//biosafety/xnase
type: Collection
persistent identity: biosafety xnase
display id: biosafety_xnase
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K526001
member: BBa_K914002
member: BBa_K1172904
member: pLac ColE2
member: ColE2
member: BBa_K1479016
member: test
member: BBa_K150009

//biosafety/semantic_containment
type: Collection
persistent identity: biosafety semantic containment
display id: biosafety_semantic_containment
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K914011
member: test
member: testtest
member: BBa_K914000
member: BBa_K914018
member: BBa_K914012
member: BBa_K914009

//biosafety
type: Collection
persistent identity: biosafety
display id: biosafety
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: nickname
member: BBa_K1554000
member: BBa_K772101
member: BBa_K1554004
member: yobR CDS
member: BBa_K990005
member: BBa_K1184000
member: BBa_K2040122
member: god
member: test
member: BBa_K1720002
member: BBa_K1479006
member: BBa_K748007
member: //biosafety/semantic_containment
member: //biosafety/xnase
member: BBa_K1774000
member: BBa_K1919100
member: T7RSR
member: BBa_K1223001
member: nick
member: //biosafety/kill_switch
member: testtest
member: BBa_K1554005
member: BBa_K1479005

//biosafety/kill_switch
type: Collection
persistent identity: biosafety kill switch
display id: biosafety_kill_switch
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K1172902
member: toxin ParE
member: BBa_K1172909
member: BBa_K879918
member: BBa_K1172912
member: BBa_K879917
member: BBa_K515106
member: BBa_K117000
member: BBa_K1223002
member: BBa_K1639022
member: BBa_K1172901
member: BBa_K515104
member: EndoA
member: BBa_K1919100
member: BBa_K1223001
member: BBa_K1405008
member: BBa_K1172908
member: N-acetyltr
member: CcdB
member: BBa_K1172911
member: BBa_K1184000
member: BBa_K2040122
member: BBa_K628006

BBa_K302033
type: ComponentDefinition
persistent identity: BBa K302033
display id: BBa_K302033
version: 1
was generated by: iGEM to SBOL conversion
description: mazF
created: 2010-10-24T11:00:00Z
modified: 2015-06-01T02:27:54Z
mutable provenance: ''Bacillus Subtilis'' 168 strain
owned by: james
owned by: myers
mutable description: Encodes a stable non-specific ribonuclease in ''Bacillus Subtilis''. It is used in conjungtion with ''mazE'' in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 4206
owner id: 6345
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Sequence isolated based upon the work of Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The ''Bacillus subtilis'' ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. ''Mol Microbiol'' 56: 1139???1148.
star: false
creator: Philip Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double TAA added
sequence: https://synbiohub.org/public/igem/BBa_K302033_sequence/1

double TAA added
type: SequenceAnnotation
persistent identity: annotation2099798
display id: annotation2099798
version: 1
top level: BBa_K302033
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K302033/annotation2099798/range2099798/1

https://synbiohub.org/public/igem/BBa_K302033/annotation2099798/range2099798/1
type: Range
persistent identity: range2099798
display id: range2099798
version: 1
top level: BBa_K302033
direction: direction/forward
start: 334
end: 339
orientation: inline

BBa_K1554005
type: ComponentDefinition
persistent identity: BBa K1554005
display id: BBa_K1554005
version: 1
was generated by: iGEM to SBOL conversion
description: Blue biosafety module for plants
creator: Valencia_UPV team members
created: 2014-09-28T11:00:00Z
modified: 2015-06-09T09:45:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the blue chromoprotein AmilCP, so that plants can be visually differentiated from non-transgenic plants.
mutable notes: The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced.
mutable provenance: TA29 promoter [http://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] Barnase [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] AmilGFP [Waiting for Norwich team part name in registry] P35s [http://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: AmilCP
sequence annotation: T35S
sequence annotation: P35s
sequence annotation: TA29 promoter
sequence annotation: T35s
sequence annotation: barnase
sequence: https://synbiohub.org/public/igem/BBa_K1554005_sequence/1

AmilCP
type: SequenceAnnotation
persistent identity: annotation2389555
display id: annotation2389555
version: 1
top level: BBa_K1554005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389555/range2389555/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389555/range2389555/1
type: Range
persistent identity: range2389555
display id: range2389555
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 2469
end: 3131
orientation: inline

T35S
type: SequenceAnnotation
persistent identity: annotation2389556
display id: annotation2389556
version: 1
top level: BBa_K1554005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389556/range2389556/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389556/range2389556/1
type: Range
persistent identity: range2389556
display id: range2389556
version: 1
direction: direction/reverse
top level: BBa_K1554005
start: 3136
end: 3361
orientation: inline

P35s
type: SequenceAnnotation
persistent identity: annotation2389541
display id: annotation2389541
version: 1
top level: BBa_K1554005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389541/range2389541/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389541/range2389541/1
type: Range
persistent identity: range2389541
display id: range2389541
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 1438
end: 2464
orientation: inline

TA29 promoter
type: SequenceAnnotation
persistent identity: annotation2389538
display id: annotation2389538
version: 1
top level: BBa_K1554005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389538/range2389538/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389538/range2389538/1
type: Range
persistent identity: range2389538
display id: range2389538
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 4
end: 858
orientation: inline

T35s
type: SequenceAnnotation
persistent identity: annotation2389540
display id: annotation2389540
version: 1
top level: BBa_K1554005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389540/range2389540/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389540/range2389540/1
type: Range
persistent identity: range2389540
display id: range2389540
version: 1
direction: direction/reverse
top level: BBa_K1554005
start: 1200
end: 1425
orientation: inline

barnase
type: SequenceAnnotation
persistent identity: annotation2389539
display id: annotation2389539
version: 1
top level: BBa_K1554005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554005/annotation2389539/range2389539/1

https://synbiohub.org/public/igem/BBa_K1554005/annotation2389539/range2389539/1
type: Range
persistent identity: range2389539
display id: range2389539
version: 1
direction: direction/forward
top level: BBa_K1554005
start: 863
end: 1195
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479013
display id: BBa_K1479013
version: 1
was generated by: iGEM to SBOL conversion
description: short short short
creator: designer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long long long long
mutable notes: notes notes notes notes
mutable provenance: source source source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: dist
sequence annotation: lalala
sequence: https://synbiohub.org/public/igem/BBa_K1479013_sequence/1

dist
type: SequenceAnnotation
persistent identity: annotation2399394
display id: annotation2399394
version: 1
top level: nickname
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479013/annotation2399394/range2399394/1

https://synbiohub.org/public/igem/BBa_K1479013/annotation2399394/range2399394/1
type: Range
persistent identity: range2399394
display id: range2399394
version: 1
direction: direction/reverse
top level: nickname
start: 2
end: 3
orientation: inline

dist
type: SequenceAnnotation
persistent identity: annotation2399393
display id: annotation2399393
version: 1
top level: nickname
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1479013/annotation2399393/range2399393/1

https://synbiohub.org/public/igem/BBa_K1479013/annotation2399393/range2399393/1
type: Range
persistent identity: range2399393
display id: range2399393
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 2
orientation: inline

lalala
type: SequenceAnnotation
persistent identity: annotation2399395
display id: annotation2399395
version: 1
top level: nickname
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479013/annotation2399395/range2399395/1

https://synbiohub.org/public/igem/BBa_K1479013/annotation2399395/range2399395/1
type: Range
persistent identity: range2399395
display id: range2399395
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 3
orientation: inline

BBa_K879917
type: ComponentDefinition
persistent identity: BBa K879917
display id: BBa_K879917
version: 1
was generated by: iGEM to SBOL conversion
description: LacO ori
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: lacO ori
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: .
star: false
creator: Thomas Patrick
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: LacO operator
sequence annotation: VR
sequence annotation: RNA II
sequence annotation: BioBrick prefix
sequence annotation: E coli his operon terminator
sequence annotation: pMB1 derived replication origin
sequence annotation: -35
sequence annotation: Chloramphenicol resistance
sequence annotation: VF2
sequence annotation: T0 terminator
sequence annotation: -10
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_K879917_sequence/1

LacO operator
type: SequenceAnnotation
persistent identity: annotation2210567
display id: annotation2210567
version: 1
top level: BBa_K879917
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210567/range2210567/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210567/range2210567/1
type: Range
persistent identity: range2210567
display id: range2210567
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 842
end: 858
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2210377
display id: annotation2210377
version: 1
top level: BBa_K879917
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210377/range2210377/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210377/range2210377/1
type: Range
persistent identity: range2210377
display id: range2210377
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 159
end: 178
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210383
display id: annotation2210383
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210383/range2210383/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210383/range2210383/1
type: Range
persistent identity: range2210383
display id: range2210383
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 277
end: 826
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210342
display id: annotation2210342
version: 1
top level: BBa_K879917
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210342/range2210342/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210342/range2210342/1
type: Range
persistent identity: range2210342
display id: range2210342
version: 1
top level: BBa_K879917
direction: direction/forward
start: 2053
end: 2072
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210376
display id: annotation2210376
version: 1
top level: BBa_K879917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210376/range2210376/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210376/range2210376/1
type: Range
persistent identity: range2210376
display id: range2210376
version: 1
top level: BBa_K879917
direction: direction/forward
start: 22
end: 95
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210378
display id: annotation2210378
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210378/range2210378/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210378/range2210378/1
type: Range
persistent identity: range2210378
display id: range2210378
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 263
end: 877
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2210568
display id: annotation2210568
version: 1
top level: BBa_K879917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210568/range2210568/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210568/range2210568/1
type: Range
persistent identity: range2210568
display id: range2210568
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 859
end: 864
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210380
display id: annotation2210380
version: 1
top level: BBa_K879917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210380/range2210380/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210380/range2210380/1
type: Range
persistent identity: range2210380
display id: range2210380
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 1162
end: 1821
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2210382
display id: annotation2210382
version: 1
top level: BBa_K879917
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210382/range2210382/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210382/range2210382/1
type: Range
persistent identity: range2210382
display id: range2210382
version: 1
top level: BBa_K879917
direction: direction/forward
start: 1935
end: 1954
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210379
display id: annotation2210379
version: 1
top level: BBa_K879917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210379/range2210379/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210379/range2210379/1
type: Range
persistent identity: range2210379
display id: range2210379
version: 1
top level: BBa_K879917
direction: direction/forward
start: 1044
end: 1149
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210566
display id: annotation2210566
version: 1
top level: BBa_K879917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210566/range2210566/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210566/range2210566/1
type: Range
persistent identity: range2210566
display id: range2210566
version: 1
top level: BBa_K879917
direction: direction/reverse
start: 836
end: 841
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210341
display id: annotation2210341
version: 1
top level: BBa_K879917
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210341/range2210341/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210341/range2210341/1
type: Range
persistent identity: range2210341
display id: range2210341
version: 1
top level: BBa_K879917
direction: direction/forward
start: 1
end: 21
orientation: inline

BBa_K2040122
type: ComponentDefinition
persistent identity: BBa K2040122
display id: BBa_K2040122
version: 1
was generated by: iGEM to SBOL conversion
description: KillerRed + NLS
creator: Chilam Poon
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T01:35:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2507_
m user id: 30360
owner id: 30360
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A SV40 nuclear localization signal was added to the phototoxic protein KillerRed.
mutable notes: NLS
mutable provenance: BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SV40 NLS
sequence annotation: KillerRed
sequence: https://synbiohub.org/public/igem/BBa_K2040122_sequence/1

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2502105
display id: annotation2502105
version: 1
top level: BBa_K2040122
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1
type: Range
persistent identity: range2502105
display id: range2502105
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 718
end: 741
orientation: inline

KillerRed
type: SequenceAnnotation
persistent identity: annotation2518117
display id: annotation2518117
version: 1
top level: BBa_K2040122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1
type: Range
persistent identity: range2518117
display id: range2518117
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 1
end: 717
orientation: inline

N-acetyltr
type: ComponentDefinition
persistent identity: BBa K2150103
display id: BBa_K2150103
version: 1
was generated by: iGEM to SBOL conversion
description: 1204 toxin CDS
created: 2016-10-11T11:00:00Z
modified: 2016-10-18T06:28:40Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Zepeng Mu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CDS of N-acetyltransferase GCN5
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2150103_sequence/1

CDS of N-acetyltransferase GCN5
type: SequenceAnnotation
persistent identity: annotation2519779
display id: annotation2519779
version: 1
top level: N-acetyltr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2150103/annotation2519779/range2519779/1

https://synbiohub.org/public/igem/BBa_K2150103/annotation2519779/range2519779/1
type: Range
persistent identity: range2519779
display id: range2519779
version: 1
top level: N-acetyltr
direction: direction/forward
start: 1
end: 501
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2519780
display id: annotation2519780
version: 1
top level: N-acetyltr
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K2150103/annotation2519780/range2519780/1

https://synbiohub.org/public/igem/BBa_K2150103/annotation2519780/range2519780/1
type: Range
persistent identity: range2519780
display id: range2519780
version: 1
top level: N-acetyltr
direction: direction/forward
start: 502
end: 506
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: -35
sequence annotation: -10
sequence annotation: TetR 1
sequence annotation: TetR 2
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

BBa_K914000
type: ComponentDefinition
persistent identity: BBa K914000
display id: BBa_K914000
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-supD-T
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: This part is made from the 3 Biobricks previously describe : R0011 - K228001 - B1006
owned by: james
owned by: myers
mutable description: This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here). This part is used to allow the expression of gene that have one or many amber codon instead of a Serine. Adding IPTG could be usefull when there are to many amber codon in the cell.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The promoter is the last part added, meaning that from the previous intermediate (soon available), we will be able to change the promoter easily.
star: false
creator: Jean Cury
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0011
sequence annotation: modified thr terminator
sequence annotation: lac O1
sequence annotation: BBa_K228001
sequence annotation: BBa_B1006 modified
sequence: https://synbiohub.org/public/igem/BBa_K914000_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2189152
display id: annotation2189152
version: 1
top level: BBa_K914000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189152/range2189152/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189152/range2189152/1
type: Range
persistent identity: range2189152
display id: range2189152
version: 1
top level: BBa_K914000
direction: direction/forward
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2189150
display id: annotation2189150
version: 1
top level: BBa_K914000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189150/range2189150/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189150/range2189150/1
type: Range
persistent identity: range2189150
display id: range2189150
version: 1
top level: BBa_K914000
direction: direction/forward
start: 20
end: 25
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2189148
display id: annotation2189148
version: 1
top level: BBa_K914000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189148/range2189148/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189148/range2189148/1
type: Range
persistent identity: range2189148
display id: range2189148
version: 1
top level: BBa_K914000
direction: direction/forward
start: 1
end: 54
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2189155
display id: annotation2189155
version: 1
top level: BBa_K914000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189155/range2189155/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189155/range2189155/1
type: Range
persistent identity: range2189155
display id: range2189155
version: 1
top level: BBa_K914000
direction: direction/forward
start: 217
end: 238
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2189151
display id: annotation2189151
version: 1
top level: BBa_K914000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189151/range2189151/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189151/range2189151/1
type: Range
persistent identity: range2189151
display id: range2189151
version: 1
top level: BBa_K914000
direction: direction/forward
start: 26
end: 42
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2189153
display id: annotation2189153
version: 1
top level: BBa_K914000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189153/range2189153/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189153/range2189153/1
type: Range
persistent identity: range2189153
display id: range2189153
version: 1
top level: BBa_K914000
direction: direction/forward
start: 64
end: 199
orientation: inline

BBa_B1006 modified
type: SequenceAnnotation
persistent identity: annotation2189154
display id: annotation2189154
version: 1
top level: BBa_K914000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189154/range2189154/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189154/range2189154/1
type: Range
persistent identity: range2189154
display id: range2189154
version: 1
top level: BBa_K914000
direction: direction/forward
start: 208
end: 241
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2189149
display id: annotation2189149
version: 1
top level: BBa_K914000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914000/annotation2189149/range2189149/1

https://synbiohub.org/public/igem/BBa_K914000/annotation2189149/range2189149/1
type: Range
persistent identity: range2189149
display id: range2189149
version: 1
top level: BBa_K914000
direction: direction/forward
start: 3
end: 19
orientation: inline

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: fix
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0062
sequence annotation: prefix
sequence annotation: T
sequence annotation: luxR
sequence annotation: A
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

CcdB
type: ComponentDefinition
persistent identity: BBa K1318000
display id: BBa_K1318000
version: 1
was generated by: iGEM to SBOL conversion
description: CcdB toxin
created: 2014-10-01T11:00:00Z
modified: 2015-07-09T11:28:18Z
mutable provenance: E. coli fertility factor (F plasmid)
owned by: james
owned by: myers
mutable description: CcdB is a toxin that poisons and kills E. coli by inhibiting the DNA gyrase and inducing double strand breaks. CcdB activity can be inhibited by the CcdA antitoxin. This gene is under the control of a galactose inducible promoter
discontinued: true
dominant: false
experience: experience/Works
group u list: _1693_
m user id: 4206
owner id: 20061
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable notes: CcdB is toxic so it has to be expressed in conjunction with CcdA or not on a galactose-containing medium.
star: false
creator: Laurence Van Melderen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CcdB start
sequence annotation: CcdB stop
sequence annotation: CcdB
sequence: https://synbiohub.org/public/igem/BBa_K1318000_sequence/1

CcdB start
type: SequenceAnnotation
persistent identity: annotation2416917
display id: annotation2416917
version: 1
top level: CcdB
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1
type: Range
persistent identity: range2416917
display id: range2416917
version: 1
top level: CcdB
direction: direction/forward
start: 1
end: 3
orientation: inline

CcdB stop
type: SequenceAnnotation
persistent identity: annotation2416918
display id: annotation2416918
version: 1
top level: CcdB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1
type: Range
persistent identity: range2416918
display id: range2416918
version: 1
top level: CcdB
direction: direction/forward
start: 307
end: 309
orientation: inline

CcdB
type: SequenceAnnotation
persistent identity: annotation2416919
display id: annotation2416919
version: 1
top level: CcdB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1
type: Range
persistent identity: range2416919
display id: range2416919
version: 1
top level: CcdB
direction: direction/forward
start: 1
end: 309
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa K1088018
display id: BBa_K1088018
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor from E. coli
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: >gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655 http://ecocyc.org/ECOLI/sequence-rc?type=GENE&object=EG10525
owned by: james
owned by: myers
mutable description: Coding region for the LacI protein. LacI binds to the lac promoter BBa_R0010 and inhibits transcription. IPTG binds LacI and inhibits its function, and thus promotes transcription from the lac promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: GTG start codon has been changed to ATG
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1088018_sequence/1

BBa_K150007
type: ComponentDefinition
persistent identity: BBa K150007
display id: BBa_K150007
version: 1
was generated by: iGEM to SBOL conversion
description: ColicinE1 operon
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: This colicinE1 cassette is derived from the naturally existing colicinE1 plasmid out of the E. coli strain JC411.
owned by: james
owned by: myers
mutable description: This colicinE1 operon contains all three important genes for colicinE1 expression and release. The colE1 gene codes for the lethal colicinE1 protein. The gene for the colE1 immunity protein located on the reverse strand is responsible for the immunity of the producing host bacteria to the colicinE1 and is therefore controlled by a constitutive promoter. Expression of the kil protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE1.
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: colE1 protein
sequence annotation: kil protein
sequence annotation: colE1 immunity protein
sequence: https://synbiohub.org/public/igem/BBa_K150007_sequence/1

colE1 protein
type: SequenceAnnotation
persistent identity: annotation1983358
display id: annotation1983358
version: 1
top level: BBa_K150007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K150007/annotation1983358/range1983358/1

https://synbiohub.org/public/igem/BBa_K150007/annotation1983358/range1983358/1
type: Range
persistent identity: range1983358
display id: range1983358
version: 1
top level: BBa_K150007
direction: direction/forward
start: 1
end: 1569
orientation: inline

kil protein
type: SequenceAnnotation
persistent identity: annotation1983360
display id: annotation1983360
version: 1
top level: BBa_K150007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K150007/annotation1983360/range1983360/1

https://synbiohub.org/public/igem/BBa_K150007/annotation1983360/range1983360/1
type: Range
persistent identity: range1983360
display id: range1983360
version: 1
top level: BBa_K150007
direction: direction/forward
start: 1955
end: 2092
orientation: inline

colE1 immunity protein
type: SequenceAnnotation
persistent identity: annotation1983359
display id: annotation1983359
version: 1
top level: BBa_K150007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K150007/annotation1983359/range1983359/1

https://synbiohub.org/public/igem/BBa_K150007/annotation1983359/range1983359/1
type: Range
persistent identity: range1983359
display id: range1983359
version: 1
top level: BBa_K150007
direction: direction/reverse
start: 1566
end: 1907
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: -10
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_K150005
type: ComponentDefinition
persistent identity: BBa K150005
display id: BBa_K150005
version: 1
was generated by: iGEM to SBOL conversion
description: ribosome binding site of pTrc99a
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: ribosome binding site of pTrc99a
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K150005
sequence: https://synbiohub.org/public/igem/BBa_K150005_sequence/1

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation1983190
display id: annotation1983190
version: 1
top level: BBa_K150005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1

https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
type: Range
persistent identity: range1983190
display id: range1983190
version: 1
top level: BBa_K150005
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K117000
type: ComponentDefinition
persistent identity: BBa K117000
display id: BBa_K117000
version: 1
was generated by: iGEM to SBOL conversion
description: Lysis gene (promotes lysis in colicin-producing bacteria strain)
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
mutable provenance: to be updated
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: to be updated
star: true
creator: Nguyen Xuan Hung
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K117000_sequence/1

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K628006
type: ComponentDefinition
persistent identity: BBa K628006
display id: BBa_K628006
version: 1
was generated by: iGEM to SBOL conversion
description: Protegrin-1 Kill Switch
created: 2011-09-19T11:00:00Z
modified: 2016-02-01T12:54:32Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
group u list: _800_
m user id: 4206
owner id: 9738
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Charles Thompson
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K628000
component: BBa_K206000
component: BBa_B0015
component: BBa_J61101
sequence annotation: BBa_J61101
sequence annotation: BBa_K628000
sequence annotation: BBa_K206000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K628006_sequence/1

BBa_K628000
type: Component
persistent identity: component2135982
display id: component2135982
version: 1
top level: BBa_K628006
access: public
definition: BBa_K628000

BBa_K206000
type: Component
persistent identity: component2135980
display id: component2135980
version: 1
top level: BBa_K628006
access: public
definition: pBAD

BBa_B0015
type: Component
persistent identity: component2135989
display id: component2135989
version: 1
top level: BBa_K628006
access: public
definition: BBa_B0015

BBa_J61101
type: Component
persistent identity: component2135981
display id: component2135981
version: 1
top level: BBa_K628006
access: public
definition: BBa_J61101

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2135981
display id: annotation2135981
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135981/range2135981/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K628006/annotation2135981/range2135981/1
type: Range
persistent identity: range2135981
display id: range2135981
version: 1
top level: BBa_K628006
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K628000
type: SequenceAnnotation
persistent identity: annotation2135982
display id: annotation2135982
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135982/range2135982/1
component: BBa_K628000

https://synbiohub.org/public/igem/BBa_K628006/annotation2135982/range2135982/1
type: Range
persistent identity: range2135982
display id: range2135982
version: 1
top level: BBa_K628006
direction: direction/reverse
start: 157
end: 216
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2135980
display id: annotation2135980
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135980/range2135980/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K628006/annotation2135980/range2135980/1
type: Range
persistent identity: range2135980
display id: range2135980
version: 1
top level: BBa_K628006
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2135989
display id: annotation2135989
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135989/range2135989/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K628006/annotation2135989/range2135989/1
type: Range
persistent identity: range2135989
display id: range2135989
version: 1
top level: BBa_K628006
direction: direction/reverse
start: 225
end: 353
orientation: inline

BBa_K748007
type: ComponentDefinition
persistent identity: BBa K748007
display id: BBa_K748007
version: 1
was generated by: iGEM to SBOL conversion
description: Biofilm formation device. yddV gene with the IPTG inducible promoter.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Gene yddV is under the regulation of Ptrc promoter (IPTG-inducible). yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
mutable notes: nothing special
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_B0034
component: BBa_K748003
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K748003
sequence: https://synbiohub.org/public/igem/BBa_K748007_sequence/1

BBa_R0011
type: Component
persistent identity: component2183395
display id: component2183395
version: 1
top level: BBa_K748007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2183401
display id: component2183401
version: 1
top level: BBa_K748007
access: public
definition: BBa_B0034

BBa_K748003
type: Component
persistent identity: component2183402
display id: component2183402
version: 1
top level: BBa_K748007
access: public
definition: https://synbiohub.org/public/igem/BBa_K748003/1

BBa_B0015
type: Component
persistent identity: component2183409
display id: component2183409
version: 1
top level: BBa_K748007
access: public
definition: BBa_B0015

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2183409
display id: annotation2183409
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183409/range2183409/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K748007/annotation2183409/range2183409/1
type: Range
persistent identity: range2183409
display id: range2183409
version: 1
direction: direction/reverse
top level: BBa_K748007
start: 1476
end: 1604
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2183395
display id: annotation2183395
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183395/range2183395/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K748007/annotation2183395/range2183395/1
type: Range
persistent identity: range2183395
display id: range2183395
version: 1
direction: direction/forward
top level: BBa_K748007
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183401
display id: annotation2183401
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183401/range2183401/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K748007/annotation2183401/range2183401/1
type: Range
persistent identity: range2183401
display id: range2183401
version: 1
direction: direction/reverse
top level: BBa_K748007
start: 64
end: 75
orientation: inline

BBa_K748003
type: SequenceAnnotation
persistent identity: annotation2183402
display id: annotation2183402
version: 1
top level: BBa_K748007
location: https://synbiohub.org/public/igem/BBa_K748007/annotation2183402/range2183402/1
component: BBa_K748003

https://synbiohub.org/public/igem/BBa_K748007/annotation2183402/range2183402/1
type: Range
persistent identity: range2183402
display id: range2183402
version: 1
direction: direction/reverse
top level: BBa_K748007
start: 82
end: 1467
orientation: inline

BBa_K150107
type: ComponentDefinition
persistent identity: BBa K150107
display id: BBa_K150107
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD07
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: linkerHD07
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150107_sequence/1

BBa_K1479016
type: ComponentDefinition
persistent identity: BBa K1479016
display id: BBa_K1479016
version: 1
was generated by: iGEM to SBOL conversion
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: adsfsdfasdfasdf
owned by: james
owned by: myers
mutable description: fsadfasdfasdfasfdasdfa
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable notes: sadfasdfadfsdfa
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: 1
sequence annotation: 4

1
type: SequenceAnnotation
persistent identity: annotation2418316
display id: annotation2418316
version: 1
top level: BBa_K1479016
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418316/range2418316/1
type: Range
persistent identity: range2418316
display id: range2418316
version: 1
top level: BBa_K1479016
direction: direction/forward
start: 2
end: 3
orientation: inline

4
type: SequenceAnnotation
persistent identity: annotation2418317
display id: annotation2418317
version: 1
top level: BBa_K1479016
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1

https://synbiohub.org/public/igem/BBa_K1479016/annotation2418317/range2418317/1
type: Range
persistent identity: range2418317
display id: range2418317
version: 1
top level: BBa_K1479016
direction: direction/forward
start: 5
end: 6
orientation: inline

yobR CDS
type: ComponentDefinition
persistent identity: BBa K2150102
display id: BBa_K2150102
version: 1
was generated by: iGEM to SBOL conversion
description: 136 toxin (yobR) CDS
creator: Zepeng Mu
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T11:44:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2150102_sequence/1

BBa_K515004
type: ComponentDefinition
persistent identity: BBa K515004
display id: BBa_K515004
version: 1
was generated by: iGEM to SBOL conversion
description: T4 Anti-Holin
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: genomics
owned by: james
owned by: myers
mutable description: An antitoxin sequence for holin
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Atipat Patharagulpong
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: antiholin
sequence annotation: insulator
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K515004_sequence/1

antiholin
type: SequenceAnnotation
persistent identity: annotation2129768
display id: annotation2129768
version: 1
top level: BBa_K515004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K515004/annotation2129768/range2129768/1

https://synbiohub.org/public/igem/BBa_K515004/annotation2129768/range2129768/1
type: Range
persistent identity: range2129768
display id: range2129768
version: 1
top level: BBa_K515004
direction: direction/forward
start: 36
end: 332
orientation: inline

insulator
type: SequenceAnnotation
persistent identity: annotation2129766
display id: annotation2129766
version: 1
top level: BBa_K515004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K515004/annotation2129766/range2129766/1

https://synbiohub.org/public/igem/BBa_K515004/annotation2129766/range2129766/1
type: Range
persistent identity: range2129766
display id: range2129766
version: 1
top level: BBa_K515004
direction: direction/forward
start: 1
end: 15
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2129767
display id: annotation2129767
version: 1
top level: BBa_K515004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K515004/annotation2129767/range2129767/1

https://synbiohub.org/public/igem/BBa_K515004/annotation2129767/range2129767/1
type: Range
persistent identity: range2129767
display id: range2129767
version: 1
top level: BBa_K515004
direction: direction/forward
start: 16
end: 35
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: mrfp1
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: cI lambda
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

pLac ColE2
type: ComponentDefinition
persistent identity: BBa K914001
display id: BBa_K914001
version: 1
was generated by: iGEM to SBOL conversion
description: pLac Colicin E2 Immunity protein
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: pDEW Colicin E2
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Colicin E2 Immunity protein under pLac promoter (BBa_R0011) with repressilator RBS. This part can be used to confer immunity against the colicin E2 activity protein by induction with IPTG.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13463
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We selected the repressilator RBS and pLac to drive production of Colicin E2 Immunity protein
star: false
creator: Julianne Rieders
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K914002
sequence annotation: -10
sequence annotation: -35
sequence annotation: repressilator
sequence annotation: cei
sequence annotation: lac O1
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K914001_sequence/1

BBa_K914002
type: SequenceAnnotation
persistent identity: annotation2188659
display id: annotation2188659
version: 1
top level: pLac ColE2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188659/range2188659/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188659/range2188659/1
type: Range
persistent identity: range2188659
display id: range2188659
version: 1
top level: pLac ColE2
direction: direction/reverse
start: 64
end: 345
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2188657
display id: annotation2188657
version: 1
top level: pLac ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188657/range2188657/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188657/range2188657/1
type: Range
persistent identity: range2188657
display id: range2188657
version: 1
top level: pLac ColE2
direction: direction/forward
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2188655
display id: annotation2188655
version: 1
top level: pLac ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188655/range2188655/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188655/range2188655/1
type: Range
persistent identity: range2188655
display id: range2188655
version: 1
top level: pLac ColE2
direction: direction/forward
start: 20
end: 25
orientation: inline

repressilator
type: SequenceAnnotation
persistent identity: annotation2188658
display id: annotation2188658
version: 1
top level: pLac ColE2
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188658/range2188658/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188658/range2188658/1
type: Range
persistent identity: range2188658
display id: range2188658
version: 1
top level: pLac ColE2
direction: direction/forward
start: 64
end: 84
orientation: inline

cei
type: SequenceAnnotation
persistent identity: annotation2188660
display id: annotation2188660
version: 1
top level: pLac ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188660/range2188660/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188660/range2188660/1
type: Range
persistent identity: range2188660
display id: range2188660
version: 1
top level: pLac ColE2
direction: direction/forward
start: 85
end: 345
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2188654
display id: annotation2188654
version: 1
top level: pLac ColE2
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188654/range2188654/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188654/range2188654/1
type: Range
persistent identity: range2188654
display id: range2188654
version: 1
top level: pLac ColE2
direction: direction/forward
start: 3
end: 19
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2188653
display id: annotation2188653
version: 1
top level: pLac ColE2
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188653/range2188653/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188653/range2188653/1
type: Range
persistent identity: range2188653
display id: range2188653
version: 1
top level: pLac ColE2
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2188656
display id: annotation2188656
version: 1
top level: pLac ColE2
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K914001/annotation2188656/range2188656/1

https://synbiohub.org/public/igem/BBa_K914001/annotation2188656/range2188656/1
type: Range
persistent identity: range2188656
display id: range2188656
version: 1
top level: pLac ColE2
direction: direction/forward
start: 26
end: 42
orientation: inline

EndoA
type: ComponentDefinition
persistent identity: BBa K2150100
display id: BBa_K2150100
version: 1
was generated by: iGEM to SBOL conversion
description: 134 toxin CDS
created: 2016-10-11T11:00:00Z
modified: 2016-10-13T09:53:36Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: true
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: a
star: false
creator: Zepeng Mu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2150100_sequence/1

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_K1405008
type: ComponentDefinition
persistent identity: BBa K1405008
display id: BBa_K1405008
version: 1
was generated by: iGEM to SBOL conversion
description: A Kill Switch with "memory" time repressed by IPTG
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:17Z
mutable provenance: Name Number Description CI coding part BBa-C0051 Coding cI Promoter 1 BBa-J23116 Weak constitutive promoter Promoter 2 BBa-R0010 LacI repressible promoter Promoter 3 BBa-R0051 CI repressible promoter Table 1 Biobricks used in the design
owned by: james
owned by: myers
mutable description: We plan to establish a new E.Coli strain without lacI and MazEF system gene to avoid the inference of cell itself. We will test cI background expression level to see if we need to knock out cI. As is shown in Fig.1, Fig.2, Fig.3 and Fig.4, in the ???Stable State???, before poured into the soil, the E. Coli fertilizer is cultured in the medium with IPTG. Promoter 1 is a weak constitutive promoter, so lacI is transcribed and translated at a considerate low level. Therefore, high concentration of IPTG, which binds LacI and changes LacI???s conformation, is able to inactive LacI and open the promoter 2. Then, CI can be highly expressed to repress promoter3. Finally, mazF is inhibited. After fertilizing, no more IPTG exists in the soil to bind LacI. As a result, LacI with a tag which stabilize the protein takes time to accumulate to a certain high concentration, which is the repression threshold of promoter 2, aiming to represses promoter 2 as well as the expression of cI. The time needed to finish this progress is the designed ???memory time???. During this process, E. coli takes its own responsibility to deliver Mo. And after that, it can be killed to reduce the pollution to environment. As is shown in table 1, all the biobricks in the design are from the top 10 most used parts of iGEM to guarantee the feasibility. Name Number Description CI coding part BBa-C0051 Coding cI Promoter 1 BBa-J23116 Weak constitutive promoter Promoter 2 BBa-R0010 LacI repressible promoter Promoter 3 BBa-R0051 CI repressible promoter Table 1 Biobricks used in the design In the future, we plan to model the ???memory time??? and do more wet-experiments to confirm its feasibility. As for the modeling, we will test the expression level of promoter 1, the minimal stand of LacI to repress promoter 2 and the degradation speed of LacI. On the other hand, we will test the background expression of CI after removing IPTG to check if the CI concentration is low enough to open the promoter 3. Then we wil…
discontinued: false
dominant: false
experience: experience/None
group u list: _1783_
m user id: 0
owner id: 20576
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 1
star: false
creator: Xiaoyu Yu
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23116
component: BBa_K1088018
component: BBa_R0010
component: BBa_K302033
component: BBa_B0015
component: BBa_C0051
component: BBa_R0051
sequence annotation: BBa_K302033
sequence annotation: BBa_K1088018
sequence annotation: BBa_R0051
sequence annotation: BBa_B0015
sequence annotation: BBa_C0051
sequence annotation: BBa_R0010
sequence annotation: BBa_J23116
sequence: https://synbiohub.org/public/igem/BBa_K1405008_sequence/1

BBa_J23116
type: Component
persistent identity: component2420276
display id: component2420276
version: 1
top level: BBa_K1405008
access: public
definition: BBa_J23116

BBa_K1088018
type: Component
persistent identity: component2420277
display id: component2420277
version: 1
top level: BBa_K1405008
access: public
definition: lacI

BBa_R0010
type: Component
persistent identity: component2420285
display id: component2420285
version: 1
top level: BBa_K1405008
access: public
definition: LacI

BBa_K302033
type: Component
persistent identity: component2420309
display id: component2420309
version: 1
top level: BBa_K1405008
access: public
definition: BBa_K302033

BBa_B0015
type: Component
persistent identity: component2420316
display id: component2420316
version: 1
top level: BBa_K1405008
access: public
definition: BBa_B0015

BBa_B0015
type: Component
persistent identity: component2420284
display id: component2420284
version: 1
top level: BBa_K1405008
access: public
definition: BBa_B0015

BBa_C0051
type: Component
persistent identity: component2420295
display id: component2420295
version: 1
top level: BBa_K1405008
access: public
definition: cI lam

BBa_B0015
type: Component
persistent identity: component2420302
display id: component2420302
version: 1
top level: BBa_K1405008
access: public
definition: BBa_B0015

BBa_R0051
type: Component
persistent identity: component2420304
display id: component2420304
version: 1
top level: BBa_K1405008
access: public
definition: cI lam

BBa_K302033
type: SequenceAnnotation
persistent identity: annotation2420309
display id: annotation2420309
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420309/range2420309/1
component: BBa_K302033

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420309/range2420309/1
type: Range
persistent identity: range2420309
display id: range2420309
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 2451
end: 2789
orientation: inline

BBa_K1088018
type: SequenceAnnotation
persistent identity: annotation2420277
display id: annotation2420277
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420277/range2420277/1
component: BBa_K1088018

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420277/range2420277/1
type: Range
persistent identity: range2420277
display id: range2420277
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 42
end: 1124
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2420304
display id: annotation2420304
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420304/range2420304/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420304/range2420304/1
type: Range
persistent identity: range2420304
display id: range2420304
version: 1
top level: BBa_K1405008
direction: direction/forward
start: 2396
end: 2444
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2420316
display id: annotation2420316
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420316/range2420316/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420316/range2420316/1
type: Range
persistent identity: range2420316
display id: range2420316
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 2798
end: 2926
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2420284
display id: annotation2420284
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420284/range2420284/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420284/range2420284/1
type: Range
persistent identity: range2420284
display id: range2420284
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 1133
end: 1261
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2420302
display id: annotation2420302
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420302/range2420302/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420302/range2420302/1
type: Range
persistent identity: range2420302
display id: range2420302
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 2259
end: 2387
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2420295
display id: annotation2420295
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420295/range2420295/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420295/range2420295/1
type: Range
persistent identity: range2420295
display id: range2420295
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 1476
end: 2250
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2420285
display id: annotation2420285
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420285/range2420285/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420285/range2420285/1
type: Range
persistent identity: range2420285
display id: range2420285
version: 1
top level: BBa_K1405008
direction: direction/forward
start: 1270
end: 1469
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2420276
display id: annotation2420276
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420276/range2420276/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420276/range2420276/1
type: Range
persistent identity: range2420276
display id: range2420276
version: 1
top level: BBa_K1405008
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1479005
type: ComponentDefinition
persistent identity: BBa K1479005
display id: BBa_K1479005
version: 1
was generated by: iGEM to SBOL conversion
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: cds
sequence annotation: 1
sequence annotation: st
sequence: https://synbiohub.org/public/igem/BBa_K1479005_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2417782
display id: annotation2417782
version: 1
top level: BBa_K1479005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417782/range2417782/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417782/range2417782/1
type: Range
persistent identity: range2417782
display id: range2417782
version: 1
direction: direction/forward
top level: BBa_K1479005
start: 3
end: 5
orientation: inline

1
type: SequenceAnnotation
persistent identity: annotation2417781
display id: annotation2417781
version: 1
top level: BBa_K1479005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417781/range2417781/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417781/range2417781/1
type: Range
persistent identity: range2417781
display id: range2417781
version: 1
direction: direction/forward
top level: BBa_K1479005
start: 1
end: 2
orientation: inline

st
type: SequenceAnnotation
persistent identity: annotation2417783
display id: annotation2417783
version: 1
top level: BBa_K1479005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479005/annotation2417783/range2417783/1

https://synbiohub.org/public/igem/BBa_K1479005/annotation2417783/range2417783/1
type: Range
persistent identity: range2417783
display id: range2417783
version: 1
direction: direction/reverse
top level: BBa_K1479005
start: 6
end: 7
orientation: inline

BBa_K1184000
type: ComponentDefinition
persistent identity: BBa K1184000
display id: BBa_K1184000
version: 1
was generated by: iGEM to SBOL conversion
description: KillerRed
creator: Eric Pederson
created: 2013-09-03T11:00:00Z
modified: 2016-01-25T02:40:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1497_
m user id: 4206
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates. This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
mutable notes: This sequence is not codon optimized for E. coli.
mutable provenance: Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Phototoxic Mutation Asn(147)
sequence annotation: Phototoxic Mutation Glu(70)
sequence annotation: Chromophore
sequence annotation: Phototoxic Mutation Ser(121)
sequence annotation: Double Stop
sequence annotation: Start
sequence annotation: Phototoxic Mutation Ala(163)
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1184000_sequence/1

Phototoxic Mutation Asn(147)
type: SequenceAnnotation
persistent identity: annotation2334960
display id: annotation2334960
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1
type: Range
persistent identity: range2334960
display id: range2334960
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 439
end: 441
orientation: inline

Phototoxic Mutation Glu(70)
type: SequenceAnnotation
persistent identity: annotation2334956
display id: annotation2334956
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1
type: Range
persistent identity: range2334956
display id: range2334956
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 208
end: 210
orientation: inline

Chromophore
type: SequenceAnnotation
persistent identity: annotation2334958
display id: annotation2334958
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1
type: Range
persistent identity: range2334958
display id: range2334958
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 199
end: 207
orientation: inline

Phototoxic Mutation Ser(121)
type: SequenceAnnotation
persistent identity: annotation2334957
display id: annotation2334957
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1
type: Range
persistent identity: range2334957
display id: range2334957
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 367
end: 369
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2334953
display id: annotation2334953
version: 1
top level: BBa_K1184000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1
type: Range
persistent identity: range2334953
display id: range2334953
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 718
end: 723
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2334954
display id: annotation2334954
version: 1
top level: BBa_K1184000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1
type: Range
persistent identity: range2334954
display id: range2334954
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 3
orientation: inline

Phototoxic Mutation Ala(163)
type: SequenceAnnotation
persistent identity: annotation2334965
display id: annotation2334965
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1
type: Range
persistent identity: range2334965
display id: range2334965
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 487
end: 489
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2334955
display id: annotation2334955
version: 1
top level: BBa_K1184000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1
type: Range
persistent identity: range2334955
display id: range2334955
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 717
orientation: inline

ColE2
type: ComponentDefinition
persistent identity: BBa K131000
display id: BBa_K131000
version: 1
was generated by: iGEM to SBOL conversion
description: ColE2 Operon
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:47Z
mutable provenance: This part was cloned from a plasmid containing the operon which was received from UC Berkeley.
owned by: james
owned by: myers
mutable description: This part contains the ColE2 operon in its entirety.
discontinued: false
dominant: false
experience: experience/Works
group u list: _259_
m user id: 0
owner id: 1917
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None?
star: true
creator: Kevin McLeod
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K131003
sequence annotation: -15 Signal
sequence annotation: SOS Box
sequence annotation: BBa_K131008
sequence annotation: Region of Dyad Symmetry
sequence annotation: BBa_K131005
sequence annotation: BBa_K131001
sequence annotation: BBa_K131004
sequence annotation: CeaB
sequence annotation: CeiB
sequence annotation: CelB
sequence annotation: BBa_K131007
sequence annotation: -35 Signal
sequence: https://synbiohub.org/public/igem/BBa_K131000_sequence/1

BBa_K131003
type: SequenceAnnotation
persistent identity: annotation1968212
display id: annotation1968212
version: 1
top level: ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968212/range1968212/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968212/range1968212/1
type: Range
persistent identity: range1968212
display id: range1968212
version: 1
top level: ColE2
direction: direction/forward
start: 2079
end: 2339
orientation: inline

-15 Signal
type: SequenceAnnotation
persistent identity: annotation1968208
display id: annotation1968208
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968208/range1968208/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968208/range1968208/1
type: Range
persistent identity: range1968208
display id: range1968208
version: 1
top level: ColE2
direction: direction/forward
start: 241
end: 246
orientation: inline

SOS Box
type: SequenceAnnotation
persistent identity: annotation1968209
display id: annotation1968209
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968209/range1968209/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968209/range1968209/1
type: Range
persistent identity: range1968209
display id: range1968209
version: 1
top level: ColE2
direction: direction/forward
start: 252
end: 267
orientation: inline

BBa_K131008
type: SequenceAnnotation
persistent identity: annotation1968215
display id: annotation1968215
version: 1
top level: ColE2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968215/range1968215/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968215/range1968215/1
type: Range
persistent identity: range1968215
display id: range1968215
version: 1
top level: ColE2
direction: direction/forward
start: 2565
end: 2603
orientation: inline

Region of Dyad Symmetry
type: SequenceAnnotation
persistent identity: annotation1968206
display id: annotation1968206
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968206/range1968206/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968206/range1968206/1
type: Range
persistent identity: range1968206
display id: range1968206
version: 1
top level: ColE2
direction: direction/forward
start: 136
end: 161
orientation: inline

BBa_K131005
type: SequenceAnnotation
persistent identity: annotation1968214
display id: annotation1968214
version: 1
top level: ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968214/range1968214/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968214/range1968214/1
type: Range
persistent identity: range1968214
display id: range1968214
version: 1
top level: ColE2
direction: direction/forward
start: 2401
end: 2544
orientation: inline

BBa_K131001
type: SequenceAnnotation
persistent identity: annotation1968210
display id: annotation1968210
version: 1
top level: ColE2
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968210/range1968210/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968210/range1968210/1
type: Range
persistent identity: range1968210
display id: range1968210
version: 1
top level: ColE2
direction: direction/forward
start: 1
end: 267
orientation: inline

BBa_K131004
type: SequenceAnnotation
persistent identity: annotation1968211
display id: annotation1968211
version: 1
top level: ColE2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968211/range1968211/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968211/range1968211/1
type: Range
persistent identity: range1968211
display id: range1968211
version: 1
top level: ColE2
direction: direction/forward
start: 331
end: 2076
orientation: inline

CeaB
type: SequenceAnnotation
persistent identity: annotation1968953
display id: annotation1968953
version: 1
top level: ColE2
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968953/range1968953/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968953/range1968953/1
type: Range
persistent identity: range1968953
display id: range1968953
version: 1
top level: ColE2
direction: direction/forward
start: 2074
end: 2076
orientation: inline

CeiB
type: SequenceAnnotation
persistent identity: annotation1968954
display id: annotation1968954
version: 1
top level: ColE2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968954/range1968954/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968954/range1968954/1
type: Range
persistent identity: range1968954
display id: range1968954
version: 1
top level: ColE2
direction: direction/forward
start: 2079
end: 2081
orientation: inline

CelB
type: SequenceAnnotation
persistent identity: annotation1968957
display id: annotation1968957
version: 1
top level: ColE2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968957/range1968957/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968957/range1968957/1
type: Range
persistent identity: range1968957
display id: range1968957
version: 1
top level: ColE2
direction: direction/forward
start: 2542
end: 2544
orientation: inline

BBa_K131007
type: SequenceAnnotation
persistent identity: annotation1968213
display id: annotation1968213
version: 1
top level: ColE2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968213/range1968213/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968213/range1968213/1
type: Range
persistent identity: range1968213
display id: range1968213
version: 1
top level: ColE2
direction: direction/forward
start: 2373
end: 2400
orientation: inline

CeiB
type: SequenceAnnotation
persistent identity: annotation1968955
display id: annotation1968955
version: 1
top level: ColE2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968955/range1968955/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968955/range1968955/1
type: Range
persistent identity: range1968955
display id: range1968955
version: 1
top level: ColE2
direction: direction/forward
start: 2337
end: 2339
orientation: inline

CelB
type: SequenceAnnotation
persistent identity: annotation1968956
display id: annotation1968956
version: 1
top level: ColE2
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968956/range1968956/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968956/range1968956/1
type: Range
persistent identity: range1968956
display id: range1968956
version: 1
top level: ColE2
direction: direction/forward
start: 2401
end: 2403
orientation: inline

CeaB
type: SequenceAnnotation
persistent identity: annotation1968952
display id: annotation1968952
version: 1
top level: ColE2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968952/range1968952/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968952/range1968952/1
type: Range
persistent identity: range1968952
display id: range1968952
version: 1
top level: ColE2
direction: direction/forward
start: 331
end: 333
orientation: inline

-35 Signal
type: SequenceAnnotation
persistent identity: annotation1968207
display id: annotation1968207
version: 1
top level: ColE2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131000/annotation1968207/range1968207/1

https://synbiohub.org/public/igem/BBa_K131000/annotation1968207/range1968207/1
type: Range
persistent identity: range1968207
display id: range1968207
version: 1
top level: ColE2
direction: direction/forward
start: 218
end: 224
orientation: inline

BBa_K515106
type: ComponentDefinition
persistent identity: BBa K515106
display id: BBa_K515106
version: 1
was generated by: iGEM to SBOL conversion
description: J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: genomic sequence
owned by: james
owned by: myers
mutable description: A composite part comprising of a J23100 promotor and PA2652 translational unit encoding extracellular chemoreceptor
discontinued: false
dominant: false
experience: experience/None
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Atipat Patharagulpong
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23103
component: BBa_E1010
component: BBa_K112806
component: BBa_K112805
component: BBa_B0034
sequence annotation: BBa_K112805
sequence annotation: BBa_J23103
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_K112806
sequence: https://synbiohub.org/public/igem/BBa_K515106_sequence/1

BBa_J23103
type: Component
persistent identity: component2258425
display id: component2258425
version: 1
top level: BBa_K515106
access: public
definition: BBa_J23103

BBa_E1010
type: Component
persistent identity: component2258430
display id: component2258430
version: 1
top level: BBa_K515106
access: public
definition: mRFP1

BBa_K112806
type: Component
persistent identity: component2258434
display id: component2258434
version: 1
top level: BBa_K515106
access: public
definition: BBa_K112806

BBa_K112805
type: Component
persistent identity: component2258432
display id: component2258432
version: 1
top level: BBa_K515106
access: public
definition: BBa_K112805

BBa_B0034
type: Component
persistent identity: component2258427
display id: component2258427
version: 1
top level: BBa_K515106
access: public
definition: BBa_B0034

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2258432
display id: annotation2258432
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258432/range2258432/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K515106/annotation2258432/range2258432/1
type: Range
persistent identity: range2258432
display id: range2258432
version: 1
top level: BBa_K515106
direction: direction/reverse
start: 776
end: 1451
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2258425
display id: annotation2258425
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258425/range2258425/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K515106/annotation2258425/range2258425/1
type: Range
persistent identity: range2258425
display id: range2258425
version: 1
top level: BBa_K515106
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2258427
display id: annotation2258427
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258427/range2258427/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K515106/annotation2258427/range2258427/1
type: Range
persistent identity: range2258427
display id: range2258427
version: 1
top level: BBa_K515106
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2258430
display id: annotation2258430
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258430/range2258430/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K515106/annotation2258430/range2258430/1
type: Range
persistent identity: range2258430
display id: range2258430
version: 1
top level: BBa_K515106
direction: direction/reverse
start: 62
end: 767
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2258434
display id: annotation2258434
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258434/range2258434/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K515106/annotation2258434/range2258434/1
type: Range
persistent identity: range2258434
display id: range2258434
version: 1
top level: BBa_K515106
direction: direction/reverse
start: 1460
end: 1973
orientation: inline

test
type: ComponentDefinition
persistent identity: BBa K1479015
display id: BBa_K1479015
version: 1
was generated by: iGEM to SBOL conversion
description: Just for test
creator: tester
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long
mutable notes: consideration consideration
mutable provenance: no source no source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: stop
sequence annotation: er
sequence annotation: dbn
sequence: https://synbiohub.org/public/igem/BBa_K1479015_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2399608
display id: annotation2399608
version: 1
top level: test
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479015/annotation2399608/range2399608/1

https://synbiohub.org/public/igem/BBa_K1479015/annotation2399608/range2399608/1
type: Range
persistent identity: range2399608
display id: range2399608
version: 1
direction: direction/forward
top level: test
start: 7
end: 8
orientation: inline

er
type: SequenceAnnotation
persistent identity: annotation2399606
display id: annotation2399606
version: 1
top level: test
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479015/annotation2399606/range2399606/1

https://synbiohub.org/public/igem/BBa_K1479015/annotation2399606/range2399606/1
type: Range
persistent identity: range2399606
display id: range2399606
version: 1
direction: direction/forward
top level: test
start: 1
end: 3
orientation: inline

dbn
type: SequenceAnnotation
persistent identity: annotation2399607
display id: annotation2399607
version: 1
top level: test
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1479015/annotation2399607/range2399607/1

https://synbiohub.org/public/igem/BBa_K1479015/annotation2399607/range2399607/1
type: Range
persistent identity: range2399607
display id: range2399607
version: 1
direction: direction/forward
top level: test
start: 4
end: 6
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1172912
type: ComponentDefinition
persistent identity: BBa K1172912
display id: BBa_K1172912
version: 1
was generated by: iGEM to SBOL conversion
description: Part 1 of the Biosystem TetOR alive (pRha TetR alr)
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
owned by: james
owned by: myers
mutable description: Part 1 of the Biosystem M (pRha TetR alr)
discontinued: false
dominant: false
experience: experience/None
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: K914004 Rhamnose promotor
sequence annotation: B0034
sequence annotation: K1172901 alanine racemase
sequence annotation: TetR C0040
sequence annotation: B0015 double terminator
sequence: https://synbiohub.org/public/igem/BBa_K1172912_sequence/1

K914004 Rhamnose promotor
type: SequenceAnnotation
persistent identity: annotation2371125
display id: annotation2371125
version: 1
top level: BBa_K1172912
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371125/range2371125/1

https://synbiohub.org/public/igem/BBa_K1172912/annotation2371125/range2371125/1
type: Range
persistent identity: range2371125
display id: range2371125
version: 1
top level: BBa_K1172912
direction: direction/forward
start: 1
end: 122
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2371126
display id: annotation2371126
version: 1
top level: BBa_K1172912
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371126/range2371126/1

https://synbiohub.org/public/igem/BBa_K1172912/annotation2371126/range2371126/1
type: Range
persistent identity: range2371126
display id: range2371126
version: 1
top level: BBa_K1172912
direction: direction/forward
start: 129
end: 140
orientation: inline

K1172901 alanine racemase
type: SequenceAnnotation
persistent identity: annotation2371129
display id: annotation2371129
version: 1
top level: BBa_K1172912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371129/range2371129/1

https://synbiohub.org/public/igem/BBa_K1172912/annotation2371129/range2371129/1
type: Range
persistent identity: range2371129
display id: range2371129
version: 1
top level: BBa_K1172912
direction: direction/forward
start: 826
end: 1905
orientation: inline

TetR C0040
type: SequenceAnnotation
persistent identity: annotation2371127
display id: annotation2371127
version: 1
top level: BBa_K1172912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371127/range2371127/1

https://synbiohub.org/public/igem/BBa_K1172912/annotation2371127/range2371127/1
type: Range
persistent identity: range2371127
display id: range2371127
version: 1
top level: BBa_K1172912
direction: direction/forward
start: 144
end: 803
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2371128
display id: annotation2371128
version: 1
top level: BBa_K1172912
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371128/range2371128/1

https://synbiohub.org/public/igem/BBa_K1172912/annotation2371128/range2371128/1
type: Range
persistent identity: range2371128
display id: range2371128
version: 1
top level: BBa_K1172912
direction: direction/forward
start: 811
end: 822
orientation: inline

B0015 double terminator
type: SequenceAnnotation
persistent identity: annotation2371130
display id: annotation2371130
version: 1
top level: BBa_K1172912
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371130/range2371130/1

https://synbiohub.org/public/igem/BBa_K1172912/annotation2371130/range2371130/1
type: Range
persistent identity: range2371130
display id: range2371130
version: 1
top level: BBa_K1172912
direction: direction/forward
start: 1914
end: 2042
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479009
display id: BBa_K1479009
version: 1
was generated by: iGEM to SBOL conversion
description: short short short
creator: designer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long long long long
mutable notes: notes notes notes notes
mutable provenance: source source source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: dist
sequence annotation: lalala
sequence: https://synbiohub.org/public/igem/BBa_K1479009_sequence/1

dist
type: SequenceAnnotation
persistent identity: annotation2399236
display id: annotation2399236
version: 1
top level: nickname
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1479009/annotation2399236/range2399236/1

https://synbiohub.org/public/igem/BBa_K1479009/annotation2399236/range2399236/1
type: Range
persistent identity: range2399236
display id: range2399236
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 2
orientation: inline

lalala
type: SequenceAnnotation
persistent identity: annotation2399238
display id: annotation2399238
version: 1
top level: nickname
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479009/annotation2399238/range2399238/1

https://synbiohub.org/public/igem/BBa_K1479009/annotation2399238/range2399238/1
type: Range
persistent identity: range2399238
display id: range2399238
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 3
orientation: inline

dist
type: SequenceAnnotation
persistent identity: annotation2399237
display id: annotation2399237
version: 1
top level: nickname
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479009/annotation2399237/range2399237/1

https://synbiohub.org/public/igem/BBa_K1479009/annotation2399237/range2399237/1
type: Range
persistent identity: range2399237
display id: range2399237
version: 1
direction: direction/reverse
top level: nickname
start: 2
end: 3
orientation: inline

BBa_K1223002
type: ComponentDefinition
persistent identity: BBa K1223002
display id: BBa_K1223002
version: 1
was generated by: iGEM to SBOL conversion
description: P.A.S.E. 2 cassette
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:43Z
mutable provenance: yeast
owned by: james
owned by: myers
mutable description: kanamicin ressistance gene (promoter+CDS)
discontinued: false
dominant: false
experience: experience/None
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: selection for cassette integration into E.coli BL21 genome
star: false
creator: Orr Schlesinger
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: KanR CDS
sequence annotation: start codon
sequence annotation: AraC\Lac promoter
sequence annotation: stop codon (TAG)
sequence annotation: homology region
sequence annotation: RBS
sequence annotation: Neo/Kan promoter
sequence annotation: KanR
sequence annotation: TyrS Homology region
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1223002_sequence/1

KanR CDS
type: SequenceAnnotation
persistent identity: annotation2337648
display id: annotation2337648
version: 1
top level: BBa_K1223002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337648/range2337648/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337648/range2337648/1
type: Range
persistent identity: range2337648
display id: range2337648
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 200
end: 994
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2337653
display id: annotation2337653
version: 1
top level: BBa_K1223002
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337653/range2337653/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337653/range2337653/1
type: Range
persistent identity: range2337653
display id: range2337653
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 62
end: 64
orientation: inline

AraC\Lac promoter
type: SequenceAnnotation
persistent identity: annotation2337645
display id: annotation2337645
version: 1
top level: BBa_K1223002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337645/range2337645/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337645/range2337645/1
type: Range
persistent identity: range2337645
display id: range2337645
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 87
end: 191
orientation: inline

stop codon (TAG)
type: SequenceAnnotation
persistent identity: annotation2337652
display id: annotation2337652
version: 1
top level: BBa_K1223002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337652/range2337652/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337652/range2337652/1
type: Range
persistent identity: range2337652
display id: range2337652
version: 1
top level: BBa_K1223002
direction: direction/forward
start: 47
end: 49
orientation: inline

homology region
type: SequenceAnnotation
persistent identity: annotation2337651
display id: annotation2337651
version: 1
top level: BBa_K1223002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337651/range2337651/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337651/range2337651/1
type: Range
persistent identity: range2337651
display id: range2337651
version: 1
top level: BBa_K1223002
direction: direction/forward
start: 1140
end: 1192
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2337646
display id: annotation2337646
version: 1
top level: BBa_K1223002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337646/range2337646/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337646/range2337646/1
type: Range
persistent identity: range2337646
display id: range2337646
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 71
end: 81
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2337655
display id: annotation2337655
version: 1
top level: BBa_K1223002
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337655/range2337655/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337655/range2337655/1
type: Range
persistent identity: range2337655
display id: range2337655
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 992
end: 994
orientation: inline

Neo/Kan promoter
type: SequenceAnnotation
persistent identity: annotation2337650
display id: annotation2337650
version: 1
top level: BBa_K1223002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337650/range2337650/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337650/range2337650/1
type: Range
persistent identity: range2337650
display id: range2337650
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 1083
end: 1132
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2337647
display id: annotation2337647
version: 1
top level: BBa_K1223002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337647/range2337647/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337647/range2337647/1
type: Range
persistent identity: range2337647
display id: range2337647
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 200
end: 1132
orientation: inline

TyrS Homology region
type: SequenceAnnotation
persistent identity: annotation2337644
display id: annotation2337644
version: 1
top level: BBa_K1223002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337644/range2337644/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337644/range2337644/1
type: Range
persistent identity: range2337644
display id: range2337644
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 5
end: 64
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2337654
display id: annotation2337654
version: 1
top level: BBa_K1223002
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337654/range2337654/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337654/range2337654/1
type: Range
persistent identity: range2337654
display id: range2337654
version: 1
top level: BBa_K1223002
direction: direction/reverse
start: 200
end: 202
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K150100
type: ComponentDefinition
persistent identity: BBa K150100
display id: BBa_K150100
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD00
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: original scar from BioBricks
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3314
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Christian Moritz
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150100_sequence/1

BBa_K150009
type: ComponentDefinition
persistent identity: BBa K150009
display id: BBa_K150009
version: 1
was generated by: iGEM to SBOL conversion
description: ColicinE1 Producer Controlled by 3OC6HSL Receiver Device
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE1 production. This activation of the lux pR promoter can additionally lead to expression of the kil protein by read through of the terminator t1 and therefore to lysis of the cell and colcin release. The colicinE1 immunity protein (imm) lying on the reverse strand is expressend under a constitutive promotor.
discontinued: false
dominant: false
experience: experience/Works
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K150007
component: BBa_K150008
component: BBa_R0062
component: BBa_K150100
component: BBa_R0040
component: BBa_K150104
component: BBa_C0062
component: BBa_B0034
component: BBa_K150107
component: BBa_B0012
component: BBa_B0010
component: BBa_K150005
sequence annotation: BBa_K150100
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_K150008
sequence annotation: BBa_K150107
sequence annotation: BBa_K150007
sequence annotation: BBa_K150005
sequence annotation: BBa_B0010
sequence annotation: BBa_K150104
sequence annotation: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K150009_sequence/1

BBa_K150007
type: Component
persistent identity: component2305761
display id: component2305761
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150007

BBa_K150008
type: Component
persistent identity: component2305757
display id: component2305757
version: 1
top level: BBa_K150009
access: public
definition: BamHI

BBa_R0062
type: Component
persistent identity: component2305749
display id: component2305749
version: 1
top level: BBa_K150009
access: public
definition: lux pR

BBa_K150100
type: Component
persistent identity: component2305739
display id: component2305739
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150100

BBa_R0040
type: Component
persistent identity: component2305724
display id: component2305724
version: 1
top level: BBa_K150009
access: public
definition: p(tetR)

BBa_K150100
type: Component
persistent identity: component2305729
display id: component2305729
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150100

BBa_K150104
type: Component
persistent identity: component2305754
display id: component2305754
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150104

BBa_C0062
type: Component
persistent identity: component2305735
display id: component2305735
version: 1
top level: BBa_K150009
access: public
definition: luxr

BBa_K150100
type: Component
persistent identity: component2305742
display id: component2305742
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150100

BBa_B0034
type: Component
persistent identity: component2305731
display id: component2305731
version: 1
top level: BBa_K150009
access: public
definition: BBa_B0034

BBa_K150107
type: Component
persistent identity: component2305732
display id: component2305732
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150107

BBa_B0012
type: Component
persistent identity: component2305743
display id: component2305743
version: 1
top level: BBa_K150009
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component2305740
display id: component2305740
version: 1
top level: BBa_K150009
access: public
definition: BBa_B0010

BBa_K150005
type: Component
persistent identity: component2305756
display id: component2305756
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150005

BBa_K150100
type: Component
persistent identity: component2305747
display id: component2305747
version: 1
top level: BBa_K150009
access: public
definition: BBa_K150100

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305742
display id: annotation2305742
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305742/range2305742/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305742/range2305742/1
type: Range
persistent identity: range2305742
display id: range2305742
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 950
end: 957
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2305749
display id: annotation2305749
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305749/range2305749/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K150009/annotation2305749/range2305749/1
type: Range
persistent identity: range2305749
display id: range2305749
version: 1
top level: BBa_K150009
direction: direction/forward
start: 1007
end: 1061
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2305743
display id: annotation2305743
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305743/range2305743/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K150009/annotation2305743/range2305743/1
type: Range
persistent identity: range2305743
display id: range2305743
version: 1
top level: BBa_K150009
direction: direction/forward
start: 958
end: 998
orientation: inline

BBa_K150008
type: SequenceAnnotation
persistent identity: annotation2305757
display id: annotation2305757
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305757/range2305757/1
component: BBa_K150008

https://synbiohub.org/public/igem/BBa_K150009/annotation2305757/range2305757/1
type: Range
persistent identity: range2305757
display id: range2305757
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 1083
end: 1088
orientation: inline

BBa_K150107
type: SequenceAnnotation
persistent identity: annotation2305732
display id: annotation2305732
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305732/range2305732/1
component: BBa_K150107

https://synbiohub.org/public/igem/BBa_K150009/annotation2305732/range2305732/1
type: Range
persistent identity: range2305732
display id: range2305732
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 75
end: 80
orientation: inline

BBa_K150007
type: SequenceAnnotation
persistent identity: annotation2305761
display id: annotation2305761
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305761/range2305761/1
component: BBa_K150007

https://synbiohub.org/public/igem/BBa_K150009/annotation2305761/range2305761/1
type: Range
persistent identity: range2305761
display id: range2305761
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 1089
end: 3180
orientation: inline

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation2305756
display id: annotation2305756
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305756/range2305756/1
component: BBa_K150005

https://synbiohub.org/public/igem/BBa_K150009/annotation2305756/range2305756/1
type: Range
persistent identity: range2305756
display id: range2305756
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 1070
end: 1082
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2305740
display id: annotation2305740
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305740/range2305740/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K150009/annotation2305740/range2305740/1
type: Range
persistent identity: range2305740
display id: range2305740
version: 1
top level: BBa_K150009
direction: direction/forward
start: 870
end: 949
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305739
display id: annotation2305739
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305739/range2305739/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305739/range2305739/1
type: Range
persistent identity: range2305739
display id: range2305739
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 862
end: 869
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305729
display id: annotation2305729
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305729/range2305729/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305729/range2305729/1
type: Range
persistent identity: range2305729
display id: range2305729
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 55
end: 62
orientation: inline

BBa_K150104
type: SequenceAnnotation
persistent identity: annotation2305754
display id: annotation2305754
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305754/range2305754/1
component: BBa_K150104

https://synbiohub.org/public/igem/BBa_K150009/annotation2305754/range2305754/1
type: Range
persistent identity: range2305754
display id: range2305754
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 1062
end: 1069
orientation: inline

BBa_K150100
type: SequenceAnnotation
persistent identity: annotation2305747
display id: annotation2305747
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305747/range2305747/1
component: BBa_K150100

https://synbiohub.org/public/igem/BBa_K150009/annotation2305747/range2305747/1
type: Range
persistent identity: range2305747
display id: range2305747
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 999
end: 1006
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2305735
display id: annotation2305735
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305735/range2305735/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K150009/annotation2305735/range2305735/1
type: Range
persistent identity: range2305735
display id: range2305735
version: 1
top level: BBa_K150009
direction: direction/forward
start: 81
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2305724
display id: annotation2305724
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305724/range2305724/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K150009/annotation2305724/range2305724/1
type: Range
persistent identity: range2305724
display id: range2305724
version: 1
top level: BBa_K150009
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2305731
display id: annotation2305731
version: 1
top level: BBa_K150009
location: https://synbiohub.org/public/igem/BBa_K150009/annotation2305731/range2305731/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K150009/annotation2305731/range2305731/1
type: Range
persistent identity: range2305731
display id: range2305731
version: 1
top level: BBa_K150009
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K1639022
type: ComponentDefinition
persistent identity: BBa K1639022
display id: BBa_K1639022
version: 1
was generated by: iGEM to SBOL conversion
description: pCons-TetR-pTet-TevProtease Suicide Switch
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T10:18:35Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: This is a kill switch part of our engineering E. Coli. pTet is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. In the presence of aTc TetR repression inhibited and Tev Protease produce by Ptet promoter. Tev Protease release Pexiganan from DAMP and E. Coli kill switch works succesfully.
discontinued: false
dominant: false
experience: experience/None
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Mustafa Yılmaz
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_J23100
component: BBa_C0040
component: BBa_K1639008
sequence annotation: BBa_K1639008
sequence annotation: BBa_C0040
sequence annotation: BBa_J23100
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1639022_sequence/1

BBa_R0040
type: Component
persistent identity: component2472056
display id: component2472056
version: 1
top level: BBa_K1639022
access: public
definition: p(tetR)

BBa_J23100
type: Component
persistent identity: component2472051
display id: component2472051
version: 1
top level: BBa_K1639022
access: public
definition: BBa_J23100

BBa_C0040
type: Component
persistent identity: component2472055
display id: component2472055
version: 1
top level: BBa_K1639022
access: public
definition: tetR

BBa_K1639008
type: Component
persistent identity: component2472065
display id: component2472065
version: 1
top level: BBa_K1639022
access: public
definition: BBa_K1639008

BBa_K1639008
type: SequenceAnnotation
persistent identity: annotation2472065
display id: annotation2472065
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472065/range2472065/1
component: BBa_K1639008

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472065/range2472065/1
type: Range
persistent identity: range2472065
display id: range2472065
version: 1
top level: BBa_K1639022
direction: direction/reverse
start: 797
end: 1546
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2472055
display id: annotation2472055
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472055/range2472055/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472055/range2472055/1
type: Range
persistent identity: range2472055
display id: range2472055
version: 1
top level: BBa_K1639022
direction: direction/reverse
start: 42
end: 726
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2472051
display id: annotation2472051
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472051/range2472051/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472051/range2472051/1
type: Range
persistent identity: range2472051
display id: range2472051
version: 1
top level: BBa_K1639022
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2472056
display id: annotation2472056
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472056/range2472056/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472056/range2472056/1
type: Range
persistent identity: range2472056
display id: range2472056
version: 1
top level: BBa_K1639022
direction: direction/forward
start: 735
end: 788
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479007
display id: BBa_K1479007
version: 1
was generated by: iGEM to SBOL conversion
description: Just for upload test
creator: ChenYB
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: dist
sequence annotation: lalala
sequence: https://synbiohub.org/public/igem/BBa_K1479007_sequence/1

dist
type: SequenceAnnotation
persistent identity: annotation2398379
display id: annotation2398379
version: 1
top level: nickname
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479007/annotation2398379/range2398379/1

https://synbiohub.org/public/igem/BBa_K1479007/annotation2398379/range2398379/1
type: Range
persistent identity: range2398379
display id: range2398379
version: 1
direction: direction/reverse
top level: nickname
start: 2
end: 3
orientation: inline

lalala
type: SequenceAnnotation
persistent identity: annotation2398380
display id: annotation2398380
version: 1
top level: nickname
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479007/annotation2398380/range2398380/1

https://synbiohub.org/public/igem/BBa_K1479007/annotation2398380/range2398380/1
type: Range
persistent identity: range2398380
display id: range2398380
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 3
orientation: inline

dist
type: SequenceAnnotation
persistent identity: annotation2398378
display id: annotation2398378
version: 1
top level: nickname
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1479007/annotation2398378/range2398378/1

https://synbiohub.org/public/igem/BBa_K1479007/annotation2398378/range2398378/1
type: Range
persistent identity: range2398378
display id: range2398378
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 2
orientation: inline

BBa_K1479006
type: ComponentDefinition
persistent identity: BBa K1479006
display id: BBa_K1479006
version: 1
was generated by: iGEM to SBOL conversion
description: Just for upload test
creator: Weijian Cao
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: dist
sequence annotation: lalala
sequence: https://synbiohub.org/public/igem/BBa_K1479006_sequence/1

dist
type: SequenceAnnotation
persistent identity: annotation2398348
display id: annotation2398348
version: 1
top level: BBa_K1479006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479006/annotation2398348/range2398348/1

https://synbiohub.org/public/igem/BBa_K1479006/annotation2398348/range2398348/1
type: Range
persistent identity: range2398348
display id: range2398348
version: 1
direction: direction/reverse
top level: BBa_K1479006
start: 2
end: 3
orientation: inline

dist
type: SequenceAnnotation
persistent identity: annotation2398347
display id: annotation2398347
version: 1
top level: BBa_K1479006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1479006/annotation2398347/range2398347/1

https://synbiohub.org/public/igem/BBa_K1479006/annotation2398347/range2398347/1
type: Range
persistent identity: range2398347
display id: range2398347
version: 1
direction: direction/forward
top level: BBa_K1479006
start: 1
end: 2
orientation: inline

lalala
type: SequenceAnnotation
persistent identity: annotation2398349
display id: annotation2398349
version: 1
top level: BBa_K1479006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479006/annotation2398349/range2398349/1

https://synbiohub.org/public/igem/BBa_K1479006/annotation2398349/range2398349/1
type: Range
persistent identity: range2398349
display id: range2398349
version: 1
direction: direction/forward
top level: BBa_K1479006
start: 1
end: 3
orientation: inline

BBa_K914012
type: ComponentDefinition
persistent identity: BBa K914012
display id: BBa_K914012
version: 1
was generated by: iGEM to SBOL conversion
description: pSB1A2 with 1 amber mutation
created: 2012-10-04T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: This gene is mutated by the PCR quick change mutagenesis.
owned by: james
owned by: myers
mutable description: pSB1A2 have a double mutation in the 80th serine (TCC->TAG) of the APr gene. This plasmid need a tRNA amber suppressor to express this gene. So in case of horizontal gene transfer this gene won't be expressed and then the plasmid will be lost more easily.
discontinued: false
dominant: false
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 2 oligo mutated on 2 positions were used for this purpose.
star: false
creator: Jean Cury
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K914012_sequence/1

BBa_K914011
type: ComponentDefinition
persistent identity: BBa K914011
display id: BBa_K914011
version: 1
was generated by: iGEM to SBOL conversion
description: supD-T - intermediate
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: made from 2 biobricks.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the intermediate of the K914000 part, so you can change the promoter easily.
discontinued: false
dominant: false
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The terminator is mutated, but it does not impact the function.
star: false
creator: Jean Cury
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: modified thr terminator
sequence annotation: B1006
sequence annotation: BBa_K228001
sequence: https://synbiohub.org/public/igem/BBa_K914011_sequence/1

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2189725
display id: annotation2189725
version: 1
top level: BBa_K914011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K914011/annotation2189725/range2189725/1

https://synbiohub.org/public/igem/BBa_K914011/annotation2189725/range2189725/1
type: Range
persistent identity: range2189725
display id: range2189725
version: 1
top level: BBa_K914011
direction: direction/forward
start: 154
end: 175
orientation: inline

B1006
type: SequenceAnnotation
persistent identity: annotation2189724
display id: annotation2189724
version: 1
top level: BBa_K914011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914011/annotation2189724/range2189724/1

https://synbiohub.org/public/igem/BBa_K914011/annotation2189724/range2189724/1
type: Range
persistent identity: range2189724
display id: range2189724
version: 1
top level: BBa_K914011
direction: direction/forward
start: 145
end: 183
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2189723
display id: annotation2189723
version: 1
top level: BBa_K914011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914011/annotation2189723/range2189723/1

https://synbiohub.org/public/igem/BBa_K914011/annotation2189723/range2189723/1
type: Range
persistent identity: range2189723
display id: range2189723
version: 1
top level: BBa_K914011
direction: direction/reverse
start: 1
end: 136
orientation: inline

BBa_K1172911
type: ComponentDefinition
persistent identity: BBa K1172911
display id: BBa_K1172911
version: 1
was generated by: iGEM to SBOL conversion
description: Biosafety-System Lac of Growth (lacI)
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
owned by: james
owned by: myers
mutable description: Biosafety-System L (lacI)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172908
component: BBa_K741002
sequence annotation: BBa_K1172908
sequence annotation: BBa_K741002
sequence: https://synbiohub.org/public/igem/BBa_K1172911_sequence/1

BBa_K1172908
type: Component
persistent identity: component2359493
display id: component2359493
version: 1
top level: BBa_K1172911
access: public
definition: BBa_K1172908

BBa_K741002
type: Component
persistent identity: component2359511
display id: component2359511
version: 1
top level: BBa_K1172911
access: public
definition: BBa_K741002

BBa_K1172908
type: SequenceAnnotation
persistent identity: annotation2359493
display id: annotation2359493
version: 1
top level: BBa_K1172911
location: https://synbiohub.org/public/igem/BBa_K1172911/annotation2359493/range2359493/1
component: BBa_K1172908

https://synbiohub.org/public/igem/BBa_K1172911/annotation2359493/range2359493/1
type: Range
persistent identity: range2359493
display id: range2359493
version: 1
top level: BBa_K1172911
direction: direction/reverse
start: 1
end: 2509
orientation: inline

BBa_K741002
type: SequenceAnnotation
persistent identity: annotation2359511
display id: annotation2359511
version: 1
top level: BBa_K1172911
location: https://synbiohub.org/public/igem/BBa_K1172911/annotation2359511/range2359511/1
component: BBa_K741002

https://synbiohub.org/public/igem/BBa_K1172911/annotation2359511/range2359511/1
type: Range
persistent identity: range2359511
display id: range2359511
version: 1
top level: BBa_K1172911
direction: direction/reverse
start: 2518
end: 3600
orientation: inline

BBa_K628000
type: ComponentDefinition
persistent identity: BBa K628000
display id: BBa_K628000
version: 1
was generated by: iGEM to SBOL conversion
description: codes for protegrin-1 (anti-microbial peptide)
created: 2011-09-18T11:00:00Z
modified: 2016-02-01T12:54:06Z
mutable provenance: This sequence was derived from protegrin-1 found in porcine leukocytes. The website where we found the sequence is:
owned by: james
owned by: myers
mutable description: This is the coding region for protegrin-1.
discontinued: false
dominant: false
group u list: _800_
m user id: 4206
owner id: 9738
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Charles Thompson
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K628000_sequence/1

toxin ParE
type: ComponentDefinition
persistent identity: BBa K2150104
display id: BBa_K2150104
version: 1
was generated by: iGEM to SBOL conversion
description: 6249 toxin CDS
created: 2016-10-11T11:00:00Z
modified: 2016-10-13T11:15:01Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Zepeng Mu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ParD2
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K2150104_sequence/1

ParD2
type: SequenceAnnotation
persistent identity: annotation2499113
display id: annotation2499113
version: 1
top level: toxin ParE
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2150104/annotation2499113/range2499113/1

https://synbiohub.org/public/igem/BBa_K2150104/annotation2499113/range2499113/1
type: Range
persistent identity: range2499113
display id: range2499113
version: 1
top level: toxin ParE
direction: direction/forward
start: 1
end: 316
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2499115
display id: annotation2499115
version: 1
top level: toxin ParE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2150104/annotation2499115/range2499115/1

https://synbiohub.org/public/igem/BBa_K2150104/annotation2499115/range2499115/1
type: Range
persistent identity: range2499115
display id: range2499115
version: 1
top level: toxin ParE
direction: direction/forward
start: 317
end: 319
orientation: inline

BBa_K1720002
type: ComponentDefinition
persistent identity: BBa K1720002
display id: BBa_K1720002
version: 1
was generated by: iGEM to SBOL conversion
description: Hypoxia-induced promotor
creator: Yuanbin Cui, Yaran Zhao
created: 2015-08-24T11:00:00Z
modified: 2015-09-18T07:26:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2140_
m user id: 25248
owner id: 25248
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a part used for coding soluble human guanylate cyclase subunit:Alpha 3.Alpha 3 unit interacts with a beta subunit to form the guanylate cyclase enzyme. Soluble guanylate cyclases(sGC) are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. It is important for smooth muscle relaxation in the cardiovascular system. sGC contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs.There are several different isoforms of sGC subunit ,but the cardiovascular system abundant with alpha 3 unit and beta 3 unit. This part with a GFP reporter was then transfected into HEK293 cells by lentiviral Vector and in vivo green fluorescence signal was observed under fluorescence microscope, it meant that this part was transfected into HEK293 cells successfully.Then we used qRT-PCR to observe transcriptional level of alpha 3 unit.Finally we detected the cGMP concentration with Elisa Kit. And the results are as follow:
mutable notes: This part do not have promoter because the promoter we used in our project is EF1A which consists the restriction sites.So before using this part please add a promoter.
mutable provenance: We find the information of Human guanylate cyclase??1,soluble, alpha 3 unit here：http://www.ncbi.nlm.nih.gov/gene/2982
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1720002_sequence/1

BBa_K772101
type: ComponentDefinition
persistent identity: BBa K772101
display id: BBa_K772101
version: 1
was generated by: iGEM to SBOL conversion
description: Self-Destruction Kit: Endolysin + Holin
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Endolysin is a lysozyme which cleaves the glycosidic bond between the C-1 of N-acetylmuramic acid (NAM) and the C-4 of N-acetylglucosamine (NAG) in the bacterial peptidoglycan (PG). This leads to the complete lysis of the cell. Holin is a protein that assembles together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. It is sourced from T4 bacteriophage. This composite part can be used to induce self-destruction with a regulatory device in the upstream region.
mutable notes: There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of.
mutable provenance: Gene sequences of endolysin and holin are gathered from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112806
component: BBa_K124014
sequence annotation: BBa_K124014
sequence annotation: BBa_K112806
sequence: https://synbiohub.org/public/igem/BBa_K772101_sequence/1

BBa_K112806
type: Component
persistent identity: component2202511
display id: component2202511
version: 1
top level: BBa_K772101
access: public
definition: BBa_K112806

BBa_K124014
type: Component
persistent identity: component2202512
display id: component2202512
version: 1
top level: BBa_K772101
access: public
definition: https://synbiohub.org/public/igem/BBa_K124014/1

BBa_K124014
type: SequenceAnnotation
persistent identity: annotation2202512
display id: annotation2202512
version: 1
top level: BBa_K772101
location: https://synbiohub.org/public/igem/BBa_K772101/annotation2202512/range2202512/1
component: BBa_K124014

https://synbiohub.org/public/igem/BBa_K772101/annotation2202512/range2202512/1
type: Range
persistent identity: range2202512
display id: range2202512
version: 1
direction: direction/reverse
top level: BBa_K772101
start: 523
end: 839
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2202511
display id: annotation2202511
version: 1
top level: BBa_K772101
location: https://synbiohub.org/public/igem/BBa_K772101/annotation2202511/range2202511/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K772101/annotation2202511/range2202511/1
type: Range
persistent identity: range2202511
display id: range2202511
version: 1
direction: direction/reverse
top level: BBa_K772101
start: 1
end: 514
orientation: inline

BBa_K914018
type: ComponentDefinition
persistent identity: BBa K914018
display id: BBa_K914018
version: 1
was generated by: iGEM to SBOL conversion
description: P1003** Kan resistant gene with 2 Amber Codon
created: 2012-10-24T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: This part is made from the BBa_K914009 part from which I add a second mutation with the QuickChange Site-directed mutagenesis kit, Agilent.
owned by: james
owned by: myers
mutable description: The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: I have need to design the primers that will introduce a mutation from a serine that was closer to the TAG codon (either 1, 2 or 3 mutations, here it was 1 and 2 mutations for both mutations), and it is necessary to have the amber codon in the middle of the coding sequence, not in the beginning, to be not interpreted as a alternative start codon for instance.
star: false
creator: Jean Cury
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_K914009
sequence annotation: BBa_P1003
sequence annotation: Ser133->Amber codon
sequence annotation: Ser203->Amber mutation
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_K914018_sequence/1

BBa_K914009
type: SequenceAnnotation
persistent identity: annotation2212597
display id: annotation2212597
version: 1
top level: BBa_K914018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212597/range2212597/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212597/range2212597/1
type: Range
persistent identity: range2212597
display id: range2212597
version: 1
top level: BBa_K914018
direction: direction/reverse
start: 1
end: 967
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2212594
display id: annotation2212594
version: 1
top level: BBa_K914018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212594/range2212594/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212594/range2212594/1
type: Range
persistent identity: range2212594
display id: range2212594
version: 1
top level: BBa_K914018
direction: direction/forward
start: 1
end: 967
orientation: inline

Ser133->Amber codon
type: SequenceAnnotation
persistent identity: annotation2212596
display id: annotation2212596
version: 1
top level: BBa_K914018
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212596/range2212596/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212596/range2212596/1
type: Range
persistent identity: range2212596
display id: range2212596
version: 1
top level: BBa_K914018
direction: direction/forward
start: 546
end: 546
orientation: inline

Ser203->Amber mutation
type: SequenceAnnotation
persistent identity: annotation2212599
display id: annotation2212599
version: 1
top level: BBa_K914018
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212599/range2212599/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212599/range2212599/1
type: Range
persistent identity: range2212599
display id: range2212599
version: 1
top level: BBa_K914018
direction: direction/forward
start: 609
end: 610
orientation: inline

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2212595
display id: annotation2212595
version: 1
top level: BBa_K914018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914018/annotation2212595/range2212595/1

https://synbiohub.org/public/igem/BBa_K914018/annotation2212595/range2212595/1
type: Range
persistent identity: range2212595
display id: range2212595
version: 1
top level: BBa_K914018
direction: direction/forward
start: 149
end: 967
orientation: inline

BamHI
type: ComponentDefinition
persistent identity: BBa K150008
display id: BBa_K150008
version: 1
was generated by: iGEM to SBOL conversion
description: BamHI restriction enzyme site
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: BamHI restriction site
discontinued: false
dominant: true
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K150008_sequence/1

BBa_K1172902
type: ComponentDefinition
persistent identity: BBa K1172902
display id: BBa_K1172902
version: 1
was generated by: iGEM to SBOL conversion
description: alanine racemase (''alr'') under the control of the P''tac'' promoter
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
mutable provenance: Genom Escherichia coli
owned by: james
owned by: myers
mutable description: Alanin_Racemase (alr) under the control of the ptac-Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Complementation of D-Alanine-Auxotrophy
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: ptac-promoter
sequence annotation: K1172901 (Alanine-Racemase)
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1172902_sequence/1

ptac-promoter
type: SequenceAnnotation
persistent identity: annotation2355534
display id: annotation2355534
version: 1
top level: BBa_K1172902
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172902/annotation2355534/range2355534/1

https://synbiohub.org/public/igem/BBa_K1172902/annotation2355534/range2355534/1
type: Range
persistent identity: range2355534
display id: range2355534
version: 1
top level: BBa_K1172902
direction: direction/forward
start: 1
end: 28
orientation: inline

K1172901 (Alanine-Racemase)
type: SequenceAnnotation
persistent identity: annotation2357202
display id: annotation2357202
version: 1
top level: BBa_K1172902
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172902/annotation2357202/range2357202/1

https://synbiohub.org/public/igem/BBa_K1172902/annotation2357202/range2357202/1
type: Range
persistent identity: range2357202
display id: range2357202
version: 1
top level: BBa_K1172902
direction: direction/forward
start: 55
end: 1134
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2357201
display id: annotation2357201
version: 1
top level: BBa_K1172902
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172902/annotation2357201/range2357201/1

https://synbiohub.org/public/igem/BBa_K1172902/annotation2357201/range2357201/1
type: Range
persistent identity: range2357201
display id: range2357201
version: 1
top level: BBa_K1172902
direction: direction/forward
start: 37
end: 48
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479012
display id: BBa_K1479012
version: 1
was generated by: iGEM to SBOL conversion
description: short short short
creator: designer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long long long long
mutable notes: notes notes notes notes
mutable provenance: source source source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence: https://synbiohub.org/public/igem/BBa_K1479012_sequence/1

BBa_K879918
type: ComponentDefinition
persistent identity: BBa K879918
display id: BBa_K879918
version: 1
was generated by: iGEM to SBOL conversion
description: TetO ori
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: TetO ori
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: .
star: false
creator: Nien-Tsu Tseng
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: TetO operator
sequence annotation: T0 terminator
sequence annotation: -35
sequence annotation: pMB1 derived replication origin
sequence annotation: RNA II
sequence annotation: VR
sequence annotation: Chloramphenicol resistance
sequence annotation: VF2
sequence annotation: BioBrick suffix
sequence annotation: BioBrick prefix
sequence annotation: -10
sequence annotation: E coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K879918_sequence/1

TetO operator
type: SequenceAnnotation
persistent identity: annotation2210755
display id: annotation2210755
version: 1
top level: BBa_K879918
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1
type: Range
persistent identity: range2210755
display id: range2210755
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 842
end: 858
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210757
display id: annotation2210757
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1
type: Range
persistent identity: range2210757
display id: range2210757
version: 1
top level: BBa_K879918
direction: direction/forward
start: 1044
end: 1149
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2210756
display id: annotation2210756
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1
type: Range
persistent identity: range2210756
display id: range2210756
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 859
end: 864
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210752
display id: annotation2210752
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1
type: Range
persistent identity: range2210752
display id: range2210752
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 263
end: 877
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210753
display id: annotation2210753
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1
type: Range
persistent identity: range2210753
display id: range2210753
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 277
end: 826
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2210751
display id: annotation2210751
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1
type: Range
persistent identity: range2210751
display id: range2210751
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 159
end: 178
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210758
display id: annotation2210758
version: 1
top level: BBa_K879918
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1
type: Range
persistent identity: range2210758
display id: range2210758
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 1162
end: 1821
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2210759
display id: annotation2210759
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1
type: Range
persistent identity: range2210759
display id: range2210759
version: 1
top level: BBa_K879918
direction: direction/forward
start: 1935
end: 1954
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210749
display id: annotation2210749
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1
type: Range
persistent identity: range2210749
display id: range2210749
version: 1
top level: BBa_K879918
direction: direction/forward
start: 1
end: 21
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210775
display id: annotation2210775
version: 1
top level: BBa_K879918
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1
type: Range
persistent identity: range2210775
display id: range2210775
version: 1
top level: BBa_K879918
direction: direction/forward
start: 2053
end: 2072
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210754
display id: annotation2210754
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1
type: Range
persistent identity: range2210754
display id: range2210754
version: 1
top level: BBa_K879918
direction: direction/reverse
start: 836
end: 841
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210750
display id: annotation2210750
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1
type: Range
persistent identity: range2210750
display id: range2210750
version: 1
top level: BBa_K879918
direction: direction/forward
start: 22
end: 95
orientation: inline

testtest
type: ComponentDefinition
persistent identity: BBa K1479018
display id: BBa_K1479018
version: 1
was generated by: iGEM to SBOL conversion
description: Just for test
creator: tester
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: fsdfasfadsfasdfadsfaf
mutable notes: sdfasdfsdfasdfsfasdfasdfasdfasd
mutable provenance: dsfadsfadsfdasfasdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain

nickname
type: ComponentDefinition
persistent identity: BBa K1479008
display id: BBa_K1479008
version: 1
was generated by: iGEM to SBOL conversion
description: short short short
creator: designer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long long long long
mutable notes: notes notes notes notes
mutable provenance: source source source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: label2
sequence annotation: label3
sequence annotation: label1
sequence: https://synbiohub.org/public/igem/BBa_K1479008_sequence/1

label2
type: SequenceAnnotation
persistent identity: annotation2417083
display id: annotation2417083
version: 1
top level: nickname
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479008/annotation2417083/range2417083/1

https://synbiohub.org/public/igem/BBa_K1479008/annotation2417083/range2417083/1
type: Range
persistent identity: range2417083
display id: range2417083
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 2
orientation: inline

label3
type: SequenceAnnotation
persistent identity: annotation2417084
display id: annotation2417084
version: 1
top level: nickname
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479008/annotation2417084/range2417084/1

https://synbiohub.org/public/igem/BBa_K1479008/annotation2417084/range2417084/1
type: Range
persistent identity: range2417084
display id: range2417084
version: 1
direction: direction/reverse
top level: nickname
start: 3
end: 4
orientation: inline

label1
type: SequenceAnnotation
persistent identity: annotation2417082
display id: annotation2417082
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479008/annotation2417082/range2417082/1

https://synbiohub.org/public/igem/BBa_K1479008/annotation2417082/range2417082/1
type: Range
persistent identity: range2417082
display id: range2417082
version: 1
direction: direction/forward
top level: nickname
start: 2
end: 3
orientation: inline

BBa_K1172908
type: ComponentDefinition
persistent identity: BBa K1172908
display id: BBa_K1172908
version: 1
was generated by: iGEM to SBOL conversion
description: Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr)
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
owned by: james
owned by: myers
mutable description: Part 1 of the Biosafety-System L (pRha lacI alr)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0034
sequence annotation: lacI-LVA (C0012)
sequence annotation: pRha (K914004)
sequence annotation: double Terminator (B0015)
sequence annotation: Alanine-Racemase (K1172901)
sequence: https://synbiohub.org/public/igem/BBa_K1172908_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2359484
display id: annotation2359484
version: 1
top level: BBa_K1172908
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359484/range2359484/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359484/range2359484/1
type: Range
persistent identity: range2359484
display id: range2359484
version: 1
top level: BBa_K1172908
direction: direction/forward
start: 1278
end: 1289
orientation: inline

lacI-LVA (C0012)
type: SequenceAnnotation
persistent identity: annotation2359483
display id: annotation2359483
version: 1
top level: BBa_K1172908
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359483/range2359483/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359483/range2359483/1
type: Range
persistent identity: range2359483
display id: range2359483
version: 1
top level: BBa_K1172908
direction: direction/forward
start: 144
end: 1271
orientation: inline

pRha (K914004)
type: SequenceAnnotation
persistent identity: annotation2355542
display id: annotation2355542
version: 1
top level: BBa_K1172908
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2355542/range2355542/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2355542/range2355542/1
type: Range
persistent identity: range2355542
display id: range2355542
version: 1
top level: BBa_K1172908
direction: direction/forward
start: 1
end: 122
orientation: inline

double Terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2359486
display id: annotation2359486
version: 1
top level: BBa_K1172908
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359486/range2359486/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359486/range2359486/1
type: Range
persistent identity: range2359486
display id: range2359486
version: 1
top level: BBa_K1172908
direction: direction/forward
start: 2381
end: 2509
orientation: inline

Alanine-Racemase (K1172901)
type: SequenceAnnotation
persistent identity: annotation2359485
display id: annotation2359485
version: 1
top level: BBa_K1172908
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359485/range2359485/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359485/range2359485/1
type: Range
persistent identity: range2359485
display id: range2359485
version: 1
top level: BBa_K1172908
direction: direction/forward
start: 1293
end: 2372
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2359482
display id: annotation2359482
version: 1
top level: BBa_K1172908
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359482/range2359482/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359482/range2359482/1
type: Range
persistent identity: range2359482
display id: range2359482
version: 1
top level: BBa_K1172908
direction: direction/forward
start: 129
end: 140
orientation: inline

nick
type: ComponentDefinition
persistent identity: BBa K1479014
display id: BBa_K1479014
version: 1
was generated by: iGEM to SBOL conversion
description: Just for Test
creator: designer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long description long long long just for test
mutable notes: design consideration
mutable provenance: no source of this part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence annotation: start
sequence annotation: end
sequence: https://synbiohub.org/public/igem/BBa_K1479014_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2399596
display id: annotation2399596
version: 1
top level: nick
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1479014/annotation2399596/range2399596/1

https://synbiohub.org/public/igem/BBa_K1479014/annotation2399596/range2399596/1
type: Range
persistent identity: range2399596
display id: range2399596
version: 1
direction: direction/forward
top level: nick
start: 1
end: 3
orientation: inline

end
type: SequenceAnnotation
persistent identity: annotation2399597
display id: annotation2399597
version: 1
top level: nick
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479014/annotation2399597/range2399597/1

https://synbiohub.org/public/igem/BBa_K1479014/annotation2399597/range2399597/1
type: Range
persistent identity: range2399597
display id: range2399597
version: 1
direction: direction/forward
top level: nick
start: 5
end: 6
orientation: inline

T7RSR
type: ComponentDefinition
persistent identity: BBa K786031
display id: BBa_K786031
version: 1
was generated by: iGEM to SBOL conversion
description: T7promoter-RSR-T7teminator
creator: Kaiyin Feng
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1041_
m user id: 0
owner id: 14280
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system.
mutable notes: The repeat-spacer-repeat region design should be consistent with the operating principles of E.coli's CRISPR/cas system.
mutable provenance: The 'repeat' sequence in CRISPR/cas system of E.coli strain K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7-terminator
sequence annotation: T7-promoter
sequence annotation: repeat-spacer-repeat region
sequence: https://synbiohub.org/public/igem/BBa_K786031_sequence/1

T7-terminator
type: SequenceAnnotation
persistent identity: annotation2203443
display id: annotation2203443
version: 1
top level: T7RSR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203443/range2203443/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203443/range2203443/1
type: Range
persistent identity: range2203443
display id: range2203443
version: 1
direction: direction/forward
top level: T7RSR
start: 812
end: 956
orientation: inline

T7-promoter
type: SequenceAnnotation
persistent identity: annotation2203441
display id: annotation2203441
version: 1
top level: T7RSR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203441/range2203441/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203441/range2203441/1
type: Range
persistent identity: range2203441
display id: range2203441
version: 1
direction: direction/forward
top level: T7RSR
start: 1
end: 23
orientation: inline

repeat-spacer-repeat region
type: SequenceAnnotation
persistent identity: annotation2203442
display id: annotation2203442
version: 1
top level: T7RSR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203442/range2203442/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203442/range2203442/1
type: Range
persistent identity: range2203442
display id: range2203442
version: 1
direction: direction/forward
top level: T7RSR
start: 31
end: 803
orientation: inline

BBa_K150104
type: ComponentDefinition
persistent identity: BBa K150104
display id: BBa_K150104
version: 1
was generated by: iGEM to SBOL conversion
description: linkerHD04
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:47Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: DNA linker region between lux pR promoter BBa_R0062 and ribosome binding site pTrc99a (BBa_) for the ColicinE1 Producer Controlled by 3OC6HSL Receiver Device (BBa_).
discontinued: false
dominant: false
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Pascal Kraemer
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: linkerHD04
sequence: https://synbiohub.org/public/igem/BBa_K150104_sequence/1

linkerHD04
type: SequenceAnnotation
persistent identity: annotation1983191
display id: annotation1983191
version: 1
top level: BBa_K150104
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K150104/annotation1983191/range1983191/1

https://synbiohub.org/public/igem/BBa_K150104/annotation1983191/range1983191/1
type: Range
persistent identity: range1983191
display id: range1983191
version: 1
top level: BBa_K150104
direction: direction/forward
start: 1
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CAP binding site
sequence annotation: LacI binding site
sequence annotation: -35
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: start
sequence annotation: end of LacI coding region (inactive)
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_K1172901
type: ComponentDefinition
persistent identity: BBa K1172901
display id: BBa_K1172901
version: 1
was generated by: iGEM to SBOL conversion
description: Alanine racemase from ''E. coli''
created: 2013-08-25T11:00:00Z
modified: 2015-05-08T01:09:35Z
mutable provenance: E. coli KRX genomic DNA
owned by: myers
owned by: james
mutable description: Alanine-racemase extracted from genomic DNA from E. coli KRX. Used for our biosafety project.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Ligation via Gibson-assembly, so the primer had to have certain extensions
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1172901 (Alanine-Racemase)
sequence: https://synbiohub.org/public/igem/BBa_K1172901_sequence/1

K1172901 (Alanine-Racemase)
type: SequenceAnnotation
persistent identity: annotation2357203
display id: annotation2357203
version: 1
top level: BBa_K1172901
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172901/annotation2357203/range2357203/1

https://synbiohub.org/public/igem/BBa_K1172901/annotation2357203/range2357203/1
type: Range
persistent identity: range2357203
display id: range2357203
version: 1
top level: BBa_K1172901
direction: direction/forward
start: 1
end: 1080
orientation: inline

BBa_K1639008
type: ComponentDefinition
persistent identity: BBa K1639008
display id: BBa_K1639008
version: 1
was generated by: iGEM to SBOL conversion
description: Tev Protease, single S219V mutation in the internal cleavage site
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T07:12:27Z
mutable provenance: Tobacco etch virus (TEV).
owned by: james
owned by: myers
mutable description: TEV protease (also called Tobacco Etch Virus nuclear inclusion a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV).The consensus for these native cut sites is ENLYFQ\S where ???\??? denotes the cleaved peptide bond. One of the main uses of this protein is for removing affinity tags from purified proteins. The reason for the use of TEV protease as a biochemical tool is its high sequence specificity. This specificity allows for the controlled cleavage of proteins when the preference sequence is inserted into flexible loops. It also makes it relatively non-toxic in vivo as the recognized sequence scarcely occurs in proteins.[1]
discontinued: false
dominant: false
experience: experience/Works
group u list: _2056_
m user id: 24789
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part contains an additional BamHI restriction site at beginning of Tev Protease sequence and a XhoI restriction site at the end.
star: false
creator: Mustafa Yılmaz
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: S219V
sequence annotation: ATG Start Codon
sequence annotation: Stop Codon
sequence annotation: Tev Protease
sequence: https://synbiohub.org/public/igem/BBa_K1639008_sequence/1

S219V
type: SequenceAnnotation
persistent identity: annotation2453264
display id: annotation2453264
version: 1
top level: BBa_K1639008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1639008/annotation2453264/range2453264/1

https://synbiohub.org/public/igem/BBa_K1639008/annotation2453264/range2453264/1
type: Range
persistent identity: range2453264
display id: range2453264
version: 1
top level: BBa_K1639008
direction: direction/forward
start: 666
end: 668
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2453252
display id: annotation2453252
version: 1
top level: BBa_K1639008
role: feature/start
role: SO:0000318
location: https://synbiohub.org/public/igem/BBa_K1639008/annotation2453252/range2453252/1

https://synbiohub.org/public/igem/BBa_K1639008/annotation2453252/range2453252/1
type: Range
persistent identity: range2453252
display id: range2453252
version: 1
top level: BBa_K1639008
direction: direction/forward
start: 9
end: 11
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2453254
display id: annotation2453254
version: 1
top level: BBa_K1639008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1639008/annotation2453254/range2453254/1

https://synbiohub.org/public/igem/BBa_K1639008/annotation2453254/range2453254/1
type: Range
persistent identity: range2453254
display id: range2453254
version: 1
top level: BBa_K1639008
direction: direction/forward
start: 738
end: 740
orientation: inline

Tev Protease
type: SequenceAnnotation
persistent identity: annotation2453253
display id: annotation2453253
version: 1
top level: BBa_K1639008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1639008/annotation2453253/range2453253/1

https://synbiohub.org/public/igem/BBa_K1639008/annotation2453253/range2453253/1
type: Range
persistent identity: range2453253
display id: range2453253
version: 1
top level: BBa_K1639008
direction: direction/forward
start: 12
end: 737
orientation: inline

BBa_K914009
type: ComponentDefinition
persistent identity: BBa K914009
display id: BBa_K914009
version: 1
was generated by: iGEM to SBOL conversion
description: P1003* Ser133->Amber Codon
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:46Z
mutable provenance: this part comes from BBa_P1003
owned by: james
owned by: myers
mutable description: The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. Actually it would be better with another mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13470
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: I made the single point mutation with a quick change directed mutagenesis kit.
star: false
creator: Jean Cury
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: kanamycin resistance
sequence annotation: BBa_P1003
sequence annotation: Ser133->Amber codon
sequence: https://synbiohub.org/public/igem/BBa_K914009_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation2212601
display id: annotation2212601
version: 1
top level: BBa_K914009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212601/range2212601/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212601/range2212601/1
type: Range
persistent identity: range2212601
display id: range2212601
version: 1
top level: BBa_K914009
direction: direction/forward
start: 149
end: 967
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2212600
display id: annotation2212600
version: 1
top level: BBa_K914009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212600/range2212600/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212600/range2212600/1
type: Range
persistent identity: range2212600
display id: range2212600
version: 1
top level: BBa_K914009
direction: direction/forward
start: 1
end: 967
orientation: inline

Ser133->Amber codon
type: SequenceAnnotation
persistent identity: annotation2212602
display id: annotation2212602
version: 1
top level: BBa_K914009
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K914009/annotation2212602/range2212602/1

https://synbiohub.org/public/igem/BBa_K914009/annotation2212602/range2212602/1
type: Range
persistent identity: range2212602
display id: range2212602
version: 1
top level: BBa_K914009
direction: direction/forward
start: 546
end: 546
orientation: inline

BBa_K741002
type: ComponentDefinition
persistent identity: BBa K741002
display id: BBa_K741002
version: 1
was generated by: iGEM to SBOL conversion
description: plac-RBS-GFP-T
created: 2012-07-23T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: BBa_R0010 BBa_E0040
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Different strength of green fluorescence will be generated determined by the concentration of IPTG.
discontinued: false
dominant: false
experience: experience/Works
group u list: _990_
m user id: 0
owner id: 11656
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: TBD
star: false
creator: Wuyang Chen
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_I13504
sequence annotation: BBa_I13504
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K741002_sequence/1

BBa_R0010
type: Component
persistent identity: component2177878
display id: component2177878
version: 1
top level: BBa_K741002
access: public
definition: LacI

BBa_I13504
type: Component
persistent identity: component2177894
display id: component2177894
version: 1
top level: BBa_K741002
access: public
definition: BBa_I13504

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2177894
display id: annotation2177894
version: 1
top level: BBa_K741002
location: https://synbiohub.org/public/igem/BBa_K741002/annotation2177894/range2177894/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K741002/annotation2177894/range2177894/1
type: Range
persistent identity: range2177894
display id: range2177894
version: 1
top level: BBa_K741002
direction: direction/reverse
start: 209
end: 1083
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2177878
display id: annotation2177878
version: 1
top level: BBa_K741002
location: https://synbiohub.org/public/igem/BBa_K741002/annotation2177878/range2177878/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K741002/annotation2177878/range2177878/1
type: Range
persistent identity: range2177878
display id: range2177878
version: 1
top level: BBa_K741002
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1774000
type: ComponentDefinition
persistent identity: BBa K1774000
display id: BBa_K1774000
version: 1
was generated by: iGEM to SBOL conversion
description: CRISPR-Cas9 contaiment device repressed by arabinose and tryptophan
creator: HKU iGEM 2015
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:01:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2196_
m user id: 25503
owner id: 25503
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a prototype containment device utilizing CRISPR-Cas9 system which aims to control accidental leakage of GMM once it escape from medium containing arabinose and tryptophan. The device consists of two modules, firstly a arabinose repressible cas9 expression module and a tryptophan repressible gRNA expression module. Under the presence of sufficient amount of arabinose and tryptophan the device remain silent, however when bacteria escape to environment without arabinose and tryptophan, Cas9 and a gRNA targeting DNA polymerase III alpha subunit will be expressed. Subsequent cleavage of the targeted sequence may hinder DNA replication and
mutable notes: a lot
mutable provenance: Cas9 originated from S. pyogenes SF 370
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0015
component: BBa_B0034
component: BBa_K1774002
component: BBa_K1774001
component: BBa_J23106
component: BBa_K1333315
component: BBa_Q04510
component: BBa_K1774003
sequence annotation: BBa_B0034
sequence annotation: BBa_K1774001
sequence annotation: BBa_K1333315
sequence annotation: BBa_K1774002
sequence annotation: BBa_Q04510
sequence annotation: BBa_B0015
sequence annotation: BBa_J23106
sequence annotation: BBa_K1774003
sequence: https://synbiohub.org/public/igem/BBa_K1774000_sequence/1

BBa_B0015
type: Component
persistent identity: component2474085
display id: component2474085
version: 1
top level: BBa_K1774000
access: public
definition: BBa_B0015

BBa_B0034
type: Component
persistent identity: component2474077
display id: component2474077
version: 1
top level: BBa_K1774000
access: public
definition: BBa_B0034

BBa_K1774002
type: Component
persistent identity: component2474102
display id: component2474102
version: 1
top level: BBa_K1774000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1774002/1

BBa_K1774001
type: Component
persistent identity: component2474078
display id: component2474078
version: 1
top level: BBa_K1774000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1774001/1

BBa_J23106
type: Component
persistent identity: component2474086
display id: component2474086
version: 1
top level: BBa_K1774000
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K1333315
type: Component
persistent identity: component2474057
display id: component2474057
version: 1
top level: BBa_K1774000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333315/1

BBa_Q04510
type: Component
persistent identity: component2474075
display id: component2474075
version: 1
top level: BBa_K1774000
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04510/1

BBa_K1774003
type: Component
persistent identity: component2474101
display id: component2474101
version: 1
top level: BBa_K1774000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1774003/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2474077
display id: annotation2474077
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474077/range2474077/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474077/range2474077/1
type: Range
persistent identity: range2474077
display id: range2474077
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 2256
end: 2267
orientation: inline

BBa_K1774001
type: SequenceAnnotation
persistent identity: annotation2474078
display id: annotation2474078
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474078/range2474078/1
component: BBa_K1774001

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474078/range2474078/1
type: Range
persistent identity: range2474078
display id: range2474078
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 2274
end: 6380
orientation: inline

BBa_K1333315
type: SequenceAnnotation
persistent identity: annotation2474057
display id: annotation2474057
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474057/range2474057/1
component: BBa_K1333315

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474057/range2474057/1
type: Range
persistent identity: range2474057
display id: range2474057
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 1
end: 1252
orientation: inline

BBa_K1774002
type: SequenceAnnotation
persistent identity: annotation2474102
display id: annotation2474102
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474102/range2474102/1
component: BBa_K1774002

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474102/range2474102/1
type: Range
persistent identity: range2474102
display id: range2474102
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 7116
end: 7191
orientation: inline

BBa_Q04510
type: SequenceAnnotation
persistent identity: annotation2474075
display id: annotation2474075
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474075/range2474075/1
component: BBa_Q04510

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474075/range2474075/1
type: Range
persistent identity: range2474075
display id: range2474075
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 1261
end: 2247
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2474085
display id: annotation2474085
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474085/range2474085/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474085/range2474085/1
type: Range
persistent identity: range2474085
display id: range2474085
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 6389
end: 6517
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2474086
display id: annotation2474086
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474086/range2474086/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474086/range2474086/1
type: Range
persistent identity: range2474086
display id: range2474086
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 6526
end: 6560
orientation: inline

BBa_K1774003
type: SequenceAnnotation
persistent identity: annotation2474101
display id: annotation2474101
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474101/range2474101/1
component: BBa_K1774003

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474101/range2474101/1
type: Range
persistent identity: range2474101
display id: range2474101
version: 1
direction: direction/reverse
top level: BBa_K1774000
start: 6569
end: 7107
orientation: inline

BBa_K1172904
type: ComponentDefinition
persistent identity: BBa K1172904
display id: BBa_K1172904
version: 1
was generated by: iGEM to SBOL conversion
description: Rnase Ba (Barnase) from Bacillus amyloliquefaciens
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
owned by: james
owned by: myers
mutable description: Rnase Ba (Barnase) from Bacillus Amyloliquefaciens
discontinued: false
dominant: false
experience: experience/None
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1172904 (RNase Ba)
sequence: https://synbiohub.org/public/igem/BBa_K1172904_sequence/1

K1172904 (RNase Ba)
type: SequenceAnnotation
persistent identity: annotation2357200
display id: annotation2357200
version: 1
top level: BBa_K1172904
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172904/annotation2357200/range2357200/1

https://synbiohub.org/public/igem/BBa_K1172904/annotation2357200/range2357200/1
type: Range
persistent identity: range2357200
display id: range2357200
version: 1
top level: BBa_K1172904
direction: direction/forward
start: 1
end: 339
orientation: inline

BBa_K1919100
type: ComponentDefinition
persistent identity: BBa K1919100
display id: BBa_K1919100
version: 1
was generated by: iGEM to SBOL conversion
description: The device consist of temperature promotor and hokD coding gene.
creator: Zekun Lv
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T09:59:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2386_
m user id: 27466
owner id: 27466
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is a combination of two parts submitted by previous igem teams that one （BBa_K608351） is a temperature-regulated promoter followed by the other （BBa_K1497008 ）that a gene coding for a protein hokD which induces cell lysis. The promoter we chose won???t start translation until the temperature is raised to 37℃. And at 42℃ can we get the peck efficiency of expression. The promoter is in fact a composite sequence that preceding the regulated promoter is the control sequence which can synthesis λ cI repressor. λ cI repressor binds with the promoter sequence when environment temperature is 28~30℃ while at 42℃, the protein denatures and falls off by which initiate the transcription. We use the temperature-regulated promoter to express hokD gene. The hokD gene of??E. coli??transcribes for a small polypeptide that causes cell death by elimination of vital cell wall functions. In short , our device can induce cell lysis when temperature is raised to 42℃. To deliver the antimicrobial peptides in the right place and at the right time, we designed and constructed this temperature-regulated device. When put under the sole of feet, our peptide-expressing E.coli cells in the insole would be incubated at a warmer environment due to body temperature. We use this promoter to control the synthesis of hokD for the raised temperature will promote the translation of hokD which following the promoter.
mutable notes: We use pSB1C3 vector to express this device for its more avaliable.
mutable provenance: we acquired that two parts BBa_K608351 and BBa_K1497008 we based on on igem distribution plates and constructed the composite device through molecular biology technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K608351
sequence annotation: BBa_K1497008
sequence: https://synbiohub.org/public/igem/BBa_K1919100_sequence/1

BBa_K608351
type: SequenceAnnotation
persistent identity: annotation2501464
display id: annotation2501464
version: 1
top level: BBa_K1919100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919100/annotation2501464/range2501464/1

https://synbiohub.org/public/igem/BBa_K1919100/annotation2501464/range2501464/1
type: Range
persistent identity: range2501464
display id: range2501464
version: 1
direction: direction/reverse
top level: BBa_K1919100
start: 1
end: 948
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2501707
display id: annotation2501707
version: 1
top level: BBa_K1919100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919100/annotation2501707/range2501707/1

https://synbiohub.org/public/igem/BBa_K1919100/annotation2501707/range2501707/1
type: Range
persistent identity: range2501707
display id: range2501707
version: 1
direction: direction/forward
top level: BBa_K1919100
start: 956
end: 1112
orientation: inline

god
type: ComponentDefinition
persistent identity: BBa K1479011
display id: BBa_K1479011
version: 1
was generated by: iGEM to SBOL conversion
description: oh my god
creator: God
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: long long long long long long long
mutable notes: notes notes notes notes
mutable provenance: source source source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Promoter
sequence annotation: 12
sequence: https://synbiohub.org/public/igem/BBa_K1479011_sequence/1

12
type: SequenceAnnotation
persistent identity: annotation2417085
display id: annotation2417085
version: 1
top level: god
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479011/annotation2417085/range2417085/1

https://synbiohub.org/public/igem/BBa_K1479011/annotation2417085/range2417085/1
type: Range
persistent identity: range2417085
display id: range2417085
version: 1
direction: direction/forward
top level: god
start: 1
end: 2
orientation: inline

BBa_K990005
type: ComponentDefinition
persistent identity: BBa K990005
display id: BBa_K990005
version: 1
was generated by: iGEM to SBOL conversion
description: LasR Device + T4 Lysis Device
creator: Fatma Betul CEVIK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Receiver cell, T4 lysis device is under pLas.
mutable notes: We got BBa_K772004 from 2012 Fatih Medical team.
mutable provenance: LasR Device:K772004 Enterobacteria phage T4 Lysis Device - no promoter: K112808
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K772004
component: BBa_K112808
sequence annotation: BBa_K772004
sequence annotation: BBa_K112808
sequence: https://synbiohub.org/public/igem/BBa_K990005_sequence/1

BBa_K772004
type: Component
persistent identity: component2329973
display id: component2329973
version: 1
top level: BBa_K990005
access: public
definition: https://synbiohub.org/public/igem/BBa_K772004/1

BBa_K112808
type: Component
persistent identity: component2329989
display id: component2329989
version: 1
top level: BBa_K990005
access: public
definition: https://synbiohub.org/public/igem/BBa_K112808/1

BBa_K772004
type: SequenceAnnotation
persistent identity: annotation2329973
display id: annotation2329973
version: 1
top level: BBa_K990005
location: https://synbiohub.org/public/igem/BBa_K990005/annotation2329973/range2329973/1
component: BBa_K772004

https://synbiohub.org/public/igem/BBa_K990005/annotation2329973/range2329973/1
type: Range
persistent identity: range2329973
display id: range2329973
version: 1
direction: direction/reverse
top level: BBa_K990005
start: 1
end: 1122
orientation: inline

BBa_K112808
type: SequenceAnnotation
persistent identity: annotation2329989
display id: annotation2329989
version: 1
top level: BBa_K990005
location: https://synbiohub.org/public/igem/BBa_K990005/annotation2329989/range2329989/1
component: BBa_K112808

https://synbiohub.org/public/igem/BBa_K990005/annotation2329989/range2329989/1
type: Range
persistent identity: range2329989
display id: range2329989
version: 1
direction: direction/reverse
top level: BBa_K990005
start: 1131
end: 2915
orientation: inline

BBa_K1172909
type: ComponentDefinition
persistent identity: BBa K1172909
display id: BBa_K1172909
version: 1
was generated by: iGEM to SBOL conversion
description: Biosafety-System araCtive (AraC)
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
owned by: james
owned by: myers
mutable description: Part 2 of the Biosafety-System L with GFP (dplac GFP)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Tore Bleckwehl
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_B0015 double Terminator
sequence annotation: BBa_K914004 Rhamnose promoter
sequence annotation: arabinose promoter pBAD
sequence annotation: RBS BBa_B0034
sequence annotation: double terminator BBa_B0015
sequence annotation: araC
sequence annotation: BBa_K1172901 alanine racemase (alr)
sequence annotation: GFP BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1172909_sequence/1

BBa_B0015 double Terminator
type: SequenceAnnotation
persistent identity: annotation2370077
display id: annotation2370077
version: 1
top level: BBa_K1172909
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370077/range2370077/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370077/range2370077/1
type: Range
persistent identity: range2370077
display id: range2370077
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 2132
end: 2260
orientation: inline

BBa_K914004 Rhamnose promoter
type: SequenceAnnotation
persistent identity: annotation2370064
display id: annotation2370064
version: 1
top level: BBa_K1172909
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370064/range2370064/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370064/range2370064/1
type: Range
persistent identity: range2370064
display id: range2370064
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 1
end: 122
orientation: inline

arabinose promoter pBAD
type: SequenceAnnotation
persistent identity: annotation2370079
display id: annotation2370079
version: 1
top level: BBa_K1172909
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370079/range2370079/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370079/range2370079/1
type: Range
persistent identity: range2370079
display id: range2370079
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 2269
end: 2398
orientation: inline

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2370067
display id: annotation2370067
version: 1
top level: BBa_K1172909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370067/range2370067/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370067/range2370067/1
type: Range
persistent identity: range2370067
display id: range2370067
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 1029
end: 1040
orientation: inline

double terminator BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2370081
display id: annotation2370081
version: 1
top level: BBa_K1172909
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370081/range2370081/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370081/range2370081/1
type: Range
persistent identity: range2370081
display id: range2370081
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 3153
end: 3281
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2370066
display id: annotation2370066
version: 1
top level: BBa_K1172909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370066/range2370066/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370066/range2370066/1
type: Range
persistent identity: range2370066
display id: range2370066
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 144
end: 1022
orientation: inline

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2370065
display id: annotation2370065
version: 1
top level: BBa_K1172909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370065/range2370065/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370065/range2370065/1
type: Range
persistent identity: range2370065
display id: range2370065
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 129
end: 140
orientation: inline

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2370078
display id: annotation2370078
version: 1
top level: BBa_K1172909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370078/range2370078/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370078/range2370078/1
type: Range
persistent identity: range2370078
display id: range2370078
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 2407
end: 2418
orientation: inline

BBa_K1172901 alanine racemase (alr)
type: SequenceAnnotation
persistent identity: annotation2370068
display id: annotation2370068
version: 1
top level: BBa_K1172909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370068/range2370068/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370068/range2370068/1
type: Range
persistent identity: range2370068
display id: range2370068
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 1044
end: 2123
orientation: inline

GFP BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2370080
display id: annotation2370080
version: 1
top level: BBa_K1172909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370080/range2370080/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370080/range2370080/1
type: Range
persistent identity: range2370080
display id: range2370080
version: 1
top level: BBa_K1172909
direction: direction/forward
start: 2425
end: 3143
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: tetR
sequence annotation: SsrA
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

BBa_K1223001
type: ComponentDefinition
persistent identity: BBa K1223001
display id: BBa_K1223001
version: 1
was generated by: iGEM to SBOL conversion
description: P.A.S.E. 1 cassette
creator: Orr Schlesinger
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A DNA cassette that undergo homologous recombination into E.coli genome that contains homologous regions at each end, Kanamicin resistance gene (KanR) and death operon containing Lambda holin and lysozime genes and controlled by a Lambda cI regulated promoter
mutable notes: /
mutable provenance: part is synthetic. holin and lysozime originates from lambda bacteriophage. the homology regions were taken from E.coli BL21 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: P.A.S.E 1 death operaton
sequence annotation: upstream homology region
sequence annotation: downstream homology region
sequence annotation: Lambda lyzosime
sequence annotation: KanR CDS
sequence annotation: Lambda holin CDS
sequence annotation: cI regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1223001_sequence/1

P.A.S.E 1 death operaton
type: SequenceAnnotation
persistent identity: annotation2336781
display id: annotation2336781
version: 1
top level: BBa_K1223001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1
type: Range
persistent identity: range2336781
display id: range2336781
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 2088
orientation: inline

upstream homology region
type: SequenceAnnotation
persistent identity: annotation2336778
display id: annotation2336778
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1
type: Range
persistent identity: range2336778
display id: range2336778
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 19
end: 73
orientation: inline

downstream homology region
type: SequenceAnnotation
persistent identity: annotation2336784
display id: annotation2336784
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1
type: Range
persistent identity: range2336784
display id: range2336784
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 2094
end: 2148
orientation: inline

Lambda lyzosime
type: SequenceAnnotation
persistent identity: annotation2336782
display id: annotation2336782
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1
type: Range
persistent identity: range2336782
display id: range2336782
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1134
end: 1610
orientation: inline

KanR CDS
type: SequenceAnnotation
persistent identity: annotation2336779
display id: annotation2336779
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1
type: Range
persistent identity: range2336779
display id: range2336779
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 234
end: 1049
orientation: inline

Lambda holin CDS
type: SequenceAnnotation
persistent identity: annotation2336783
display id: annotation2336783
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1
type: Range
persistent identity: range2336783
display id: range2336783
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1631
end: 1948
orientation: inline

cI regulated promoter
type: SequenceAnnotation
persistent identity: annotation2336780
display id: annotation2336780
version: 1
top level: BBa_K1223001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1
type: Range
persistent identity: range2336780
display id: range2336780
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 1107
orientation: inline

BBa_K1554000
type: ComponentDefinition
persistent identity: BBa K1554000
display id: BBa_K1554000
version: 1
was generated by: iGEM to SBOL conversion
description: TA29 promoter
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-29T01:38:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anther-specific promoter
mutable notes: BioBrick compatible
mutable provenance: Genomic from Nicotiana tabacco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1554000_sequence/1

BBa_K914002
type: ComponentDefinition
persistent identity: BBa K914002
display id: BBa_K914002
version: 1
was generated by: iGEM to SBOL conversion
description: repressilator RBS Colicin E2 Immunity protein
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: pDEW Colicin E2
owned by: james
owned by: myers
mutable description: Colicin E2 Immunity protein with the repressilator RBS
discontinued: false
dominant: false
group u list: _1179_
m user id: 0
owner id: 13463
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the full repressilator RBS
star: false
creator: Julianne Rieders
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: BBa_K914002
sequence annotation: repressilator
sequence annotation: cei
sequence: https://synbiohub.org/public/igem/BBa_K914002_sequence/1

BBa_K914002
type: SequenceAnnotation
persistent identity: annotation2187416
display id: annotation2187416
version: 1
top level: BBa_K914002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K914002/annotation2187416/range2187416/1

https://synbiohub.org/public/igem/BBa_K914002/annotation2187416/range2187416/1
type: Range
persistent identity: range2187416
display id: range2187416
version: 1
top level: BBa_K914002
direction: direction/reverse
start: 1
end: 282
orientation: inline

repressilator
type: SequenceAnnotation
persistent identity: annotation2187415
display id: annotation2187415
version: 1
top level: BBa_K914002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K914002/annotation2187415/range2187415/1

https://synbiohub.org/public/igem/BBa_K914002/annotation2187415/range2187415/1
type: Range
persistent identity: range2187415
display id: range2187415
version: 1
top level: BBa_K914002
direction: direction/forward
start: 1
end: 21
orientation: inline

cei
type: SequenceAnnotation
persistent identity: annotation2187417
display id: annotation2187417
version: 1
top level: BBa_K914002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K914002/annotation2187417/range2187417/1

https://synbiohub.org/public/igem/BBa_K914002/annotation2187417/range2187417/1
type: Range
persistent identity: range2187417
display id: range2187417
version: 1
top level: BBa_K914002
direction: direction/forward
start: 22
end: 282
orientation: inline

BBa_K526001
type: ComponentDefinition
persistent identity: BBa K526001
display id: BBa_K526001
version: 1
was generated by: iGEM to SBOL conversion
description: ptrc*-Dpn
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:35Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637214&to=1637675 http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637672&to=1638436
owned by: james
owned by: myers
mutable description: ptrc* is a modified form of ptrc promoter with the -35 and -10 regions being present in the reverse order and the promoter is flanked by fimE recognition sequence. FimE is an invertase capable of inverting any DNA sequence flanked between its recognition seqence. This fim inversion system would help avoid leaky expression. We expressed a new lysis device, DpnI under the control of fim inversion promoter. DpnI is a restriction enzyme capable of chopping up all methylated DNAs. We propose DpnI as a novel lysis device. DpnC and DpnD are obtained from Streptococcus pneumoniae R6 strain.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _692_
m user id: 0
owner id: 8432
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: One should be careful while expressing Dpn genes. Dpn is supposed to chop up the DNA and cause cell death. Hence, no leaky level of Dpn is needed to recover live cells
star: false
creator: Sung Kuk Lee
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DpnC
sequence annotation: DpnD
sequence annotation: fim inversion promoter
sequence: https://synbiohub.org/public/igem/BBa_K526001_sequence/1

DpnC
type: SequenceAnnotation
persistent identity: annotation2150193
display id: annotation2150193
version: 1
top level: BBa_K526001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K526001/annotation2150193/range2150193/1

https://synbiohub.org/public/igem/BBa_K526001/annotation2150193/range2150193/1
type: Range
persistent identity: range2150193
display id: range2150193
version: 1
top level: BBa_K526001
direction: direction/forward
start: 221
end: 1046
orientation: inline

DpnD
type: SequenceAnnotation
persistent identity: annotation2150195
display id: annotation2150195
version: 1
top level: BBa_K526001
role: feature/protein
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K526001/annotation2150195/range2150195/1

https://synbiohub.org/public/igem/BBa_K526001/annotation2150195/range2150195/1
type: Range
persistent identity: range2150195
display id: range2150195
version: 1
top level: BBa_K526001
direction: direction/forward
start: 1077
end: 1536
orientation: inline

fim inversion promoter
type: SequenceAnnotation
persistent identity: annotation2150194
display id: annotation2150194
version: 1
top level: BBa_K526001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K526001/annotation2150194/range2150194/1

https://synbiohub.org/public/igem/BBa_K526001/annotation2150194/range2150194/1
type: Range
persistent identity: range2150194
display id: range2150194
version: 1
top level: BBa_K526001
direction: direction/forward
start: 1
end: 220
orientation: inline

BBa_K515104
type: ComponentDefinition
persistent identity: BBa K515104
display id: BBa_K515104
version: 1
was generated by: iGEM to SBOL conversion
description: J23100 promoter - Antiholin
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: A composite part between J23100 and antiholin
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Atipat Patharagulpong
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K515004
component: BBa_J23100
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K515004
sequence annotation: BBa_J23100
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K515104_sequence/1

BBa_K515004
type: Component
persistent identity: component2129773
display id: component2129773
version: 1
top level: BBa_K515104
access: public
definition: BBa_K515004

BBa_J23100
type: Component
persistent identity: component2129769
display id: component2129769
version: 1
top level: BBa_K515104
access: public
definition: BBa_J23100

BBa_B0010
type: Component
persistent identity: component2129774
display id: component2129774
version: 1
top level: BBa_K515104
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component2129776
display id: component2129776
version: 1
top level: BBa_K515104
access: public
definition: BBa_B0012

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2129774
display id: annotation2129774
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129774/range2129774/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K515104/annotation2129774/range2129774/1
type: Range
persistent identity: range2129774
display id: range2129774
version: 1
top level: BBa_K515104
direction: direction/forward
start: 368
end: 447
orientation: inline

BBa_K515004
type: SequenceAnnotation
persistent identity: annotation2129773
display id: annotation2129773
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129773/range2129773/1
component: BBa_K515004

https://synbiohub.org/public/igem/BBa_K515104/annotation2129773/range2129773/1
type: Range
persistent identity: range2129773
display id: range2129773
version: 1
top level: BBa_K515104
direction: direction/reverse
start: 36
end: 367
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2129769
display id: annotation2129769
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129769/range2129769/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K515104/annotation2129769/range2129769/1
type: Range
persistent identity: range2129769
display id: range2129769
version: 1
top level: BBa_K515104
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2129776
display id: annotation2129776
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129776/range2129776/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K515104/annotation2129776/range2129776/1
type: Range
persistent identity: range2129776
display id: range2129776
version: 1
top level: BBa_K515104
direction: direction/forward
start: 448
end: 488
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0012

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
access: public
definition: GFP

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
top level: BBa_I13504
direction: direction/forward
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
top level: BBa_I13504
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
top level: BBa_I13504
direction: direction/forward
start: 835
end: 875
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
top level: BBa_I13504
direction: direction/forward
start: 747
end: 826
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

test
type: ComponentDefinition
persistent identity: BBa K1479017
display id: BBa_K1479017
version: 1
was generated by: iGEM to SBOL conversion
description: just for test
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:40Z
mutable provenance: fadsfasdfaf
owned by: james
owned by: myers
mutable description: dsfadfadsfa
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21516
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: fafafdfafa
star: false
creator: tester
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: dfdsf
sequence annotation: sdfafds
sequence: https://synbiohub.org/public/igem/BBa_K1479017_sequence/1

dfdsf
type: SequenceAnnotation
persistent identity: annotation2400466
display id: annotation2400466
version: 1
top level: test
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479017/annotation2400466/range2400466/1

https://synbiohub.org/public/igem/BBa_K1479017/annotation2400466/range2400466/1
type: Range
persistent identity: range2400466
display id: range2400466
version: 1
top level: test
direction: direction/forward
start: 2
end: 21
orientation: inline

sdfafds
type: SequenceAnnotation
persistent identity: annotation2400465
display id: annotation2400465
version: 1
top level: test
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1479017/annotation2400465/range2400465/1

https://synbiohub.org/public/igem/BBa_K1479017/annotation2400465/range2400465/1
type: Range
persistent identity: range2400465
display id: range2400465
version: 1
top level: test
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1554004
type: ComponentDefinition
persistent identity: BBa K1554004
display id: BBa_K1554004
version: 1
was generated by: iGEM to SBOL conversion
description: Yellow biosafety module for plants
creator: Valencia_UPV team members
created: 2014-09-28T11:00:00Z
modified: 2015-06-09T09:45:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.
mutable notes: The assembly was performed by GoldenBraid assembly. The barnase was ampliified by PCR from [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced.
mutable provenance: TA29 promoter [http://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] Barnase [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] AmilGFP [Waiting for Norwich team part name in registry] P35s [http://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Barnase
sequence annotation: P35s
sequence annotation: TA29 promoter
sequence annotation: T35s
sequence annotation: AmilGFP
sequence: https://synbiohub.org/public/igem/BBa_K1554004_sequence/1

Barnase
type: SequenceAnnotation
persistent identity: annotation2388986
display id: annotation2388986
version: 1
top level: BBa_K1554004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388986/range2388986/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388986/range2388986/1
type: Range
persistent identity: range2388986
display id: range2388986
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 863
end: 1195
orientation: inline

P35s
type: SequenceAnnotation
persistent identity: annotation2388988
display id: annotation2388988
version: 1
top level: BBa_K1554004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388988/range2388988/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388988/range2388988/1
type: Range
persistent identity: range2388988
display id: range2388988
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 1438
end: 2464
orientation: inline

TA29 promoter
type: SequenceAnnotation
persistent identity: annotation2388985
display id: annotation2388985
version: 1
top level: BBa_K1554004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388985/range2388985/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388985/range2388985/1
type: Range
persistent identity: range2388985
display id: range2388985
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 4
end: 858
orientation: inline

T35s
type: SequenceAnnotation
persistent identity: annotation2388987
display id: annotation2388987
version: 1
top level: BBa_K1554004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388987/range2388987/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388987/range2388987/1
type: Range
persistent identity: range2388987
display id: range2388987
version: 1
direction: direction/reverse
top level: BBa_K1554004
start: 1200
end: 1425
orientation: inline

T35s
type: SequenceAnnotation
persistent identity: annotation2388984
display id: annotation2388984
version: 1
top level: BBa_K1554004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388984/range2388984/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388984/range2388984/1
type: Range
persistent identity: range2388984
display id: range2388984
version: 1
direction: direction/reverse
top level: BBa_K1554004
start: 3166
end: 3391
orientation: inline

AmilGFP
type: SequenceAnnotation
persistent identity: annotation2388989
display id: annotation2388989
version: 1
top level: BBa_K1554004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1554004/annotation2388989/range2388989/1

https://synbiohub.org/public/igem/BBa_K1554004/annotation2388989/range2388989/1
type: Range
persistent identity: range2388989
display id: range2388989
version: 1
direction: direction/forward
top level: BBa_K1554004
start: 2469
end: 3161
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI2
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K628006_sequence/1
type: Sequence
persistent identity: BBa K628006 sequence
display id: BBa_K628006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagacaggacctactagatgcgcggcggccgcctgtgctattgccgccgccgcttttgcgtgtgcgtgggccgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150107_sequence/1
type: Sequence
persistent identity: BBa K150107 sequence
display id: BBa_K150107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914000_sequence/1
type: Sequence
persistent identity: BBa K914000 sequence
display id: BBa_K914000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagcgccgcaaaccccgcttcggcggggtttcgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K515104_sequence/1
type: Sequence
persistent identity: BBa K515104 sequence
display id: BBa_K515104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagccagcctgcggtccgggcgataataaggaggtactaatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2150103_sequence/1
type: Sequence
persistent identity: BBa K2150103 sequence
display id: BBa_K2150103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagttccacgatgtttgatgttatgcaactcaacgaagaacacgatcgtgattcgttctacagtggttcagaatttctagatggttactttcagagacaggttggtcaggatgttaggtgccgcgtagcagcttgttttgttgcgcttcatgatcaccgtgtagcgggatattatactctggctgctgccggtatccagttggccaacctcccgacagctactattaagaaattgccacgatacccaacagtgccggcagtacggatggggaggttggccgttgatcaggcatttagaaatcagggactcggtggagcattgctagctgatgctattgagaggaccattcgtgccgaaattgcatcttatgcgctgcttgttgacgcaaaagatgataatgcagtgcaattttaccgacatcatggttttattgctttacccgattcgccattcacattattccttcctctcatcgctgtagataaaaaaggtaaaaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1223002_sequence/1
type: Sequence
persistent identity: BBa K1223002 sequence
display id: BBa_K1223002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagacgtcacctgggctaccagcccccgctcttgcaattgtttaatctagttactgcttgccatctagtatttctcctctttcgctatctgtgtgaaattgttatccgctcacaattgaatctatcataattgtgagcgctcacaattgtaaaggttagatccgctaatcttatggataaaaatgctatgcagtcccgctcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcttgcagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatcagatcttgatcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttaccagagggcgccccagctggcaattccggttcgcttgcatcttttcagggtgcaagacgatcaatcttccacgcatcattttcacgctgggatcccgggcccgtcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K741002_sequence/1
type: Sequence
persistent identity: BBa K741002 sequence
display id: BBa_K741002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2150104_sequence/1
type: Sequence
persistent identity: BBa K2150104 sequence
display id: BBa_K2150104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgcgcaggctgcgcgtccataacggggttgaagacgacctattcgaggcgttttcctactacgcggacgcggcgccagatcagatcgatcggctttacaacttgtttgtcgatgccgtgacgaagcggattccgcaggctccgaacgcgtttgcgccgttattcaagcactatcgacacatctacctccggcccttcaggtactacgttgcctatcggacgaccgacgaggctatcgacatactggctgttcggcacggaatggagaacccgaacgcggtcgaggctgagatctctggccgcaccttcgagtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150104_sequence/1
type: Sequence
persistent identity: BBa K150104 sequence
display id: BBa_K150104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172911_sequence/1
type: Sequence
persistent identity: BBa K1172911 sequence
display id: BBa_K1172911_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagaaagaggagaaacaaatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataatactagaaagaggagaaacaaatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150008_sequence/1
type: Sequence
persistent identity: BBa K150008 sequence
display id: BBa_K150008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1479017_sequence/1
type: Sequence
persistent identity: BBa K1479017 sequence
display id: BBa_K1479017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgactgatcgacgtcagtcgtacgtacgtacaaagaggagaaatactagagcagctagctagctagctagtcatcgatcgtactgactgatcatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914011_sequence/1
type: Sequence
persistent identity: BBa K914011 sequence
display id: BBa_K914011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagcgccgcaaaccccgcttcggcggggtttcgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2150100_sequence/1
type: Sequence
persistent identity: BBa K2150100 sequence
display id: BBa_K2150100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgtgaaacgcggcgatgtttattttgctgatttatctcctgttgttggctcagagcaaggcggggtgcgcccggttttagtgatccaaaatgacatcggaaatcgcttcagcccaactgctattgttgcagccataacagcacaaatacagaaagcgaaattaccaacccacgtcgaaatcgatgcaaaacgctacggttttgaaagagattccgttattttgctggagcaaattcggacgattgacaagcaaaggttaacggataagattactcatctggatgatgaaatgatggataaggttgatgaagccttacaaatcagtttggcactcattgattttta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150009_sequence/1
type: Sequence
persistent identity: BBa K150009 sequence
display id: BBa_K150009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaggaaacagaaccggatccatggaaaccgcggtagcgtactataaagatggtgttccttatgatgataagggacaggtaattattactcttttgaatggtactcctgacgggagtggctctggcggcggaggtggaaaaggaggcagtaaaagtgaaagttcagcagctattcatgcaactgctaaatggtctactgctcaattaaagaaaacacaggcagagcaggctgcccgggcaaaagcagcagcggaagcacaggcgaaagcaaaggcaaacagggatgcgctgactcagcgcctgaaggatatcgtgaatgaggctcttcgtcacaatgcctcacgtacgccttcagcaacagagcttgctcatgctaataatgcagctatgcaggcggaagcagagcgtttgcgccttgcgaaagcagaagaaaaagcccgtaaagaagcggaagcagcagaaaaggcttttcaggaagcagaacaacgacgtaaagagattgaacgggagaaggctgaaacagaacgccagttgaaactggctgaagctgaagagaaacgactggctgcattgagtgaagaagctaaagctgttgagatcgcccaaaaaaaactttctgctgcacaatctgaagtggtgaaaatggatggagagattaagactctcaattctcgtttaagctccagtatccatgcccgtgatgcagaaatgaaaacgctcgctggaaaacgaaatgaactggctcaggcatccgctaaatataaagaactggatgagctggtcaaaaaactatcaccaagagccaatgatccgcttcagaaccgtcctttttttgaagcaaccagacgacgggttggggccggtaagattagagaagaaaaacaaaaacaggtaacagcatcagaaacacgtattaaccggataaatgctgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K879917_sequence/1
type: Sequence
persistent identity: BBa K879917 sequence
display id: BBa_K879917_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattattgttatccgctcacaatctcaatgcattcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacttgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150005_sequence/1
type: Sequence
persistent identity: BBa K150005 sequence
display id: BBa_K150005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaaacagaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150100_sequence/1
type: Sequence
persistent identity: BBa K150100 sequence
display id: BBa_K150100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K628000_sequence/1
type: Sequence
persistent identity: BBa K628000 sequence
display id: BBa_K628000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgcggcggccgcctgtgctattgccgccgccgcttttgcgtgtgcgtgggccgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1405008_sequence/1
type: Sequence
persistent identity: BBa K1405008 sequence
display id: BBa_K1405008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagctactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172902_sequence/1
type: Sequence
persistent identity: BBa K1172902 sequence
display id: BBa_K1172902_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttgacaattaatcatcggctcgtataatactagagaaagaggagaaatactagatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K131000_sequence/1
type: Sequence
persistent identity: BBa K131000 sequence
display id: BBa_K131000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactcggttttaatcagacctggcatgagtggaagcgggacgaacagcacaggcaacaacaacgccgccccgggcacttccggggcatgagtatgtgatatccggggctgcaccccggaccccgccaacacatcacgggccacaaaattttttgtggcccgctctgcgttttctaagtgttatccctcctgatttctaaaaaattttccacctgaacttgacagaaaaaacgatgacgggtactttttgatctgtacataaaaccagtggttttatgtacagtattaatcatgtaattaattgttttaacgcttaaaagagggaatttttatgagcggtggcgatggacgcggccataacacgggcgcgcatagcacaagtggtaacattaatggtggcccgaccgggcttggtgtaggtggtggtgcttctgatggctccggatggagttcggaaaataacccgtggggtggtggttccggtagcggcattcactggggtggtggttccggtcatggtaatggcggggggaatggtaattccggtggtggttcgggaacaggcggtaatctgtcagcagtagctgcgccagtggcatttggttttccggcactttccactccaggagctggcggtctggcggtcagtatttcagcgggagcattatcggcagctattgctgatattatggctgccctgaaaggaccgtttaaatttggtctttggggggtggctttatatggtgtattgccatcacaaatagcgaaagatgaccccaatatgatgtcaaagattgtgacgtcattacccgcagatgatattactgaatcacctgtcagttcattacctctcgataaggcaacagtaaacgtaaatgttcgtgttgttgatgatgtaaaagacgaacgacagaatatttcggttgtttcaggtgttccgatgagtgttccggtggttgatgcaaaacctaccgaacgtccaggtgtttttacggcatcaattccaggtgcacctgttctgaatatttcagttaataacagtacgccagaagtacagacattaagcccaggtgttacaaataatactgataaggatgttcgcccggcaggatttactcagggtggtaataccagggatgcagttattcgattcccgaaggacagcggtcataatgccgtatatgtttcagtgagtgatgttcttagtcctgaccaggtaaaacaacgtcaggatgaagaaaatcgccgtcagcaggaatgggatgctacgcatccggttgaagcggctgagcgaaattatgaacgcgcgcgtgcagagctgaatcaggcaaatgaagatgttgccagaaatcaggagcgacaggctaaagctgttcaggtttataattcgcgtaaaagcgaacttgatgcagcgaataaaactcttgctgatgcaatagctgaaataaaacaatttaatcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccggacttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaaactttgacgatttccggaagaaattctgggaagaagtgtcaaaagatcccgatcttagtaagcaatttaaaggcagtaataagacgaacattcaaaagggaaaagcaccttttgcaaggaagaaagaccaagtaggtggtagggaacgctttgaattacatcatgataaaccaatcagtcagga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302033_sequence/1
type: Sequence
persistent identity: BBa K302033 sequence
display id: BBa_K302033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtaagccgatacgtacccgatatgggcgatctgatttgggttgattttgacccgacaaaaggtagcgagcaagctggacatcgtccagctgttgtcctgagtcctttcatgtacaacaacaaaacaggtatgtgtctgtgtgttccttgtacaacgcaatcaaaaggatatccgttcgaagttgttttatccggtcaggaacgtgatggcgtagcgttagctgatcaggtaaaaagtatcgcctggcgggcaagaggagcaacgaagaaaggaacagttgccccagaggaattacaactcattaaagccaaaattaacgtactgattgggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914012_sequence/1
type: Sequence
persistent identity: BBa K914012 sequence
display id: BBa_K914012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgctataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914009_sequence/1
type: Sequence
persistent identity: BBa K914009 sequence
display id: BBa_K914009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattagattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1639008_sequence/1
type: Sequence
persistent identity: BBa K1639008 sequence
display id: BBa_K1639008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggatccgatgggcgagtcgctgttcaagggcccgcgtgactacaacccgatttcttctacgatttgtcatttgacaaatgaaagtgatggtcataccaccagcctctacggcattggattcggcccgtttattattacgaacaagcatttatttcgtcgtaataacggcaccctgctcgtgcaatcactccatggcgttttcaaagtcaaaaataccacgacccttcaacagcaccttattgatggtcgtgacatgatcattatccgcatgccgaaagactttccgccatttccgcagaagctcaagtttcgcgaacctcagcgcgaagaacgtatttgcttagtaactaccaattttcagactaaaagtatgagcagcatggtgtccgatacttcctgtacctttccatcatcagatggcattttttggaaacattggattcaaaccaaagacggtcagtgcggtagccctctggtatctacacgcgatggttttattgttggtattcatagcgcttcgaattttactaacaccaacaactactttacgtccgtgccaaaaaacttcatggaactgctgacaaaccaggaagcgcaacagtgggtctcaggttggcgtctgaatgcggattccgttttgtggggtggccacaaggtttttatggtgaaaccggaggaacctttccagccggtcaaggaagccacccagctgatgaatgaactggtgtatagccaataataactcgagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K515004_sequence/1
type: Sequence
persistent identity: BBa K515004 sequence
display id: BBa_K515004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagcctgcggtccgggcgataataaggaggtactaatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K117000_sequence/1
type: Sequence
persistent identity: BBa K117000 sequence
display id: BBa_K117000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgtgatgttcagggcgggacagtatcaccgtcgtcaactgctgaactgaccggagtggaaacgcagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172901_sequence/1
type: Sequence
persistent identity: BBa K1172901 sequence
display id: BBa_K1172901_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172904_sequence/1
type: Sequence
persistent identity: BBa K1172904 sequence
display id: BBa_K1172904_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcagcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagacctttacaaaaatcagataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172909_sequence/1
type: Sequence
persistent identity: BBa K1172909 sequence
display id: BBa_K1172909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagaaagaggagaaacaaatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataatactagaaagaggagaaacaaatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23103_sequence/1
type: Sequence
persistent identity: BBa J23103 sequence
display id: BBa_J23103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088018_sequence/1
type: Sequence
persistent identity: BBa K1088018 sequence
display id: BBa_K1088018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K150007_sequence/1
type: Sequence
persistent identity: BBa K150007 sequence
display id: BBa_K150007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaaaccgcggtagcgtactataaagatggtgttccttatgatgataagggacaggtaattattactcttttgaatggtactcctgacgggagtggctctggcggcggaggtggaaaaggaggcagtaaaagtgaaagttcagcagctattcatgcaactgctaaatggtctactgctcaattaaagaaaacacaggcagagcaggctgcccgggcaaaagcagcagcggaagcacaggcgaaagcaaaggcaaacagggatgcgctgactcagcgcctgaaggatatcgtgaatgaggctcttcgtcacaatgcctcacgtacgccttcagcaacagagcttgctcatgctaataatgcagctatgcaggcggaagcagagcgtttgcgccttgcgaaagcagaagaaaaagcccgtaaagaagcggaagcagcagaaaaggcttttcaggaagcagaacaacgacgtaaagagattgaacgggagaaggctgaaacagaacgccagttgaaactggctgaagctgaagagaaacgactggctgcattgagtgaagaagctaaagctgttgagatcgcccaaaaaaaactttctgctgcacaatctgaagtggtgaaaatggatggagagattaagactctcaattctcgtttaagctccagtatccatgcccgtgatgcagaaatgaaaacgctcgctggaaaacgaaatgaactggctcaggcatccgctaaatataaagaactggatgagctggtcaaaaaactatcaccaagagccaatgatccgcttcagaaccgtcctttttttgaagcaaccagacgacgggttggggccggtaagattagagaagaaaaacaaaaacaggtaacagcatcagaaacacgtattaaccggataaatgctgatataactcagatccagaaggctatttctcaggtcagtaataatcgtaatgccggtatcgctcgtgttcatgaagctgaagaaaatttgaaaaaagcacagaataatctccttaattcacagattaaggatgctgttgatgcaacagttagcttttatcaaacgctgactgaaaaatatggtgaaaaatattcgaaaatggcacaggaacttgctgataagtctaaaggtaagaaaatcggcaatgtgaatgaagctctcgctgcttttgaaaaatacaaggatgttttaaataagaaattcagcaaagccgatcgtgatgctatttttaatgcgttggcatcggtgaagtatgatgactgggctaaacatttagatcagtttgccaagtacttgaagattacggggcatgtttcttttggatatgatgtggtatctgatatcctaaaaattaaggatacaggtgactggaagccactatttcttacattagagaagaaagcagcagatgcaggggtgagttatgttgttgctttactttttagcttgcttgctggaactacattaggtatttggggtattgctattgttacaggtattctatgctcctatattgataagaataaacttaatactataaatgaggtgttagggatttaattattctttattgatataaaaagtcctagcaatccaaatgggattgctaggaccaaacaaagtagattatatagcataaataggtttaattttgctacgggggcgttatttaggttttttcttctttcgaaaaaatctttctttatgaagttaaaagctatgtattcaatagcatattttgaatatggacatagaatagtgcttatcactattgcatatagcatcttatctgacacaaggaaataatacccttcgctgttttttgttataaggtatatatatataagtgtgcagtacaggccaaataaaatattttttatgtagtatcttaagctcataaattaaacctcgccatatattcttttcattttataaggatcgagttatgaggaaaagattttttgtgggaatattcgcgataaacctccttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914001_sequence/1
type: Sequence
persistent identity: BBa K914001 sequence
display id: BBa_K914001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaaggtaccatggaactgaaacatagtattagtgattataccgaggctgaatttctggagtttgtaaaaaaaatatgtagagctgaaggtgctactgaagaggatgacaataaattagtgagagagtttgagcgattaactgagcacccagatggttcagatctgatttattatcctcgcgatgacagggaagatagtcctgaagggattgtcaaggaaattaaagaatggcgagctgctaacggtaagtcaggatttaaacagggctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172908_sequence/1
type: Sequence
persistent identity: BBa K1172908 sequence
display id: BBa_K1172908_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagaaagaggagaaacaaatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataatactagaaagaggagaaacaaatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914002_sequence/1
type: Sequence
persistent identity: BBa K914002 sequence
display id: BBa_K914002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaaggtaccatggaactgaaacatagtattagtgattataccgaggctgaatttctggagtttgtaaaaaaaatatgtagagctgaaggtgctactgaagaggatgacaataaattagtgagagagtttgagcgattaactgagcacccagatggttcagatctgatttattatcctcgcgatgacagggaagatagtcctgaagggattgtcaaggaaattaaagaatggcgagctgctaacggtaagtcaggatttaaacagggctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K526001_sequence/1
type: Sequence
persistent identity: BBa K526001 sequence
display id: BBa_K526001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attgttatctttatttatagatgtttatattgcatgaggtggtttttggagagaagaatgaggaagatgcgtcgagccacagaaacgttagctttacatatagcggaggtgatgtgaaattaatttacaatagaaataatttacatatcaaacagttagatgctttttgtcgttttttaatatttttatggttgacaattaatcatccggctcgtataatatggacagtttggccccaaatgtttcatctttccatggtactagagtcacacaggacggccggatggaattacactttaatttagaattagtagaaacctataaaagtaatagccaaaaggcacgtatcttaacggaggattgggtttatcgtcagtcttattgccctaattgtgggaacaatcccttaaatcattttgaaaataatcggcctgtagcagatttttactgtaatcattgtagtgaggagtttgaactaaagagcaaaaaaggaaatttttcatcaacaatcaatgatggtgcttatgcaacgatgatgaagcgtgtgcaggcagataataatcctaatttcttttttttaacttacacaaaaaattttgaggtaaataactttcttgtccttccgaagcaatttgttacaccgaaatcgattattcaaagaaaaccacttgcaccaactgctagacgagcaggttggattggttgtaacattgatttatcacaagtaccttctaaaggaaggatatttcttgtgcaagatggacaagttagagatccagaaaaagttacaaaagaatttaagcaaggtttatttttaaggaagagctctctgtcatcaagaggttggacaatagaaattctaaattgtatagataagatagagggttcagaatttacccttgaagatatgtatcgttttgaaagtgacctaaaaaatatctttgttaagaacaatcatatcaaagaaaagattaggcaacagcttcaaatattaagagacaaagaaataatagaatttaaaggtagaggaaagtatcggaaattataataatactagagtcacacaggacggccggatgaaaacgaaacaacttgttgcatcagaagaggtgtatgatttcttaaaagtcatctggcctgattatgaaactgaaagccgttacgataacctaagtttaatcgtctgtaccttatcagatcccgattgtgtgagatggttatctgaaaatatgaaatttggtgacgaaaaacaactagctttgatgaaggaaaaatatgggtgggaagtaggagataaattgccagagtggctacatagctcctatcatagattattgttaataggtgaattattggaaagcaatctaaaactgaaaaagtatacagtagaaattacagaaactttatcacgtttagtaagtatagaggctgaaaatccagatgaagccgaacgacttgtaagagaaaagtataagagttgtgaaattgttcttgatgcagatgattttcaggactatgacactagcatatatgaataataagctagcttgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61101_sequence/1
type: Sequence
persistent identity: BBa J61101 sequence
display id: BBa_J61101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagacaggacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K879918_sequence/1
type: Sequence
persistent identity: BBa K879918 sequence
display id: BBa_K879918_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagagtatctctatcactgatagggatgtcaatgcattcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacttgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1318000_sequence/1
type: Sequence
persistent identity: BBa K1318000 sequence
display id: BBa_K1318000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K515106_sequence/1
type: Sequence
persistent identity: BBa K515106 sequence
display id: BBa_K515106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1639022_sequence/1
type: Sequence
persistent identity: BBa K1639022 sequence
display id: BBa_K1639022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggggatccgatgggcgagtcgctgttcaagggcccgcgtgactacaacccgatttcttctacgatttgtcatttgacaaatgaaagtgatggtcataccaccagcctctacggcattggattcggcccgtttattattacgaacaagcatttatttcgtcgtaataacggcaccctgctcgtgcaatcactccatggcgttttcaaagtcaaaaataccacgacccttcaacagcaccttattgatggtcgtgacatgatcattatccgcatgccgaaagactttccgccatttccgcagaagctcaagtttcgcgaacctcagcgcgaagaacgtatttgcttagtaactaccaattttcagactaaaagtatgagcagcatggtgtccgatacttcctgtacctttccatcatcagatggcattttttggaaacattggattcaaaccaaagacggtcagtgcggtagccctctggtatctacacgcgatggttttattgttggtattcatagcgcttcgaattttactaacaccaacaactactttacgtccgtgccaaaaaacttcatggaactgctgacaaaccaggaagcgcaacagtgggtctcaggttggcgtctgaatgcggattccgttttgtggggtggccacaaggtttttatggtgaaaccggaggaacctttccagccggtcaaggaagccacccagctgatgaatgaactggtgtatagccaataataactcgagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13504_sequence/1
type: Sequence
persistent identity: BBa I13504 sequence
display id: BBa_I13504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914018_sequence/1
type: Sequence
persistent identity: BBa K914018 sequence
display id: BBa_K914018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattagattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatagcgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1172912_sequence/1
type: Sequence
persistent identity: BBa K1172912 sequence
display id: BBa_K1172912_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagaaagaggagaaacaaatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataagtactagaaagaggagaaacaaatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z