//cds/biosynthesis/anthocyanins
type: Collection
persistent identity: cds biosynthesis anthocyanins
display id: cds_biosynthesis_anthocyanins
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1497003
member: BBa_K1497006
member: BBa_K1497017
member: BBa_K1497004
member: BBa_K1497011
member: BBa_K1497013
member: BBa_K1497000
member: BBa_K1497015
member: BBa_K1497002
member: BBa_K1497010
member: BBa_K1497009
member: BBa_K1497023
member: BBa_K1497007
member: BBa_K1497005
member: BBa_K1497012
member: BBa_K1497018
member: BBa_K1497001
member: BBa_K1497016
member: BBa_K1497014

BBa_K1497010
type: ComponentDefinition
persistent identity: BBa K1497010
display id: BBa_K1497010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dihydroflavonol 4-reductase (DFR)
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497010_sequence/1

BBa_K1497018
type: ComponentDefinition
persistent identity: BBa K1497018
display id: BBa_K1497018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-F3H-B0034-DFR
creator: Sascha Hein, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: s
mutable notes: s
mutable provenance: s
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497012
component: BBa_K1497013
sequence annotation: BBa_K1497012
sequence annotation: BBa_K1497013
sequence: https://synbiohub.org/public/igem/BBa_K1497018_sequence/1

BBa_K1497012
type: Component
persistent identity: component2398934
display id: component2398934
version: 1
top level: BBa_K1497018
access: public
definition: BBa_K1497012

BBa_K1497013
type: Component
persistent identity: component2398938
display id: component2398938
version: 1
top level: BBa_K1497018
access: public
definition: BBa_K1497013

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2398934
display id: annotation2398934
version: 1
top level: BBa_K1497018
location: https://synbiohub.org/public/igem/BBa_K1497018/annotation2398934/range2398934/1
component: BBa_K1497012

https://synbiohub.org/public/igem/BBa_K1497018/annotation2398934/range2398934/1
type: Range
persistent identity: range2398934
display id: range2398934
version: 1
direction: direction/reverse
top level: BBa_K1497018
start: 1
end: 1125
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2398938
display id: annotation2398938
version: 1
top level: BBa_K1497018
location: https://synbiohub.org/public/igem/BBa_K1497018/annotation2398938/range2398938/1
component: BBa_K1497013

https://synbiohub.org/public/igem/BBa_K1497018/annotation2398938/range2398938/1
type: Range
persistent identity: range2398938
display id: range2398938
version: 1
direction: direction/reverse
top level: BBa_K1497018
start: 1134
end: 2213
orientation: inline

BBa_K1497003
type: ComponentDefinition
persistent identity: BBa K1497003
display id: BBa_K1497003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-CHI - Chalcone Isomerase from Petunia with strong RBS
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-10T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
mutable notes: no
mutable provenance: Petunia
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1497000
sequence annotation: BBa_K1497000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1497003_sequence/1

BBa_B0034
type: Component
persistent identity: component2416907
display id: component2416907
version: 1
top level: BBa_K1497003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497000
type: Component
persistent identity: component2416908
display id: component2416908
version: 1
top level: BBa_K1497003
access: public
definition: BBa_K1497000

BBa_K1497000
type: SequenceAnnotation
persistent identity: annotation2416908
display id: annotation2416908
version: 1
top level: BBa_K1497003
location: https://synbiohub.org/public/igem/BBa_K1497003/annotation2416908/range2416908/1
component: BBa_K1497000

https://synbiohub.org/public/igem/BBa_K1497003/annotation2416908/range2416908/1
type: Range
persistent identity: range2416908
display id: range2416908
version: 1
direction: direction/reverse
top level: BBa_K1497003
start: 19
end: 744
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2416907
display id: annotation2416907
version: 1
top level: BBa_K1497003
location: https://synbiohub.org/public/igem/BBa_K1497003/annotation2416907/range2416907/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497003/annotation2416907/range2416907/1
type: Range
persistent identity: range2416907
display id: range2416907
version: 1
direction: direction/reverse
top level: BBa_K1497003
start: 1
end: 12
orientation: inline

BBa_K1497005
type: ComponentDefinition
persistent identity: BBa K1497005
display id: BBa_K1497005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-CHS-B0034-CHI - Composite part of RBS-CHS and RBS-CHI
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device is able to generate Naringenin from 4-courmaroyl-CoA, if put under a promotor. This device is the second composite part for the assembly of our Naringenin Operon (K1497007). Part 1: K1497004: B0034-CHS - Chalcone Synthase from Gerbera with strong RBS The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034. Part 2: K1497003: B0034-CHI - Chalcone Isomerase from Petunia with strong RBS The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
mutable notes: no
mutable provenance: Petunia and Gerbara
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497004
component: BBa_K1497003
sequence annotation: BBa_K1497004
sequence annotation: BBa_K1497003
sequence: https://synbiohub.org/public/igem/BBa_K1497005_sequence/1

BBa_K1497004
type: Component
persistent identity: component2416916
display id: component2416916
version: 1
top level: BBa_K1497005
access: public
definition: BBa_K1497004

BBa_K1497003
type: Component
persistent identity: component2416912
display id: component2416912
version: 1
top level: BBa_K1497005
access: public
definition: BBa_K1497003

BBa_K1497004
type: SequenceAnnotation
persistent identity: annotation2416916
display id: annotation2416916
version: 1
top level: BBa_K1497005
location: https://synbiohub.org/public/igem/BBa_K1497005/annotation2416916/range2416916/1
component: BBa_K1497004

https://synbiohub.org/public/igem/BBa_K1497005/annotation2416916/range2416916/1
type: Range
persistent identity: range2416916
display id: range2416916
version: 1
direction: direction/reverse
top level: BBa_K1497005
start: 753
end: 1967
orientation: inline

BBa_K1497003
type: SequenceAnnotation
persistent identity: annotation2416912
display id: annotation2416912
version: 1
top level: BBa_K1497005
location: https://synbiohub.org/public/igem/BBa_K1497005/annotation2416912/range2416912/1
component: BBa_K1497003

https://synbiohub.org/public/igem/BBa_K1497005/annotation2416912/range2416912/1
type: Range
persistent identity: range2416912
display id: range2416912
version: 1
direction: direction/reverse
top level: BBa_K1497005
start: 1
end: 744
orientation: inline

BBa_K1497011
type: ComponentDefinition
persistent identity: BBa K1497011
display id: BBa_K1497011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-ANS
creator: Sascha Hein, Sven Jager, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497011_sequence/1

BBa_K1497004
type: ComponentDefinition
persistent identity: BBa K1497004
display id: BBa_K1497004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-CHS - Chalcone Synthase from Gerbera with strong RBS
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
mutable notes: no
mutable provenance: Gerbera
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1497004_sequence/1

BBa_B0034
type: Component
persistent identity: component2398582
display id: component2398582
version: 1
top level: BBa_K1497004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398582
display id: annotation2398582
version: 1
top level: BBa_K1497004
location: https://synbiohub.org/public/igem/BBa_K1497004/annotation2398582/range2398582/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497004/annotation2398582/range2398582/1
type: Range
persistent identity: range2398582
display id: range2398582
version: 1
direction: direction/reverse
top level: BBa_K1497004
start: 1
end: 12
orientation: inline

BBa_K1497007
type: ComponentDefinition
persistent identity: BBa K1497007
display id: BBa_K1497007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin producing operon
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes: 4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497100) CHS (Bba_K1497101) For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.
mutable notes: no
mutable provenance: The functionality of this construct is verified by putting the device under a promotor. Proofing functionality leaded to Bba_K1497008 (Naringenin Operon under the constitutive promotor J23100) and Bba_K1497008 (Naringenin Operon under the T7 Promotor).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K801093
component: BBa_K1497000
component: BBa_K1497001
component: BBa_K1033000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1033000
sequence annotation: BBa_K801093
sequence annotation: BBa_K1497001
sequence annotation: BBa_K1497000
sequence: https://synbiohub.org/public/igem/BBa_K1497007_sequence/1

BBa_B0034
type: Component
persistent identity: component2398753
display id: component2398753
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2398747
display id: component2398747
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K801093
type: Component
persistent identity: component2398751
display id: component2398751
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_K801093/1

BBa_B0034
type: Component
persistent identity: component2398761
display id: component2398761
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497000
type: Component
persistent identity: component2398759
display id: component2398759
version: 1
top level: BBa_K1497007
access: public
definition: BBa_K1497000

BBa_B0034
type: Component
persistent identity: component2398758
display id: component2398758
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497001
type: Component
persistent identity: component2398762
display id: component2398762
version: 1
top level: BBa_K1497007
access: public
definition: BBa_K1497001

BBa_K1033000
type: Component
persistent identity: component2398756
display id: component2398756
version: 1
top level: BBa_K1497007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033000/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398747
display id: annotation2398747
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398747/range2398747/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398747/range2398747/1
type: Range
persistent identity: range2398747
display id: range2398747
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 1
end: 12
orientation: inline

BBa_K1033000
type: SequenceAnnotation
persistent identity: annotation2398756
display id: annotation2398756
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398756/range2398756/1
component: BBa_K1033000

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398756/range2398756/1
type: Range
persistent identity: range2398756
display id: range2398756
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 1733
end: 3328
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398761
display id: annotation2398761
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398761/range2398761/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398761/range2398761/1
type: Range
persistent identity: range2398761
display id: range2398761
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 4089
end: 4100
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398758
display id: annotation2398758
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398758/range2398758/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398758/range2398758/1
type: Range
persistent identity: range2398758
display id: range2398758
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 3337
end: 3348
orientation: inline

BBa_K801093
type: SequenceAnnotation
persistent identity: annotation2398751
display id: annotation2398751
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398751/range2398751/1
component: BBa_K801093

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398751/range2398751/1
type: Range
persistent identity: range2398751
display id: range2398751
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 19
end: 1704
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398753
display id: annotation2398753
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398753/range2398753/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398753/range2398753/1
type: Range
persistent identity: range2398753
display id: range2398753
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 1713
end: 1724
orientation: inline

BBa_K1497001
type: SequenceAnnotation
persistent identity: annotation2398762
display id: annotation2398762
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398762/range2398762/1
component: BBa_K1497001

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398762/range2398762/1
type: Range
persistent identity: range2398762
display id: range2398762
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 4107
end: 5303
orientation: inline

BBa_K1497000
type: SequenceAnnotation
persistent identity: annotation2398759
display id: annotation2398759
version: 1
top level: BBa_K1497007
location: https://synbiohub.org/public/igem/BBa_K1497007/annotation2398759/range2398759/1
component: BBa_K1497000

https://synbiohub.org/public/igem/BBa_K1497007/annotation2398759/range2398759/1
type: Range
persistent identity: range2398759
display id: range2398759
version: 1
direction: direction/reverse
top level: BBa_K1497007
start: 3355
end: 4080
orientation: inline

BBa_K1497023
type: ComponentDefinition
persistent identity: BBa K1497023
display id: BBa_K1497023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pelargonidin producing operon B0034-F3H-B0034-DFR-B0034-eANS
creator: Sascha Hein, Sebastian Barthel, Thomas Dohmen, Sven Jager
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1497013
component: BBa_K1497012
component: BBa_K1497002
sequence annotation: BBa_K1497012
sequence annotation: BBa_K1497002
sequence annotation: BBa_K1497013
sequence: https://synbiohub.org/public/igem/BBa_K1497023_sequence/1

BBa_K1497013
type: Component
persistent identity: component2399036
display id: component2399036
version: 1
top level: BBa_K1497023
access: public
definition: BBa_K1497013

BBa_K1497012
type: Component
persistent identity: component2399032
display id: component2399032
version: 1
top level: BBa_K1497023
access: public
definition: BBa_K1497012

BBa_K1497002
type: Component
persistent identity: component2399037
display id: component2399037
version: 1
top level: BBa_K1497023
access: public
definition: BBa_K1497002

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2399032
display id: annotation2399032
version: 1
top level: BBa_K1497023
location: https://synbiohub.org/public/igem/BBa_K1497023/annotation2399032/range2399032/1
component: BBa_K1497012

https://synbiohub.org/public/igem/BBa_K1497023/annotation2399032/range2399032/1
type: Range
persistent identity: range2399032
display id: range2399032
version: 1
direction: direction/reverse
top level: BBa_K1497023
start: 1
end: 1125
orientation: inline

BBa_K1497002
type: SequenceAnnotation
persistent identity: annotation2399037
display id: annotation2399037
version: 1
top level: BBa_K1497023
location: https://synbiohub.org/public/igem/BBa_K1497023/annotation2399037/range2399037/1
component: BBa_K1497002

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type: Range
persistent identity: range2399037
display id: range2399037
version: 1
direction: direction/reverse
top level: BBa_K1497023
start: 2222
end: 3189
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2399036
display id: annotation2399036
version: 1
top level: BBa_K1497023
location: https://synbiohub.org/public/igem/BBa_K1497023/annotation2399036/range2399036/1
component: BBa_K1497013

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type: Range
persistent identity: range2399036
display id: range2399036
version: 1
direction: direction/reverse
top level: BBa_K1497023
start: 1134
end: 2213
orientation: inline

BBa_K1497013
type: ComponentDefinition
persistent identity: BBa K1497013
display id: BBa_K1497013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-DFR
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1497010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1497010
sequence: https://synbiohub.org/public/igem/BBa_K1497013_sequence/1

BBa_B0034
type: Component
persistent identity: component2398836
display id: component2398836
version: 1
top level: BBa_K1497013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497010
type: Component
persistent identity: component2398837
display id: component2398837
version: 1
top level: BBa_K1497013
access: public
definition: BBa_K1497010

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398836
display id: annotation2398836
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398836/range2398836/1
component: BBa_B0034

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type: Range
persistent identity: range2398836
display id: range2398836
version: 1
direction: direction/reverse
top level: BBa_K1497013
start: 1
end: 12
orientation: inline

BBa_K1497010
type: SequenceAnnotation
persistent identity: annotation2398837
display id: annotation2398837
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398837/range2398837/1
component: BBa_K1497010

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type: Range
persistent identity: range2398837
display id: range2398837
version: 1
direction: direction/reverse
top level: BBa_K1497013
start: 19
end: 1080
orientation: inline

BBa_K1497000
type: ComponentDefinition
persistent identity: BBa K1497000
display id: BBa_K1497000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chalcone Isomerase (CHI) from Petunia
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin. Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin. We used the CHI and verified the function in our Naringenin Operon (K1497007).
mutable notes: Original Sequence from Petunia
mutable provenance: Petunia Hybrida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497000_sequence/1

BBa_K1497016
type: ComponentDefinition
persistent identity: BBa K1497016
display id: BBa_K1497016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive naringenin producing operon
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1497007
component: BBa_J23100
sequence annotation: BBa_K1497007
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1497016_sequence/1

BBa_K1497007
type: Component
persistent identity: component2398929
display id: component2398929
version: 1
top level: BBa_K1497016
access: public
definition: BBa_K1497007

BBa_J23100
type: Component
persistent identity: component2398911
display id: component2398911
version: 1
top level: BBa_K1497016
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1497007
type: SequenceAnnotation
persistent identity: annotation2398929
display id: annotation2398929
version: 1
top level: BBa_K1497016
location: https://synbiohub.org/public/igem/BBa_K1497016/annotation2398929/range2398929/1
component: BBa_K1497007

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type: Range
persistent identity: range2398929
display id: range2398929
version: 1
direction: direction/reverse
top level: BBa_K1497016
start: 44
end: 5346
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2398911
display id: annotation2398911
version: 1
top level: BBa_K1497016
location: https://synbiohub.org/public/igem/BBa_K1497016/annotation2398911/range2398911/1
component: BBa_J23100

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type: Range
persistent identity: range2398911
display id: range2398911
version: 1
direction: direction/reverse
top level: BBa_K1497016
start: 1
end: 35
orientation: inline

BBa_K1497001
type: ComponentDefinition
persistent identity: BBa K1497001
display id: BBa_K1497001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CHS - Chalcone Synthase from Gerbera
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway.
mutable notes: no
mutable provenance: Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone. We used the CHS and verified the function in our Naringenin Operon (K1497007).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497001_sequence/1

BBa_K1497015
type: ComponentDefinition
persistent identity: BBa K1497015
display id: BBa_K1497015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pelargonidin producing operon - T7-B0034-F3H-B0034-DFR-B0034-eANS
creator: Sascha Hein, Sebastian Barthel, Thomas Dohmen, Sven Jager
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1497012
component: BBa_K1497002
component: BBa_I712074
component: BBa_K1497013
sequence annotation: BBa_K1497012
sequence annotation: BBa_K1497002
sequence annotation: BBa_K1497013
sequence annotation: BBa_I712074
sequence: https://synbiohub.org/public/igem/BBa_K1497015_sequence/1

BBa_K1497012
type: Component
persistent identity: component2421896
display id: component2421896
version: 1
top level: BBa_K1497015
access: public
definition: BBa_K1497012

BBa_K1497002
type: Component
persistent identity: component2421901
display id: component2421901
version: 1
top level: BBa_K1497015
access: public
definition: BBa_K1497002

BBa_I712074
type: Component
persistent identity: component2421892
display id: component2421892
version: 1
top level: BBa_K1497015
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_K1497013
type: Component
persistent identity: component2421900
display id: component2421900
version: 1
top level: BBa_K1497015
access: public
definition: BBa_K1497013

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2421896
display id: annotation2421896
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421896/range2421896/1
component: BBa_K1497012

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type: Range
persistent identity: range2421896
display id: range2421896
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 55
end: 1179
orientation: inline

BBa_K1497002
type: SequenceAnnotation
persistent identity: annotation2421901
display id: annotation2421901
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421901/range2421901/1
component: BBa_K1497002

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type: Range
persistent identity: range2421901
display id: range2421901
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 2276
end: 3243
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2421900
display id: annotation2421900
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421900/range2421900/1
component: BBa_K1497013

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type: Range
persistent identity: range2421900
display id: range2421900
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 1188
end: 2267
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2421892
display id: annotation2421892
version: 1
top level: BBa_K1497015
location: https://synbiohub.org/public/igem/BBa_K1497015/annotation2421892/range2421892/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K1497015/annotation2421892/range2421892/1
type: Range
persistent identity: range2421892
display id: range2421892
version: 1
direction: direction/reverse
top level: BBa_K1497015
start: 1
end: 46
orientation: inline

BBa_K1497002
type: ComponentDefinition
persistent identity: BBa K1497002
display id: BBa_K1497002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-eANS (engineered anthocyanidin synthase with strong RBS)
creator: Sascha Hein, Sven Jager, Thomas Dohmen, Sven Rumpf
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: bablabla e. coli optimiert etcc...
mutable notes: Gene was E. coli optimated synthezied by Eurofins.
mutable provenance: Fragia anassana
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497002_sequence/1

BBa_K1497012
type: ComponentDefinition
persistent identity: BBa K1497012
display id: BBa_K1497012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-F3H
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_K1497009
sequence annotation: BBa_B0034
sequence annotation: BBa_K1497009
sequence: https://synbiohub.org/public/igem/BBa_K1497012_sequence/1

BBa_B0034
type: Component
persistent identity: component2398819
display id: component2398819
version: 1
top level: BBa_K1497012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497009
type: Component
persistent identity: component2398820
display id: component2398820
version: 1
top level: BBa_K1497012
access: public
definition: BBa_K1497009

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398819
display id: annotation2398819
version: 1
top level: BBa_K1497012
location: https://synbiohub.org/public/igem/BBa_K1497012/annotation2398819/range2398819/1
component: BBa_B0034

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type: Range
persistent identity: range2398819
display id: range2398819
version: 1
direction: direction/reverse
top level: BBa_K1497012
start: 1
end: 12
orientation: inline

BBa_K1497009
type: SequenceAnnotation
persistent identity: annotation2398820
display id: annotation2398820
version: 1
top level: BBa_K1497012
location: https://synbiohub.org/public/igem/BBa_K1497012/annotation2398820/range2398820/1
component: BBa_K1497009

https://synbiohub.org/public/igem/BBa_K1497012/annotation2398820/range2398820/1
type: Range
persistent identity: range2398820
display id: range2398820
version: 1
direction: direction/reverse
top level: BBa_K1497012
start: 19
end: 1125
orientation: inline

BBa_K1497017
type: ComponentDefinition
persistent identity: BBa K1497017
display id: BBa_K1497017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin producing operon under the T7 promoter BBa_I712074
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Transformed into BL21(DE3) and induced with IPTG this composite is able to produce naringenin from tyrosine. It consists of 4 genes: 4-CL (BBa_K801093) TAL (BBa_K1033000) CHI (BBa_K1497000) CHS (BBa_K1497001) For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented. For characterization the naringenin biosensor K1497020 was used. After 24 h incubation GFP fluorescence was visible to the unaided eye. We measured the relative GFP fluorescence by use of OptimaFluoroStar.
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I712074
component: BBa_K1497007
sequence annotation: BBa_K1497007
sequence annotation: BBa_I712074
sequence: https://synbiohub.org/public/igem/BBa_K1497017_sequence/1

BBa_I712074
type: Component
persistent identity: component2398886
display id: component2398886
version: 1
top level: BBa_K1497017
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_K1497007
type: Component
persistent identity: component2398904
display id: component2398904
version: 1
top level: BBa_K1497017
access: public
definition: BBa_K1497007

BBa_K1497007
type: SequenceAnnotation
persistent identity: annotation2398904
display id: annotation2398904
version: 1
top level: BBa_K1497017
location: https://synbiohub.org/public/igem/BBa_K1497017/annotation2398904/range2398904/1
component: BBa_K1497007

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type: Range
persistent identity: range2398904
display id: range2398904
version: 1
direction: direction/reverse
top level: BBa_K1497017
start: 55
end: 5357
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2398886
display id: annotation2398886
version: 1
top level: BBa_K1497017
location: https://synbiohub.org/public/igem/BBa_K1497017/annotation2398886/range2398886/1
component: BBa_I712074

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type: Range
persistent identity: range2398886
display id: range2398886
version: 1
direction: direction/reverse
top level: BBa_K1497017
start: 1
end: 46
orientation: inline

BBa_K1497009
type: ComponentDefinition
persistent identity: BBa K1497009
display id: BBa_K1497009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F3H
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497009_sequence/1

BBa_K1497014
type: ComponentDefinition
persistent identity: BBa K1497014
display id: BBa_K1497014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pelargonidin producing operon (low yield)- T7-B0034-F3H-B0034-DFR-B0034-ANS
creator: Sascha Hein, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: s
mutable notes: s
mutable provenance: s
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1497012
component: BBa_K1497013
component: BBa_K1497011
component: BBa_I712074
sequence annotation: BBa_I712074
sequence annotation: BBa_K1497013
sequence annotation: BBa_K1497012
sequence annotation: BBa_K1497011
sequence: https://synbiohub.org/public/igem/BBa_K1497014_sequence/1

BBa_K1497012
type: Component
persistent identity: component2398852
display id: component2398852
version: 1
top level: BBa_K1497014
access: public
definition: BBa_K1497012

BBa_K1497013
type: Component
persistent identity: component2398856
display id: component2398856
version: 1
top level: BBa_K1497014
access: public
definition: BBa_K1497013

BBa_K1497011
type: Component
persistent identity: component2398857
display id: component2398857
version: 1
top level: BBa_K1497014
access: public
definition: BBa_K1497011

BBa_I712074
type: Component
persistent identity: component2398848
display id: component2398848
version: 1
top level: BBa_K1497014
access: public
definition: https://synbiohub.org/public/igem/BBa_I712074/1

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2398848
display id: annotation2398848
version: 1
top level: BBa_K1497014
location: https://synbiohub.org/public/igem/BBa_K1497014/annotation2398848/range2398848/1
component: BBa_I712074

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type: Range
persistent identity: range2398848
display id: range2398848
version: 1
direction: direction/reverse
top level: BBa_K1497014
start: 1
end: 46
orientation: inline

BBa_K1497013
type: SequenceAnnotation
persistent identity: annotation2398856
display id: annotation2398856
version: 1
top level: BBa_K1497014
location: https://synbiohub.org/public/igem/BBa_K1497014/annotation2398856/range2398856/1
component: BBa_K1497013

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type: Range
persistent identity: range2398856
display id: range2398856
version: 1
direction: direction/reverse
top level: BBa_K1497014
start: 1188
end: 2267
orientation: inline

BBa_K1497012
type: SequenceAnnotation
persistent identity: annotation2398852
display id: annotation2398852
version: 1
top level: BBa_K1497014
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BBa_K1497011
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persistent identity: annotation2398857
display id: annotation2398857
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top level: BBa_K1497014
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BBa_K1497006
type: ComponentDefinition
persistent identity: BBa K1497006
display id: BBa_K1497006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-4CL-B0034-TAL
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This composite part is the fusion device of the Biobrick parts K1033001 by Uppsala 2013 and K1033000 by. It is able to produce 4-coumaroyl-CoA from tyrosine, if put under a promotor. This device is the first composite part for the assembly of our Naringenin Operon (K1497007). We used the previously announced Biobricks to assemble this part and verified the function in our Naringenin Operon (K1497007).
mutable notes: no
mutable provenance: w
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1033001
component: BBa_K1033000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1033000
sequence annotation: BBa_K1033001
sequence: https://synbiohub.org/public/igem/BBa_K1497006_sequence/1

BBa_B0034
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persistent identity: component2398655
display id: component2398655
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access: public
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BBa_B0034
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BBa_K1033001
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display id: component2398658
version: 1
top level: BBa_K1497006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033001/1

BBa_K1033000
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persistent identity: component2398663
display id: component2398663
version: 1
top level: BBa_K1497006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033000/1

BBa_B0034
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persistent identity: annotation2398655
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BBa_B0034
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BBa_K1033001
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persistent identity: annotation2398658
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