//binding/metal
type: Collection
persistent identity: binding metal
display id: binding_metal
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1420004
member: CP47His
member: BBa_K1509000
member: BBa_K1122703
member: BBa_K1460001
member: BBa_K1460006
member: BBa_K1471000
member: PbrR
member: BBa_K519011
member: BBa_K1505001
member: BBa_K2127002
member: BBa_K1420002
member: BBa_K1980011
member: BBa_K1471001
member: BBa_K1550004
member: BBa_K1701000
member: BBa_K1438003
member: Fur
member: BBa_K2127001
member: BBa_K1460003
member: BBa_K1980010
member: BBa_K1505000
member: MymTsfGFP
member: BBa_K1526007
member: BBa_K190019
member: BFRM52H
member: BBa_I721002
member: BBa_K1438002
member: BBa_K1122702
member: BBa_K643002
member: BBa_K1460004
member: BBa_K190021
member: Csp1
member: BBa_K1460005
member: BBa_K1404002
member: BFR
member: BBa_K346001
member: BBa_K1438035
member: BBa_K1505002
member: BBa_K1980012
member: BBa_K1420003
member: BBa_K1420005
member: BBa_K1393000
member: BBa_K346003
member: MBP
member: BBa_K1460002
member: BBa_K1393001
member: BBa_K519010
member: BBa_K643000
member: BBa_K346004
member: BBa_K1460007
member: MymT
member: BBa_K1420001
member: BBa_K1355001
member: BBa_K160001
member: BBa_K205004
member: BBa_K1342003
member: Csp1sfGFP
member: BBa_K190020
member: BBa_K1694006
member: BBa_K1460008
member: BBa_K1355000

BBa_K1342003
type: ComponentDefinition
persistent identity: BBa K1342003
display id: BBa_K1342003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA Part
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-07-23T03:03:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1717_
m user id: 4206
owner id: 22434
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This
mutable notes: soon
mutable provenance: it
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K1342003_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2394007
display id: annotation2394007
version: 1
top level: BBa_K1342003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1342003/annotation2394007/range2394007/1

https://synbiohub.org/public/igem/BBa_K1342003/annotation2394007/range2394007/1
type: Range
persistent identity: range2394007
display id: range2394007
version: 1
direction: direction/forward
top level: BBa_K1342003
start: 1
end: 171
orientation: inline

MBP
type: ComponentDefinition
persistent identity: BBa K231000
display id: BBa_K231000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Metal binding peptide
creator: Daniel R Tarjan
created: 2009-10-20T11:00:00Z
modified: 2015-07-21T02:28:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _328_
m user id: 4206
owner id: 2922
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for a peptide containing a metal binding motif. De novo design allows this peptide to have high affinity for metals. Affinity derives from Cys-Gly repeats. This peptide encodes two Cys-Gly-Cys-Cys-Gly repeats. Other metals besides arsenic, such as Cd and Hg, should be bound as well.
mutable notes: This peptide only contains two repeats of the metal binding motif due to size limitations when ordering oligos. Further repeats of the binding motif may enhance its function.
mutable provenance: Derived from peptide described in: Pazirandeh et al. Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Escherichia coli Expressing a Metal Binding Motif. Applied and Environmental Microbiology, October 1998, p. 4068-4072, Vol. 64, No. 10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K231000_sequence/1

BBa_K1509000
type: ComponentDefinition
persistent identity: BBa K1509000
display id: BBa_K1509000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding for the trans-acting regulator SmtB
creator: Tong Li
created: 2014-09-25T11:00:00Z
modified: 2015-07-23T03:26:04Z
discontinued: false
dominant: false
group u list: _1889_
m user id: 4206
owner id: 24032
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented octanucleotides(5′-GCGATC-GC-3′) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.
mutable notes: According to the mechanisms of smt locus represented above, gene smtB should function with the accompany of smt operator-promoter. Previous cases selected smtA whose product functions by encoding MT-like to sequester metal ions, while our case intended to take advantage of the trigger lies in this locus. By imitating former researches, in which a promoter less lacZ gene was fused upstream of smtA, we respectively splice two selected reporter genes and sequence from smt operator-promoter to smtB, equivalent to replacing smtA with reporter genes, to visualize the effects of metal ions on the locus.
mutable provenance: Metallothioneins(MTs), discovered in horse kidney in 1957 by Margoshes and Vallee are identified as low-molecular weight and sulphydryl rich proteins which bind metal ions in metal-thiolate clusters and whose synthesis increases in response to elevated concentrations of certain metals. In 1988, Olafson and co-workers reported the amino acid sequence of a metal-associated protein isolated from cyanobacterium Synechococcus sp.. With the function of binding metal ions remaining, it only has less than 20% homology in amino acid sequence with known MTs in eukaryotes and thus we call it class II MT or on some occasions MT-like. Owing to the works of Robinson and Huckle, the corresponding locus of Synechococcus PCC7942, smt locus was isolated and analyzed. It includes smtA which encodes the class II MT and a divergently transcribed gene smtB which encodes a trans-acting, autoregulatory repressor that exerts metal ion-inducible negative control over smtA transcription. Between them lies a 100-bp operator-promoter region(smt O-P, BBa_K1509001), which is responsible for the divergent orientation of smtA and smtB.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: smtB
sequence: https://synbiohub.org/public/igem/BBa_K1509000_sequence/1

smtB
type: SequenceAnnotation
persistent identity: annotation2387081
display id: annotation2387081
version: 1
top level: BBa_K1509000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1

https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1
type: Range
persistent identity: range2387081
display id: range2387081
version: 1
direction: direction/forward
top level: BBa_K1509000
start: 1
end: 301
orientation: inline

BBa_K1438002
type: ComponentDefinition
persistent identity: BBa K1438002
display id: BBa_K1438002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hu_Ferritin
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:57Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Human ferritins do loads more iron atoms then bacterial ferritins. Furthermore they are forming metall cores that are less contaminated with phosphates then e.g. bacterial ferritins. Due to this fact, this should result in a purer iron crystal with an improved magnetic moment.
mutable notes: This design was provided by iGEM Calgary and the HuFerritin as a single protein coding biobrick was constructed by iGEM Berlin as this biobrick was not accessible in this version in the registry.
mutable provenance: iGEM Calgary 2013 from a synthesized codon optimized construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438002_sequence/1

BBa_K190020
type: ComponentDefinition
persistent identity: BBa K190020
display id: BBa_K190020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MymT
creator: Nienke Kuipers
created: 2009-08-18T11:00:00Z
modified: 2016-01-13T12:55:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _306_
m user id: 4206
owner id: 4147
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MymT is a methallothionein binding Cu (II)
mutable notes: BioBrick prefix and suffix was added by PCR, also the RBS (B0034) was added by PCR (not in the sequence yet).
mutable provenance: MymT was cloned from pGB68 (kindly provided by Ben Gold, Cornell University), the gene's origin is the genome of ''M. tuberculosis''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: MymT
sequence: https://synbiohub.org/public/igem/BBa_K190020_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2017540
display id: annotation2017540
version: 1
top level: BBa_K190020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190020/annotation2017540/range2017540/1

https://synbiohub.org/public/igem/BBa_K190020/annotation2017540/range2017540/1
type: Range
persistent identity: range2017540
display id: range2017540
version: 1
direction: direction/forward
top level: BBa_K190020
start: 1
end: 18
orientation: inline

MymT
type: SequenceAnnotation
persistent identity: annotation2017541
display id: annotation2017541
version: 1
top level: BBa_K190020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K190020/annotation2017541/range2017541/1

https://synbiohub.org/public/igem/BBa_K190020/annotation2017541/range2017541/1
type: Range
persistent identity: range2017541
display id: range2017541
version: 1
direction: direction/forward
top level: BBa_K190020
start: 19
end: 180
orientation: inline

PbrR
type: ComponentDefinition
persistent identity: BBa K1701001
display id: BBa_K1701001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PbrR
creator: Wei Wei
created: 2015-09-07T11:00:00Z
modified: 2016-01-11T03:17:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2119_
m user id: 4206
owner id: 21319
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 have shown highly sensitive and selective whole-cell detection of lead ions.
mutable notes: none
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/56130720
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1701001_sequence/1

BBa_K1404002
type: ComponentDefinition
persistent identity: BBa K1404002
display id: BBa_K1404002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac-NiCoTB, improves nickel and cobalt internalization
creator: Alexandre Duprey
created: 2014-09-22T11:00:00Z
modified: 2015-07-23T03:01:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 4206
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part comprises a strong E. coli promoter with...
mutable notes: Temp
mutable provenance: The promoter and the rest...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS Paris 2009
sequence annotation: Ptac
sequence annotation: NiCoT
sequence annotation: B0014 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1404002_sequence/1

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2385407
display id: annotation2385407
version: 1
top level: BBa_K1404002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1
type: Range
persistent identity: range2385407
display id: range2385407
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 83
end: 95
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2385405
display id: annotation2385405
version: 1
top level: BBa_K1404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1
type: Range
persistent identity: range2385405
display id: range2385405
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 1
end: 76
orientation: inline

NiCoT
type: SequenceAnnotation
persistent identity: annotation2385406
display id: annotation2385406
version: 1
top level: BBa_K1404002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1
type: Range
persistent identity: range2385406
display id: range2385406
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 96
end: 1212
orientation: inline

B0014 terminator
type: SequenceAnnotation
persistent identity: annotation2385408
display id: annotation2385408
version: 1
top level: BBa_K1404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1
type: Range
persistent identity: range2385408
display id: range2385408
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 1212
end: 1306
orientation: inline

BBa_K1122702
type: ComponentDefinition
persistent identity: BBa K1122702
display id: BBa_K1122702
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric ion-binding protein (FbpA)
creator: Harry Thornton
created: 2013-09-21T11:00:00Z
modified: 2015-07-21T11:42:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18014
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This parts encodes a protein capable of binding ferric ion. The protein includes a signal peptide targeting it to periplasmic space.
mutable notes: none
mutable provenance: ''Neisseria gonorrhoeae'' gDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122702_sequence/1

MymT
type: ComponentDefinition
persistent identity: BBa K1980002
display id: BBa_K1980002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MymT
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T03:09:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. Our version has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
mutable notes: This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
mutable provenance: Mycobacterium tuberculosis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MymT
sequence annotation: Hexahistidine
sequence annotation: Double stop
sequence: https://synbiohub.org/public/igem/BBa_K1980002_sequence/1

MymT
type: SequenceAnnotation
persistent identity: annotation2495239
display id: annotation2495239
version: 1
top level: MymT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980002/annotation2495239/range2495239/1

https://synbiohub.org/public/igem/BBa_K1980002/annotation2495239/range2495239/1
type: Range
persistent identity: range2495239
display id: range2495239
version: 1
direction: direction/forward
top level: MymT
start: 1
end: 159
orientation: inline

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495240
display id: annotation2495240
version: 1
top level: MymT
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980002/annotation2495240/range2495240/1

https://synbiohub.org/public/igem/BBa_K1980002/annotation2495240/range2495240/1
type: Range
persistent identity: range2495240
display id: range2495240
version: 1
direction: direction/forward
top level: MymT
start: 160
end: 177
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495241
display id: annotation2495241
version: 1
top level: MymT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980002/annotation2495241/range2495241/1

https://synbiohub.org/public/igem/BBa_K1980002/annotation2495241/range2495241/1
type: Range
persistent identity: range2495241
display id: range2495241
version: 1
direction: direction/forward
top level: MymT
start: 178
end: 183
orientation: inline

BBa_K1460008
type: ComponentDefinition
persistent identity: BBa K1460008
display id: BBa_K1460008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + CBP4 + pT7 + GST-CRS5 + Ter
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:19:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This composite part functionally codes for the putative lead transporter CBP4 as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport lead ions into the engineered cell where they will be sequestered by the metallothionein allowing for Pb2+ removal from contaminated water.
mutable notes: Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI from Life Technologies).
mutable provenance: This part is a composite of the parts BBa_K1460005 and BBa_K1460001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1460005
component: BBa_K1460001
sequence annotation: BBa_K1460001
sequence annotation: BBa_K1460005
sequence: https://synbiohub.org/public/igem/BBa_K1460008_sequence/1

BBa_K1460005
type: Component
persistent identity: component2381582
display id: component2381582
version: 1
top level: BBa_K1460008
access: public
definition: BBa_K1460005

BBa_K1460001
type: Component
persistent identity: component2381589
display id: component2381589
version: 1
top level: BBa_K1460008
access: public
definition: BBa_K1460001

BBa_K1460001
type: SequenceAnnotation
persistent identity: annotation2381589
display id: annotation2381589
version: 1
top level: BBa_K1460008
location: https://synbiohub.org/public/igem/BBa_K1460008/annotation2381589/range2381589/1
component: BBa_K1460001

https://synbiohub.org/public/igem/BBa_K1460008/annotation2381589/range2381589/1
type: Range
persistent identity: range2381589
display id: range2381589
version: 1
direction: direction/reverse
top level: BBa_K1460008
start: 2198
end: 3225
orientation: inline

BBa_K1460005
type: SequenceAnnotation
persistent identity: annotation2381582
display id: annotation2381582
version: 1
top level: BBa_K1460008
location: https://synbiohub.org/public/igem/BBa_K1460008/annotation2381582/range2381582/1
component: BBa_K1460005

https://synbiohub.org/public/igem/BBa_K1460008/annotation2381582/range2381582/1
type: Range
persistent identity: range2381582
display id: range2381582
version: 1
direction: direction/reverse
top level: BBa_K1460008
start: 1
end: 2189
orientation: inline

BBa_K1505002
type: ComponentDefinition
persistent identity: BBa K1505002
display id: BBa_K1505002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+mntH+mCerulean
creator: Jane Shmushkis, Amey Vrudhula
created: 2014-10-09T11:00:00Z
modified: 2016-01-13T10:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. mntH was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The MntH protein and mCerulean protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of MntH and for better visualization of successful transformation. The original use is to express MntH in Magnetospirillum magneticum strain AMB-1.
mutable notes: We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCerulean protein is expected to improve the folding of MntH in AMB-1.
mutable provenance: RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. MntH is from part BBa_K1505000. mCerulean is from part BBa_K133066.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: mCerulean
sequence annotation: mntH
sequence annotation: ggggs linker
sequence annotation: rbs (from pmsp3)
sequence: https://synbiohub.org/public/igem/BBa_K1505002_sequence/1

mCerulean
type: SequenceAnnotation
persistent identity: annotation2412330
display id: annotation2412330
version: 1
top level: BBa_K1505002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412330/range2412330/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412330/range2412330/1
type: Range
persistent identity: range2412330
display id: range2412330
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 1318
end: 2034
orientation: inline

mntH
type: SequenceAnnotation
persistent identity: annotation2412328
display id: annotation2412328
version: 1
top level: BBa_K1505002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412328/range2412328/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412328/range2412328/1
type: Range
persistent identity: range2412328
display id: range2412328
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 67
end: 1302
orientation: inline

ggggs linker
type: SequenceAnnotation
persistent identity: annotation2412329
display id: annotation2412329
version: 1
top level: BBa_K1505002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412329/range2412329/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412329/range2412329/1
type: Range
persistent identity: range2412329
display id: range2412329
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 1303
end: 1317
orientation: inline

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412327
display id: annotation2412327
version: 1
top level: BBa_K1505002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412327/range2412327/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412327/range2412327/1
type: Range
persistent identity: range2412327
display id: range2412327
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 19
end: 46
orientation: inline

BBa_K1701000
type: ComponentDefinition
persistent identity: BBa K1701000
display id: BBa_K1701000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GolB
creator: Wei Wei
created: 2015-09-07T11:00:00Z
modified: 2016-01-11T03:16:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2119_
m user id: 0
m user id: 4206
owner id: 21319
owning group id: 9
sample status: In stock
status: status/Available
status: status/Planning
bookmark: false
mutable description: Gold-specific binding protein (GolB) is a gold-binding chaperon discovered in S. Typhimurium which has been verified that the display of GolB on E.coli cell surface permitted selective enrichment of gold ions from media containing various metal ions.
mutable notes: None
mutable provenance: http://www.ncbi.nlm.nih.gov/protein/NP_459350.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1701000_sequence/1

BBa_K1460005
type: ComponentDefinition
persistent identity: BBa K1460005
display id: BBa_K1460005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + RBS + CBP4
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:18:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a functional coding sequence for the putative lead and nickel transport protein CBP4 from Nicotiana tabacum. It has been found that expression of this protein leads to increased nickel tolerance and lead intolerance.
mutable notes: The Anderson promoter will provide strong constitutive expression of the CBP4 gene in E. coli.
mutable provenance: Anderson promoter is that of part BBa_J23102. CBP4 is a naturally found coding sequence in Nicotiana tabacum.[1] This part was synthesized by BioBasic and was transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J23102
sequence annotation: CBP4
sequence annotation: BBa_J61101
sequence: https://synbiohub.org/public/igem/BBa_K1460005_sequence/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381551
display id: annotation2381551
version: 1
top level: BBa_K1460005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460005/annotation2381551/range2381551/1

https://synbiohub.org/public/igem/BBa_K1460005/annotation2381551/range2381551/1
type: Range
persistent identity: range2381551
display id: range2381551
version: 1
direction: direction/forward
top level: BBa_K1460005
start: 1
end: 35
orientation: inline

CBP4
type: SequenceAnnotation
persistent identity: annotation2381553
display id: annotation2381553
version: 1
top level: BBa_K1460005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460005/annotation2381553/range2381553/1

https://synbiohub.org/public/igem/BBa_K1460005/annotation2381553/range2381553/1
type: Range
persistent identity: range2381553
display id: range2381553
version: 1
direction: direction/forward
top level: BBa_K1460005
start: 63
end: 2189
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381552
display id: annotation2381552
version: 1
top level: BBa_K1460005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460005/annotation2381552/range2381552/1

https://synbiohub.org/public/igem/BBa_K1460005/annotation2381552/range2381552/1
type: Range
persistent identity: range2381552
display id: range2381552
version: 1
direction: direction/forward
top level: BBa_K1460005
start: 43
end: 56
orientation: inline

BBa_K1438035
type: ComponentDefinition
persistent identity: BBa K1438035
display id: BBa_K1438035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PPMT
creator: Sascha Kaufmann
created: 2014-10-29T12:00:00Z
modified: 2015-07-22T02:15:21Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 22762
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: PPMT
mutable notes: basic part
mutable provenance: Pseudomonas Putida Metallothioneine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K1505000
type: ComponentDefinition
persistent identity: BBa K1505000
display id: BBa_K1505000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+mntH
creator: Renyao Wei
created: 2014-10-08T11:00:00Z
modified: 2016-01-13T10:32:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The gene was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The protein can be used to increase transportation level of divalent metal ions into the cells. Its potential applications include heavy metal bioremediation (by increasing uptake efficiency) and protein expression in cell surface (by fusion with mntH).
mutable notes: It is the coding sequence of MntH
mutable provenance: Azospirillum sp. B510 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs (from pmsp3)
sequence annotation: mntH
sequence: https://synbiohub.org/public/igem/BBa_K1505000_sequence/1

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412322
display id: annotation2412322
version: 1
top level: BBa_K1505000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505000/annotation2412322/range2412322/1

https://synbiohub.org/public/igem/BBa_K1505000/annotation2412322/range2412322/1
type: Range
persistent identity: range2412322
display id: range2412322
version: 1
direction: direction/forward
top level: BBa_K1505000
start: 19
end: 46
orientation: inline

mntH
type: SequenceAnnotation
persistent identity: annotation2412323
display id: annotation2412323
version: 1
top level: BBa_K1505000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505000/annotation2412323/range2412323/1

https://synbiohub.org/public/igem/BBa_K1505000/annotation2412323/range2412323/1
type: Range
persistent identity: range2412323
display id: range2412323
version: 1
direction: direction/forward
top level: BBa_K1505000
start: 67
end: 1302
orientation: inline

BBa_K519011
type: ComponentDefinition
persistent identity: BBa K519011
display id: BBa_K519011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA-Double Term.
creator: Kotone Miyake
created: 2011-09-29T11:00:00Z
modified: 2016-01-13T12:59:12Z
discontinued: false
dominant: true
experience: experience/None
group u list: _684_
m user id: 4206
owner id: 9603
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SmtA is a metallothionein that can bind to metal ions such as Cd(II).
mutable notes: This part is put together with Double terminator.
mutable provenance: Synechococcus sp. PCC7942
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K519010
sequence annotation: BBa_K519010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K519011_sequence/1

BBa_B0015
type: Component
persistent identity: component2147686
display id: component2147686
version: 1
top level: BBa_K519011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K519010
type: Component
persistent identity: component2147679
display id: component2147679
version: 1
top level: BBa_K519011
access: public
definition: BBa_K519010

BBa_K519010
type: SequenceAnnotation
persistent identity: annotation2147679
display id: annotation2147679
version: 1
top level: BBa_K519011
location: https://synbiohub.org/public/igem/BBa_K519011/annotation2147679/range2147679/1
component: BBa_K519010

https://synbiohub.org/public/igem/BBa_K519011/annotation2147679/range2147679/1
type: Range
persistent identity: range2147679
display id: range2147679
version: 1
direction: direction/reverse
top level: BBa_K519011
start: 1
end: 171
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2147686
display id: annotation2147686
version: 1
top level: BBa_K519011
location: https://synbiohub.org/public/igem/BBa_K519011/annotation2147686/range2147686/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K519011/annotation2147686/range2147686/1
type: Range
persistent identity: range2147686
display id: range2147686
version: 1
direction: direction/reverse
top level: BBa_K519011
start: 180
end: 308
orientation: inline

BBa_K160001
type: ComponentDefinition
persistent identity: BBa K160001
display id: BBa_K160001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe-Receptor
creator: RAUL CUERO
created: 2008-10-24T11:00:00Z
modified: 2015-07-20T02:34:02Z
discontinued: false
dominant: false
group u list: _238_
m user id: 4206
owner id: 2946
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Iron binding protein
mutable notes: N/A
mutable provenance: E.coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K160001_sequence/1

Csp1
type: ComponentDefinition
persistent identity: BBa K1980000
display id: BBa_K1980000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAT Copper Storage Protein 1
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-12T02:23:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and a C terminal his tag in an attempt to direct the protein to the periplasm.
mutable notes: Codon optimised for E. coli. TAT sequence changed to an E. coli TAT sequence.
mutable provenance: The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Hexa Histidine
sequence annotation: Csp1
sequence annotation: TAT signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1980000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2491869
display id: annotation2491869
version: 1
top level: Csp1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491869/range2491869/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491869/range2491869/1
type: Range
persistent identity: range2491869
display id: range2491869
version: 1
direction: direction/forward
top level: Csp1
start: 469
end: 474
orientation: inline

Hexa Histidine
type: SequenceAnnotation
persistent identity: annotation2491868
display id: annotation2491868
version: 1
top level: Csp1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491868/range2491868/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491868/range2491868/1
type: Range
persistent identity: range2491868
display id: range2491868
version: 1
direction: direction/forward
top level: Csp1
start: 450
end: 468
orientation: inline

Csp1
type: SequenceAnnotation
persistent identity: annotation2491867
display id: annotation2491867
version: 1
top level: Csp1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491867/range2491867/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491867/range2491867/1
type: Range
persistent identity: range2491867
display id: range2491867
version: 1
direction: direction/forward
top level: Csp1
start: 85
end: 450
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2491866
display id: annotation2491866
version: 1
top level: Csp1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980000/annotation2491866/range2491866/1

https://synbiohub.org/public/igem/BBa_K1980000/annotation2491866/range2491866/1
type: Range
persistent identity: range2491866
display id: range2491866
version: 1
direction: direction/forward
top level: Csp1
start: 1
end: 84
orientation: inline

BBa_K1420004
type: ComponentDefinition
persistent identity: BBa K1420004
display id: BBa_K1420004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merR family transcriptional regulator, regulatory protein for mer operon
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T03:01:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: merR, located upstream of the rest of the mer genes, serves to regulate the operon. The MerR protein contains a C-terminal effector binding region that recognizes ionic mercury. Upon binding it, the protein???s N-terminal helix-turn-helix DNA binding domain can bind the merR promoter region to induce transcription of the operon. The effector binding region of the protein can vary allowing for great diversity in merR-like promoters that can respond to a variety of heavy metals as well as antibiotics and oxidative stress.
mutable notes: N/A
mutable provenance: Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merR
sequence: https://synbiohub.org/public/igem/BBa_K1420004_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2422563
display id: annotation2422563
version: 1
top level: BBa_K1420004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420004/annotation2422563/range2422563/1

https://synbiohub.org/public/igem/BBa_K1420004/annotation2422563/range2422563/1
type: Range
persistent identity: range2422563
display id: range2422563
version: 1
direction: direction/forward
top level: BBa_K1420004
start: 1
end: 435
orientation: inline

Fur
type: ComponentDefinition
persistent identity: BBa K1122666
display id: BBa_K1122666
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric uptake repressor
creator: Hugo Villanueva
created: 2013-07-23T11:00:00Z
modified: 2015-07-24T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The ferric uptake repressor of Bacillus subtilis called YqkL is a regulator that when in contact with iron will bind and repress a regulatory sequence (the Fur box).
mutable notes: We sequenced the Fur box. This can be added in front of genes to provide regulation when in contact with iron.
mutable provenance: This comes from Bacillus subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122666_sequence/1

BBa_K346003
type: ComponentDefinition
persistent identity: BBa K346003
display id: BBa_K346003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0032)+MBP(mercury metal binding peptide engineered from MerR)
creator: Huyang Tengxin
created: 2010-10-13T11:00:00Z
modified: 2015-07-21T02:51:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 6045
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a combination of a rbs, a mbp, coding a mercury metal binding peptide egineered from MerR, and a terminator.
mutable notes: no
mutable provenance: Tn
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0032
sequence annotation: mbp(mercury binding peptide)
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_K346003_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2090100
display id: annotation2090100
version: 1
top level: BBa_K346003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346003/annotation2090100/range2090100/1

https://synbiohub.org/public/igem/BBa_K346003/annotation2090100/range2090100/1
type: Range
persistent identity: range2090100
display id: range2090100
version: 1
direction: direction/forward
top level: BBa_K346003
start: 1
end: 13
orientation: inline

mbp(mercury binding peptide)
type: SequenceAnnotation
persistent identity: annotation2090102
display id: annotation2090102
version: 1
top level: BBa_K346003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346003/annotation2090102/range2090102/1

https://synbiohub.org/public/igem/BBa_K346003/annotation2090102/range2090102/1
type: Range
persistent identity: range2090102
display id: range2090102
version: 1
direction: direction/forward
top level: BBa_K346003
start: 19
end: 342
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2090101
display id: annotation2090101
version: 1
top level: BBa_K346003
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K346003/annotation2090101/range2090101/1

https://synbiohub.org/public/igem/BBa_K346003/annotation2090101/range2090101/1
type: Range
persistent identity: range2090101
display id: range2090101
version: 1
direction: direction/forward
top level: BBa_K346003
start: 5
end: 8
orientation: inline

BBa_K1460004
type: ComponentDefinition
persistent identity: BBa K1460004
display id: BBa_K1460004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + MerT + MerP + ter
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:25:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a functional coding sequence for the mercury transport proteins merT/merP from P. aeruginosa. This part will result in mercury intolerance due to greater mercury transport into engineered cells.
mutable notes: The Anderson promoter provides strong constitutive expression of merT/merP in E. coli
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa. This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J61101
sequence annotation: BBa_J23102
sequence annotation: BBa_B1006
sequence annotation: merP
sequence annotation: merT
sequence: https://synbiohub.org/public/igem/BBa_K1460004_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381483
display id: annotation2381483
version: 1
top level: BBa_K1460004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1
type: Range
persistent identity: range2381483
display id: range2381483
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 43
end: 56
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381484
display id: annotation2381484
version: 1
top level: BBa_K1460004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1
type: Range
persistent identity: range2381484
display id: range2381484
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 1
end: 35
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381487
display id: annotation2381487
version: 1
top level: BBa_K1460004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1
type: Range
persistent identity: range2381487
display id: range2381487
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 702
end: 740
orientation: inline

merP
type: SequenceAnnotation
persistent identity: annotation2381486
display id: annotation2381486
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1
type: Range
persistent identity: range2381486
display id: range2381486
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 426
end: 701
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2381485
display id: annotation2381485
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1
type: Range
persistent identity: range2381485
display id: range2381485
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 63
end: 413
orientation: inline

BBa_K1420002
type: ComponentDefinition
persistent identity: BBa K1420002
display id: BBa_K1420002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merB, Organomercurial Lyase from Serratia marcescens
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:52:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Demethylates organomerury.
mutable notes: N/A
mutable provenance: Amplified from pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merB
sequence: https://synbiohub.org/public/igem/BBa_K1420002_sequence/1

merB
type: SequenceAnnotation
persistent identity: annotation2407616
display id: annotation2407616
version: 1
top level: BBa_K1420002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1

https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1
type: Range
persistent identity: range2407616
display id: range2407616
version: 1
direction: direction/forward
top level: BBa_K1420002
start: 1
end: 639
orientation: inline

BBa_K643000
type: ComponentDefinition
persistent identity: BBa K643000
display id: BBa_K643000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CDS7 cadmium sulfate binding peptide coding sequence
creator: Sung won Lim
created: 2011-09-24T11:00:00Z
modified: 2015-07-21T02:29:26Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _819_
m user id: 4206
owner id: 6460
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This sequence codes for a short peptide that's been reported to bind to cadmium sulfate and nucleate quantum dot formation. (C. Mi et al. / Journal of Biotechnology 153 (2011) 125???132) Peptide sequence: N-GDVHHHGRHGAEHADI-C
mutable notes: Original coding sequence in the referenced paper had NcoI and BamHI sites surrounding the coding sequence for cloning into pET28b IPTG inducible vector. For our purposes we eliminated the restriction sites and instead replaced them with standard biobricking prefix and suffix.
mutable provenance: This part was synthesized de novo based on the information in the original paper. It was PCR'd out of the synthesized plasmid with RFC 23 overhangs and ligated into pSB1C3 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K643000_sequence/1

BBa_K1550004
type: ComponentDefinition
persistent identity: BBa K1550004
display id: BBa_K1550004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BmtA (Lead/Zinc-binding metallothionein from Oscillatoria brevis, codon optimized for E. coli)
creator: Jesse Goldenberg
created: 2014-10-16T11:00:00Z
modified: 2015-08-03T03:05:43Z
discontinued: false
dominant: false
group u list: _1934_
m user id: 4206
owner id: 23749
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the BmtA coding sequence from Oscillatoria brevis, which encodes lead and zinc binding metallothionein (Liu et al., 2003). The BmtA sequence in this part has been codon optimized for E. coli. This part can be used with a constitutive or inducible promoter to produce metallothionein in projects utilizing the metal-binding protein for sequestration purposes.
mutable notes: When used in E. coli for the sequestration of lead or zinc ions in solution, the part may or may not work better in concert with a genomic ZntA knockout. We plan to characterize this combination in the near future.
mutable provenance: The sequence used for the BmtA (metallothionein) was codon optimized for E. coli (using the browser-based software, Gene Design). The native sequence comes from Oscillatoria brevis, and was sourced from an entry in the NCBI database by Liu,T., Nakashima,S., Hirose,K., Uemura,Y., Shibasaka,M., Katsuhara,M. and Kasamo,K. (2003).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1550004_sequence/1

BBa_K1460007
type: ComponentDefinition
persistent identity: BBa K1460007
display id: BBa_K1460007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + merT + merP + Ter + pT7 + GST-CRS5 + Ter
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:19:27Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This composite part functionally codes for the selective mercury transporters merT and merP as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport mercury ions into the engineered cell where they will be sequestered by the metallothionein allowing for Hg2+ removal from contaminated water.
mutable notes: Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI from Life Technologies).
mutable provenance: This part is a composite of parts BBa_K1460004 and BBa_K1460001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1460004
component: BBa_K1460001
sequence annotation: BBa_K1460004
sequence annotation: BBa_K1460001
sequence: https://synbiohub.org/public/igem/BBa_K1460007_sequence/1

BBa_K1460004
type: Component
persistent identity: component2381571
display id: component2381571
version: 1
top level: BBa_K1460007
access: public
definition: BBa_K1460004

BBa_K1460001
type: Component
persistent identity: component2381578
display id: component2381578
version: 1
top level: BBa_K1460007
access: public
definition: BBa_K1460001

BBa_K1460004
type: SequenceAnnotation
persistent identity: annotation2381571
display id: annotation2381571
version: 1
top level: BBa_K1460007
location: https://synbiohub.org/public/igem/BBa_K1460007/annotation2381571/range2381571/1
component: BBa_K1460004

https://synbiohub.org/public/igem/BBa_K1460007/annotation2381571/range2381571/1
type: Range
persistent identity: range2381571
display id: range2381571
version: 1
direction: direction/reverse
top level: BBa_K1460007
start: 1
end: 741
orientation: inline

BBa_K1460001
type: SequenceAnnotation
persistent identity: annotation2381578
display id: annotation2381578
version: 1
top level: BBa_K1460007
location: https://synbiohub.org/public/igem/BBa_K1460007/annotation2381578/range2381578/1
component: BBa_K1460001

https://synbiohub.org/public/igem/BBa_K1460007/annotation2381578/range2381578/1
type: Range
persistent identity: range2381578
display id: range2381578
version: 1
direction: direction/reverse
top level: BBa_K1460007
start: 750
end: 1777
orientation: inline

BFR
type: ComponentDefinition
persistent identity: BBa K1438000
display id: BBa_K1438000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterioferritin (BFR)
creator: Johann Bauerfeind
created: 2014-09-12T11:00:00Z
modified: 2015-07-22T02:12:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Bacterioferritins are the E. coli cells natural iron storage proteins. These hollow nearly spherical protein shells detoxify the cell by sequestering excessive iron and forming Iron(III)hydroxid-oxide particels. Bacterioferritin is an heam containing bacterial ferritin. Each heme is bound in a pocked formed by the interface between a pair of symmetry-related subunits [1]. However, it was investigated that these heme groups may be involved in the release of iron out of the ferritin iron core by forming an heme-mediated electron transfer to reduce immobilized Fe3+ to more soluble Fe2+. [1] Frolow F, Kalb AJ, Yariv J. Structure of a unique twofold symmetric haem-binding site. Nat Struct Biol. 1994 Jul;1(7):453-60. PubMed PMID: 7664064. [2] Yao H, Wang Y, Lovell S, Kumar R, Ruvinsky AM, Battaile KP, Vakser IA, Rivera M. The structure of the BfrB-Bfd complex reveals protein-protein interactions enabling iron release from bacterioferritin. J Am Chem Soc. 2012 Aug 15;134(32):13470-81. doi: 10.1021/ja305180n. Epub 2012 Aug 1. PubMed PMID: 22812654; PubMed Central PMCID: PMC3428730.
mutable notes: For any metal nanoparticles built inside of BFR it would be beneficary to remove any heme groups in order to prevent unloading of BFR by heme-mediated electron transfer.
mutable provenance: Bacterioferritin from E. coli Nissle 1917 genomic DNA. Amplified by using PCR and cloned into pQE80L
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BioBrick
sequence annotation: His Tag
sequence annotation: stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1438000_sequence/1

BioBrick
type: SequenceAnnotation
persistent identity: annotation2398554
display id: annotation2398554
version: 1
top level: BFR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2398554/range2398554/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2398554/range2398554/1
type: Range
persistent identity: range2398554
display id: range2398554
version: 1
direction: direction/forward
top level: BFR
start: 1
end: 513
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2398525
display id: annotation2398525
version: 1
top level: BFR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2398525/range2398525/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2398525/range2398525/1
type: Range
persistent identity: range2398525
display id: range2398525
version: 1
direction: direction/forward
top level: BFR
start: 13
end: 30
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2398526
display id: annotation2398526
version: 1
top level: BFR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2398526/range2398526/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2398526/range2398526/1
type: Range
persistent identity: range2398526
display id: range2398526
version: 1
direction: direction/forward
top level: BFR
start: 511
end: 513
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2383111
display id: annotation2383111
version: 1
top level: BFR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1438000/annotation2383111/range2383111/1

https://synbiohub.org/public/igem/BBa_K1438000/annotation2383111/range2383111/1
type: Range
persistent identity: range2383111
display id: range2383111
version: 1
direction: direction/forward
top level: BFR
start: 1
end: 3
orientation: inline

Csp1sfGFP
type: ComponentDefinition
persistent identity: BBa K1980001
display id: BBa_K1980001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAT Copper Storage Protein 1 sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-12T02:35:29Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker.
mutable notes: Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence.
mutable provenance: The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hexahistidine
sequence annotation: linker
sequence annotation: Double stop
sequence annotation: TAT signal peptide
sequence annotation: sfGFP
sequence annotation: Csp1
sequence: https://synbiohub.org/public/igem/BBa_K1980001_sequence/1

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495231
display id: annotation2495231
version: 1
top level: Csp1sfGFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495231/range2495231/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495231/range2495231/1
type: Range
persistent identity: range2495231
display id: range2495231
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 1180
end: 1197
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2495229
display id: annotation2495229
version: 1
top level: Csp1sfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495229/range2495229/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495229/range2495229/1
type: Range
persistent identity: range2495229
display id: range2495229
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 451
end: 468
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495238
display id: annotation2495238
version: 1
top level: Csp1sfGFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495238/range2495238/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495238/range2495238/1
type: Range
persistent identity: range2495238
display id: range2495238
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 1198
end: 1203
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2495227
display id: annotation2495227
version: 1
top level: Csp1sfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495227/range2495227/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495227/range2495227/1
type: Range
persistent identity: range2495227
display id: range2495227
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 1
end: 84
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2495230
display id: annotation2495230
version: 1
top level: Csp1sfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495230/range2495230/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495230/range2495230/1
type: Range
persistent identity: range2495230
display id: range2495230
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 469
end: 1179
orientation: inline

Csp1
type: SequenceAnnotation
persistent identity: annotation2495228
display id: annotation2495228
version: 1
top level: Csp1sfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980001/annotation2495228/range2495228/1

https://synbiohub.org/public/igem/BBa_K1980001/annotation2495228/range2495228/1
type: Range
persistent identity: range2495228
display id: range2495228
version: 1
direction: direction/forward
top level: Csp1sfGFP
start: 85
end: 450
orientation: inline

BBa_K1980011
type: ComponentDefinition
persistent identity: BBa K1980011
display id: BBa_K1980011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA MymT with divergent expressed CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T03:08:06Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA MymT with plasmid constitutively expressed CueR
mutable notes: stuff
mutable provenance: E. coli TB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980002
component: BBa_K1980006
sequence annotation: BBa_K1980002
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980011_sequence/1

BBa_K1980002
type: Component
persistent identity: component2493044
display id: component2493044
version: 1
top level: BBa_K1980011
access: public
definition: MymT

BBa_K1980006
type: Component
persistent identity: component2493043
display id: component2493043
version: 1
top level: BBa_K1980011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980002
type: SequenceAnnotation
persistent identity: annotation2493044
display id: annotation2493044
version: 1
top level: BBa_K1980011
location: https://synbiohub.org/public/igem/BBa_K1980011/annotation2493044/range2493044/1
component: BBa_K1980002

https://synbiohub.org/public/igem/BBa_K1980011/annotation2493044/range2493044/1
type: Range
persistent identity: range2493044
display id: range2493044
version: 1
direction: direction/reverse
top level: BBa_K1980011
start: 581
end: 763
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493043
display id: annotation2493043
version: 1
top level: BBa_K1980011
location: https://synbiohub.org/public/igem/BBa_K1980011/annotation2493043/range2493043/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980011/annotation2493043/range2493043/1
type: Range
persistent identity: range2493043
display id: range2493043
version: 1
direction: direction/reverse
top level: BBa_K1980011
start: 1
end: 574
orientation: inline

BBa_K1471000
type: ComponentDefinition
persistent identity: BBa K1471000
display id: BBa_K1471000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerE.
creator: Juan No?? Hern??ndez Salazar
created: 2014-10-07T11:00:00Z
modified: 2015-07-22T02:39:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 4206
owner id: 16464
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For mercury bioaccumulation.
mutable notes: We have to optimizes its codons in Arabidopsis Thaliana and removed the restriction sites for EcoR1, Xba1, Spe1 and Pst1.
mutable provenance: Bacterial operon mer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1471000_sequence/1

BBa_K1471001
type: ComponentDefinition
persistent identity: BBa K1471001
display id: BBa_K1471001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerB.
creator: Jaime Antonio del Castillo Nu??ez
created: 2014-10-07T11:00:00Z
modified: 2015-07-22T02:40:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 4206
owner id: 16614
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The MerB gene codes for the proetin which function is to cleave the organic part of the molecule of methyl-mercury
mutable notes: efwgeg
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MerB
sequence: https://synbiohub.org/public/igem/BBa_K1471001_sequence/1

MerB
type: SequenceAnnotation
persistent identity: annotation2419568
display id: annotation2419568
version: 1
top level: BBa_K1471001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1471001/annotation2419568/range2419568/1

https://synbiohub.org/public/igem/BBa_K1471001/annotation2419568/range2419568/1
type: Range
persistent identity: range2419568
display id: range2419568
version: 1
direction: direction/forward
top level: BBa_K1471001
start: 1
end: 639
orientation: inline

BBa_K1460006
type: ComponentDefinition
persistent identity: BBa K1460006
display id: BBa_K1460006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + nixA + Ter + pT7 + GST-CRS5 + Ter
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:18:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This composite part functionally codes for the selective nickel transporter nixA as well as the metallothioniein CRS5 fused to glutathione-S-transferase. This composite part should act to transport nickel ions into the engineered cell where they will be sequestered by the metallothionein allowing for Ni2+ removal from contaminated water.
mutable notes: Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI
mutable provenance: This part is a composite of BBa_K1460003 and BBa_K1460001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1460003
component: BBa_K1460001
sequence annotation: BBa_K1460003
sequence annotation: BBa_K1460001
sequence: https://synbiohub.org/public/igem/BBa_K1460006_sequence/1

BBa_K1460003
type: Component
persistent identity: component2381558
display id: component2381558
version: 1
top level: BBa_K1460006
access: public
definition: BBa_K1460003

BBa_K1460001
type: Component
persistent identity: component2381565
display id: component2381565
version: 1
top level: BBa_K1460006
access: public
definition: BBa_K1460001

BBa_K1460003
type: SequenceAnnotation
persistent identity: annotation2381558
display id: annotation2381558
version: 1
top level: BBa_K1460006
location: https://synbiohub.org/public/igem/BBa_K1460006/annotation2381558/range2381558/1
component: BBa_K1460003

https://synbiohub.org/public/igem/BBa_K1460006/annotation2381558/range2381558/1
type: Range
persistent identity: range2381558
display id: range2381558
version: 1
direction: direction/reverse
top level: BBa_K1460006
start: 1
end: 1029
orientation: inline

BBa_K1460001
type: SequenceAnnotation
persistent identity: annotation2381565
display id: annotation2381565
version: 1
top level: BBa_K1460006
location: https://synbiohub.org/public/igem/BBa_K1460006/annotation2381565/range2381565/1
component: BBa_K1460001

https://synbiohub.org/public/igem/BBa_K1460006/annotation2381565/range2381565/1
type: Range
persistent identity: range2381565
display id: range2381565
version: 1
direction: direction/reverse
top level: BBa_K1460006
start: 1038
end: 2065
orientation: inline

BBa_K1505001
type: ComponentDefinition
persistent identity: BBa K1505001
display id: BBa_K1505001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+smtA+mCherry
creator: Renyao Wei
created: 2014-10-09T11:00:00Z
modified: 2016-01-13T12:56:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. SmtA is a metallithionein isolated from E.coli and it binds covalently to divalent metal cation. It can usually binds 3-4 ions per protein, depending on the ion types. It is often used to increase the accumulation of metal ions intracellularly and potentially improve bacteria's tolerance of metal ions. The SmtA protein and mCherry protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of SmtA and for better visualization of successful transformation. The original use is to express SmtA in Magnetospirillum magneticum strain AMB-1.
mutable notes: We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCherry protein is expected to improve the folding of SmtA (from E.coli) in AMB-1.
mutable provenance: RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. smtA sequence is from BioBrick part BBa_K190021 (we modified the illegal PstI site).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SmtA
sequence annotation: rbs (from pmsp3)
sequence annotation: mCherry
sequence annotation: ggghs linker
sequence: https://synbiohub.org/public/igem/BBa_K1505001_sequence/1

SmtA
type: SequenceAnnotation
persistent identity: annotation2412324
display id: annotation2412324
version: 1
top level: BBa_K1505001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412324/range2412324/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412324/range2412324/1
type: Range
persistent identity: range2412324
display id: range2412324
version: 1
direction: direction/forward
top level: BBa_K1505001
start: 66
end: 233
orientation: inline

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412325
display id: annotation2412325
version: 1
top level: BBa_K1505001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412325/range2412325/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412325/range2412325/1
type: Range
persistent identity: range2412325
display id: range2412325
version: 1
direction: direction/forward
top level: BBa_K1505001
start: 20
end: 47
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2412326
display id: annotation2412326
version: 1
top level: BBa_K1505001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412326/range2412326/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412326/range2412326/1
type: Range
persistent identity: range2412326
display id: range2412326
version: 1
direction: direction/forward
top level: BBa_K1505001
start: 249
end: 959
orientation: inline

ggghs linker
type: SequenceAnnotation
persistent identity: annotation2412331
display id: annotation2412331
version: 1
top level: BBa_K1505001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1505001/annotation2412331/range2412331/1

https://synbiohub.org/public/igem/BBa_K1505001/annotation2412331/range2412331/1
type: Range
persistent identity: range2412331
display id: range2412331
version: 1
direction: direction/forward
top level: BBa_K1505001
start: 234
end: 248
orientation: inline

BBa_K1393001
type: ComponentDefinition
persistent identity: BBa K1393001
display id: BBa_K1393001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OprF(Ala196)+CBP
creator: Jiajun Tan
created: 2014-10-01T11:00:00Z
modified: 2015-07-22T02:48:08Z
discontinued: false
dominant: false
group u list: _1770_
m user id: 4206
owner id: 20256
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality. The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan. Based on the predicted secondary structure and information found in the literature, we chose Ala196 as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
mutable notes: Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
mutable provenance: The OprF gene is from Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBP
sequence annotation: oprF to Ala196
sequence: https://synbiohub.org/public/igem/BBa_K1393001_sequence/1

CBP
type: SequenceAnnotation
persistent identity: annotation2391717
display id: annotation2391717
version: 1
top level: BBa_K1393001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1
type: Range
persistent identity: range2391717
display id: range2391717
version: 1
direction: direction/forward
top level: BBa_K1393001
start: 589
end: 609
orientation: inline

oprF to Ala196
type: SequenceAnnotation
persistent identity: annotation2391711
display id: annotation2391711
version: 1
top level: BBa_K1393001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1
type: Range
persistent identity: range2391711
display id: range2391711
version: 1
direction: direction/forward
top level: BBa_K1393001
start: 1
end: 588
orientation: inline

BBa_K1460002
type: ComponentDefinition
persistent identity: BBa K1460002
display id: BBa_K1460002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:26:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: his part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with an appropriate promoter. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.
mutable notes: The part contains a KpnI cut site between the biobrick prefix and the RBS that is a result of removing the T7 promoter from part BBa_K1460001.
mutable provenance: This part is BBa_K1460001 with the T7 promoter removed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J61101
sequence annotation: GST
sequence annotation: Linker Sequence
sequence annotation: BBa_B1006
sequence annotation: CRS5
sequence: https://synbiohub.org/public/igem/BBa_K1460002_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2380987
display id: annotation2380987
version: 1
top level: BBa_K1460002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1
type: Range
persistent identity: range2380987
display id: range2380987
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 14
end: 27
orientation: inline

GST
type: SequenceAnnotation
persistent identity: annotation2380988
display id: annotation2380988
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1
type: Range
persistent identity: range2380988
display id: range2380988
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 34
end: 687
orientation: inline

Linker Sequence
type: SequenceAnnotation
persistent identity: annotation2380989
display id: annotation2380989
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1
type: Range
persistent identity: range2380989
display id: range2380989
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 688
end: 720
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2380991
display id: annotation2380991
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1
type: Range
persistent identity: range2380991
display id: range2380991
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 931
end: 969
orientation: inline

CRS5
type: SequenceAnnotation
persistent identity: annotation2380990
display id: annotation2380990
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1
type: Range
persistent identity: range2380990
display id: range2380990
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 721
end: 930
orientation: inline

BBa_K1420005
type: ComponentDefinition
persistent identity: BBa K1420005
display id: BBa_K1420005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merT, mercuric transport protein
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T03:01:03Z
discontinued: false
dominant: false
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: merT is found in the Serratia marcescens plasmid pDU1358 on the other side of the bidirectional promoter opposite of merR. It is 351 base pairs long and is hypothesized that it codes for a mercuric transport protein.
mutable notes: N/A
mutable provenance: Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merT
sequence: https://synbiohub.org/public/igem/BBa_K1420005_sequence/1

merT
type: SequenceAnnotation
persistent identity: annotation2407640
display id: annotation2407640
version: 1
top level: BBa_K1420005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420005/annotation2407640/range2407640/1

https://synbiohub.org/public/igem/BBa_K1420005/annotation2407640/range2407640/1
type: Range
persistent identity: range2407640
display id: range2407640
version: 1
direction: direction/forward
top level: BBa_K1420005
start: 1
end: 351
orientation: inline

BBa_K1460003
type: ComponentDefinition
persistent identity: BBa K1460003
display id: BBa_K1460003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + NixA + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:21:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a functional coding sequence for the protein NixA, which is a nickel transporter from H. pylori. This part will result in nickel intolerance from higher levels of intracellular nickel.
mutable notes: The Anderson promoter provides strong constitutive expression of NixA in E. coli.
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B1006
sequence annotation: BBa_J61101
sequence annotation: BBa_J23102
sequence annotation: NixA
sequence: https://synbiohub.org/public/igem/BBa_K1460003_sequence/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381016
display id: annotation2381016
version: 1
top level: BBa_K1460003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1
type: Range
persistent identity: range2381016
display id: range2381016
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 990
end: 1028
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381014
display id: annotation2381014
version: 1
top level: BBa_K1460003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1
type: Range
persistent identity: range2381014
display id: range2381014
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 43
end: 56
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381013
display id: annotation2381013
version: 1
top level: BBa_K1460003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1
type: Range
persistent identity: range2381013
display id: range2381013
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 1
end: 35
orientation: inline

NixA
type: SequenceAnnotation
persistent identity: annotation2381015
display id: annotation2381015
version: 1
top level: BBa_K1460003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1
type: Range
persistent identity: range2381015
display id: range2381015
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 63
end: 989
orientation: inline

BBa_K1526007
type: ComponentDefinition
persistent identity: BBa K1526007
display id: BBa_K1526007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacRS + MntH
creator: Cau?? Westmann
created: 2014-10-06T11:00:00Z
modified: 2015-07-24T12:42:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1908_
m user id: 4206
owner id: 12384
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: lacI + NRAMP
mutable notes: lacI + NRAMP
mutable provenance: lacI + NRAMP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: LacRS
sequence: https://synbiohub.org/public/igem/BBa_K1526007_sequence/1

LacRS
type: SequenceAnnotation
persistent identity: annotation2427896
display id: annotation2427896
version: 1
top level: BBa_K1526007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1526007/annotation2427896/range2427896/1

https://synbiohub.org/public/igem/BBa_K1526007/annotation2427896/range2427896/1
type: Range
persistent identity: range2427896
display id: range2427896
version: 1
direction: direction/forward
top level: BBa_K1526007
start: 1
end: 1558
orientation: inline

BBa_K190021
type: ComponentDefinition
persistent identity: BBa K190021
display id: BBa_K190021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA
creator: Nienke Kuipers
created: 2009-08-18T11:00:00Z
modified: 2016-01-13T12:54:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _306_
m user id: 4206
owner id: 4147
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.
mutable notes: The BioBrick prefix and suffix were added by PCR, also the RBS (B0034) was added.
mutable provenance: The gene was cloned from pET29a-SmtA (kindly provided by Kevin Waldron, Newcastle University), it's origin is from the genome of ''Synechococcus PC''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: SmtA
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K190021_sequence/1

SmtA
type: SequenceAnnotation
persistent identity: annotation2017545
display id: annotation2017545
version: 1
top level: BBa_K190021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K190021/annotation2017545/range2017545/1

https://synbiohub.org/public/igem/BBa_K190021/annotation2017545/range2017545/1
type: Range
persistent identity: range2017545
display id: range2017545
version: 1
direction: direction/forward
top level: BBa_K190021
start: 19
end: 189
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2017544
display id: annotation2017544
version: 1
top level: BBa_K190021
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190021/annotation2017544/range2017544/1

https://synbiohub.org/public/igem/BBa_K190021/annotation2017544/range2017544/1
type: Range
persistent identity: range2017544
display id: range2017544
version: 1
direction: direction/forward
top level: BBa_K190021
start: 1
end: 18
orientation: inline

BBa_K1694006
type: ComponentDefinition
persistent identity: BBa K1694006
display id: BBa_K1694006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gold Binding Polypeptide
creator: CHIH-HSUAN HSU
created: 2015-09-14T11:00:00Z
modified: 2016-01-25T01:09:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2112_
m user id: 4206
owner id: 28523
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Gold binding peptides (GBP) is a kind of peptide which can bind on gold, usually used to immobilize protein on gold surface.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1694006_sequence/1

BBa_K2127001
type: ComponentDefinition
persistent identity: BBa K2127001
display id: BBa_K2127001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible High Expression His-Tagged Photosystem II CP47 subunit
creator: Nafaa Haddou
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:45:18Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 0
m user id: 32415
owner id: 32415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TFBS plac LacO CP47his
mutable notes: synthesized
mutable provenance: Synchocisytys
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_R0010
component: BBa_K2127004
component: BBa_K2127006
component: BBa_B0030
component: BBa_K2127007
sequence annotation: BBa_K2127007
sequence annotation: BBa_K2127004
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127006
sequence: https://synbiohub.org/public/igem/BBa_K2127001_sequence/1

BBa_R0010
type: Component
persistent identity: component2527067
display id: component2527067
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127004
type: Component
persistent identity: component2527081
display id: component2527081
version: 1
top level: BBa_K2127001
access: public
definition: CP47His

BBa_K2127006
type: Component
persistent identity: component2527066
display id: component2527066
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127006/1

BBa_B0030
type: Component
persistent identity: component2527075
display id: component2527075
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K2127007
type: Component
persistent identity: component2527082
display id: component2527082
version: 1
top level: BBa_K2127001
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527082
display id: annotation2527082
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527082/range2527082/1
type: Range
persistent identity: range2527082
display id: range2527082
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1949
end: 2071
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527081
display id: annotation2527081
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527081/range2527081/1
type: Range
persistent identity: range2527081
display id: range2527081
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 401
end: 1948
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527075
display id: annotation2527075
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527075/range2527075/1
type: Range
persistent identity: range2527075
display id: range2527075
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 386
end: 400
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527067
display id: annotation2527067
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527067/range2527067/1
type: Range
persistent identity: range2527067
display id: range2527067
version: 1
direction: direction/forward
top level: BBa_K2127001
start: 186
end: 385
orientation: inline

BBa_K2127006
type: SequenceAnnotation
persistent identity: annotation2527066
display id: annotation2527066
version: 1
top level: BBa_K2127001
location: https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
component: BBa_K2127006

https://synbiohub.org/public/igem/BBa_K2127001/annotation2527066/range2527066/1
type: Range
persistent identity: range2527066
display id: range2527066
version: 1
direction: direction/reverse
top level: BBa_K2127001
start: 1
end: 185
orientation: inline

BBa_K190019
type: ComponentDefinition
persistent identity: BBa K190019
display id: BBa_K190019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fMT
creator: Nienke Kuipers
created: 2009-08-18T11:00:00Z
modified: 2016-01-13T10:21:29Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _306_
m user id: 4206
owner id: 4147
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: fMT is a metallothionein binding Arsenite (III) and Arsenate(V), used to accumulate As when overexpressed. Binds As (III) stronger than As(V) Reference: Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924???2927
mutable notes: Add BioBrick prefix and suffix, add RBS (B0034)
mutable provenance: fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: fMT
sequence annotation: B0034`
sequence: https://synbiohub.org/public/igem/BBa_K190019_sequence/1

fMT
type: SequenceAnnotation
persistent identity: annotation2017543
display id: annotation2017543
version: 1
top level: BBa_K190019
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K190019/annotation2017543/range2017543/1

https://synbiohub.org/public/igem/BBa_K190019/annotation2017543/range2017543/1
type: Range
persistent identity: range2017543
display id: range2017543
version: 1
direction: direction/forward
top level: BBa_K190019
start: 19
end: 222
orientation: inline

B0034`
type: SequenceAnnotation
persistent identity: annotation2017542
display id: annotation2017542
version: 1
top level: BBa_K190019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K190019/annotation2017542/range2017542/1

https://synbiohub.org/public/igem/BBa_K190019/annotation2017542/range2017542/1
type: Range
persistent identity: range2017542
display id: range2017542
version: 1
direction: direction/forward
top level: BBa_K190019
start: 1
end: 18
orientation: inline

BBa_I721002
type: ComponentDefinition
persistent identity: BBa I721002
display id: BBa_I721002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Binding Protein
creator: Jeffrey Hofmann
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lead binding protein part 3
mutable notes: none
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I721002_sequence/1

BBa_K1980012
type: ComponentDefinition
persistent identity: BBa K1980012
display id: BBa_K1980012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA MymT sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 0
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pCopA MymT sfGFP with plasmid constitutively expressed CueR
mutable notes: dsjsdgydahjah
mutable provenance: E. coli TB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980003
component: BBa_K1980006
sequence annotation: BBa_K1980003
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980012_sequence/1

BBa_K1980003
type: Component
persistent identity: component2493046
display id: component2493046
version: 1
top level: BBa_K1980012
access: public
definition: MymTsfGFP

BBa_K1980006
type: Component
persistent identity: component2493045
display id: component2493045
version: 1
top level: BBa_K1980012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980003
type: SequenceAnnotation
persistent identity: annotation2493046
display id: annotation2493046
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
component: BBa_K1980003

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
type: Range
persistent identity: range2493046
display id: range2493046
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 581
end: 1492
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493045
display id: annotation2493045
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
type: Range
persistent identity: range2493045
display id: range2493045
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 1
end: 574
orientation: inline

BBa_K346001
type: ComponentDefinition
persistent identity: BBa K346001
display id: BBa_K346001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (B0034) + MerR (mercury-responsive transcription factor)
creator: Ao Liu & Ying Sheng
created: 2010-10-07T11:00:00Z
modified: 2015-07-21T02:53:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_457_
m user id: 4206
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part was designed as a translational unit for MerR expression.
mutable notes: The native RBS of MerR is not strong enough and its intensity can not be predicted as there is no such a corresponding RBS part in Registry. In order to facilitate the use and further characterization of MerR for future team, we prefixed an RBS part BBa_B0034 from Partsregistry.
mutable provenance: The coding sequence of MerR comes from Tn, prefixed by an RBS part BBa_B0034 from Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: merR
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K346001_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2093295
display id: annotation2093295
version: 1
top level: BBa_K346001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346001/annotation2093295/range2093295/1

https://synbiohub.org/public/igem/BBa_K346001/annotation2093295/range2093295/1
type: Range
persistent identity: range2093295
display id: range2093295
version: 1
direction: direction/forward
top level: BBa_K346001
start: 19
end: 453
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2093291
display id: annotation2093291
version: 1
top level: BBa_K346001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K346001/annotation2093291/range2093291/1

https://synbiohub.org/public/igem/BBa_K346001/annotation2093291/range2093291/1
type: Range
persistent identity: range2093291
display id: range2093291
version: 1
direction: direction/forward
top level: BBa_K346001
start: 1
end: 12
orientation: inline

BBa_K2127002
type: ComponentDefinition
persistent identity: BBa K2127002
display id: BBa_K2127002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP47 His-tagged with Lac Promoter
creator: Dawson Zeng
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:46:10Z
discontinued: false
dominant: false
group u list: _2596_
m user id: 32415
owner id: 17480
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.
mutable notes: Double Stop Codon. ORF Check
mutable provenance: NCBI Reference Sequence: NC_000911.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K2127007
component: BBa_B0030
component: BBa_R0010
component: BBa_K2127004
sequence annotation: BBa_R0010
sequence annotation: BBa_K2127007
sequence annotation: BBa_K2127004
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K2127002_sequence/1

BBa_K2127007
type: Component
persistent identity: component2527114
display id: component2527114
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_K2127007/1

BBa_B0030
type: Component
persistent identity: component2527107
display id: component2527107
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
type: Component
persistent identity: component2527099
display id: component2527099
version: 1
top level: BBa_K2127002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K2127004
type: Component
persistent identity: component2527113
display id: component2527113
version: 1
top level: BBa_K2127002
access: public
definition: CP47His

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2527099
display id: annotation2527099
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527099/range2527099/1
type: Range
persistent identity: range2527099
display id: range2527099
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 1
end: 200
orientation: inline

BBa_K2127007
type: SequenceAnnotation
persistent identity: annotation2527114
display id: annotation2527114
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
component: BBa_K2127007

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527114/range2527114/1
type: Range
persistent identity: range2527114
display id: range2527114
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 1764
end: 1886
orientation: inline

BBa_K2127004
type: SequenceAnnotation
persistent identity: annotation2527113
display id: annotation2527113
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
component: BBa_K2127004

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527113/range2527113/1
type: Range
persistent identity: range2527113
display id: range2527113
version: 1
direction: direction/reverse
top level: BBa_K2127002
start: 216
end: 1763
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2527107
display id: annotation2527107
version: 1
top level: BBa_K2127002
location: https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2127002/annotation2527107/range2527107/1
type: Range
persistent identity: range2527107
display id: range2527107
version: 1
direction: direction/forward
top level: BBa_K2127002
start: 201
end: 215
orientation: inline

BFRM52H
type: ComponentDefinition
persistent identity: BBa K1438001
display id: BBa_K1438001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterioferritin (BFR) M52H heme-deletion
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ATGAAAGGTGATACTAAAGTTATAAATTATCTCAACAAACTGTTGGGAAATGAGCTTGTCGCAATCAATCAGTACTTTCTTCATGCCCGGATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGCACAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTGGAAGGTCTTCCAAACTTACAGGACCTGGGCAAACTGAACATTGGTGAAGATGTTGAGGAAATGCTGCGTTCTGATCTGGCACTTGAGCTGGATGGCGCGAAGAATTTGCGTGAGGCAATTGGTTATGCCGATAGCGTTCATGATTACGTCAGCCGCGATATGATGATAGAAATTTTGCGCGATGAAGAAGGCCATATCGACTGGCTGGAAACGGAACTTGATCTGATTCAGAAGATGGGACTGCAAAATTATCTGCAAGCACAGATTCGCGAAGAAGGTTAA
mutable notes: The M52H Mutagensis leads to an deletion of the BFR heme residue. As a result, BFR will have a reduced iron release rate and keep it's iron content inside of the protein scaffold.
mutable provenance: Escherichia coli Nissle 1917
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438001_sequence/1

BBa_K1420003
type: ComponentDefinition
persistent identity: BBa K1420003
display id: BBa_K1420003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerP, mercuric transport protein periplasmic component
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:53:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: merP is in charge of periplasmic transportation of mercury. This protein has two conserved cysteine residues for specific binding with the mercury ion, Hg(II).
mutable notes: N/A
mutable provenance: Source organism: Serratia marcescens. Plasmid pDU1358 kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merP
sequence: https://synbiohub.org/public/igem/BBa_K1420003_sequence/1

merP
type: SequenceAnnotation
persistent identity: annotation2407638
display id: annotation2407638
version: 1
top level: BBa_K1420003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420003/annotation2407638/range2407638/1

https://synbiohub.org/public/igem/BBa_K1420003/annotation2407638/range2407638/1
type: Range
persistent identity: range2407638
display id: range2407638
version: 1
direction: direction/forward
top level: BBa_K1420003
start: 1
end: 276
orientation: inline

BBa_K1122703
type: ComponentDefinition
persistent identity: BBa K1122703
display id: BBa_K1122703
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric ion-binding protein (FbpA) without a signal peptide
creator: Harry Thornton
created: 2013-09-21T11:00:00Z
modified: 2015-07-21T11:41:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 4206
owner id: 18014
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part encodes a protein capable of binding a ferric ion. The periplasmic signal peptide has been deleted in order to achieve cytoplasmic localisation of the protein.
mutable notes: Deletion of a putative native periplasmic signal peptide
mutable provenance: ''Neisseria gonorrhoeae'' gDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1122703_sequence/1

BBa_K519010
type: ComponentDefinition
persistent identity: BBa K519010
display id: BBa_K519010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA
creator: Kotone Miyake
created: 2011-09-29T11:00:00Z
modified: 2016-01-13T10:24:13Z
discontinued: false
dominant: true
experience: experience/None
group u list: _684_
m user id: 4206
owner id: 9603
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II). This SmtA is cloned from Synechococcus sp. PCC7942 and it has been reported that the cyanobacterial strains that express SmtA can resist medium containing cadmim.
mutable notes: This part was cloned from Synechococcus sp. PCC7942, using primers to add EcoRI, XbaI and SpeI at the ends.
mutable provenance: Synechococcus sp. PCC7942
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K519010_sequence/1

BBa_K1393000
type: ComponentDefinition
persistent identity: BBa K1393000
display id: BBa_K1393000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OprF(Val188)+GS linker+CBP
creator: Jiajun Tan
created: 2014-10-01T11:00:00Z
modified: 2015-07-22T02:47:15Z
discontinued: false
dominant: false
group u list: _1770_
m user id: 4206
owner id: 20256
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality.The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan.
mutable notes: Based on the predicted secondary structure and information found in the literature, we chose Val(188）as potential fusion sites for displaying CBP.CBP is the abbreviation of copper binding peptide made up of seven amino acid.In order to reduce the effect on the CBP activity，we added (G4S) linker between OprF and CBP .
mutable provenance: The OprF gene is from Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBP
sequence annotation: OprF to Val188
sequence: https://synbiohub.org/public/igem/BBa_K1393000_sequence/1

CBP
type: SequenceAnnotation
persistent identity: annotation2392395
display id: annotation2392395
version: 1
top level: BBa_K1393000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1393000/annotation2392395/range2392395/1

https://synbiohub.org/public/igem/BBa_K1393000/annotation2392395/range2392395/1
type: Range
persistent identity: range2392395
display id: range2392395
version: 1
direction: direction/forward
top level: BBa_K1393000
start: 579
end: 600
orientation: inline

OprF to Val188
type: SequenceAnnotation
persistent identity: annotation2392394
display id: annotation2392394
version: 1
top level: BBa_K1393000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1393000/annotation2392394/range2392394/1

https://synbiohub.org/public/igem/BBa_K1393000/annotation2392394/range2392394/1
type: Range
persistent identity: range2392394
display id: range2392394
version: 1
direction: direction/forward
top level: BBa_K1393000
start: 1
end: 564
orientation: inline

BBa_K643002
type: ComponentDefinition
persistent identity: BBa K643002
display id: BBa_K643002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J140 metal binding peptide
creator: Rikki Frenkel, Justin Fabrikant
created: 2011-09-27T11:00:00Z
modified: 2015-07-21T02:29:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _819_
m user id: 4206
owner id: 9151
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: J140, with a sequence of Ser-Leu-Thr-Pro-Leu-Thr-Thr-Ser-His-Leu-Arg-Ser, has been shown to recognize single-crystal CdS and nucleate quantum dots (Mao, C., et al. "Viral assembly of oriented quantum dot nanowires" PNAS 100:6946, 2003)
mutable notes: We wanted to be able to clone this part into the IPTG-inducible expression vector pET28b, so we engineered in restriction sites that would allow us to do so and keep everything in frame. Therefore the part contains an Nco1 site after the Xba1 site, and a BamH1 site before the Spe1 site.
mutable provenance: This protein was selected from a pIII phage display library based on its ability to nucleate ZnS or CdS nanocrystals.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K643002_sequence/1

CP47His
type: ComponentDefinition
persistent identity: BBa K2127004
display id: BBa_K2127004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CP-47-HIS TAG
creator: Mirat Sojitra
created: 2016-10-13T11:00:00Z
modified: 2016-10-21T07:31:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2596_
m user id: 32415
owner id: 32736
owning group id: 9
status: status/Planning
bookmark: false
mutable description: CP-47-HIS TAG
mutable notes: CP-47-HIS TAG
mutable provenance: CP-47-HIS TAG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CP47His
sequence annotation: start
sequence annotation: HisTag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2127004_sequence/1

CP47His
type: SequenceAnnotation
persistent identity: annotation2526360
display id: annotation2526360
version: 1
top level: CP47His
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526360/range2526360/1
type: Range
persistent identity: range2526360
display id: range2526360
version: 1
direction: direction/forward
top level: CP47His
start: 1
end: 1548
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2526359
display id: annotation2526359
version: 1
top level: CP47His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526359/range2526359/1
type: Range
persistent identity: range2526359
display id: range2526359
version: 1
direction: direction/forward
top level: CP47His
start: 1
end: 3
orientation: inline

HisTag
type: SequenceAnnotation
persistent identity: annotation2526361
display id: annotation2526361
version: 1
top level: CP47His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526361/range2526361/1
type: Range
persistent identity: range2526361
display id: range2526361
version: 1
direction: direction/forward
top level: CP47His
start: 1522
end: 1539
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2526362
display id: annotation2526362
version: 1
top level: CP47His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1

https://synbiohub.org/public/igem/BBa_K2127004/annotation2526362/range2526362/1
type: Range
persistent identity: range2526362
display id: range2526362
version: 1
direction: direction/forward
top level: CP47His
start: 1543
end: 1548
orientation: inline

BBa_K1460001
type: ComponentDefinition
persistent identity: BBa K1460001
display id: BBa_K1460001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7 + RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-12-03T10:52:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 24251
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with a strain expressing the bacteriophage T7 polymerase. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.
mutable notes: Flanking the T7 promoter are two KpnI cut sites with both the T7 promoter and a SacI cut site between them. These cut sites are present for easy removal of the T7 promoter for modulation of parts with other promoters. The SacI cut site can be used as a marker for successful removal of the T7 promoter from the part. When digested with SacI, part BBa_K1460001 in pSB1C3 will yield bands of size 2166bp and 931bp. If the T7 promoter is successfully removed, when digested with SacI the plasmid will yield the linearized length of the pSB1C3+BBa_K1460001
mutable provenance: T7 promoter is part BBa_I712074. RBS is from part BBa_J61101. Glutathione-S-transferase is from S. japonicum. CRS5 is a metallothionein from S. cerevisiae. Terminator is part BBa_B1006. This sequence was synthesized by GenScript in the vector pUC57 and was then transferred using traditional cloning into pSB1C3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: CRS5
sequence annotation: BBa_J61101
sequence annotation: Linker Sequence
sequence annotation: BBa_B1006
sequence annotation: GST
sequence annotation: T7 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1460001_sequence/1

CRS5
type: SequenceAnnotation
persistent identity: annotation2380964
display id: annotation2380964
version: 1
top level: BBa_K1460001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380964/range2380964/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380964/range2380964/1
type: Range
persistent identity: range2380964
display id: range2380964
version: 1
direction: direction/forward
top level: BBa_K1460001
start: 779
end: 988
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2380961
display id: annotation2380961
version: 1
top level: BBa_K1460001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380961/range2380961/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380961/range2380961/1
type: Range
persistent identity: range2380961
display id: range2380961
version: 1
direction: direction/forward
top level: BBa_K1460001
start: 72
end: 85
orientation: inline

Linker Sequence
type: SequenceAnnotation
persistent identity: annotation2380963
display id: annotation2380963
version: 1
top level: BBa_K1460001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380963/range2380963/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380963/range2380963/1
type: Range
persistent identity: range2380963
display id: range2380963
version: 1
direction: direction/forward
top level: BBa_K1460001
start: 746
end: 778
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2380965
display id: annotation2380965
version: 1
top level: BBa_K1460001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380965/range2380965/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380965/range2380965/1
type: Range
persistent identity: range2380965
display id: range2380965
version: 1
direction: direction/forward
top level: BBa_K1460001
start: 989
end: 1027
orientation: inline

GST
type: SequenceAnnotation
persistent identity: annotation2380962
display id: annotation2380962
version: 1
top level: BBa_K1460001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380962/range2380962/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380962/range2380962/1
type: Range
persistent identity: range2380962
display id: range2380962
version: 1
direction: direction/forward
top level: BBa_K1460001
start: 92
end: 745
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2380960
display id: annotation2380960
version: 1
top level: BBa_K1460001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460001/annotation2380960/range2380960/1

https://synbiohub.org/public/igem/BBa_K1460001/annotation2380960/range2380960/1
type: Range
persistent identity: range2380960
display id: range2380960
version: 1
direction: direction/forward
top level: BBa_K1460001
start: 13
end: 59
orientation: inline

BBa_K346004
type: ComponentDefinition
persistent identity: BBa K346004
display id: BBa_K346004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS(B0034)_MBP(lead metal binding peptide egineered from PbrR)+Terminator(B0015)
creator: Junyi Jiao
created: 2010-10-13T11:00:00Z
modified: 2016-01-13T10:29:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _457_
m user id: 4206
owner id: 6045
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is combined with a rbs, a mbp which codes for the metal binding peptide and a terminator.
mutable notes: to be given
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: conserved
sequence annotation: BBa_B0015
sequence annotation: mbp(lead binding peptide)
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K346004_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2100339
display id: annotation2100339
version: 1
top level: BBa_K346004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100339/range2100339/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100339/range2100339/1
type: Range
persistent identity: range2100339
display id: range2100339
version: 1
direction: direction/forward
top level: BBa_K346004
start: 5
end: 8
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2100341
display id: annotation2100341
version: 1
top level: BBa_K346004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100341/range2100341/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100341/range2100341/1
type: Range
persistent identity: range2100341
display id: range2100341
version: 1
direction: direction/forward
top level: BBa_K346004
start: 351
end: 479
orientation: inline

mbp(lead binding peptide)
type: SequenceAnnotation
persistent identity: annotation2100340
display id: annotation2100340
version: 1
top level: BBa_K346004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100340/range2100340/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100340/range2100340/1
type: Range
persistent identity: range2100340
display id: range2100340
version: 1
direction: direction/reverse
top level: BBa_K346004
start: 19
end: 345
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2100338
display id: annotation2100338
version: 1
top level: BBa_K346004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K346004/annotation2100338/range2100338/1

https://synbiohub.org/public/igem/BBa_K346004/annotation2100338/range2100338/1
type: Range
persistent identity: range2100338
display id: range2100338
version: 1
direction: direction/forward
top level: BBa_K346004
start: 1
end: 12
orientation: inline

BBa_K1980010
type: ComponentDefinition
persistent identity: BBa K1980010
display id: BBa_K1980010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA TAT Csp1 sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T08:12:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
mutable notes: aahahahah
mutable provenance: E. coli, methanoba trcol OB3b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_K1980001
component: BBa_K1980006
sequence annotation: BBa_K1980001
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980010_sequence/1

BBa_K1980001
type: Component
persistent identity: component2493042
display id: component2493042
version: 1
top level: BBa_K1980010
access: public
definition: Csp1sfGFP

BBa_K1980006
type: Component
persistent identity: component2493041
display id: component2493041
version: 1
top level: BBa_K1980010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980001
type: SequenceAnnotation
persistent identity: annotation2493042
display id: annotation2493042
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
component: BBa_K1980001

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
type: Range
persistent identity: range2493042
display id: range2493042
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 581
end: 1783
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493041
display id: annotation2493041
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
type: Range
persistent identity: range2493041
display id: range2493041
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 1
end: 574
orientation: inline

BBa_K1355001
type: ComponentDefinition
persistent identity: BBa K1355001
display id: BBa_K1355001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulation and transport of mercury ions
creator: Luna Barroco de Lacerda
created: 2014-10-05T11:00:00Z
modified: 2015-07-23T04:05:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 4206
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: We call this part an ???Essential biobrick??? designed to be the key piece of any genetic construction related to mercury, targeting both a biosensor as a bioaccumulative. This biobrick has bidirectional promoter, the regulator MerR with a terminator reverse from the tryptophan operon and a forward MerP and MerT transporters.
mutable notes: If is desirable to accumulate mercury, assemble this biobrick with a good RBS, a coding sequence of a peptide having affinity to metal and a terminator! Its function will be regulated by presence of mercury through MerR regulator. The bacteria will carry the mercury to cytoplasm until it is linked to the peptide having affinity to the metal, making a inactive mercury. Is not it amazing? This is the strategy that we use in our project! The so called ???Essential Biobrick??? was connected to biobrick for bioremediation, bioaccumulation and bio-detection!
mutable provenance: This construction is based on sequences present in the O26-CRL plasmid found in Escherichia coli O26. We added a reverse tryptophan operon terminator to ensure transcription termination of the merR messenger RNA. However we didn???t add transcription terminator after the merP gene, aiming connect this biobrick with others, enabling various functions related to mercury
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Bidirecional promoter
sequence annotation: MerT
sequence annotation: RBS
sequence annotation: MerR
sequence annotation: MerP
sequence annotation: Trp Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1355001_sequence/1

Bidirecional promoter
type: SequenceAnnotation
persistent identity: annotation2415134
display id: annotation2415134
version: 1
top level: BBa_K1355001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415134/range2415134/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415134/range2415134/1
type: Range
persistent identity: range2415134
display id: range2415134
version: 1
direction: direction/reverse
top level: BBa_K1355001
start: 510
end: 538
orientation: inline

MerT
type: SequenceAnnotation
persistent identity: annotation2415141
display id: annotation2415141
version: 1
top level: BBa_K1355001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415141/range2415141/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415141/range2415141/1
type: Range
persistent identity: range2415141
display id: range2415141
version: 1
direction: direction/forward
top level: BBa_K1355001
start: 551
end: 900
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2415752
display id: annotation2415752
version: 1
top level: BBa_K1355001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415752/range2415752/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415752/range2415752/1
type: Range
persistent identity: range2415752
display id: range2415752
version: 1
direction: direction/forward
top level: BBa_K1355001
start: 537
end: 543
orientation: inline

MerR
type: SequenceAnnotation
persistent identity: annotation2415126
display id: annotation2415126
version: 1
top level: BBa_K1355001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415126/range2415126/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415126/range2415126/1
type: Range
persistent identity: range2415126
display id: range2415126
version: 1
direction: direction/reverse
top level: BBa_K1355001
start: 44
end: 480
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2415749
display id: annotation2415749
version: 1
top level: BBa_K1355001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415749/range2415749/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415749/range2415749/1
type: Range
persistent identity: range2415749
display id: range2415749
version: 1
direction: direction/reverse
top level: BBa_K1355001
start: 490
end: 496
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2415143
display id: annotation2415143
version: 1
top level: BBa_K1355001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415143/range2415143/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415143/range2415143/1
type: Range
persistent identity: range2415143
display id: range2415143
version: 1
direction: direction/forward
top level: BBa_K1355001
start: 901
end: 907
orientation: inline

Bidirecional promoter
type: SequenceAnnotation
persistent identity: annotation2415127
display id: annotation2415127
version: 1
top level: BBa_K1355001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415127/range2415127/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415127/range2415127/1
type: Range
persistent identity: range2415127
display id: range2415127
version: 1
direction: direction/forward
top level: BBa_K1355001
start: 486
end: 514
orientation: inline

MerP
type: SequenceAnnotation
persistent identity: annotation2415155
display id: annotation2415155
version: 1
top level: BBa_K1355001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2415155/range2415155/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2415155/range2415155/1
type: Range
persistent identity: range2415155
display id: range2415155
version: 1
direction: direction/forward
top level: BBa_K1355001
start: 914
end: 1189
orientation: inline

Trp Terminator
type: SequenceAnnotation
persistent identity: annotation2397153
display id: annotation2397153
version: 1
top level: BBa_K1355001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1355001/annotation2397153/range2397153/1

https://synbiohub.org/public/igem/BBa_K1355001/annotation2397153/range2397153/1
type: Range
persistent identity: range2397153
display id: range2397153
version: 1
direction: direction/reverse
top level: BBa_K1355001
start: 1
end: 43
orientation: inline

BBa_K1420001
type: ComponentDefinition
persistent identity: BBa K1420001
display id: BBa_K1420001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merA, mercuric reductase from Serratia marcescens
creator: Stephen C. Heinsch
created: 2014-09-25T11:00:00Z
modified: 2015-07-22T02:51:48Z
discontinued: false
dominant: false
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mercuric reductase catalyzes the reduction of Hg to Hg2+.
mutable notes: Mutagenesis of adenine to guanine at position 1026 to remove EcoRI site.
mutable provenance: pDU1358, donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merA
sequence: https://synbiohub.org/public/igem/BBa_K1420001_sequence/1

merA
type: SequenceAnnotation
persistent identity: annotation2387014
display id: annotation2387014
version: 1
top level: BBa_K1420001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420001/annotation2387014/range2387014/1

https://synbiohub.org/public/igem/BBa_K1420001/annotation2387014/range2387014/1
type: Range
persistent identity: range2387014
display id: range2387014
version: 1
direction: direction/forward
top level: BBa_K1420001
start: 1
end: 1686
orientation: inline

BBa_K1438003
type: ComponentDefinition
persistent identity: BBa K1438003
display id: BBa_K1438003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PPMT_GS_ATPCS
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:14:35Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This Fusionprotein was obtained by fusion PPMT and ATPCSand connecting both through the Freiburg GS-Linker PPMT Pseudomonas Putida metallothionein ATPCS Arabidopsis thaliana Phytochelatin synthase
mutable notes: Accodording to he paper Park et al the coexpression of both proteins leads into the formation of an inclusionbody like nanoparticle. Which leads to intercelluar synthesis of diverse nanoparticles.
mutable provenance: Arabidopsis thaliana (Columbia; leave) Pseudomonas Putida KT2240 (synthsized)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438003_sequence/1

BBa_K205004
type: ComponentDefinition
persistent identity: BBa K205004
display id: BBa_K205004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerT - Membranous Mercury transporter
creator: Katelin Haynes
created: 2009-10-19T11:00:00Z
modified: 2016-01-28T12:25:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _311_
m user id: 4206
owner id: 4184
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: MerT is a transporter responsible for the crossing of the inner membrane by mercury. It enhances mercury uptake from the environment.
mutable notes: Standard 23 prefix and suffix was added to the sequence.
mutable provenance: Sequenced sourced from Professor David Wilson, of the Department of Molecular Biology and Genetics, Cornell University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K205004_sequence/1

MymTsfGFP
type: ComponentDefinition
persistent identity: BBa K1980003
display id: BBa_K1980003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MymT sfGFP
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-20T07:26:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.
mutable notes: This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.
mutable provenance: Mycobacterium tuberculosis. Ordered as codon optimised DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hexahistidine
sequence annotation: sfGFP
sequence annotation: Double stop
sequence annotation: linker
sequence annotation: MymT
sequence: https://synbiohub.org/public/igem/BBa_K1980003_sequence/1

Hexahistidine
type: SequenceAnnotation
persistent identity: annotation2495246
display id: annotation2495246
version: 1
top level: MymTsfGFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495246/range2495246/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495246/range2495246/1
type: Range
persistent identity: range2495246
display id: range2495246
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 889
end: 906
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2495245
display id: annotation2495245
version: 1
top level: MymTsfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495245/range2495245/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495245/range2495245/1
type: Range
persistent identity: range2495245
display id: range2495245
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 178
end: 888
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2495248
display id: annotation2495248
version: 1
top level: MymTsfGFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495248/range2495248/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495248/range2495248/1
type: Range
persistent identity: range2495248
display id: range2495248
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 906
end: 912
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2495244
display id: annotation2495244
version: 1
top level: MymTsfGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495244/range2495244/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495244/range2495244/1
type: Range
persistent identity: range2495244
display id: range2495244
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 160
end: 177
orientation: inline

MymT
type: SequenceAnnotation
persistent identity: annotation2495243
display id: annotation2495243
version: 1
top level: MymTsfGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1980003/annotation2495243/range2495243/1

https://synbiohub.org/public/igem/BBa_K1980003/annotation2495243/range2495243/1
type: Range
persistent identity: range2495243
display id: range2495243
version: 1
direction: direction/forward
top level: MymTsfGFP
start: 1
end: 159
orientation: inline

BBa_K1355000
type: ComponentDefinition
persistent identity: BBa K1355000
display id: BBa_K1355000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS + merA (mercuric ion reductase)+ terminator (BBa_ B0015)
creator: Luna Barroco de Lacerda
created: 2014-10-05T11:00:00Z
modified: 2016-01-13T10:34:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 4206
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The MerA gene is one of the most important gene into bacterial mercury-resistance operon. Transforms mercury Hg2+ in Hg0, which is volatile and moves passively out of the bacteria in gaseous form as shown in Figure above. The reduction of Hg2+ occurs by a NADPH-dependent system.
mutable notes: We analyzed all Mercury-resistance (Mer) operon genes, in these following strains available at NCBI: (1) Escherichia coli 042, (2) Escherichia coli O26 , (3) Escherichia coli ACN001, (4) Escherichia coli 838C-R1, (5) Escherichia coli APEC-O103, (6) Escherichia coli UMNK88, (7) Escherichia coli AR060302, (8) Escherichia coli PMV-1. It is showed that the mer genes from strains (1) - (2) - (3) - (4) - (5) is conserved among them. However strains (6) - (7) and (8) did not show any similarity. Therefore we used sequences from (1) to (5) due the consensus in E.coli.
mutable provenance: MerA gene sequence is found in the O26-CRL plasmid from Escherichia coli O26. We also added strong RBS from protein 10 found in phage 17 and the double terminator (BBa_B0015) to ensure efficient termination of transcription and messenger RNA recognition.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: MerA
sequence annotation: rbs
sequence annotation: BBa_ B0015
sequence: https://synbiohub.org/public/igem/BBa_K1355000_sequence/1

MerA
type: SequenceAnnotation
persistent identity: annotation2396893
display id: annotation2396893
version: 1
top level: BBa_K1355000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1355000/annotation2396893/range2396893/1

https://synbiohub.org/public/igem/BBa_K1355000/annotation2396893/range2396893/1
type: Range
persistent identity: range2396893
display id: range2396893
version: 1
direction: direction/forward
top level: BBa_K1355000
start: 24
end: 1639
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2396890
display id: annotation2396890
version: 1
top level: BBa_K1355000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1355000/annotation2396890/range2396890/1

https://synbiohub.org/public/igem/BBa_K1355000/annotation2396890/range2396890/1
type: Range
persistent identity: range2396890
display id: range2396890
version: 1
direction: direction/forward
top level: BBa_K1355000
start: 9
end: 16
orientation: inline

BBa_ B0015
type: SequenceAnnotation
persistent identity: annotation2396891
display id: annotation2396891
version: 1
top level: BBa_K1355000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1355000/annotation2396891/range2396891/1

https://synbiohub.org/public/igem/BBa_K1355000/annotation2396891/range2396891/1
type: Range
persistent identity: range2396891
display id: range2396891
version: 1
direction: direction/forward
top level: BBa_K1355000
start: 1652
end: 1778
orientation: inline