation2414729
version: 1
top level: BBa_K1321095
location: https://synbiohub.org/public/igem/BBa_K1321095/annotation2414729/range2414729/1
component: BBa_B0105

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type: Range
persistent identity: range2414729
display id: range2414729
version: 1
direction: direction/reverse
top level: BBa_K1321095
start: 336
end: 341
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414727
display id: annotation2414727
version: 1
top level: BBa_K1321095
location: https://synbiohub.org/public/igem/BBa_K1321095/annotation2414727/range2414727/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321095/annotation2414727/range2414727/1
type: Range
persistent identity: range2414727
display id: range2414727
version: 1
direction: direction/reverse
top level: BBa_K1321095
start: 1
end: 327
orientation: inline

BBa_K1321002
type: ComponentDefinition
persistent identity: BBa K1321002
display id: BBa_K1321002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25
creator: Xenia Spencer-Milnes
created: 2014-10-09T11:00:00Z
modified: 2015-06-17T12:35:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a copy of BBa_K863111 (Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general.
mutable notes: This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
mutable provenance: This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1321002_sequence/1

BBa_K1321115
type: ComponentDefinition
persistent identity: BBa K1321115
display id: BBa_K1321115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to phytochelatin (PC) EC20 with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:20:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD7+PhytoC with LacI
mutable notes: CBD7+PhytoC with LacI
mutable provenance: CBD7+PhytoC with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J04500
component: BBa_K1321003
component: BBa_K1319106
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321115_sequence/1

BBa_J04500
type: Component
persistent identity: component2414978
display id: component2414978
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321003
type: Component
persistent identity: component2414981
display id: component2414981
version: 1
top level: BBa_K1321115
access: public
definition: BBa_K1321003

BBa_K1319106
type: Component
persistent identity: component2414980
display id: component2414980
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_B0105
type: Component
persistent identity: component2414983
display id: component2414983
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2414985
display id: component2414985
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414978
display id: annotation2414978
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414978/range2414978/1
component: BBa_J04500

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type: Range
persistent identity: range2414978
display id: range2414978
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 1
end: 220
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414985
display id: annotation2414985
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414985/range2414985/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414985/range2414985/1
type: Range
persistent identity: range2414985
display id: range2414985
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 569
end: 691
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414983
display id: annotation2414983
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414983/range2414983/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414983/range2414983/1
type: Range
persistent identity: range2414983
display id: range2414983
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 563
end: 568
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414981
display id: annotation2414981
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414981/range2414981/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414981/range2414981/1
type: Range
persistent identity: range2414981
display id: range2414981
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 236
end: 562
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414980
display id: annotation2414980
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414980/range2414980/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414980/range2414980/1
type: Range
persistent identity: range2414980
display id: range2414980
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 221
end: 235
orientation: inline

BBa_K1321093
type: ComponentDefinition
persistent identity: BBa K1321093
display id: BBa_K1321093
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 + Linker-CBDcipA-Linker
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:35:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1321014
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321014
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321093_sequence/1

BBa_K1321005
type: Component
persistent identity: component2423511
display id: component2423511
version: 1
top level: BBa_K1321093
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1321014
type: Component
persistent identity: component2423517
display id: component2423517
version: 1
top level: BBa_K1321093
access: public
definition: BBa_K1321014

BBa_B0105
type: Component
persistent identity: component2423513
display id: component2423513
version: 1
top level: BBa_K1321093
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2423513
display id: annotation2423513
version: 1
top level: BBa_K1321093
location: https://synbiohub.org/public/igem/BBa_K1321093/annotation2423513/range2423513/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321093/annotation2423513/range2423513/1
type: Range
persistent identity: range2423513
display id: range2423513
version: 1
direction: direction/reverse
top level: BBa_K1321093
start: 124
end: 129
orientation: inline

BBa_K1321014
type: SequenceAnnotation
persistent identity: annotation2423517
display id: annotation2423517
version: 1
top level: BBa_K1321093
location: https://synbiohub.org/public/igem/BBa_K1321093/annotation2423517/range2423517/1
component: BBa_K1321014

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type: Range
persistent identity: range2423517
display id: range2423517
version: 1
direction: direction/reverse
top level: BBa_K1321093
start: 130
end: 840
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2423511
display id: annotation2423511
version: 1
top level: BBa_K1321093
location: https://synbiohub.org/public/igem/BBa_K1321093/annotation2423511/range2423511/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321093/annotation2423511/range2423511/1
type: Range
persistent identity: range2423511
display id: range2423511
version: 1
direction: direction/reverse
top level: BBa_K1321093
start: 1
end: 123
orientation: inline

CBDcex
type: ComponentDefinition
persistent identity: BBa K863101
display id: BBa_K863101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-17T11:00:00Z
modified: 2015-06-17T01:18:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cellulose binding domain of the Cellulomonas fimi ATCC 484 exoglucanase gene
mutable notes: The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restrictionsite) followed by 12 Bases (4 AS) of the exoglucanase gene in front of the CBD. Then 9 bases of the exoglucanase gene behind the CBD. After that the sequence continues with a short GS-Linker.
mutable provenance: Cloning-Vector with a CBDcex-Barnase fusion-protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMIV
sequence annotation: GS-Linker
sequence annotation: Exoglucanase
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863101_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2184939
display id: annotation2184939
version: 1
top level: CBDcex
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184939/range2184939/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184939/range2184939/1
type: Range
persistent identity: range2184939
display id: range2184939
version: 1
direction: direction/forward
top level: CBDcex
start: 4
end: 9
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2184938
display id: annotation2184938
version: 1
top level: CBDcex
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184938/range2184938/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184938/range2184938/1
type: Range
persistent identity: range2184938
display id: range2184938
version: 1
direction: direction/forward
top level: CBDcex
start: 331
end: 336
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2184937
display id: annotation2184937
version: 1
top level: CBDcex
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184937/range2184937/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184937/range2184937/1
type: Range
persistent identity: range2184937
display id: range2184937
version: 1
direction: direction/forward
top level: CBDcex
start: 322
end: 330
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2184935
display id: annotation2184935
version: 1
top level: CBDcex
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184935/range2184935/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184935/range2184935/1
type: Range
persistent identity: range2184935
display id: range2184935
version: 1
direction: direction/forward
top level: CBDcex
start: 22
end: 321
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184934
display id: annotation2184934
version: 1
top level: CBDcex
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184934/range2184934/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184934/range2184934/1
type: Range
persistent identity: range2184934
display id: range2184934
version: 1
direction: direction/forward
top level: CBDcex
start: 343
end: 345
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2184940
display id: annotation2184940
version: 1
top level: CBDcex
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184940/range2184940/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184940/range2184940/1
type: Range
persistent identity: range2184940
display id: range2184940
version: 1
direction: direction/forward
top level: CBDcex
start: 337
end: 342
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2184936
display id: annotation2184936
version: 1
top level: CBDcex
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184936/range2184936/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184936/range2184936/1
type: Range
persistent identity: range2184936
display id: range2184936
version: 1
direction: direction/forward
top level: CBDcex
start: 10
end: 21
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184933
display id: annotation2184933
version: 1
top level: CBDcex
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184933/range2184933/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184933/range2184933/1
type: Range
persistent identity: range2184933
display id: range2184933
version: 1
direction: direction/forward
top level: CBDcex
start: 1
end: 3
orientation: inline

BBa_K1321014
type: ComponentDefinition
persistent identity: BBa K1321014
display id: BBa_K1321014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDCipA with N and C-terminal linker
creator: Xenia Spencer-Milnes
created: 2014-10-14T11:00:00Z
modified: 2015-06-17T12:14:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This CBD is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus (UniProt ID Q06851 link http://www.uniprot.org/uniprot/Q06851). It is in RFC25 format to allow for easy use in protein fusions. The CBD is part of the CBM3 family (link to cazy http://www.cazy.org/CBM3.html). This CBD has been used in many application: in fusions with cell adhesion peptides to enhance the properties of cellulose as a cell-growth matrix (Andrade et al 2010a, Andrade et al 2010b), fused to enzymes to remove contaminants from water (Kauffmann et al 2000) and fused to an antimicrobial peptide (Ramos, Domingues & Gama 2010). At present the cloning for these constructs is still in progress to correct an illegal EcoRI site which was identified in the parts with this CBD. This can be achieved with a silent mutation via site-directed mutagenesis and we aim to send these parts to the registry once this is complete.
mutable notes: The DNA sequence from the relevant portion of http://www.ncbi.nlm.nih.gov/nuccore/144777 was codon optimised for ''E.coli''. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.
mutable provenance: The designed construct was ordered from as a GeneArt?? String (Invitrogen??? Life Technologies).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBDcipA
sequence annotation: Endogenous Linker (C-term)
sequence annotation: Endogenous Linker (N-term)
sequence: https://synbiohub.org/public/igem/BBa_K1321014_sequence/1

CBDcipA
type: SequenceAnnotation
persistent identity: annotation2420186
display id: annotation2420186
version: 1
top level: BBa_K1321014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321014/annotation2420186/range2420186/1

https://synbiohub.org/public/igem/BBa_K1321014/annotation2420186/range2420186/1
type: Range
persistent identity: range2420186
display id: range2420186
version: 1
direction: direction/forward
top level: BBa_K1321014
start: 127
end: 603
orientation: inline

Endogenous Linker (C-term)
type: SequenceAnnotation
persistent identity: annotation2420187
display id: annotation2420187
version: 1
top level: BBa_K1321014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321014/annotation2420187/range2420187/1

https://synbiohub.org/public/igem/BBa_K1321014/annotation2420187/range2420187/1
type: Range
persistent identity: range2420187
display id: range2420187
version: 1
direction: direction/forward
top level: BBa_K1321014
start: 604
end: 711
orientation: inline

Endogenous Linker (N-term)
type: SequenceAnnotation
persistent identity: annotation2420141
display id: annotation2420141
version: 1
top level: BBa_K1321014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321014/annotation2420141/range2420141/1

https://synbiohub.org/public/igem/BBa_K1321014/annotation2420141/range2420141/1
type: Range
persistent identity: range2420141
display id: range2420141
version: 1
direction: direction/forward
top level: BBa_K1321014
start: 1
end: 126
orientation: inline

BBa_K1321112
type: ComponentDefinition
persistent identity: BBa K1321112
display id: BBa_K1321112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDcex with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD7 with LacI
mutable notes: PhytoC+CBD7 with LacI
mutable provenance: PhytoC+CBD7 with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J04500
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1319106
component: BBa_K1321005
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321112_sequence/1

BBa_J04500
type: Component
persistent identity: component2414911
display id: component2414911
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321003
type: Component
persistent identity: component2414918
display id: component2414918
version: 1
top level: BBa_K1321112
access: public
definition: BBa_K1321003

BBa_B0105
type: Component
persistent identity: component2414917
display id: component2414917
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1319106
type: Component
persistent identity: component2414913
display id: component2414913
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321005
type: Component
persistent identity: component2414915
display id: component2414915
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414911
display id: annotation2414911
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414911/range2414911/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414911/range2414911/1
type: Range
persistent identity: range2414911
display id: range2414911
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 1
end: 220
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414918
display id: annotation2414918
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414918/range2414918/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414918/range2414918/1
type: Range
persistent identity: range2414918
display id: range2414918
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 365
end: 691
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414917
display id: annotation2414917
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414917/range2414917/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414917/range2414917/1
type: Range
persistent identity: range2414917
display id: range2414917
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 359
end: 364
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414915
display id: annotation2414915
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414915/range2414915/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414915/range2414915/1
type: Range
persistent identity: range2414915
display id: range2414915
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 236
end: 358
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414913
display id: annotation2414913
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414913/range2414913/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414913/range2414913/1
type: Range
persistent identity: range2414913
display id: range2414913
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 221
end: 235
orientation: inline

BBa_K1321120
type: ComponentDefinition
persistent identity: BBa K1321120
display id: BBa_K1321120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA metallothionein fused to linker-dCBD
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_B0105
component: BBa_K1321011
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321011
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321120_sequence/1

BBa_K1321340
type: Component
persistent identity: component2414679
display id: component2414679
version: 1
top level: BBa_K1321120
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2414677
display id: component2414677
version: 1
top level: BBa_K1321120
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321011
type: Component
persistent identity: component2414675
display id: component2414675
version: 1
top level: BBa_K1321120
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414679
display id: annotation2414679
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
component: BBa_K1321340

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type: Range
persistent identity: range2414679
display id: range2414679
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 172
end: 537
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414675
display id: annotation2414675
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
component: BBa_K1321011

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type: Range
persistent identity: range2414675
display id: range2414675
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 1
end: 165
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414677
display id: annotation2414677
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
type: Range
persistent identity: range2414677
display id: range2414677
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 166
end: 171
orientation: inline

BBa_K1321094
type: ComponentDefinition
persistent identity: BBa K1321094
display id: BBa_K1321094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to Phytochelatin (PC) EC20
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC. TBC
mutable notes: CBD4+PhytoC. TBC
mutable provenance: CBD4+PhytoC. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321094_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414635
display id: component2414635
version: 1
top level: BBa_K1321094
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1321002
type: Component
persistent identity: component2414631
display id: component2414631
version: 1
top level: BBa_K1321094
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2414633
display id: component2414633
version: 1
top level: BBa_K1321094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414635
display id: annotation2414635
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
type: Range
persistent identity: range2414635
display id: range2414635
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 307
end: 429
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414631
display id: annotation2414631
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
type: Range
persistent identity: range2414631
display id: range2414631
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 1
end: 300
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414633
display id: annotation2414633
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
type: Range
persistent identity: range2414633
display id: range2414633
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 301
end: 306
orientation: inline

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
creator: Moritz M??ller
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
mutable notes: a switch to a const. Promoter
mutable provenance: different
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: His-Tag
sequence annotation: GS-Linker
sequence annotation: BBa_J23100
sequence annotation: BBa_K863121
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: Exoglucanase
sequence annotation: BBa_J61101
sequence annotation: BBa_E0040
sequence annotation: TAA
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: NgoMIV
sequence annotation: BBa_K863101
sequence annotation: Freiburg-Scar
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1118
end: 1135
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 392
end: 397
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1
end: 35
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1144
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1136
end: 1141
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 383
end: 391
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 71
end: 82
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 44
end: 55
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1117
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1142
end: 1144
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 83
end: 382
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 65
end: 70
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 397
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 398
end: 403
orientation: inline

BBa_K1478001
type: ComponentDefinition
persistent identity: BBa K1478001
display id: BBa_K1478001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-06-17T01:26:18Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for cellulose binding domain. When fused to a protein, protein attaches to cellulose
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Domain derived from cellulose binding protein from Chlostridium cellulovorans(WP_013291799).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478001_sequence/1

BBa_K1321105
type: ComponentDefinition
persistent identity: BBa K1321105
display id: BBa_K1321105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to Phytochelatin (PC) EC20 with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD7+PhytoC
mutable notes: CBD7+PhytoC
mutable provenance: CBD7+PhytoC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1319106
component: BBa_K1321338
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321338
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321105_sequence/1

BBa_K1321005
type: Component
persistent identity: component2415334
display id: component2415334
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_B0105
type: Component
persistent identity: component2415332
display id: component2415332
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1319106
type: Component
persistent identity: component2415329
display id: component2415329
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321338
type: Component
persistent identity: component2415327
display id: component2415327
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1321003
type: Component
persistent identity: component2415330
display id: component2415330
version: 1
top level: BBa_K1321105
access: public
definition: BBa_K1321003

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415330
display id: annotation2415330
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415330/range2415330/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415330/range2415330/1
type: Range
persistent identity: range2415330
display id: range2415330
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 59
end: 385
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415334
display id: annotation2415334
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415334/range2415334/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415334/range2415334/1
type: Range
persistent identity: range2415334
display id: range2415334
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 392
end: 514
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415327
display id: annotation2415327
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415327/range2415327/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415327/range2415327/1
type: Range
persistent identity: range2415327
display id: range2415327
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 1
end: 43
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415332
display id: annotation2415332
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415332/range2415332/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415332/range2415332/1
type: Range
persistent identity: range2415332
display id: range2415332
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 386
end: 391
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415329
display id: annotation2415329
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415329/range2415329/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321105/annotation2415329/range2415329/1
type: Range
persistent identity: range2415329
display id: range2415329
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 44
end: 58
orientation: inline

BBa_K1321357
type: ComponentDefinition
persistent identity: BBa K1321357
display id: BBa_K1321357
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K1321342
component: BBa_K1319106
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321342
sequence: https://synbiohub.org/public/igem/BBa_K1321357_sequence/1

BBa_J04500
type: Component
persistent identity: component2415499
display id: component2415499
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321342
type: Component
persistent identity: component2415507
display id: component2415507
version: 1
top level: BBa_K1321357
access: public
definition: BBa_K1321342

BBa_K1319106
type: Component
persistent identity: component2415501
display id: component2415501
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415499
display id: annotation2415499
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
type: Range
persistent identity: range2415499
display id: range2415499
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415501
display id: annotation2415501
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
type: Range
persistent identity: range2415501
display id: range2415501
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 221
end: 235
orientation: inline

BBa_K1321342
type: SequenceAnnotation
persistent identity: annotation2415507
display id: annotation2415507
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
component: BBa_K1321342

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
type: Range
persistent identity: range2415507
display id: range2415507
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 236
end: 1279
orientation: inline

BBa_K1321151
type: ComponentDefinition
persistent identity: BBa K1321151
display id: BBa_K1321151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metallothionein Fmt fused to CBDclos
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_B0105
component: BBa_K1321012
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321012
sequence: https://synbiohub.org/public/igem/BBa_K1321151_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415030
display id: component2415030
version: 1
top level: BBa_K1321151
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2415029
display id: component2415029
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321012
type: Component
persistent identity: component2415027
display id: component2415027
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415030
display id: annotation2415030
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
type: Range
persistent identity: range2415030
display id: range2415030
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 205
end: 504
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415029
display id: annotation2415029
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
type: Range
persistent identity: range2415029
display id: range2415029
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 199
end: 204
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415027
display id: annotation2415027
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
type: Range
persistent identity: range2415027
display id: range2415027
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 1
end: 198
orientation: inline

BBa_K1321348
type: ComponentDefinition
persistent identity: BBa K1321348
display id: BBa_K1321348
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_K1321340
component: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321348_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415136
display id: component2415136
version: 1
top level: BBa_K1321348
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321337/1

BBa_K1321340
type: Component
persistent identity: component2415140
display id: component2415140
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2415138
display id: component2415138
version: 1
top level: BBa_K1321348
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415140
display id: annotation2415140
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
type: Range
persistent identity: range2415140
display id: range2415140
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 718
end: 1083
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415138
display id: annotation2415138
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
type: Range
persistent identity: range2415138
display id: range2415138
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415136
display id: annotation2415136
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
type: Range
persistent identity: range2415136
display id: range2415136
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 1
end: 711
orientation: inline

BBa_K1979001
type: ComponentDefinition
persistent identity: BBa K1979001
display id: BBa_K1979001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD (cbh2)
creator: MA Xinyi
created: 2016-08-19T11:00:00Z
modified: 2016-08-24T10:46:39Z
discontinued: false
dominant: false
group u list: _2446_
m user id: 29797
owner id: 29797
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
mutable notes: Restriction sites Xho I and Bamh I are added.
mutable provenance: The sequence is retrieved from Genbank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBD cbh2
sequence: https://synbiohub.org/public/igem/BBa_K1979001_sequence/1

CBD cbh2
type: SequenceAnnotation
persistent identity: annotation2481557
display id: annotation2481557
version: 1
top level: BBa_K1979001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1979001/annotation2481557/range2481557/1

https://synbiohub.org/public/igem/BBa_K1979001/annotation2481557/range2481557/1
type: Range
persistent identity: range2481557
display id: range2481557
version: 1
direction: direction/forward
top level: BBa_K1979001
start: 1
end: 147
orientation: inline

BBa_K1321339
type: ComponentDefinition
persistent identity: BBa K1321339
display id: BBa_K1321339
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA+Linker, RFC 25 standard
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: CBDcenA in Freiburg format (RFC 25).
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBDCenA
sequence annotation: Linker: Pro-Thr box
sequence: https://synbiohub.org/public/igem/BBa_K1321339_sequence/1

CBDCenA
type: SequenceAnnotation
persistent identity: annotation2418065
display id: annotation2418065
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1

https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1
type: Range
persistent identity: range2418065
display id: range2418065
version: 1
direction: direction/forward
top level: BBa_K1321339
start: 1
end: 318
orientation: inline

Linker: Pro-Thr box
type: SequenceAnnotation
persistent identity: annotation2414614
display id: annotation2414614
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1

https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1
type: Range
persistent identity: range2414614
display id: range2414614
version: 1
direction: direction/forward
top level: BBa_K1321339
start: 319
end: 408
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: BBa_K863101
sequence annotation: BBa_K863121
sequence annotation: RBS
sequence annotation: GS-Linker
sequence annotation: Exoglucanase
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: Freiburg-Scar
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_K525998
sequence annotation: TAA
sequence annotation: BBa_K863102
sequence annotation: BBa_E0040
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1103
end: 1108
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 364
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1111
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1109
end: 1111
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 364
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1084
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1085
end: 1102
orientation: inline

BBa_K1321366
type: ComponentDefinition
persistent identity: BBa K1321366
display id: BBa_K1321366
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2016-01-26T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321338
component: BBa_K1321349
component: BBa_K1319106
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321349
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321366_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415724
display id: component2415724
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1321349
type: Component
persistent identity: component2415732
display id: component2415732
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321349

BBa_K1319106
type: Component
persistent identity: component2415726
display id: component2415726
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415724
display id: annotation2415724
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
type: Range
persistent identity: range2415724
display id: range2415724
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 1
end: 43
orientation: inline

BBa_K1321349
type: SequenceAnnotation
persistent identity: annotation2415732
display id: annotation2415732
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
component: BBa_K1321349

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
type: Range
persistent identity: range2415732
display id: range2415732
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 59
end: 1102
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415726
display id: annotation2415726
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
type: Range
persistent identity: range2415726
display id: range2415726
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 44
end: 58
orientation: inline

BBa_K1321100
type: ComponentDefinition
persistent identity: BBa K1321100
display id: BBa_K1321100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2 with T7. TBC
mutable notes: Phytochelatin EC(20) +CBD2 with T7. TBC
mutable provenance: Phytochelatin EC(20) +CBD2 with T7. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_B0105
component: BBa_K1321005
component: BBa_K1319106
component: BBa_K1321338
sequence annotation: BBa_K1321338
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321100_sequence/1

BBa_K1321340
type: Component
persistent identity: component2415255
display id: component2415255
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2415253
display id: component2415253
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2415251
display id: component2415251
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1319106
type: Component
persistent identity: component2415249
display id: component2415249
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321338
type: Component
persistent identity: component2415247
display id: component2415247
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415247
display id: annotation2415247
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
component: BBa_K1321338

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BBa_B0105
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BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415249
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BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415255
display id: annotation2415255
version: 1
top level: BBa_K1321100
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BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415251
display id: annotation2415251
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
component: BBa_K1321005

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start: 59
end: 181
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BBa_K1321349
type: ComponentDefinition
persistent identity: BBa K1321349
display id: BBa_K1321349
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:55Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321337
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321349_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415149
display id: component2415149
version: 1
top level: BBa_K1321349
access: public
definition: BBa_K1321003

BBa_B0105
type: Component
persistent identity: component2415151
display id: component2415151
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321337
type: Component
persistent identity: component2415153
display id: component2415153
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321337/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415151
display id: annotation2415151
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type: SequenceAnnotation
persistent identity: annotation2415149
display id: annotation2415149
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type: Range
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BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415153
display id: annotation2415153
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
component: BBa_K1321337

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type: Range
persistent identity: range2415153
display id: range2415153
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 334
end: 1044
orientation: inline

BBa_K1321104
type: ComponentDefinition
persistent identity: BBa K1321104
display id: BBa_K1321104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC
mutable notes: CBD4+PhytoC
mutable provenance: CBD4+PhytoC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1319106
component: BBa_K1321002
component: BBa_B0105
component: BBa_K1321338
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321104_sequence/1

BBa_K1321005
type: Component
persistent identity: component2420135
display id: component2420135
version: 1
top level: BBa_K1321104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1319106
type: Component
persistent identity: component2420130
display id: component2420130
version: 1
top level: BBa_K1321104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321002
type: Component
persistent identity: component2420131
display id: component2420131
version: 1
top level: BBa_K1321104
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
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display id: component2420133
version: 1
top level: BBa_K1321104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321338
type: Component
persistent identity: component2420128
display id: component2420128
version: 1
top level: BBa_K1321104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2420135
display id: annotation2420135
version: 1
top level: BBa_K1321104
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component: BBa_K1321005

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orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2420130
display id: annotation2420130
version: 1
top level: BBa_K1321104
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BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2420128
display id: annotation2420128
version: 1
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persistent identity: range2420128
display id: range2420128
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2420133
display id: annotation2420133
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420133/range2420133/1
component: BBa_B0105

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type: Range
persistent identity: range2420133
display id: range2420133
version: 1
direction: direction/reverse
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start: 359
end: 364
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2420131
display id: annotation2420131
version: 1
top level: BBa_K1321104
location: https://synbiohub.org/public/igem/BBa_K1321104/annotation2420131/range2420131/1
component: BBa_K1321002

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type: Range
persistent identity: range2420131
display id: range2420131
version: 1
direction: direction/reverse
top level: BBa_K1321104
start: 59
end: 358
orientation: inline

BBa_K1321351
type: ComponentDefinition
persistent identity: BBa K1321351
display id: BBa_K1321351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:45Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321339
component: BBa_K1321009
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321339
sequence: https://synbiohub.org/public/igem/BBa_K1321351_sequence/1

BBa_K1321339
type: Component
persistent identity: component2430307
display id: component2430307
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321339

BBa_K1321009
type: Component
persistent identity: component2430311
display id: component2430311
version: 1
top level: BBa_K1321351
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321009/1

BBa_B0105
type: Component
persistent identity: component2430309
display id: component2430309
version: 1
top level: BBa_K1321351
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2430309
display id: annotation2430309
version: 1
top level: BBa_K1321351
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component: BBa_B0105

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type: Range
persistent identity: range2430309
display id: range2430309
version: 1
direction: direction/reverse
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start: 409
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orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2430311
display id: annotation2430311
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430311/range2430311/1
component: BBa_K1321009

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type: Range
persistent identity: range2430311
display id: range2430311
version: 1
direction: direction/reverse
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orientation: inline

BBa_K1321339
type: SequenceAnnotation
persistent identity: annotation2430307
display id: annotation2430307
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430307/range2430307/1
component: BBa_K1321339

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type: Range
persistent identity: range2430307
display id: range2430307
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 1
end: 408
orientation: inline

BBa_K1321356
type: ComponentDefinition
persistent identity: BBa K1321356
display id: BBa_K1321356
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: awef
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K1319106
component: BBa_K1321341
sequence annotation: BBa_K1321341
sequence annotation: BBa_K1319106
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321356_sequence/1

BBa_J04500
type: Component
persistent identity: component2415481
display id: component2415481
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2415483
display id: component2415483
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321341
type: Component
persistent identity: component2415489
display id: component2415489
version: 1
top level: BBa_K1321356
access: public
definition: BBa_K1321341

BBa_K1321341
type: SequenceAnnotation
persistent identity: annotation2415489
display id: annotation2415489
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
component: BBa_K1321341

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type: Range
persistent identity: range2415489
display id: range2415489
version: 1
direction: direction/reverse
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start: 236
end: 1252
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2415483
display id: annotation2415483
version: 1
top level: BBa_K1321356
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persistent identity: range2415483
display id: range2415483
version: 1
direction: direction/reverse
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start: 221
end: 235
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415481
display id: annotation2415481
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
component: BBa_J04500

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type: Range
persistent identity: range2415481
display id: range2415481
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 1
end: 220
orientation: inline

BBa_K1933103
type: ComponentDefinition
persistent identity: BBa K1933103
display id: BBa_K1933103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDclos fused to BclA with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T11:05:25Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CBDclos fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321002
component: BBa_K1933004
component: BBa_K1933000
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933000
sequence: https://synbiohub.org/public/igem/BBa_K1933103_sequence/1

BBa_K1321002
type: Component
persistent identity: component2490481
display id: component2490481
version: 1
top level: BBa_K1933103
access: public
definition: BBa_K1321002

BBa_K1933004
type: Component
persistent identity: component2490476
display id: component2490476
version: 1
top level: BBa_K1933103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1933000
type: Component
persistent identity: component2490480
display id: component2490480
version: 1
top level: BBa_K1933103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933000/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2490481
display id: annotation2490481
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490481/range2490481/1
component: BBa_K1321002

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type: Range
persistent identity: range2490481
display id: range2490481
version: 1
direction: direction/reverse
top level: BBa_K1933103
start: 145
end: 444
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2490476
display id: annotation2490476
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490476/range2490476/1
component: BBa_K1933004

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type: Range
persistent identity: range2490476
display id: range2490476
version: 1
direction: direction/reverse
top level: BBa_K1933103
start: 1
end: 60
orientation: inline

BBa_K1933000
type: SequenceAnnotation
persistent identity: annotation2490480
display id: annotation2490480
version: 1
top level: BBa_K1933103
location: https://synbiohub.org/public/igem/BBa_K1933103/annotation2490480/range2490480/1
component: BBa_K1933000

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type: Range
persistent identity: range2490480
display id: range2490480
version: 1
direction: direction/reverse
top level: BBa_K1933103
start: 61
end: 144
orientation: inline

BBa_K1321114
type: ComponentDefinition
persistent identity: BBa K1321114
display id: BBa_K1321114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to phytochelatin (PC) EC20 with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC with LacI
mutable notes: CBD4+PhytoC with LacI
mutable provenance: CBD4+PhytoC with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_J04500
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1321002
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321114_sequence/1

BBa_K1319106
type: Component
persistent identity: component2414931
display id: component2414931
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_J04500
type: Component
persistent identity: component2414929
display id: component2414929
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321005
type: Component
persistent identity: component2414936
display id: component2414936
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_B0105
type: Component
persistent identity: component2414934
display id: component2414934
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2414932
display id: component2414932
version: 1
top level: BBa_K1321114
access: public
definition: BBa_K1321002

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414929
display id: annotation2414929
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414929/range2414929/1
component: BBa_J04500

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type: Range
persistent identity: range2414929
display id: range2414929
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start: 1
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orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414932
display id: annotation2414932
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414932/range2414932/1
component: BBa_K1321002

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type: Range
persistent identity: range2414932
display id: range2414932
version: 1
direction: direction/reverse
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start: 236
end: 535
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414936
display id: annotation2414936
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414936/range2414936/1
component: BBa_K1321005

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type: Range
persistent identity: range2414936
display id: range2414936
version: 1
direction: direction/reverse
top level: BBa_K1321114
start: 542
end: 664
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414931
display id: annotation2414931
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414931/range2414931/1
component: BBa_K1319106

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type: Range
persistent identity: range2414931
display id: range2414931
version: 1
direction: direction/reverse
top level: BBa_K1321114
start: 221
end: 235
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414934
display id: annotation2414934
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414934/range2414934/1
component: BBa_B0105

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type: Range
persistent identity: range2414934
display id: range2414934
version: 1
direction: direction/reverse
top level: BBa_K1321114
start: 536
end: 541
orientation: inline

BBa_K1933101
type: ComponentDefinition
persistent identity: BBa K1933101
display id: BBa_K1933101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDclos fused to INPNC with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-18T11:01:45Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CBDclos fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBDclos) fused to surface expression anchoring domain(INPNC), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: Promoter and RBS: iGEM16_Kyoto The INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. CBDclos derives from Clostridium cellulovorans, and is part of a multi-unit cellulosomes. We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321002
component: BBa_K1933011
component: BBa_K1933004
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933011
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1933101_sequence/1

BBa_K1321002
type: Component
persistent identity: component2515619
display id: component2515619
version: 1
top level: BBa_K1933101
access: public
definition: BBa_K1321002

BBa_K1933011
type: Component
persistent identity: component2515618
display id: component2515618
version: 1
top level: BBa_K1933101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933011/1

BBa_K1933004
type: Component
persistent identity: component2515614
display id: component2515614
version: 1
top level: BBa_K1933101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2515614
display id: annotation2515614
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515614/range2515614/1
component: BBa_K1933004

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type: Range
persistent identity: range2515614
display id: range2515614
version: 1
direction: direction/reverse
top level: BBa_K1933101
start: 1
end: 60
orientation: inline

BBa_K1933011
type: SequenceAnnotation
persistent identity: annotation2515618
display id: annotation2515618
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515618/range2515618/1
component: BBa_K1933011

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type: Range
persistent identity: range2515618
display id: range2515618
version: 1
direction: direction/reverse
top level: BBa_K1933101
start: 61
end: 1035
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2515619
display id: annotation2515619
version: 1
top level: BBa_K1933101
location: https://synbiohub.org/public/igem/BBa_K1933101/annotation2515619/range2515619/1
component: BBa_K1321002

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type: Range
persistent identity: range2515619
display id: range2515619
version: 1
direction: direction/reverse
top level: BBa_K1933101
start: 1036
end: 1335
orientation: inline

BBa_K1321344
type: ComponentDefinition
persistent identity: BBa K1321344
display id: BBa_K1321344
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:09Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321009
sequence annotation: BBa_K1321009
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321344_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415107
display id: component2415107
version: 1
top level: BBa_K1321344
access: public
definition: BBa_K1321003

BBa_B0105
type: Component
persistent identity: component2415106
display id: component2415106
version: 1
top level: BBa_K1321344
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: Component
persistent identity: component2415104
display id: component2415104
version: 1
top level: BBa_K1321344
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321009/1

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415104
display id: annotation2415104
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
component: BBa_K1321009

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type: Range
persistent identity: range2415104
display id: range2415104
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 1
end: 176
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415106
display id: annotation2415106
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
component: BBa_B0105

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type: Range
persistent identity: range2415106
display id: range2415106
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 177
end: 182
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415107
display id: annotation2415107
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
component: BBa_K1321003

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type: Range
persistent identity: range2415107
display id: range2415107
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 183
end: 509
orientation: inline

BBa_K1321090
type: ComponentDefinition
persistent identity: BBa K1321090
display id: BBa_K1321090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 + Linker-dCBD
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2. TBC
mutable notes: Codon optimised for E.coli. TBC
mutable provenance: CBD2 is EnLinker-CBH2-EnLinker-CBH1 from T.reesei. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1321005
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321090_sequence/1

BBa_K1321340
type: Component
persistent identity: component2414673
display id: component2414673
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321340

BBa_K1321005
type: Component
persistent identity: component2414669
display id: component2414669
version: 1
top level: BBa_K1321090
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_B0105
type: Component
persistent identity: component2414671
display id: component2414671
version: 1
top level: BBa_K1321090
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414671
display id: annotation2414671
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
component: BBa_B0105

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type: Range
persistent identity: range2414671
display id: range2414671
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 124
end: 129
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414673
display id: annotation2414673
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
component: BBa_K1321340

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type: Range
persistent identity: range2414673
display id: range2414673
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 130
end: 495
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414669
display id: annotation2414669
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
component: BBa_K1321005

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type: Range
persistent identity: range2414669
display id: range2414669
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 1
end: 123
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863102
display id: BBa_K863102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-18T11:00:00Z
modified: 2015-06-17T01:19:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard with T7, RBS, GS-Linker (Freiburg-Standard)
mutable notes: You can find additional info to the Cellulose binding domain here: [http://partsregistry.org/Part:BBa_K863101:Design CBDcex_Freiburg]
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K525998
sequence annotation: Exoglucanase
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: GS-Linker
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: BBa_K863101
sequence annotation: ATG
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K863102_sequence/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2187504
display id: annotation2187504
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1
type: Range
persistent identity: range2187504
display id: range2187504
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187508
display id: annotation2187508
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1
type: Range
persistent identity: range2187508
display id: range2187508
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187514
display id: annotation2187514
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1
type: Range
persistent identity: range2187514
display id: range2187514
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 373
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2187505
display id: annotation2187505
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1
type: Range
persistent identity: range2187505
display id: range2187505
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2187510
display id: annotation2187510
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1
type: Range
persistent identity: range2187510
display id: range2187510
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2187512
display id: annotation2187512
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1
type: Range
persistent identity: range2187512
display id: range2187512
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187511
display id: annotation2187511
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1
type: Range
persistent identity: range2187511
display id: range2187511
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187513
display id: annotation2187513
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1
type: Range
persistent identity: range2187513
display id: range2187513
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187506
display id: annotation2187506
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1
type: Range
persistent identity: range2187506
display id: range2187506
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2187509
display id: annotation2187509
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1
type: Range
persistent identity: range2187509
display id: range2187509
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 373
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187507
display id: annotation2187507
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1
type: Range
persistent identity: range2187507
display id: range2187507
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2187503
display id: annotation2187503
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1
type: Range
persistent identity: range2187503
display id: range2187503
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

BBa_K1321343
type: ComponentDefinition
persistent identity: BBa K1321343
display id: BBa_K1321343
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:16Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: adsdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321002
component: BBa_K1321009
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321009
sequence: https://synbiohub.org/public/igem/BBa_K1321343_sequence/1

BBa_B0105
type: Component
persistent identity: component2415100
display id: component2415100
version: 1
top level: BBa_K1321343
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2415101
display id: component2415101
version: 1
top level: BBa_K1321343
access: public
definition: BBa_K1321002

BBa_K1321009
type: Component
persistent identity: component2415098
display id: component2415098
version: 1
top level: BBa_K1321343
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321009/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415101
display id: annotation2415101
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
type: Range
persistent identity: range2415101
display id: range2415101
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 183
end: 482
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415100
display id: annotation2415100
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
type: Range
persistent identity: range2415100
display id: range2415100
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 177
end: 182
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415098
display id: annotation2415098
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
component: BBa_K1321009

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type: Range
persistent identity: range2415098
display id: range2415098
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 1
end: 176
orientation: inline

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
creator: Moritz M??ller
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS (<partinfo>J61101</partinfo>) and a fused-GFP protein with His-tag.
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
mutable provenance: Cellulose binding domain form a cloning-vector. <partinfo>J61101</partinfo> and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Cellulose Binding Protein
sequence annotation: ATG
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863121
sequence annotation: BBa_K863111
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: BBa_J23100
sequence annotation: GS-Linker
sequence annotation: His-Tag
sequence annotation: BBa_J61101
sequence annotation: TAA
sequence annotation: BBa_E0040
sequence annotation: Cellulose binding domain (CBDclos)
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

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type: Range
persistent identity: range2194134
display id: range2194134
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 71
end: 82
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1
type: Range
persistent identity: range2194132
display id: range2194132
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 62
end: 64
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 371
end: 376
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 377
end: 1117
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1
type: Range
persistent identity: range2194131
display id: range2194131
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 62
end: 370
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1
type: Range
persistent identity: range2194142
display id: range2194142
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1109
end: 1114
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 65
end: 70
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 359
end: 364
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 1
end: 35
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1
type: Range
persistent identity: range2194137
display id: range2194137
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 365
end: 370
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1
type: Range
persistent identity: range2194141
display id: range2194141
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1091
end: 1108
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 44
end: 55
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1115
end: 1117
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1
type: Range
persistent identity: range2194140
display id: range2194140
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 377
end: 1090
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 83
end: 358
orientation: inline

BBa_K1499004
type: ComponentDefinition
persistent identity: BBa K1499004
display id: BBa_K1499004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domains with streptavidin domain generator
creator: Raman Nelakanti
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:53:51Z
discontinued: false
dominant: false
group u list: _1879_
m user id: 4206
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: Different sequences for cellulose-binding domains to prevent homologous recombination. Removed cut sites for RFC-10 compatibility.
mutable provenance: organism 1 organism 2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Cellulose-binding(clostridium cellulovorans)
sequence annotation: Streptavidin
sequence: https://synbiohub.org/public/igem/BBa_K1499004_sequence/1

Cellulose-binding(clostridium cellulovorans)
type: SequenceAnnotation
persistent identity: annotation2429345
display id: annotation2429345
version: 1
top level: BBa_K1499004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1499004/annotation2429345/range2429345/1

https://synbiohub.org/public/igem/BBa_K1499004/annotation2429345/range2429345/1
type: Range
persistent identity: range2429345
display id: range2429345
version: 1
direction: direction/forward
top level: BBa_K1499004
start: 55
end: 352
orientation: inline

Streptavidin
type: SequenceAnnotation
persistent identity: annotation2429346
display id: annotation2429346
version: 1
top level: BBa_K1499004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1499004/annotation2429346/range2429346/1

https://synbiohub.org/public/igem/BBa_K1499004/annotation2429346/range2429346/1
type: Range
persistent identity: range2429346
display id: range2429346
version: 1
direction: direction/forward
top level: BBa_K1499004
start: 373
end: 750
orientation: inline

BBa_K1321342
type: ComponentDefinition
persistent identity: BBa K1321342
display id: BBa_K1321342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:42Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321337
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321342_sequence/1

BBa_B0105
type: Component
persistent identity: component2415361
display id: component2415361
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321337
type: Component
persistent identity: component2415359
display id: component2415359
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321337/1

BBa_K1321003
type: Component
persistent identity: component2415362
display id: component2415362
version: 1
top level: BBa_K1321342
access: public
definition: BBa_K1321003

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415362
display id: annotation2415362
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
type: Range
persistent identity: range2415362
display id: range2415362
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 718
end: 1044
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415361
display id: annotation2415361
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
type: Range
persistent identity: range2415361
display id: range2415361
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415359
display id: annotation2415359
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
type: Range
persistent identity: range2415359
display id: range2415359
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 1
end: 711
orientation: inline

BBa_K1321092
type: ComponentDefinition
persistent identity: BBa K1321092
display id: BBa_K1321092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDcex
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD7. TBC
mutable notes: PhytoC+CBD7. TBC
mutable provenance: PhytoC+CBD7. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321092_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414618
display id: component2414618
version: 1
top level: BBa_K1321092
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_B0105
type: Component
persistent identity: component2414620
display id: component2414620
version: 1
top level: BBa_K1321092
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2414621
display id: component2414621
version: 1
top level: BBa_K1321092
access: public
definition: BBa_K1321003

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414620
display id: annotation2414620
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
type: Range
persistent identity: range2414620
display id: range2414620
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 124
end: 129
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414618
display id: annotation2414618
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
type: Range
persistent identity: range2414618
display id: range2414618
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 1
end: 123
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414621
display id: annotation2414621
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
type: Range
persistent identity: range2414621
display id: range2414621
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 130
end: 456
orientation: inline

BBa_K1321341
type: ComponentDefinition
persistent identity: BBa K1321341
display id: BBa_K1321341
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:02Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdfads
mutable notes: asdfd
mutable provenance: asdfad
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_B0105
component: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321341_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415088
display id: component2415088
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321337/1

BBa_B0105
type: Component
persistent identity: component2415090
display id: component2415090
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2415091
display id: component2415091
version: 1
top level: BBa_K1321341
access: public
definition: BBa_K1321002

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415090
display id: annotation2415090
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
type: Range
persistent identity: range2415090
display id: range2415090
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 712
end: 717
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415091
display id: annotation2415091
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
type: Range
persistent identity: range2415091
display id: range2415091
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 718
end: 1017
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415088
display id: annotation2415088
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
type: Range
persistent identity: range2415088
display id: range2415088
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 1
end: 711
orientation: inline

BBa_K1321097
type: ComponentDefinition
persistent identity: BBa K1321097
display id: BBa_K1321097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA-linker fused to Phytochelatin (PC) EC20
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:34:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CBD15+PhytoC. TBC
mutable notes: CBD15+PhytoC. TBC
mutable provenance: CBD15+PhytoC. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1321340
component: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321340
sequence: https://synbiohub.org/public/igem/BBa_K1321097_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414744
display id: component2414744
version: 1
top level: BBa_K1321097
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1321340
type: Component
persistent identity: component2414740
display id: component2414740
version: 1
top level: BBa_K1321097
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2414742
display id: component2414742
version: 1
top level: BBa_K1321097
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414744
display id: annotation2414744
version: 1
top level: BBa_K1321097
location: https://synbiohub.org/public/igem/BBa_K1321097/annotation2414744/range2414744/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321097/annotation2414744/range2414744/1
type: Range
persistent identity: range2414744
display id: range2414744
version: 1
direction: direction/reverse
top level: BBa_K1321097
start: 389
end: 511
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414742
display id: annotation2414742
version: 1
top level: BBa_K1321097
location: https://synbiohub.org/public/igem/BBa_K1321097/annotation2414742/range2414742/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321097/annotation2414742/range2414742/1
type: Range
persistent identity: range2414742
display id: range2414742
version: 1
direction: direction/reverse
top level: BBa_K1321097
start: 375
end: 380
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414740
display id: annotation2414740
version: 1
top level: BBa_K1321097
location: https://synbiohub.org/public/igem/BBa_K1321097/annotation2414740/range2414740/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321097/annotation2414740/range2414740/1
type: Range
persistent identity: range2414740
display id: range2414740
version: 1
direction: direction/reverse
top level: BBa_K1321097
start: 1
end: 366
orientation: inline

CBDclos(T7
type: ComponentDefinition
persistent identity: BBa K863112
display id: BBa_K863112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard
mutable notes: Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Cellulose Binding Protein
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: GS-Linker
sequence annotation: silent mutation (A original)
sequence annotation: TAA
sequence annotation: BBa_K863111
sequence annotation: AgeI
sequence annotation: T7 promoter
sequence annotation: Cellulose binding domain (CBDclos)
sequence: https://synbiohub.org/public/igem/BBa_K863112_sequence/1

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188288
display id: annotation2188288
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1
type: Range
persistent identity: range2188288
display id: range2188288
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 40
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188290
display id: annotation2188290
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1
type: Range
persistent identity: range2188290
display id: range2188290
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 317
end: 322
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188287
display id: annotation2188287
version: 1
top level: CBDclos(T7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1
type: Range
persistent identity: range2188287
display id: range2188287
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 38
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188284
display id: annotation2188284
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1
type: Range
persistent identity: range2188284
display id: range2188284
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 21
end: 32
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188285
display id: annotation2188285
version: 1
top level: CBDclos(T7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1
type: Range
persistent identity: range2188285
display id: range2188285
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188283
display id: annotation2188283
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1
type: Range
persistent identity: range2188283
display id: range2188283
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 1
end: 32
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188291
display id: annotation2188291
version: 1
top level: CBDclos(T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1
type: Range
persistent identity: range2188291
display id: range2188291
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 323
end: 328
orientation: inline

silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200498
display id: annotation2200498
version: 1
top level: CBDclos(T7
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1
type: Range
persistent identity: range2200498
display id: range2200498
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 112
end: 112
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188293
display id: annotation2188293
version: 1
top level: CBDclos(T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1
type: Range
persistent identity: range2188293
display id: range2188293
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 335
end: 337
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188286
display id: annotation2188286
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1
type: Range
persistent identity: range2188286
display id: range2188286
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 337
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188292
display id: annotation2188292
version: 1
top level: CBDclos(T7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1
type: Range
persistent identity: range2188292
display id: range2188292
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 329
end: 334
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188282
display id: annotation2188282
version: 1
top level: CBDclos(T7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1
type: Range
persistent identity: range2188282
display id: range2188282
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 1
end: 20
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188289
display id: annotation2188289
version: 1
top level: CBDclos(T7
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1
type: Range
persistent identity: range2188289
display id: range2188289
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 41
end: 316
orientation: inline

BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0040
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_K525998
sequence annotation: GS-Linker
sequence annotation: T7 promoter
sequence annotation: BBa_K863111
sequence annotation: ATG
sequence annotation: His-Tag
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: RBS
sequence annotation: AgeI
sequence annotation: BBa_K863112
sequence annotation: Silent mutation (A original)
sequence annotation: BBa_K863121
sequence annotation: Freiburg-Scar
sequence annotation: B0034
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
direction: direction/forward
top level: BBa_K863113
start: 335
end: 1048
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 40
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 32
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
direction: direction/forward
top level: BBa_K863113
start: 323
end: 328
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 20
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 328
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 38
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1049
end: 1066
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
direction: direction/forward
top level: BBa_K863113
start: 41
end: 316
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
direction: direction/forward
top level: BBa_K863113
start: 24
end: 27
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1068
end: 1072
orientation: inline

BBa_K863112
type: SequenceAnnotation
persistent identity: annotation2188386
display id: annotation2188386
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 1
end: 328
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
direction: direction/forward
top level: BBa_K863113
start: 112
end: 112
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 335
end: 1075
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
direction: direction/forward
top level: BBa_K863113
start: 329
end: 334
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

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type: Range
persistent identity: range2188389
display id: range2188389
version: 1
direction: direction/forward
top level: BBa_K863113
start: 21
end: 32
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

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type: Range
persistent identity: range2188402
display id: range2188402
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1073
end: 1075
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1
type: Range
persistent identity: range2188395
display id: range2188395
version: 1
direction: direction/forward
top level: BBa_K863113
start: 317
end: 322
orientation: inline

BBa_K1321121
type: ComponentDefinition
persistent identity: BBa K1321121
display id: BBa_K1321121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt metallothionein fused to CBDclos
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1321011
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321011
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321121_sequence/1

BBa_K1321011
type: Component
persistent identity: component2414703
display id: component2414703
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1321002
type: Component
persistent identity: component2414706
display id: component2414706
version: 1
top level: BBa_K1321121
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2414705
display id: component2414705
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414706
display id: annotation2414706
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
type: Range
persistent identity: range2414706
display id: range2414706
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 172
end: 471
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414703
display id: annotation2414703
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
type: Range
persistent identity: range2414703
display id: range2414703
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 1
end: 165
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414705
display id: annotation2414705
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
type: Range
persistent identity: range2414705
display id: range2414705
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 166
end: 171
orientation: inline

BBa_K1321111
type: ComponentDefinition
persistent identity: BBa K1321111
display id: BBa_K1321111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD4 with LacI
mutable notes: PhytoC+CBD4 with LacI
mutable provenance: PhytoC+CBD4 with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1319106
component: BBa_K1321002
component: BBa_B0105
component: BBa_J04500
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321005
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321111_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414889
display id: component2414889
version: 1
top level: BBa_K1321111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_K1319106
type: Component
persistent identity: component2414887
display id: component2414887
version: 1
top level: BBa_K1321111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321002
type: Component
persistent identity: component2414892
display id: component2414892
version: 1
top level: BBa_K1321111
access: public
definition: BBa_K1321002

BBa_B0105
type: Component
persistent identity: component2414891
display id: component2414891
version: 1
top level: BBa_K1321111
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J04500
type: Component
persistent identity: component2414885
display id: component2414885
version: 1
top level: BBa_K1321111
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414891
display id: annotation2414891
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414891/range2414891/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414891/range2414891/1
type: Range
persistent identity: range2414891
display id: range2414891
version: 1
direction: direction/reverse
top level: BBa_K1321111
start: 359
end: 364
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414887
display id: annotation2414887
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414887/range2414887/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414887/range2414887/1
type: Range
persistent identity: range2414887
display id: range2414887
version: 1
direction: direction/reverse
top level: BBa_K1321111
start: 221
end: 235
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414892
display id: annotation2414892
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414892/range2414892/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414892/range2414892/1
type: Range
persistent identity: range2414892
display id: range2414892
version: 1
direction: direction/reverse
top level: BBa_K1321111
start: 365
end: 664
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414889
display id: annotation2414889
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414889/range2414889/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414889/range2414889/1
type: Range
persistent identity: range2414889
display id: range2414889
version: 1
direction: direction/reverse
top level: BBa_K1321111
start: 236
end: 358
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414885
display id: annotation2414885
version: 1
top level: BBa_K1321111
location: https://synbiohub.org/public/igem/BBa_K1321111/annotation2414885/range2414885/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321111/annotation2414885/range2414885/1
type: Range
persistent identity: range2414885
display id: range2414885
version: 1
direction: direction/reverse
top level: BBa_K1321111
start: 1
end: 220
orientation: inline

BBa_K1933102
type: ComponentDefinition
persistent identity: BBa K1933102
display id: BBa_K1933102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive expression of CBDcex fused to BclA with 6xHis tag
creator: Tomoki Uchino
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T12:04:53Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2400_
m user id: 23701
owner id: 29998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CBDcex fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a cellulose binding protein(CBD) fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Westernblotting. For more information, please visit our Wiki page.
mutable notes: The stop codon for this part is not in the coding region, but in the suffix region.
mutable provenance: Promoter and RBS:BBa_K1933004 The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We used parts BBa_K1933000 from iGEMKyoto 2016 for construction. We outsourced chemical synthesis of this part for construction. CBDcex derives from Cellulomonas fimi, and is part of an exoglucanase secreted by this organism. We used parts BBa_K1321342 from iGEM Imperial 2014 for construction.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1933000
component: BBa_K1933004
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1933004
sequence annotation: BBa_K1933000
sequence: https://synbiohub.org/public/igem/BBa_K1933102_sequence/1

BBa_K1933000
type: Component
persistent identity: component2491091
display id: component2491091
version: 1
top level: BBa_K1933102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933000/1

BBa_K1933004
type: Component
persistent identity: component2491087
display id: component2491087
version: 1
top level: BBa_K1933102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1933004/1

BBa_K1321003
type: Component
persistent identity: component2491092
display id: component2491092
version: 1
top level: BBa_K1933102
access: public
definition: BBa_K1321003

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2491092
display id: annotation2491092
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491092/range2491092/1
component: BBa_K1321003

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type: Range
persistent identity: range2491092
display id: range2491092
version: 1
direction: direction/reverse
top level: BBa_K1933102
start: 145
end: 471
orientation: inline

BBa_K1933004
type: SequenceAnnotation
persistent identity: annotation2491087
display id: annotation2491087
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491087/range2491087/1
component: BBa_K1933004

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491087/range2491087/1
type: Range
persistent identity: range2491087
display id: range2491087
version: 1
direction: direction/reverse
top level: BBa_K1933102
start: 1
end: 60
orientation: inline

BBa_K1933000
type: SequenceAnnotation
persistent identity: annotation2491091
display id: annotation2491091
version: 1
top level: BBa_K1933102
location: https://synbiohub.org/public/igem/BBa_K1933102/annotation2491091/range2491091/1
component: BBa_K1933000

https://synbiohub.org/public/igem/BBa_K1933102/annotation2491091/range2491091/1
type: Range
persistent identity: range2491091
display id: range2491091
version: 1
direction: direction/reverse
top level: BBa_K1933102
start: 61
end: 144
orientation: inline

BBa_K1321096
type: ComponentDefinition
persistent identity: BBa K1321096
display id: BBa_K1321096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker-CBDcipA-Linker + Phytochelatin (PC) EC20
creator: Xenia Spencer-Milnes
created: 2014-10-16T11:00:00Z
modified: 2015-06-17T12:35:09Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence. At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA. This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
mutable notes: The linker sequences mean fusions should be fine either N or N-terminally to the CBDcipA
mutable provenance: Please see part pages for the composite parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1321014
sequence annotation: BBa_K1321014
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321096_sequence/1

BBa_K1321005
type: Component
persistent identity: component2423607
display id: component2423607
version: 1
top level: BBa_K1321096
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321005/1

BBa_B0105
type: Component
persistent identity: component2423605
display id: component2423605
version: 1
top level: BBa_K1321096
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321014
type: Component
persistent identity: component2423603
display id: component2423603
version: 1
top level: BBa_K1321096
access: public
definition: BBa_K1321014

BBa_K1321014
type: SequenceAnnotation
persistent identity: annotation2423603
display id: annotation2423603
version: 1
top level: BBa_K1321096
location: https://synbiohub.org/public/igem/BBa_K1321096/annotation2423603/range2423603/1
component: BBa_K1321014

https://synbiohub.org/public/igem/BBa_K1321096/annotation2423603/range2423603/1
type: Range
persistent identity: range2423603
display id: range2423603
version: 1
direction: direction/reverse
top level: BBa_K1321096
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2423605
display id: annotation2423605
version: 1
top level: BBa_K1321096
location: https://synbiohub.org/public/igem/BBa_K1321096/annotation2423605/range2423605/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321096/annotation2423605/range2423605/1
type: Range
persistent identity: range2423605
display id: range2423605
version: 1
direction: direction/reverse
top level: BBa_K1321096
start: 712
end: 717
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2423607
display id: annotation2423607
version: 1
top level: BBa_K1321096
location: https://synbiohub.org/public/igem/BBa_K1321096/annotation2423607/range2423607/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321096/annotation2423607/range2423607/1
type: Range
persistent identity: range2423607
display id: range2423607
version: 1
direction: direction/reverse
top level: BBa_K1321096
start: 718
end: 840
orientation: inline

BBa_K1321352
type: ComponentDefinition
persistent identity: BBa K1321352
display id: BBa_K1321352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDcex driven by T7
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:54Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321344
component: BBa_K1321338
component: BBa_K1319106
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321344
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321352_sequence/1

BBa_K1321344
type: Component
persistent identity: component2415435
display id: component2415435
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321344

BBa_K1321338
type: Component
persistent identity: component2415428
display id: component2415428
version: 1
top level: BBa_K1321352
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1319106
type: Component
persistent identity: component2415430
display id: component2415430
version: 1
top level: BBa_K1321352
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415428
display id: annotation2415428
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
type: Range
persistent identity: range2415428
display id: range2415428
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 1
end: 43
orientation: inline

BBa_K1321344
type: SequenceAnnotation
persistent identity: annotation2415435
display id: annotation2415435
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
component: BBa_K1321344

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
type: Range
persistent identity: range2415435
display id: range2415435
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 59
end: 567
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415430
display id: annotation2415430
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
type: Range
persistent identity: range2415430
display id: range2415430
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 44
end: 58
orientation: inline

BBa_K1321003
type: ComponentDefinition
persistent identity: BBa K1321003
display id: BBa_K1321003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Re-entry of BBa_K863101, Cellulose Binding Domain CBDcex in rfc25
creator: Xenia Spencer-Milnes
created: 2014-10-09T11:00:00Z
modified: 2015-06-17T12:35:33Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a copy of BBa_K863101 (Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general.
mutable notes: This is a direct copy of BBa_K863101 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
mutable provenance: This is a direct copy of BBa_K863101 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1321003_sequence/1

BBa_K1321154
type: ComponentDefinition
persistent identity: BBa K1321154
display id: BBa_K1321154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to metallothionein Fmt
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321012
component: BBa_B0105
component: BBa_K1321002
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321012
sequence annotation: BBa_B0105
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BBa_K1321002
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definition: BBa_K1321002

BBa_K1321002
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BBa_K1321110
type: ComponentDefinition
persistent identity: BBa K1321110
display id: BBa_K1321110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2 with LacI
mutable notes: Phytochelatin EC(20) +CBD2 with LacI
mutable provenance: Phytochelatin EC(20) +CBD2 with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J04500
component: BBa_B0105
component: BBa_K1319106
component: BBa_K1321340
component: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321110_sequence/1

BBa_J04500
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display id: component2414862
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access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_B0105
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access: public
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BBa_K1319106
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display id: component2414864
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321340
type: Component
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display id: component2414870
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definition: BBa_K1321340

BBa_K1321005
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BBa_B0105
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BBa_K1321340
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BBa_K1319106
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BBa_K1321122
type: ComponentDefinition
persistent identity: BBa K1321122
display id: BBa_K1321122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt fused to CBDcex
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321122_sequence/1

BBa_K1321003
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display id: component2414991
version: 1
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access: public
definition: BBa_K1321003

BBa_B0105
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access: public
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BBa_K1321091
type: ComponentDefinition
persistent identity: BBa K1321091
display id: BBa_K1321091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDclos
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:34:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PhytoC+CBD4. TBC
mutable notes: PhytoC+CBD4. TBC
mutable provenance: PhytoC+CBD4. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321002
component: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321091_sequence/1

BBa_B0105
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display id: component2414580
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BBa_K1321346
type: ComponentDefinition
persistent identity: BBa K1321346
display id: BBa_K1321346
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:31Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
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component: BBa_K1321337
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321346_sequence/1

BBa_B0105
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access: public
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BBa_K1321002
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display id: component2415121
version: 1
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access: public
definition: BBa_K1321002

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display id: component2415125
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1321337/1

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