//biosafety/kill_switch
type: Collection
persistent identity: biosafety kill switch
display id: biosafety_kill_switch
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1172902
member: toxin ParE
member: BBa_K1172909
member: BBa_K879918
member: BBa_K1172912
member: BBa_K879917
member: BBa_K515106
member: BBa_K117000
member: BBa_K1223002
member: BBa_K1639022
member: BBa_K1172901
member: BBa_K515104
member: EndoA
member: BBa_K1919100
member: BBa_K1223001
member: BBa_K1405008
member: BBa_K1172908
member: N-acetyltr
member: CcdB
member: BBa_K1172911
member: BBa_K1184000
member: BBa_K2040122
member: BBa_K628006

CcdB
type: ComponentDefinition
persistent identity: BBa K1318000
display id: BBa_K1318000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CcdB toxin
creator: Laurence Van Melderen
created: 2014-10-01T11:00:00Z
modified: 2015-07-09T11:28:18Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _1693_
m user id: 4206
owner id: 20061
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable description: CcdB is a toxin that poisons and kills E. coli by inhibiting the DNA gyrase and inducing double strand breaks. CcdB activity can be inhibited by the CcdA antitoxin. This gene is under the control of a galactose inducible promoter
mutable notes: CcdB is toxic so it has to be expressed in conjunction with CcdA or not on a galactose-containing medium.
mutable provenance: E. coli fertility factor (F plasmid)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CcdB stop
sequence annotation: CcdB start
sequence annotation: CcdB
sequence: https://synbiohub.org/public/igem/BBa_K1318000_sequence/1

CcdB stop
type: SequenceAnnotation
persistent identity: annotation2416918
display id: annotation2416918
version: 1
top level: CcdB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416918/range2416918/1
type: Range
persistent identity: range2416918
display id: range2416918
version: 1
direction: direction/forward
top level: CcdB
start: 307
end: 309
orientation: inline

CcdB start
type: SequenceAnnotation
persistent identity: annotation2416917
display id: annotation2416917
version: 1
top level: CcdB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416917/range2416917/1
type: Range
persistent identity: range2416917
display id: range2416917
version: 1
direction: direction/forward
top level: CcdB
start: 1
end: 3
orientation: inline

CcdB
type: SequenceAnnotation
persistent identity: annotation2416919
display id: annotation2416919
version: 1
top level: CcdB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1

https://synbiohub.org/public/igem/BBa_K1318000/annotation2416919/range2416919/1
type: Range
persistent identity: range2416919
display id: range2416919
version: 1
direction: direction/forward
top level: CcdB
start: 1
end: 309
orientation: inline

BBa_K1919100
type: ComponentDefinition
persistent identity: BBa K1919100
display id: BBa_K1919100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The device consist of temperature promotor and hokD coding gene.
creator: Zekun Lv
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T09:59:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2386_
m user id: 27466
owner id: 27466
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is a combination of two parts submitted by previous igem teams that one （BBa_K608351） is a temperature-regulated promoter followed by the other （BBa_K1497008 ）that a gene coding for a protein hokD which induces cell lysis. The promoter we chose won???t start translation until the temperature is raised to 37℃. And at 42℃ can we get the peck efficiency of expression. The promoter is in fact a composite sequence that preceding the regulated promoter is the control sequence which can synthesis λ cI repressor. λ cI repressor binds with the promoter sequence when environment temperature is 28~30℃ while at 42℃, the protein denatures and falls off by which initiate the transcription. We use the temperature-regulated promoter to express hokD gene. The hokD gene of??E. coli??transcribes for a small polypeptide that causes cell death by elimination of vital cell wall functions. In short , our device can induce cell lysis when temperature is raised to 42℃. To deliver the antimicrobial peptides in the right place and at the right time, we designed and constructed this temperature-regulated device. When put under the sole of feet, our peptide-expressing E.coli cells in the insole would be incubated at a warmer environment due to body temperature. We use this promoter to control the synthesis of hokD for the raised temperature will promote the translation of hokD which following the promoter.
mutable notes: We use pSB1C3 vector to express this device for its more avaliable.
mutable provenance: we acquired that two parts BBa_K608351 and BBa_K1497008 we based on on igem distribution plates and constructed the composite device through molecular biology technique.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K608351
sequence annotation: BBa_K1497008
sequence: https://synbiohub.org/public/igem/BBa_K1919100_sequence/1

BBa_K608351
type: SequenceAnnotation
persistent identity: annotation2501464
display id: annotation2501464
version: 1
top level: BBa_K1919100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919100/annotation2501464/range2501464/1

https://synbiohub.org/public/igem/BBa_K1919100/annotation2501464/range2501464/1
type: Range
persistent identity: range2501464
display id: range2501464
version: 1
direction: direction/reverse
top level: BBa_K1919100
start: 1
end: 948
orientation: inline

BBa_K1497008
type: SequenceAnnotation
persistent identity: annotation2501707
display id: annotation2501707
version: 1
top level: BBa_K1919100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919100/annotation2501707/range2501707/1

https://synbiohub.org/public/igem/BBa_K1919100/annotation2501707/range2501707/1
type: Range
persistent identity: range2501707
display id: range2501707
version: 1
direction: direction/forward
top level: BBa_K1919100
start: 956
end: 1112
orientation: inline

BBa_K1405008
type: ComponentDefinition
persistent identity: BBa K1405008
display id: BBa_K1405008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A Kill Switch with "memory" time repressed by IPTG
creator: Xiaoyu Yu
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1783_
m user id: 0
owner id: 20576
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: We plan to establish a new E.Coli strain without lacI and MazEF system gene to avoid the inference of cell itself. We will test cI background expression level to see if we need to knock out cI. As is shown in Fig.1, Fig.2, Fig.3 and Fig.4, in the ???Stable State???, before poured into the soil, the E. Coli fertilizer is cultured in the medium with IPTG. Promoter 1 is a weak constitutive promoter, so lacI is transcribed and translated at a considerate low level. Therefore, high concentration of IPTG, which binds LacI and changes LacI???s conformation, is able to inactive LacI and open the promoter 2. Then, CI can be highly expressed to repress promoter3. Finally, mazF is inhibited. After fertilizing, no more IPTG exists in the soil to bind LacI. As a result, LacI with a tag which stabilize the protein takes time to accumulate to a certain high concentration, which is the repression threshold of promoter 2, aiming to represses promoter 2 as well as the expression of cI. The time needed to finish this progress is the designed ???memory time???. During this process, E. coli takes its own responsibility to deliver Mo. And after that, it can be killed to reduce the pollution to environment. As is shown in table 1, all the biobricks in the design are from the top 10 most used parts of iGEM to guarantee the feasibility. Name Number Description CI coding part BBa-C0051 Coding cI Promoter 1 BBa-J23116 Weak constitutive promoter Promoter 2 BBa-R0010 LacI repressible promoter Promoter 3 BBa-R0051 CI repressible promoter Table 1 Biobricks used in the design In the future, we plan to model the ???memory time??? and do more wet-experiments to confirm its feasibility. As for the modeling, we will test the expression level of promoter 1, the minimal stand of LacI to repress promoter 2 and the degradation speed of LacI. On the other hand, we will test the background expression of CI after removing IPTG to check if the CI concentration is low enough to open the promoter 3. Then we wil…
mutable notes: 1
mutable provenance: Name Number Description CI coding part BBa-C0051 Coding cI Promoter 1 BBa-J23116 Weak constitutive promoter Promoter 2 BBa-R0010 LacI repressible promoter Promoter 3 BBa-R0051 CI repressible promoter Table 1 Biobricks used in the design
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K302033
component: BBa_J23116
component: BBa_R0051
component: BBa_K1088018
component: BBa_C0051
component: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_K1088018
sequence annotation: BBa_C0051
sequence annotation: BBa_K302033
sequence annotation: BBa_R0051
sequence annotation: BBa_R0010
sequence annotation: BBa_J23116
sequence: https://synbiohub.org/public/igem/BBa_K1405008_sequence/1

BBa_B0015
type: Component
persistent identity: component2420316
display id: component2420316
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: Component
persistent identity: component2420284
display id: component2420284
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K302033
type: Component
persistent identity: component2420309
display id: component2420309
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_K302033/1

BBa_B0015
type: Component
persistent identity: component2420302
display id: component2420302
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23116
type: Component
persistent identity: component2420276
display id: component2420276
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_J23116/1

BBa_R0051
type: Component
persistent identity: component2420304
display id: component2420304
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_K1088018
type: Component
persistent identity: component2420277
display id: component2420277
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1088018/1

BBa_C0051
type: Component
persistent identity: component2420295
display id: component2420295
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_R0010
type: Component
persistent identity: component2420285
display id: component2420285
version: 1
top level: BBa_K1405008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2420284
display id: annotation2420284
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420284/range2420284/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420284/range2420284/1
type: Range
persistent identity: range2420284
display id: range2420284
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 1133
end: 1261
orientation: inline

BBa_K1088018
type: SequenceAnnotation
persistent identity: annotation2420277
display id: annotation2420277
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420277/range2420277/1
component: BBa_K1088018

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420277/range2420277/1
type: Range
persistent identity: range2420277
display id: range2420277
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 42
end: 1124
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2420295
display id: annotation2420295
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420295/range2420295/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420295/range2420295/1
type: Range
persistent identity: range2420295
display id: range2420295
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 1476
end: 2250
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2420316
display id: annotation2420316
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420316/range2420316/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420316/range2420316/1
type: Range
persistent identity: range2420316
display id: range2420316
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 2798
end: 2926
orientation: inline

BBa_K302033
type: SequenceAnnotation
persistent identity: annotation2420309
display id: annotation2420309
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420309/range2420309/1
component: BBa_K302033

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420309/range2420309/1
type: Range
persistent identity: range2420309
display id: range2420309
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 2451
end: 2789
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2420304
display id: annotation2420304
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420304/range2420304/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420304/range2420304/1
type: Range
persistent identity: range2420304
display id: range2420304
version: 1
direction: direction/forward
top level: BBa_K1405008
start: 2396
end: 2444
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2420302
display id: annotation2420302
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420302/range2420302/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420302/range2420302/1
type: Range
persistent identity: range2420302
display id: range2420302
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 2259
end: 2387
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2420285
display id: annotation2420285
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420285/range2420285/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420285/range2420285/1
type: Range
persistent identity: range2420285
display id: range2420285
version: 1
direction: direction/forward
top level: BBa_K1405008
start: 1270
end: 1469
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2420276
display id: annotation2420276
version: 1
top level: BBa_K1405008
location: https://synbiohub.org/public/igem/BBa_K1405008/annotation2420276/range2420276/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K1405008/annotation2420276/range2420276/1
type: Range
persistent identity: range2420276
display id: range2420276
version: 1
direction: direction/reverse
top level: BBa_K1405008
start: 1
end: 35
orientation: inline

BBa_K1223001
type: ComponentDefinition
persistent identity: BBa K1223001
display id: BBa_K1223001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P.A.S.E. 1 cassette
creator: Orr Schlesinger
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A DNA cassette that undergo homologous recombination into E.coli genome that contains homologous regions at each end, Kanamicin resistance gene (KanR) and death operon containing Lambda holin and lysozime genes and controlled by a Lambda cI regulated promoter
mutable notes: /
mutable provenance: part is synthetic. holin and lysozime originates from lambda bacteriophage. the homology regions were taken from E.coli BL21 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Lambda holin CDS
sequence annotation: KanR CDS
sequence annotation: P.A.S.E 1 death operaton
sequence annotation: upstream homology region
sequence annotation: Lambda lyzosime
sequence annotation: downstream homology region
sequence annotation: cI regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_K1223001_sequence/1

Lambda holin CDS
type: SequenceAnnotation
persistent identity: annotation2336783
display id: annotation2336783
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336783/range2336783/1
type: Range
persistent identity: range2336783
display id: range2336783
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1631
end: 1948
orientation: inline

KanR CDS
type: SequenceAnnotation
persistent identity: annotation2336779
display id: annotation2336779
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336779/range2336779/1
type: Range
persistent identity: range2336779
display id: range2336779
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 234
end: 1049
orientation: inline

P.A.S.E 1 death operaton
type: SequenceAnnotation
persistent identity: annotation2336781
display id: annotation2336781
version: 1
top level: BBa_K1223001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336781/range2336781/1
type: Range
persistent identity: range2336781
display id: range2336781
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 2088
orientation: inline

upstream homology region
type: SequenceAnnotation
persistent identity: annotation2336778
display id: annotation2336778
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336778/range2336778/1
type: Range
persistent identity: range2336778
display id: range2336778
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 19
end: 73
orientation: inline

Lambda lyzosime
type: SequenceAnnotation
persistent identity: annotation2336782
display id: annotation2336782
version: 1
top level: BBa_K1223001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336782/range2336782/1
type: Range
persistent identity: range2336782
display id: range2336782
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1134
end: 1610
orientation: inline

downstream homology region
type: SequenceAnnotation
persistent identity: annotation2336784
display id: annotation2336784
version: 1
top level: BBa_K1223001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336784/range2336784/1
type: Range
persistent identity: range2336784
display id: range2336784
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 2094
end: 2148
orientation: inline

cI regulated promoter
type: SequenceAnnotation
persistent identity: annotation2336780
display id: annotation2336780
version: 1
top level: BBa_K1223001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1

https://synbiohub.org/public/igem/BBa_K1223001/annotation2336780/range2336780/1
type: Range
persistent identity: range2336780
display id: range2336780
version: 1
direction: direction/forward
top level: BBa_K1223001
start: 1059
end: 1107
orientation: inline

BBa_K1172908
type: ComponentDefinition
persistent identity: BBa K1172908
display id: BBa_K1172908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr)
creator: Tore Bleckwehl
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part 1 of the Biosafety-System L (pRha lacI alr)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: lacI-LVA (C0012)
sequence annotation: Alanine-Racemase (K1172901)
sequence annotation: double Terminator (B0015)
sequence annotation: pRha (K914004)
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1172908_sequence/1

lacI-LVA (C0012)
type: SequenceAnnotation
persistent identity: annotation2359483
display id: annotation2359483
version: 1
top level: BBa_K1172908
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359483/range2359483/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359483/range2359483/1
type: Range
persistent identity: range2359483
display id: range2359483
version: 1
direction: direction/forward
top level: BBa_K1172908
start: 144
end: 1271
orientation: inline

Alanine-Racemase (K1172901)
type: SequenceAnnotation
persistent identity: annotation2359485
display id: annotation2359485
version: 1
top level: BBa_K1172908
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359485/range2359485/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359485/range2359485/1
type: Range
persistent identity: range2359485
display id: range2359485
version: 1
direction: direction/forward
top level: BBa_K1172908
start: 1293
end: 2372
orientation: inline

double Terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2359486
display id: annotation2359486
version: 1
top level: BBa_K1172908
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359486/range2359486/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359486/range2359486/1
type: Range
persistent identity: range2359486
display id: range2359486
version: 1
direction: direction/forward
top level: BBa_K1172908
start: 2381
end: 2509
orientation: inline

pRha (K914004)
type: SequenceAnnotation
persistent identity: annotation2355542
display id: annotation2355542
version: 1
top level: BBa_K1172908
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2355542/range2355542/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2355542/range2355542/1
type: Range
persistent identity: range2355542
display id: range2355542
version: 1
direction: direction/forward
top level: BBa_K1172908
start: 1
end: 122
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2359484
display id: annotation2359484
version: 1
top level: BBa_K1172908
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359484/range2359484/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359484/range2359484/1
type: Range
persistent identity: range2359484
display id: range2359484
version: 1
direction: direction/forward
top level: BBa_K1172908
start: 1278
end: 1289
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2359482
display id: annotation2359482
version: 1
top level: BBa_K1172908
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172908/annotation2359482/range2359482/1

https://synbiohub.org/public/igem/BBa_K1172908/annotation2359482/range2359482/1
type: Range
persistent identity: range2359482
display id: range2359482
version: 1
direction: direction/forward
top level: BBa_K1172908
start: 129
end: 140
orientation: inline

BBa_K1172911
type: ComponentDefinition
persistent identity: BBa K1172911
display id: BBa_K1172911
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosafety-System Lac of Growth (lacI)
creator: Tore Bleckwehl
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Biosafety-System L (lacI)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1172908
component: BBa_K741002
sequence annotation: BBa_K1172908
sequence annotation: BBa_K741002
sequence: https://synbiohub.org/public/igem/BBa_K1172911_sequence/1

BBa_K1172908
type: Component
persistent identity: component2359493
display id: component2359493
version: 1
top level: BBa_K1172911
access: public
definition: BBa_K1172908

BBa_K741002
type: Component
persistent identity: component2359511
display id: component2359511
version: 1
top level: BBa_K1172911
access: public
definition: https://synbiohub.org/public/igem/BBa_K741002/1

BBa_K1172908
type: SequenceAnnotation
persistent identity: annotation2359493
display id: annotation2359493
version: 1
top level: BBa_K1172911
location: https://synbiohub.org/public/igem/BBa_K1172911/annotation2359493/range2359493/1
component: BBa_K1172908

https://synbiohub.org/public/igem/BBa_K1172911/annotation2359493/range2359493/1
type: Range
persistent identity: range2359493
display id: range2359493
version: 1
direction: direction/reverse
top level: BBa_K1172911
start: 1
end: 2509
orientation: inline

BBa_K741002
type: SequenceAnnotation
persistent identity: annotation2359511
display id: annotation2359511
version: 1
top level: BBa_K1172911
location: https://synbiohub.org/public/igem/BBa_K1172911/annotation2359511/range2359511/1
component: BBa_K741002

https://synbiohub.org/public/igem/BBa_K1172911/annotation2359511/range2359511/1
type: Range
persistent identity: range2359511
display id: range2359511
version: 1
direction: direction/reverse
top level: BBa_K1172911
start: 2518
end: 3600
orientation: inline

BBa_K515104
type: ComponentDefinition
persistent identity: BBa K515104
display id: BBa_K515104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 promoter - Antiholin
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part between J23100 and antiholin
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_K515004
component: BBa_J23100
sequence annotation: BBa_K515004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K515104_sequence/1

BBa_B0010
type: Component
persistent identity: component2129774
display id: component2129774
version: 1
top level: BBa_K515104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2129776
display id: component2129776
version: 1
top level: BBa_K515104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K515004
type: Component
persistent identity: component2129773
display id: component2129773
version: 1
top level: BBa_K515104
access: public
definition: https://synbiohub.org/public/igem/BBa_K515004/1

BBa_J23100
type: Component
persistent identity: component2129769
display id: component2129769
version: 1
top level: BBa_K515104
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K515004
type: SequenceAnnotation
persistent identity: annotation2129773
display id: annotation2129773
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129773/range2129773/1
component: BBa_K515004

https://synbiohub.org/public/igem/BBa_K515104/annotation2129773/range2129773/1
type: Range
persistent identity: range2129773
display id: range2129773
version: 1
direction: direction/reverse
top level: BBa_K515104
start: 36
end: 367
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2129774
display id: annotation2129774
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129774/range2129774/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K515104/annotation2129774/range2129774/1
type: Range
persistent identity: range2129774
display id: range2129774
version: 1
direction: direction/forward
top level: BBa_K515104
start: 368
end: 447
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2129776
display id: annotation2129776
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129776/range2129776/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K515104/annotation2129776/range2129776/1
type: Range
persistent identity: range2129776
display id: range2129776
version: 1
direction: direction/forward
top level: BBa_K515104
start: 448
end: 488
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2129769
display id: annotation2129769
version: 1
top level: BBa_K515104
location: https://synbiohub.org/public/igem/BBa_K515104/annotation2129769/range2129769/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K515104/annotation2129769/range2129769/1
type: Range
persistent identity: range2129769
display id: range2129769
version: 1
direction: direction/reverse
top level: BBa_K515104
start: 1
end: 35
orientation: inline

toxin ParE
type: ComponentDefinition
persistent identity: BBa K2150104
display id: BBa_K2150104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 6249 toxin CDS
creator: Zepeng Mu
created: 2016-10-11T11:00:00Z
modified: 2016-10-13T11:15:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ParD2
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K2150104_sequence/1

ParD2
type: SequenceAnnotation
persistent identity: annotation2499113
display id: annotation2499113
version: 1
top level: toxin ParE
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2150104/annotation2499113/range2499113/1

https://synbiohub.org/public/igem/BBa_K2150104/annotation2499113/range2499113/1
type: Range
persistent identity: range2499113
display id: range2499113
version: 1
direction: direction/forward
top level: toxin ParE
start: 1
end: 316
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2499115
display id: annotation2499115
version: 1
top level: toxin ParE
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2150104/annotation2499115/range2499115/1

https://synbiohub.org/public/igem/BBa_K2150104/annotation2499115/range2499115/1
type: Range
persistent identity: range2499115
display id: range2499115
version: 1
direction: direction/forward
top level: toxin ParE
start: 317
end: 319
orientation: inline

BBa_K879917
type: ComponentDefinition
persistent identity: BBa K879917
display id: BBa_K879917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacO ori
creator: Thomas Patrick
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: lacO ori
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: BioBrick prefix
sequence annotation: T0 terminator
sequence annotation: E coli his operon terminator
sequence annotation: VF2
sequence annotation: -10
sequence annotation: BioBrick suffix
sequence annotation: RNA II
sequence annotation: pMB1 derived replication origin
sequence annotation: -35
sequence annotation: Chloramphenicol resistance
sequence annotation: LacO operator
sequence: https://synbiohub.org/public/igem/BBa_K879917_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2210377
display id: annotation2210377
version: 1
top level: BBa_K879917
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210377/range2210377/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210377/range2210377/1
type: Range
persistent identity: range2210377
display id: range2210377
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 159
end: 178
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210342
display id: annotation2210342
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210342/range2210342/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210342/range2210342/1
type: Range
persistent identity: range2210342
display id: range2210342
version: 1
direction: direction/forward
top level: BBa_K879917
start: 2053
end: 2072
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210379
display id: annotation2210379
version: 1
top level: BBa_K879917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210379/range2210379/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210379/range2210379/1
type: Range
persistent identity: range2210379
display id: range2210379
version: 1
direction: direction/forward
top level: BBa_K879917
start: 1044
end: 1149
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210376
display id: annotation2210376
version: 1
top level: BBa_K879917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210376/range2210376/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210376/range2210376/1
type: Range
persistent identity: range2210376
display id: range2210376
version: 1
direction: direction/forward
top level: BBa_K879917
start: 22
end: 95
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2210382
display id: annotation2210382
version: 1
top level: BBa_K879917
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210382/range2210382/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210382/range2210382/1
type: Range
persistent identity: range2210382
display id: range2210382
version: 1
direction: direction/forward
top level: BBa_K879917
start: 1935
end: 1954
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210566
display id: annotation2210566
version: 1
top level: BBa_K879917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210566/range2210566/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210566/range2210566/1
type: Range
persistent identity: range2210566
display id: range2210566
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 836
end: 841
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210341
display id: annotation2210341
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210341/range2210341/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210341/range2210341/1
type: Range
persistent identity: range2210341
display id: range2210341
version: 1
direction: direction/forward
top level: BBa_K879917
start: 1
end: 21
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210383
display id: annotation2210383
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210383/range2210383/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210383/range2210383/1
type: Range
persistent identity: range2210383
display id: range2210383
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 277
end: 826
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210378
display id: annotation2210378
version: 1
top level: BBa_K879917
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210378/range2210378/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210378/range2210378/1
type: Range
persistent identity: range2210378
display id: range2210378
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 263
end: 877
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2210568
display id: annotation2210568
version: 1
top level: BBa_K879917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210568/range2210568/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210568/range2210568/1
type: Range
persistent identity: range2210568
display id: range2210568
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 859
end: 864
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210380
display id: annotation2210380
version: 1
top level: BBa_K879917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210380/range2210380/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210380/range2210380/1
type: Range
persistent identity: range2210380
display id: range2210380
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 1162
end: 1821
orientation: inline

LacO operator
type: SequenceAnnotation
persistent identity: annotation2210567
display id: annotation2210567
version: 1
top level: BBa_K879917
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879917/annotation2210567/range2210567/1

https://synbiohub.org/public/igem/BBa_K879917/annotation2210567/range2210567/1
type: Range
persistent identity: range2210567
display id: range2210567
version: 1
direction: direction/reverse
top level: BBa_K879917
start: 842
end: 858
orientation: inline

BBa_K2040122
type: ComponentDefinition
persistent identity: BBa K2040122
display id: BBa_K2040122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed + NLS
creator: Chilam Poon
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T01:35:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2507_
m user id: 30360
owner id: 30360
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A SV40 nuclear localization signal was added to the phototoxic protein KillerRed.
mutable notes: NLS
mutable provenance: BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KillerRed
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K2040122_sequence/1

KillerRed
type: SequenceAnnotation
persistent identity: annotation2518117
display id: annotation2518117
version: 1
top level: BBa_K2040122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1
type: Range
persistent identity: range2518117
display id: range2518117
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 1
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2502105
display id: annotation2502105
version: 1
top level: BBa_K2040122
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1
type: Range
persistent identity: range2502105
display id: range2502105
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 718
end: 741
orientation: inline

BBa_K1172909
type: ComponentDefinition
persistent identity: BBa K1172909
display id: BBa_K1172909
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosafety-System araCtive (AraC)
creator: Tore Bleckwehl
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Part 2 of the Biosafety-System L with GFP (dplac GFP)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_B0015 double Terminator
sequence annotation: GFP BBa_E0040
sequence annotation: arabinose promoter pBAD
sequence annotation: RBS BBa_B0034
sequence annotation: double terminator BBa_B0015
sequence annotation: BBa_K1172901 alanine racemase (alr)
sequence annotation: araC
sequence annotation: BBa_K914004 Rhamnose promoter
sequence: https://synbiohub.org/public/igem/BBa_K1172909_sequence/1

BBa_B0015 double Terminator
type: SequenceAnnotation
persistent identity: annotation2370077
display id: annotation2370077
version: 1
top level: BBa_K1172909
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370077/range2370077/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370077/range2370077/1
type: Range
persistent identity: range2370077
display id: range2370077
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 2132
end: 2260
orientation: inline

GFP BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2370080
display id: annotation2370080
version: 1
top level: BBa_K1172909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370080/range2370080/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370080/range2370080/1
type: Range
persistent identity: range2370080
display id: range2370080
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 2425
end: 3143
orientation: inline

arabinose promoter pBAD
type: SequenceAnnotation
persistent identity: annotation2370079
display id: annotation2370079
version: 1
top level: BBa_K1172909
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370079/range2370079/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370079/range2370079/1
type: Range
persistent identity: range2370079
display id: range2370079
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 2269
end: 2398
orientation: inline

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2370067
display id: annotation2370067
version: 1
top level: BBa_K1172909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370067/range2370067/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370067/range2370067/1
type: Range
persistent identity: range2370067
display id: range2370067
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 1029
end: 1040
orientation: inline

double terminator BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2370081
display id: annotation2370081
version: 1
top level: BBa_K1172909
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370081/range2370081/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370081/range2370081/1
type: Range
persistent identity: range2370081
display id: range2370081
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 3153
end: 3281
orientation: inline

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2370065
display id: annotation2370065
version: 1
top level: BBa_K1172909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370065/range2370065/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370065/range2370065/1
type: Range
persistent identity: range2370065
display id: range2370065
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 129
end: 140
orientation: inline

BBa_K1172901 alanine racemase (alr)
type: SequenceAnnotation
persistent identity: annotation2370068
display id: annotation2370068
version: 1
top level: BBa_K1172909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370068/range2370068/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370068/range2370068/1
type: Range
persistent identity: range2370068
display id: range2370068
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 1044
end: 2123
orientation: inline

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2370078
display id: annotation2370078
version: 1
top level: BBa_K1172909
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370078/range2370078/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370078/range2370078/1
type: Range
persistent identity: range2370078
display id: range2370078
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 2407
end: 2418
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2370066
display id: annotation2370066
version: 1
top level: BBa_K1172909
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370066/range2370066/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370066/range2370066/1
type: Range
persistent identity: range2370066
display id: range2370066
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 144
end: 1022
orientation: inline

BBa_K914004 Rhamnose promoter
type: SequenceAnnotation
persistent identity: annotation2370064
display id: annotation2370064
version: 1
top level: BBa_K1172909
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172909/annotation2370064/range2370064/1

https://synbiohub.org/public/igem/BBa_K1172909/annotation2370064/range2370064/1
type: Range
persistent identity: range2370064
display id: range2370064
version: 1
direction: direction/forward
top level: BBa_K1172909
start: 1
end: 122
orientation: inline

BBa_K1639022
type: ComponentDefinition
persistent identity: BBa K1639022
display id: BBa_K1639022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCons-TetR-pTet-TevProtease Suicide Switch
creator: Mustafa Yılmaz
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T10:18:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2056_
m user id: 20835
owner id: 20835
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a kill switch part of our engineering E. Coli. pTet is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. In the presence of aTc TetR repression inhibited and Tev Protease produce by Ptet promoter. Tev Protease release Pexiganan from DAMP and E. Coli kill switch works succesfully.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_J23100
component: BBa_R0040
component: BBa_K1639008
sequence annotation: BBa_R0040
sequence annotation: BBa_K1639008
sequence annotation: BBa_C0040
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1639022_sequence/1

BBa_C0040
type: Component
persistent identity: component2472055
display id: component2472055
version: 1
top level: BBa_K1639022
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J23100
type: Component
persistent identity: component2472051
display id: component2472051
version: 1
top level: BBa_K1639022
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_R0040
type: Component
persistent identity: component2472056
display id: component2472056
version: 1
top level: BBa_K1639022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1639008
type: Component
persistent identity: component2472065
display id: component2472065
version: 1
top level: BBa_K1639022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1639008/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2472056
display id: annotation2472056
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472056/range2472056/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472056/range2472056/1
type: Range
persistent identity: range2472056
display id: range2472056
version: 1
direction: direction/forward
top level: BBa_K1639022
start: 735
end: 788
orientation: inline

BBa_K1639008
type: SequenceAnnotation
persistent identity: annotation2472065
display id: annotation2472065
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472065/range2472065/1
component: BBa_K1639008

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472065/range2472065/1
type: Range
persistent identity: range2472065
display id: range2472065
version: 1
direction: direction/reverse
top level: BBa_K1639022
start: 797
end: 1546
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2472055
display id: annotation2472055
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472055/range2472055/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472055/range2472055/1
type: Range
persistent identity: range2472055
display id: range2472055
version: 1
direction: direction/reverse
top level: BBa_K1639022
start: 42
end: 726
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2472051
display id: annotation2472051
version: 1
top level: BBa_K1639022
location: https://synbiohub.org/public/igem/BBa_K1639022/annotation2472051/range2472051/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1639022/annotation2472051/range2472051/1
type: Range
persistent identity: range2472051
display id: range2472051
version: 1
direction: direction/reverse
top level: BBa_K1639022
start: 1
end: 35
orientation: inline

EndoA
type: ComponentDefinition
persistent identity: BBa K2150100
display id: BBa_K2150100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 134 toxin CDS
creator: Zepeng Mu
created: 2016-10-11T11:00:00Z
modified: 2016-10-13T09:53:36Z
discontinued: true
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2150100_sequence/1

BBa_K1172901
type: ComponentDefinition
persistent identity: BBa K1172901
display id: BBa_K1172901
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alanine racemase from ''E. coli''
creator: Tore Bleckwehl
created: 2013-08-25T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16785
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Alanine-racemase extracted from genomic DNA from E. coli KRX. Used for our biosafety project.
mutable notes: Ligation via Gibson-assembly, so the primer had to have certain extensions
mutable provenance: E. coli KRX genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1172901 (Alanine-Racemase)
sequence: https://synbiohub.org/public/igem/BBa_K1172901_sequence/1

K1172901 (Alanine-Racemase)
type: SequenceAnnotation
persistent identity: annotation2357203
display id: annotation2357203
version: 1
top level: BBa_K1172901
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172901/annotation2357203/range2357203/1

https://synbiohub.org/public/igem/BBa_K1172901/annotation2357203/range2357203/1
type: Range
persistent identity: range2357203
display id: range2357203
version: 1
direction: direction/forward
top level: BBa_K1172901
start: 1
end: 1080
orientation: inline

BBa_K1184000
type: ComponentDefinition
persistent identity: BBa K1184000
display id: BBa_K1184000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed
creator: Eric Pederson
created: 2013-09-03T11:00:00Z
modified: 2016-01-25T02:40:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1497_
m user id: 4206
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates. This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
mutable notes: This sequence is not codon optimized for E. coli.
mutable provenance: Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Phototoxic Mutation Asn(147)
sequence annotation: Phototoxic Mutation Glu(70)
sequence annotation: Phototoxic Mutation Ser(121)
sequence annotation: Double Stop
sequence annotation: Start
sequence annotation: Chromophore
sequence annotation: cds
sequence annotation: Phototoxic Mutation Ala(163)
sequence: https://synbiohub.org/public/igem/BBa_K1184000_sequence/1

Phototoxic Mutation Asn(147)
type: SequenceAnnotation
persistent identity: annotation2334960
display id: annotation2334960
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1
type: Range
persistent identity: range2334960
display id: range2334960
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 439
end: 441
orientation: inline

Phototoxic Mutation Glu(70)
type: SequenceAnnotation
persistent identity: annotation2334956
display id: annotation2334956
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1
type: Range
persistent identity: range2334956
display id: range2334956
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 208
end: 210
orientation: inline

Phototoxic Mutation Ser(121)
type: SequenceAnnotation
persistent identity: annotation2334957
display id: annotation2334957
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1
type: Range
persistent identity: range2334957
display id: range2334957
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 367
end: 369
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2334953
display id: annotation2334953
version: 1
top level: BBa_K1184000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1
type: Range
persistent identity: range2334953
display id: range2334953
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 718
end: 723
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2334954
display id: annotation2334954
version: 1
top level: BBa_K1184000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1
type: Range
persistent identity: range2334954
display id: range2334954
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 3
orientation: inline

Chromophore
type: SequenceAnnotation
persistent identity: annotation2334958
display id: annotation2334958
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1
type: Range
persistent identity: range2334958
display id: range2334958
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 199
end: 207
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2334955
display id: annotation2334955
version: 1
top level: BBa_K1184000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1
type: Range
persistent identity: range2334955
display id: range2334955
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 717
orientation: inline

Phototoxic Mutation Ala(163)
type: SequenceAnnotation
persistent identity: annotation2334965
display id: annotation2334965
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1
type: Range
persistent identity: range2334965
display id: range2334965
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 487
end: 489
orientation: inline

N-acetyltr
type: ComponentDefinition
persistent identity: BBa K2150103
display id: BBa_K2150103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1204 toxin CDS
creator: Zepeng Mu
created: 2016-10-11T11:00:00Z
modified: 2016-10-18T06:28:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2620_
m user id: 29734
owner id: 29734
owning group id: 9
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CDS of N-acetyltransferase GCN5
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2150103_sequence/1

CDS of N-acetyltransferase GCN5
type: SequenceAnnotation
persistent identity: annotation2519779
display id: annotation2519779
version: 1
top level: N-acetyltr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2150103/annotation2519779/range2519779/1

https://synbiohub.org/public/igem/BBa_K2150103/annotation2519779/range2519779/1
type: Range
persistent identity: range2519779
display id: range2519779
version: 1
direction: direction/forward
top level: N-acetyltr
start: 1
end: 501
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2519780
display id: annotation2519780
version: 1
top level: N-acetyltr
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2150103/annotation2519780/range2519780/1

https://synbiohub.org/public/igem/BBa_K2150103/annotation2519780/range2519780/1
type: Range
persistent identity: range2519780
display id: range2519780
version: 1
direction: direction/forward
top level: N-acetyltr
start: 502
end: 506
orientation: inline

BBa_K1223002
type: ComponentDefinition
persistent identity: BBa K1223002
display id: BBa_K1223002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P.A.S.E. 2 cassette
creator: Orr Schlesinger
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1537_
m user id: 0
owner id: 18143
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: kanamicin ressistance gene (promoter+CDS)
mutable notes: selection for cassette integration into E.coli BL21 genome
mutable provenance: yeast
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: start codon
sequence annotation: stop codon (TAG)
sequence annotation: stop codon
sequence annotation: RBS
sequence annotation: KanR
sequence annotation: AraC\Lac promoter
sequence annotation: Neo/Kan promoter
sequence annotation: TyrS Homology region
sequence annotation: KanR CDS
sequence annotation: homology region
sequence: https://synbiohub.org/public/igem/BBa_K1223002_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2337653
display id: annotation2337653
version: 1
top level: BBa_K1223002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337653/range2337653/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337653/range2337653/1
type: Range
persistent identity: range2337653
display id: range2337653
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 62
end: 64
orientation: inline

stop codon (TAG)
type: SequenceAnnotation
persistent identity: annotation2337652
display id: annotation2337652
version: 1
top level: BBa_K1223002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337652/range2337652/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337652/range2337652/1
type: Range
persistent identity: range2337652
display id: range2337652
version: 1
direction: direction/forward
top level: BBa_K1223002
start: 47
end: 49
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2337654
display id: annotation2337654
version: 1
top level: BBa_K1223002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337654/range2337654/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337654/range2337654/1
type: Range
persistent identity: range2337654
display id: range2337654
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 200
end: 202
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2337646
display id: annotation2337646
version: 1
top level: BBa_K1223002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337646/range2337646/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337646/range2337646/1
type: Range
persistent identity: range2337646
display id: range2337646
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 71
end: 81
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation2337647
display id: annotation2337647
version: 1
top level: BBa_K1223002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337647/range2337647/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337647/range2337647/1
type: Range
persistent identity: range2337647
display id: range2337647
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 200
end: 1132
orientation: inline

AraC\Lac promoter
type: SequenceAnnotation
persistent identity: annotation2337645
display id: annotation2337645
version: 1
top level: BBa_K1223002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337645/range2337645/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337645/range2337645/1
type: Range
persistent identity: range2337645
display id: range2337645
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 87
end: 191
orientation: inline

Neo/Kan promoter
type: SequenceAnnotation
persistent identity: annotation2337650
display id: annotation2337650
version: 1
top level: BBa_K1223002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337650/range2337650/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337650/range2337650/1
type: Range
persistent identity: range2337650
display id: range2337650
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 1083
end: 1132
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2337655
display id: annotation2337655
version: 1
top level: BBa_K1223002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337655/range2337655/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337655/range2337655/1
type: Range
persistent identity: range2337655
display id: range2337655
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 992
end: 994
orientation: inline

TyrS Homology region
type: SequenceAnnotation
persistent identity: annotation2337644
display id: annotation2337644
version: 1
top level: BBa_K1223002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337644/range2337644/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337644/range2337644/1
type: Range
persistent identity: range2337644
display id: range2337644
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 5
end: 64
orientation: inline

KanR CDS
type: SequenceAnnotation
persistent identity: annotation2337648
display id: annotation2337648
version: 1
top level: BBa_K1223002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337648/range2337648/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337648/range2337648/1
type: Range
persistent identity: range2337648
display id: range2337648
version: 1
direction: direction/reverse
top level: BBa_K1223002
start: 200
end: 994
orientation: inline

homology region
type: SequenceAnnotation
persistent identity: annotation2337651
display id: annotation2337651
version: 1
top level: BBa_K1223002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1223002/annotation2337651/range2337651/1

https://synbiohub.org/public/igem/BBa_K1223002/annotation2337651/range2337651/1
type: Range
persistent identity: range2337651
display id: range2337651
version: 1
direction: direction/forward
top level: BBa_K1223002
start: 1140
end: 1192
orientation: inline

BBa_K117000
type: ComponentDefinition
persistent identity: BBa K117000
display id: BBa_K117000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis gene (promotes lysis in colicin-producing bacteria strain)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K117000_sequence/1

BBa_K628006
type: ComponentDefinition
persistent identity: BBa K628006
display id: BBa_K628006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protegrin-1 Kill Switch
creator: Charles Thompson
created: 2011-09-19T11:00:00Z
modified: 2016-02-01T12:54:32Z
discontinued: false
dominant: false
group u list: _800_
m user id: 4206
owner id: 9738
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K206000
component: BBa_K628000
component: BBa_J61101
component: BBa_B0015
sequence annotation: BBa_J61101
sequence annotation: BBa_K628000
sequence annotation: BBa_B0015
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K628006_sequence/1

BBa_K206000
type: Component
persistent identity: component2135980
display id: component2135980
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K628000
type: Component
persistent identity: component2135982
display id: component2135982
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_K628000/1

BBa_J61101
type: Component
persistent identity: component2135981
display id: component2135981
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_B0015
type: Component
persistent identity: component2135989
display id: component2135989
version: 1
top level: BBa_K628006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2135981
display id: annotation2135981
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135981/range2135981/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K628006/annotation2135981/range2135981/1
type: Range
persistent identity: range2135981
display id: range2135981
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 139
end: 150
orientation: inline

BBa_K628000
type: SequenceAnnotation
persistent identity: annotation2135982
display id: annotation2135982
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135982/range2135982/1
component: BBa_K628000

https://synbiohub.org/public/igem/BBa_K628006/annotation2135982/range2135982/1
type: Range
persistent identity: range2135982
display id: range2135982
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 157
end: 216
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2135989
display id: annotation2135989
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135989/range2135989/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K628006/annotation2135989/range2135989/1
type: Range
persistent identity: range2135989
display id: range2135989
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 225
end: 353
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2135980
display id: annotation2135980
version: 1
top level: BBa_K628006
location: https://synbiohub.org/public/igem/BBa_K628006/annotation2135980/range2135980/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K628006/annotation2135980/range2135980/1
type: Range
persistent identity: range2135980
display id: range2135980
version: 1
direction: direction/reverse
top level: BBa_K628006
start: 1
end: 130
orientation: inline

BBa_K1172902
type: ComponentDefinition
persistent identity: BBa K1172902
display id: BBa_K1172902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alanine racemase (''alr'') under the control of the P''tac'' promoter
creator: Tore Bleckwehl
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Alanin_Racemase (alr) under the control of the ptac-Promoter
mutable notes: Complementation of D-Alanine-Auxotrophy
mutable provenance: Genom Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: K1172901 (Alanine-Racemase)
sequence annotation: ptac-promoter
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1172902_sequence/1

K1172901 (Alanine-Racemase)
type: SequenceAnnotation
persistent identity: annotation2357202
display id: annotation2357202
version: 1
top level: BBa_K1172902
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172902/annotation2357202/range2357202/1

https://synbiohub.org/public/igem/BBa_K1172902/annotation2357202/range2357202/1
type: Range
persistent identity: range2357202
display id: range2357202
version: 1
direction: direction/forward
top level: BBa_K1172902
start: 55
end: 1134
orientation: inline

ptac-promoter
type: SequenceAnnotation
persistent identity: annotation2355534
display id: annotation2355534
version: 1
top level: BBa_K1172902
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172902/annotation2355534/range2355534/1

https://synbiohub.org/public/igem/BBa_K1172902/annotation2355534/range2355534/1
type: Range
persistent identity: range2355534
display id: range2355534
version: 1
direction: direction/forward
top level: BBa_K1172902
start: 1
end: 28
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2357201
display id: annotation2357201
version: 1
top level: BBa_K1172902
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172902/annotation2357201/range2357201/1

https://synbiohub.org/public/igem/BBa_K1172902/annotation2357201/range2357201/1
type: Range
persistent identity: range2357201
display id: range2357201
version: 1
direction: direction/forward
top level: BBa_K1172902
start: 37
end: 48
orientation: inline

BBa_K879918
type: ComponentDefinition
persistent identity: BBa K879918
display id: BBa_K879918
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetO ori
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: TetO ori
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick suffix
sequence annotation: -35
sequence annotation: T0 terminator
sequence annotation: pMB1 derived replication origin
sequence annotation: VR
sequence annotation: RNA II
sequence annotation: TetO operator
sequence annotation: -10
sequence annotation: VF2
sequence annotation: BioBrick prefix
sequence annotation: Chloramphenicol resistance
sequence annotation: E coli his operon terminator
sequence: https://synbiohub.org/public/igem/BBa_K879918_sequence/1

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2210749
display id: annotation2210749
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210749/range2210749/1
type: Range
persistent identity: range2210749
display id: range2210749
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1
end: 21
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2210756
display id: annotation2210756
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210756/range2210756/1
type: Range
persistent identity: range2210756
display id: range2210756
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 859
end: 864
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2210757
display id: annotation2210757
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210757/range2210757/1
type: Range
persistent identity: range2210757
display id: range2210757
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1044
end: 1149
orientation: inline

pMB1 derived replication origin
type: SequenceAnnotation
persistent identity: annotation2210752
display id: annotation2210752
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210752/range2210752/1
type: Range
persistent identity: range2210752
display id: range2210752
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 263
end: 877
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2210751
display id: annotation2210751
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210751/range2210751/1
type: Range
persistent identity: range2210751
display id: range2210751
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 159
end: 178
orientation: inline

RNA II
type: SequenceAnnotation
persistent identity: annotation2210753
display id: annotation2210753
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210753/range2210753/1
type: Range
persistent identity: range2210753
display id: range2210753
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 277
end: 826
orientation: inline

TetO operator
type: SequenceAnnotation
persistent identity: annotation2210755
display id: annotation2210755
version: 1
top level: BBa_K879918
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210755/range2210755/1
type: Range
persistent identity: range2210755
display id: range2210755
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 842
end: 858
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2210754
display id: annotation2210754
version: 1
top level: BBa_K879918
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210754/range2210754/1
type: Range
persistent identity: range2210754
display id: range2210754
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 836
end: 841
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2210759
display id: annotation2210759
version: 1
top level: BBa_K879918
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210759/range2210759/1
type: Range
persistent identity: range2210759
display id: range2210759
version: 1
direction: direction/forward
top level: BBa_K879918
start: 1935
end: 1954
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2210775
display id: annotation2210775
version: 1
top level: BBa_K879918
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210775/range2210775/1
type: Range
persistent identity: range2210775
display id: range2210775
version: 1
direction: direction/forward
top level: BBa_K879918
start: 2053
end: 2072
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2210758
display id: annotation2210758
version: 1
top level: BBa_K879918
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210758/range2210758/1
type: Range
persistent identity: range2210758
display id: range2210758
version: 1
direction: direction/reverse
top level: BBa_K879918
start: 1162
end: 1821
orientation: inline

E coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2210750
display id: annotation2210750
version: 1
top level: BBa_K879918
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1

https://synbiohub.org/public/igem/BBa_K879918/annotation2210750/range2210750/1
type: Range
persistent identity: range2210750
display id: range2210750
version: 1
direction: direction/forward
top level: BBa_K879918
start: 22
end: 95
orientation: inline

BBa_K515106
type: ComponentDefinition
persistent identity: BBa K515106
display id: BBa_K515106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806
creator: Atipat Patharagulpong
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A composite part comprising of a J23100 promotor and PA2652 translational unit encoding extracellular chemoreceptor
mutable notes: -
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K112805
component: BBa_J23103
component: BBa_K112806
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_K112805
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_K112806
sequence annotation: BBa_J23103
sequence: https://synbiohub.org/public/igem/BBa_K515106_sequence/1

BBa_K112805
type: Component
persistent identity: component2258432
display id: component2258432
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_K112805/1

BBa_J23103
type: Component
persistent identity: component2258425
display id: component2258425
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K112806
type: Component
persistent identity: component2258434
display id: component2258434
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_K112806/1

BBa_B0034
type: Component
persistent identity: component2258427
display id: component2258427
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2258430
display id: component2258430
version: 1
top level: BBa_K515106
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2258432
display id: annotation2258432
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258432/range2258432/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K515106/annotation2258432/range2258432/1
type: Range
persistent identity: range2258432
display id: range2258432
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 776
end: 1451
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2258427
display id: annotation2258427
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258427/range2258427/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K515106/annotation2258427/range2258427/1
type: Range
persistent identity: range2258427
display id: range2258427
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 44
end: 55
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2258430
display id: annotation2258430
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258430/range2258430/1
component: BBa_E1010

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type: Range
persistent identity: range2258430
display id: range2258430
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 62
end: 767
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2258434
display id: annotation2258434
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258434/range2258434/1
component: BBa_K112806

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type: Range
persistent identity: range2258434
display id: range2258434
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 1460
end: 1973
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2258425
display id: annotation2258425
version: 1
top level: BBa_K515106
location: https://synbiohub.org/public/igem/BBa_K515106/annotation2258425/range2258425/1
component: BBa_J23103

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type: Range
persistent identity: range2258425
display id: range2258425
version: 1
direction: direction/reverse
top level: BBa_K515106
start: 1
end: 35
orientation: inline

BBa_K1172912
type: ComponentDefinition
persistent identity: BBa K1172912
display id: BBa_K1172912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Part 1 of the Biosystem TetOR alive (pRha TetR alr)
creator: Tore Bleckwehl
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1485_
m user id: 0
owner id: 16986
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part 1 of the Biosystem M (pRha TetR alr)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: K914004 Rhamnose promotor
sequence annotation: B0034
sequence annotation: K1172901 alanine racemase
sequence annotation: B0015 double terminator
sequence annotation: TetR C0040
sequence: https://synbiohub.org/public/igem/BBa_K1172912_sequence/1

K914004 Rhamnose promotor
type: SequenceAnnotation
persistent identity: annotation2371125
display id: annotation2371125
version: 1
top level: BBa_K1172912
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371125/range2371125/1

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type: Range
persistent identity: range2371125
display id: range2371125
version: 1
direction: direction/forward
top level: BBa_K1172912
start: 1
end: 122
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2371126
display id: annotation2371126
version: 1
top level: BBa_K1172912
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371126/range2371126/1

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type: Range
persistent identity: range2371126
display id: range2371126
version: 1
direction: direction/forward
top level: BBa_K1172912
start: 129
end: 140
orientation: inline

K1172901 alanine racemase
type: SequenceAnnotation
persistent identity: annotation2371129
display id: annotation2371129
version: 1
top level: BBa_K1172912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371129/range2371129/1

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type: Range
persistent identity: range2371129
display id: range2371129
version: 1
direction: direction/forward
top level: BBa_K1172912
start: 826
end: 1905
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2371128
display id: annotation2371128
version: 1
top level: BBa_K1172912
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371128/range2371128/1

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type: Range
persistent identity: range2371128
display id: range2371128
version: 1
direction: direction/forward
top level: BBa_K1172912
start: 811
end: 822
orientation: inline

B0015 double terminator
type: SequenceAnnotation
persistent identity: annotation2371130
display id: annotation2371130
version: 1
top level: BBa_K1172912
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371130/range2371130/1

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type: Range
persistent identity: range2371130
display id: range2371130
version: 1
direction: direction/forward
top level: BBa_K1172912
start: 1914
end: 2042
orientation: inline

TetR C0040
type: SequenceAnnotation
persistent identity: annotation2371127
display id: annotation2371127
version: 1
top level: BBa_K1172912
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1172912/annotation2371127/range2371127/1

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type: Range
persistent identity: range2371127
display id: range2371127
version: 1
direction: direction/forward
top level: BBa_K1172912
start: 144
end: 803
orientation: inline