//cds/enzyme/ligase
type: Collection
persistent identity: cds enzyme ligase
display id: cds_enzyme_ligase
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K525009
member: BBa_K525010
member: BBa_K112112
member: BBa_K112114
member: SgsE|LigA
member: BBa_M1845
member: LigA
member: SbpA|LigA
member: BBa_K112113
member: BBa_K112111

LigA
type: ComponentDefinition
persistent identity: BBa K525740
display id: BBa_K525740
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LigA4 Freiburg Prefix, Suffix Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: LigA
sequence annotation: NgoMIV
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K525740_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2137503
display id: annotation2137503
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137503/range2137503/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137503/range2137503/1
type: Range
persistent identity: range2137503
display id: range2137503
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 3
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2137504
display id: annotation2137504
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137504/range2137504/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137504/range2137504/1
type: Range
persistent identity: range2137504
display id: range2137504
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 2018
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2137456
display id: annotation2137456
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137456/range2137456/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137456/range2137456/1
type: Range
persistent identity: range2137456
display id: range2137456
version: 1
direction: direction/forward
top level: LigA
start: 4
end: 9
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2137505
display id: annotation2137505
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137505/range2137505/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137505/range2137505/1
type: Range
persistent identity: range2137505
display id: range2137505
version: 1
direction: direction/forward
top level: LigA
start: 2020
end: 2037
orientation: inline

BBa_K112113
type: ComponentDefinition
persistent identity: BBa K112113
display id: BBa_K112113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ligase without start or stop codons
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv022/mv023 on pBca1256-K112109 (1489bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112113 ----------------------------------------------- mv022 Forward Biobricking of <ligase! ccataGAATTCatgAGATCTattcttaaaattctgaacg mv023 Reverse Biobricking of ligase> cgttaGGATCCtagaccagttacctcatg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112113_sequence/1

BBa_K525009
type: ComponentDefinition
persistent identity: BBa K525009
display id: BBa_K525009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and LigA with His-Tag
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and LigA with His-Tag
mutable notes: This part was assembled by Freiburg Standard Assembly (RFC 25)
mutable provenance: SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NogMIV
sequence annotation: lac operator
sequence annotation: ATG
sequence annotation: K525740
sequence annotation: K525303
sequence annotation: Freiburg scar
sequence annotation: T7 promotor
sequence annotation: B0034
sequence annotation: SgsE domain
sequence annotation: His-Tag
sequence annotation: LigA domain
sequence: https://synbiohub.org/public/igem/BBa_K525009_sequence/1

NogMIV
type: SequenceAnnotation
persistent identity: annotation2160514
display id: annotation2160514
version: 1
top level: BBa_K525009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160514/range2160514/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160514/range2160514/1
type: Range
persistent identity: range2160514
display id: range2160514
version: 1
direction: direction/forward
top level: BBa_K525009
start: 76
end: 82
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160510
display id: annotation2160510
version: 1
top level: BBa_K525009
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160510/range2160510/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160510/range2160510/1
type: Range
persistent identity: range2160510
display id: range2160510
version: 1
direction: direction/forward
top level: BBa_K525009
start: 20
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160512
display id: annotation2160512
version: 1
top level: BBa_K525009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160512/range2160512/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160512/range2160512/1
type: Range
persistent identity: range2160512
display id: range2160512
version: 1
direction: direction/forward
top level: BBa_K525009
start: 73
end: 75
orientation: inline

K525740
type: SequenceAnnotation
persistent identity: annotation2160516
display id: annotation2160516
version: 1
top level: BBa_K525009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160516/range2160516/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160516/range2160516/1
type: Range
persistent identity: range2160516
display id: range2160516
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2410
end: 4434
orientation: inline

K525303
type: SequenceAnnotation
persistent identity: annotation2160509
display id: annotation2160509
version: 1
top level: BBa_K525009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160509/range2160509/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160509/range2160509/1
type: Range
persistent identity: range2160509
display id: range2160509
version: 1
direction: direction/forward
top level: BBa_K525009
start: 1
end: 2409
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160515
display id: annotation2160515
version: 1
top level: BBa_K525009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160515/range2160515/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160515/range2160515/1
type: Range
persistent identity: range2160515
display id: range2160515
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2401
end: 2407
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2160508
display id: annotation2160508
version: 1
top level: BBa_K525009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160508/range2160508/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160508/range2160508/1
type: Range
persistent identity: range2160508
display id: range2160508
version: 1
direction: direction/forward
top level: BBa_K525009
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160511
display id: annotation2160511
version: 1
top level: BBa_K525009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160511/range2160511/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160511/range2160511/1
type: Range
persistent identity: range2160511
display id: range2160511
version: 1
direction: direction/forward
top level: BBa_K525009
start: 53
end: 64
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2160513
display id: annotation2160513
version: 1
top level: BBa_K525009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160513/range2160513/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160513/range2160513/1
type: Range
persistent identity: range2160513
display id: range2160513
version: 1
direction: direction/forward
top level: BBa_K525009
start: 73
end: 2409
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2160518
display id: annotation2160518
version: 1
top level: BBa_K525009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160518/range2160518/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160518/range2160518/1
type: Range
persistent identity: range2160518
display id: range2160518
version: 1
direction: direction/forward
top level: BBa_K525009
start: 4417
end: 4434
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160517
display id: annotation2160517
version: 1
top level: BBa_K525009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160517/range2160517/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160517/range2160517/1
type: Range
persistent identity: range2160517
display id: range2160517
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2407
end: 4416
orientation: inline

SbpA|LigA
type: ComponentDefinition
persistent identity: BBa K525006
display id: BBa_K525006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and LigA
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion Protein of S-Layer SbpA and LigA
mutable notes: Assembled via Freiburg Standard Assembly (RFC 25)
mutable provenance: Assembled fusion protein. SbpA was synthesized, LigA is the natural ligase A of E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: ATG
sequence annotation: T7 Promotor
sequence annotation: K525401
sequence annotation: SbpA domain
sequence annotation: lac operator
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: LigA domain
sequence annotation: Freiburg Scar
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: K525720
sequence: https://synbiohub.org/public/igem/BBa_K525006_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2160490
display id: annotation2160490
version: 1
top level: SbpA|LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160490/range2160490/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160490/range2160490/1
type: Range
persistent identity: range2160490
display id: range2160490
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 73
end: 75
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2160486
display id: annotation2160486
version: 1
top level: SbpA|LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160486/range2160486/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160486/range2160486/1
type: Range
persistent identity: range2160486
display id: range2160486
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 1
end: 20
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2160485
display id: annotation2160485
version: 1
top level: SbpA|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160485/range2160485/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160485/range2160485/1
type: Range
persistent identity: range2160485
display id: range2160485
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 73
end: 3193
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160489
display id: annotation2160489
version: 1
top level: SbpA|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160489/range2160489/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160489/range2160489/1
type: Range
persistent identity: range2160489
display id: range2160489
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 73
end: 3192
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160487
display id: annotation2160487
version: 1
top level: SbpA|LigA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160487/range2160487/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160487/range2160487/1
type: Range
persistent identity: range2160487
display id: range2160487
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 20
end: 44
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160495
display id: annotation2160495
version: 1
top level: SbpA|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160495/range2160495/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160495/range2160495/1
type: Range
persistent identity: range2160495
display id: range2160495
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 5209
end: 5215
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161006
display id: annotation2161006
version: 1
top level: SbpA|LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2161006/range2161006/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2161006/range2161006/1
type: Range
persistent identity: range2161006
display id: range2161006
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160488
display id: annotation2160488
version: 1
top level: SbpA|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160488/range2160488/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160488/range2160488/1
type: Range
persistent identity: range2160488
display id: range2160488
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 53
end: 64
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160493
display id: annotation2160493
version: 1
top level: SbpA|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160493/range2160493/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160493/range2160493/1
type: Range
persistent identity: range2160493
display id: range2160493
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 3199
end: 5208
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160491
display id: annotation2160491
version: 1
top level: SbpA|LigA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160491/range2160491/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160491/range2160491/1
type: Range
persistent identity: range2160491
display id: range2160491
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 3193
end: 3198
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160484
display id: annotation2160484
version: 1
top level: SbpA|LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160484/range2160484/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160484/range2160484/1
type: Range
persistent identity: range2160484
display id: range2160484
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 5214
end: 5217
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160494
display id: annotation2160494
version: 1
top level: SbpA|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160494/range2160494/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160494/range2160494/1
type: Range
persistent identity: range2160494
display id: range2160494
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 76
end: 82
orientation: inline

K525720
type: SequenceAnnotation
persistent identity: annotation2160492
display id: annotation2160492
version: 1
top level: SbpA|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160492/range2160492/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160492/range2160492/1
type: Range
persistent identity: range2160492
display id: range2160492
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 3194
end: 5217
orientation: inline

BBa_K112112
type: ComponentDefinition
persistent identity: BBa K112112
display id: BBa_K112112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ligase with a start codon without a stop codon
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv021/mv023 on pBca1256-K112109 (1492bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112112 ----------------------------------------------- mv021 Forward Biobricking of ligase! ccataGAATTCatgAGATCTatgattcttaaaattctgaacg mv023 Reverse Biobricking of ligase> cgttaGGATCCtagaccagttacctcatg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112112_sequence/1

BBa_M1845
type: ComponentDefinition
persistent identity: BBa M1845
display id: BBa_M1845
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His6
creator: sarah guzman
created: 2012-03-26T11:00:00Z
modified: 2015-05-08T01:13:58Z
discontinued: true
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 12257
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: His6
mutable notes: catcatcaccatcaccac
mutable provenance: Hus6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: stop
sequence annotation: BBa_M1872
sequence annotation: operator
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_M1845_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2171245
display id: annotation2171245
version: 1
top level: BBa_M1845
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171245/range2171245/1

https://synbiohub.org/public/igem/BBa_M1845/annotation2171245/range2171245/1
type: Range
persistent identity: range2171245
display id: range2171245
version: 1
direction: direction/forward
top level: BBa_M1845
start: 1
end: 200
orientation: inline

BBa_M1872
type: SequenceAnnotation
persistent identity: annotation2171247
display id: annotation2171247
version: 1
top level: BBa_M1845
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171247/range2171247/1

https://synbiohub.org/public/igem/BBa_M1845/annotation2171247/range2171247/1
type: Range
persistent identity: range2171247
display id: range2171247
version: 1
direction: direction/reverse
top level: BBa_M1845
start: 1
end: 717
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation2171246
display id: annotation2171246
version: 1
top level: BBa_M1845
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171246/range2171246/1

https://synbiohub.org/public/igem/BBa_M1845/annotation2171246/range2171246/1
type: Range
persistent identity: range2171246
display id: range2171246
version: 1
direction: direction/forward
top level: BBa_M1845
start: 300
end: 500
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2171244
display id: annotation2171244
version: 1
top level: BBa_M1845
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M1845/annotation2171244/range2171244/1

https://synbiohub.org/public/igem/BBa_M1845/annotation2171244/range2171244/1
type: Range
persistent identity: range2171244
display id: range2171244
version: 1
direction: direction/forward
top level: BBa_M1845
start: 1
end: 299
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525710
display id: BBa_K525710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: LigA1 mit Promotor, RBS, Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: LigA
sequence annotation: RBS
sequence annotation: His-tag
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K525710_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2161302
display id: annotation2161302
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1
type: Range
persistent identity: range2161302
display id: range2161302
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161299
display id: annotation2161299
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1
type: Range
persistent identity: range2161299
display id: range2161299
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2161303
display id: annotation2161303
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1
type: Range
persistent identity: range2161303
display id: range2161303
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2045
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161301
display id: annotation2161301
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1
type: Range
persistent identity: range2161301
display id: range2161301
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2161304
display id: annotation2161304
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1
type: Range
persistent identity: range2161304
display id: range2161304
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2064
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161300
display id: annotation2161300
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1
type: Range
persistent identity: range2161300
display id: range2161300
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline

BBa_K112111
type: ComponentDefinition
persistent identity: BBa K112111
display id: BBa_K112111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ligase without a start with a stop codon.
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv022/mv014 on pBca1256-K112109 (1492bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112111 ----------------------------------------------- mv022 Forward Biobricking of <ligase! ccataGAATTCatgAGATCTattcttaaaattctgaacg mv014 Reverse Biobricking of ligase! cgttaGGATCCtcatagaccagttacctcatg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112111_sequence/1

SgsE|LigA
type: ComponentDefinition
persistent identity: BBa K525005
display id: BBa_K525005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and LigA
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || LigA4 ; RCU 25
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K525740
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: SgsE domain
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: lac operator
sequence annotation: K525301
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: Freiburg scar
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: LigA domain
sequence annotation: K525720
sequence: https://synbiohub.org/public/igem/BBa_K525005_sequence/1

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2160916
display id: annotation2160916
version: 1
top level: SgsE|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160916/range2160916/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160916/range2160916/1
type: Range
persistent identity: range2160916
display id: range2160916
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 73
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160914
display id: annotation2160914
version: 1
top level: SgsE|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160914/range2160914/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160914/range2160914/1
type: Range
persistent identity: range2160914
display id: range2160914
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 76
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160918
display id: annotation2160918
version: 1
top level: SgsE|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160918/range2160918/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160918/range2160918/1
type: Range
persistent identity: range2160918
display id: range2160918
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 4417
end: 4422
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160910
display id: annotation2160910
version: 1
top level: SgsE|LigA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160910/range2160910/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160910/range2160910/1
type: Range
persistent identity: range2160910
display id: range2160910
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 20
end: 44
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2160915
display id: annotation2160915
version: 1
top level: SgsE|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160915/range2160915/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160915/range2160915/1
type: Range
persistent identity: range2160915
display id: range2160915
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 73
end: 2400
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160919
display id: annotation2160919
version: 1
top level: SgsE|LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160919/range2160919/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160919/range2160919/1
type: Range
persistent identity: range2160919
display id: range2160919
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 4423
end: 4425
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160911
display id: annotation2160911
version: 1
top level: SgsE|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160911/range2160911/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160911/range2160911/1
type: Range
persistent identity: range2160911
display id: range2160911
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 53
end: 64
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160917
display id: annotation2160917
version: 1
top level: SgsE|LigA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160917/range2160917/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160917/range2160917/1
type: Range
persistent identity: range2160917
display id: range2160917
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 2401
end: 2406
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160912
display id: annotation2160912
version: 1
top level: SgsE|LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160912/range2160912/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160912/range2160912/1
type: Range
persistent identity: range2160912
display id: range2160912
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 56
end: 59
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160913
display id: annotation2160913
version: 1
top level: SgsE|LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160913/range2160913/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160913/range2160913/1
type: Range
persistent identity: range2160913
display id: range2160913
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 73
end: 75
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160909
display id: annotation2160909
version: 1
top level: SgsE|LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160909/range2160909/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160909/range2160909/1
type: Range
persistent identity: range2160909
display id: range2160909
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 1
end: 20
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2161007
display id: annotation2161007
version: 1
top level: SgsE|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2161007/range2161007/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2161007/range2161007/1
type: Range
persistent identity: range2161007
display id: range2161007
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 2407
end: 4425
orientation: inline

K525720
type: SequenceAnnotation
persistent identity: annotation2160943
display id: annotation2160943
version: 1
top level: SgsE|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160943/range2160943/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160943/range2160943/1
type: Range
persistent identity: range2160943
display id: range2160943
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 2407
end: 4425
orientation: inline

BBa_K525010
type: ComponentDefinition
persistent identity: BBa K525010
display id: BBa_K525010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and LigA with His-Tag
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of S-Layer SbpA and LigA with His-Tag
mutable notes: Assembled with Freiburg Standard Assembly (RFC 25)
mutable provenance: SbpA was synthesized, LigA is the native ligase of E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: Freiburg scar
sequence annotation: B0034
sequence annotation: T7 Promotor
sequence annotation: ATG
sequence annotation: K525403
sequence annotation: lac operator
sequence annotation: K525740
sequence annotation: SbpA domain
sequence annotation: His-Tag
sequence annotation: LigA domain
sequence: https://synbiohub.org/public/igem/BBa_K525010_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160529
display id: annotation2160529
version: 1
top level: BBa_K525010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160529/range2160529/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160529/range2160529/1
type: Range
persistent identity: range2160529
display id: range2160529
version: 1
direction: direction/forward
top level: BBa_K525010
start: 76
end: 81
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160523
display id: annotation2160523
version: 1
top level: BBa_K525010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160523/range2160523/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160523/range2160523/1
type: Range
persistent identity: range2160523
display id: range2160523
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3193
end: 3199
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160521
display id: annotation2160521
version: 1
top level: BBa_K525010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160521/range2160521/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160521/range2160521/1
type: Range
persistent identity: range2160521
display id: range2160521
version: 1
direction: direction/forward
top level: BBa_K525010
start: 53
end: 64
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2160519
display id: annotation2160519
version: 1
top level: BBa_K525010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160519/range2160519/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160519/range2160519/1
type: Range
persistent identity: range2160519
display id: range2160519
version: 1
direction: direction/forward
top level: BBa_K525010
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160522
display id: annotation2160522
version: 1
top level: BBa_K525010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160522/range2160522/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160522/range2160522/1
type: Range
persistent identity: range2160522
display id: range2160522
version: 1
direction: direction/forward
top level: BBa_K525010
start: 73
end: 75
orientation: inline

K525403
type: SequenceAnnotation
persistent identity: annotation2160527
display id: annotation2160527
version: 1
top level: BBa_K525010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160527/range2160527/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160527/range2160527/1
type: Range
persistent identity: range2160527
display id: range2160527
version: 1
direction: direction/forward
top level: BBa_K525010
start: 1
end: 3193
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160520
display id: annotation2160520
version: 1
top level: BBa_K525010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160520/range2160520/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160520/range2160520/1
type: Range
persistent identity: range2160520
display id: range2160520
version: 1
direction: direction/forward
top level: BBa_K525010
start: 20
end: 44
orientation: inline

K525740
type: SequenceAnnotation
persistent identity: annotation2160528
display id: annotation2160528
version: 1
top level: BBa_K525010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160528/range2160528/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160528/range2160528/1
type: Range
persistent identity: range2160528
display id: range2160528
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3189
end: 5226
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160524
display id: annotation2160524
version: 1
top level: BBa_K525010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160524/range2160524/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160524/range2160524/1
type: Range
persistent identity: range2160524
display id: range2160524
version: 1
direction: direction/forward
top level: BBa_K525010
start: 73
end: 3202
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2160526
display id: annotation2160526
version: 1
top level: BBa_K525010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160526/range2160526/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160526/range2160526/1
type: Range
persistent identity: range2160526
display id: range2160526
version: 1
direction: direction/forward
top level: BBa_K525010
start: 5209
end: 5226
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160525
display id: annotation2160525
version: 1
top level: BBa_K525010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160525/range2160525/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160525/range2160525/1
type: Range
persistent identity: range2160525
display id: range2160525
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3199
end: 5208
orientation: inline

BBa_K112114
type: ComponentDefinition
persistent identity: BBa K112114
display id: BBa_K112114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~Ligase!
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv024/mv014 on pBca1256-K112109 (1499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112114 ----------------------------------------------- mv024 Forward Biobricking of a~ligase! ccataGAATTCatgAGATCTaacaatgattcttaaaattctgaacg mv014 Reverse Biobricking of ligase! cgttaGGATCCtcatagaccagttacctcatg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112114_sequence/1

LigA
type: ComponentDefinition
persistent identity: BBa K525720
display id: BBa_K525720
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA)
creator: Anna Drong
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ligase A im Freiburg Standard
mutable notes: PCR from Escherichia coli
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: NgoMIV
sequence annotation: Lig A
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525720_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129482
display id: annotation2129482
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129482/range2129482/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129482/range2129482/1
type: Range
persistent identity: range2129482
display id: range2129482
version: 1
direction: direction/forward
top level: LigA
start: 4
end: 9
orientation: inline

Lig A
type: SequenceAnnotation
persistent identity: annotation2129504
display id: annotation2129504
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129504/range2129504/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129504/range2129504/1
type: Range
persistent identity: range2129504
display id: range2129504
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 2028
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129494
display id: annotation2129494
version: 1
top level: LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129494/range2129494/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129494/range2129494/1
type: Range
persistent identity: range2129494
display id: range2129494
version: 1
direction: direction/forward
top level: LigA
start: 2026
end: 2028
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129483
display id: annotation2129483
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129483/range2129483/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129483/range2129483/1
type: Range
persistent identity: range2129483
display id: range2129483
version: 1
direction: direction/forward
top level: LigA
start: 2020
end: 2025
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2126908
display id: annotation2126908
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2126908/range2126908/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2126908/range2126908/1
type: Range
persistent identity: range2126908
display id: range2126908
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 3
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525730
display id: BBa_K525730
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7 and RBS
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LigA3 Promotor, RBS, Freiburg Suffix
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: LigA
sequence annotation: T7 promoter
sequence annotation: TAA
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K525730_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2161298
display id: annotation2161298
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2161298/range2161298/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2161298/range2161298/1
type: Range
persistent identity: range2161298
display id: range2161298
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2137444
display id: annotation2137444
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137444/range2137444/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137444/range2137444/1
type: Range
persistent identity: range2137444
display id: range2137444
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2052
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161297
display id: annotation2161297
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2161297/range2161297/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2161297/range2161297/1
type: Range
persistent identity: range2161297
display id: range2161297
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2137451
display id: annotation2137451
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137451/range2137451/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137451/range2137451/1
type: Range
persistent identity: range2137451
display id: range2137451
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2055
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2137443
display id: annotation2137443
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137443/range2137443/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137443/range2137443/1
type: Range
persistent identity: range2137443
display id: range2137443
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2137445
display id: annotation2137445
version: 1
top level: LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137445/range2137445/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137445/range2137445/1
type: Range
persistent identity: range2137445
display id: range2137445
version: 1
direction: direction/forward
top level: LigA
start: 2053
end: 2055
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2137450
display id: annotation2137450
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137450/range2137450/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137450/range2137450/1
type: Range
persistent identity: range2137450
display id: range2137450
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline