DNA ligase from Escherichia coli (LigA) with His-tag
---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
PCR mv022/mv023 on pBca1256-K112109 (1489bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112113 ----------------------------------------------- mv022 Forward Biobricking of <ligase! ccataGAATTCatgAGATCTattcttaaaattctgaacg mv023 Reverse Biobricking of ligase> cgttaGGATCCtagaccagttacctcatg
Fusion Protein of S-Layer SgsE and LigA with His-Tag
This part was assembled by Freiburg Standard Assembly (RFC 25)
SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.
Assembled via Freiburg Standard Assembly (RFC 25)
Assembled fusion protein. SbpA was synthesized, LigA is the natural ligase A of E. coli
Ligase with a start codon without a stop codon
PCR mv021/mv023 on pBca1256-K112109 (1492bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112112 ----------------------------------------------- mv021 Forward Biobricking of ligase! ccataGAATTCatgAGATCTatgattcttaaaattctgaacg mv023 Reverse Biobricking of ligase> cgttaGGATCCtagaccagttacctcatg
DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
Ligase without a start with a stop codon.
PCR mv022/mv014 on pBca1256-K112109 (1492bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112111 ----------------------------------------------- mv022 Forward Biobricking of <ligase! ccataGAATTCatgAGATCTattcttaaaattctgaacg mv014 Reverse Biobricking of ligase! cgttaGGATCCtcatagaccagttacctcatg
Fusion Protein of S-Layer SbpA and LigA with His-Tag
Assembled with Freiburg Standard Assembly (RFC 25)
SbpA was synthesized, LigA is the native ligase of E. coli.
PCR mv024/mv014 on pBca1256-K112109 (1499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112114 ----------------------------------------------- mv024 Forward Biobricking of a~ligase! ccataGAATTCatgAGATCTaacaatgattcttaaaattctgaacg mv014 Reverse Biobricking of ligase! cgttaGGATCCtcatagaccagttacctcatg
DNA ligase from Escherichia coli (LigA) with PT7 and RBS